##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939352_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2637678 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.828600003487917 33.0 31.0 34.0 30.0 34.0 2 31.90090374943416 33.0 31.0 34.0 30.0 34.0 3 31.95186827201804 34.0 31.0 34.0 30.0 34.0 4 35.462570109012546 37.0 35.0 37.0 33.0 37.0 5 35.50715022834478 37.0 35.0 37.0 33.0 37.0 6 35.60268994168356 37.0 35.0 37.0 33.0 37.0 7 35.52071367316253 37.0 35.0 37.0 33.0 37.0 8 35.504408043741506 37.0 35.0 37.0 33.0 37.0 9 37.127640674866306 39.0 37.0 39.0 33.0 39.0 10-11 37.03564783116059 39.0 37.0 39.0 33.0 39.0 12-13 36.928079356160985 39.0 37.0 39.0 33.0 39.0 14-15 38.15167545090796 40.0 38.0 41.0 33.0 41.0 16-17 38.090320918626155 40.0 38.0 41.0 33.0 41.0 18-19 38.0379798064813 40.0 38.0 41.0 33.0 41.0 20-21 37.94575740480832 40.0 38.0 41.0 33.0 41.0 22-23 37.86519146764692 40.0 38.0 41.0 32.5 41.0 24-25 37.88463053488712 40.0 38.0 41.0 33.0 41.0 26-27 37.8050448159328 40.0 38.0 41.0 32.0 41.0 28-29 37.670672083552276 40.0 37.5 41.0 32.0 41.0 30-31 37.490838153861084 40.0 37.0 41.0 32.0 41.0 32-33 37.29177139893497 40.0 37.0 41.0 31.5 41.0 34-35 36.99191883922147 40.0 36.0 41.0 30.5 41.0 36-37 36.74804790425519 40.0 36.0 41.0 30.0 41.0 38-39 36.43414264364338 39.5 35.5 41.0 30.0 41.0 40-41 36.29775241708806 39.0 35.0 41.0 30.0 41.0 42-43 36.11936009626649 39.0 35.0 41.0 29.5 41.0 44-45 36.065869867360604 39.0 35.0 41.0 29.0 41.0 46-47 35.865963737802716 39.0 35.0 41.0 27.5 41.0 48-49 35.666980768691246 39.0 35.0 41.0 27.0 41.0 50-51 34.525373643029965 38.0 34.0 40.0 25.0 40.5 52-53 34.64208576634449 38.5 34.5 39.5 25.0 40.5 54-55 35.141877628732544 39.0 35.0 40.5 26.0 41.0 56-57 35.053510701457874 39.0 35.0 41.0 26.0 41.0 58-59 34.851164926120624 38.5 34.5 40.5 25.5 41.0 60-61 34.56841111765728 38.0 34.0 40.0 25.0 41.0 62-63 34.21196256707604 38.0 34.0 40.0 23.5 41.0 64-65 33.83808069066808 37.0 34.0 40.0 22.5 41.0 66-67 33.48867602489766 36.5 33.5 39.5 22.0 41.0 68-69 33.08002948047488 36.0 33.0 39.0 21.5 41.0 70-71 32.66761788209175 35.5 32.5 39.0 20.0 40.5 72-73 32.22831539710306 35.0 32.0 38.0 18.5 40.0 74-75 31.781193155495096 35.0 32.0 37.0 17.5 39.5 76-77 31.33494270339291 35.0 31.0 37.0 13.5 39.0 78-79 30.90787370558499 35.0 31.0 36.0 9.0 39.0 80-81 30.44773452256113 35.0 31.0 36.0 7.0 37.5 82-83 30.010467160889235 34.0 30.0 35.0 2.0 37.0 84-85 29.599969556556943 34.0 30.0 35.0 2.0 36.5 86-87 29.27300034348393 34.0 29.5 35.0 2.0 36.0 88-89 28.871617763805894 34.0 29.0 35.0 2.0 36.0 90-91 28.475465542041142 34.0 29.0 35.0 2.0 35.5 92-93 28.061952596184977 34.0 29.0 35.0 2.0 35.0 94-95 27.602730697226878 34.0 27.0 35.0 2.0 35.0 96-97 27.03331471847587 34.0 26.5 35.0 2.0 35.0 98-99 26.303199442843287 33.0 25.0 35.0 2.0 35.0 100-101 23.852877606743505 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3056158649996128 1101 2 0.08195284752139287 1101 3 0.38652677882762987 1101 4 0.4189366881455143 1101 5 0.3021002691900989 1101 6 0.3082260977333746 1101 7 0.24852639816180044 1101 8 0.21881207473767716 1101 9 0.3222390454530384 1101 10-11 0.4048400003949695 1101 12-13 0.3527315319464108 1101 14-15 0.37463089688528584 1101 16-17 0.4869400449186543 1101 18-19 0.5682168791804472 1101 20-21 0.5311109050068623 1101 22-23 0.4709281481412546 