##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939355_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 365835 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09959407929804 33.0 31.0 34.0 30.0 34.0 2 32.25215465988765 33.0 31.0 34.0 30.0 34.0 3 32.284303032788 34.0 31.0 34.0 30.0 34.0 4 35.863996063799256 37.0 35.0 37.0 35.0 37.0 5 35.852777891672474 37.0 35.0 37.0 35.0 37.0 6 35.949007066027036 37.0 35.0 37.0 35.0 37.0 7 35.897934861344595 37.0 35.0 37.0 35.0 37.0 8 35.92197848756953 37.0 35.0 37.0 35.0 37.0 9 37.48046250358768 39.0 37.0 39.0 34.0 39.0 10-11 37.44208864652097 39.0 37.0 39.0 34.0 39.0 12-13 37.35769540913253 39.0 37.0 39.0 34.0 39.0 14-15 38.33403993603673 40.0 38.0 41.0 33.0 41.0 16-17 38.351750105922065 40.0 37.5 41.0 33.5 41.0 18-19 38.62502357620239 40.0 38.0 41.0 34.0 41.0 20-21 38.659366927713315 40.0 38.0 41.0 34.0 41.0 22-23 38.53321169379638 40.0 38.0 41.0 34.0 41.0 24-25 38.39661322727459 40.0 37.5 41.0 34.0 41.0 26-27 38.01554799294764 40.0 36.5 41.0 33.5 41.0 28-29 37.690518949799774 39.5 36.0 41.0 33.0 41.0 30-31 37.25879563191056 39.0 35.5 41.0 33.0 41.0 32-33 36.7848264928178 39.0 35.0 41.0 32.5 41.0 34-35 36.49408476498968 38.0 35.0 41.0 31.5 41.0 36-37 36.08563696748534 38.0 35.0 41.0 31.0 41.0 38-39 35.70877171402408 38.0 35.0 40.5 30.0 41.0 40-41 35.31682179124469 38.0 35.0 40.0 29.5 41.0 42-43 34.97132313748001 38.0 35.0 40.0 26.0 41.0 44-45 34.47317096505255 38.0 35.0 40.0 23.5 41.0 46-47 33.73249415720201 38.0 33.5 40.0 17.5 41.0 48-49 33.0995407765796 38.0 33.0 40.0 5.0 41.0 50-51 32.733629915125675 37.5 33.0 40.0 2.0 41.0 52-53 32.295487036505534 37.0 33.0 40.0 2.0 41.0 54-55 32.04222668689437 37.0 33.0 40.0 2.0 41.0 56-57 31.761455300887015 36.5 32.5 40.0 2.0 41.0 58-59 31.442657208850985 36.0 31.5 40.0 2.0 41.0 60-61 31.01092432380718 35.5 31.0 39.5 2.0 41.0 62-63 30.56137603017754 35.0 31.0 39.0 2.0 40.5 64-65 30.120573482580944 35.0 30.0 39.0 2.0 40.0 66-67 29.63799390435579 35.0 29.0 38.5 2.0 40.0 68-69 29.135136058605656 35.0 28.5 37.5 2.0 40.0 70-71 28.590467013817705 35.0 27.5 37.0 2.0 39.0 72-73 28.02171607418645 34.5 26.0 36.5 2.0 39.0 74-75 27.456962838438095 34.0 26.0 36.0 2.0 38.0 76-77 25.540423961622043 31.5 21.0 34.5 2.0 36.0 78-79 26.27852583815108 33.0 22.5 35.0 2.0 37.0 80-81 26.23953284950866 34.0 22.5 35.0 2.0 36.0 82-83 25.869233397569943 33.0 20.0 35.0 2.0 36.0 84-85 25.43353971052524 33.0 16.0 35.0 2.0 35.5 86-87 24.804230049065836 33.0 4.5 35.0 2.0 35.0 88-89 24.09821640903686 32.0 2.0 35.0 2.0 35.0 90-91 23.50844916424071 31.5 2.0 34.5 2.0 35.0 92-93 23.179787335820794 31.0 2.0 34.0 2.0 35.0 94-95 22.718050487241516 31.0 2.0 34.0 2.0 35.0 96-97 21.978479369114492 31.0 2.0 34.0 2.0 35.0 98-99 21.09240777946342 30.0 2.0 34.0 2.0 35.0 100-101 17.586719422690557 22.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.250909952831627 1101 2 0.10556091113416244 1101 3 0.48221030164960155 1101 4 0.21550815050562733 1101 5 0.08675971716940722 1101 6 0.11362713456615836 1101 7 0.15397600149891844 1101 8 0.21112358850258062 1101 9 0.5906502129971258 1101 10-11 0.40583898194679335 1101 12-13 0.1366387864753449 1101 14-15 0.6199649751437946 1101 16-17 0.15699526229626315 1101 18-19 0.44785371241399474 1101 20-21 0.3880180883761568 1101 22-23 0.21613737766897145 1101 24-25 0.24410429219805252 1101 26-27 0.2984216344480686 1101 28-29 0.2111892140526095 1101 30-31 0.3460026207378064 1101 32-33 0.7355341002997662 1101 34-35 0.8045968497778233 1101 36-37 0.19555912518730878 1101 38-39 0.13234820175973283 1101 40-41 0.10419508982073467 