##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939353_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2591316 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27552911339258 33.0 31.0 34.0 31.0 34.0 2 32.48522874091774 34.0 31.0 34.0 31.0 34.0 3 32.56837761199329 34.0 31.0 34.0 31.0 34.0 4 36.10815199690042 37.0 35.0 37.0 35.0 37.0 5 36.04830595728194 37.0 35.0 37.0 35.0 37.0 6 36.07953101821623 37.0 35.0 37.0 35.0 37.0 7 36.02293660827163 37.0 35.0 37.0 35.0 37.0 8 35.99604949762978 37.0 35.0 37.0 35.0 37.0 9 37.69329869456292 39.0 37.0 39.0 35.0 39.0 10-11 37.6126981425654 39.0 37.0 39.0 35.0 39.0 12-13 37.5331925940333 39.0 37.0 39.0 35.0 39.0 14-15 38.81283949931232 40.0 38.0 41.0 35.0 41.0 16-17 38.81212036663996 40.0 38.0 41.0 34.5 41.0 18-19 38.92335554598513 40.0 38.0 41.0 35.0 41.0 20-21 38.91046557038972 40.0 38.0 41.0 35.0 41.0 22-23 38.82839337232511 40.0 38.0 41.0 34.5 41.0 24-25 38.76269490096924 40.0 38.0 41.0 34.0 41.0 26-27 38.60640577992032 40.0 38.0 41.0 34.0 41.0 28-29 38.43566126246278 40.0 38.0 41.0 34.0 41.0 30-31 38.230669281554235 40.0 38.0 41.0 34.0 41.0 32-33 38.17106983478665 40.0 38.0 41.0 33.5 41.0 34-35 38.077844616403404 40.0 38.0 41.0 33.0 41.0 36-37 37.92069010495054 40.0 37.0 41.0 33.0 41.0 38-39 37.785717372948724 40.0 37.0 41.0 33.0 41.0 40-41 37.591862204378 40.0 37.0 41.0 33.0 41.0 42-43 37.395987598579254 40.0 37.0 41.0 32.0 41.0 44-45 37.12737138195419 40.0 36.5 41.0 31.0 41.0 46-47 36.79720960315145 40.0 36.0 41.0 31.0 41.0 48-49 36.4678001447913 39.5 36.0 41.0 30.0 41.0 50-51 36.213894214368295 39.0 35.5 41.0 30.0 41.0 52-53 35.94545377715416 39.0 35.0 40.5 29.0 41.0 54-55 35.690327424366615 39.0 35.0 40.0 28.0 41.0 56-57 35.38443728977863 38.0 35.0 40.0 28.0 41.0 58-59 35.058252447791006 38.0 34.0 40.0 27.0 41.0 60-61 34.72477285672608 38.0 34.0 40.0 26.5 41.0 62-63 34.363416117524835 37.0 34.0 40.0 26.0 41.0 64-65 34.04454416211685 37.0 33.5 39.5 26.0 41.0 66-67 33.6315295008405 36.0 33.0 39.0 25.0 40.5 68-69 33.15828424630574 36.0 32.5 39.0 23.5 40.0 70-71 32.67706987492069 35.0 32.0 38.0 22.5 40.0 72-73 32.180244130781425 35.0 31.5 37.5 21.5 39.5 74-75 31.644782033530454 35.0 31.0 37.0 20.0 39.0 76-77 29.69597629158312 33.0 28.5 35.0 17.0 37.5 78-79 30.667405866362884 34.0 30.5 36.0 18.0 38.0 80-81 30.672659760523224 34.0 31.0 35.5 18.0 37.0 82-83 30.31105063990652 34.0 30.0 35.0 14.5 37.0 84-85 29.85006016248115 34.0 30.0 35.0 8.5 36.0 86-87 29.23713491523226 34.0 29.0 35.0 4.5 36.0 88-89 28.641538314894824 34.0 29.0 35.0 2.0 35.5 90-91 28.13659237236987 33.0 27.5 35.0 2.0 35.0 92-93 27.74588664601307 33.0 27.0 35.0 2.0 35.0 94-95 27.199411611706175 33.0 26.5 35.0 2.0 35.0 96-97 26.435374149659864 32.5 25.0 34.0 2.0 35.0 98-99 25.389349079772593 32.0 22.0 34.0 2.0 35.0 100-101 21.059994226871595 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29117352753621617 1101 2 0.25402448135424294 1101 3 0.27804087679447065 1101 4 0.12704031034878938 1101 5 0.22943542954151752 1101 6 0.22613469928763408 1101 7 0.21568284788737913 1101 8 0.20620431326207012 1101 9 0.30522043017635525 1101 10-11 0.31649851150091024 1101 12-13 0.3751054863179206 1101 