1101 24-25 0.5029357470387268 1101 26-27 0.4232843813448568 1101 28-29 0.4365646272812498 1101 30-31 0.40341951467519976 1101 32-33 0.5488240850464337 1101 34-35 0.4971586752264727 1101 36-37 0.5793396765257697 1101 38-39 0.6672398736205309 1101 40-41 0.7175612494280799 1101 42-43 0.4801974553857704 1101 44-45 0.6157885917623744 1101 46-47 0.4786788234446959 1101 48-49 0.5883616862964516 1101 50-51 0.5214880886423856 1101 52-53 0.6727578291199308 1101 54-55 0.6614477917949415 1101 56-57 0.6085678693204599 1101 58-59 0.6023703113538161 1101 60-61 0.6672381010280262 1101 62-63 0.6671222275027873 1101 64-65 0.5734937257529609 1101 66-67 0.5066203240388916 1101 68-69 0.5247868736064731 1101 70-71 0.4719428462092239 1101 72-73 0.38881904700118497 1101 74-75 0.49313803403086354 1101 76-77 0.2748141729605642 1101 78-79 0.210307267385911 1101 80-81 0.0310465142634051 1101 82-83 -0.08296497765800481 1101 84-85 -0.062417874545435126 1101 86-87 0.06585447231201513 1101 88-89 -0.21463951220248845 1101 90-91 -0.13855838944620658 1101 92-93 -0.042581868883889484 1101 94-95 -0.10652763229291651 1101 96-97 -0.25459377689369944 1101 98-99 -0.32353579246735364 1101 100-101 -0.4361424789459072 1102 1 0.30567868727257874 1102 2 0.23699504079517197 1102 3 0.3412993304354188 1102 4 0.2457863276233141 1102 5 0.26421173284317945 1102 6 0.20357583779740196 1102 7 0.2812128620795846 1102 8 0.360721765955617 1102 9 0.3283262766495909 1102 10-11 0.3296175636672274 1102 12-13 0.4427778192559586 1102 14-15 0.45595856444958116 1102 16-17 0.46114709018951316 1102 18-19 0.45840257546652197 1102 20-21 0.4512832074431614 1102 22-23 0.4173058970568633 1102 24-25 0.43588902914955696 1102 26-27 0.4139427446481747 1102 28-29 0.4345288150563178 1102 30-31 0.3792309102119944 1102 32-33 0.3834327164032203 1102 34-35 0.3062059648689157 1102 36-37 0.24441155155886207 1102 38-39 0.35952570437450504 1102 40-41 0.34174191287362987 1102 42-43 0.3930641980509222 1102 44-45 0.5576875184897219 1102 46-47 0.4174911648449182 1102 48-49 0.3330174696513737 1102 50-51 0.3551323275223197 1102 52-53 0.481096338820322 1102 54-55 0.45003346125802324 1102 56-57 0.45318571225771365 1102 58-59 0.23954768545339533 1102 60-61 0.23310443076025678 1102 62-63 0.28262838796781864 1102 64-65 0.40056624312614275 1102 66-67 0.3202765803255332 1102 68-69 0.22631093136060798 1102 70-71 0.2503768069417802 1102 72-73 0.3426536761909489 1102 74-75 0.12918346018116011 1102 76-77 0.22906791141520344 1102 78-79 0.18410473261307558 1102 80-81 0.09490985464365664 1102 82-83 0.1067172581090432 1102 84-85 -0.07185489168744752 1102 86-87 0.027970524108628325 1102 88-89 0.11946636450350567 1102 90-91 -0.05353858984219784 1102 92-93 0.1916715342833406 1102 94-95 0.15482069956397027 1102 96-97 -0.009515796100373564 1102 98-99 -0.2109344562587836 1102 100-101 -0.2727273648952462 1103 1 0.26419568338655 1103 2 0.3611655771232307 1103 3 0.4076536183943418 1103 4 0.3356595056090299 1103 5 0.35536435415881584 1103 6 0.36541068859915526 1103 7 0.3257773322750097 1103 8 0.4186551563401508 1103 9 0.5111730380586579 1103 10-11 0.40940635731490715 1103 12-13 0.40859444640765474 1103 14-15 0.5616518768329612 1103 16-17 0.5515512652151102 1103 18-19 0.5391562124805915 1103 20-21 0.5281262652004912 1103 22-23 0.5701828909262048 1103 