##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939353_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2591316 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.878501502711362 33.0 31.0 34.0 30.0 34.0 2 31.935529283190473 33.0 31.0 34.0 30.0 34.0 3 31.982273871654403 34.0 31.0 34.0 30.0 34.0 4 35.47690208372889 37.0 35.0 37.0 33.0 37.0 5 35.51039394655071 37.0 35.0 37.0 33.0 37.0 6 35.60626839798774 37.0 35.0 37.0 33.0 37.0 7 35.53962851307984 37.0 35.0 37.0 33.0 37.0 8 35.52733475963564 37.0 35.0 37.0 33.0 37.0 9 37.14878540479046 39.0 37.0 39.0 34.0 39.0 10-11 37.04431763628982 39.0 37.0 39.0 33.0 39.0 12-13 36.93878728028538 39.0 37.0 39.0 33.0 39.0 14-15 38.16161672293151 40.0 38.0 41.0 33.0 41.0 16-17 38.081708097352845 40.0 38.0 41.0 33.0 41.0 18-19 38.01312344769993 40.0 38.0 41.0 33.0 41.0 20-21 37.992785326065984 40.0 38.0 41.0 33.0 41.0 22-23 37.99695116303839 40.0 38.0 41.0 33.0 41.0 24-25 37.91350591745662 40.0 38.0 41.0 33.0 41.0 26-27 37.82955552313959 40.0 38.0 41.0 33.0 41.0 28-29 37.68044345035496 40.0 38.0 41.0 32.0 41.0 30-31 37.487384209413285 40.0 37.0 41.0 32.0 41.0 32-33 37.269103806714426 40.0 37.0 41.0 31.5 41.0 34-35 36.98514249130558 40.0 36.0 41.0 30.5 41.0 36-37 36.750988879781545 40.0 36.0 41.0 30.0 41.0 38-39 36.1609469860102 38.5 35.5 40.5 29.0 41.0 40-41 36.13108744745913 39.0 35.0 40.0 30.0 41.0 42-43 36.30063469681042 39.0 35.5 41.0 30.0 41.0 44-45 36.19418762512947 40.0 35.5 41.0 29.5 41.0 46-47 35.903020704537774 39.0 35.0 41.0 27.5 41.0 48-49 35.65212019684206 39.0 35.0 41.0 27.0 41.0 50-51 34.521187689961394 38.0 34.0 40.0 25.0 40.5 52-53 34.62672865833422 38.0 34.5 39.5 25.0 40.5 54-55 35.1112569829384 39.0 35.0 40.0 26.0 41.0 56-57 35.03242792465296 39.0 35.0 41.0 26.0 41.0 58-59 34.819893058199 38.5 34.5 40.0 25.0 41.0 60-61 34.52265798536342 38.0 34.0 40.0 24.5 41.0 62-63 34.133535045513554 37.5 34.0 40.0 23.0 41.0 64-65 33.740045212548374 37.0 34.0 40.0 22.0 41.0 66-67 33.380207971548046 36.5 33.0 39.5 22.0 41.0 68-69 32.97300252072692 36.0 33.0 39.0 20.5 41.0 70-71 32.56225524019456 35.5 32.0 39.0 19.5 40.0 72-73 32.114095502053786 35.0 32.0 38.0 18.0 40.0 74-75 31.64917613289927 35.0 31.5 37.0 15.5 39.0 76-77 31.182017361062876 35.0 31.0 37.0 11.5 39.0 78-79 30.785541786489954 35.0 31.0 36.0 7.5 39.0 80-81 30.37996639545312 34.5 30.5 36.0 6.0 37.5 82-83 29.953646911453482 34.0 30.0 35.0 2.0 37.0 84-85 29.53559253290606 34.0 30.0 35.0 2.0 36.5 86-87 29.10421210689858 34.0 29.0 35.0 2.0 36.0 88-89 28.67811856986952 34.0 29.0 35.0 2.0 36.0 90-91 28.27114639820076 34.0 29.0 35.0 2.0 35.5 92-93 27.82819212323005 34.0 27.5 35.0 2.0 35.0 94-95 27.34297920438881 34.0 26.5 35.0 2.0 35.0 96-97 26.730376959043205 33.0 25.5 35.0 2.0 35.0 98-99 25.931160653505785 33.0 24.5 35.0 2.0 35.0 100-101 23.202551136179455 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40813152498416727 1101 2 0.42111939692165024 1101 3 0.4888132422409015 1101 4 0.3847627006472507 1101 5 0.32038001921671366 1101 6 0.38686135353727025 1101 7 0.3901818229085663 1101 8 0.2387706237915026 1101 9 0.40086252370220166 1101 10-11 0.44899128459278614 1101 12-13 0.5655876921557592 1101 14-15 0.7352368002409193 1101 16-17 0.6197539047153171 1101 18-19 0.6238842332974812 1101 20-21 0.6965432393302535 1101 22-23 0.7490068464468322 1101 24-25 0.6624855254791697 