##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939356_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2039068 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2510779434526 33.0 31.0 34.0 31.0 34.0 2 32.43243972246144 34.0 31.0 34.0 31.0 34.0 3 32.50754315206751 34.0 31.0 34.0 30.0 34.0 4 36.07232912291302 37.0 35.0 37.0 35.0 37.0 5 36.016757655948695 37.0 35.0 37.0 35.0 37.0 6 36.06904919306271 37.0 35.0 37.0 35.0 37.0 7 36.00980791224226 37.0 35.0 37.0 35.0 37.0 8 35.99309880788674 37.0 35.0 37.0 35.0 37.0 9 37.67743204248215 39.0 37.0 39.0 35.0 39.0 10-11 37.59792709218133 39.0 37.0 39.0 35.0 39.0 12-13 37.508768466770114 39.0 37.0 39.0 35.0 39.0 14-15 38.74385062195081 40.0 38.0 41.0 34.0 41.0 16-17 38.71131958326059 40.0 38.0 41.0 34.0 41.0 18-19 38.866699394036885 40.0 38.0 41.0 35.0 41.0 20-21 38.88316966378757 40.0 38.0 41.0 35.0 41.0 22-23 38.81049602073104 40.0 38.0 41.0 34.5 41.0 24-25 38.72634066151791 40.0 38.0 41.0 34.0 41.0 26-27 38.542156269432894 40.0 38.0 41.0 34.0 41.0 28-29 38.36870006296995 40.0 38.0 41.0 34.0 41.0 30-31 38.102636841929744 40.0 37.5 41.0 33.5 41.0 32-33 37.82392078145506 40.0 36.5 41.0 33.0 41.0 34-35 37.71012712670691 40.0 37.0 41.0 33.0 41.0 36-37 37.576814750660596 40.0 36.0 41.0 33.0 41.0 38-39 37.42784031724298 40.0 36.0 41.0 33.0 41.0 40-41 37.19429219623868 40.0 36.0 41.0 32.0 41.0 42-43 36.935431530483534 40.0 35.5 41.0 31.0 41.0 44-45 36.579852658175206 40.0 35.0 41.0 30.5 41.0 46-47 36.12260282638931 39.5 35.0 41.0 29.5 41.0 48-49 35.680591574189776 39.0 35.0 41.0 27.5 41.0 50-51 35.37988777225674 39.0 35.0 41.0 27.0 41.0 52-53 35.12128359623122 39.0 35.0 40.5 26.0 41.0 54-55 34.82429644327703 38.0 35.0 40.0 25.5 41.0 56-57 34.56981498410058 38.0 34.0 40.0 24.5 41.0 58-59 34.26295003403516 38.0 34.0 40.0 24.0 41.0 60-61 33.91998329629027 37.0 34.0 40.0 23.0 41.0 62-63 33.531511945653605 37.0 33.0 40.0 22.0 41.0 64-65 33.114754632998995 36.0 33.0 39.0 21.5 41.0 66-67 32.74111579407847 36.0 33.0 39.0 20.0 40.0 68-69 32.35714919757458 35.0 32.0 39.0 18.5 40.0 70-71 31.97695393189438 35.0 32.0 38.0 16.5 40.0 72-73 31.4945693326559 35.0 31.0 37.0 12.0 39.5 74-75 31.013563794831757 35.0 31.0 37.0 7.5 39.0 76-77 29.055576125955582 33.0 28.5 35.0 4.5 37.0 78-79 30.014721431555984 34.0 30.0 36.0 2.0 38.0 80-81 30.058558615995153 34.0 30.0 35.0 2.0 37.0 82-83 29.75193863078622 34.0 30.0 35.0 2.0 37.0 84-85 29.383966596503893 34.0 30.0 35.0 2.0 36.0 86-87 28.85750622343149 34.0 29.0 35.0 2.0 36.0 88-89 28.36499469365416 34.0 29.0 35.0 2.0 35.5 90-91 27.95119706650293 34.0 28.0 35.0 2.0 35.0 92-93 27.602272704980905 33.5 27.0 35.0 2.0 35.0 94-95 27.210917683961497 33.0 26.5 35.0 2.0 35.0 96-97 26.67196974303947 33.0 25.5 35.0 2.0 35.0 98-99 25.948738590375605 33.0 24.5 35.0 2.0 35.0 100-101 22.716375569622983 29.0 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34502069975577854 1101 2 0.28479837118042894 1101 3 0.2972359045922204 1101 4 0.2268477435494276 1101 5 0.2739339409767041 1101 6 0.2744413239607013 1101 7 0.2759274554285014 1101 8 0.286440186089294 1101 9 0.27943443479626495 1101 10-11 0.3835508326474226 1101 12-13 0.4205626607943316 1101 14-15 0.44611238147160037 1101 16-17 0.3657245746459665 1101 18-19 0.3656628720479276 