FastQCFastQC Report
Fri 27 May 2016
SRR939353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2591316
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95780.36961914332331525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80240.3096496143272376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53390.2060343084363312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69900.046.087951
GTATCAA112250.037.0934831
TATCAAC146500.028.2777042
ATCAACG146850.028.1779943
TCAACGC148000.027.9911064
CAACGCA150350.027.5220385
AACGCAG155400.026.6887326
GTACATG124150.023.9557171
ACGCAGA175800.023.5107677
TACATGG125700.023.3680732
CGCAGAG178050.023.0804068
ACATGGG125550.022.375493
GTATAGG6050.022.0231061
GAGTACT98800.021.59063512-13
GCAGAGT189600.021.4996179
ATGGGCG2951.3460522E-1020.9116765
AGTACTT103850.020.33509312-13
GTACTTT105100.020.20612314-15
CATGGGG61300.019.5077694
TGGTATC38300.019.3282852
Produced by FastQC (version 0.11.5)