14-15 0.4488600673690968 1101 16-17 0.40009762590027265 1101 18-19 0.4864597335290597 1101 20-21 0.48755777837804004 1101 22-23 0.3723859072370388 1101 24-25 0.39718712152157565 1101 26-27 0.44652745989157694 1101 28-29 0.47692985754088824 1101 30-31 0.4806960318221556 1101 32-33 0.5769032232950124 1101 34-35 0.7210377491307511 1101 36-37 0.518032317660257 1101 38-39 0.7426155452664887 1101 40-41 0.7064596009519732 1101 42-43 0.8044306843202094 1101 44-45 0.8146645153728613 1101 46-47 0.8273014374570948 1101 48-49 0.6807866513668728 1101 50-51 0.6697353373650898 1101 52-53 0.6287924414829149 1101 54-55 0.5465814964743672 1101 56-57 0.6542641347422418 1101 58-59 0.5658017990203774 1101 60-61 0.5212546640916287 1101 62-63 0.6951866024278033 1101 64-65 0.628691574513617 1101 66-67 0.5259384283625437 1101 68-69 0.6207792452641598 1101 70-71 0.5348440757847968 1101 72-73 0.6285689231751306 1101 74-75 0.541610383652408 1101 76-77 0.5745004271905074 1101 78-79 0.8347358497576174 1101 80-81 0.7984544393540638 1101 82-83 0.8932987015536149 1101 84-85 1.012541166601121 1101 86-87 0.724376732994795 1101 88-89 0.7834150562009476 1101 90-91 1.1742917554440986 1101 92-93 1.134483423292945 1101 94-95 1.058994123477916 1101 96-97 0.8513190207079262 1101 98-99 0.5891773123734687 1101 100-101 0.3527893142201002 1102 1 0.15636533320852664 1102 2 0.14041151437638177 1102 3 0.20790533706909287 1102 4 0.1714970453015212 1102 5 0.20735307330359376 1102 6 0.19247689653554545 1102 7 0.16736308327519112 1102 8 0.14453816279675635 1102 9 0.27044283921429724 1102 10-11 0.2206659155980759 1102 12-13 0.17186374185233433 1102 14-15 0.2656862033807812 1102 16-17 0.29570493678622256 1102 18-19 0.2774263942962847 1102 20-21 0.26455013122377835 1102 22-23 0.28645386883165713 1102 24-25 0.2860972729897995 1102 26-27 0.27499412065879625 1102 28-29 0.22365585049105619 1102 30-31 0.17162121212391668 1102 32-33 0.38727647374116003 1102 34-35 0.4317992567336617 1102 36-37 0.3936491782711471 1102 38-39 0.4692563281227109 1102 40-41 0.5720818816770148 1102 42-43 0.5155428401245246 1102 44-45 0.5328506575789618 1102 46-47 0.411399854931247 1102 48-49 0.4315699869124998 1102 50-51 0.5016955751126844 1102 52-53 0.558426639084793 1102 54-55 0.3906538457804487 1102 56-57 0.44253991162526773 1102 58-59 0.4933140403666272 1102 60-61 0.5384467499875356 1102 62-63 0.4233370898693707 1102 64-65 0.33438564409755855 1102 66-67 0.40064474666704797 1102 68-69 0.4571382559274042 1102 70-71 0.2659002237428467 1102 72-73 0.36358876005550655 1102 74-75 0.48335429616365033 1102 76-77 0.523624728475113 1102 78-79 0.5720717714719967 1102 80-81 0.5645477538091761 1102 82-83 0.6207178422996016 1102 84-85 0.6217599696039393 1102 86-87 0.34721698342619334 1102 88-89 0.5520508466527012 1102 90-91 0.6926307301207366 1102 92-93 0.570403109737498 1102 94-95 0.6857420181138458 1102 96-97 0.35052628292346455 1102 98-99 0.25585448742472394 1102 100-101 0.1830731880899883 1103 1 0.16295837466424246 1103 2 0.1369226237386698 1103 3 0.20715771764672297 1103 4 0.1457282214686373 1103 5 0.15891301839072725 1103 6 0.18795620365411025 1103 7 0.2550542508731013 1103 8 0.21418700027041382 