##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939356_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2039068 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.906353785160672 33.0 31.0 34.0 30.0 34.0 2 31.9573898467339 33.0 31.0 34.0 30.0 34.0 3 31.99441950930523 34.0 31.0 34.0 30.0 34.0 4 35.52468039319925 37.0 35.0 37.0 33.0 37.0 5 35.57451394460607 37.0 35.0 37.0 33.0 37.0 6 35.687763723426585 37.0 35.0 37.0 33.0 37.0 7 35.61004292157005 37.0 35.0 37.0 33.0 37.0 8 35.595618684614735 37.0 35.0 37.0 33.0 37.0 9 37.21055894163412 39.0 37.0 39.0 34.0 39.0 10-11 37.119169885457474 39.0 37.0 39.0 33.0 39.0 12-13 37.007652270547126 39.0 37.0 39.0 33.0 39.0 14-15 38.189488285824694 40.0 38.0 41.0 33.0 41.0 16-17 38.14636073931816 40.0 38.0 41.0 33.0 41.0 18-19 38.14009022749609 40.0 38.0 41.0 33.0 41.0 20-21 38.06848496469956 40.0 38.0 41.0 33.0 41.0 22-23 37.97822362961902 40.0 38.0 41.0 33.0 41.0 24-25 37.98133093158247 40.0 38.0 41.0 33.0 41.0 26-27 37.88604818475892 40.0 38.0 41.0 33.0 41.0 28-29 37.729245174756315 40.0 37.5 41.0 32.5 41.0 30-31 37.485896497811744 40.0 37.0 41.0 32.0 41.0 32-33 37.22267526144297 40.0 36.0 41.0 31.5 41.0 34-35 36.88991980649983 40.0 35.5 41.0 31.0 41.0 36-37 36.60858367646395 39.0 35.0 41.0 30.0 41.0 38-39 36.255496874062075 39.0 35.0 41.0 30.0 41.0 40-41 36.06514986258428 39.0 35.0 41.0 30.0 41.0 42-43 35.830858509868236 39.0 35.0 41.0 28.5 41.0 44-45 35.634538671589176 39.0 35.0 41.0 27.5 41.0 46-47 35.28880155051229 39.0 35.0 41.0 25.0 41.0 48-49 34.98419743726055 39.0 35.0 41.0 24.0 41.0 50-51 33.79918325431031 38.0 33.5 40.0 22.0 40.5 52-53 33.8905316056159 38.0 33.5 39.5 22.0 40.5 54-55 34.351894100638134 38.0 34.0 40.0 22.0 41.0 56-57 34.2353057867614 38.0 34.0 40.0 21.5 41.0 58-59 34.02321575347169 38.0 34.0 40.0 20.5 41.0 60-61 33.72382897480614 38.0 34.0 40.0 19.5 41.0 62-63 33.35836592992485 37.0 33.5 40.0 17.5 41.0 64-65 32.982194316226824 36.5 33.0 40.0 13.5 41.0 66-67 32.627048239685976 36.0 33.0 39.0 10.5 41.0 68-69 32.21644471886175 35.5 32.0 39.0 7.5 41.0 70-71 31.806776919651526 35.0 32.0 38.5 6.0 40.0 72-73 31.371978766769914 35.0 31.5 37.5 2.0 40.0 74-75 30.9321371332393 35.0 31.0 37.0 2.0 39.0 76-77 30.491846029656685 35.0 31.0 36.5 2.0 39.0 78-79 30.06120222572273 35.0 30.0 36.0 2.0 38.5 80-81 29.602152061628153 34.5 30.0 35.5 2.0 37.0 82-83 29.157511421884898 34.0 29.0 35.0 2.0 37.0 84-85 28.740575105881707 34.0 29.0 35.0 2.0 36.5 86-87 28.40027797013145 34.0 29.0 35.0 2.0 36.0 88-89 27.984254325995995 34.0 28.0 35.0 2.0 36.0 90-91 27.582428589924415 34.0 27.0 35.0 2.0 35.0 92-93 27.163735098584255 34.0 26.0 35.0 2.0 35.0 94-95 26.67537301355325 33.5 25.0 35.0 2.0 35.0 96-97 26.098226738882666 33.0 24.5 35.0 2.0 35.0 98-99 25.364826724758565 33.0 19.0 35.0 2.0 35.0 100-101 22.959311803235593 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38363092818208955 1101 2 0.19522169105461984 1101 3 0.3900856369245034 1101 4 0.47989718433045425 1101 5 0.46627792039137717 1101 6 0.42350855832390266 1101 7 0.4003295546469161 1101 8 0.37509466651049195 1101 9 0.5524368308768643 1101 10-11 0.6161707407951909 1101 12-13 0.5528948804991742 1101 14-15 0.5701953925106906 1101 16-17 0.729585787662927 1101 18-19 0.7516474510908182 1101 20-21 0.7376916121170467 