##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939355_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 365835 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.799513441852202 33.0 31.0 34.0 30.0 34.0 2 31.74620525646808 33.0 31.0 34.0 30.0 34.0 3 31.72672652971968 33.0 31.0 34.0 30.0 34.0 4 35.252955567400605 37.0 35.0 37.0 33.0 37.0 5 35.34567222928369 37.0 35.0 37.0 33.0 37.0 6 35.516935230363416 37.0 35.0 37.0 33.0 37.0 7 35.49260458950073 37.0 35.0 37.0 33.0 37.0 8 35.49539819864147 37.0 35.0 37.0 33.0 37.0 9 37.009214536608035 39.0 37.0 39.0 33.0 39.0 10-11 36.95780064783304 39.0 37.0 39.0 33.0 39.0 12-13 36.857560102231886 39.0 37.0 39.0 33.0 39.0 14-15 37.81131521040906 40.0 38.0 41.0 33.0 41.0 16-17 37.79442098213676 40.0 37.5 41.0 32.5 41.0 18-19 37.9472139079093 40.0 38.0 41.0 33.5 41.0 20-21 37.967268030669565 40.0 38.0 41.0 33.0 41.0 22-23 37.90738857681742 40.0 38.0 41.0 33.0 41.0 24-25 37.76212636844479 40.0 37.0 41.0 33.0 41.0 26-27 37.50117539327839 40.0 36.5 41.0 33.0 41.0 28-29 37.23083493924857 39.5 36.0 41.0 32.5 41.0 30-31 36.83424904670138 39.0 35.0 41.0 32.0 41.0 32-33 36.332466822474615 38.0 35.0 41.0 31.5 41.0 34-35 35.928598958546885 38.0 35.0 41.0 30.5 41.0 36-37 35.50932797572676 38.0 35.0 40.0 30.0 41.0 38-39 34.732347916410404 37.5 34.5 40.0 27.0 41.0 40-41 34.520089657905885 37.0 35.0 40.0 25.0 41.0 42-43 34.35995599108888 38.0 35.0 40.0 23.5 41.0 44-45 33.938983421487826 38.0 35.0 40.0 19.5 41.0 46-47 33.26203753058073 38.0 33.5 40.0 5.5 41.0 48-49 32.73873877567756 38.0 33.0 40.0 2.0 41.0 50-51 31.50419314718384 36.5 31.5 39.0 2.0 40.5 52-53 31.521084641983407 36.5 32.0 39.5 2.0 40.5 54-55 31.90723276887121 37.0 33.0 40.0 2.0 41.0 56-57 31.831377533587546 37.0 33.0 40.0 2.0 41.0 58-59 31.617667801057856 37.0 33.0 40.0 2.0 41.0 60-61 31.24614785354053 36.0 32.0 40.0 2.0 41.0 62-63 30.760460316809493 36.0 31.0 39.5 2.0 41.0 64-65 30.212735249497726 35.0 30.0 39.0 2.0 41.0 66-67 29.768241146965163 35.0 29.0 39.0 2.0 40.5 68-69 29.318538412125683 35.0 29.0 38.0 2.0 40.0 70-71 28.76922383041535 35.0 28.0 37.0 2.0 39.5 72-73 28.19288887066574 35.0 26.0 37.0 2.0 39.0 74-75 27.666058468981916 35.0 26.0 36.0 2.0 39.0 76-77 27.066546393866087 34.0 24.0 36.0 2.0 38.0 78-79 26.597792720762094 34.0 21.5 35.0 2.0 37.0 80-81 26.199192258805198 34.0 19.0 35.0 2.0 37.0 82-83 25.710056446212093 34.0 11.0 35.0 2.0 36.0 84-85 25.22857572402859 34.0 2.0 35.0 2.0 36.0 86-87 24.696878374130414 33.0 2.0 35.0 2.0 35.5 88-89 24.09279319911982 33.0 2.0 35.0 2.0 35.0 90-91 23.51843043995244 32.5 2.0 35.0 2.0 35.0 92-93 23.044934191643776 32.0 2.0 35.0 2.0 35.0 94-95 22.527907389943554 32.0 2.0 35.0 2.0 35.0 96-97 21.828305110227287 31.0 2.0 35.0 2.0 35.0 98-99 21.098821873248866 31.0 2.0 34.5 2.0 35.0 100-101 18.95521204914784 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5079096301508308 1101 2 0.3657902160947586 1101 3 0.42634303418032715 1101 4 0.011544917020927414 1101 5 0.19110346216652374 1101 6 0.21746483612423617 1101 7 0.2198713148148741 1101 8 0.3407796694604244 1101 9 0.5581722300646632 1101 10-11 0.7253694644089563 1101 12-13 0.682688097441094 1101 14-15 0.6271355394788145 1101 16-17 0.8193800260168942 1101 18-19 0.7491917179389347 1101 20-21 0.7522822140841257 1101 22-23 0.6731783520801073 1101 24-25 0.6075690435989216 1101 26-27 0.5003768689080843 1101 28-29 0.827184252053371 1101 30-31 0.560830898496711 1101 32-33 0.7107680774124461 1101 34-35 0.539382870934773 1101 36-37 0.46537404672174176 1101 38-39 0.8186143884399257 1101 40-41 0.5295479082134804 1101 42-43 0.20455901536749366 1101 44-45 0.11195162426602678 1101 46-47 0.10318628974889776 1101 48-49 0.1656106241292008 1101 50-51 0.16928004071165148 1101 52-53 