##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939354_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 370350 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775293641150263 33.0 31.0 34.0 30.0 34.0 2 31.73102740650736 33.0 31.0 34.0 30.0 34.0 3 31.71220467125692 33.0 31.0 34.0 30.0 34.0 4 35.25303631699744 37.0 35.0 37.0 33.0 37.0 5 35.34691778047792 37.0 35.0 37.0 33.0 37.0 6 35.51122991764547 37.0 35.0 37.0 33.0 37.0 7 35.481900904549754 37.0 35.0 37.0 33.0 37.0 8 35.48586202241123 37.0 35.0 37.0 33.0 37.0 9 37.00500877548265 39.0 37.0 39.0 33.0 39.0 10-11 36.958321857702174 39.0 37.0 39.0 33.0 39.0 12-13 36.855474551100315 39.0 37.0 39.0 33.0 39.0 14-15 37.81464830565681 40.0 38.0 41.0 32.5 41.0 16-17 37.81220062103416 40.0 37.5 41.0 32.5 41.0 18-19 37.96572971513433 40.0 38.0 41.0 33.0 41.0 20-21 37.96194815714864 40.0 38.0 41.0 33.0 41.0 22-23 37.84051302821655 40.0 37.0 41.0 33.0 41.0 24-25 37.75592412582692 40.0 37.0 41.0 33.0 41.0 26-27 37.50275145132982 40.0 36.5 41.0 33.0 41.0 28-29 37.24801269069799 39.5 36.0 41.0 32.5 41.0 30-31 36.843055218036994 39.0 35.0 41.0 32.0 41.0 32-33 36.37059943296882 38.0 35.0 41.0 31.5 41.0 34-35 35.950673687052785 38.0 35.0 41.0 30.5 41.0 36-37 35.52092885108681 38.0 35.0 40.0 30.0 41.0 38-39 34.99958957742676 37.5 35.0 40.0 28.0 41.0 40-41 34.699127852031864 38.0 35.0 40.0 26.0 41.0 42-43 34.380363169974345 38.0 35.0 40.0 24.0 41.0 44-45 33.881977858782236 38.0 34.5 40.0 19.5 41.0 46-47 33.24703928716079 38.0 33.5 40.0 5.5 41.0 48-49 32.78572971513433 38.0 33.0 40.0 2.0 41.0 50-51 31.552477386256243 36.5 31.5 39.0 2.0 40.5 52-53 31.579088699878493 36.5 32.0 39.5 2.0 40.5 54-55 31.98525043877413 37.0 33.0 40.0 2.0 41.0 56-57 31.897968138247602 37.0 33.0 40.0 2.0 41.0 58-59 31.697356554610504 37.0 33.0 40.0 2.0 41.0 60-61 31.339003645200485 36.0 32.0 40.0 2.0 41.0 62-63 30.885644660456325 36.0 31.0 39.5 2.0 41.0 64-65 30.35889563926016 35.0 31.0 39.0 2.0 41.0 66-67 29.910352369380316 35.0 30.0 39.0 2.0 40.5 68-69 29.457739975698665 35.0 29.0 38.5 2.0 40.0 70-71 28.935724314837316 35.0 28.5 37.5 2.0 39.5 72-73 28.36688942891859 35.0 27.0 37.0 2.0 39.0 74-75 27.84846901579587 35.0 26.0 36.5 2.0 39.0 76-77 27.27333603348184 34.5 25.0 36.0 2.0 38.0 78-79 26.756798973943567 34.0 22.5 35.0 2.0 37.0 80-81 26.255386796273797 34.0 20.0 35.0 2.0 37.0 82-83 25.761486431753745 34.0 15.0 35.0 2.0 36.0 84-85 25.289962197920886 34.0 4.5 35.0 2.0 36.0 86-87 24.892100715539357 33.0 2.0 35.0 2.0 35.5 88-89 24.317626569461318 33.0 2.0 35.0 2.0 35.0 90-91 23.75624544349939 33.0 2.0 35.0 2.0 35.0 92-93 23.320822195220735 32.5 2.0 35.0 2.0 35.0 94-95 22.80146888078844 32.0 2.0 35.0 2.0 35.0 96-97 22.13840286215742 31.5 2.0 35.0 2.0 35.0 98-99 21.446140137707573 31.0 2.0 35.0 2.0 35.0 100-101 19.51867152693398 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44839561751896895 1101 2 0.19007651345020093 1101 3 0.4443791737779996 1101 4 0.5339691787252292 1101 5 0.43109467763139264 1101 6 0.39049093257253986 1101 7 0.4426264500377428 1101 8 0.2300001712241766 1101 9 0.6399053439617646 1101 10-11 0.5099295395576604 1101 12-13 0.47669889524423326 1101 14-15 0.6714212084292441 1101 16-17 0.725068487377456 1101 18-19 0.5895787415803042 1101 20-21 0.5037488145997528 1101 22-23 0.7269225667237649 1101 24-25 0.8602050766828881 1101 26-27 0.930481011380472 1101 28-29 1.0348181038765034 1101 30-31 1.1629467473118922 1101 32-33 1.4139875835125864 1101 34-35 1.315158847541305 1101 36-37 1.471750345100432 1101 38-39 1.536365145367867 1101 40-41 1.6354718207197223 1101 42-43 1.7583335565289957 1101 44-45 1.6995165489379431 1101 46-47 