FastQCFastQC Report
Fri 27 May 2016
SRR939352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637678
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93360.35394767670655786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71270.2701997741953339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49330.1870205536839599No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66900.040.5437551
GTATCAA110450.038.5780871
ATCAACG138750.030.519793
TATCAAC139050.030.4880472
TCAACGC141350.029.9584084
CAACGCA141600.029.8379735
AACGCAG146150.028.988226
GTACATG126500.025.422141
ACGCAGA166500.025.359837
CGCAGAG169150.024.9345118
TACATGG127250.024.9305252
ACATGGG130550.023.1743133
GCAGAGT180450.023.1100259
GAGTACT88800.022.3339412-13
CATGGGG61850.021.3908274
AGTACTT93300.021.02817312-13
GTACTTT96050.020.37716714-15
AGAGTAC167000.019.26779710-11
CAGAGTA175400.019.16909210-11
CATGGGA71500.018.2385254
Produced by FastQC (version 0.11.5)