1101 22-23 0.7756045245369592 1101 24-25 0.7884665271576807 1101 26-27 0.7215733006426674 1101 28-29 0.6695545152677127 1101 30-31 0.7382422046668182 1101 32-33 0.7971270539278947 1101 34-35 0.7909519425635239 1101 36-37 0.8511359756320189 1101 38-39 0.9147767388819901 1101 40-41 0.8129478457311379 1101 42-43 0.6861824169792712 1101 44-45 0.9208211404149864 1101 46-47 0.749713841083846 1101 48-49 0.740768876066312 1101 50-51 0.7869917296257327 1101 52-53 0.9161362837279725 1101 54-55 0.8467616669155404 1101 56-57 0.8451324063274654 1101 58-59 0.8306378827114642 1101 60-61 0.9124144917848014 1101 62-63 1.0030899988266668 1101 64-65 0.95331075277889 1101 66-67 0.9405666601692744 1101 68-69 0.8250379299379205 1101 70-71 0.8423605945068395 1101 72-73 0.9036579851344761 1101 74-75 0.8694719411660152 1101 76-77 0.8117077731980054 1101 78-79 0.8103807514972807 1101 80-81 0.7847118881744883 1101 82-83 0.6784292277808639 1101 84-85 0.5760993684128906 1101 86-87 0.6212659179562614 1101 88-89 0.37761781753728485 1101 90-91 0.5134513891852954 1101 92-93 0.5050046520716087 1101 94-95 0.6269058052821457 1101 96-97 0.5611551361913456 1101 98-99 0.6535408101878133 1101 100-101 0.45084947866118696 1102 1 0.21423204830490405 1102 2 0.30257566948361614 1102 3 0.262336517032125 1102 4 0.18094646257213753 1102 5 0.14620959819335866 1102 6 0.14389918441867877 1102 7 0.1399740238653422 1102 8 0.25378290522090197 1102 9 0.21640745918261928 1102 10-11 0.30658227771767343 1102 12-13 0.3123088790760491 1102 14-15 0.41161611431572 1102 16-17 0.4081679930623707 1102 18-19 0.3400389055221851 1102 20-21 0.3514883987316182 1102 22-23 0.3252570747081549 1102 24-25 0.39518181448304546 1102 26-27 0.357353548551977 1102 28-29 0.3859597836129538 1102 30-31 0.43030730071183143 1102 32-33 0.322188128180791 1102 34-35 0.2953046021934824 1102 36-37 0.3318850908533264 1102 38-39 0.34032936550304527 1102 40-41 0.47366164765592345 1102 42-43 0.2537290615154433 1102 44-45 0.447442196668689 1102 46-47 0.29999029063395 1102 48-49 0.34085980624782053 1102 50-51 0.35392132568708234 1102 52-53 0.3591710162546562 1102 54-55 0.2776805755665208 1102 56-57 0.2700524142371492 1102 58-59 0.26234729227252274 1102 60-61 0.2891095845133478 1102 62-63 0.30540031052723293 1102 64-65 0.4151773750184802 1102 66-67 0.4816780013738722 1102 68-69 0.2645959843434369 1102 70-71 0.2322830702990153 1102 72-73 0.15344288231641912 1102 74-75 0.19900836851295267 1102 76-77 0.08393280671293724 1102 78-79 0.1401161664674433 1102 80-81 0.12877114226673214 1102 82-83 -0.05484390063777411 1102 84-85 0.0557486021236997 1102 86-87 -0.21569673371730502 1102 88-89 -0.20807282366062907 1102 90-91 -0.2578855180363355 1102 92-93 -0.1540165094368966 1102 94-95 -0.16024345567657505 1102 96-97 -0.3754663715992237 1102 98-99 -0.35562803501445117 1102 100-101 -0.5508515696828375 1103 1 0.2738389580242817 1103 2 0.3298992234659508 1103 3 0.3498709023408395 1103 4 0.40605999983425534 1103 5 0.3622364850890847 1103 6 0.2767451198886661 1103 7 0.2918876756126423 1103 8 0.2608425194694206 1103 9 0.2309712962956354 1103 10-11 0.3015896042805579 1103 12-13 0.25160711299522376 1103 14-15 0.46329758496115403 1103 16-17 0.333163681520773 1103 18-19 0.39088283627821596 1103 20-21 0.43520064827998084 