##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939352_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2637678 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23376735143562 33.0 31.0 34.0 31.0 34.0 2 32.43980955977189 34.0 31.0 34.0 31.0 34.0 3 32.5250174585374 34.0 31.0 34.0 31.0 34.0 4 36.07648886634381 37.0 35.0 37.0 35.0 37.0 5 36.01195710772884 37.0 35.0 37.0 35.0 37.0 6 36.051796693910326 37.0 35.0 37.0 35.0 37.0 7 35.99351626695905 37.0 35.0 37.0 35.0 37.0 8 35.97421519988414 37.0 35.0 37.0 35.0 37.0 9 37.67140492508942 39.0 37.0 39.0 35.0 39.0 10-11 37.59074477627671 39.0 37.0 39.0 35.0 39.0 12-13 37.50271431918529 39.0 37.0 39.0 35.0 39.0 14-15 38.78293787187064 40.0 38.0 41.0 34.5 41.0 16-17 38.723146456845754 40.0 38.0 41.0 34.0 41.0 18-19 38.845155284306884 40.0 38.0 41.0 35.0 41.0 20-21 38.859710510532366 40.0 38.0 41.0 34.5 41.0 22-23 38.79992914222282 40.0 38.0 41.0 34.0 41.0 24-25 38.73194093441277 40.0 38.0 41.0 34.0 41.0 26-27 38.5636133371852 40.0 38.0 41.0 34.0 41.0 28-29 38.412993928750964 40.0 38.0 41.0 34.0 41.0 30-31 38.21575927766771 40.0 38.0 41.0 34.0 41.0 32-33 38.008364174853796 40.0 37.0 41.0 33.0 41.0 34-35 37.96748863962925 40.0 37.0 41.0 33.0 41.0 36-37 37.88263029073299 40.0 37.0 41.0 33.0 41.0 38-39 37.77911253761832 40.0 37.0 41.0 33.0 41.0 40-41 37.59573609818939 40.0 37.0 41.0 33.0 41.0 42-43 37.40952003997455 40.0 37.0 41.0 32.0 41.0 44-45 37.16660088911535 40.0 36.5 41.0 31.5 41.0 46-47 36.853290090754065 40.0 36.0 41.0 31.0 41.0 48-49 36.536614969681665 39.5 36.0 41.0 30.0 41.0 50-51 36.284915747866116 39.0 35.5 41.0 30.0 41.0 52-53 36.05331469572859 39.0 35.0 41.0 29.0 41.0 54-55 35.772961673107936 39.0 35.0 40.0 28.5 41.0 56-57 35.531779656197614 38.5 35.0 40.0 28.0 41.0 58-59 35.23044947108783 38.0 34.5 40.0 27.5 41.0 60-61 34.90223200102514 38.0 34.0 40.0 27.0 41.0 62-63 34.51679602286556 37.5 34.0 40.0 26.0 41.0 64-65 34.08920421673912 37.0 33.5 39.5 26.0 41.0 66-67 33.71215819368399 36.0 33.0 39.0 25.5 40.5 68-69 33.32767987601216 36.0 33.0 39.0 25.0 40.0 70-71 32.93176782761201 35.0 32.5 38.5 24.0 40.0 72-73 32.43118834065417 35.0 32.0 37.5 22.5 40.0 74-75 31.920641753845615 35.0 31.0 37.0 21.5 39.0 76-77 29.922115967149892 33.0 29.0 35.0 18.0 37.5 78-79 30.91318955535892 34.0 30.5 36.0 19.5 38.0 80-81 30.962523287527894 34.0 31.0 36.0 19.5 37.0 82-83 30.64964942650316 34.0 31.0 35.0 18.0 37.0 84-85 30.271849141555563 34.0 30.5 35.0 15.0 36.5 86-87 29.724807197845983 34.0 30.0 35.0 7.5 36.0 88-89 29.21967654884334 34.0 29.0 35.0 2.0 36.0 90-91 28.791111917375815 34.0 29.0 35.0 2.0 35.0 92-93 28.423321572989575 34.0 29.0 35.0 2.0 35.0 94-95 28.00843886175644 33.5 28.5 35.0 2.0 35.0 96-97 27.44077158015497 33.0 27.0 35.0 2.0 35.0 98-99 26.663679569682124 33.0 25.5 35.0 2.0 35.0 100-101 23.29212947903421 29.0 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1902008580862784 1101 2 0.18543915441052405 1101 3 0.15424924124175732 1101 4 0.13056124410726966 1101 5 0.193408091867326 1101 6 0.10842151497271857 1101 7 0.09040521374244292 1101 8 0.19062547358011273 1101 9 0.25627461530265805 1101 10-11 0.20566205421363293 1101 12-13 