1.8000714175385824 1101 48-49 1.7293126481532184 1101 50-51 1.2653353253145596 1101 52-53 1.2385520497207452 1101 54-55 1.0159927736982084 1101 56-57 1.1728254964556015 1101 58-59 1.0153750589562982 1101 60-61 0.987978955385433 1101 62-63 1.3067219349104242 1101 64-65 1.3500855171041692 1101 66-67 1.303258440039631 1101 68-69 0.7456821419165536 1101 70-71 0.7050826547016911 1101 72-73 0.6793183059084917 1101 74-75 0.30872795694014954 1101 76-77 0.10644564243125743 1101 78-79 0.06414323893426044 1101 80-81 0.30513760766016773 1101 82-83 0.249751329866303 1101 84-85 0.16910886943099257 1101 86-87 0.4713583647576769 1101 88-89 0.5526058011706958 1101 90-91 0.5270419743122936 1101 92-93 0.3617447891695065 1101 94-95 0.4598152690163211 1101 96-97 0.27151287245224864 1101 98-99 0.5121986193282844 1101 100-101 0.1885396048792387 1102 1 0.036236703610157406 1102 2 -0.008767783994176881 1102 3 0.255601603253222 1102 4 0.3409033940589623 1102 5 0.163465886984703 1102 6 0.12015075989019408 1102 7 -0.09477523832639179 1102 8 0.0055900410393761035 1102 9 -0.03798823910075555 1102 10-11 0.08925669376030498 1102 12-13 0.21704560601092027 1102 14-15 0.21499081018298938 1102 16-17 0.43655147874333977 1102 18-19 0.2886730246024314 1102 20-21 0.3967069131709877 1102 22-23 0.29719735856269125 1102 24-25 0.5315798050639415 1102 26-27 0.5328933722058125 1102 28-29 0.5420532633865278 1102 30-31 0.2532396150310632 1102 32-33 0.22724354951454728 1102 34-35 0.5150127021266115 1102 36-37 0.5294152875075966 1102 38-39 0.8269829943381168 1102 40-41 0.7965827181679472 1102 42-43 0.8536020987284871 1102 44-45 0.7512924080229979 1102 46-47 0.6499598855115778 1102 48-49 0.5154115270640531 1102 50-51 0.6782393885416838 1102 52-53 0.1577201746366157 1102 54-55 0.16702252198195566 1102 56-57 0.603637180396909 1102 58-59 0.39226196932685653 1102 60-61 0.6230528934274737 1102 62-63 0.6214441624742157 1102 64-65 0.2564803404797438 1102 66-67 0.2794376989285432 1102 68-69 0.17219118947150136 1102 70-71 0.016511803020058835 1102 72-73 0.2141191827809834 1102 74-75 0.18858104233907014 1102 76-77 0.05067001410212768 1102 78-79 0.07796455693514659 1102 80-81 0.058853739421795837 1102 82-83 -0.2870446242132694 1102 84-85 -0.22034943105167315 1102 86-87 -0.14669732433718252 1102 88-89 -0.26406823686557246 1102 90-91 0.09514381459073817 1102 92-93 0.2169358358398874 1102 94-95 -0.0046723566975828135 1102 96-97 -0.07769294259688664 1102 98-99 -0.05419658864048671 1102 100-101 0.27658260239861576 1103 1 0.23651422442421932 1103 2 0.39119257017524234 1103 3 0.5316687368596682 1103 4 0.38504241877152623 1103 5 0.4799056913986064 1103 6 0.45826361835457874 1103 7 0.46165399386602246 1103 8 0.5447733369294312 1103 9 0.4748305794534957 1103 10-11 0.5288959167020266 1103 12-13 0.36925255724655415 1103 14-15 0.6708848236625684 1103 16-17 0.7232899484142763 1103 18-19 0.8398822224351292 1103 20-21 1.062426484996017 1103 22-23 0.8735155824294836 1103 24-25 0.8781504407187626 1103 26-27 0.9491415551052427 1103 28-29 0.9391392948388884 1103 30-31 1.2634153782133026 1103 32-33 1.3631957102123167 1103 34-35 0.8725520175752663 1103 36-37 1.1711160936394904 1103 38-39 1.6169922450319234 1103 40-41 1.5845105627562859 1103 42-43 0.852379685618935 1103 44-45 0.9653798931558057 1103 46-47 1.0250424809919494 1103 48-49 1.1966590491996456 1103 50-51 0.9454853248440429 1103 52-53 1.2238626353211686 1103 54-55 1.0728636743541031 1103 56-57 1.070004300788085 1103 58-59 1.0270908314915168 1103 60-61 0.9850523648164007 1103 62-63 0.9631251456908139 1103 64-65 0.8359371926007952 1103 66-67 0.41122704741539096 1103 68-69 0.5115925374297419 