24-25 0.6206602164224648 1103 26-27 0.5858454957319168 1103 28-29 0.6113052351402146 1103 30-31 0.5801862840142675 1103 32-33 0.6028607547304077 1103 34-35 0.46794822437706074 1103 36-37 0.575138533503555 1103 38-39 0.7072631799991456 1103 40-41 0.5286006129479901 1103 42-43 0.4704550876287925 1103 44-45 0.5899990741637495 1103 46-47 0.6520508651858279 1103 48-49 0.48038528155075966 1103 50-51 0.4260793540791852 1103 52-53 0.47735515954394003 1103 54-55 0.6464192442857595 1103 56-57 0.704620877308713 1103 58-59 0.6945104385011902 1103 60-61 0.7202731713783734 1103 62-63 0.593011238937919 1103 64-65 0.6511277532059765 1103 66-67 0.6460074903857134 1103 68-69 0.6007848444650818 1103 70-71 0.5641413444763188 1103 72-73 0.596500072846446 1103 74-75 0.6493059332104423 1103 76-77 0.7131036292081667 1103 78-79 0.5366555526071828 1103 80-81 0.427990900083536 1103 82-83 0.5488564677160888 1103 84-85 0.4571570469778372 1103 86-87 0.43207189754614816 1103 88-89 0.414709486794159 1103 90-91 0.5147359152853959 1103 92-93 0.5964638351769871 1103 94-95 0.5290585750146519 1103 96-97 0.5382760418368022 1103 98-99 0.3724710094333368 1103 100-101 0.2976139602825718 1104 1 0.11706231048930604 1104 2 0.15794481605411193 1104 3 0.17320278949346601 1104 4 0.2123927376291732 1104 5 0.23719883466064573 1104 6 0.14453245300162365 1104 7 0.23588845324628238 1104 8 0.18263401922902034 1104 9 0.2524028840367265 1104 10-11 0.14354173798811587 1104 12-13 0.1428584328967588 1104 14-15 0.2281957151238032 1104 16-17 0.1864646590775294 1104 18-19 0.21550390437017342 1104 20-21 0.2625178175662839 1104 22-23 0.24990067915575054 1104 24-25 0.2641160176193651 1104 26-27 0.1698763971789532 1104 28-29 0.22822629404791428 1104 30-31 0.29704896766752853 1104 32-33 0.290123648590054 1104 34-35 0.2735794258812305 1104 36-37 0.3280860678770168 1104 38-39 0.43414706454647956 1104 40-41 0.27140284701473405 1104 42-43 0.21179343393132655 1104 44-45 0.29293766505997354 1104 46-47 0.34492423109468007 1104 48-49 0.3063514544080661 1104 50-51 0.277310850094139 1104 52-53 0.2560914531451246 1104 54-55 0.20400795803347904 1104 56-57 0.1500368945911177 1104 58-59 0.1220887251250744 1104 60-61 0.1613949330076636 1104 62-63 0.15427925761800054 1104 64-65 0.15419141412477444 1104 66-67 0.07865062761886321 1104 68-69 0.06904104666254796 1104 70-71 0.06199005689291681 1104 72-73 0.04832350226092075 1104 74-75 0.03122811525248892 1104 76-77 0.1027438934359175 1104 78-79 0.06444966128222163 1104 80-81 0.07907049564424184 1104 82-83 -0.08212630257104436 1104 84-85 0.06088890350504883 1104 86-87 0.004152857507104812 1104 88-89 0.03177093749549087 1104 90-91 -0.08617978585548514 1104 92-93 -0.15725117433499136 1104 94-95 -0.30554914604869055 1104 96-97 -0.08690733411092921 1104 98-99 -0.18078739743525674 1104 100-101 -0.18886475008187986 1105 1 0.21882649597112547 1105 2 0.24543411047403652 1105 3 0.2973804597710057 1105 4 0.29259400253463497 1105 5 0.3525755769643979 1105 6 0.20182612941546552 1105 7 0.32362127020203957 1105 8 0.2438805252100238 1105 9 0.36477853460659304 1105 10-11 0.384866688813041 1105 12-13 0.29792993676017687 1105 14-15 0.36638063696130274 1105 16-17 0.39420467582999663 1105 18-19 0.32205089598453185 1105 20-21 0.4281537290229025 1105 22-23 0.42980559948740904 1105 24-25 0.34911420590581344 