0.19202212018713283 1101 14-15 0.20168687609565694 1101 16-17 0.2789161256671093 1101 18-19 0.2201698672824648 1101 20-21 0.24988049224720754 1101 22-23 0.27355153573972046 1101 24-25 0.23695231354704305 1101 26-27 0.2790734044816716 1101 28-29 0.2750310029630185 1101 30-31 0.28639495392183534 1101 32-33 0.22442629096961042 1101 34-35 0.2898923699230025 1101 36-37 0.36974143231481094 1101 38-39 0.3814534066601354 1101 40-41 0.4877801046437682 1101 42-43 0.495750498778186 1101 44-45 0.6419060860008798 1101 46-47 0.5584358232804334 1101 48-49 0.576612579705511 1101 50-51 0.5402434330944317 1101 52-53 0.5584828763282843 1101 54-55 0.5219949511316528 1101 56-57 0.5745447127946264 1101 58-59 0.5923025028194573 1101 60-61 0.4387728914566864 1101 62-63 0.4199619708803155 1101 64-65 0.4269599898700136 1101 66-67 0.5567019798196071 1101 68-69 0.5650764984036769 1101 70-71 0.5625352592230541 1101 72-73 0.4213672376020199 1101 74-75 0.3632955608613777 1101 76-77 0.4976834529820131 1101 78-79 0.7324531019907958 1101 80-81 0.7483879715220709 1101 82-83 0.6177572539608356 1101 84-85 0.5919648772774728 1101 86-87 0.7211883770244363 1101 88-89 0.8665492884171613 1101 90-91 0.9201936276850553 1101 92-93 0.8324931516919669 1101 94-95 0.9334460306945616 1101 96-97 0.7249997711326088 1101 98-99 0.7664147790530116 1101 100-101 0.7005470918958316 1102 1 0.06013263592131324 1102 2 0.1390692582790365 1102 3 0.10916190769431466 1102 4 0.08611536831890021 1102 5 0.09770153541022353 1102 6 0.06941929501711996 1102 7 0.15305101964985823 1102 8 0.20288499309560848 1102 9 0.20078984264516464 1102 10-11 0.18625473647763613 1102 12-13 0.22803181175800802 1102 14-15 0.3052618340082631 1102 16-17 0.29888416748274693 1102 18-19 0.32188856820256717 1102 20-21 0.2442610752237826 1102 22-23 0.23430579124284634 1102 24-25 0.27643999438166134 1102 26-27 0.3023344980833258 1102 28-29 0.3915048205454923 1102 30-31 0.274009436926292 1102 32-33 0.28608582248487124 1102 34-35 0.4076592498795222 1102 36-37 0.4381391820068714 1102 38-39 0.23195342607150593 1102 40-41 0.2745905154943742 1102 42-43 0.3037080160984331 1102 44-45 0.3136890915172472 1102 46-47 0.2918199497155527 1102 48-49 0.28298012882512324 1102 50-51 0.22695096364969913 1102 52-53 0.27829562713208844 1102 54-55 0.3152036163701126 1102 56-57 0.313678400709108 1102 58-59 0.24392185278539813 1102 60-61 0.0882987244694462 1102 62-63 0.06049618960770431 1102 64-65 0.2231972710067467 1102 66-67 0.18917791853611732 1102 68-69 0.30075619657441877 1102 70-71 0.24993577591860117 1102 72-73 0.4624951797490624 1102 74-75 0.3806541842771374 1102 76-77 0.4503893106295642 1102 78-79 0.47685327281091006 1102 80-81 0.39639133498420875 1102 82-83 0.3800684783245849 1102 84-85 0.4017970923978744 1102 86-87 0.5850475704288023 1102 88-89 0.6598850981715536 1102 90-91 0.7488771128388798 1102 92-93 0.6229607541046285 1102 94-95 0.6447901273420413 1102 96-97 0.44505216420239435 1102 98-99 0.4361595900391464 1102 100-101 0.3997241377666789 1103 1 0.11734423508518432 1103 2 0.1518019640491559 1103 3 0.17620992890613962 1103 4 0.08286415130455538 1103 5 0.08310928782671567 1103 6 0.16241953350984772 1103 7 