0.05929617151608113 1101 54-55 -0.041736022512164794 1101 56-57 -0.03759671160454303 1101 58-59 -0.01674522828831826 1101 60-61 0.2972640638129995 1101 62-63 0.5551271746628039 1101 64-65 0.2826533806505829 1101 66-67 0.33026935506959276 1101 68-69 0.6464741954901143 1101 70-71 0.7131317597966138 1101 72-73 0.7993175044964964 1101 74-75 0.863088452461259 1101 76-77 1.1380344296675133 1101 78-79 0.6558303064733089 1101 80-81 0.9783034322844806 1101 82-83 1.098677335760474 1101 84-85 0.9461361171265033 1101 86-87 0.7797252659195131 1101 88-89 0.6030828611080779 1101 90-91 0.7066654789535995 1101 92-93 0.7004807861505462 1101 94-95 0.7709916327539652 1101 96-97 0.671476857420469 1101 98-99 0.7337129611891307 1101 100-101 0.31104177673700306 1102 1 -0.2075653317637567 1102 2 -0.053286888431262724 1102 3 0.07487613162983564 1102 4 0.11656236259565134 1102 5 -0.10601187519649358 1102 6 -0.01430085260739844 1102 7 0.05723924444286155 1102 8 0.22589441711387082 1102 9 0.026686653377851144 1102 10-11 0.04329663527730787 1102 12-13 0.07520861441981452 1102 14-15 0.16436587020758964 1102 16-17 0.17220326057387325 1102 18-19 -0.046970255622426293 1102 20-21 -0.01634523689626377 1102 22-23 0.41124410469695505 1102 24-25 -0.06749115591949106 1102 26-27 0.011959747673621735 1102 28-29 -0.12795359255815697 1102 30-31 0.0772444814737625 1102 32-33 -0.024088672304984016 1102 34-35 0.2252150985626642 1102 36-37 0.31279652448477435 1102 38-39 0.881841082626373 1102 40-41 0.5525809810917508 1102 42-43 0.5198374074260741 1102 44-45 0.7811343850896577 1102 46-47 0.9694745594427374 1102 48-49 1.0440321681854101 1102 50-51 1.0503307065920389 1102 52-53 1.0443765194447252 1102 54-55 0.87991123288122 1102 56-57 0.8710916760312166 1102 58-59 1.1569488107307926 1102 60-61 0.7172011614590765 1102 62-63 0.8769857426514029 1102 64-65 0.8912582260975306 1102 66-67 0.41091705274515533 1102 68-69 0.5731831246758254 1102 70-71 0.7075688328714556 1102 72-73 0.5878771388765642 1102 74-75 0.7023090529821694 1102 76-77 0.5842037745984712 1102 78-79 0.9002010372217484 1102 80-81 0.7711482895256232 1102 82-83 0.6568079564297946 1102 84-85 0.7916764385182198 1102 86-87 0.39185854028189127 1102 88-89 0.10396742546013371 1102 90-91 0.01701979611906168 1102 92-93 0.13686095225207495 1102 94-95 0.3465678281443978 1102 96-97 0.3950210119243742 1102 98-99 0.24395916493378778 1102 100-101 0.0053903933766434875 1103 1 0.2669636698680442 1103 2 0.3790228578971764 1103 3 0.3432339420437174 1103 4 0.17204374517983467 1103 5 0.30980180229942533 1103 6 0.4682388299322824 1103 7 0.6106586184229883 1103 8 0.4488880382383371 1103 9 0.4215538837590955 1103 10-11 0.5490821270441018 1103 12-13 0.47602451710488225 1103 14-15 0.6082317654753524 1103 16-17 0.622448031379399 1103 18-19 0.7910211403128926 1103 20-21 0.9546828004869141 1103 22-23 0.868653984536742 1103 24-25 0.8095597267388754 1103 26-27 0.8509598960195603 1103 28-29 0.6719128960933745 1103 30-31 0.7299666295230054 1103 32-33 0.7703631894365941 1103 34-35 0.9085706837874099 1103 36-37 0.4793445143972477 1103 38-39 0.9922252986180595 1103 40-41 0.8977374860613452 1103 42-43 0.4501296489332631 1103 44-45 -0.2706086797375207 1103 46-47 -0.6620934571720127 1103 48-49 -0.5480164892837962 1103 50-51 -0.6250883706961936 1103 52-53 -0.4334605967101446 1103 54-55 -0.523574896773134 1103 56-57 -0.6398265853543386 1103 58-59 -0.7712517529837868 1103 60-61 -0.4840597790542027 1103 62-63 -0.7167688931593084 1103 64-65 -0.7779303215956865 1103 66-67 -0.77961847610813 1103 68-69 -0.7289827260881587 1103 70-71 -0.47047596760325305 1103 72-73 -0.43869161946789603 1103 74-75 -0.4119469921149985 1103 76-77 -0.5955766734062777 1103 78-79 -0.5431753164349793 1103 80-81 -0.7648849399655511 1103 82-83 -0.7620616474865365 1103 84-85 -0.4636417635289547 1103 86-87 -0.237157926117753 1103 88-89 -0.49171570760617556 1103 90-91 -0.12451706784770877 1103 92-93 -0.05733872138685214 1103 94-95 0.6287069766386857 1103 96-97 0.3684522535799175 1103 98-99 0.5652764769053071 1103 100-101 0.3540478625948573 1104 1 0.46247435077592414 1104 2 0.655641682997306 1104 3 0.6968999411536458 1104 4 0.5610068070400942 1104 5 0.5507341565495381 1104 6 0.5158578775513334 1104 7 0.6681519428555589 1104 8 0.5518864806059369 1104 9 0.509226335917532 1104 10-11 0.5790109209915926 1104 12-13 0.7137998842610855 1104 14-15 0.958810314652041 1104 16-17 0.9582151653357798 1104 18-19 0.9492599031077305 1104 20-21 1.0169880964370677 1104 22-23 0.9927917237445669 1104 24-25 0.8889572567789301 1104 26-27 0.9698962556101307 1104 28-29 1.0815702169656447 1104 30-31 1.0246651163943952 1104 32-33 1.130871645155338 1104 34-35 0.6805722414198101 1104 36-37 0.6100187467069915 1104 38-39 1.1579323524676468 1104 40-41 1.0735135207742985 1104 42-43 0.8544604232990878 1104 44-45 0.6914518454071157 1104 46-47 0.8040975753157511 1104 48-49 0.756333755487006 1104 50-51 0.808267214528545 1104 52-53 1.1884241384923442 1104 54-55 1.3603675820875694 1104 56-57 0.9164289776185122 1104 58-59 0.9967305567625395 1104 60-61 1.065812272570188 1104 62-63 0.9831365363021938 1104 64-65 0.8069328292721387 1104 66-67 1.200897211475315 1104 68-69 1.2837982040409024 1104 70-71 1.3235410550936777 1104 72-73 1.3346033293527562 1104 74-75 1.4378249259980436 1104 76-77 1.2085093301540226 1104 78-79 0.9608161165868303 1104 80-81 0.9624181307954629 1104 82-83 1.1832960516678916 1104 84-85 1.2809621528039337 1104 86-87 1.284120445043797 1104 88-89 1.3911698064125169 1104 90-91 1.513249954849222 1104 92-93 1.7114641268552475 1104 94-95 1.9127384630650361 1104 96-97 2.0851995833529493 1104 98-99 2.163602022076649 1104 100-101 2.012731663217913 1105 1 0.1884826112229625 1105 2 0.22154304354152643 1105 3 0.23619780315558714 1105 4 0.40454422200608064 1105 5 0.2027020865786966 1105 6 0.267072649174267 1105 7 0.2652729341092055 1105 8 0.6001736526175989 1105 9 0.6588861998631117 1105 10-11 0.4728398811470811 1105 12-13 0.332513441112404 1105 14-15 0.40754695216418213 1105 16-17 0.5224462148984585 1105 18-19 0.4102049954303766 1105 20-21 0.7123233734049919 1105 22-23 0.7913218500808128 1105 24-25 0.358618335496125 1105 26-27 0.5546873148909839 1105 28-29 0.5601902364019935 1105 30-31 0.6966160396208281 1105 32-33 0.6442948715887624 1105 34-35 0.8000450305354505 1105 36-37 0.5430848302832842 1105 38-39 1.1165997479972845 1105 40-41 1.0940127919113252 1105 42-43 0.7824548353496183 1105 44-45 0.9020932448240231 1105 46-47 1.1893320262389793 1105 48-49 0.5978060586940046 1105 50-51 0.3877132786684889 1105 52-53 0.26556213654871286 1105 54-55 0.31016714476978535 1105 56-57 0.6319598328177385 1105 58-59 0.6405898862086055 1105 60-61 0.5262714562436557 1105 62-63 0.1609183633187179 1105 64-65 0.13097315969001855 1105 66-67 -0.3629086680193403 1105 68-69 -0.19545957049748353 1105 70-71 0.0845043689906646 1105 72-73 -0.11756503799418638 1105 74-75 -0.06588012745190852 1105 76-77 0.2922374642648151 1105 78-79 0.379760480046496 1105 80-81 0.42913338832704895 1105 82-83 0.03737161046283788 1105 84-85 0.23006322180296124 1105 86-87 0.23218895816333074 1105 88-89 -0.015566102236657997 1105 90-91 0.05242633968887134 1105 92-93 -0.2263395660504841 1105 94-95 0.277504012141808 1105 96-97 0.4096954531294301 1105 98-99 0.45873078786867794 1105 100-101 0.1403434222072235 1106 1 0.1332246276308524 1106 2 0.29136981145799723 1106 3 0.30895697326881333 1106 4 0.40555276495814496 1106 5 0.5004849871143264 1106 6 0.43335510900205065 