1103 9 0.2782786340080676 1103 10-11 0.18200994732162457 1103 12-13 0.20400919099235182 1103 14-15 0.27456090498881736 1103 16-17 0.28574924744613384 1103 18-19 0.17785693127065372 1103 20-21 0.23448960028282784 1103 22-23 0.21407250422947044 1103 24-25 0.28804392531615974 1103 26-27 0.23728852531764488 1103 28-29 0.2677298735579541 1103 30-31 0.3599863302128057 1103 32-33 0.34845327480444865 1103 34-35 0.35499065167783783 1103 36-37 0.3316011382582076 1103 38-39 0.3687069808687937 1103 40-41 0.3914208505742991 1103 42-43 0.3432061796661614 1103 44-45 0.34048184074134724 1103 46-47 0.3334780183232624 1103 48-49 0.13073131968621254 1103 50-51 0.12234429891321241 1103 52-53 0.07042188667360705 1103 54-55 0.04318624262208459 1103 56-57 0.10359232805365792 1103 58-59 0.07747314339820122 1103 60-61 0.14325446751855253 1103 62-63 0.037688431649492316 1103 64-65 0.04621442613370874 1103 66-67 -0.0758123460989708 1103 68-69 0.003470985918745839 1103 70-71 8.822546444520185E-4 1103 72-73 0.07035580883876236 1103 74-75 -0.022349580964441174 1103 76-77 0.17337268017277552 1103 78-79 0.08420473842809173 1103 80-81 0.1226642337895889 1103 82-83 0.1789031819636513 1103 84-85 0.11616320753928733 1103 86-87 0.13611997215506477 1103 88-89 0.2109478456821101 1103 90-91 0.28595254311969853 1103 92-93 0.03991411854279292 1103 94-95 -0.046165919768160535 1103 96-97 -0.2893353856231826 1103 98-99 -0.6908661521177883 1103 100-101 -0.5202437719407911 1104 1 0.07363315948988003 1104 2 0.0641552412507238 1104 3 0.05592588110779673 1104 4 0.03161100051620025 1104 5 0.04964504628221533 1104 6 0.07187381664938641 1104 7 0.0612233018422117 1104 8 0.09110804204893697 1104 9 0.07282505062185862 1104 10-11 0.11751670620537169 1104 12-13 0.18390054801096767 1104 14-15 0.12633199497686576 1104 16-17 0.17444713747465102 1104 18-19 0.08295967804649251 1104 20-21 0.15123638036037335 1104 22-23 0.19460972035741264 1104 24-25 0.2045197699732526 1104 26-27 0.27480388480933726 1104 28-29 0.2159391177613159 1104 30-31 0.1795659770912792 1104 32-33 0.13083836953634176 1104 34-35 0.2860107058507708 1104 36-37 0.31689564081582233 1104 38-39 0.28699784525181826 1104 40-41 0.371499315735754 1104 42-43 0.39098224552822813 1104 44-45 0.3301484486935493 1104 46-47 0.28807794291407873 1104 48-49 0.32319591946224335 1104 50-51 0.3931228527122883 1104 52-53 0.37758059470072425 1104 54-55 0.4054161118727535 1104 56-57 0.27265267025287443 1104 58-59 0.23638189616857375 1104 60-61 0.3036616770888756 1104 62-63 0.32505841978633754 1104 64-65 0.2673083402733525 1104 66-67 0.2929298768872428 1104 68-69 0.24463728862647116 1104 70-71 0.21684731883068054 1104 72-73 0.1732769595573842 1104 74-75 0.10586636234709701 1104 76-77 0.18267945334606495 1104 78-79 0.34761621859144753 1104 80-81 0.31395984979982217 1104 82-83 0.22662654628831902 1104 84-85 0.009877824116149014 1104 86-87 0.08945116913077555 1104 88-89 0.25899467011383237 1104 90-91 0.3373165579440389 1104 92-93 0.3300985102574039 1104 94-95 0.2563046504907689 1104 96-97 0.34037105620245356 1104 98-99 0.2971290389553509 1104 100-101 0.46241017137215934 1105 1 0.12570590290288663 1105 2 0.09566400504561301 