1101 20-21 0.31977686959759666 1101 22-23 0.3710005902474265 1101 24-25 0.29932992161553074 1101 26-27 0.3425137862463856 1101 28-29 0.43864620576354696 1101 30-31 0.34095879931027895 1101 32-33 0.3531104694495042 1101 34-35 0.4367949657995709 1101 36-37 0.43144563492758436 1101 38-39 0.5809483142336376 1101 40-41 0.6175367387522854 1101 42-43 0.6626057237694454 1101 44-45 0.5851403066246235 1101 46-47 0.508113143151661 1101 48-49 0.5170180193576712 1101 50-51 0.5882278243657737 1101 52-53 0.4948724076877795 1101 54-55 0.294321913260724 1101 56-57 0.2054059608996397 1101 58-59 0.16716446894017878 1101 60-61 0.2948379665362708 1101 62-63 0.2696969827702205 1101 64-65 0.07429039598083875 1101 66-67 0.049449416015882264 1101 68-69 0.34367376906186564 1101 70-71 0.27153056673657616 1101 72-73 0.11430663730887147 1101 74-75 0.08394615171536302 1101 76-77 0.27249427202406196 1101 78-79 0.4630815065841283 1101 80-81 0.5754810289474328 1101 82-83 0.572840886489324 1101 84-85 0.5232259259932341 1101 86-87 0.5027407490299396 1101 88-89 0.5697903231629162 1101 90-91 0.5947197126357473 1101 92-93 0.5728079122424639 1101 94-95 0.6996598945386729 1101 96-97 0.828993705958819 1101 98-99 0.7826394013956737 1101 100-101 0.5592621553248982 1102 1 0.16150304077198996 1102 2 0.22465802106808752 1102 3 0.22060854239271066 1102 4 0.15130562683804527 1102 5 0.13686198624249357 1102 6 0.12862014189948212 1102 7 0.15563772446662227 1102 8 0.14041347690550054 1102 9 0.2519532786750531 1102 10-11 0.15519603055290787 1102 12-13 0.18785208502254847 1102 14-15 0.29477373038347565 1102 16-17 0.3353109201713167 1102 18-19 0.2899460572823571 1102 20-21 0.24592303363627366 1102 22-23 0.1464049103652556 1102 24-25 0.1614115688219968 1102 26-27 0.310904026199303 1102 28-29 0.28409837321833464 1102 30-31 0.28636939423345353 1102 32-33 0.2900659588142531 1102 34-35 0.48962213150269207 1102 36-37 0.4740180871308439 1102 38-39 0.3809542309235283 1102 40-41 0.33358793991818203 1102 42-43 0.14040365635343477 1102 44-45 0.27391712441081495 1102 46-47 0.137762454713652 1102 48-49 -0.05908384735798933 1102 50-51 0.08429727384891805 1102 52-53 0.19493064037251173 1102 54-55 0.27942010695758057 1102 56-57 0.10777232545181903 1102 58-59 0.17733915142412826 1102 60-61 0.007712216634757851 1102 62-63 -0.015421140829431579 1102 64-65 0.07527708187156179 1102 66-67 0.05744457678635939 1102 68-69 0.2527432761548809 1102 70-71 0.03934377372262432 1102 72-73 0.20225558561724455 1102 74-75 0.21401897371174883 1102 76-77 0.31242087392892515 1102 78-79 0.16814370189405992 1102 80-81 0.19808340714536143 1102 82-83 0.19242372871144298 1102 84-85 0.17608236374109154 1102 86-87 0.31979187078762905 1102 88-89 0.45781198447602023 1102 90-91 0.584337478898469 1102 92-93 0.4809829092451956 1102 94-95 0.39261171555569163 1102 96-97 0.470506914969004 1102 98-99 0.5096420841474298 1102 100-101 0.4496863041389929 1103 1 0.1627023220594097 1103 2 0.1987759285821582 1103 3 0.18671890807259217 1103 4 0.16437326728116375 1103 5 0.16355073452552205 1103 6 0.1356598802470117 1103 7 0.11405152971703814 1103 8 0.13290069211441846 1103 9 0.17215918077798875 1103 10-11 0.15633369654726437 1103 12-13 0.14417081741708415 1103 14-15 0.19671230345279866 1103 