1106 7 0.21071838803828769 1106 8 0.34985159688501 1106 9 0.15692844001497974 1106 10-11 0.266580134723597 1106 12-13 0.2955066948923175 1106 14-15 0.2687494065678777 1106 16-17 0.1929535374287994 1106 18-19 0.19981361296377287 1106 20-21 0.33530204992543844 1106 22-23 0.4941067846526508 1106 24-25 0.4976574228918835 1106 26-27 0.45273125007302895 1106 28-29 0.5956206045625478 1106 30-31 0.46105769668232455 1106 32-33 0.23877585335024065 1106 34-35 0.325436582505084 1106 36-37 0.44404421736037136 1106 38-39 0.46859818856952273 1106 40-41 0.09355089618957635 1106 42-43 -0.013306741706450964 1106 44-45 0.012880416835685082 1106 46-47 0.21692287985617043 1106 48-49 -0.3452027893634977 1106 50-51 -0.44069457949138524 1106 52-53 -0.341614621197575 1106 54-55 -0.26909670362671534 1106 56-57 -0.36371360266941366 1106 58-59 -0.30216894489858603 1106 60-61 -0.38310799321054745 1106 62-63 -0.18200742826037342 1106 64-65 -0.005290315799847178 1106 66-67 -0.07343141532424013 1106 68-69 -0.014201519104169336 1106 70-71 -0.10271171345560148 1106 72-73 -0.25021809921867444 1106 74-75 -0.4355831581658549 1106 76-77 0.019244379987910065 1106 78-79 0.26774441116047143 1106 80-81 0.33271159701916275 1106 82-83 0.32867949574319866 1106 84-85 0.010544101862627997 1106 86-87 -0.009068923726967881 1106 88-89 -0.014595697463455792 1106 90-91 -0.1414427361806787 1106 92-93 -0.3918719750273638 1106 94-95 -0.8472629212096052 1106 96-97 -0.980864923845278 1106 98-99 -0.690080148465988 1106 100-101 -0.8864931429836389 1107 1 0.2588919283610487 1107 2 0.30527574506540844 1107 3 0.34978503978412334 1107 4 0.31612679698857704 1107 5 0.167724356323383 1107 6 0.2175791993254208 1107 7 -0.05030357919494577 1107 8 0.1226318631916854 1107 9 0.3363402949576084 1107 10-11 0.4258195652936365 1107 12-13 0.24071516884458077 1107 14-15 0.6545949609643884 1107 16-17 0.6453028962064948 1107 18-19 0.31921058786712564 1107 20-21 0.4417902072550035 1107 22-23 0.3949857807869108 1107 24-25 0.46247073208989065 1107 26-27 0.4976321520795324 1107 28-29 0.4949387305980153 1107 30-31 0.5510896043953935 1107 32-33 0.6906074788027752 1107 34-35 0.8328919423872705 1107 36-37 0.7936944597362654 1107 38-39 1.0444326979884337 1107 40-41 0.8638805580905426 1107 42-43 0.92437734125361 1107 44-45 1.2952023165718316 1107 46-47 0.9655460927174317 1107 48-49 1.1051369971002742 1107 50-51 1.0315050584787961 1107 52-53 1.1869949388441512 1107 54-55 1.3679415549484801 1107 56-57 1.2007729592745058 1107 58-59 1.0729767874447909 1107 60-61 1.0876036408325298 1107 62-63 1.3659500178953614 1107 64-65 1.3157043457943125 1107 66-67 1.3056567604136227 1107 68-69 1.28923623017484 1107 70-71 1.2336231948097236 1107 72-73 1.3302568348182646 1107 74-75 1.5887311328079434 1107 76-77 1.361508293591644 1107 78-79 1.8369979547574538 1107 80-81 1.724417125644063 1107 82-83 1.8079701627371207 1107 84-85 1.5822405797419918 1107 86-87 1.437366769958988 1107 88-89 1.398728073782788 1107 90-91 1.1490631851545352 1107 92-93 1.3727281047419169 1107 94-95 1.2692884379362503 1107 96-97 1.4804086234333589 1107 98-99 1.2268715911179804 1107 100-101 1.2581692181871276 1108 1 0.3233532768144265 1108 2 0.21535546212598078 1108 3 0.2440757263613449 1108 4 0.2568021464524577 1108 5 0.39799859423951034 1108 6 0.4682388299322824 1108 7 0.4905303623084478 1108 8 0.4255566933718953 1108 9 0.8364806418952426 1108 10-11 0.5686892391273517 1108 12-13 0.6646927414039467 1108 14-15 0.8220955223521429 1108 16-17 0.8352098461868422 1108 18-19 0.6512609162789573 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-1.775437397464291 2316 74-75 -1.6592928684881727 2316 76-77 -1.5855852813747973 2316 78-79 -1.5989426553429986 2316 80-81 -1.7662786110591746 2316 82-83 -1.6090755022168217 2316 84-85 -1.31411930083015 2316 86-87 -1.5335487278885331 2316 88-89 -1.4240714216576578 2316 90-91 -1.032176110313685 2316 92-93 -1.3295759733953787 2316 94-95 -1.389031587060277 2316 96-97 -1.525311068276121 2316 98-99 -1.9208278024847552 2316 100-101 -1.5037157052031418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2781.0 3 502.0 4 434.0 5 423.0 6 424.0 7 583.0 8 767.0 9 907.0 10 1186.0 11 1718.0 12 2782.0 13 3929.0 14 4318.0 15 5432.0 16 6972.0 17 8786.0 18 7135.0 19 4282.0 20 4227.0 21 4143.0 22 4299.0 23 4601.0 24 5089.0 25 5509.0 26 5966.0 27 6570.0 28 7333.0 29 8081.0 30 9459.0 31 10687.0 32 12594.0 33 15242.0 34 20885.0 35 37188.0 36 38885.0 37 56156.0 38 48713.0 39 6846.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.51776407596373 21.139236688937444 15.120643284633712 15.222355950465108 2 14.046166949196637 26.1016614953184 39.66090926269262 20.191262292792338 3 20.966310842858785 33.7329733104759 31.091953708217172 14.208762138448142 4 10.23005223355273 19.733162417041232 39.8180179736011 30.218767375804934 5 9.249346650522405 44.309695870612146 32.54310462905639 13.897852849809064 6 21.497135101707993 41.02386594355576 19.331359554312673 18.14763940042358 7 21.61934345023878 42.38426907866829 20.564320803514004 15.432066667578923 8 23.58241637926315 32.0999616795314 21.209558219740515 23.108063721464937 9 27.27265261878562 20.65109684059542 22.669316383902423 29.40693415671654 10-11 21.69038408116515 30.03748167995654 25.979634817045888 22.292499421832428 12-13 28.055997602683025 29.968377856563272 26.529213195642686 15.446411345111018 14-15 24.451530350410213 29.336334959594367 31.206968517137323 15.005166172858091 16-17 15.011458554238297 33.782417447102524 32.28274923210584 18.923374766553337 18-19 14.084807742839626 32.96920162914952 37.84488044144697 15.101110186563895 20-21 16.710154530221555 30.8436189203693 35.94634701946139 16.49987952994776 22-23 15.751128366103979 30.673898431307588 35.182708379820966 18.392264822767466 24-25 15.957682796243706 31.545788408503974 37.02166524714704 15.474863548105283 26-27 17.34230657605809 29.776937524983982 38.004539555463076 14.876216343494855 28-29 14.807691254661243 30.638117871242944 39.43355984733577 15.120631026760048 30-31 14.451559385882302 33.70789439102915 37.67972907860443 14.160817144484112 32-33 14.18996157179175 33.820382694026854 36.17659178619295 15.81306394798844 34-35 15.331377478593266 31.26926194600689 36.34227732264608 17.057083252753763 36-37 16.587018437191876 31.32660992416939 37.1497194459779 14.936652192660826 38-39 17.519815286117083 30.292472799453108 37.6390227295814 14.54868918484841 40-41 14.71495749137337 31.599954979562366 39.0192681982766 14.665819330787667 42-43 13.773693185094471 33.604164893310305 36.835197302484715 15.786944619110512 44-45 14.656515799518086 33.86705476113655 34.830059930390135 16.64636950895523 46-47 14.976699098445966 32.8460328052528 32.99569681641306 19.181571279888175 48-49 15.966223843253147 32.24554590321969 33.920896010194916 17.867334243332245 50-51 17.405393974929183 32.77002486759482 34.18642045321912 15.638160704256876 52-53 15.144515973107175 33.14914092367184 35.54332183503977 16.163021268181218 54-55 13.821381547784947 32.13300620437505 36.15438583002566 17.89122641781434 56-57 15.847583234979275 34.30433947245629 32.706749375566105 17.141327916998325 58-59 15.240265995548445 32.17360518378448 34.01858565676913 18.567543163897938 60-61 15.170095180876586 31.203388598321336 34.8258583630645 18.80065785773758 62-63 18.33675026124737 30.693167196942756 33.769775498910384 17.200307042899496 64-65 17.268217126646228 30.964343631437803 35.30429504968713 16.463144192228842 66-67 15.115164465733558 31.190811012722197 