1101 42-43 0.08530846163741757 1101 44-45 0.40872289100176573 1101 46-47 0.3506074027934005 1101 48-49 0.6013753391916694 1101 50-51 0.9080902641403057 1101 52-53 0.6424625067288687 1101 54-55 0.4962705472433342 1101 56-57 0.6716461702451859 1101 58-59 0.8585051782901374 1101 60-61 0.7599163775891675 1101 62-63 0.567523262111461 1101 64-65 0.7287020877977746 1101 66-67 0.7137949197433642 1101 68-69 0.8178248880546413 1101 70-71 0.878194585661582 1101 72-73 1.0274021723178066 1101 74-75 1.152971505432042 1101 76-77 1.292644390349217 1101 78-79 0.9666137270517687 1101 80-81 1.304217167147911 1101 82-83 1.2663536944261082 1101 84-85 1.2495969527734268 1101 86-87 1.4383087131939547 1101 88-89 1.854848500325815 1101 90-91 1.7443886715352122 1101 92-93 1.6099153335469083 1101 94-95 1.571939242370707 1101 96-97 1.6551564735077768 1101 98-99 1.783121233286682 1101 100-101 1.3954500446376485 1102 1 0.13598538651387315 1102 2 0.1369826026177705 1102 3 0.17202306886174767 1102 4 -0.05507025629569284 1102 5 0.09812145484693247 1102 6 0.1409110305807033 1102 7 0.20579181554384007 1102 8 0.2366481843058139 1102 9 0.4242046874184737 1102 10-11 0.26400891658731496 1102 12-13 0.4159252378980227 1102 14-15 0.4921159407932052 1102 16-17 0.2655607937720106 1102 18-19 0.3234643075292283 1102 20-21 0.2864553580208593 1102 22-23 0.5638940241872348 1102 24-25 0.4132329963112724 1102 26-27 0.4197298068398396 1102 28-29 0.2858464453661824 1102 30-31 0.27041168260817017 1102 32-33 0.8432592956410687 1102 34-35 0.8084447047092738 1102 36-37 0.9204104692336941 1102 38-39 1.1358668021823561 1102 40-41 1.1482463945531478 1102 42-43 0.8958249489340915 1102 44-45 1.0323751589264774 1102 46-47 0.887675563390971 1102 48-49 0.9617124564947304 1102 50-51 0.6496303889621586 1102 52-53 0.8119892648005234 1102 54-55 0.5908501334637819 1102 56-57 0.9574783978730785 1102 58-59 1.0498364476399757 1102 60-61 0.7997070306925131 1102 62-63 0.7105327466070115 1102 64-65 0.7425307771682625 1102 66-67 0.7884914650281445 1102 68-69 0.7321744875435634 1102 70-71 0.27088710126431437 1102 72-73 0.2047180109339557 1102 74-75 0.584633994989268 1102 76-77 0.2534237898283074 1102 78-79 0.5574879714863847 1102 80-81 0.83922625850375 1102 82-83 0.8854209704593288 1102 84-85 0.7886406404239281 1102 86-87 1.215919406594118 1102 88-89 1.358558756358054 1102 90-91 1.4406784155029762 1102 92-93 1.3288597534201188 1102 94-95 1.060921501941202 1102 96-97 0.7929568568189964 1102 98-99 0.6542746069168359 1102 100-101 0.30125868388281773 1103 1 -0.09581998360925326 1103 2 -0.0012164776373850827 1103 3 0.23788866743763037 1103 4 0.24102824541029122 1103 5 0.16431341449683856 1103 6 0.0642458473734635 1103 7 0.08880620510028336 1103 8 0.14962845429542426 1103 9 0.18610944932323292 1103 10-11 0.15570154379396683 1103 12-13 0.18646373367015912 1103 14-15 0.3191297369658841 1103 16-17 0.1758411676037852 1103 18-19 0.2319793646424202 1103 20-21 0.0971362658980297 1103 22-23 0.16632689764814046 1103 24-25 0.354131972724268 1103 26-27 0.43760549293982365 1103 28-29 0.4304392357533686 1103 30-31 0.26904689993497755 1103 32-33 0.05207104463972456 1103 34-35 0.359668558736864 1103 36-37 0.6693349611488415 1103 38-39 0.8995665499942547 1103 40-41 0.7847840002496369 1103 42-43 0.6626547961377725 1103 44-45 0.15167496607676156 1103 46-47 0.10074484094622704 1103 48-49 -0.13226469823009523 1103 50-51 -0.42556616941196523 1103 52-53 -0.670698763681898 1103 54-55 -0.45794084710289695 1103 56-57 -0.3999759857495313 1103 58-59 -0.30561526575565523 1103 60-61 -0.16451192791896574 