FastQCFastQC Report
Fri 27 May 2016
SRR939351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880610
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64750.1668552109075635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63050.16247445633547303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48910.12603688595349702No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG42950.11067847580663866No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG40400.10410734394850293No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG139150.026.975791
TACATGG144000.025.6492272
GTATCAA160900.025.5707781
ACATGGG145000.024.654873
CATGGGG103700.022.0377674
ATCAACG188700.021.7593213
TCAACGC191500.021.4659274
TATCAAC193450.021.4211122
CAACGCA192100.021.37425
GGTATCA123900.021.2953281
AACGCAG196150.020.9443366
ATGGGGA60200.020.0049085
AGTACTT81050.019.9632612-13
GAGTACT79100.019.88636412-13
AGAGTAC158350.018.80545210-11
GTACTTT83050.018.74460814-15
ACGCAGA218450.018.7195137
ACTTGAC19700.018.5213038
CGCAGAG220300.018.4767558
TGCACCG17550.018.1007675
Produced by FastQC (version 0.11.5)