35.29023203181648 18.403792489727756 68-69 14.629218606644287 33.25660226017877 34.619357107146975 17.49482202602996 70-71 15.437182672275393 32.7496631879926 32.92600023896142 18.88715390077058 72-73 15.492172480087888 30.909365558912388 32.790030211480364 20.808431749519364 74-75 16.0535043568422 31.848001483180322 33.44296965660256 18.65552450337492 76-77 17.658987592384356 29.607753489770616 34.13048522468123 18.602773693163797 78-79 16.104163520803876 29.619064614877754 35.313190659876184 18.963581204442185 80-81 16.11094791114723 31.938431212130364 33.70304988702597 18.247570989696438 82-83 16.078160566064156 31.040447667346697 32.94696031565161 19.934431450937538 84-85 16.50393333241807 28.96223177144112 32.91547110751109 21.61836378862972 86-87 17.870185786227765 29.19584163961975 33.308345827086455 19.62562674706603 88-89 19.225065701488685 27.351728834783756 33.83647263330468 19.58673283042288 90-91 17.376955973598992 27.84040189300615 35.09930591605622 19.68333621733864 92-93 17.38706469848211 29.83508122332156 33.703558224238996 19.074295853957338 94-95 17.674542528003165 29.553844632566623 32.53489751858179 20.23671532084842 96-97 17.72127734379292 27.81774283924981 31.761236911785712 22.69974290517156 98-99 18.921041934456127 27.35599793458796 32.089004976764116 21.633955154191796 100-101 20.5938201514413 26.65641497387878 33.07447809043915 19.675286784240768 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6100.0 1 3757.0 2 1783.5 3 2049.5 4 1629.0 5 1592.5 6 2738.5 7 5073.5 8 7238.0 9 7125.5 10 5060.0 11 3510.0 12 3159.0 13 2902.5 14 2597.5 15 2389.5 16 2198.5 17 2093.0 18 1988.0 19 1998.0 20 2153.5 21 2280.5 22 2454.5 23 2637.0 24 2773.5 25 3225.0 26 3930.0 27 4546.0 28 4908.0 29 5236.5 30 5862.5 31 6442.5 32 7624.0 33 9231.0 34 10687.5 35 11280.0 36 11771.0 37 12992.0 38 12402.5 39 10698.5 40 9862.0 41 9206.0 42 8246.0 43 8007.5 44 8129.5 45 7756.0 46 8575.0 47 11682.5 48 29505.0 49 30007.5 50 11502.5 51 6089.0 52 3298.0 53 2388.5 54 1873.5 55 1486.0 56 1187.0 57 951.5 58 911.5 59 889.5 60 816.0 61 790.5 62 765.5 63 636.0 64 532.5 65 515.5 66 457.5 67 412.0 68 382.0 69 335.5 70 288.5 71 245.5 72 220.0 73 180.5 74 148.5 75 130.5 76 101.5 77 69.5 78 49.5 79 34.5 80 26.0 81 20.5 82 14.5 83 11.0 84 7.5 85 5.5 86 4.0 87 3.0 88 2.5 89 1.0 90 0.0 91 0.5 92 1.5 93 1.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.295761750515943 2 0.18642284089821914 3 0.18396271543182036 4 0.20337037188896634 5 0.21676438831713749 6 0.10305192231470472 7 0.12027280057949623 8 0.13530690065193327 9 0.14022715158473084 10-11 0.12478303060122733 12-13 0.11658261237989805 14-15 0.13052332335615782 16-17 0.10592206869216997 18-19 0.13421351155575603 20-21 0.16373501715254143 22-23 0.16250495441934204 24-25 0.188062924542485 26-27 0.16428171170063008 28-29 0.16100154441209835 30-31 0.1538945152869463 32-33 0.16633181625596238 34-35 0.35220796260609294 36-37 0.47398417319283287 38-39 0.43626224937471814 40-41 0.42532835841294575 42-43 0.4504763076250222 44-45 0.4544398430986647 46-47 0.46086350403870596 48-49 0.4734374786447442 50-51 0.4664671231566143 52-53 0.47097735317834544 54-55 0.4527997594543988 56-57 0.4113876474366859 58-59 0.4012737982970465 60-61 0.3604083808274222 62-63 0.46920059589705737 64-65 0.4664671231566143 66-67 0.4775376877554089 68-69 0.49011166236144715 70-71 0.4809545286809627 72-73 0.47425752046687714 74-75 0.4790410977626526 76-77 0.454713190372709 78-79 0.43735563847089537 80-81 0.43516886027854085 82-83 0.44719614033649047 84-85 0.44828952943266775 86-87 0.4518430439952438 88-89 0.4614101985867946 90-91 0.48054450776989627 92-93 0.4857381059767381 94-95 0.4835513277843837 96-97 0.48300463323629506 98-99 0.47644429865923166 100-101 0.45580657946888625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 365835.