1103 62-63 -0.4234829780629177 1103 64-65 -0.41665925398438475 1103 66-67 -0.5217740742093575 1103 68-69 -0.6132342055286806 1103 70-71 -0.9627725931430433 1103 72-73 -0.8317454217911582 1103 74-75 -0.794478896273155 1103 76-77 -0.7983637787816917 1103 78-79 -0.8148306755986177 1103 80-81 -0.3818982037422103 1103 82-83 -0.1292801124660521 1103 84-85 -0.008347936938481126 1103 86-87 -0.27279146716348635 1103 88-89 0.01580545566082847 1103 90-91 0.3700360775709193 1103 92-93 0.05031057674010242 1103 94-95 -0.039019159681700444 1103 96-97 0.5678567233076457 1103 98-99 1.0416206980012461 1103 100-101 1.0378407933621219 1104 1 0.28796951349799826 1104 2 0.03202228515745276 1104 3 0.3119040212427038 1104 4 0.2385805373551051 1104 5 0.04117701040249244 1104 6 -0.10055722338754691 1104 7 0.1940854663374907 1104 8 0.13466405732168596 1104 9 0.2664665921803788 1104 10-11 0.17045732928572477 1104 12-13 0.18140142837420825 1104 14-15 0.22991355984083128 1104 16-17 0.5369893652005828 1104 18-19 -0.057587279772356226 1104 20-21 0.055205358020856465 1104 22-23 0.23750513529834194 1104 24-25 -0.08944557511729556 1104 26-27 -0.05546860585857161 1104 28-29 0.16888215965190057 1104 30-31 -0.08534228564580104 1104 32-33 -0.09543118054941147 1104 34-35 0.11886137137594233 1104 36-37 0.1813231676463971 1104 38-39 -0.1058990708335088 1104 40-41 -0.06931312925637201 1104 42-43 0.1340193933785372 1104 44-45 0.13068865098997406 1104 46-47 0.5100961983116079 1104 48-49 0.5842322977645651 1104 50-51 0.4465549921367611 1104 52-53 0.3349059314671834 1104 54-55 0.6576160064796639 1104 56-57 0.5033117312064128 1104 58-59 0.6730507333542661 1104 60-61 0.9699451259306109 1104 62-63 0.9142033815276456 1104 64-65 0.5145545866920749 1104 66-67 0.7101184491551287 1104 68-69 0.787035598654672 1104 70-71 0.9590815457087558 1104 72-73 0.9771386458545912 1104 74-75 1.044653836259112 1104 76-77 1.2134436310981478 1104 78-79 0.9122498762482927 1104 80-81 0.7293056235831159 1104 82-83 0.585520176808533 1104 84-85 0.8104660372493235 1104 86-87 0.6517328986576132 1104 88-89 1.1507214547707498 1104 90-91 0.9425633361378978 1104 92-93 1.051478801039167 1104 94-95 0.9090365813062817 1104 96-97 0.9643854282475672 1104 98-99 1.1436595275517547 1104 100-101 1.4732824934066286 1105 1 0.08473822098099504 1105 2 0.30309857281537234 1105 3 0.2206139337791484 1105 4 0.2565591573745394 1105 5 0.1802076226473872 1105 6 0.1880117756406321 1105 7 0.16620645759113017 1105 8 0.10150079201556395 1105 9 0.163757651461232 1105 10-11 0.3633934322983663 1105 12-13 0.17763563634311197 1105 14-15 0.2516883411819393 1105 16-17 0.2969504925086497 1105 18-19 0.19991393499926602 1105 20-21 0.06507537745720526 1105 22-23 0.01611300701846119 1105 24-25 0.1910755619089457 1105 26-27 0.520636836318296 1105 28-29 0.46689600804840126 1105 30-31 0.5443345851024972 1105 32-33 0.42187931507741183 1105 34-35 0.3956167163710802 1105 36-37 0.49200101021199316 1105 38-39 0.4070686162413253 1105 40-41 0.5774009135035882 1105 42-43 0.4532917451764007 1105 44-45 0.3624367073553785 1105 46-47 0.4134611157071433 1105 48-49 0.26313778853230474 1105 50-51 0.6210265664807864 1105 52-53 0.4536802268996425 1105 54-55 0.25579992290337117 1105 56-57 0.266127571828374 1105 58-59 0.19036122898108943 1105 60-61 0.3733707819072869 1105 62-63 0.2821880754051982 1105 64-65 0.1911216420856583 1105 66-67 0.2828225307877794 1105 68-69 0.3563079504525355 1105 70-71 0.2338932561072049 1105 72-73 0.10863767403729341 1105 74-75 0.121822203606051 1105 76-77 0.18079664373177096 1105 78-79 0.2812974952959131 