1103 70-71 0.8215534311421671 1103 72-73 0.4719161961284115 1103 74-75 0.812143880980539 1103 76-77 0.38990617109961434 1103 78-79 0.4905785386980632 1103 80-81 0.37602038406300053 1103 82-83 0.5311792425767372 1103 84-85 0.8056846643626301 1103 86-87 0.8424331104454268 1103 88-89 0.912223556447497 1103 90-91 1.0116232648352224 1103 92-93 0.722750557658312 1103 94-95 0.8090252777678621 1103 96-97 0.5562108501875755 1103 98-99 0.3458628801053827 1103 100-101 0.07121719999343412 1104 1 0.3419284692561533 1104 2 0.5044899981423256 1104 3 0.5494703330487276 1104 4 0.4116781570534016 1104 5 0.30347169861522616 1104 6 0.2850557760537953 1104 7 0.38655489861015013 1104 8 0.5602375293253132 1104 9 0.6526332421385774 1104 10-11 0.4534279915700452 1104 12-13 0.3628358061489507 1104 14-15 0.34668780629645823 1104 16-17 0.3892411739959627 1104 18-19 0.2780032341843608 1104 20-21 0.3937556945447156 1104 22-23 0.43031093961678124 1104 24-25 0.5868992631293963 1104 26-27 0.7724142759143575 1104 28-29 0.572055805974891 1104 30-31 0.7699298914467363 1104 32-33 1.0777302735710705 1104 34-35 1.181515916684745 1104 36-37 1.1477909367756993 1104 38-39 1.2498154377655197 1104 40-41 1.2621488073038307 1104 42-43 1.3318629682937058 1104 44-45 1.4326606837848601 1104 46-47 1.6111309290624902 1104 48-49 1.4548888435436638 1104 50-51 1.5938526971755849 1104 52-53 1.5982871718397718 1104 54-55 1.2733027152186693 1104 56-57 1.6064340275873548 1104 58-59 1.6680238377660679 1104 60-61 1.8055744146217627 1104 62-63 1.434000916678567 1104 64-65 1.5201921562510563 1104 66-67 1.3975136859976658 1104 68-69 1.161230817527148 1104 70-71 1.2596922178251866 1104 72-73 1.0933189939029901 1104 74-75 1.161239136850707 1104 76-77 1.2460225258161906 1104 78-79 1.077129135047091 1104 80-81 1.055911601468214 1104 82-83 0.9167619937809874 1104 84-85 1.0995354260185408 1104 86-87 0.9063428848815143 1104 88-89 0.5874355225329246 1104 90-91 0.729828352021272 1104 92-93 0.49022776130572865 1104 94-95 0.5657664214071012 1104 96-97 0.7698788855241432 1104 98-99 0.36221581919068413 1104 100-101 0.5784604855121955 1105 1 0.5313819881107662 1105 2 0.47081385375837925 1105 3 0.5238603310321928 1105 4 0.6551748297696776 1105 5 0.5730825072459993 1105 6 0.6181874558279432 1105 7 0.5865448159321929 1105 8 0.7687977229127299 1105 9 0.7980758717009877 1105 10-11 0.9048042771114808 1105 12-13 0.8709775686010559 1105 14-15 0.8575266851026697 1105 16-17 0.9623153825057429 1105 18-19 0.9424265050050948 1105 20-21 1.0042870516731668 1105 22-23 1.0366579853921323 1105 24-25 1.059726446029174 1105 26-27 0.8603100210242545 1105 28-29 1.0786599600382019 1105 30-31 1.0311720773713162 1105 32-33 0.7811987147890562 1105 34-35 0.9837693952086397 1105 36-37 0.9639837175782802 1105 38-39 1.3697740987858964 1105 40-41 1.177303072276608 1105 42-43 1.0186445774891126 1105 44-45 1.5995290040107122 1105 46-47 1.479375534829778 1105 48-49 1.3856460526584016 1105 50-51 1.1622384604343097 1105 52-53 1.2132549072702972 1105 54-55 0.8901609527665713 1105 56-57 0.888244876548832 1105 58-59 0.8445715288328124 1105 60-61 1.213998492056728 1105 62-63 1.1428887972996584 1105 64-65 0.9291707809737844 1105 66-67 0.9692267329829427 1105 68-69 0.7876423924414446 1105 70-71 0.48667850538316415 1105 72-73 0.5725738840018018 1105 74-75 0.7474712400973296 1105 76-77 0.5130370448724548 1105 78-79 0.43408114150000543 1105 80-81 0.7076811114500678 1105 82-83 0.576496819350659 1105 84-85 0.8835896708259625 1105 86-87 0.6246580693945241 1105 88-89 0.5130253591935379 1105 90-91 0.7965252667218152 1105 92-93 0.5821817842942352 1105 94-95 0.7015548868235157 1105 96-97 