1105 26-27 0.2844655146626991 1105 28-29 0.26297943042995087 1105 30-31 0.4122010104333853 1105 32-33 0.31484236241460195 1105 34-35 0.45934829828953383 1105 36-37 0.4788153414034113 1105 38-39 0.6137696399090586 1105 40-41 0.6437052761226099 1105 42-43 0.524691069762774 1105 44-45 0.39553359176322544 1105 46-47 0.37523749283787566 1105 48-49 0.32661794100138053 1105 50-51 0.26183775756968686 1105 52-53 0.2411071703483998 1105 54-55 0.34900327581274126 1105 56-57 0.3395909542411957 1105 58-59 0.3166991125964884 1105 60-61 0.4231798943734617 1105 62-63 0.24445141746289778 1105 64-65 0.25866846418454514 1105 66-67 0.362884955120208 1105 68-69 0.368998459629708 1105 70-71 0.17183989642686015 1105 72-73 0.3206775769693948 1105 74-75 0.4494592475499388 1105 76-77 0.2737414182116886 1105 78-79 0.19415305789219772 1105 80-81 0.24572579008298945 1105 82-83 0.2839168915034769 1105 84-85 0.2548257805814629 1105 86-87 0.2310676269724361 1105 88-89 0.2382221671078355 1105 90-91 0.11832502812623602 1105 92-93 0.14536675174709757 1105 94-95 0.2648553366482673 1105 96-97 0.2892332219204299 1105 98-99 0.1919614896549895 1105 100-101 -0.27993747273441727 1106 1 0.02824314391695637 1106 2 0.17972924870679918 1106 3 0.06272369176311798 1106 4 0.22420956064397757 1106 5 0.1469570554211046 1106 6 0.172638332534774 1106 7 0.19742764458577255 1106 8 0.2444884590055807 1106 9 0.21979913833935427 1106 10-11 0.18053199757706295 1106 12-13 0.22833927830851763 1106 14-15 0.28672598163689855 1106 16-17 0.23008412392083244 1106 18-19 0.19780280449600696 1106 20-21 0.2631152749395014 1106 22-23 0.25300797347861703 1106 24-25 0.25488584631330014 1106 26-27 0.23994685370662694 1106 28-29 0.255545217609324 1106 30-31 0.186526594016577 1106 32-33 0.14721532363449086 1106 34-35 0.3113027129870076 1106 36-37 0.20787629617414183 1106 38-39 0.3149386310682232 1106 40-41 0.23391491433874734 1106 42-43 0.1647647419959597 1106 44-45 0.25866762997319626 1106 46-47 0.038572823961473546 1106 48-49 9.84075131235329E-4 1106 50-51 0.12675666864152646 1106 52-53 0.023497527752809333 1106 54-55 0.1281556720661925 1106 56-57 0.25738023575849667 1106 58-59 0.43605161768760325 1106 60-61 0.33122504173009304 1106 62-63 0.3696747495986443 1106 64-65 0.4136478685618883 1106 66-67 0.2688586183511603 1106 68-69 0.31676789570947506 1106 70-71 0.3291764229805736 1106 72-73 0.24176029278548583 1106 74-75 0.3002141770771374 1106 76-77 0.3246641459009858 1106 78-79 0.39799132933593384 1106 80-81 0.3054346858186143 1106 82-83 0.2825323336382546 1106 84-85 0.25940637033239256 1106 86-87 0.2262443827502807 1106 88-89 0.29413545057481016 1106 90-91 0.24357707004901386 1106 92-93 0.3144789137481503 1106 94-95 0.19265898116920255 1106 96-97 0.11071373859927292 1106 98-99 -0.020327191308716408 1106 100-101 -0.10980530228262708 1107 1 0.07815653265858558 1107 2 0.13921017470532604 1107 3 0.20829042929117492 1107 4 0.1430612937282234 1107 5 0.16582690736193229 1107 6 0.20047451932769178 1107 7 0.2310478077491993 1107 8 0.1880883634129944 1107 9 0.3011135989050686 1107 10-11 0.26958625574265227 1107 12-13 0.36330039305554607 1107 14-15 0.4839169340795806 1107 16-17 0.48972179364844237 1107 18-19 0.39703134731820455 1107 20-21 0.49141302398395936 1107 22-23 0.42840170716578996 1107 24-25 0.4151219849975263 1107 