1105 3 0.06361559520259874 1105 4 0.047502506387054666 1105 5 0.031699074495563195 1105 6 0.0985690502405916 1105 7 0.08740013158381998 1105 8 -0.050066901810595255 1105 9 0.05222396888126468 1105 10-11 0.05403702637103436 1105 12-13 0.09527751207251356 1105 14-15 0.12920740946913156 1105 16-17 0.0522816455945474 1105 18-19 0.09472703508619418 1105 20-21 0.07419912333225653 1105 22-23 -0.045218313869547444 1105 24-25 0.1526752815533925 1105 26-27 0.1235319488205775 1105 28-29 0.13648004960933235 1105 30-31 0.16732039591988013 1105 32-33 0.13823306191529383 1105 34-35 0.23146126752903484 1105 36-37 0.13590242554589338 1105 38-39 0.2561350345386373 1105 40-41 0.3122642383974892 1105 42-43 0.3023536528202797 1105 44-45 0.247183999365987 1105 46-47 0.26525928270078225 1105 48-49 0.2672415588344066 1105 50-51 0.2233281522631998 1105 52-53 0.32199930702796564 1105 54-55 0.3757026975576281 1105 56-57 0.2898617289156036 1105 58-59 0.20928554930852528 1105 60-61 0.22276631225532384 1105 62-63 0.29919593222067675 1105 64-65 0.23902746113428464 1105 66-67 0.2747129912154662 1105 68-69 0.2545898902375612 1105 70-71 0.20244152214552713 1105 72-73 0.23860302566207992 1105 74-75 0.2532952365524359 1105 76-77 0.3337891316655366 1105 78-79 0.3222379393707868 1105 80-81 0.35376179285678333 1105 82-83 0.4908670077087791 1105 84-85 0.5072132249096875 1105 86-87 0.4550123977205409 1105 88-89 0.4625111819261569 1105 90-91 0.6639285938725727 1105 92-93 0.6751779139288381 1105 94-95 0.6409884326913904 1105 96-97 0.6038115992817801 1105 98-99 0.5574703579648705 1105 100-101 0.5399165052344976 1106 1 0.05359560355787352 1106 2 0.03873759423637324 1106 3 0.07367273558480036 1106 4 0.058746509248841505 1106 5 0.050365492929550726 1106 6 0.038227824045513614 1106 7 0.02965629833153116 1106 8 -0.015579044121608376 1106 9 0.06599732695383409 1106 10-11 0.10884077306833717 1106 12-13 0.07600885466382579 1106 14-15 0.16402608244892747 1106 16-17 0.04690868488819433 1106 18-19 0.06033242626033086 1106 20-21 0.1262044344772093 1106 22-23 0.23754270254629262 1106 24-25 0.23612162054968877 1106 26-27 0.1595211616886658 1106 28-29 0.14666397289121136 1106 30-31 0.13223708686842173 1106 32-33 0.10299153685517837 1106 34-35 0.1482889379736534 1106 36-37 0.1272631688734691 1106 38-39 0.15397015794308544 1106 40-41 0.1616400124074957 1106 42-43 0.2590811704304983 1106 44-45 0.30381918928903673 1106 46-47 0.22616013136240554 1106 48-49 0.20956350077672425 1106 50-51 0.22840488217909893 1106 52-53 0.2198446118507391 1106 54-55 0.04548002993875144 1106 56-57 0.11091691818251803 1106 58-59 0.1410010391082821 1106 60-61 0.28193707936701884 1106 62-63 0.24429829022100336 1106 64-65 0.1124027774652987 1106 66-67 0.1734599171947835 1106 68-69 0.28720380131951373 1106 70-71 0.2395083492584149 1106 72-73 0.2569450931978494 1106 74-75 0.1333280692024026 1106 76-77 0.09002973506487066 1106 78-79 0.32355505936106255 1106 80-81 0.3773263623613694 1106 82-83 0.5148252587221123 1106 84-85 0.45112920525987477 1106 86-87 0.4661533743254189 1106 88-89 0.6535347102142559 1106 90-91 0.5181075731869988 1106 92-93 0.558024373453744 1106 94-95 0.609289071551153 1106 