FastQCFastQC Report
Fri 27 May 2016
SRR939353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2591316
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92140.3555722266215313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70750.2730272957833008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47470.18318877358068256No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65700.042.1801761
GTATCAA111000.039.159161
ATCAACG137900.031.2891353
TATCAAC139700.031.0217442
TCAACGC140950.030.612074
CAACGCA141600.030.4045815
AACGCAG145200.029.6684866
ACGCAGA165350.026.0530037
CGCAGAG166900.025.6690718
GTACATG126200.025.4560031
TACATGG128300.024.5020982
GCAGAGT177850.023.5543619
ACATGGG131100.023.0384453
GAGTACT89400.022.31282212-13
GTACTTT95100.020.7552714-15
AGTACTT93150.020.39724312-13
GTCTACG2108.699044E-720.336871
CATGGGG62250.019.7277164
GTATAGG6750.019.684081
AGAGTAC168500.019.47399310-11
Produced by FastQC (version 0.11.5)