26-27 0.48139920501876077 1107 28-29 0.3967626485741391 1107 30-31 0.43098870608169904 1107 32-33 0.49049190017025524 1107 34-35 0.49091486011836594 1107 36-37 0.4915974407235737 1107 38-39 0.5339774882057284 1107 40-41 0.4411598016191576 1107 42-43 0.6104676052438833 1107 44-45 0.7314949682038261 1107 46-47 0.7042930407004917 1107 48-49 0.6211202945693373 1107 50-51 0.5931722746001356 1107 52-53 0.664205287777925 1107 54-55 0.7355825288975524 1107 56-57 0.7177899093535487 1107 58-59 0.5609837891232345 1107 60-61 0.5378752868813521 1107 62-63 0.6167081773270553 1107 64-65 0.6346669917654282 1107 66-67 0.5320101747765591 1107 68-69 0.6284335496986699 1107 70-71 0.6927250322802365 1107 72-73 0.5096735940567712 1107 74-75 0.5191313567209193 1107 76-77 0.505554219110536 1107 78-79 0.5729245941508339 1107 80-81 0.4248388878313669 1107 82-83 0.5763926185104147 1107 84-85 0.43957692187975894 1107 86-87 0.30057871001744374 1107 88-89 0.3711681597968379 1107 90-91 0.5063294649401229 1107 92-93 0.4479584995760817 1107 94-95 0.3370925872746291 1107 96-97 0.47559525533500846 1107 98-99 0.471137499569533 1107 100-101 0.32762419427869816 1108 1 0.10285983821396627 1108 2 0.18019257297260083 1108 3 0.07364695302876356 1108 4 0.12811267588413244 1108 5 0.2571592916135188 1108 6 0.13236000969400408 1108 7 0.18445187920541883 1108 8 0.1565466781989926 1108 9 0.27282686169372994 1108 10-11 0.157433753434276 1108 12-13 0.22089982755294812 1108 14-15 0.23529586899164912 1108 16-17 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-0.9372932291846077 2316 86-87 -0.6476143044173774 2316 88-89 -0.8532397449673077 2316 90-91 -0.9146162641551641 2316 92-93 -0.979035937894718 2316 94-95 -1.01635236425615 2316 96-97 -0.933987747042913 2316 98-99 -0.8182135841261449 2316 100-101 -0.49511550934221304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8171.0 3 2340.0 4 2343.0 5 2535.0 6 2963.0 7 3582.0 8 4371.0 9 5480.0 10 6742.0 11 8535.0 12 10887.0 13 14911.0 14 14278.0 15 15451.0 16 19413.0 17 24919.0 18 20807.0 19 15194.0 20 15090.0 21 16212.0 22 17614.0 23 19569.0 24 21912.0 25 25179.0 26 28997.0 27 34096.0 28 40361.0 29 48030.0 30 57702.0 31 70699.0 32 88282.0 33 113717.0 34 154396.0 35 221191.0 36 338960.0 37 537917.0 38 521042.0 39 83747.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.487207564817666 18.929153976890348 14.532655024390987 23.050983433901 2 15.152370845725372 20.12011738468382 40.83638105388127 23.891130715709537 3 19.14148336788196 24.604708912786762 29.779335428899607 26.474472290431667 4 12.85190396819944 16.44867426176322 37.04528398861381 33.65413778142353 5 13.856561227685562 37.569721644886066 33.8152967191489 14.758420408279473 6 32.02302600383135 35.69941012461354 17.65339605105931 14.624167820495797 7 28.739831430217897 32.85832270145652 20.523638427415484 17.878207440910096 8 26.420173129986345 35.17167123892613 19.753147309208547 18.655008321878974 9 26.94886884419975 16.921659797381373 20.39168583794152 35.73778552047735 10-11 25.13580281571523 26.74036064614788 27.111094081884197 21.012742456252695 12-13 25.939067123360687 24.050642275761167 27.78739182302215 22.222898777855992 14-15 22.874863998276172 25.296605154180813 26.234125544955305 25.594405302587713 16-17 22.249102373860506 28.662920685490654 26.794944044540543 22.293032896108297 