0.17118348131565853 1103 8 0.1302478247057124 1103 9 0.1777297003912679 1103 10-11 0.15947726421994446 1103 12-13 0.22142696614697144 1103 14-15 0.20351241971833645 1103 16-17 0.23619530228724983 1103 18-19 0.18162328011737827 1103 20-21 0.15080419330573847 1103 22-23 0.16444180224156923 1103 24-25 0.18657242798949625 1103 26-27 0.2738893303668277 1103 28-29 0.2616272935747759 1103 30-31 0.2393010293562483 1103 32-33 0.25833894744102537 1103 34-35 0.3377731652286329 1103 36-37 0.35271319191133443 1103 38-39 0.2542202292869504 1103 40-41 0.2414814120867348 1103 42-43 0.24863723987474629 1103 44-45 0.47719268995161457 1103 46-47 0.4189060596186138 1103 48-49 0.23924968172140382 1103 50-51 0.3265711289111337 1103 52-53 0.19357669782414888 1103 54-55 0.24108954670217742 1103 56-57 0.25251939620579833 1103 58-59 0.06504424164077705 1103 60-61 -0.05416209959307139 1103 62-63 -0.030401805650505764 1103 64-65 0.10155475757398591 1103 66-67 0.13542025520368384 1103 68-69 -0.008489955976749286 1103 70-71 0.16094993116334422 1103 72-73 0.06983654701382846 1103 74-75 0.20043961096869012 1103 76-77 0.15770051557899123 1103 78-79 0.37953736815188677 1103 80-81 0.39312082811702354 1103 82-83 0.2830282431988138 1103 84-85 0.2893730242731287 1103 86-87 0.4487454702899143 1103 88-89 0.5716459460833399 1103 90-91 0.4796675759356397 1103 92-93 0.40023380021789734 1103 94-95 0.17468799633939014 1103 96-97 0.2156631965953153 1103 98-99 0.16274626811605586 1103 100-101 0.258718820781791 1104 1 0.0021543283192002605 1104 2 0.017968027088883787 1104 3 0.02065790144065005 1104 4 -0.027623150344375347 1104 5 0.05267026708893496 1104 6 0.022160158804496177 1104 7 0.1046828351667628 1104 8 0.03373314631038227 1104 9 0.015598324177318545 1104 10-11 0.013849030008998398 1104 12-13 0.05187146389793185 1104 14-15 0.06060431983980408 1104 16-17 0.0016543453213202497 1104 18-19 -3.2171125805291467E-4 1104 20-21 0.022764104342407165 1104 22-23 0.06442842169037277 1104 24-25 0.016031063367393017 1104 26-27 0.031042725560368467 1104 28-29 0.00609100385602801 1104 30-31 0.10985563587098568 1104 32-33 0.03474959031758118 1104 34-35 0.04416990065146109 1104 36-37 -0.010964296593868994 1104 38-39 0.07201351987647087 1104 40-41 0.254832844984314 1104 42-43 0.3380054560046233 1104 44-45 0.44681641221296786 1104 46-47 0.30188932630339593 1104 48-49 0.32176261856519517 1104 50-51 0.22906157533623883 1104 52-53 0.09558114599079914 1104 54-55 0.19492317861361386 1104 56-57 0.19342727700380635 1104 58-59 0.2727180224160506 1104 60-61 0.2780461350615866 1104 62-63 0.30877887477479504 1104 64-65 0.25162457215976275 1104 66-67 0.2315758079244361 1104 68-69 0.2615975098439165 1104 70-71 0.10017761684102311 1104 72-73 0.14691476153526395 1104 74-75 0.02527017020639377 1104 76-77 0.2390130159256394 1104 78-79 0.4090914006201736 1104 80-81 0.4032068518886476 1104 82-83 0.31309808551629814 1104 84-85 0.385852691381654 1104 86-87 0.1821137224710121 1104 88-89 0.3969649301043283 1104 90-91 0.4886318519438255 1104 92-93 0.5297738281714608 1104 94-95 0.48578338511026686 1104 96-97 0.48099363381349036 1104 98-99 0.6221865589662521 1104 100-101 0.5667775870533696 1105 