##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939351_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3880610 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.926165731676207 33.0 31.0 34.0 30.0 34.0 2 31.988470369349148 34.0 31.0 34.0 30.0 34.0 3 32.05137929346159 34.0 31.0 34.0 30.0 34.0 4 35.5325057143078 37.0 35.0 37.0 33.0 37.0 5 35.55416390722077 37.0 35.0 37.0 33.0 37.0 6 35.63728640600318 37.0 35.0 37.0 33.0 37.0 7 35.56466199901562 37.0 35.0 37.0 33.0 37.0 8 35.54857122978088 37.0 35.0 37.0 33.0 37.0 9 37.17964160273771 39.0 37.0 39.0 34.0 39.0 10-11 37.07197515339084 39.0 37.0 39.0 33.0 39.0 12-13 36.97257892444745 39.0 37.0 39.0 33.0 39.0 14-15 38.23950061974793 40.0 38.0 41.0 33.0 41.0 16-17 38.15740721175279 40.0 38.0 41.0 33.0 41.0 18-19 38.0382760699993 40.0 38.0 41.0 33.0 41.0 20-21 37.99603954017539 40.0 38.0 41.0 33.0 41.0 22-23 38.00231535763707 40.0 38.0 41.0 33.0 41.0 24-25 37.91236635477412 40.0 38.0 41.0 33.0 41.0 26-27 37.83243330301164 40.0 38.0 41.0 32.5 41.0 28-29 37.68749900144565 40.0 38.0 41.0 32.0 41.0 30-31 37.52180610780264 40.0 37.5 41.0 32.0 41.0 32-33 37.330070272457164 40.0 37.0 41.0 31.5 41.0 34-35 37.0471704190836 40.0 37.0 41.0 30.5 41.0 36-37 36.81478324284069 40.0 36.0 41.0 30.0 41.0 38-39 36.23509293641979 39.5 35.5 40.5 29.0 41.0 40-41 36.20992369756301 39.0 35.0 40.0 30.0 41.0 42-43 36.42860130237256 39.0 36.0 41.0 30.0 41.0 44-45 36.36563736113652 40.0 36.0 41.0 30.0 41.0 46-47 36.12655175346144 39.0 35.0 41.0 29.0 41.0 48-49 35.90225338284445 39.0 35.0 41.0 28.0 41.0 50-51 34.75347883451313 38.0 34.0 40.0 26.0 40.5 52-53 34.8408497633104 38.0 34.5 39.5 26.5 40.5 54-55 35.305498877753756 39.0 35.0 40.0 27.0 41.0 56-57 35.187577855027946 38.5 35.0 40.0 26.5 41.0 58-59 34.95163878874713 38.0 34.5 40.0 26.0 41.0 60-61 34.624347976220236 38.0 34.0 40.0 26.0 41.0 62-63 34.217034306462125 37.0 34.0 40.0 25.5 41.0 64-65 33.803905056163856 36.5 33.5 39.5 24.5 41.0 66-67 33.42299522497751 36.0 33.0 39.0 23.5 41.0 68-69 33.00833618941352 35.5 33.0 39.0 22.5 40.0 70-71 32.581339789363014 35.0 32.5 38.0 22.0 40.0 72-73 32.14343569696517 35.0 32.0 37.0 20.5 39.5 74-75 31.69060907950039 35.0 31.5 37.0 20.0 39.0 76-77 31.240745011737843 35.0 31.0 36.0 18.5 39.0 78-79 30.865311123766624 35.0 31.0 36.0 17.5 37.5 80-81 30.487089916276048 34.0 31.0 35.0 13.0 37.0 82-83 30.093700088388168 34.0 30.0 35.0 7.5 37.0 84-85 29.709430862673653 34.0 30.0 35.0 6.0 36.0 86-87 29.301978554917913 34.0 29.5 35.0 2.0 36.0 88-89 28.90084213564362 34.0 29.0 35.0 2.0 35.0 90-91 28.51393041300208 34.0 29.0 35.0 2.0 35.0 92-93 28.079287792383155 34.0 28.5 35.0 2.0 35.0 94-95 27.6017781998191 34.0 27.0 35.0 2.0 35.0 96-97 26.993440335411186 33.0 26.0 35.0 2.0 35.0 98-99 26.187832454176018 33.0 24.5 35.0 2.0 35.0 100-101 23.439623152030222 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29059981519691647 1101 2 0.34158114105684945 1101 3 0.39741464794128234 1101 4 0.252699561934719 1101 5 0.243376319577024 1101 6 0.30304582778310873 1101 7 0.20220412451317316 1101 8 0.13070143323255934 1101 9 0.252421412978471 1101 10-11 0.4222508619394745 1101 12-13 0.4124092592868678 1101 14-15 0.4592041557113973 1101 16-17 0.4652462351484914 1101 18-19 0.5288508614671343 1101 20-21 0.5169642408791546 1101 22-23 0.5343408211140215 1101 24-25 0.541498328994301 1101 26-27 0.4839488367309812 1101 28-29 0.5778599863208385 1101 30-31 0.5585224252393246 1101 32-33 0.5793935710828251 1101 34-35 0.44898140604328773 1101 36-37 0.3556397769895838 1101 38-39 0.772993077004152 1101 40-41 