1105 80-81 0.1013901716880703 1105 82-83 0.08263509420406479 1105 84-85 0.09946020634553321 1105 86-87 0.368721479804357 1105 88-89 0.3179043993771664 1105 90-91 0.6924114896850284 1105 92-93 0.6079656815056396 1105 94-95 0.6730368242606062 1105 96-97 0.3103523864594244 1105 98-99 0.6117414031591437 1105 100-101 1.204271317507697 1106 1 0.06966940275602695 1106 2 0.03984343687395153 1106 3 -0.1006790352357001 1106 4 0.04395152295865046 1106 5 -0.11642265737159363 1106 6 -0.001373945425200418 1106 7 0.06430971882917902 1106 8 -0.1776283014823008 1106 9 0.01653580591238324 1106 10-11 0.01801408454597464 1106 12-13 0.03653708728894145 1106 14-15 0.18454395851191663 1106 16-17 -0.011667888398527282 1106 18-19 0.017198157658434354 1106 20-21 0.08327152634865342 1106 22-23 0.5130450024079778 1106 24-25 0.31576621890264533 1106 26-27 0.29952862559214566 1106 28-29 0.4677816613130261 1106 30-31 0.5026283677318304 1106 32-33 0.5955987197828208 1106 34-35 0.5150061665032979 1106 36-37 0.2969793138257941 1106 38-39 0.4908963333746854 1106 40-41 0.7010703148189279 1106 42-43 0.19157061154021449 1106 44-45 0.563170655419647 1106 46-47 0.4288392769384046 1106 48-49 0.36077576398826494 1106 50-51 0.523901337651715 1106 52-53 0.6621138495181285 1106 54-55 0.33905980492927057 1106 56-57 0.22078127716433116 1106 58-59 0.35972016857021316 1106 60-61 0.12339196978443212 1106 62-63 0.2810015542851225 1106 64-65 0.5882672112767899 1106 66-67 0.429595193341175 1106 68-69 0.4882745244575517 1106 70-71 0.5278453884551588 1106 72-73 0.3217538175046464 1106 74-75 0.18242376981392994 1106 76-77 0.005560187022844332 1106 78-79 0.31576593383411833 1106 80-81 0.04637372989540722 1106 82-83 0.45744016573433655 1106 84-85 0.423007565488529 1106 86-87 0.4230853349809749 1106 88-89 0.6942222853355347 1106 90-91 0.7553055841999097 1106 92-93 0.6067113591787034 1106 94-95 0.9040185857359617 1106 96-97 0.6871152178378246 1106 98-99 0.631166448924958 1106 100-101 0.6827232464520314 1107 1 0.06729166452199564 1107 2 0.020376663848246324 1107 3 0.007178239109265405 1107 4 -0.06780217404079991 1107 5 -0.26166411285419855 1107 6 0.13167201395382477 1107 7 0.14802911503707605 1107 8 -0.024448268347370572 1107 9 0.12630294096791772 1107 10-11 0.017277752705759042 1107 12-13 -0.19008054172253708 1107 14-15 -0.12038562975585165 1107 16-17 0.00953616756268616 1107 18-19 0.12141212341899887 1107 20-21 0.014524210891821099 1107 22-23 0.0834107138886111 1107 24-25 0.11896235929658161 1107 26-27 0.08738068425324741 1107 28-29 -0.1083864984709777 1107 30-31 0.05359633729678137 1107 32-33 -0.03896208895498887 1107 34-35 0.08168254865952207 1107 36-37 -0.1284440769823263 1107 38-39 -0.11767426244049517 1107 40-41 -0.24707965143252153 1107 42-43 -0.3729734763287098 1107 44-45 -0.4175389843913564 1107 46-47 -0.6262667241660935 1107 48-49 -0.23646989472192814 1107 50-51 -0.04466956496839458 1107 52-53 0.06249812269724231 1107 54-55 0.12411226857288682 1107 56-57 -0.04539884801208416 1107 58-59 0.03954621017296489 1107 60-61 -0.18926143268228302 1107 62-63 -0.07584326377955009 1107 64-65 -0.04568382265583537 1107 66-67 0.04306699796779512 1107 68-69 0.2528094081784822 1107 70-71 0.36530013472748024 1107 72-73 0.22138258353520612 1107 74-75 0.3328770741030631 1107 76-77 0.6712233458864354 1107 78-79 0.8851421994044664 1107 80-81 0.9079241686264616 1107 82-83 0.5657116267394322 1107 84-85 0.5923638887382054 1107 86-87 0.8838718294278074 1107 88-89 0.6459273537530343 1107 90-91 0.7870829051792327 1107 92-93 0.8638138933874515 1107 94-95 1.062161188669016 