96-97 0.6671481825884875 1106 98-99 0.5720184734893827 1106 100-101 0.4340905198954168 1107 1 -0.031084414035028374 1107 2 -0.013995469927515103 1107 3 0.008473209801913129 1107 4 0.026505072474492408 1107 5 0.05646411365174231 1107 6 0.015400114304128465 1107 7 0.10197379855600985 1107 8 0.09023665952617677 1107 9 0.11104673471510296 1107 10-11 0.03185635861841263 1107 12-13 0.0371060828678722 1107 14-15 0.1076392941740778 1107 16-17 0.09253138399927252 1107 18-19 0.07134382055907196 1107 20-21 0.1337100311826518 1107 22-23 0.12430563812672801 1107 24-25 0.10789436463225144 1107 26-27 0.17504089055092464 1107 28-29 0.13690881258422394 1107 30-31 0.14610276289412383 1107 32-33 0.06170611324812114 1107 34-35 0.07379347849592932 1107 36-37 0.03996795654869345 1107 38-39 0.02154738943438872 1107 40-41 0.060541825420465045 1107 42-43 0.11756472578043287 1107 44-45 -0.0024606109797318254 1107 46-47 0.023036933836372953 1107 48-49 0.15798021525571926 1107 50-51 0.21106879221785846 1107 52-53 0.1859377842505765 1107 54-55 0.17841323624602268 1107 56-57 0.16342200505891213 1107 58-59 0.13788491831379446 1107 60-61 0.21294853454086393 1107 62-63 0.15523929767363853 1107 64-65 0.05233898034986595 1107 66-67 0.1702935744344316 1107 68-69 0.23730782166398257 1107 70-71 0.23606700318797635 1107 72-73 0.34482242376339656 1107 74-75 0.1860591549252142 1107 76-77 0.11311875121655746 1107 78-79 0.21858011707827885 1107 80-81 0.2658364136029938 1107 82-83 0.3293493751579355 1107 84-85 0.23725987672755977 1107 86-87 0.34662638915003186 1107 88-89 0.5160653950744667 1107 90-91 0.4553314094451295 1107 92-93 0.3112270890924087 1107 94-95 0.5605451533551253 1107 96-97 0.5678881956462014 1107 98-99 0.35339342916469363 1107 100-101 0.22861495592556835 1108 1 0.09094709962828063 1108 2 0.08795471152057388 1108 3 0.09667636704272553 1108 4 0.0650227348415271 1108 5 -0.0054618942993656106 1108 6 0.05064099013077339 1108 7 0.07476143159468762 1108 8 0.08640223770012767 1108 9 0.14445726854404484 1108 10-11 0.13931834704274593 1108 12-13 0.12422826183105684 1108 14-15 0.1023781073229344 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-0.8445506418808542 2316 90-91 -0.9240285946916593 2316 92-93 -0.7017331287785247 2316 94-95 -0.5968881409085292 2316 96-97 -0.3549201675639502 2316 98-99 -0.19569475340562903 2316 100-101 0.40172237456870263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 28.0 5 62.0 6 133.0 7 330.0 8 737.0 9 1449.0 10 2408.0 11 3645.0 12 5584.0 13 7556.0 14 9612.0 15 12962.0 16 17398.0 17 23811.0 18 22689.0 19 15511.0 20 14465.0 21 15399.0 22 16194.0 23 18178.0 24 20642.0 25 24539.0 26 29284.0 27 35866.0 28 43475.0 29 52751.0 30 64068.0 31 78303.0 32 97598.0 33 124860.0 34 171161.0 35 244354.0 36 379413.0 37 590553.0 38 422487.0 39 23804.