1103 22-23 0.43531116162149175 1103 24-25 0.4036059857802883 1103 26-27 0.3493031970848932 1103 28-29 0.4173282363382782 1103 30-31 0.33981522844427303 1103 32-33 0.2909244061645282 1103 34-35 0.1494978974710861 1103 36-37 0.22513437868450836 1103 38-39 0.458417291596497 1103 40-41 0.31221291463759115 1103 42-43 0.3202769416435771 1103 44-45 0.4112818171062571 1103 46-47 0.34164757995245054 1103 48-49 0.2344260382540142 1103 50-51 0.20408485848476943 1103 52-53 0.1718855995395714 1103 54-55 0.3411398935723753 1103 56-57 0.17568469416811183 1103 58-59 0.09715607998933962 1103 60-61 0.19113912805257627 1103 62-63 0.05898041897015105 1103 64-65 0.20069224647338046 1103 66-67 0.03300919748725306 1103 68-69 0.024418960395728107 1103 70-71 0.029509281291797862 1103 72-73 0.21040935503506475 1103 74-75 0.06820078313525713 1103 76-77 0.2873323808182491 1103 78-79 0.22001881736222728 1103 80-81 -0.031136596858701182 1103 82-83 -0.056504608611252394 1103 84-85 -0.02264714969295767 1103 86-87 -0.02258309126580471 1103 88-89 -0.14294943406728322 1103 90-91 -0.12094713455253725 1103 92-93 -0.10799386069482608 1103 94-95 -0.13176205401580532 1103 96-97 -0.3227959984337687 1103 98-99 -0.7023307147751581 1103 100-101 -0.6659516891828723 1104 1 0.25700823696901054 1104 2 0.30745073844467186 1104 3 0.29766157321756026 1104 4 0.44874205743979445 1104 5 0.3945537741170426 1104 6 0.4006525129088061 1104 7 0.35648899137398615 1104 8 0.3659792868970797 1104 9 0.3771163151482284 1104 10-11 0.4123729431972123 1104 12-13 0.44152976628094365 1104 14-15 0.4023815532479347 1104 16-17 0.40957786361283155 1104 18-19 0.323515107356414 1104 20-21 0.4763927332793969 1104 22-23 0.38753581515905466 1104 24-25 0.5490581391574807 1104 26-27 0.34989300884406305 1104 28-29 0.42077563558322595 1104 30-31 0.4469456546530637 1104 32-33 0.542991123199819 1104 34-35 0.4953054822246017 1104 36-37 0.5615207154842423 1104 38-39 0.4740654192856866 1104 40-41 0.4784112363172355 1104 42-43 0.4504762540644336 1104 44-45 0.39183287829224867 1104 46-47 0.4370197850287525 1104 48-49 0.33645782987998984 1104 50-51 0.23309730095881775 1104 52-53 0.24547129865361228 1104 54-55 0.37277916636358555 1104 56-57 0.27382865813079604 1104 58-59 0.2735059351795641 1104 60-61 0.20307197623158402 1104 62-63 0.17829286358249163 1104 64-65 0.4078319980040419 1104 66-67 0.3193429085852202 1104 68-69 0.29550844309577684 1104 70-71 0.26424337300544565 1104 72-73 0.06320707039060025 1104 74-75 0.10210753466829559 1104 76-77 0.13404784802281355 1104 78-79 0.15985207823127467 1104 80-81 0.24982822351303113 1104 82-83 0.15642456408420102 1104 84-85 0.21269486985101693 1104 86-87 0.280950195975489 1104 88-89 0.07890502201104255 1104 90-91 0.08943955150129668 1104 92-93 0.14266990027282134 1104 94-95 -0.14780725034649933 1104 96-97 -0.18989026377659712 1104 98-99 -0.3482286422966183 1104 100-101 -0.5196961919947221 1105 1 0.2743600639678867 1105 2 0.2710211691740909 1105 3 0.3014789522747776 1105 4 0.26965417820466797 1105 5 0.21926611203718238 1105 6 0.14986792795796333 1105 7 0.18070649445454734 1105 8 0.2151679293791915 1105 9 0.3647940695476848 1105 10-11 0.3560229822691454 1105 12-13 0.37999891318568046 1105 14-15 0.36633412232745144 1105 16-17 0.4081239644859167 1105 18-19 0.3985452264943419 