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.457140811087676 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95997723324244 21.131009317112394 2 9.036997326621776 4.96260216212954 3 3.6740281528126584 3.0263492500703255 4 2.130999708106757 2.3404463621549585 5 1.367999599647121 1.8780678818511285 6 0.9299997249407905 1.5321080041183257 7 0.7599997751733119 1.460719459033002 8 0.5799998284210206 1.2740109567490123 9 0.5069998500162933 1.2528689645787932 >10 3.461998975850901 18.269701519638016 >50 0.350999896165126 6.588613559945804 >100 0.20399993965152624 10.227781926486964 >500 0.008999997337567333 1.7902050512938257 >1k 0.02099999378765711 11.454566615011629 >5k 0.004999998520870741 9.887038909821989 >10k+ 9.999997041741481E-4 2.9239100600042867 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10649 2.910875121297853 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8038 2.197165388768161 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7960 2.1758443013927042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7215 1.9722005822296935 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6567 1.795071548648981 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6229 1.7026801700220047 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4857 1.3276477100332118 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3287 0.8984924897836456 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2912 0.7959872620170295 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2850 0.7790397310262823 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2720 0.7435045854005221 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2520 0.6888351305916602 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2462 0.6729809886970902 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2027 0.5540749244878155 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1857 0.5076058879002829 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1724 0.4712507004523897 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1644 0.449382918528845 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1522 0.4160345510954392 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1477 0.4037339237634453 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1435 0.3922533382535843 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1396 0.3815927945658562 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1232 0.33676384162258943 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1232 0.33676384162258943 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1231 0.3364904943485451 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1176 0.32145639427610806 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1106 0.3023220850930064 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1051 0.28728798502056935 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 900 0.24601254663987865 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 874 0.23890551751472658 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 853 0.2331652247597961 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 785 0.21457761012478302 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 730 0.199543510052346 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 715 0.19544330094168136 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 610 0.16674183716702884 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 545 0.14897426435414873 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 508 0.13886041521450926 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 468 0.1279265242527369 