16-17 0.1511486190679321 1103 18-19 0.14521188675569618 1103 20-21 0.21607600468595223 1103 22-23 0.14912378072913413 1103 24-25 0.3250671384163937 1103 26-27 0.2908312008980829 1103 28-29 0.25014697071360104 1103 30-31 0.2298441665167843 1103 32-33 0.3352365429700441 1103 34-35 0.2770340812613128 1103 36-37 0.3636712129067732 1103 38-39 0.4460149349829834 1103 40-41 0.3934744882753236 1103 42-43 0.34132959813478436 1103 44-45 0.32216465093138424 1103 46-47 0.393953186305815 1103 48-49 0.38110681451562556 1103 50-51 0.45839995336500294 1103 52-53 0.2927537093828576 1103 54-55 0.283586860262794 1103 56-57 0.37330320159377095 1103 58-59 0.1937707116640084 1103 60-61 0.1299986974749956 1103 62-63 0.25242114970608753 1103 64-65 0.3470155076757351 1103 66-67 0.41218552958201116 1103 68-69 0.22699465798867635 1103 70-71 0.2064878697238477 1103 72-73 0.2444359169148811 1103 74-75 0.29573415388526314 1103 76-77 0.32522523512970025 1103 78-79 0.3647721680983018 1103 80-81 0.2967085452064282 1103 82-83 0.40208849259185797 1103 84-85 0.34049566383784224 1103 86-87 0.17882143145071794 1103 88-89 0.47723609094724395 1103 90-91 0.6309100785317732 1103 92-93 0.35167121475430463 1103 94-95 0.3302400680086208 1103 96-97 0.07302039082987832 1103 98-99 -0.04686984200472821 1103 100-101 0.08971707203372503 1104 1 0.06875534690871632 1104 2 0.046722100686018564 1104 3 0.10315867837269366 1104 4 0.05989224223228007 1104 5 0.1360784550842311 1104 6 0.009157434280417931 1104 7 0.10268473451536408 1104 8 0.16007064105679092 1104 9 0.05103215920264148 1104 10-11 0.10869169624790231 1104 12-13 0.12333169288068291 1104 14-15 0.19403201587649477 1104 16-17 0.03449686103686389 1104 18-19 0.06621453110810904 1104 20-21 0.12038780781117708 1104 22-23 0.19408044471105512 1104 24-25 0.07698001733926674 1104 26-27 0.21591619794011052 1104 28-29 0.2574432926165784 1104 30-31 0.23822091902392373 1104 32-33 0.20462798553199946 1104 34-35 0.2240401183677747 1104 36-37 0.1834706531498682 1104 38-39 0.2725066301007857 1104 40-41 0.2889325813479857 1104 42-43 0.3444114091241701 1104 44-45 0.3492172908884328 1104 46-47 0.19465828755386383 1104 48-49 0.2862338408427334 1104 50-51 0.17332486373499023 1104 52-53 0.15036495393913185 1104 54-55 0.11946565008548049 1104 56-57 0.23615414850002026 1104 58-59 0.30930907378110106 1104 60-61 0.25334271038077105 1104 62-63 0.21157059719562454 1104 64-65 0.1975745518124299 1104 66-67 0.2369487733209823 1104 68-69 0.19281497744926668 1104 70-71 0.11861363921139301 1104 72-73 0.28907035241810064 1104 74-75 0.21940461245350917 1104 76-77 0.2186896694212237 1104 78-79 0.16754725321413844 1104 80-81 0.24266563209466696 1104 82-83 0.19925383233055882 1104 84-85 0.2114608287119104 1104 86-87 0.15921835753951896 1104 88-89 0.6499092746477686 1104 90-91 0.5421472695785141 1104 92-93 0.38355906380050797 1104 94-95 0.48056217769341103 1104 96-97 0.4322460446121532 1104 98-99 0.5353025778509632 1104 100-101 0.4461121639445693 1105 1 0.05719566181817726 1105 2 0.06947363410196061 1105 3 0.04942190304830518 1105 4 0.08994216048441928 1105 5 0.08872476315868028 1105 6 0.07186244750080562 1105 7 0.03729273951290679 1105 8 0.13987394277790344 1105 9 0.05857653130013318 1105 10-11 0.02209617138544928 1105 