1101 26-27 0.7424097388777255 1101 28-29 0.6464976886322944 1101 30-31 0.6836878501440182 1101 32-33 0.7955994357523295 1101 34-35 0.7120445795616561 1101 36-37 0.6968887409422706 1101 38-39 1.0228420647944674 1101 40-41 0.9358841984123814 1101 42-43 0.8070263448698398 1101 44-45 0.5865166906642258 1101 46-47 0.6892981035596009 1101 48-49 0.8389192999737176 1101 50-51 0.8061014079110649 1101 52-53 0.7968787594270097 1101 54-55 0.7322580788366722 1101 56-57 0.8061239123058854 1101 58-59 0.7657020928513347 1101 60-61 0.7719826409246693 1101 62-63 0.8848026921578764 1101 64-65 0.8156807044448442 1101 66-67 0.8161613562603165 1101 68-69 0.8060893351402498 1101 70-71 0.936450451112556 1101 72-73 0.7819731710471487 1101 74-75 0.7265065135010538 1101 76-77 0.9014331454185225 1101 78-79 0.8022453243950451 1101 80-81 0.6133938253846871 1101 82-83 0.5951725429251731 1101 84-85 0.681136487688331 1101 86-87 0.7466349915108381 1101 88-89 0.6153077225469836 1101 90-91 0.750661847327823 1101 92-93 0.7315189766582506 1101 94-95 0.7032913493399882 1101 96-97 0.903382703355657 1101 98-99 0.9735801663974009 1101 100-101 0.6851849318582595 1102 1 0.2181008478754869 1102 2 0.21225032033212088 1102 3 0.23794454911522678 1102 4 0.1844876390718042 1102 5 0.14928729470599933 1102 6 0.2545703782116888 1102 7 0.15689089182881588 1102 8 0.1921952510203795 1102 9 0.20462268180730092 1102 10-11 0.37534151983312114 1102 12-13 0.2614953216116831 1102 14-15 0.42976690952811936 1102 16-17 0.32721633263148675 1102 18-19 0.3413915707309272 1102 20-21 0.4218293623631624 1102 22-23 0.3791939325639646 1102 24-25 0.4506016486595641 1102 26-27 0.34237507964435565 1102 28-29 0.33238197841298245 1102 30-31 0.41991467639065405 1102 32-33 0.31481539964773475 1102 34-35 0.17897155919382612 1102 36-37 0.20633854404833585 1102 38-39 0.7812106104340444 1102 40-41 0.6176862573183328 1102 42-43 0.3941537581111376 1102 44-45 0.4749119312878065 1102 46-47 0.4815344352853401 1102 48-49 0.49103793779209326 1102 50-51 0.4116905780067839 1102 52-53 0.39438605998892484 1102 54-55 0.4641501042632754 1102 56-57 0.4282624976192224 1102 58-59 0.5087521791307097 1102 60-61 0.42821300683767305 1102 62-63 0.39131918110378905 1102 64-65 0.3166404702369192 1102 66-67 0.47037614026692864 1102 68-69 0.41999653688648664 1102 70-71 0.5163995315367202 1102 72-73 0.482969070933585 1102 74-75 0.3896840277703326 1102 76-77 0.45847173515643647 1102 78-79 0.4035374869837227 1102 80-81 0.3103775502936159 1102 82-83 0.2015625625408255 1102 84-85 0.3209542317409557 1102 86-87 0.0468023882336972 1102 88-89 0.10357741562841127 1102 90-91 0.18908013265442847 1102 92-93 0.1031787852212922 1102 94-95 0.030472668750633147 1102 96-97 0.07407371260303464 1102 98-99 0.15524672244949045 1102 100-101 -0.09172316955318038 1103 1 0.32775825455422236 1103 2 0.2966329440828126 1103 3 0.3434834909058395 1103 4 0.35777576547631895 1103 5 0.2806234786116306 1103 6 0.27686452054793875 1103 7 0.2949392553729737 1103 8 0.32400580163552917 1103 9 0.37747360387125894 1103 10-11 0.400387863493215 1103 12-13 0.3946710427802458 1103 14-15 0.5188986654281891 1103 16-17 0.5020204650869147 1103 18-19 0.5592111452436654 1103 20-21 0.6028090061290641 1103 22-23 0.5804073932112104 1103 24-25 0.48636988226658673 1103 26-27 0.5526004147614856 1103 28-29 0.5165821786525697 1103 