1107 96-97 0.9088508133461985 1107 98-99 -0.046412246540466384 1107 100-101 0.10439978201072364 1108 1 0.03936262393366263 1108 2 0.057161596201019904 1108 3 0.07615174160744687 1108 4 0.10889462043514442 1108 5 0.0755615088827355 1108 6 -0.0032294624959590124 1108 7 0.1084091745441711 1108 8 0.09173092662259563 1108 9 0.314655548614013 1108 10-11 -2.936838854949997E-4 1108 12-13 0.12643688936711328 1108 14-15 0.07290368142137282 1108 16-17 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-2.2713705998311156 2316 76-77 -2.1871672711574917 2316 78-79 -2.2793228054559727 2316 80-81 -2.3626429979707737 2316 82-83 -2.5655606502591333 2316 84-85 -2.5245903537281187 2316 86-87 -2.435594795577977 2316 88-89 -2.520409497610199 2316 90-91 -2.5645638568445612 2316 92-93 -2.4211089182590797 2316 94-95 -2.3286670527789326 2316 96-97 -2.1654704614767404 2316 98-99 -2.1928849586637824 2316 100-101 -1.5240545993252006 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 9.0 5 41.0 6 132.0 7 248.0 8 397.0 9 657.0 10 1225.0 11 1720.0 12 2489.0 13 3201.0 14 3935.0 15 5299.0 16 6822.0 17 9297.0 18 8511.0 19 4928.0 20 4391.0 21 4365.0 22 4602.0 23 4942.0 24 5582.0 25 5868.0 26 6011.0 27 6681.0 28 7615.0 29 8848.0 30 9914.0 31 11557.0 32 13604.0 33 16824.0 34 23354.0 35 37753.0 36 41344.0 37 60426.0 38 40817.0 39 2423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.141881869751295 21.825280410601728 15.690930077618424 15.341907642028552 2 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15.34680935394372 20-21 16.500334850410706 30.51703636885481 36.41450380636079 16.568124974373692 22-23 15.727582106687441 30.590430111935706 35.56207033225361 18.11991744912324 24-25 15.864802438257685 31.48099553077207 37.46907758962374 15.18512444134651 26-27 17.23522899667883 29.69562781035166 38.572170514029544 14.49697267893996 28-29 14.65619746607077 30.51498626429948 40.012573974606035 14.816242295023713 30-31 14.497109352576981 33.41260404280618 38.17636366121339 13.913922943403447 32-33 14.153648502740307 33.532193475200565 36.74484398704334 15.569314035015786 34-35 15.163306503968368 30.882881553068977 36.99623186147683 16.957580081485823 36-37 16.394961831017678 31.074677255113688 37.749362711262066 14.780998202606563 38-39 17.337085546916107 29.733468004816466 38.32943578753432 14.600010660733103 40-41 14.582658482347266 31.10328397866172 39.6895753803426 14.62448215864841 42-43 13.793258973661587 32.98978089819303 37.68562013697451 15.53133999117087 44-45 14.476473458409131 33.49738882518318 35.48204569590098 16.54409202050671 46-47 15.009109310357552 32.47206045356575 33.344312797180145 19.174517438896547 48-49 16.043478736257427 31.686502621407524 34.23724968154956 18.032768960785493 50-51 17.332540626238604 32.28203971735892 34.53141443546954 15.854005220932935 52-53 15.10011343911873 32.92549920045922 35.880246559241186 16.09414080118086 54-55 13.745267675318107 31.957986523979386 36.16083753604767 18.13590826465483 56-57 15.870766169263023 34.19538423269634 32.45087239259444 17.4829772054462 58-59 15.136464217522692 32.295187499060354 33.93366017136445 18.634688112052505 60-61 15.168239103307926 31.589643922713474 34.22882267479605 19.01329429918255 62-63 18.086766840426314 31.133774885124556 33.374837014905786 17.40462125954334 64-65 16.980469337269536 31.438626703295203 34.86640151981084 16.71450243962442 66-67 14.713367264934368 31.638530043681012 34.86868361059934 18.779419080785274 68-69 14.315216589080752 33.45889222072487 34.17944351353494 18.04644767665944 70-71 15.063211557122747 