0.7790037090772799 1105 98-99 0.8469153553874982 1105 100-101 0.7036369323764831 1106 1 0.05183405258132012 1106 2 0.3743648715455734 1106 3 0.1441647039853784 1106 4 0.34919653400135786 1106 5 0.26957643905105044 1106 6 0.2668421787458115 1106 7 0.19267401390303718 1106 8 0.146486671734408 1106 9 0.30711395042698797 1106 10-11 0.2012227578152661 1106 12-13 0.5319854026728521 1106 14-15 0.5065345833881665 1106 16-17 0.5437463848816506 1106 18-19 0.43373357493381093 1106 20-21 0.7170511305275937 1106 22-23 0.6151993711238504 1106 24-25 0.6579894653575025 1106 26-27 0.6310751272979402 1106 28-29 0.7302364064518727 1106 30-31 0.7436712747000271 1106 32-33 0.9827173688698352 1106 34-35 1.113055659897526 1106 36-37 0.7206779961373257 1106 38-39 0.6669149836507273 1106 40-41 0.6334611664138947 1106 42-43 0.9659411805842097 1106 44-45 0.9005949514839031 1106 46-47 0.9432473000644777 1106 48-49 0.9895518511558024 1106 50-51 1.095143167298751 1106 52-53 0.9004940391010336 1106 54-55 0.8187713682292284 1106 56-57 1.152580585789181 1106 58-59 0.8966167352798671 1106 60-61 0.9341150399280416 1106 62-63 1.2552708046200216 1106 64-65 1.3010082560909737 1106 66-67 0.8213024409390215 1106 68-69 0.601109611345958 1106 70-71 1.004505834735248 1106 72-73 1.1396370702857546 1106 74-75 1.584023285303779 1106 76-77 1.6569800873177094 1106 78-79 1.8315559376214985 1106 80-81 1.442342063097147 1106 82-83 1.4430206105276966 1106 84-85 1.4392067645137558 1106 86-87 1.25623997397296 1106 88-89 1.3498583393515027 1106 90-91 1.0060706337031284 1106 92-93 1.2416244093504183 1106 94-95 1.1519257209595217 1106 96-97 1.0831037540268724 1106 98-99 0.5995561439317747 1106 100-101 0.5747279231939224 1107 1 0.1333461159369591 1107 2 0.10530292790262408 1107 3 0.3955920475914958 1107 4 0.25334964346173194 1107 5 0.3163564746579013 1107 6 0.1762902725514479 1107 7 0.25245361977203373 1107 8 0.3197324203991485 1107 9 0.41614458459729065 1107 10-11 0.2770254467464497 1107 12-13 0.28835473166787295 1107 14-15 0.2299215316354477 1107 16-17 0.2758969159148634 1107 18-19 0.4400108172445769 1107 20-21 0.21025206367266236 1107 22-23 0.37728574843369245 1107 24-25 0.3561139665546307 1107 26-27 0.1789755508966877 1107 28-29 0.30537146692213213 1107 30-31 0.3606213637171578 1107 32-33 0.31067642099090165 1107 34-35 0.6270528359058787 1107 36-37 0.5327597691129498 1107 38-39 0.2912089857380238 1107 40-41 0.2975980072267177 1107 42-43 0.6134563748871074 1107 44-45 0.7364811323421563 1107 46-47 0.8269187961661402 1107 48-49 0.663165946557605 1107 50-51 0.8857764167308773 1107 52-53 0.8117454971402012 1107 54-55 1.0025456944616522 1107 56-57 0.9803094211995855 1107 58-59 0.9077899196374126 1107 60-61 0.5326324443113712 1107 62-63 0.9345712527752177 1107 64-65 1.043150192366987 1107 66-67 0.7880975173709679 1107 68-69 0.8053732248274486 1107 70-71 0.880940852231717 1107 72-73 0.7907436452750112 1107 74-75 0.7666388540925304 1107 76-77 0.6417474149621363 1107 78-79 0.33757994155052984 1107 80-81 0.21980691667932106 1107 82-83 0.4840375544776023 1107 84-85 0.39061569078301517 1107 86-87 0.722927615939934 1107 88-89 1.1686240708267341 1107 90-91 1.2452274266308727 1107 92-93 0.9806123766903418 1107 94-95 0.8160395401012686 1107 96-97 0.9630380655254243 1107 98-99 0.38046657428354536 1107 100-101 0.1944636344100843 1108 1 0.25982258064871644 1108 2 0.2072451033742091 1108 3 0.4045536742001765 1108 4 0.47922670757409236 1108 5 0.4925920038346021 1108 6 0.3304008678385131 1108 7 0.2185904241441321 1108 8 0.48204070035448865 1108 9 0.2558636890222772 1108 10-11 0.18182652801812083 1108 12-13 0.4179457289059343 1108 14-15 