1105 20-21 0.4459377941412228 1105 22-23 0.5040619830550881 1105 24-25 0.3320118512196615 1105 26-27 0.4375182478235473 1105 28-29 0.4074497047502703 1105 30-31 0.5261541248525603 1105 32-33 0.4993198033694668 1105 34-35 0.5762038277028481 1105 36-37 0.5805707126811299 1105 38-39 0.9057930850725242 1105 40-41 0.7219529265815225 1105 42-43 0.6351534787331801 1105 44-45 0.7465691055798942 1105 46-47 0.6505927900434543 1105 48-49 0.7366456382936732 1105 50-51 0.5698407042097386 1105 52-53 0.5985053512994583 1105 54-55 0.8629511171798612 1105 56-57 0.717257880730962 1105 58-59 0.7373995817290506 1105 60-61 0.7601072691108826 1105 62-63 0.7749275290226549 1105 64-65 0.6723767099071907 1105 66-67 0.7145863730469202 1105 68-69 0.6675876303996304 1105 70-71 0.780510853832741 1105 72-73 0.8712454764389115 1105 74-75 0.8657672644941918 1105 76-77 0.7505828829015186 1105 78-79 0.7718440716319499 1105 80-81 0.8580551816574378 1105 82-83 0.750174885869864 1105 84-85 0.7552769809708977 1105 86-87 0.6599994037248926 1105 88-89 0.8018764565973093 1105 90-91 0.6889376802819207 1105 92-93 0.8949635884685243 1105 94-95 0.9447520354437877 1105 96-97 0.7046338309616793 1105 98-99 0.6271108487271349 1105 100-101 0.45945038094507495 1106 1 0.2252106603506583 1106 2 0.32214539302444223 1106 3 0.26413586743516504 1106 4 0.3490401180454512 1106 5 0.34982912454940873 1106 6 0.3295274952663334 1106 7 0.32359730339742754 1106 8 0.2265320437354177 1106 9 0.4217553917604775 1106 10-11 0.4720431316910876 1106 12-13 0.410275355661895 1106 14-15 0.4710427365774308 1106 16-17 0.49784858977159274 1106 18-19 0.5406667615260901 1106 20-21 0.48324690473870646 1106 22-23 0.5130902334463272 1106 24-25 0.40888019186375146 1106 26-27 0.39192238815119396 1106 28-29 0.4281526911400775 1106 30-31 0.3771786909259873 1106 32-33 0.36654469020288616 1106 34-35 0.226927856551832 1106 36-37 0.36899962508474715 1106 38-39 0.5781279675593964 1106 40-41 0.6053223193485664 1106 42-43 0.4581705963584781 1106 44-45 0.4653122635450231 1106 46-47 0.36753315608086723 1106 48-49 0.4411727330214106 1106 50-51 0.4643040352170047 1106 52-53 0.4627779843463671 1106 54-55 0.4665318405184351 1106 56-57 0.45359187665189893 1106 58-59 0.4108858135326017 1106 60-61 0.5577321460216567 1106 62-63 0.40952376942223623 1106 64-65 0.3782576512201956 1106 66-67 0.30211885080458245 1106 68-69 0.39679431566764123 1106 70-71 0.37568214333319006 1106 72-73 0.16265117958169029 1106 74-75 0.3696880538182974 1106 76-77 0.47353965580150614 1106 78-79 0.563189645758893 1106 80-81 0.5539886805837462 1106 82-83 0.5143448412072189 1106 84-85 0.5144562279938398 1106 86-87 0.5400959444999138 1106 88-89 0.3275741958541687 1106 90-91 0.53375628479262 1106 92-93 0.7011176179754237 1106 94-95 0.6481779333075082 1106 96-97 0.5376498916105561 1106 98-99 0.7333418325888275 1106 100-101 0.6155854819456472 1107 1 0.027268430838955027 1107 2 0.050803628833591574 1107 3 0.007723318570306503 1107 4 0.03571208739676024 1107 5 -0.03224625989522423 1107 6 -0.06573420660769358 1107 7 0.027160275860460104 1107 8 0.021619764115399676 1107 9 -0.014845669598571476 1107 10-11 0.006205112264360935 1107 12-13 0.08681775864126706 1107 14-15 0.10525820644050299 1107 16-17 0.214001584555227 1107 18-19 0.15354123793736818 