12-13 0.0635974048879504 1105 14-15 0.0962457868154516 1105 16-17 0.06059925580866121 1105 18-19 0.027948638328808784 1105 20-21 0.04126657727992722 1105 22-23 0.03244879404500267 1105 24-25 0.08961804193570089 1105 26-27 0.18415724028538705 1105 28-29 0.04134777404683376 1105 30-31 0.008901010874431847 1105 32-33 0.13059754718269545 1105 34-35 0.13737422970733348 1105 36-37 0.04747144717386931 1105 38-39 0.007389282735886127 1105 40-41 0.16674584612523802 1105 42-43 0.08547263249318604 1105 44-45 0.18525991647636886 1105 46-47 0.09416076401631557 1105 48-49 0.12700771676676226 1105 50-51 0.10010618334796106 1105 52-53 -0.07020753278613512 1105 54-55 0.02934221024538175 1105 56-57 0.17433440338858475 1105 58-59 0.19540839085181716 1105 60-61 0.014876652618390551 1105 62-63 0.0257438111278816 1105 64-65 0.11658810857083779 1105 66-67 0.1079544836578279 1105 68-69 0.2083988425155212 1105 70-71 0.30247224115709415 1105 72-73 0.18678741901429063 1105 74-75 0.22750703239609749 1105 76-77 0.27270017708172745 1105 78-79 0.24072503106819454 1105 80-81 0.21834435671364716 1105 82-83 0.1580910105019946 1105 84-85 0.10318753343223364 1105 86-87 0.02363475562242101 1105 88-89 0.010334100368655896 1105 90-91 0.030274951840773667 1105 92-93 0.1518224738246623 1105 94-95 0.12948486790163471 1105 96-97 0.11620704590616171 1105 98-99 0.257823512511024 1105 100-101 0.33834657493093445 1106 1 0.11641114803272501 1106 2 0.16359253100685578 1106 3 0.11391364299327478 1106 4 0.08705165353480027 1106 5 0.14951007506918756 1106 6 0.0884963443032376 1106 7 0.13429782917794597 1106 8 0.12851144624274013 1106 9 0.12965981349234568 1106 10-11 0.18560233474439514 1106 12-13 0.19305975980984869 1106 14-15 0.18241659851941705 1106 16-17 0.2963109298975368 1106 18-19 0.21807874349796208 1106 20-21 0.28679303242907395 1106 22-23 0.24241527279276198 1106 24-25 0.23024201394524368 1106 26-27 0.34587678992714643 1106 28-29 0.3240765636039029 1106 30-31 0.39124119981435257 1106 32-33 0.3509844113385796 1106 34-35 0.3793708689537283 1106 36-37 0.3796052560746901 1106 38-39 0.46985286828033423 1106 40-41 0.48093828917523496 1106 42-43 0.5227985145022629 1106 44-45 0.5992775809251825 1106 46-47 0.6539783492997557 1106 48-49 0.5534178904883831 1106 50-51 0.6825684099227232 1106 52-53 0.6474001844360018 1106 54-55 0.6467898171105517 1106 56-57 0.7171327808219274 1106 58-59 0.7717619947639278 1106 60-61 0.6902837772865951 1106 62-63 0.5470882635655627 1106 64-65 0.690960151328774 1106 66-67 0.6306732750807882 1106 68-69 0.6928949937192357 1106 70-71 0.725024720575707 1106 72-73 0.733407089776918 1106 74-75 0.5455339809806645 1106 76-77 0.6334920681311864 1106 78-79 0.7874463549203732 1106 80-81 0.7575972793073795 1106 82-83 0.7098709911528509 1106 84-85 0.8358183369608092 1106 86-87 0.7911654645623933 1106 88-89 0.911256581933582 1106 90-91 0.8747637904933221 1106 92-93 0.9228773362936948 1106 94-95 0.9441294919361987 1106 96-97 1.0329706919571144 1106 98-99 1.0604763076204584 1106 100-101 0.7022134045301591 1107 1 -0.07066879918706803 1107 2 -0.0142775984362018 1107 3 -0.05232808954588819 1107 4 0.036129438574647565 1107 5 -0.015416776657062314 1107 6 -0.03469550189424808 1107 7 -0.01983302381437113 1107 8 0.01900638402867827 