0.515004096129779 1108 16-17 0.5098982087396848 1108 18-19 0.32747629294534164 1108 20-21 0.45920898910016916 1108 22-23 0.36180857854476045 1108 24-25 0.22299708343774682 1108 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-1.1355313559735443 2316 92-93 -1.2838214556965077 2316 94-95 -1.4326636582663568 2316 96-97 -1.1980849278123316 2316 98-99 -1.2573139896715126 2316 100-101 -1.480097542667874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2488.0 3 487.0 4 420.0 5 382.0 6 444.0 7 545.0 8 679.0 9 917.0 10 1157.0 11 1794.0 12 2694.0 13 4017.0 14 4177.0 15 5315.0 16 7020.0 17 9190.0 18 7078.0 19 4460.0 20 4314.0 21 4109.0 22 4419.0 23 4638.0 24 5192.0 25 5440.0 26 6002.0 27 6557.0 28 7335.0 29 8212.0 30 9230.0 31 10739.0 32 12518.0 33 15621.0 34 20998.0 35 37371.0 36 38878.0 37 56156.0 38 51233.0 39 8122.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.60675246468481 21.052375059907884 15.132257653993724 15.208614821413583 2 13.990847387072272 25.958442920510667 39.79521501612384 20.255494676293218 3 20.902180025087592 33.90609455426273 31.098068688092045 14.093656732557635 4 10.331421959442105 19.496554390347427 39.95176957536139 30.22025407484908 5 9.30451991759356 44.364297030880785 32.3272249468738 14.003958104651854 6 21.501922250830095 41.171830042227384 19.289510156995075 18.03673754994745 7 21.666518058584007 42.41139580817128 20.46522435767534 15.456861775569372 8 23.72142164240608 32.066396634232156 21.050100113759495 23.162081609602275 9 27.425072684629768 20.57839672297699 22.66922819190904 29.327302400484207 10-11 21.61667060691006 30.101117903340203 26.00425546286602 22.277956026883718 12-13 28.014034443019863 30.072185241678028 26.43509205205768 15.47868826324443 14-15 24.40518326785801 29.337582474588906 31.26941969231102 14.987814565242061 16-17 15.134014211991028 33.882359298586906 32.13193374942585 18.851692739996217 18-19 14.04612102682715 32.95563394457857 38.025731474513734 14.972513554080544 20-21 16.65986613307248 30.907209196122885 36.022876814864254 16.41004785594038 22-23 15.736094108490681 30.719839152958727 35.26230877119022 18.281757967360374 24-25 15.917287939536198 31.541743185592686 37.11394259290039 15.42702628197072 26-27 17.286094395894114 29.806989355563633 38.05186679657464 14.855049451967606 28-29 14.71714940105803 30.667315267108524 39.610839734884564 15.004695596948874 30-31 14.335456934582721 33.766611043176894 37.815550706328246 14.082381315912146 32-33 14.127869193552092 33.815985202939416 36.246313232371406 15.809832371137084 34-35 15.339069757370375 31.25169169472624 36.44370581293375 16.965532734969628 36-37 16.590173975140214 31.289914753671855 37.19455431658624 14.92535695460169 38-39 17.46569960876963 30.374676232484042 37.64007347761534 14.51955068113099 40-41 14.68919819473265 31.490727145651263 39.266461145033624 14.553613514582464 42-43 13.839367364882898 33.41100825205692 37.10688926853258 15.642735114527603 44-45 14.603488176728508 33.80553252144882 35.01319718821304 16.577782113609636 46-47 14.955033814408964 32.833919829159655 33.09625080792792 19.11479554850345 48-49 16.09542446105561 32.15222705861308 33.91433264130181 17.838015839029495 50-51 17.483949299026794 32.69369923601644 34.27288766270898 15.549463802247775 52-53 15.026559705133069 33.20803295571576 35.68337037237791 16.082036966773266 54-55 13.910890954960422 32.11762697442762 36.24858074253925 17.72290132807271 56-57 15.771942538622524 34.30452362730025 32.77655489105772 17.1469789430195 58-59 15.180396209370391 32.14897602346864 34.15778759851398 18.512840168646992 60-61 15.200542596094879 31.080142580391 34.93544487329271 