0.6195388899355052 1101 42-43 0.5881835688078851 1101 44-45 0.48585179732493344 1101 46-47 0.6114156115874252 1101 48-49 0.6171643853711899 1101 50-51 0.5585162581391288 1101 52-53 0.6111548018274817 1101 54-55 0.6593693711495874 1101 56-57 0.6606341344217199 1101 58-59 0.6686297414147617 1101 60-61 0.6993981985284989 1101 62-63 0.7173091856446305 1101 64-65 0.5146276097891942 1101 66-67 0.47135214297978933 1101 68-69 0.5191728062315946 1101 70-71 0.5622642219639218 1101 72-73 0.5187682846814639 1101 74-75 0.4869375949978796 1101 76-77 0.5076181765391539 1101 78-79 0.4430068588191851 1101 80-81 0.38107304558648636 1101 82-83 0.38291878028997317 1101 84-85 0.4699040073413876 1101 86-87 0.4796274303812105 1101 88-89 0.471750724307622 1101 90-91 0.4970976975209602 1101 92-93 0.5686229927151167 1101 94-95 0.5940508582752493 1101 96-97 0.5130017124872062 1101 98-99 0.32636203237469985 1101 100-101 -0.09166675226518706 1102 1 0.2592472042446836 1102 2 0.3269087046719221 1102 3 0.39734621584555896 1102 4 0.3624078150834933 1102 5 0.2869863089968234 1102 6 0.33125889319585866 1102 7 0.34143160351047186 1102 8 0.3229081481468299 1102 9 0.40426449758790994 1102 10-11 0.5100336203836946 1102 12-13 0.3665927810762426 1102 14-15 0.5382972281220759 1102 16-17 0.5638916961963503 1102 18-19 0.5846567892337831 1102 20-21 0.6734248555302926 1102 22-23 0.6974457459636838 1102 24-25 0.5560754067414919 1102 26-27 0.4747021729444114 1102 28-29 0.43514380933044094 1102 30-31 0.5013655586438261 1102 32-33 0.38493722540434305 1102 34-35 0.3622501216264027 1102 36-37 0.3708270905121296 1102 38-39 0.6907336812774076 1102 40-41 0.5775699319242449 1102 42-43 0.3496273491634625 1102 44-45 0.4514245025587442 1102 46-47 0.47320063007599344 1102 48-49 0.20640325980007646 1102 50-51 0.19879967412652633 1102 52-53 0.28367479292536046 1102 54-55 0.31742430486566775 1102 56-57 0.2924867162159259 1102 58-59 0.32636289551551556 1102 60-61 0.3455517494401903 1102 62-63 0.47495587728008815 1102 64-65 0.44608007209500755 1102 66-67 0.4255431030404324 1102 68-69 0.3787949241990063 1102 70-71 0.2974306726231859 1102 72-73 0.3001140524241208 1102 74-75 0.15883857316030614 1102 76-77 0.2002763945197792 1102 78-79 0.23024924173187244 1102 80-81 0.1845713240729836 1102 82-83 0.14196044532022256 1102 84-85 -0.02398589910301041 1102 86-87 -0.1332178392948009 1102 88-89 -0.05421970231591544 1102 90-91 -0.00696969797727931 1102 92-93 0.02444992615984276 1102 94-95 -0.006219269984349296 1102 96-97 -0.19225901974811777 1102 98-99 -0.16398948842448746 1102 100-101 -0.37896655863211137 1103 1 0.2756891977087399 1103 2 0.30355210079441264 1103 3 0.36588164031496717 1103 4 0.3135339350071149 1103 5 0.22957874859438476 1103 6 0.3532488689614439 1103 7 0.3016995590072753 1103 8 0.30885517208749036 1103 9 0.4051675507567438 1103 10-11 0.3891220300921532 1103 12-13 0.4109089391874292 1103 14-15 0.48249095195992453 1103 16-17 0.4260441297353026 1103 18-19 0.49138126507281044 1103 20-21 0.5550623536943462 1103 22-23 0.485884599969701 1103 24-25 0.6033371016816389 1103 26-27 0.5382102189530755 1103 28-29 0.46632840395480457 1103 30-31 0.36559489972483306 1103 32-33 0.41807250667040563 1103 34-35 0.3152643914729225 1103 36-37 0.18226912987469035 1103 38-39 0.6157303536354846 1103 40-41 0.4424189028796732 1103 42-43 0.3790999293940658 1103 44-45 0.28995239967264297 1103 46-47 0.33794116580339306 1103 48-49 0.23666472065782784 1103 50-51 0.21897895370353382 1103 52-53 0.26093724519091666 1103 54-55 0.40713466232325857 1103 56-57 0.34557465316973435 1103 58-59 0.3425558895670662 