FastQCFastQC Report
Fri 27 May 2016
SRR939352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637678
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95700.3628191159042158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83190.31539103711673677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53150.20150298861346988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74350.044.9945261
GTATCAA116650.035.776691
TATCAAC148600.028.0674022
ATCAACG148050.027.9793743
TCAACGC150650.027.5594844
CAACGCA152650.027.1984045
AACGCAG157700.026.3575216
GTACATG127350.024.5500141
TACATGG130950.023.443962
ACGCAGA177200.023.3766737
CGCAGAG179400.023.0371048
GAGTACT97800.022.77999512-13
ACATGGG134150.021.8943543
GTACTTT104100.021.19625314-15
GCAGAGT196150.020.9255289
GGACCGA2751.2769306E-920.7052616
CATGGGG64400.020.0408084
AGTACTT103200.019.77177412-13
CAGAGTA189850.019.13318810-11
AGAGTAC182500.018.82471710-11
Produced by FastQC (version 0.11.5)