30-31 0.5337282568318713 1103 32-33 0.47507920874682696 1103 34-35 0.39775954132090874 1103 36-37 0.2728056269335326 1103 38-39 0.8350780107295961 1103 40-41 0.6817312281568064 1103 42-43 0.6114552454803075 1103 44-45 0.6704532121216431 1103 46-47 0.6551307907499506 1103 48-49 0.589830816607801 1103 50-51 0.4838527939332664 1103 52-53 0.5261162743064958 1103 54-55 0.4910261480886362 1103 56-57 0.4111956796798495 1103 58-59 0.47414636256416287 1103 60-61 0.5332803144459106 1103 62-63 0.454401755887595 1103 64-65 0.4837126439294579 1103 66-67 0.49675158512234674 1103 68-69 0.4948240597741318 1103 70-71 0.32249776697999977 1103 72-73 0.3810578321588025 1103 74-75 0.40195963264254075 1103 76-77 0.44723394408764605 1103 78-79 0.3896863069692067 1103 80-81 0.4795543163805469 1103 82-83 0.43021770012903104 1103 84-85 0.4525784240059352 1103 86-87 0.2593339762473015 1103 88-89 0.16993629043932046 1103 90-91 0.31812208678295306 1103 92-93 0.3811642350608402 1103 94-95 0.34578771819844434 1103 96-97 0.13953139996695185 1103 98-99 0.07965463847127907 1103 100-101 -0.29022268425636355 1104 1 0.20308905396933952 1104 2 0.22445765806201834 1104 3 0.2093131581221641 1104 4 0.15547649289901955 1104 5 0.19958407696154268 1104 6 0.1596283589459233 1104 7 0.28319424026637563 1104 8 0.2706863475527186 1104 9 0.3715781057143914 1104 10-11 0.3182682571772375 1104 12-13 0.32852318561060656 1104 14-15 0.2957258701740173 1104 16-17 0.25275386534058697 1104 18-19 0.31231593782951705 1104 20-21 0.3734209664776955 1104 22-23 0.45706846431866666 1104 24-25 0.5836834479252744 1104 26-27 0.48325350353138674 1104 28-29 0.426892473986328 1104 30-31 0.41499487638548516 1104 32-33 0.45094473423067427 1104 34-35 0.39377756878371883 1104 36-37 0.24214694967845674 1104 38-39 0.8517048152266966 1104 40-41 0.6598222440655945 1104 42-43 0.5266639541794618 1104 44-45 0.40187945720430207 1104 46-47 0.4626133125857237 1104 48-49 0.35622771506927364 1104 50-51 0.2998069994805803 1104 52-53 0.4149363682354945 1104 54-55 0.4996819334794935 1104 56-57 0.4216312914881044 1104 58-59 0.29952176375019235 1104 60-61 0.44110444128046566 1104 62-63 0.4428539294192575 1104 64-65 0.43704543256233563 1104 66-67 0.5086515215267937 1104 68-69 0.34758940494091206 1104 70-71 0.43076670893827185 1104 72-73 0.27374653726434417 1104 74-75 0.37024020430920146 1104 76-77 0.3648533059958261 1104 78-79 0.21676690353646322 1104 80-81 0.12292018318643372 1104 82-83 -0.03409469336257587 1104 84-85 0.10384120943427888 1104 86-87 0.13994200343422492 1104 88-89 0.08277113089514287 1104 90-91 0.038116342669074044 1104 92-93 -0.06481845761279814 1104 94-95 -0.08322065816268776 1104 96-97 0.02200874007438003 1104 98-99 -0.1651901608065991 1104 100-101 -0.2844439076252847 1105 1 0.2354509956037667 1105 2 0.25792667915756695 1105 3 0.3107551483552413 1105 4 0.3257625827490713 1105 5 0.17915841815950273 1105 6 0.2255451523924208 1105 7 0.25427373421394606 1105 8 0.21921219644757173 1105 9 0.300463253615078 1105 10-11 0.3600767924435715 1105 12-13 0.340915123541663 1105 14-15 0.483001398058569 1105 16-17 0.45892856470226207 1105 18-19 0.34612246855537165 1105 20-21 0.4120405144746826 1105 22-23 0.5133091268086929 1105 24-25 0.43359202624375115 1105 26-27 0.49560671505349774 1105 28-29 0.33519996406706554 1105 30-31 0.4826772692227621 1105 32-33 0.4555852351811396 