18.78386995022141 62-63 18.27993844052954 30.641280610392496 33.92138161994613 17.157399329131827 64-65 17.255846855612685 30.89408308240211 35.41477508341035 16.435294978574856 66-67 15.100504691845243 31.29929690083429 35.36637592224168 18.233822485078793 68-69 14.632585954040374 33.19413156803266 34.824161197541805 17.34912128038516 70-71 15.426717197182867 32.74981972750389 32.98254745368488 18.840915621628362 72-73 15.520351259968967 31.006010177323944 32.7875161773375 20.686122385369586 74-75 16.13082468121208 31.738795158516435 33.507934292215594 18.622445868055884 76-77 17.581190651693188 29.546649395135066 34.2565743832112 18.615585569960544 78-79 16.064072048541732 29.60311256636353 35.535358434179706 18.797456950915034 80-81 16.099119858787255 32.05437178346115 33.70682099279712 18.139687364954476 82-83 15.935979145666005 31.24369980164537 32.88107393316641 19.939247119522214 84-85 16.51195404012005 29.11027254804989 32.897491311387675 21.48028210044239 86-87 17.878608049079222 29.20880347716754 33.45486619191624 19.457722281837004 88-89 19.13919686921549 27.34734308812649 33.96787368455286 19.54558635810516 90-91 17.35737144080903 27.94165685109105 35.26897839850469 19.431993309595228 92-93 17.381274447881115 29.90997487103446 33.82204246713852 18.886708213945898 94-95 17.630076997830074 29.50842557667051 32.7607401875543 20.100757237945118 96-97 17.735131940641622 27.736270528467404 31.992149165848872 22.5364483650421 98-99 18.818238986891608 27.3933113595212 32.268570220424806 21.519879433162387 100-101 20.45339067447655 26.782460005501065 33.32100336161098 19.443145958411414 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6061.0 1 3740.0 2 1795.0 3 2111.5 4 1672.0 5 1627.0 6 2850.0 7 5260.0 8 7507.5 9 7355.5 10 5201.0 11 3578.0 12 3170.0 13 2902.0 14 2627.0 15 2413.0 16 2190.0 17 2088.5 18 2002.0 19 1996.5 20 2109.0 21 2255.5 22 2469.5 23 2690.0 24 2865.5 25 3278.5 26 4039.5 27 4744.0 28 4986.0 29 5178.5 30 5851.0 31 6534.5 32 7723.0 33 9346.0 34 10732.0 35 11314.0 36 11874.0 37 13187.5 38 12755.0 39 10980.0 40 9992.5 41 9314.0 42 8364.5 43 8084.5 44 8363.0 45 7990.5 46 8671.5 47 11762.5 48 29625.5 49 30193.0 50 11661.0 51 6232.5 52 3408.0 53 2383.0 54 1816.5 55 1446.5 56 1160.0 57 970.0 58 901.5 59 870.5 60 833.0 61 820.5 62 746.0 63 617.5 64 521.0 65 492.5 66 440.0 67 396.5 68 380.0 69 317.0 70 280.0 71 254.0 72 208.0 73 186.5 74 159.0 75 114.5 76 85.0 77 69.0 78 53.0 79 37.5 80 28.0 81 23.0 82 12.5 83 6.5 84 7.5 85 6.5 86 3.0 87 4.0 88 4.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27892534089374915 2 0.10827595517753476 3 0.12042662346429055 4 0.12393681652490887 5 0.1285270689887944 6 0.05697313352234373 7 0.06723369785338193 8 0.07317402457135143 9 0.06966383151073309 10-11 0.06412852706898879 12-13 0.07033886863777508 14-15 0.0637235047927636 16-17 0.06480356419603078 18-19 0.06979883893614149 20-21 0.07587417307951938 22-23 0.08343458890238963 24-25 0.09275010125556905 26-27 0.09302011610638584 28-29 0.08707978938841636 30-31 0.08167949237208046 32-33 0.07465910625084379 34-35 0.24247333603348184 36-37 0.3673552045362495 38-39 0.339678682327528 40-41 0.32617793978668824 42-43 0.34845416497907383 44-45 0.3596597812879708 46-47 0.3646550560280815 48-49 0.3747806129337114 50-51 0.36803024166329146 52-53 0.36992034561900905 54-55 0.3561495882273525 56-57 0.33279330363169973 58-59 0.3176724719859592 60-61 0.2739300661536384 62-63 0.3445389496422303 64-65 0.3755906574861617 66-67 0.38126096935331444 68-69 0.3944916970433374 70-71 0.3953017415957878 72-73 0.37599567976238696 74-75 0.3816659916295396 76-77 0.3623599297961388 78-79 0.341703793708654 80-81 0.3406237343053868 82-83 0.35479951397326853 84-85 0.3597947887133792 86-87 0.35479951397326853 88-89 0.36965033076819226 90-91 0.39516673417037934 92-93 0.392331578236803 94-95 0.38976643715404347 96-97 0.3974618604023221 98-99 0.38504117726474957 100-101 0.35925475901174564 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 370350.