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 449 0.122732926045895 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 446 0.12191288422376209 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 443 0.12109284240162915 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 440 0.12027280057949623 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 439 0.11999945330545192 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 435 0.11890606420927466 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 409 0.11179903508412262 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 404 0.11043229871390108 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 394 0.10769882597345798 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 387 0.10578539505514781 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 386 0.1055120477811035 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 383 0.10469200595897057 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 370 0.10113849139639455 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.733472740443096E-4 0.0 16-17 0.0 0.0 0.0 2.733472740443096E-4 0.0 18-19 0.0 0.0 0.0 8.200418221329289E-4 0.0 20-21 0.0 0.0 0.0 0.002733472740443096 0.0 22-23 0.0 0.0 0.0 0.006286987303019121 0.0 24-25 0.0 0.0 0.0 0.01339401642817117 0.0 26-27 0.0 0.0 0.0 0.028974811048696817 0.0 28-29 0.0 0.0 0.0 0.056582885727172086 0.0 30-31 0.0 0.0 0.0 0.12901991334891413 0.0 32-33 0.0 0.0 0.0 0.22715158473082128 0.0 34-35 0.0 0.0 0.0 0.3873330873207867 0.0 36-37 0.0 0.0 0.0 0.6061475801932565 0.0 38-39 0.0 0.0 0.0 0.8554402941216669 0.0 40-41 0.0 0.0 0.0 1.1296076099881094 0.0 42-43 0.0 0.0 0.0 1.3864173739527383 0.0 44-45 0.0 0.0 0.0 1.654571049790206 0.0 46-47 0.0 0.0 0.0 1.9940683641532384 0.0 48-49 0.0 0.0 1.366736370221548E-4 2.3770278950893164 0.0 50-51 0.0 0.0 2.733472740443096E-4 2.741536485027403 0.0 52-53 0.0 0.0 2.733472740443096E-4 3.0516489674306726 0.0 54-55 0.0 0.0 2.733472740443096E-4 3.3766588762693566 0.0 56-57 0.0 0.0 2.733472740443096E-4 3.741030792570421 0.0 58-59 0.0 0.0 2.733472740443096E-4 4.192190468380554 0.0 60-61 0.0 0.0 2.733472740443096E-4 4.822939303237798 0.0 62-63 0.0 0.0 2.733472740443096E-4 5.349542826684161 0.0 64-65 0.0 0.0 2.733472740443096E-4 5.7905886533546544 0.0 66-67 0.0 0.0 2.733472740443096E-4 6.301884729454535 0.0 68-69 0.0 0.0 2.733472740443096E-4 6.871267101288833 0.0 70-71 0.0 0.0 2.733472740443096E-4 7.523473697158555 0.0 72-73 0.0 0.0 2.733472740443096E-4 8.224746128719232 0.0 74-75 0.0 0.0 2.733472740443096E-4 8.946109584922166 0.0 76-77 0.0 0.0 2.733472740443096E-4 9.586835595282025 0.0 78-79 0.0 0.0 2.733472740443096E-4 10.310385829677314 0.0 80-81 0.0 0.0 2.733472740443096E-4 11.119220413574425 0.0 82-83 0.0 0.0 2.733472740443096E-4 11.90919403556248 0.0 84-85 0.0 0.0 2.733472740443096E-4 12.920442275889403 0.0 86-87 0.0 0.0 2.733472740443096E-4 13.998660598357183 0.0 88-89 0.0 1.366736370221548E-4 2.733472740443096E-4 14.840843549687701 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGGT 20 0.002093083 71.0765 4 TCTAGAC 20 0.002093083 71.0765 3 GACATAT 95 1.94741E-8 39.94089 1 CTTATGT 110 1.6516424E-9 38.768997 2 TATGTAC 150 2.5465852E-11 34.748512 4 GCTTATG 125 5.7116267E-9 34.14946 1 CATATAG 130 8.374627E-9 32.836018 1 CAATAGG 30 0.00399744 31.589554 26-27 GGTATGC 60 0.0040028887 31.576578 8 AGAGCAG 30 0.004008173 31.572254 10-11 ATGTATT 80 4.526727E-4 29.64363 1 GTACTAG 115 3.899706E-6 28.870314 1 CATGGGG 885 0.0 28.377056 4 CTAGTTC 120 5.471242E-6 27.64086 4 GTATGGT 70 0.008444888 27.102749 1 ATGGGGA 420 0.0 27.07676 5 TGAACCT 45 8.892727E-4 26.415081 36-37 CATGGGT 220 7.8216544E-11 25.845999 4 TCCTAGC 110 9.617355E-5 25.845999 2 CATGGGC 465 0.0 25.475447 4 >>END_MODULE