1107 20-21 0.11928106183793119 1107 22-23 0.20982497178555803 1107 24-25 0.018721810128006666 1107 26-27 0.0549534787366639 1107 28-29 0.1705704173160214 1107 30-31 0.3410636576095101 1107 32-33 0.21405730977020454 1107 34-35 0.26572429101023687 1107 36-37 0.28312619276797335 1107 38-39 0.5085862387197793 1107 40-41 0.4956078730269482 1107 42-43 0.5834241695046956 1107 44-45 0.5588617429364859 1107 46-47 0.5345548765348411 1107 48-49 0.4971404626913696 1107 50-51 0.25777500199095726 1107 52-53 0.3827059048307646 1107 54-55 0.6015689182048192 1107 56-57 0.4563829796514085 1107 58-59 0.5229368837294572 1107 60-61 0.3324461504529026 1107 62-63 0.4754268392244754 1107 64-65 0.463222125288425 1107 66-67 0.5178764564574934 1107 68-69 0.5825041457762055 1107 70-71 0.5280158897826475 1107 72-73 0.44420009787513237 1107 74-75 0.5983866249708711 1107 76-77 0.5303251395995439 1107 78-79 0.6162591397019135 1107 80-81 0.42286156207901726 1107 82-83 0.43576260026802416 1107 84-85 0.4479853804275251 1107 86-87 0.37141302448823765 1107 88-89 0.5263552463612129 1107 90-91 0.6148637237603616 1107 92-93 0.5066616980829544 1107 94-95 0.711609242988402 1107 96-97 0.5957792873922507 1107 98-99 0.875587632574856 1107 100-101 0.9133666783792798 1108 1 0.22894711790555888 1108 2 0.24260767149955598 1108 3 0.17438651113294412 1108 4 0.19134946637386463 1108 5 0.2694833972404851 1108 6 0.23636296110418442 1108 7 0.2769927744162288 1108 8 0.3019439774293602 1108 9 0.4451450595066788 1108 10-11 0.31288003240959483 1108 12-13 0.2867147266417831 1108 14-15 0.4068421993573068 1108 16-17 0.4315751660785523 1108 18-19 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-1.3437105734573898 2316 96-97 -1.340363597842309 2316 98-99 -1.296178770705886 2316 100-101 -0.8036029881780493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5993.0 3 1591.0 4 1648.0 5 1781.0 6 2123.0 7 2682.0 8 3346.0 9 4185.0 10 5209.0 11 6987.0 12 9492.0 13 13882.0 14 13532.0 15 15569.0 16 21431.0 17 28585.0 18 22381.0 19 14305.0 20 14119.0 21 14515.0 22 15188.0 23 16735.0 24 18595.0 25 20721.0 26 23685.0 27 27375.0 28 32765.0 29 38473.0 30 45526.0 31 55427.0 32 68871.0 33 88615.0 34 120943.0 35 177793.0 36 262500.0 37 393798.0 38 367514.0 39 61174.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.9861121493121 18.951014836001402 14.7129090129397 21.3499640017468 2 15.188836210049631 21.088473447753934 40.59717085667182 23.125519485524613 3 18.859682072055485 27.02785245197324 29.852967532068988 24.25949794390229 4 12.383943797103143 16.790458313058735 37.128753950784166 33.696843939053956 5 13.300665566065067 38.734039721831294 32.42834877351014 15.536945938593503 6 30.42857212956284 36.24938049883951 17.91724936577802 15.40479800581963 7 28.037816126079928 34.536389310785644 20.230204317987198 17.195590245147233 8 27.22706924253979 33.79915766867974 19.763524953402843 19.210248135377622 9 28.540371725903917 16.43793264884887 20.099934338958196 34.92176128628901 10-11 25.26412461573454 27.23478782314305 26.166659264953445 21.334428296168966 12-13 26.54104500958786 25.032039288768278 27.364950993322235 21.061964708321625 14-15 23.500861950092773 25.557887567161195 26.959899814556827 23.981350668189208 16-17 21.519106917473586 29.22112910222613 27.187363210056354 