1107 9 0.026109994975698214 1107 10-11 -0.034934109908320465 1107 12-13 -0.0033926311254433017 1107 14-15 0.057201305412498016 1107 16-17 0.001833634842185461 1107 18-19 0.058861012800356605 1107 20-21 -0.051790318374344224 1107 22-23 0.05079721406861637 1107 24-25 -0.014761541730891281 1107 26-27 -0.03145520951380831 1107 28-29 -0.07823328993512035 1107 30-31 0.019557176104363805 1107 32-33 -0.013525573328855955 1107 34-35 -0.12047883693225003 1107 36-37 -0.1774011729166034 1107 38-39 -0.11579576514795775 1107 40-41 -0.18676990285593575 1107 42-43 -0.2533714159873526 1107 44-45 -0.20934431710706747 1107 46-47 -0.2313192381842626 1107 48-49 -0.0010284003042642098 1107 50-51 -0.026459616204306258 1107 52-53 -0.0870488707406949 1107 54-55 -0.15828950735208736 1107 56-57 -0.04042611391773221 1107 58-59 0.014437404694334077 1107 60-61 -0.08906519485581299 1107 62-63 -0.09760257758820501 1107 64-65 0.04214174566928364 1107 66-67 0.022486690461853698 1107 68-69 0.01836473347663059 1107 70-71 0.006410756017377395 1107 72-73 0.010088790993250996 1107 74-75 0.0013696059780983205 1107 76-77 0.11645745064383561 1107 78-79 0.35676585400165095 1107 80-81 0.24494020268975092 1107 82-83 0.3737997358141989 1107 84-85 0.3067221672255762 1107 86-87 0.23322284454291164 1107 88-89 0.6500163805959929 1107 90-91 0.6511991121278662 1107 92-93 0.32671196857923235 1107 94-95 0.49692485969176303 1107 96-97 0.49742369331772807 1107 98-99 0.4782772452501973 1107 100-101 0.5320822658505691 1108 1 0.020204828789992746 1108 2 0.03589144153981749 1108 3 -0.02986180324398191 1108 4 -0.021543666103085002 1108 5 0.038355901797729075 1108 6 0.03777083403771542 1108 7 0.012228241513867033 1108 8 0.04135256559131051 1108 9 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-0.9243746703907121 2316 50-51 -0.6880725449611873 2316 52-53 -0.8764659865831845 2316 54-55 -0.6257570002411583 2316 56-57 -0.601993594730871 2316 58-59 -0.5947121149608847 2316 60-61 -0.4958388763350783 2316 62-63 -0.6090222913885484 2316 64-65 -0.7645698630573676 2316 66-67 -0.6574465736796355 2316 68-69 -0.618582894499724 2316 70-71 -0.5086721273241999 2316 72-73 -0.5381981193461982 2316 74-75 -0.45129599343703575 2316 76-77 -0.43010007160281205 2316 78-79 -0.5491272225711938 2316 80-81 -0.43193776835737907 2316 82-83 -0.4071027240863536 2316 84-85 -0.672051827575249 2316 86-87 -0.6237097619497014 2316 88-89 -0.7489636312841164 2316 90-91 -0.9480703754118913 2316 92-93 -0.9702569921886628 2316 94-95 -0.7268657721570442 2316 96-97 -0.638926461466383 2316 98-99 -0.6669606547447415 2316 100-101 0.02227026286095679 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 25.0 5 79.0 6 164.0 7 359.0 8 866.0 9 1579.0 10 2701.0 11 4286.0 12 6132.0 13 8968.0 14 10907.0 15 14587.0 16 20027.0 17 28266.0 18 25664.0 19 15166.0 20 13034.0 21 13184.0 22 13760.0 23 14709.0 24 16625.0 25 19008.0 26 22386.0 27 27285.0 28 32440.0 29 39036.0 30 47493.0 31 57635.0 32 71585.0 33 91011.0 34 124924.0 35 182328.0 36 276531.0 37 436424.0 38 367705.0 39 32175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.903426123354606 19.718502403675934 15.282569612185966 21.095501860783497 2 15.544258455333516 21.65660978447016 39.79102217287506 23.008109587321265 3 18.90795206437451 