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.133877771968752 #Duplication Level Percentage of deduplicated Percentage of total 1 76.97396982630052 20.88602288890049 2 8.876996520228515 4.817346771241449 3 3.706037263009266 3.016774863385651 4 2.087999658226266 2.266221100568961 5 1.4579994357403616 1.9780589240489188 6 0.9859996135968783 1.6052395799127668 7 0.7629997009068928 1.4492198437119443 8 0.5869997698968581 1.2742064006844105 9 0.46999981576066313 1.14776257983279 >10 3.499998628004934 18.546812203146715 >50 0.3469998639764892 6.5319358355353945 >100 0.20699991885629182 10.206132784357095 >500 0.009999996080014097 1.9701900927106095 >1k 0.019999992160028193 10.05391178972679 >5k 0.0059999976480084585 11.285246680337 >10k+ 9.999996080014097E-4 2.9649176618990265 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10927 2.950452274875118 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8122 2.1930606183340084 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7896 2.1320372620494124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7317 1.9756986634264884 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6646 1.794518698528419 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6471 1.7472660996354799 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5139 1.3876063183475091 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3332 0.8996894829215608 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3027 0.8173349534224381 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2771 0.7482111516133387 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2703 0.7298501417577967 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2578 0.6960982854056973 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2307 0.6229242608343459 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2003 0.5408397461860402 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1901 0.5132982314027271 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1677 0.4528149048197651 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1663 0.44903469690833 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1596 0.4309437019036047 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1506 0.4066423653300931 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1460 0.3942216821925206 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1397 0.3772107465910625 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1295 0.3496692318077494 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1253 0.33832860807344406 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1235 0.3334683407587417 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1209 0.32644795463750503 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1120 0.30241663291481036 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1020 0.2754151478331308 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 937 0.2530039152153368 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 916 0.24733360334818413 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 878 0.23707303901714596 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 806 0.21763196975833674 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 790 0.213311732145268 