1 0.10951012062623988 1105 2 0.07189473974641203 1105 3 0.09464051840205201 1105 4 0.05824501337806254 1105 5 0.12151185273828702 1105 6 0.057946207198419586 1105 7 0.09091851792395289 1105 8 0.1279022521238602 1105 9 0.13641961863488206 1105 10-11 0.08184475718846329 1105 12-13 0.06888333650388034 1105 14-15 0.1293453200939112 1105 16-17 0.08601666920863948 1105 18-19 0.08676406514355506 1105 20-21 0.11579677165532587 1105 22-23 0.10072210280996075 1105 24-25 0.12009224281644748 1105 26-27 0.17100936179652138 1105 28-29 0.08090242094542788 1105 30-31 0.17044098404939234 1105 32-33 0.029375036187339276 1105 34-35 0.1398860216080422 1105 36-37 0.19337640437457537 1105 38-39 0.06473784295440765 1105 40-41 0.11311802281458938 1105 42-43 0.09166299979162318 1105 44-45 0.2333994969258697 1105 46-47 0.24241806431550117 1105 48-49 0.2540998231617735 1105 50-51 0.36834823077185064 1105 52-53 0.3792472542418608 1105 54-55 0.25882372730401215 1105 56-57 0.2216770675979518 1105 58-59 0.1900909436512066 1105 60-61 0.042603959311250605 1105 62-63 0.08348700066300552 1105 64-65 0.1966784765201126 1105 66-67 0.2530410841989834 1105 68-69 0.4167190122956086 1105 70-71 0.31033344773520355 1105 72-73 0.27644404541430134 1105 74-75 0.34358000616396467 1105 76-77 0.4136710255818379 1105 78-79 0.3206697795835929 1105 80-81 0.3559070109429783 1105 82-83 0.33282635201230093 1105 84-85 0.3462908672449281 1105 86-87 0.31178359061591365 1105 88-89 0.5383297621561276 1105 90-91 0.5324953193282731 1105 92-93 0.4032497535571018 1105 94-95 0.3323106596342775 1105 96-97 0.06748354468659556 1105 98-99 0.24219846451245175 1105 100-101 0.4252645238927997 1106 1 0.08723894938858479 1106 2 0.03717471288572938 1106 3 0.054487853703982125 1106 4 0.05643499917725592 1106 5 0.08322889264569966 1106 6 0.02629017964351732 1106 7 0.06272583145834432 1106 8 0.022989066789207868 1106 9 0.0695525782003088 1106 10-11 0.04618047029821071 1106 12-13 0.09957742399087266 1106 14-15 0.10150347695802253 1106 16-17 0.023914480209036526 1106 18-19 -0.03146056704271416 1106 20-21 0.05675833865821289 1106 22-23 0.05016692527561872 1106 24-25 0.06508956242531383 1106 26-27 0.11406341810825182 1106 28-29 0.1809724111889608 1106 30-31 0.2272520566194558 1106 32-33 0.18816813837715785 1106 34-35 0.08908681088387738 1106 36-37 0.1873055820338223 1106 38-39 0.3144532997684806 1106 40-41 0.2675794345088818 1106 42-43 0.2744945471437106 1106 44-45 0.3324210933360092 1106 46-47 0.4867800379592637 1106 48-49 0.23106411191735532 1106 50-51 0.3530742185628881 1106 52-53 0.4684646205811802 1106 54-55 0.45771637297576007 1106 56-57 0.45360194527722797 1106 58-59 0.4989710267636056 1106 60-61 0.34865304655900076 1106 62-63 0.468361079050446 1106 64-65 0.41148940148618607 1106 66-67 0.4397193375085209 1106 68-69 0.4381764151628573 1106 70-71 0.483574060891911 1106 72-73 0.4773458895720637 1106 74-75 0.39907042853035435 1106 76-77 0.4835939552128501 1106 78-79 0.7816898366949836 1106 80-81 0.7017857372340899 1106 82-83 0.6671434686413527 1106 84-85 0.6604311672703069 1106 86-87 0.5699754092998219 1106 88-89 0.7850375038231938 1106 90-91 0.8528977591735654 1106 92-93 