27.11272993347941 30.203014318306202 23.77630368383987 4 12.674859298463808 17.40378447408326 36.73207563455461 33.18928059289832 5 13.178913111284174 38.88246983425761 32.52873371559948 15.409883338858736 6 30.411786168975237 36.646301153271985 17.50750833223806 15.434404345514716 7 27.800397044139775 34.81468004009675 20.037291546922418 17.34763136884106 8 27.14911910735689 34.29689446354903 19.41494839799359 19.139038031100483 9 28.217008947224908 16.485865110923225 19.97044728277821 35.32667865907366 10-11 25.209630085901992 27.51487444263752 25.90688491016484 21.36861056129565 12-13 26.516158362546026 25.131334511649438 27.350706303075718 21.001800822728818 14-15 23.31950638887227 25.73403125236021 27.014503088257364 23.93195927051016 16-17 21.265460555353936 29.319318705678103 27.357189096936825 22.058031642031132 18-19 21.287789323357533 26.497252666414262 31.543136374068936 20.671821636159265 20-21 22.7711140579912 25.550815372513323 31.225785505927217 20.452285063568258 22-23 21.98970313888502 25.840702713200344 31.156243931050852 21.01335021686378 24-25 21.501794693357304 26.135016652223786 31.506934298872963 20.856254355545943 26-27 21.311457320293844 26.42362546633533 31.35504652441324 20.909870688957582 28-29 21.104960697730533 26.291742109630476 31.669983541500336 20.933313651138658 30-31 21.266603173606764 26.171834387082725 31.520405400898845 21.04115703841167 32-33 21.053981524892745 26.385020018949835 31.20790969207501 21.353088764082415 34-35 21.440333224373973 26.3727837334872 31.11077488742569 21.07610815471314 36-37 21.859198869823704 26.116907515868576 30.890872256722684 21.133021357585033 38-39 21.3477053916063 26.351678102515557 30.935082960993864 21.365533544884276 40-41 21.31464044109117 26.275003108642075 31.047719066068268 21.362637384198486 42-43 21.524286053396164 26.324236812316943 30.43326754777464 21.71820958651226 44-45 21.405892833585003 26.701243175140615 30.183447904492922 21.70941608678146 46-47 21.65942314709102 27.063275110540914 29.463567597682072 21.81373414468599 48-49 21.890760877028132 26.94326525647992 29.216411615502768 21.949562250989178 50-51 21.846990880147203 27.335650404988947 28.790211998815145 22.027146716048705 52-53 21.959762989758065 27.47130061380984 28.481271835956427 22.087664560475666 54-55 21.442860169449965 27.45749528706252 28.569939796024457 22.529704747463057 56-57 21.74935356480352 27.701216364833435 28.29585092205138 22.253579148311665 58-59 21.75670042421716 27.176160466884085 28.66192589686374 22.405213212035015 60-61 21.842219224048424 27.406340990446875 28.81988838750399 21.931551398000707 62-63 21.732220712789903 27.396237426619518 28.768164274189196 22.103377586401376 64-65 21.461692302561758 27.16964318992795 28.82736132389896 22.541303183611337 66-67 21.579049840417287 27.503545737562458 28.425118730714228 22.492285691306027 68-69 21.62427454246029 27.370628986522206 28.61723403650117 22.387862434516332 70-71 21.541581742246947 27.446313708027393 28.539975125890848 22.47212942383481 72-73 21.664554590626697 27.48272740291153 28.24177516394745 22.610942842514326 74-75 21.503574181930176 27.844951713233694 27.90007493619634 22.75139916863979 76-77 21.466339707914774 27.528414516319717 28.219412688421368 