33.19542963357798 32.3059576038378 19.435401205461478 72-73 15.098610029111484 31.318490576352726 31.99106154413875 21.591837850397035 74-75 15.578219112740872 32.29916806643441 32.463723350312776 19.65888947051194 76-77 16.786947960927193 30.139747361324826 33.69200772248386 19.381296955264126 78-79 15.182254294969045 30.15129771618352 34.6668580097585 19.999589979088935 80-81 14.975603755791544 32.65679883007367 33.055885850178356 19.31171156395643 82-83 14.713737067257096 32.19292850602047 32.12349829841322 20.96983612830921 84-85 15.18840460863504 30.186286167261194 31.859991526234506 22.765317697869257 86-87 16.2103442803277 30.530868116172037 32.287157484580995 20.971630118919276 88-89 17.304196931349754 28.786600547336366 33.009321179448136 20.899881341865743 90-91 15.236621721809653 29.51313468793725 34.07385683882205 21.17638675143105 92-93 14.624079161370563 31.766916779422417 32.865636147443524 20.7433679117635 94-95 14.76854319570298 31.71443410280591 31.53949184741755 21.977530854073557 96-97 14.885399155356923 30.248335998469255 30.5163530006697 24.34991184550412 98-99 15.822980305328905 29.738406658739596 30.78546339196632 23.653149643965175 100-101 16.43352390220168 29.644415712569483 32.06559250152733 21.856467883701512 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5628.0 1 3537.5 2 1712.0 3 1926.5 4 1597.5 5 1582.5 6 2760.0 7 5061.0 8 6978.0 9 6880.5 10 5034.0 11 3511.0 12 3065.0 13 2837.5 14 2630.5 15 2334.5 16 2127.0 17 2061.5 18 2008.5 19 2070.5 20 2206.5 21 2316.0 22 2438.0 23 2692.5 24 2947.0 25 3349.0 26 4097.0 27 4852.5 28 5154.5 29 5387.5 30 6066.5 31 6733.5 32 7887.5 33 9442.0 34 10602.5 35 10999.0 36 11543.0 37 12791.0 38 12268.0 39 10723.0 40 9998.5 41 9396.5 42 8438.5 43 8157.0 44 8503.5 45 8164.0 46 8824.0 47 12081.5 48 29849.0 49 30540.0 50 12769.0 51 6852.0 52 2848.5 53 1475.0 54 1097.0 55 984.0 56 881.0 57 779.5 58 764.5 59 743.5 60 670.5 61 641.5 62 633.5 63 532.5 64 415.5 65 377.5 66 345.0 67 311.5 68 302.0 69 277.5 70 218.5 71 178.5 72 163.5 73 146.0 74 116.5 75 98.0 76 77.0 77 48.5 78 33.0 79 20.0 80 16.0 81 13.0 82 9.5 83 5.5 84 3.0 85 2.5 86 2.0 87 1.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30122869599682917 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.200418221329289E-4 16-17 6.83368185110774E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0012300627331993932 36-37 0.007517050036218514 38-39 0.0017767572812880123 40-41 0.0036901881995981796 42-43 1.366736370221548E-4 44-45 9.567154591550835E-4 46-47 1.366736370221548E-4 48-49 2.733472740443096E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.466945480886192E-4 58-59 0.0017767572812880123 60-61 4.1002091106646444E-4 62-63 0.0010933890961772384 64-65 0.0 66-67 2.733472740443096E-4 68-69 0.0010933890961772384 70-71 0.0 72-73 0.0 74-75 1.366736370221548E-4 76-77 0.2532562494020528 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.10305192231470472 88-89 0.3669687154044856 90-91 0.26801700220044555 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 1.366736370221548E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 365835.