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 778 0.21007155393546645 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 564 0.15228837586067231 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 561 0.15147833130822194 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 526 0.14202781152963415 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 505 0.13635749966248142 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 468 0.12636695018226002 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 455 0.1228567571216417 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 450 0.12150668286755771 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 448 0.12096665316592413 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 438 0.11826650465775618 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 436 0.1177264749561226 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 401 0.10827595517753476 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 399 0.10773592547590118 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 399 0.10773592547590118 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 392 0.10584582152018361 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 390 0.10530579181855002 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.3500742540839745E-4 0.0 12-13 0.0 0.0 0.0 2.700148508167949E-4 0.0 14-15 0.0 0.0 0.0 4.0502227622519235E-4 0.0 16-17 0.0 0.0 0.0 6.750371270419872E-4 0.0 18-19 0.0 0.0 0.0 0.0010800594032671796 0.0 20-21 0.0 0.0 0.0 0.002295126231942757 0.0 22-23 0.0 0.0 0.0 0.00688537869582827 0.0 24-25 0.0 0.0 0.0 0.015795868772782502 0.0 26-27 0.0 0.0 0.0 0.026866477656271093 0.0 28-29 0.0 0.0 0.0 0.053327933036317 0.0 30-31 0.0 0.0 0.0 0.1224517348454165 0.0 32-33 0.0 0.0 0.0 0.21560685837721075 0.0 34-35 0.0 0.0 0.0 0.36951532334278386 0.0 36-37 0.0 0.0 0.0 0.5921425678412313 0.0 38-39 0.0 0.0 0.0 0.8478466315647362 0.0 40-41 0.0 0.0 0.0 1.1190765492102066 0.0 42-43 0.0 0.0 0.0 1.3741055758066694 0.0 44-45 0.0 0.0 0.0 1.646415552855407 0.0 46-47 0.0 0.0 0.0 1.9794788713379234 0.0 48-49 0.0 0.0 0.0 2.348184150128257 0.0 50-51 0.0 0.0 0.0 2.712974213581747 0.0 52-53 0.0 0.0 0.0 3.0361819900094504 0.0 54-55 0.0 0.0 0.0 3.360334818415013 0.0 56-57 0.0 0.0 0.0 3.709194005670312 0.0 58-59 0.0 0.0 0.0 4.155123531794249 0.0 60-61 0.0 0.0 0.0 4.777372755501553 0.0 62-63 0.0 0.0 0.0 5.312137167544215 0.0 64-65 0.0 0.0 0.0 5.7496962332928305 0.0 66-67 0.0 0.0 0.0 6.251383826110436 0.0 68-69 0.0 0.0 0.0 6.806669366815175 0.0 70-71 0.0 0.0 0.0 7.501687592817605 0.0 72-73 0.0 0.0 0.0 8.22492237073039 0.0 74-75 0.0 0.0 0.0 8.93843661401377 0.0 76-77 0.0 0.0 0.0 9.589037397056838 0.0 78-79 0.0 0.0 0.0 10.318887538814636 0.0 80-81 0.0 0.0 0.0 11.112056163088969 0.0 82-83 0.0 0.0 0.0 11.884838666126637 0.0 84-85 0.0 0.0 0.0 12.90968003240178 0.0 86-87 0.0 0.0 0.0 13.981503982719051 0.0 88-89 0.0 0.0 0.0 14.842176319697582 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACG 40 5.437536E-4 47.403717 7 GTACGGC 45 9.7131083E-4 42.13664 9 GGTACTC 60 8.352771E-5 39.524464 3 CCTTTAG 125 1.3460522E-10 37.984573 1 CATATAG 105 4.6595233E-8 36.17578 1 CTTTAGG 135 3.1286618E-10 35.132854 2 GGATATG 190 0.0 34.985786 1 TGGGAGT 95 8.8175875E-7 34.929054 6 CTTATGT 55 0.0025962538 34.494076 2 ATAGTAC 55 0.0026031483 34.47543 6 GATTGGC 195 0.0 34.033436 9 GCTTATG 85 1.6337532E-5 33.515797 1 CATGGGT 245 0.0 32.910164 4 ATATGAG 205 0.0 32.390778 3 TCCCGCT 60 0.003986924 31.602478 8 TGGGCAG 60 0.003986924 31.602478 6 AGTACGG 60 0.003986924 31.602478 8 ATGAGAT 215 0.0 30.88423 5 CATGGGG 1025 0.0 30.539877 4 ATATAAC 80 4.5270997E-4 29.643349 3 >>END_MODULE