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.299995472099425 19.69191700087346 15.157630925019127 22.850456602007988 2 15.532300962136613 20.720166895893826 39.99554666432037 23.751985477649196 3 19.071313571945684 24.858604662650173 30.194889392108102 25.87519237329604 4 13.173229355277396 17.132298801072505 36.63381849222557 33.06065335142453 5 13.728391288441857 37.73526655953963 33.86005411921973 14.676288032798778 6 31.89340860010898 36.152673004758974 17.310046323952772 14.64387207117928 7 28.456313317248842 33.18194307448416 20.37891171898757 17.982831889279424 8 26.368956931535948 35.75364795339511 19.357230071515787 18.52016504355316 9 26.60200454132186 16.985269260869767 20.24963377681456 36.16309242099381 10-11 25.07351477010137 27.026344915093336 26.864631716085576 21.035508598719723 12-13 25.887251882827105 24.253410236343232 27.73534759944368 22.123990281385982 14-15 22.676426350374186 25.48313618116196 26.176965178305256 25.6634722901586 16-17 22.02286393264504 28.92522690949071 26.802497523913317 22.249411633950935 18-19 21.955648001111406 27.150848121513594 29.494735298487445 21.39876857888755 20-21 22.764417770738884 26.294014315506097 29.47064348771049 21.470924426044526 22-23 22.506749466294345 26.30165522074498 29.32257200588427 21.869023307076404 24-25 22.14166469855471 26.449977540369453 29.496421895284097 21.911935865791747 26-27 21.959337625749402 26.76409984199139 29.34072649228799 21.935836039971218 28-29 21.845714687054762 26.82266462291747 29.51832196459251 21.813298725435263 30-31 22.07793260258494 26.54896199460043 29.428676394542386 21.944429008272245 32-33 21.85493779994412 26.637449851735568 29.286875857672317 22.220736490647994 34-35 22.36550463006646 26.698033531440863 29.129872442831594 21.80658939566108 36-37 22.37231442419873 26.49467737970315 29.073706220999405 22.059301975098712 38-39 22.09511684443517 26.744388651862067 29.206661123641553 21.9538333800612 40-41 22.257006170999254 26.559763911666007 29.19831423914518 21.984915678189555 42-43 22.27677208700313 26.577610003284054 28.87069816837745 22.274919741335363 44-45 22.212619508918657 26.83629702137726 28.721522536243167 22.229560933460917 46-47 22.24740469006205 27.071453076418134 28.27555494153727 22.405587291982545 48-49 22.410887495595844 27.078655024132647 28.139472689994182 22.370984790277323 50-51 22.446166821446482 27.2405077652644 27.911595481136896 22.40172993215222 52-53 22.67596671032534 27.2614202678641 27.761089547619473 22.301523474191086 54-55 22.218131636589288 27.11516079088772 27.968086485785598 22.698621086737393 56-57 22.30426618983917 27.47347994351084 27.82477168255931 22.397482184090688 58-59 22.40770234437801 27.04964721836417 27.97518044289599 22.567469994361822 60-61 22.46643240857924 27.04247267251611 28.15563916433499 22.335455754569665 62-63 22.422238507553576 27.098675601216225 28.085346713546812 22.393739177683383 64-65 22.17031809320052 26.958483643060127 28.08634686005103 22.784851403688318 66-67 22.142043707002777 27.2709345614423 27.86945630785463 22.717565423700297 68-69 22.175996542239382 27.08701998728424 28.045043506616924 22.691939963859458 70-71 22.075192682019484 27.219567200603862 27.831051867082206 22.874188250294445 72-73 22.206805345237708 27.11315408850175 27.755395328088124 22.92464523817242 74-75 22.141649675618428 27.285948005945244 27.55492335842548 23.017478960010852 76-77 22.190192809071103 27.061926519870454 27.760544315598118 22.987336355460325 78-79 22.14344399417901 27.158318704316077 27.692584950243333 23.00565235126158 