1105 34-35 0.47708046811796834 1105 36-37 0.49375088982127124 1105 38-39 0.8478656004375509 1105 40-41 0.566545114463608 1105 42-43 0.4342802676236559 1105 44-45 0.3278924391856606 1105 46-47 0.479467507399562 1105 48-49 0.4021811729357907 1105 50-51 0.30744148783546876 1105 52-53 0.35004142195491994 1105 54-55 0.47107209358625823 1105 56-57 0.3659003984827649 1105 58-59 0.4414000236503952 1105 60-61 0.4702672224829243 1105 62-63 0.25840414705653814 1105 64-65 0.313109468432053 1105 66-67 0.27909496199122685 1105 68-69 0.2339941613988188 1105 70-71 0.325700337173636 1105 72-73 0.27969527273167927 1105 74-75 0.3171951972119267 1105 76-77 0.3394905002409949 1105 78-79 0.3835104245996952 1105 80-81 0.44867177473070186 1105 82-83 0.43646400305007305 1105 84-85 0.573913816749176 1105 86-87 0.41062079445899613 1105 88-89 0.25421482406435203 1105 90-91 0.2922738321171998 1105 92-93 0.35402415449972935 1105 94-95 0.3284626976199938 1105 96-97 0.3174489234169968 1105 98-99 0.10062979097089553 1105 100-101 -0.059488182254856525 1106 1 0.18306711346774662 1106 2 0.2753118549801563 1106 3 0.26288098810004357 1106 4 0.22586460702513023 1106 5 0.19166491689417597 1106 6 0.21470759821475127 1106 7 0.21260328991785826 1106 8 0.3267654674867799 1106 9 0.25092063363231887 1106 10-11 0.33961545460197584 1106 12-13 0.27309596880301257 1106 14-15 0.38851201800513024 1106 16-17 0.2774570653203412 1106 18-19 0.3700576901701069 1106 20-21 0.41304273774090206 1106 22-23 0.5288381331038821 1106 24-25 0.4158345014079572 1106 26-27 0.4011803628278159 1106 28-29 0.44115758060026167 1106 30-31 0.3550984412074385 1106 32-33 0.36899396918964555 1106 34-35 0.2308179779014239 1106 36-37 0.1770183590746086 1106 38-39 0.6787804428930357 1106 40-41 0.6286936232171172 1106 42-43 0.4964524522150313 1106 44-45 0.3605385914256658 1106 46-47 0.35474561768953095 1106 48-49 0.1953711939175875 1106 50-51 0.15158845709388657 1106 52-53 0.2165913901743508 1106 54-55 0.2039097372957741 1106 56-57 0.2996998468438292 1106 58-59 0.269177777186691 1106 60-61 0.26157893339270544 1106 62-63 0.1724909150428715 1106 64-65 0.3756910914891236 1106 66-67 0.24659345053543547 1106 68-69 0.21643129558489704 1106 70-71 0.18415599701601337 1106 72-73 0.19602122550638512 1106 74-75 0.2650182564436996 1106 76-77 0.329464631357947 1106 78-79 0.4938519497729281 1106 80-81 0.3987369277275228 1106 82-83 0.3767206523646962 1106 84-85 0.5004877710973119 1106 86-87 0.48028343235459303 1106 88-89 0.46824189338510536 1106 90-91 0.41473003302922606 1106 92-93 0.3796515924847874 1106 94-95 0.49440898853737636 1106 96-97 0.38794821532359336 1106 98-99 0.4638468799805757 1106 100-101 0.4763568746584639 1107 1 0.10924508489601337 1107 2 0.02310292551462112 1107 3 0.06367860423072358 1107 4 0.061691131223746254 1107 5 0.0939329713104371 1107 6 0.12761068656127605 1107 7 0.057814016721017936 1107 8 0.13783271469908698 1107 9 0.2603319256608785 1107 10-11 0.3026070401398684 1107 12-13 0.24609542244889582 1107 14-15 0.1747764625273618 1107 16-17 0.23638356319046494 1107 18-19 0.32043998586181743 1107 20-21 0.25519487072867264 1107 22-23 0.15660540147028001 1107 24-25 0.17273127091804952 1107 26-27 0.1927916965558225 1107 28-29 0.27062067734755146 1107 30-31 0.21875090215986148 1107 32-33 0.1948197781057175 1107 34-35 0.24475457343044837 1107 36-37 