18-19 22.134270612658657 27.06676692505205 29.367385775247207 21.431576687042085 20-21 22.948757250258748 26.226530559594014 29.279891098154316 21.544821091992922 22-23 22.684611123337152 26.240087420414792 29.128983054706058 21.946318401541998 24-25 22.296622212609165 26.382961365003293 29.302680334913795 22.017736087473747 26-27 22.131625291854927 26.67287469087948 29.15536339519728 22.040136622068314 28-29 21.994516728606904 26.785501867693075 29.342844095579895 21.877137308120126 30-31 22.18647199657 26.51902624479714 29.25968576057551 22.034815998057347 32-33 21.980365480091933 26.614578077113066 29.106111847561582 22.298944595233415 34-35 22.52274934974712 26.717032444996434 28.8868286357535 21.873389569502947 36-37 22.484457301244102 26.477973851583116 28.877150843898715 22.16041800327407 38-39 22.227241653868703 26.73284200811588 29.003341229586184 22.036575108429236 40-41 22.37434707414286 26.543449593724453 29.052324212561814 22.029879119570868 42-43 22.402746060717313 26.543218213481683 28.723365198873495 22.330670526927506 44-45 22.286773811270027 26.899044777266578 28.544472962815203 22.269708448648192 46-47 22.340593527431494 27.005178496207925 28.15269352960046 22.501534446760118 48-49 22.47067865087639 27.02987039601208 28.060891212224366 22.43855974088716 50-51 22.47496378277198 27.18611450313997 27.9050364683798 22.433885245708254 52-53 22.66008568142917 27.21074145868485 27.793665145284745 22.33550771460123 54-55 22.268171777777905 27.078854345552706 28.022621183074865 22.630352693594528 56-57 22.3620912350027 27.41268658121607 27.8401170702686 22.385105113512626 58-59 22.452096029867135 26.98531840610487 28.04303669742392 22.519548866604076 60-61 22.503545582957326 26.9475900015416 28.228847064432188 22.320017351068884 62-63 22.412901026591552 27.009108603220692 28.20047068598716 22.377519684200596 64-65 22.255203888538457 26.832456623558993 28.197288716749963 22.715050771152587 66-67 22.1827757065027 27.239369944190695 27.913195592113517 22.66465875719309 68-69 22.239125974910298 27.082780641519616 28.066625991086642 22.61146739248344 70-71 22.148404589477938 27.171773899946732 27.940175935443268 22.739645575132062 72-73 22.25737539561591 27.116308461390187 27.869379628204292 22.756936514789608 74-75 22.22986119502247 27.235498452489576 27.69700193046336 22.837638422024597 76-77 22.29959747407706 27.032021520306376 27.79510275664714 22.873278248969427 78-79 22.29135645977246 27.15044232301183 27.760838188221815 22.7973630289939 80-81 22.163989943122875 27.416421895810355 27.68890026778014 22.73068789328663 82-83 22.34665226925668 27.309573421559325 27.59978548467888 22.743988824505124 84-85 22.292076541562434 27.115125689260466 27.550232802281965 23.042564966895142 86-87 22.33773789000568 26.976847446393304 27.7988922721965 22.88652239140451 88-89 22.35439706119908 26.87601710051222 27.75990634255936 23.009679495729344 90-91 22.29336479812734 27.030211911581393 27.824780428390632 22.85164286190064 92-93 22.426121815152534 26.795299123747686 27.800158522788365 22.97842053831142 94-95 22.294101381839525 26.68328256394574 27.788632389353214 23.233983664861526 96-97 22.43376276662273 26.580343559319004 27.709618496220966 23.2762751778373 98-99 22.237614609591443 26.62424837629871 27.611247158249196 23.526889855860652 