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.815463255292688 #Duplication Level Percentage of deduplicated Percentage of total 1 75.42830520319349 19.47216641382044 2 9.730840092331801 5.0241228969344105 3 3.840452341119417 2.9742916888761326 4 2.201351093793016 2.2731559309524787 5 1.4802736070815952 1.9106974455697239 6 1.0620274877702718 1.6450038951986552 7 0.7909616484191355 1.4293328959776948 8 0.5918976726456449 1.2224090095261524 9 0.5198958090679994 1.2079216040018042 >10 3.730331843883018 18.66551860811568 >50 0.3557739141483662 6.359971025188951 >100 0.22765295101755573 10.895075648858093 >500 0.011647360284619131 2.2745226673227004 >1k 0.022235869634272886 11.515027266390586 >5k 0.005294254674826878 10.23084177293042 >10k+ 0.0010588509349653756 2.8999412303360805 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10609 2.8999412303360805 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8264 2.2589418727021746 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7845 2.144409364877609 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7825 2.1389424193967224 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6813 1.8623149780638812 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6681 1.8262331378900325 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4546 1.2426367078054315 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3515 0.9608156682657483 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2895 0.7913403583582763 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2851 0.7793130783003266 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2710 0.740771112660079 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2698 0.7374909453715472 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2558 0.6992223270053439 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1864 0.5095193188185931 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1824 0.4985854278568207 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1804 0.49311848237593453 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1709 0.46715049134172504 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1680 0.4592234203944401 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1535 0.4195880656580152 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1420 0.3881531291429196 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1375 0.37585250181092567 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1335 0.3649186108491533 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1286 0.3515245944209821 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1248 0.34113739800729836 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1170 0.31981631063184224 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1096 0.2995886123525633 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 1007 0.27526070496261973 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 973 0.2659668976451132 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 965 0.26378011945275875 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 885 0.24191233752921398 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 869 0.23753878114450505 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 801 0.218951166509492 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 801 0.218951166509492 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 726 0.19845012095616876 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 628 0.17166208809982642 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 589 0.16100154441209835 RNA PCR Primer, Index 33 (95% over 22bp) AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 553 0.1511610425465032 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 531 0.1451474025175284 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 500 0.1366736370221548 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 464 0.12683313515655964 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 453 0.12382631514207224 