FastQCFastQC Report
Fri 27 May 2016
SRR939356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939356_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039068
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175700.8616681738912091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127360.6245990815411747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97640.47884621797801735No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40160.1969527254608478No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29010.14227088061800783No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27170.13324715016860644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99200.042.102911
GTATCAA157200.036.5547371
ATCAACG208350.027.5604883
TATCAAC211400.027.2302022
TCAACGC212450.027.0509434
CAACGCA215650.026.6055285
AACGCAG220250.026.1360476
GTACATG165650.025.4719071
TACATGG166700.024.9950472
ACATGGG168350.023.8480173
ACGCAGA247300.023.2196837
CGCAGAG247950.023.0631168
AGTACTT132300.022.90375712-13
GAGTACT130250.022.86344512-13
CATGGGA109750.021.7499624
GCAGAGT263150.021.641319
GTACTTT137750.021.54970714-15
ATGGGAG40050.021.2102385
ATGGGAT39250.020.796195
ATAGCGC1404.8836396E-420.3384693
Produced by FastQC (version 0.11.5)