0.7875352813103014 1106 94-95 0.8815901980717342 1106 96-97 0.7654345255637693 1106 98-99 0.8479129286491407 1106 100-101 0.8948492619646551 1107 1 -0.024515346420493245 1107 2 0.011497325374932643 1107 3 0.08720051206825019 1107 4 0.018526602493011524 1107 5 0.10361729220397109 1107 6 0.09699417531997767 1107 7 0.12043314007402017 1107 8 0.09324961907070417 1107 9 0.1182954263283662 1107 10-11 0.0784375799969581 1107 12-13 0.17718689078103722 1107 14-15 0.20887550862853743 1107 16-17 0.11097816330398302 1107 18-19 0.16376144361929335 1107 20-21 0.1503985419018008 1107 22-23 0.1437391047085299 1107 24-25 0.17092508381883675 1107 26-27 0.23411646262285046 1107 28-29 0.26980033984440865 1107 30-31 0.18325509933779216 1107 32-33 0.22649498693007075 1107 34-35 0.22373600014314832 1107 36-37 0.27093816286188144 1107 38-39 0.19783744920531632 1107 40-41 0.32151981722286394 1107 42-43 0.3012084582082437 1107 44-45 0.22177462502916967 1107 46-47 0.19228973232851843 1107 48-49 0.3770288395019108 1107 50-51 0.3520806491950026 1107 52-53 0.35152489545672694 1107 54-55 0.28007160565467615 1107 56-57 0.3264688013588426 1107 58-59 0.2785847358653655 1107 60-61 0.2011562603130841 1107 62-63 0.1570171128639899 1107 64-65 0.2966891853094822 1107 66-67 0.24233883995847805 1107 68-69 0.3857035036344243 1107 70-71 0.32697504836959723 1107 72-73 0.3369428699709047 1107 74-75 0.37712958659706786 1107 76-77 0.41374381065248755 1107 78-79 0.6046685298568306 1107 80-81 0.5192839448730595 1107 82-83 0.5785397608360263 1107 84-85 0.5398899289089449 1107 86-87 0.5878279499227652 1107 88-89 0.6700344657907777 1107 90-91 0.8245823598636441 1107 92-93 0.9206126597616695 1107 94-95 0.9775591429763537 1107 96-97 0.8120735557675935 1107 98-99 1.0194778043084867 1107 100-101 0.904891091531816 1108 1 -0.015486979013267899 1108 2 -0.03834803775330187 1108 3 0.055550589749415735 1108 4 0.010405337471119935 1108 5 -0.025359841039090725 1108 6 0.05507507631698161 1108 7 0.06189050481101077 1108 8 0.0036615062325040526 1108 9 0.03378633238030204 1108 10-11 0.12981393230786153 1108 12-13 0.05578417852749595 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12 5480.0 13 7676.0 14 9481.0 15 13026.0 16 17228.0 17 23878.0 18 22280.0 19 15132.0 20 13961.0 21 14816.0 22 15697.0 23 17179.0 24 19659.0 25 23544.0 26 27806.0 27 34508.0 28 41798.0 29 51083.0 30 62401.0 31 75920.0 32 94107.0 33 120183.0 34 163214.0 35 231813.0 36 360414.0 37 594287.0 38 509909.0 39 42425.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.28670147425986 19.7150937793815 15.137463126395057 22.860741619963584 2 15.60160110521451 20.751433647321623 39.93084068639159 23.716124561072277 3 19.064647011500266 24.862890769836195 30.142875665642283 25.929586553021256 4 13.146335526929368 17.13196227894383 36.611974623134444 33.10972757099236 5 13.707586748647863 37.775573819093914 33.85030318333019 14.666536248928033 6 31.91905911184004 36.20040050377643 17.257072318910797 14.62346806547274 7 28.46416431421879 33.180206226840426 20.407153564612514 17.94847589432827 8 26.364590370773083 35.7444312762968 19.368247375153448 18.522730977776668 9 26.585201074581505 17.003288498444466 