0.14384302201672483 1107 38-39 0.5013530098290246 1107 40-41 0.15902122262237128 1107 42-43 0.09052428180125105 1107 44-45 0.008935369306087182 1107 46-47 0.026207974804236756 1107 48-49 0.12533453188250832 1107 50-51 0.23216069834803932 1107 52-53 0.20880561577445889 1107 54-55 0.22712194945161457 1107 56-57 0.11924304001728814 1107 58-59 0.2044364127015399 1107 60-61 0.21694096528212725 1107 62-63 0.17740878690989348 1107 64-65 0.16125463521191108 1107 66-67 0.01478597720254271 1107 68-69 0.19378217819789967 1107 70-71 0.24618415784195946 1107 72-73 0.18104686153714766 1107 74-75 0.1838258500049399 1107 76-77 0.29114017310838136 1107 78-79 0.3168939399020303 1107 80-81 0.19287084992609138 1107 82-83 0.1759155790330169 1107 84-85 0.32343685058955174 1107 86-87 0.4039087582266667 1107 88-89 0.41533092171181707 1107 90-91 0.3883414337198552 1107 92-93 0.30367169344615874 1107 94-95 0.4335405746535912 1107 96-97 0.39646106682028304 1107 98-99 0.5974927055459887 1107 100-101 0.37455474311648373 1108 1 0.16584044023614197 1108 2 0.21551191876043774 1108 3 0.2544414205165566 1108 4 0.2710051238013378 1108 5 0.21555973310456267 1108 6 0.1438114822599843 1108 7 0.3080755046394046 1108 8 0.29579095006317857 1108 9 0.35770147909737204 1108 10-11 0.4263665329370241 1108 12-13 0.4013017563163217 1108 14-15 0.45224664585528984 1108 16-17 0.5114832458215304 1108 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2316 78-79 -0.9589747859666957 2316 80-81 -0.8320714073822408 2316 82-83 -0.8517069733663227 2316 84-85 -0.7765390181363188 2316 86-87 -0.6934954061417855 2316 88-89 -0.9480895140300483 2316 90-91 -1.0130564565173223 2316 92-93 -1.1440504866420902 2316 94-95 -0.9713830671004082 2316 96-97 -0.9250847136540692 2316 98-99 -1.2466545986459572 2316 100-101 -0.37691640277430594 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9823.0 3 2397.0 4 2395.0 5 2662.0 6 3224.0 7 3750.0 8 4686.0 9 5663.0 10 6650.0 11 8328.0 12 10460.0 13 13750.0 14 14002.0 15 15237.0 16 18933.0 17 23994.0 18 21120.0 19 15017.0 20 15164.0 21 16049.0 22 17485.0 23 19391.0 24 21629.0 25 24426.0 26 28659.0 27 33387.0 28 39503.0 29 47404.0 30 56886.0 31 70033.0 32 87144.0 33 112567.0 34 154860.0 35 221548.0 36 342687.0 37 538381.0 38 494352.0 39 67652.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.438604774463954 18.934811171247176 14.551453101930957 23.075130952357917 2 15.21031570067798 20.09831987904088 40.78253066987183 23.90883375040931 3 19.163584179728577 24.572304937339947 29.795535239320575 26.468575643610905 4 12.867195275772014 16.433644038763294 37.033584181631596 33.6655765038331 5 13.8645714455873 37.51674999932323 33.82190801296765 14.796770542121825 6 32.06453292784878 35.66108352765377 17.687409117530255 14.586974426967195 7 28.68027816480469 32.98470000842315 20.432818439430225 17.90220338734194 8 26.42399908801611 35.19287879695413 19.723583864840897 18.65953825018887 9 27.011711239969316 16.866542875195396 20.364341100249835 35.757404784585454 10-11 25.15037656447848 26.725819096809396 27.08236814254079 21.041436196171333 12-13 25.903148155338645 24.098754642779113 27.769455048277948 22.228642153604294 14-15 22.898262425139258 25.292743485664086 26.226110591704394 25.582883497492258 16-17 22.27763183310798 28.713675874058335 26.736179254394436 22.27251303843925 18-19 22.14002462321306 