22.785833087344145 78-79 21.523975659467954 27.49621395657232 28.259209599679853 22.720600784279878 80-81 21.38280590031841 27.998558146777224 28.077271562510347 22.54136439039402 82-83 21.64194622248988 27.768274525420438 27.814619228000243 22.775160024089438 84-85 21.486311393244364 27.47036881555691 27.805620018557498 23.237699772641225 86-87 21.44619899577219 27.292379729286576 27.96692960390537 23.294491671035864 88-89 21.38475690515553 27.255744842327832 28.02288044122909 23.336617811287546 90-91 21.27834874969823 27.50489167201299 27.99618755966166 23.220572018627113 92-93 21.32819994232659 27.208778716550896 27.983054022720182 23.479967318402327 94-95 21.166273594737202 27.030156439194986 28.104422267635147 23.699147698432665 96-97 21.284895844572127 27.00765741995853 28.043351178087246 23.664095557382097 98-99 21.155915349561663 26.974088161846492 27.884258886903236 23.985737601688616 100-101 21.088522615618473 26.702071756855407 28.41714578705501 23.792259840471118 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2532.0 1 1729.5 2 1305.5 3 1651.0 4 1370.0 5 1291.0 6 2188.5 7 4471.5 8 6904.0 9 7444.5 10 6149.5 11 4951.5 12 4838.5 13 5067.5 14 5355.5 15 5507.5 16 5466.0 17 5556.5 18 5770.0 19 5917.5 20 6147.0 21 6737.0 22 7551.0 23 8680.0 24 10002.0 25 11275.0 26 13377.5 27 15803.5 28 17890.0 29 21094.5 30 24897.5 31 28265.0 32 32129.0 33 35916.0 34 39058.5 35 41227.5 36 45303.5 37 50625.0 38 52840.5 39 53233.5 40 54133.5 41 58102.5 42 61596.0 43 63648.0 44 66638.5 45 70680.5 46 71555.0 47 71018.0 48 77266.0 49 78145.0 50 72333.0 51 67971.0 52 64730.0 53 64764.5 54 64894.0 55 61491.5 56 54430.5 57 48681.5 58 44940.5 59 39423.5 60 33607.5 61 29699.5 62 26284.5 63 20262.0 64 14773.0 65 11701.0 66 8944.0 67 7444.5 68 5833.5 69 3762.0 70 2790.5 71 2372.0 72 2058.5 73 1827.5 74 1492.5 75 1303.0 76 946.5 77 493.0 78 283.5 79 170.5 80 115.0 81 78.5 82 48.5 83 35.0 84 21.5 85 9.0 86 4.0 87 2.5 88 2.0 89 1.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2705157454287939 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002648268718846061 16-17 3.9233610649571277E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.9042013311964096E-5 26-27 0.001520302412670887 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003996924084925074 36-37 0.021970821963759914 38-39 0.007748638103290326 40-41 0.019445158278193764 42-43 0.005885041597435691 44-45 7.356301996794614E-4 46-47 1.9616805324785638E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.4521006655982048E-5 58-59 8.827562396153537E-4 60-61 0.0024030586522862407 62-63 0.0018635965058546354 64-65 0.0 66-67 0.0 68-69 4.904201331196409E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23366067242485294 78-79 0.0 80-81 0.0012505713394550843 82-83 0.0 84-85 0.0 86-87 0.10200738768888533 88-89 0.35746723503090627 90-91 0.35793313415736994 92-93 0.0 94-95 4.9042013311964096E-5 96-97 0.0 98-99 0.0 100-101 7.356301996794615E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2039068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.72592528245894 #Duplication Level Percentage of deduplicated Percentage of total 1 66.56343810492199 25.77730730594789 2 15.186201708526461 11.76199425377492 3 6.470151413452456 7.516878006106678 4 