22.07240077024393 18-19 21.44585272396331 26.538037042544303 31.307187329312153 20.70892290418023 20-21 22.94154093802949 25.632544791785524 30.901037851711983 20.524876418473 22-23 22.1546377913781 25.864885618136185 30.812512592324282 21.16796399816143 24-25 21.617288883347747 26.101980772459044 31.206541447788872 21.074188896404333 26-27 21.427325018486663 26.392156795373438 31.05025599089374 21.13026219524616 28-29 21.22694920936113 26.303485368383186 31.386156788813995 21.083408633441692 30-31 21.354845865896042 26.26861430228238 31.130971165837785 21.245568665983797 32-33 21.135594359915373 26.464680273003577 30.919686557268278 21.480038809812775 34-35 21.529984077771655 26.472634069439398 30.765294359677192 21.232087493111756 36-37 21.966192341596738 26.158336077301648 30.536183713477538 21.339287867624076 38-39 21.53446638487996 26.3693554498172 30.589980662018334 21.506197503284504 40-41 21.408649782594363 26.399754797535774 30.705016122160416 21.486579297709444 42-43 21.62256258842345 26.424407947345756 30.106587931352646 21.846441532878146 44-45 21.45869327537021 26.81669784899746 29.926597047591464 21.798011828040867 46-47 21.724314692193182 27.099112374705893 29.276623160255138 21.89994977284579 48-49 21.9245331498321 27.023583452205507 29.02427847296261 22.02760492499978 50-51 21.89897083748318 27.388288775830887 28.68636395964858 22.02637642703735 52-53 21.962999887040215 27.457299847688866 28.456366391338474 22.12333387393245 54-55 21.45813304382554 27.496973972596333 28.55915326844179 22.485739715136337 56-57 21.74884268342456 27.756039609416572 28.28548872500456 22.20962898215431 58-59 21.76899228314739 27.136524238276888 28.692808656885887 22.401674821689838 60-61 21.84009477060223 27.349668706129503 28.89487898455011 21.91535753871816 62-63 21.786141430911986 27.325538910317714 28.823192045781386 22.06512761298891 64-65 21.513996582993823 27.13729011032175 28.919724965950532 22.428988340733895 66-67 21.635261455764432 27.468877829791634 28.474066088771405 22.42179462567253 68-69 21.655052869464104 27.35863384428462 28.706850101401756 22.279463184849526 70-71 21.631741188384414 27.4947957012751 28.58065229179913 22.29281081854136 72-73 21.75157309866535 27.447682948169145 28.340953430569666 22.459790522595842 74-75 21.546702423109373 27.77474228681747 28.100704791776803 22.57785049829635 76-77 21.64458061102703 27.47360131901123 28.24059650806541 22.641221561896323 78-79 21.71246577061329 27.419946906007887 28.321388415274733 22.546198908104092 80-81 21.598387023361838 27.89768079251378 28.1441788544077 22.359753329716682 82-83 21.805765461267907 27.726886407804734 27.872580520542105 22.59476761038525 84-85 21.743222845241043 27.373868218477327 27.869251027259217 23.01365790902241 86-87 21.759986062868666 27.236486346827878 28.002905537549054 23.000622052754405 88-89 21.748665159885494 27.129665541830896 28.1084006331631 23.01326866512051 90-91 21.59456417151197 27.331535273488644 28.129585354140414 22.944315200858973 92-93 21.774367436635362 27.033462673370952 28.105391618726177 23.086778271267512 94-95 21.679184546715234 26.87619285789183 28.23289410400219 23.211728491390748 96-97 21.8156894059636 26.76262863962002 28.15590452409741 23.265777430318977 98-99 21.829750765091262 26.659781624715663 