20.246974801321464 36.16453562565256 10-11 25.071540195581115 27.00206393653812 26.885578906902207 21.04081696097856 12-13 25.874007365569263 24.281299688589737 27.711665335950787 22.133027609890213 14-15 22.69205323748917 25.507892546865257 26.19466828934473 25.605385926300844 16-17 22.001420576522044 28.987324567614152 26.79987557160007 22.21137928426373 18-19 21.950253215138467 27.163797097295422 29.461822102622083 21.42412758494403 20-21 22.77453881785419 26.319266415385044 29.43287618882972 21.473318577931046 22-23 22.484397261530788 26.311475471987105 29.288051839534624 21.91607542694749 24-25 22.1523101288918 26.455432857125256 29.459344442222374 21.932912571760575 26-27 21.934054810256875 26.788877957420347 29.34682263913453 21.93024459318824 28-29 21.847226992832333 26.81891042045314 29.549304350265647 21.784558236448877 30-31 22.109522087229752 26.52410567173097 29.43149239596342 21.93487984507586 32-33 21.861064921495345 26.660627263828264 29.28031776433666 22.19799005033973 34-35 22.34880660966894 26.685283405441446 29.131550178088677 21.834359806800943 36-37 22.331369975682072 26.494123009355935 29.114869884105904 22.05963713085609 38-39 22.114094061515644 26.730743565517145 29.21335214907405 21.94181022389316 40-41 22.26207893943045 26.58711838904879 29.18483049129871 21.96597218022205 42-43 22.243443531643784 26.59991401104359 28.88089619601236 22.27574626130027 44-45 22.18771361206703 26.843437780432485 28.73827514997155 22.230573457528948 46-47 22.241230962641158 27.081610304224306 28.283027991881486 22.394130741253047 48-49 22.39414742815461 27.082494527383556 28.106633940913184 22.416724103548653 50-51 22.41755817048176 27.251810114805526 27.9362378576915 22.394393857021214 52-53 22.655892796618847 27.288148894595928 27.754619783006113 22.301338525779112 54-55 22.260241773256627 27.12359127990604 27.96859586348296 22.647571083354375 56-57 22.318702282841198 27.519299171468237 27.760268690871285 22.401729854819276 58-59 22.407411409266462 27.07245011967033 28.010212070306178 22.50992640075703 60-61 22.404423067481314 27.070151896032286 28.203917423486608 22.321507612999795 62-63 22.37636052097359 27.10610267479793 28.109613959608186 22.40792284462029 64-65 22.192914031377985 26.957901411374213 28.123244786369828 22.72593977087798 66-67 22.126411942625293 27.321587396187102 27.841211095516588 22.710789565671018 68-69 22.154015826124933 27.13452130369903 28.014523457119633 22.696939413056402 70-71 22.081433382208402 27.249464727966178 27.856248536828325 22.8128533529971 72-73 22.197137027339956 27.179189423424692 27.72375930648093 22.899914242754424 74-75 22.163963910682046 27.343822862381227 27.506920859938173 22.98529236699855 76-77 22.1586209844818 27.12180385413747 27.77376773525486 22.945807426125864 78-79 22.135889975956125 27.153257524231538 27.715797758483028 22.995054741329305 80-81 22.029031057096866 27.427911427486052 27.623615343611647 22.919442171805443 82-83 22.17609579334551 27.323691519586546 27.50153733700626 22.998675350061685 84-85 22.15738615555045 27.156366319164054 27.448744691353532 23.237502833931966 86-87 22.137821172706737 27.058460761924458 27.681446390806734 