100-101 22.51149596951404 26.150120144058718 28.131422189837124 23.206961696590113 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2109.0 1 1216.5 2 408.0 3 502.5 4 450.0 5 453.0 6 750.5 7 1596.0 8 2609.5 9 3047.5 10 2945.0 11 2874.0 12 3071.5 13 3311.5 14 3595.5 15 3958.0 16 4241.0 17 4355.0 18 4620.0 19 4885.5 20 5073.0 21 5633.0 22 6618.0 23 7728.5 24 9108.0 25 11266.0 26 14067.0 27 17366.5 28 20989.5 29 24758.5 30 29215.0 31 34377.5 32 39992.0 33 45036.0 34 49247.0 35 53667.5 36 59616.5 37 65989.5 38 70348.0 39 73082.0 40 75546.0 41 80804.0 42 85975.0 43 89896.0 44 94866.5 45 100053.5 46 100997.0 47 100939.0 48 106994.5 49 107903.5 50 103210.0 51 99373.0 52 95044.0 53 93047.0 54 89050.5 55 80376.0 56 71245.5 57 63761.5 58 56966.5 59 49246.0 60 41414.5 61 34170.0 62 27806.0 63 21862.5 64 16866.5 65 13328.0 66 10450.5 67 8548.5 68 6582.5 69 4407.0 70 3187.5 71 2440.5 72 1969.5 73 1625.0 74 1248.5 75 977.5 76 720.5 77 483.5 78 323.5 79 226.0 80 155.5 81 108.0 82 77.5 83 68.5 84 55.5 85 35.5 86 21.0 87 16.0 88 13.0 89 12.0 90 12.0 91 9.5 92 5.0 93 5.5 94 5.0 95 5.0 96 4.5 97 2.5 98 3.5 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.288435510323853 2 0.11077925357075429 3 0.12234245423436826 4 0.12355564250071466 5 0.13091059636543959 6 0.05792973971803988 7 0.06585337558261471 8 0.07032700731476701 9 0.06786271864875092 10-11 0.06395776891644848 12-13 0.06738881698220936 14-15 0.06376820824983186 16-17 0.06435584631634339 18-19 0.0690759069150973 20-21 0.07241217464754984 22-23 0.08105614104526784 24-25 0.08996549237624911 26-27 0.09019296517618905 28-29 0.08369103431123891 30-31 0.0799187770455681 32-33 0.07248799891419651 34-35 0.24241018046933707 36-37 0.36987077270235413 38-39 0.3413987605765374 40-41 0.32418665204774805 42-43 0.3482419006413975 44-45 0.36196609290444093 46-47 0.3675581325696313 48-49 0.3769413855671542 50-51 0.3709702245687305 52-53 0.3716905551018737 54-55 0.35764410970558197 56-57 0.33474518117829394 58-59 0.3197319763822574 60-61 0.2764742322603442 62-63 0.34780591110817927 64-65 0.37743424330035735 66-67 0.38175622649921637 68-69 0.3937175045627252 70-71 0.3945705275625 72-73 0.37804083743353056 74-75 0.38179413863253964 76-77 0.3657952183700967 78-79 0.34333227937602695 80-81 0.3423086517762972 82-83 0.357416636905642 84-85 0.3635584025040206 86-87 0.3569427352391004 88-89 0.371671599035212 90-91 0.39439992296254506 92-93 0.39032436863028774 94-95 0.3895282138304979 96-97 0.3949306928290717 98-99 0.38353809676541256 100-101 0.36331197363741896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2637678.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.61472445454295 #Duplication Level Percentage of deduplicated Percentage of total 1 71.18238210417489 35.316942741166265 2 14.867074904322664 14.752516496460387 3 5.670173389845478 8.439722710399954 4 2.7806684358129563 5.518483929692191 5 1.5477260761077756 3.8395001398599122 6 0.9617686784458392 2.8630732782060146 7 0.5844562035583806 2.029834344670812 8 0.43143127203873155 1.7124274946619702 9 0.309679344483785 1.382818981124383 >10 1.4989715741808458 13.358838644896037 >50 0.10422086578354409 3.591913060491404 >100 0.05747199142076102 5.055083083110152 >500 0.003363596774409143 1.0874004979664929 >1k 4.5867228741550993E-4 0.42703321264461563 >5k 