28.009010281782977 23.5014573284101 100-101 21.984986791772634 26.211102785477962 28.526818176784772 23.27709224596463 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3633.0 1 2285.0 2 1363.5 3 1705.0 4 1381.0 5 1331.5 6 2265.0 7 4629.5 8 7221.0 9 7679.0 10 6200.5 11 5117.5 12 5108.5 13 5329.0 14 5499.5 15 5577.0 16 5690.5 17 5823.0 18 5889.5 19 5976.5 20 6194.5 21 6748.5 22 7605.0 23 8719.5 24 9963.5 25 11325.0 26 13295.5 27 15545.5 28 17727.0 29 20811.5 30 24550.5 31 27976.0 32 31940.5 33 35837.0 34 39008.5 35 41198.0 36 45019.0 37 50358.0 38 52619.5 39 52945.5 40 53802.5 41 57453.5 42 61040.0 43 62814.0 44 65426.0 45 69479.0 46 70524.0 47 70562.0 48 76875.5 49 77694.5 50 71563.5 51 67315.5 52 64887.0 53 65009.5 54 64872.0 55 61562.5 56 54746.0 57 49540.0 58 45822.0 59 39648.0 60 34032.5 61 30344.5 62 26671.5 63 20747.0 64 15182.5 65 11941.5 66 9377.0 67 7793.5 68 6041.0 69 3994.5 70 3024.5 71 2529.5 72 2178.5 73 1900.0 74 1479.0 75 1250.0 76 930.0 77 534.0 78 336.5 79 254.5 80 183.0 81 108.0 82 72.0 83 64.5 84 49.5 85 32.0 86 22.5 87 16.5 88 15.5 89 10.5 90 7.0 91 6.5 92 4.0 93 3.5 94 3.5 95 2.5 96 3.5 97 2.5 98 2.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27640078702622967 2 0.10774530324638512 3 0.11980963852112829 4 0.12000580657437612 5 0.12780348669097844 6 0.055809811149015134 7 0.06439216347860886 8 0.06816839850363009 9 0.06522587770491226 10-11 0.06137607965992307 12-13 0.06549560877812804 14-15 0.06115539060001922 16-17 0.06238144093281833 18-19 0.06655001206433526 20-21 0.07089023024244409 22-23 0.07927641451878994 24-25 0.08739286772192001 26-27 0.08771164080844779 28-29 0.08165495216442022 30-31 0.07792775915271094 32-33 0.07057145715591633 34-35 0.23564687396398745 36-37 0.35991933569650447 38-39 0.3333876064947319 40-41 0.316909490021912 42-43 0.3404986984249667 44-45 0.3531760588661094 46-47 0.35793313415736994 48-49 0.36654000749361965 50-51 0.36131703307589547 52-53 0.3615132011291433 54-55 0.34886036169465656 56-57 0.3272328338240804 58-59 0.31259379285045913 60-61 0.2710061655619136 62-63 0.33782590869946466 64-65 0.36609862937381193 66-67 0.3706595366118246 68-69 0.38211084672016826 70-71 0.3832388130263434 72-73 0.3672511166866431 74-75 0.37007103245208106 76-77 0.3552113024185559 78-79 0.334613656827531 80-81 0.33351021152801186 82-83 0.3467760761288981 84-85 0.3533477059127013 86-87 0.3475117063285776 88-89 0.3611453860293036 90-91 0.38274839289322377 92-93 0.3790947629014824 94-95 0.3779667965953073 96-97 0.3831162079930635 98-99 0.37325876331735874 100-101 0.3526365967196778 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2039068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.121226101717454 #Duplication Level Percentage of deduplicated Percentage of total 1 67.91779020136275 27.24945016997885 2 14.624144206729392 11.734771925246228 3 6.163904777836905 7.419102517831528 4 3.348796755327587 5.374313271567837 5 2.047523413676621 4.107457491434004 6 1.284595509721243 3.092372813686618 7 0.8839833462469266 2.482654699344801 8 0.6332868022152681 2.0326594383129915 9 0.45490569874551073 1.6426236954895566 >10 2.34255711470128 16.918113125699087 >50 0.1836075760426034 5.117187038647602 >100 0.10716329324425439 8.15539807128724 >500 0.005406625120493147 