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 453 0.12382631514207224 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 442 0.12081949512758483 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 434 0.11863271693523036 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 431 0.11781267511309743 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 428 0.11699263329096452 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 421 0.11507920237265434 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 416 0.11371246600243279 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 411 0.11234572963221125 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 408 0.11152568781007831 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 405 0.11070564598794538 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 403 0.11015895143985677 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 402 0.10988560416581244 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 402 0.10988560416581244 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 376 0.1027785750406604 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 370 0.10113849139639455 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 366 0.10004510230021732 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.733472740443096E-4 0.0 16-17 0.0 0.0 0.0 2.733472740443096E-4 0.0 18-19 0.0 0.0 0.0 8.200418221329289E-4 0.0 20-21 0.0 0.0 0.0 0.0028701463774652505 0.0 22-23 0.0 0.0 0.0 0.006833681851107739 0.0 24-25 0.0 0.0 0.0 0.014350731887326253 0.0 26-27 0.0 0.0 0.0 0.030478221055940516 0.0 28-29 0.0 0.0 0.0 0.058769663919526566 0.0 30-31 0.0 0.0 0.0 0.13148003881531292 0.0 32-33 0.0 0.0 0.0 0.23002173110828655 0.0 34-35 0.0 0.0 0.0 0.3918433173425178 0.0 36-37 0.0 0.0 0.0 0.6148946929626744 0.0 38-39 0.0 0.0 0.0 0.8666475323574836 0.0 40-41 0.0 0.0 0.0 1.1454617518826793 0.0 42-43 0.0 0.0 0.0 1.4067817458690395 0.0 44-45 0.0 0.0 0.0 1.6805390408244154 0.0 46-47 0.0 0.0 0.0 2.0259133215794005 0.0 48-49 0.0 0.0 0.0 2.4094195470635666 0.0 50-51 0.0 0.0 0.0 2.780078450667651 0.0 52-53 0.0 0.0 0.0 3.094291142181585 0.0 54-55 0.0 0.0 0.0 3.4238112810419996 0.0 56-57 0.0 0.0 0.0 3.7961102682903496 0.0 58-59 0.0 0.0 0.0 4.25096013230008 0.0 60-61 0.0 0.0 0.0 4.887449259912255 0.0 62-63 0.0 0.0 0.0 5.416512908825016 0.0 64-65 0.0 0.0 0.0 5.8650757855317295 0.0 66-67 0.0 0.0 0.0 6.382248828023563 0.0 68-69 0.0 0.0 0.0 6.949854442576571 0.0 70-71 0.0 0.0 0.0 7.60055762843905 0.0 72-73 0.0 0.0 0.0 8.302923449095903 0.0 74-75 0.0 0.0 0.0 9.029070482594612 0.0 76-77 0.0 0.0 0.0 9.674033375702162 0.0 78-79 0.0 0.0 0.0 10.40400727103749 0.0 80-81 0.0 0.0 0.0 11.218855494963577 0.0 82-83 0.0 0.0 0.0 12.012655978788253 0.0 84-85 0.0 0.0 0.0 13.02677436549264 0.0 86-87 0.0 0.0 0.0 14.111006327989394 0.0 88-89 0.0 0.0 0.0 14.95428266841609 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAACGC 20 0.0020805788 71.18398 8 GTAAGCG 20 5.411563E-4 47.455986 94-95 GCTTATG 75 1.3358476E-7 44.43194 1 CTTATGT 75 1.3693352E-7 44.292255 2 ATGGGCG 50 0.0016217445 37.96479 7 TAGGCAT 90 5.725233E-7 36.910213 5 CATATAG 105 4.6808054E-8 36.156944 3 GGATATG 200 0.0 35.704235 1 TGGGATA 175 0.0 35.25302 6 TGGGGAG 125 5.680704E-9 34.168312 6 ATATGAG 210 0.0 33.897133 3 TGGGACC 140 4.5838533E-10 33.897133 6 CATTCCG 100 1.3040117E-6 33.223732 9 GATTGGC 200 0.0 33.223732 9 TTAGGCA 100 1.3054068E-6 33.219193 4 GGCATTC 100 1.3054068E-6 33.219193 7 ATGGGGA 435 0.0 31.637323 5 GGGACCA 75 3.0911376E-4 31.637323 7 TAGGACA 220 0.0 30.199266 4 CATGGGG 840 0.0 29.377516 4 >>END_MODULE