27.077438691213622 29.354409649579065 21.428127035994255 20-21 22.971430515115003 26.252806680450274 29.238391871149933 21.537370933284787 22-23 22.678936127075964 26.262635438194415 29.08673475402872 21.9716936807009 24-25 22.313524431309954 26.352103856917974 29.313260938109252 22.02111077366282 26-27 22.080752244460122 26.7192516211332 29.142920649033115 22.057075485373563 28-29 21.97260664760195 26.73859228799008 29.351911767320786 21.93688929708718 30-31 22.20263898318154 26.521516214245533 29.216253650357633 22.0595911522153 32-33 21.96592913657781 26.61691258626578 29.074536609237644 22.342621667918767 34-35 22.508291479811078 26.688428755620674 28.898263750864288 21.90501601370396 36-37 22.48037250561201 26.438845573781933 28.912336340620868 22.168445579985192 38-39 22.195400104838157 26.728051570295342 29.007651635019823 22.06889668984669 40-41 22.400586881432822 26.53080759365049 29.038326721358043 22.030278803558648 42-43 22.336438146740566 26.582969337100792 28.70922933710467 22.371363179053976 44-45 22.29241105823044 26.807360912041382 28.58651610696174 22.313711922766437 46-47 22.33697044784694 27.044117926398926 28.160256069815233 22.458655555938904 48-49 22.49135618861227 26.982997725741303 28.077596186875365 22.448049898771064 50-51 22.50007995805352 27.206827739338685 27.90022397946871 22.392868323139083 52-53 22.68312606908188 27.215490463363157 27.768505293651497 22.33287817390347 54-55 22.283278412480477 27.051837506633337 28.017949641305652 22.646934439580537 56-57 22.351775757645033 27.468944511533206 27.789065437925153 22.3902142928966 58-59 22.469363199729717 26.993317139386182 27.991707107946883 22.545612552937218 60-61 22.514468260447277 27.028267325122258 28.197462322883858 22.2598020915466 62-63 22.44394794424104 27.038022197513516 28.142276384940924 22.37575347330452 64-65 22.249338326142503 26.9008658070754 28.10228797457436 22.747507892207736 66-67 22.181087509862575 27.281748762695337 27.86689257694746 22.67027115049463 68-69 22.21171830271568 27.08926157984421 28.058345093694808 22.6406750237453 70-71 22.215583384820942 27.146878203031715 27.856622049672765 22.780916362474578 72-73 22.308941015367843 27.070925164444986 27.821928738307793 22.798205081879374 74-75 22.255527910670963 27.18926742606738 27.68526628460118 22.869938378660475 76-77 22.274016596553352 27.001078591841658 27.819257855162462 22.905646956442528 78-79 22.263012848078194 27.115376901054933 27.76999926553619 22.851610985330684 80-81 22.15817428630616 27.39388311421795 27.708589169267096 22.739353430208798 82-83 22.29599590989838 27.286156331653284 27.621694283509317 22.79615347493902 84-85 22.304464904871676 27.118578697596856 27.49777020277685 23.079186194754616 86-87 22.331899368709283 26.990682201757192 27.761770901087452 22.91564752844607 88-89 22.358705166602963 26.823359846426158 27.766837261194148 23.051097725776724 90-91 22.25725341491123 27.035376270292694 27.79572956772245 22.911640747073626 92-93 22.402917543453537 26.80910842455988 27.754332170488084 23.0336418614985 94-95 22.28892375075027 26.69396464149065 27.779918947464743 23.23719266029434 96-97 22.431621077487225 26.54093817519854 27.742092452703993 23.285348294610237 98-99 22.250331789827282 26.574533197011625 27.609469318503887 23.565665694657202 100-101 22.536453111168786 26.08939702854981 