80-81 22.034110879654108 27.43401610148365 27.605415630834397 22.92645738802785 82-83 22.168542933397546 27.302903235267333 27.49265238203291 23.035901449302205 84-85 22.136262038284794 27.186282954298125 27.393745108662937 23.283709898754147 86-87 22.16210285742542 27.01241697101934 27.6475592164583 23.17792095509694 88-89 22.09885105869515 26.87398522709511 27.659579001069318 23.367584713140417 90-91 22.048026438630895 27.00915890364044 27.68591355395839 23.25690110377027 92-93 22.091998814501977 26.83015116643435 27.614270123751794 23.46357989531188 94-95 21.920850255237106 26.74017757772499 27.600628406570255 23.738343760467654 96-97 22.054141602182057 26.63465204552436 27.524393011118676 23.78681334117491 98-99 21.80137181368217 26.631623956031575 27.41790458431514 24.149099645971116 100-101 21.919676425772096 26.10146657680209 28.170712341438573 23.808144655987242 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 538.0 1 382.0 2 290.5 3 393.0 4 371.0 5 369.0 6 661.5 7 1359.5 8 2257.5 9 2717.0 10 2555.0 11 2369.0 12 2572.0 13 2938.5 14 3241.5 15 3477.0 16 3696.5 17 3896.0 18 4216.5 19 4501.5 20 4851.5 21 5577.5 22 6624.5 23 7869.5 24 9339.0 25 11514.0 26 14290.0 27 17600.0 28 20968.0 29 24594.0 30 29134.5 31 34458.0 32 39983.5 33 44471.0 34 48503.5 35 53266.0 36 59246.0 37 64967.5 38 69210.0 39 72130.5 40 74307.0 41 79201.0 42 84405.5 43 88329.0 44 92904.0 45 97511.5 46 98762.0 47 99122.5 48 105498.5 49 106706.5 50 101699.5 51 98186.0 52 94077.0 53 91782.0 54 87869.0 55 79165.5 56 70200.5 57 63035.0 58 56045.5 59 48484.5 60 40992.0 61 33700.5 62 27004.0 63 20953.5 64 16290.5 65 12735.0 66 9805.0 67 8135.5 68 6252.5 69 4239.0 70 3111.5 71 2311.0 72 1778.5 73 1498.5 74 1210.0 75 1009.5 76 683.0 77 390.5 78 277.0 79 173.5 80 113.0 81 77.5 82 55.5 83 36.0 84 19.5 85 14.5 86 9.5 87 6.0 88 5.0 89 2.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28313798857414535 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001678683726724182 16-17 0.0011384176997324912 18-19 3.8590430499406476E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.788564574910972E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0017944550182224012 36-37 0.007911038252378329 38-39 0.0027592157807075635 40-41 0.003183710516201035 42-43 2.701330134958454E-4 44-45 0.0013313698522295235 46-47 2.701330134958454E-4 48-49 5.016755964922842E-4 50-51 1.9295215249703238E-5 52-53 7.718086099881295E-5 54-55 0.0 56-57 8.682846862366458E-4 58-59 0.002045292816468543 60-61 5.59561242241394E-4 62-63 0.001041941623483975 64-65 0.0 66-67 5.788564574910972E-4 68-69 0.001678683726724182 70-71 0.0 72-73 0.0 74-75 1.9295215249703238E-5 76-77 0.259038264727266 78-79 1.9295215249703242E-4 80-81 7.718086099881295E-5 82-83 0.0 84-85 0.0 86-87 0.1065867690393607 88-89 0.376854849041954 90-91 0.2785650225599657 92-93 0.0 94-95 0.0 96-97 0.0 98-99 7.718086099881295E-5 100-101 3.6660908974436157E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2591316.