3.4104011330488126 5.282837578466465 5 2.1205892907663126 4.106089121449941 6 1.3564896373471176 3.151878980540058 7 0.9452408437112046 2.5623728401241994 8 0.6354973684713336 1.9688178886896117 9 0.4914146789817643 1.7127439326856218 >10 2.4981077582197493 17.374006396902914 >50 0.1969481195796591 5.261745804921402 >100 0.11751701224781352 8.68083846687185 >500 0.005589348443028729 1.4745839518673194 >1k 0.002032490342919538 1.3967101771948933 >5k 1.2703064643247112E-4 0.48032819703194346 >10k+ 2.5406129286494224E-4 1.4908670974242195 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17570 0.8616681738912091 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12736 0.6245990815411747 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9764 0.47884621797801735 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4016 0.1969527254608478 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2901 0.14227088061800783 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2717 0.13324715016860644 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.4521006655982048E-5 0.0 18-19 0.0 0.0 0.0 4.9042013311964096E-5 0.0 20-21 0.0 0.0 0.0 2.697310732158025E-4 0.0 22-23 0.0 0.0 0.0 6.375461730555331E-4 0.0 24-25 0.0 0.0 0.0 0.0016183864392948152 0.0 26-27 0.0 0.0 0.0 0.002648268718846061 0.0 28-29 0.0 0.0 0.0 0.006424503743867296 0.0 30-31 0.0 0.0 0.0 0.017704166805619037 0.0 32-33 0.0 0.0 0.0 0.03538381260458209 0.0 34-35 0.0 0.0 0.0 0.059169189060884676 0.0 36-37 0.0 0.0 0.0 0.09935911897003925 0.0 38-39 0.0 0.0 0.0 0.16066163560999436 0.0 40-41 0.0 0.0 0.0 0.23346450437160504 0.0 42-43 0.0 0.0 0.0 0.30665970923971153 0.0 44-45 0.0 0.0 0.0 0.39245871152899264 0.0 46-47 0.0 0.0 0.0 0.4940737631113823 0.0 48-49 0.0 0.0 0.0 0.60924893137453 0.0 50-51 0.0 0.0 0.0 0.7500485515931788 0.0 52-53 0.0 0.0 0.0 0.8873416678600223 0.0 54-55 0.0 0.0 0.0 1.0270378427791522 0.0 56-57 0.0 0.0 0.0 1.189415948855065 0.0 58-59 0.0 0.0 0.0 1.3865158003558489 0.0 60-61 0.0 0.0 0.0 1.6091910617988219 0.0 62-63 0.0 0.0 0.0 1.8334847096810896 0.0 64-65 0.0 0.0 0.0 2.062486390841306 0.0 66-67 0.0 0.0 0.0 2.322065767301532 0.0 68-69 0.0 0.0 0.0 2.6221048047441284 0.0 70-71 0.0 0.0 0.0 2.9628241922289984 0.0 72-73 0.0 0.0 0.0 3.3170301333746592 0.0 74-75 0.0 0.0 0.0 3.6739088642458224 0.0 76-77 0.0 0.0 0.0 4.052660333054121 0.0 78-79 0.0 0.0 0.0 4.486240772745195 0.0 80-81 0.0 0.0 0.0 4.974306889225862 0.0 82-83 0.0 0.0 0.0 5.4756388703074155 0.0 84-85 0.0 0.0 0.0 5.981580800640293 0.0 86-87 0.0 0.0 0.0 6.504074410465958 0.0 88-89 0.0 0.0 0.0 7.067518101407114 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9920 0.0 42.10291 1 GTATCAA 15720 0.0 36.554737 1 ATCAACG 20835 0.0 27.560488 3 TATCAAC 21140 0.0 27.230202 2 TCAACGC 21245 0.0 27.050943 4 CAACGCA 21565 0.0 26.605528 5 AACGCAG 22025 0.0 26.136047 6 GTACATG 16565 0.0 25.471907 1 TACATGG 16670 0.0 24.995047 2 ACATGGG 16835 0.0 23.848017 3 ACGCAGA 24730 0.0 23.219683 7 CGCAGAG 24795 0.0 23.063116 8 AGTACTT 13230 0.0 22.903757 12-13 GAGTACT 13025 0.0 22.863445 12-13 CATGGGA 10975 0.0 21.749962 4 GCAGAGT 26315 0.0 21.64131 9 GTACTTT 13775 0.0 21.549707 14-15 ATGGGAG 4005 0.0 21.210238 5 ATGGGAT 3925 0.0 20.79619 5 ATAGCGC 140 4.8836396E-4 20.338469 3 >>END_MODULE