28.100716783423486 23.273433076857923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3059.0 1 1773.0 2 554.5 3 628.5 4 574.0 5 550.5 6 850.0 7 1636.5 8 2647.0 9 3115.0 10 2924.5 11 2855.5 12 3056.5 13 3322.5 14 3604.0 15 3820.5 16 4034.0 17 4178.5 18 4359.5 19 4621.5 20 4955.0 21 5499.5 22 6340.5 23 7555.5 24 9100.5 25 11269.5 26 13947.0 27 17142.5 28 20649.0 29 24351.5 30 28513.0 31 33487.5 32 39316.0 33 44274.0 34 48330.0 35 52815.0 36 58301.0 37 64529.5 38 69216.0 39 71598.0 40 73931.5 41 79216.0 42 84210.0 43 87860.0 44 92524.0 45 97281.0 46 98963.0 47 99471.0 48 105286.5 49 106183.5 50 101531.5 51 98039.0 52 93620.0 53 91429.5 54 87644.0 55 78872.0 56 69952.0 57 62884.5 58 56243.5 59 48594.0 60 40682.5 61 33533.0 62 27174.0 63 21278.5 64 16590.0 65 13168.0 66 10171.5 67 8338.0 68 6466.0 69 4411.5 70 3246.0 71 2509.0 72 1972.0 73 1643.0 74 1280.0 75 972.5 76 710.0 77 474.0 78 315.0 79 218.5 80 140.5 81 107.0 82 84.5 83 55.0 84 49.5 85 40.0 86 22.5 87 16.0 88 14.5 89 12.5 90 14.5 91 11.0 92 5.5 93 7.0 94 5.0 95 2.5 96 3.5 97 4.0 98 2.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2840641589061311 2 0.17971563483573597 3 0.1798314061272342 4 0.19904944051593862 5 0.2120544155942386 6 0.1083619288423334 7 0.1238752819030948 8 0.1370346187033924 9 0.1401218531433449 10-11 0.12785009624453367 12-13 0.1166395761844561 14-15 0.13226870053671572 16-17 0.10867065228632865 18-19 0.13560677277491437 20-21 0.1615009516400161 22-23 0.16580378464069995 24-25 0.1895368993978349 26-27 0.16945058032289384 28-29 0.1674052875064253 30-31 0.1570437569173347 32-33 0.16692290712518273 34-35 0.3471788079879104 36-37 0.4640885171858623 38-39 0.4302254144226332 40-41 0.42051992115203246 42-43 0.44396360768042187 44-45 0.4479770124523601 46-47 0.4517781698565516 48-49 0.46362543201986944 50-51 0.45685281146722356 52-53 0.46308516599287775 54-55 0.44544933925464897 56-57 0.407997326454975 58-59 0.3978866336641305 60-61 0.3516360027105918 62-63 0.4603066549969205 64-65 0.45818418131945315 66-67 0.46775460808330593 68-69 0.4779231865198995 70-71 0.4714399941959993 72-73 0.4643972406298576 74-75 0.4699156721912727 76-77 0.44624044307988686 78-79 0.43230929766960113 80-81 0.42953078667364375 82-83 0.4410500301777166 84-85 0.4432110942856834 86-87 0.44463894021416145 88-89 0.45189394114804987 90-91 0.47026298606576733 92-93 0.4745465238512015 94-95 0.4726555927567305 96-97 0.47132422290450104 98-99 0.4678317889443047 100-101 0.4502152574213257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2591316.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.957853878255335 #Duplication Level Percentage of deduplicated Percentage of total 1 71.40958744739166 35.67469735203289 2 14.782287661977694 14.769827340068368 3 5.684362051061951 8.519355862141582 4 2.7155884758084534 5.426598890716513 5 1.5103211826310452 3.7726202475557775 6 0.9275826418554763 2.780402284909316 7 0.6026574219281251 2.1075229996331943 8 0.4268079846185255 1.705792874371596 9 0.29715036392114114 1.3360495014578488 >10 1.4814325963836523 13.312141546394654 >50 0.1016891486988154 3.5103341006933584 >100 0.056810484859932224 5.017638609067967 >500 0.0031021157197132277 1.0097398981314156 >1k 4.653173579552067E-4 0.4261813705552709 >5k 1.5510578598506889E-4 0.631097122270437 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9214 