1.4439505550553149 >1k 0.0019660454983611445 1.346484262339812 >5k 1.2287784364757153E-4 0.45444829855918234 >10k+ 2.4575568729514306E-4 1.4290126255192512 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17371 0.8519088132421283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11615 0.569622984618463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9218 0.452069278709685 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3689 0.18091598710783555 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2552 0.12515521797213236 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2522 0.12368395757277344 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.9042013311964096E-5 0.0 10-11 0.0 0.0 0.0 4.9042013311964096E-5 0.0 12-13 0.0 0.0 0.0 4.9042013311964096E-5 0.0 14-15 0.0 0.0 0.0 4.9042013311964096E-5 0.0 16-17 0.0 0.0 0.0 7.356301996794615E-5 0.0 18-19 0.0 0.0 0.0 9.808402662392819E-5 0.0 20-21 0.0 0.0 0.0 3.187730865277666E-4 0.0 22-23 0.0 0.0 0.0 6.865881863674973E-4 0.0 24-25 0.0 0.0 0.0 0.0016429074459507972 0.0 26-27 0.0 0.0 0.0 0.002648268718846061 0.0 28-29 0.0 0.0 0.0 0.0063509407238993495 0.0 30-31 0.0 0.0 0.0 0.017557040765683145 0.0 32-33 0.0 0.0 0.0 0.034991476498086385 0.0 34-35 0.0 0.0 0.0 0.058605205907797095 0.0 36-37 0.0 0.0 0.0 0.09818211065055212 0.0 38-39 0.0 0.0 0.0 0.15872447608417178 0.0 40-41 0.0 0.0 0.0 0.23049746256623124 0.0 42-43 0.0 0.0 0.0 0.3030796422679381 0.0 44-45 0.0 0.0 0.0 0.3876771152310762 0.0 46-47 0.0 0.0 0.0 0.4878944694340748 0.0 48-49 0.0 0.0 0.0 0.6019907134043592 0.0 50-51 0.0 0.0 0.0 0.741686888323489 0.0 52-53 0.0 0.0 0.0 0.8775087441909735 0.0 54-55 0.0 0.0 0.0 1.0157827007240563 0.0 56-57 0.0 0.0 0.0 1.1771064035137622 0.0 58-59 0.0 0.0 0.0 1.3714599022690759 0.0 60-61 0.0 0.0 0.0 1.5923206092195061 0.0 62-63 0.0 0.0 0.0 1.8153882067689748 0.0 64-65 0.0 0.0 0.0 2.0431393165897362 0.0 66-67 0.0 0.0 0.0 2.300315634397676 0.0 68-69 0.0 4.9042013311964096E-5 0.0 2.5989079324475695 0.0 70-71 0.0 4.9042013311964096E-5 0.0 2.937101656246874 0.0 72-73 0.0 4.9042013311964096E-5 0.0 3.2882179505538804 0.0 74-75 0.0 4.9042013311964096E-5 0.0 3.6432821269325 0.0 76-77 0.0 4.9042013311964096E-5 0.0 4.019556974068545 0.0 78-79 0.0 4.9042013311964096E-5 0.0 4.451004086180549 0.0 80-81 0.0 4.9042013311964096E-5 0.0 4.936103160855842 0.0 82-83 0.0 4.9042013311964096E-5 0.0 5.435105646305077 0.0 84-85 0.0 4.9042013311964096E-5 0.0 5.9383257448991404 2.4521006655982048E-5 86-87 0.0 4.9042013311964096E-5 0.0 6.456650783593289 4.9042013311964096E-5 88-89 0.0 4.9042013311964096E-5 0.0 7.016391802529391 4.9042013311964096E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9770 0.0 39.287 1 GTATCAA 16035 0.0 38.364796 1 ATCAACG 20655 0.0 29.83081 3 TATCAAC 20690 0.0 29.688646 2 TCAACGC 20980 0.0 29.391312 4 GCGTATC 65 0.0058425874 29.233358 1 CAACGCA 21255 0.0 29.033358 5 AACGCAG 21840 0.0 28.341307 6 GTACATG 17105 0.0 25.689207 1 TACATGG 17030 0.0 25.457317 2 ACGCAGA 24600 0.0 25.084486 7 CGCAGAG 24795 0.0 24.829866 8 ACATGGG 17460 0.0 23.93386 3 GAGTACT 12035 0.0 23.747133 12-13 GCAGAGT 25550 0.0 23.650372 9 AGTACTT 12330 0.0 23.082876 12-13 ATGGGAT 4050 0.0 22.6039 5 CATGGGA 11195 0.0 22.201805 4 GTACTTT 12880 0.0 22.06175 14-15 GTATAGG 455 0.0 21.925018 1 >>END_MODULE