23.122271674562068 88-89 22.087193438719705 26.947449681423002 27.61641096232834 23.348945917528955 90-91 21.969810966129362 27.094759674220043 27.710262586040574 23.22516677361002 92-93 22.13528338182295 26.869750591239722 27.633642165571388 23.361323861365943 94-95 21.911473256302184 26.771184119362584 27.62835866022287 23.688983964112367 96-97 22.016864833387547 26.690445156687055 27.544529696194907 23.748160313730484 98-99 21.844933308766272 26.6284209065701 27.485178251477244 24.041467533186385 100-101 21.871254394732937 26.284213613165686 28.110140201150674 23.734391790950703 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 564.0 1 390.0 2 297.0 3 410.5 4 366.5 5 330.5 6 646.5 7 1459.0 8 2368.5 9 2865.5 10 2709.0 11 2473.5 12 2750.5 13 3054.0 14 3302.0 15 3537.5 16 3782.0 17 4046.0 18 4273.0 19 4585.5 20 4988.0 21 5664.5 22 6632.5 23 7833.0 24 9357.5 25 11695.0 26 14637.0 27 17974.5 28 21405.5 29 25138.5 30 29701.0 31 34926.5 32 40765.5 33 45558.0 34 49729.0 35 54411.5 36 60068.0 37 66118.0 38 70642.0 39 73407.5 40 75625.0 41 80940.0 42 85928.0 43 89660.5 44 94771.5 45 99530.0 46 100869.0 47 101436.0 48 107442.0 49 108430.0 50 103592.0 51 99681.0 52 95201.0 53 93157.5 54 89514.5 55 80675.5 56 71495.5 57 63948.5 58 56893.0 59 49304.0 60 41448.0 61 33979.5 62 27436.0 63 21358.0 64 16374.5 65 12876.5 66 10084.0 67 8275.0 68 6269.0 69 4240.0 70 3052.5 71 2284.0 72 1792.0 73 1530.0 74 1244.5 75 1005.0 76 686.0 77 373.0 78 240.0 79 160.0 80 98.0 81 68.5 82 51.0 83 28.0 84 15.0 85 8.5 86 4.5 87 4.5 88 5.5 89 4.0 90 2.5 91 2.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2808909957925114 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0023884643993694454 16-17 6.255501998348547E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.8956066661662263E-5 26-27 0.0012511003996697094 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0033173116657908962 36-37 0.020055518528038677 38-39 0.00644506266496517 40-41 0.017193152462127673 42-43 0.004928577332032189 44-45 4.1703346655656983E-4 46-47 9.478033330831133E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.68681999849868E-4 60-61 0.0019524748661512133 62-63 0.0014975292662713188 64-65 1.8956066661662263E-5 66-67 0.0 68-69 5.307698665265434E-4 70-71 1.8956066661662263E-5 72-73 0.0 74-75 0.0 76-77 0.23090384800570807 78-79 0.0 80-81 7.582426664664906E-4 82-83 0.0 84-85 0.0 86-87 0.10257127670625452 88-89 0.3544594905064227 90-91 0.3540803691731894 92-93 0.0 94-95 1.8956066661662263E-5 96-97 0.0 98-99 0.0 100-101 1.3269246663163585E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2637678.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.06951167630384 #Duplication Level Percentage of deduplicated Percentage of total 1 69.80777432800349 33.556256231567474 2 15.49467594775981 14.896430127827687 3 5.9523050399491995 8.583731898562807 4 2.890411617343096 5.557626999567929 5 1.6174128772597753 3.887412359442148 6 0.9895233009351122 2.8539541120965057 7 0.6719150147383707 2.2609038652514983 8 0.45291470931949585 1.7417111126402613 9 0.33051875141878967 1.429908748250659 >10 1.6136857917084881 13.849119874855251 >50 0.11042477286564453 