0.3555722266215313 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7075 0.2730272957833008 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4747 0.18318877358068256 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.8590430499406476E-5 0.0 0.0 0.0 0.0 7 3.8590430499406476E-5 0.0 0.0 0.0 0.0 8 3.8590430499406476E-5 0.0 0.0 0.0 0.0 9 3.8590430499406476E-5 0.0 0.0 0.0 0.0 10-11 3.8590430499406476E-5 0.0 0.0 0.0 0.0 12-13 7.718086099881295E-5 0.0 0.0 0.0 0.0 14-15 7.718086099881295E-5 0.0 0.0 0.0 0.0 16-17 7.718086099881295E-5 0.0 0.0 0.0 0.0 18-19 7.718086099881295E-5 0.0 0.0 0.0 0.0 20-21 7.718086099881295E-5 0.0 0.0 3.8590430499406476E-5 0.0 22-23 7.718086099881295E-5 0.0 0.0 1.1577129149821944E-4 0.0 24-25 7.718086099881295E-5 0.0 0.0 2.701330134958454E-4 0.0 26-27 7.718086099881295E-5 0.0 0.0 8.682846862366458E-4 0.0 28-29 7.718086099881295E-5 0.0 0.0 0.002489082767211718 0.0 30-31 7.718086099881295E-5 0.0 0.0 0.007602314808383076 0.0 32-33 7.718086099881295E-5 0.0 0.0 0.017674417168728168 0.0 34-35 7.718086099881295E-5 0.0 0.0 0.03249314248050025 0.0 36-37 7.718086099881295E-5 0.0 0.0 0.058888996942094284 0.0 38-39 7.718086099881295E-5 0.0 0.0 0.09749872265675047 0.0 40-41 7.718086099881295E-5 0.0 0.0 0.14218644117506318 0.0 42-43 7.718086099881295E-5 0.0 0.0 0.18625671280538536 0.0 44-45 7.718086099881295E-5 0.0 0.0 0.24379504468000046 0.0 46-47 1.1577129149821944E-4 0.0 0.0 0.3121001066639499 0.0 48-49 1.1577129149821944E-4 0.0 0.0 0.38837409254602684 0.0 50-51 1.1577129149821944E-4 0.0 0.0 0.4745079334207021 0.0 52-53 1.1577129149821944E-4 0.0 0.0 0.5632273331388375 0.0 54-55 1.1577129149821944E-4 0.0 0.0 0.6611505505310815 0.0 56-57 1.1577129149821944E-4 0.0 0.0 0.7794688104422618 0.0 58-59 1.1577129149821944E-4 0.0 0.0 0.9141301176699407 0.0 60-61 1.1577129149821944E-4 0.0 0.0 1.0648064535548734 0.0 62-63 1.1577129149821944E-4 0.0 0.0 1.2184349573730104 0.0 64-65 1.1577129149821944E-4 0.0 0.0 1.3805726511162666 0.0 66-67 1.1577129149821944E-4 0.0 0.0 1.5741615457165394 0.0 68-69 1.1577129149821944E-4 0.0 0.0 1.7907889273249578 0.0 70-71 1.1577129149821944E-4 0.0 0.0 2.037516844722913 0.0 72-73 1.1577129149821944E-4 0.0 0.0 2.296130614714685 0.0 74-75 1.1577129149821944E-4 0.0 0.0 2.5629448511875816 0.0 76-77 1.1577129149821944E-4 0.0 0.0 2.8563864847050686 0.0 78-79 1.1577129149821944E-4 0.0 0.0 3.184598096102521 0.0 80-81 1.1577129149821944E-4 0.0 0.0 3.5541979442105864 0.0 82-83 1.1577129149821944E-4 0.0 0.0 3.930763365023795 0.0 84-85 1.1577129149821944E-4 0.0 0.0 4.325736421185221 0.0 86-87 1.1577129149821944E-4 0.0 0.0 4.739676673937104 0.0 88-89 1.1577129149821944E-4 0.0 0.0 5.197764379180308 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6570 0.0 42.180176 1 GTATCAA 11100 0.0 39.15916 1 ATCAACG 13790 0.0 31.289135 3 TATCAAC 13970 0.0 31.021744 2 TCAACGC 14095 0.0 30.61207 4 CAACGCA 14160 0.0 30.404581 5 AACGCAG 14520 0.0 29.668486 6 ACGCAGA 16535 0.0 26.053003 7 CGCAGAG 16690 0.0 25.669071 8 GTACATG 12620 0.0 25.456003 1 TACATGG 12830 0.0 24.502098 2 GCAGAGT 17785 0.0 23.554361 9 ACATGGG 13110 0.0 23.038445 3 GAGTACT 8940 0.0 22.312822 12-13 GTACTTT 9510 0.0 20.75527 14-15 AGTACTT 9315 0.0 20.397243 12-13 GTCTACG 210 8.699044E-7 20.33687 1 CATGGGG 6225 0.0 19.727716 4 GTATAGG 675 0.0 19.68408 1 AGAGTAC 16850 0.0 19.473993 10-11 >>END_MODULE