FastQCFastQC Report
Fri 27 May 2016
SRR939354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939354_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370350
Sequences flagged as poor quality0
Sequence length101
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106722.8815984879168357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84942.293506142837856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78962.1320372620494124No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA77492.092345078979344No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA68581.8517618469015795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68231.8423113271229918No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT46361.2517888483866613No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG35790.9663831510733091No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.8049142702848656No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT28350.7654921020656136No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA27310.737410557580667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27260.7360604833265829No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC25690.6936681517483462No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18770.5068178749831241No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG18590.5019576076684218No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18040.487106790873498No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG17040.4601053057918186No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT16510.44579451869852843No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG15210.41069258809234505No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA14900.40232212771702447No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14160.3823410287565816No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.3820710139057648No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG13010.3512893209126502No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA12930.34912920210611587No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT12280.3315782368030241No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11640.31429728635074927No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9820.2651545835020926No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT9540.25759416767922233No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC9340.25219387066288645No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT8970.24220332118266505No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA8730.23572296476306198No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT7670.2071013905764817No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7280.1965708113946267No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.18982044012420685No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC6100.16470905899824492No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT5800.15660861347374105RNA PCR Primer, Index 33 (95% over 22bp)
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC5740.15498852436884028No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5120.138247603618199No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5020.13554745511003105No Hit
GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT4620.12474686107735926No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.12447684622654245No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT4510.1217766977183745No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4490.12123666801674092No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.11934656406102336No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4410.11907654921020655No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4300.11610638585122181No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4180.11286620764142027No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.11097610368570271No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4050.10935601458080195No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4010.10827595517753476No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3990.10773592547590118No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3960.1069258809234508No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT3950.106655866072634No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3930.10611583637100042No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.10530579181855002No Hit
TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA3850.10395571756446605No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC3760.1015255839071149No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG1050.054.2449849
CCTTTAG1001.2732926E-1147.5735441
CTTATGT707.948256E-847.4643632
CGAAGAG205.4069026E-447.46436394-95
GGCATTC1250.045.5657847
CTTTAGG1052.0008883E-1145.204152
GCATTCC1300.043.8132558
TAGGCAT1155.4569682E-1141.2733575
GGGCAGT500.001602173638.0588341
AGGGGTC500.001620366137.9714855
GGTTTGC651.3323176E-436.5110448
AGGCATT1302.055458E-1036.5110446
GGGAAGT1107.037124E-834.519537
CATGGGT2300.033.0186844
TTAGGCA1456.657501E-1032.7340434
GTACAGG600.0039177331.7156981
GCTTATG1051.8742903E-631.7156981
GGGACCT600.003962056731.6429049
GTACCCA650.005795032729.2760261
GGGGTCC650.00586048329.2088366
Produced by FastQC (version 0.11.5)