3.68857076951217 >100 0.06409125179276817 5.391222615843547 >500 0.0036353355941007464 1.1145934763367662 >1k 4.741742079222154E-4 0.30706404462061826 >5k 2.370871039611077E-4 0.881493763624499 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9570 0.3628191159042158 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8319 0.31539103711673677 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5315 0.20150298861346988 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.791213332332453E-5 0.0 10-11 0.0 0.0 0.0 3.791213332332453E-5 0.0 12-13 0.0 0.0 0.0 7.582426664664905E-5 0.0 14-15 0.0 0.0 0.0 7.582426664664905E-5 0.0 16-17 0.0 0.0 0.0 7.582426664664905E-5 0.0 18-19 0.0 0.0 0.0 1.1373639996997359E-4 0.0 20-21 0.0 0.0 0.0 1.8956066661662265E-4 0.0 22-23 0.0 0.0 0.0 3.791213332332453E-4 0.0 24-25 0.0 0.0 0.0 5.497259331882057E-4 0.0 26-27 0.0 0.0 0.0 9.667593997447755E-4 0.0 28-29 0.0 0.0 0.0 0.002293684066061134 0.0 30-31 0.0 0.0 0.0 0.006956876464830051 0.0 32-33 0.0 0.0 0.0 0.01641595372899952 0.0 34-35 0.0 0.0 0.0 0.03080360832520118 0.0 36-37 0.0 0.0 0.0 0.05603413305187366 0.0 38-39 0.0 0.0 0.0 0.09515945464154457 0.0 40-41 0.0 0.0 0.0 0.13695758163050986 0.0 42-43 0.0 0.0 0.0 0.17991202868583656 0.0 44-45 0.0 0.0 0.0 0.23696978933743995 0.0 46-47 0.0 0.0 0.0 0.3063110811858005 0.0 48-49 0.0 0.0 0.0 0.3823628206323896 0.0 50-51 0.0 0.0 0.0 0.46645193234352333 0.0 52-53 0.0 0.0 0.0 0.5516404959210336 0.0 54-55 0.0 1.8956066661662263E-5 0.0 0.6509134170281589 0.0 56-57 0.0 3.791213332332453E-5 0.0 0.7713602645963609 0.0 58-59 0.0 3.791213332332453E-5 0.0 0.9028774550949736 0.0 60-61 0.0 3.791213332332453E-5 0.0 1.0524029089221656 0.0 62-63 0.0 3.791213332332453E-5 0.0 1.209643481880654 0.0 64-65 0.0 3.791213332332453E-5 0.0 1.376286263903327 0.0 66-67 0.0 3.791213332332453E-5 0.0 1.5694296271190038 0.0 68-69 0.0 3.791213332332453E-5 0.0 1.7859458205285104 0.0 70-71 0.0 3.791213332332453E-5 0.0 2.0329054569966463 0.0 72-73 0.0 3.791213332332453E-5 0.0 2.2815332273310087 0.0 74-75 0.0 3.791213332332453E-5 0.0 2.5468612923942953 0.0 76-77 0.0 3.791213332332453E-5 0.0 2.8392017524504505 0.0 78-79 0.0 3.791213332332453E-5 0.0 3.1707054462296003 0.0 80-81 0.0 3.791213332332453E-5 0.0 3.532235549600823 0.0 82-83 0.0 3.791213332332453E-5 0.0 3.9113947949673915 0.0 84-85 0.0 3.791213332332453E-5 0.0 4.303273561063936 0.0 86-87 0.0 3.791213332332453E-5 0.0 4.724287801619455 0.0 88-89 0.0 3.791213332332453E-5 0.0 5.172674602434414 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7435 0.0 44.994526 1 GTATCAA 11665 0.0 35.77669 1 TATCAAC 14860 0.0 28.067402 2 ATCAACG 14805 0.0 27.979374 3 TCAACGC 15065 0.0 27.559484 4 CAACGCA 15265 0.0 27.198404 5 AACGCAG 15770 0.0 26.357521 6 GTACATG 12735 0.0 24.550014 1 TACATGG 13095 0.0 23.44396 2 ACGCAGA 17720 0.0 23.376673 7 CGCAGAG 17940 0.0 23.037104 8 GAGTACT 9780 0.0 22.779995 12-13 ACATGGG 13415 0.0 21.894354 3 GTACTTT 10410 0.0 21.196253 14-15 GCAGAGT 19615 0.0 20.925528 9 GGACCGA 275 1.2769306E-9 20.705261 6 CATGGGG 6440 0.0 20.040808 4 AGTACTT 10320 0.0 19.771774 12-13 CAGAGTA 18985 0.0 19.133188 10-11 AGAGTAC 18250 0.0 18.824717 10-11 >>END_MODULE