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.3225694883411 #Duplication Level Percentage of deduplicated Percentage of total 1 69.91632382037653 33.78536416179504 2 15.424768896316287 14.907289336676927 3 5.9462986376308615 8.62021287346036 4 2.9462833677385394 5.694879310795569 5 1.6316956248374366 3.94238626075146 6 0.9599727185029182 2.7833009038061385 7 0.6334482280663503 2.1426892212601927 8 0.44676274658493853 1.7270979093322254 9 0.32059096759106487 1.3942601377878372 >10 1.596562332365283 13.778761038373617 >50 0.11333595423313719 3.8201611179903083 >100 0.059881956499818915 5.176014370161655 >500 0.0033556758584905194 1.0357835992073188 >1k 4.793822654954516E-4 0.30608663987145873 >5k 2.396911327477258E-4 0.8857131187299614 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9578 0.36961914332331525 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8024 0.3096496143272376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5339 0.2060343084363312 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.8590430499406476E-5 0.0 22-23 0.0 0.0 0.0 1.1577129149821944E-4 0.0 24-25 0.0 0.0 0.0 2.894282287455486E-4 0.0 26-27 0.0 0.0 0.0 9.068751167360522E-4 0.0 28-29 0.0 0.0 0.0 0.0025662636282105305 0.0 30-31 0.0 0.0 0.0 0.007891743037128626 0.0 32-33 0.0 0.0 0.0 0.01817609276522045 0.0 34-35 0.0 0.0 0.0 0.033496493673484826 0.0 36-37 0.0 0.0 0.0 0.06018177636382441 0.0 38-39 0.0 0.0 0.0 0.09896515901572792 0.0 40-41 0.0 0.0 0.0 0.14396160097803587 0.0 42-43 0.0 0.0 0.0 0.18855284342010004 0.0 44-45 0.0 0.0 0.0 0.24630342266246186 0.0 46-47 0.0 0.0 0.0 0.3150522745971545 0.0 48-49 0.0 0.0 0.0 0.39157709827747755 0.0 50-51 0.0 0.0 0.0 0.4783090908248936 0.0 52-53 0.0 0.0 0.0 0.5677231182920184 0.0 54-55 0.0 0.0 0.0 0.6662830777875026 0.0 56-57 0.0 0.0 0.0 0.7851030132951751 0.0 58-59 0.0 0.0 0.0 0.9204782434870931 0.0 60-61 0.0 0.0 0.0 1.072505244439505 0.0 62-63 0.0 0.0 0.0 1.2265968334236348 0.0 64-65 0.0 0.0 0.0 1.3896221070683776 0.0 66-67 0.0 0.0 0.0 1.5837705629108916 3.8590430499406476E-5 68-69 0.0 0.0 0.0 1.8006873727480555 3.8590430499406476E-5 70-71 0.0 0.0 0.0 2.0481292131102498 3.8590430499406476E-5 72-73 0.0 0.0 0.0 2.307418315635762 3.8590430499406476E-5 74-75 0.0 0.0 0.0 2.5759691214811316 3.8590430499406476E-5 76-77 0.0 0.0 0.0 2.8702404492543554 3.8590430499406476E-5 78-79 0.0 0.0 0.0 3.1998413161497865 3.8590430499406476E-5 80-81 0.0 0.0 0.0 3.5711584384150754 3.8590430499406476E-5 82-83 0.0 0.0 0.0 3.9481290587485276 3.8590430499406476E-5 84-85 0.0 0.0 0.0 4.343835333089443 3.8590430499406476E-5 86-87 0.0 0.0 0.0 4.759319203061302 3.8590430499406476E-5 88-89 0.0 0.0 0.0 5.217445498735006 3.8590430499406476E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6990 0.0 46.08795 1 GTATCAA 11225 0.0 37.093483 1 TATCAAC 14650 0.0 28.277704 2 ATCAACG 14685 0.0 28.177994 3 TCAACGC 14800 0.0 27.991106 4 CAACGCA 15035 0.0 27.522038 5 AACGCAG 15540 0.0 26.688732 6 GTACATG 12415 0.0 23.955717 1 ACGCAGA 17580 0.0 23.510767 7 TACATGG 12570 0.0 23.368073 2 CGCAGAG 17805 0.0 23.080406 8 ACATGGG 12555 0.0 22.37549 3 GTATAGG 605 0.0 22.023106 1 GAGTACT 9880 0.0 21.590635 12-13 GCAGAGT 18960 0.0 21.499617 9 ATGGGCG 295 1.3460522E-10 20.911676 5 AGTACTT 10385 0.0 20.335093 12-13 GTACTTT 10510 0.0 20.206123 14-15 CATGGGG 6130 0.0 19.507769 4 TGGTATC 3830 0.0 19.328285 2 >>END_MODULE