1.5289076247183665E-4 0.6244113846494633 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9336 0.35394767670655786 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7127 0.2701997741953339 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4933 0.1870205536839599 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 3.791213332332453E-5 0.0 0.0 0.0 0.0 12-13 3.791213332332453E-5 0.0 0.0 3.791213332332453E-5 0.0 14-15 3.791213332332453E-5 0.0 0.0 3.791213332332453E-5 0.0 16-17 3.791213332332453E-5 0.0 0.0 3.791213332332453E-5 0.0 18-19 3.791213332332453E-5 0.0 0.0 7.582426664664905E-5 0.0 20-21 3.791213332332453E-5 0.0 0.0 1.5164853329329813E-4 0.0 22-23 3.791213332332453E-5 0.0 0.0 3.4120919990992074E-4 0.0 24-25 3.791213332332453E-5 0.0 0.0 5.118137998648812E-4 0.0 26-27 3.791213332332453E-5 0.0 0.0 9.288472664214509E-4 0.0 28-29 3.791213332332453E-5 0.0 0.0 0.002236815866076147 0.0 30-31 3.791213332332453E-5 0.0 0.0 0.006805227931536753 0.0 32-33 3.791213332332453E-5 0.0 0.0 0.016207436995721237 0.0 34-35 3.791213332332453E-5 0.0 0.0 0.030386574858644613 0.0 36-37 3.791213332332453E-5 0.0 0.0 0.05546545105202379 0.0 38-39 3.791213332332453E-5 0.0 0.0 0.09428747557510811 0.0 40-41 3.791213332332453E-5 0.0 0.0 0.13563065696419352 0.0 42-43 3.791213332332453E-5 0.0 0.0 0.177978509886347 0.0 44-45 3.791213332332453E-5 0.0 0.0 0.23441072033811558 0.0 46-47 3.791213332332453E-5 0.0 0.0 0.3034676711865512 0.0 48-49 3.791213332332453E-5 0.0 0.0 0.3790644650332603 0.0 50-51 3.791213332332453E-5 0.0 0.0 0.4628502796778075 0.0 52-53 3.791213332332453E-5 0.0 0.0 0.548247359988596 0.0 54-55 3.791213332332453E-5 0.0 0.0 0.6467620384292548 0.0 56-57 3.791213332332453E-5 0.0 0.0 0.7665075115309754 0.0 58-59 3.791213332332453E-5 0.0 0.0 0.8979109656296181 0.0 60-61 3.791213332332453E-5 0.0 0.0 1.0459388901905389 0.0 62-63 3.791213332332453E-5 0.0 0.0 1.2020420991493275 0.0 64-65 3.791213332332453E-5 0.0 0.0 1.3677939460389026 0.0 66-67 3.791213332332453E-5 0.0 0.0 1.5597430770548946 0.0 68-69 3.791213332332453E-5 0.0 0.0 1.7754820717312727 0.0 70-71 3.791213332332453E-5 0.0 0.0 2.021910938332882 0.0 72-73 3.791213332332453E-5 0.0 0.0 2.2699889827340565 0.0 74-75 3.791213332332453E-5 0.0 0.0 2.5337626503310866 0.0 76-77 3.791213332332453E-5 0.0 0.0 2.8246814053876177 0.0 78-79 3.791213332332453E-5 0.0 0.0 3.1544790531672176 0.0 80-81 3.791213332332453E-5 0.0 0.0 3.5147580561387706 0.0 82-83 3.791213332332453E-5 0.0 0.0 3.8944101592385425 0.0 84-85 3.791213332332453E-5 0.0 0.0 4.28655431026835 0.0 86-87 3.791213332332453E-5 0.0 0.0 4.70792871609044 0.0 88-89 5.6868199984986794E-5 0.0 0.0 5.155765790972211 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6690 0.0 40.543755 1 GTATCAA 11045 0.0 38.578087 1 ATCAACG 13875 0.0 30.51979 3 TATCAAC 13905 0.0 30.488047 2 TCAACGC 14135 0.0 29.958408 4 CAACGCA 14160 0.0 29.837973 5 AACGCAG 14615 0.0 28.98822 6 GTACATG 12650 0.0 25.42214 1 ACGCAGA 16650 0.0 25.35983 7 CGCAGAG 16915 0.0 24.934511 8 TACATGG 12725 0.0 24.930525 2 ACATGGG 13055 0.0 23.174313 3 GCAGAGT 18045 0.0 23.110025 9 GAGTACT 8880 0.0 22.33394 12-13 CATGGGG 6185 0.0 21.390827 4 AGTACTT 9330 0.0 21.028173 12-13 GTACTTT 9605 0.0 20.377167 14-15 AGAGTAC 16700 0.0 19.267797 10-11 CAGAGTA 17540 0.0 19.169092 10-11 CATGGGA 7150 0.0 18.238525 4 >>END_MODULE