1103 60-61 0.47234007010553825 1103 62-63 0.43345501897795913 1103 64-65 0.38598458024658555 1103 66-67 0.4180656621577157 1103 68-69 0.29664864106027977 1103 70-71 0.24465197687522533 1103 72-73 0.24737609160309404 1103 74-75 0.19368357389468116 1103 76-77 0.24861727815788015 1103 78-79 0.29382211938480296 1103 80-81 0.2951586184476618 1103 82-83 0.3755224679757241 1103 84-85 0.4441605355007958 1103 86-87 0.28980232851014875 1103 88-89 0.20913848506016208 1103 90-91 0.2128606381834004 1103 92-93 0.27614702353811893 1103 94-95 0.15003876225476276 1103 96-97 0.1682026274575783 1103 98-99 0.2620662920337615 1103 100-101 -0.008685499110921313 1104 1 0.21023926773674972 1104 2 0.29053564421177924 1104 3 0.16573283079115697 1104 4 0.15277588178582846 1104 5 0.24526502310418863 1104 6 0.13257309865332445 1104 7 0.2686988587271628 1104 8 0.2590214886141027 1104 9 0.3629143994962405 1104 10-11 0.299109775190189 1104 12-13 0.3114691632770601 1104 14-15 0.3695489001391934 1104 16-17 0.40025193785855606 1104 18-19 0.3461825605910107 1104 20-21 0.4169452318456095 1104 22-23 0.4184301181769854 1104 24-25 0.4387187543427089 1104 26-27 0.4435279710539177 1104 28-29 0.481436947372174 1104 30-31 0.45070957059317607 1104 32-33 0.39687152627825384 1104 34-35 0.33881568699112563 1104 36-37 0.3073216508830896 1104 38-39 0.6950764670808667 1104 40-41 0.5427646661849934 1104 42-43 0.3597809518030246 1104 44-45 0.3703182960311793 1104 46-47 0.3946988688846318 1104 48-49 0.3727160161760352 1104 50-51 0.33777272121053414 1104 52-53 0.34957957712368426 1104 54-55 0.4552570362728332 1104 56-57 0.513357391264968 1104 58-59 0.27110105763429715 1104 60-61 0.3528066317301892 1104 62-63 0.22162466183510077 1104 64-65 0.11602222030261089 1104 66-67 0.23618103330617402 1104 68-69 0.24947164526196275 1104 70-71 0.3567755763150018 1104 72-73 0.188758269474242 1104 74-75 0.23104351787226918 1104 76-77 0.2298716549085782 1104 78-79 0.15632211938480722 1104 80-81 0.11063918567455389 1104 82-83 0.16023447777964606 1104 84-85 0.18231704810583338 1104 86-87 0.1567578607230331 1104 88-89 0.1306502147520341 1104 90-91 0.0775446367828394 1104 92-93 4.02975919072901E-4 1104 94-95 0.0928783981091037 1104 96-97 0.20911649300379764 1104 98-99 0.1665699685043478 1104 100-101 -0.14118287306050448 1105 1 0.20436022557547417 1105 2 0.280139444184492 1105 3 0.3272683404104839 1105 4 0.2920163989095812 1105 5 0.17848493714430447 1105 6 0.2747915118065478 1105 7 0.29868077986238006 1105 8 0.2462486000890678 1105 9 0.2706303225416775 1105 10-11 0.33743355742514325 1105 12-13 0.30532874507539276 1105 14-15 0.34450839324875915 1105 16-17 0.382486468490292 1105 18-19 0.33606521852524907 1105 20-21 0.3760520335920958 1105 22-23 0.3195604736142954 1105 24-25 0.3331049257167038 1105 26-27 0.2604138013172559 1105 28-29 0.2965093481089838 1105 30-31 0.3668505004608278 1105 32-33 0.4208776151034712 1105 34-35 0.3762359523752181 1105 36-37 0.34137813880777657 1105 38-39 0.7377732879295564 1105 40-41 0.5518582498935487 1105 42-43 0.4272671368214418 1105 44-45 0.3604175592321681 1105 46-47 0.35414976600565495 1105 48-49 0.3503822223433133 1105 50-51 0.30018190049829485 1105 52-53 0.2697464655122772 1105 54-55 0.4541863752003792 1105 56-57 0.4541333327303789 1105 58-59 0.5358434338111238 1105 60-61 0.5862938912471733 1105 62-63 0.49424328306583476 1105 64-65 0.5105768150631533 1105 66-67 0.4973997364034588 1105 68-69 0.4235519256549267 1105 70-71 0.3975446955672908 1105 72-73 0.360992288439995 1105 74-75 0.3134085233300432 1105 76-77 0.32046936605607357 1105 78-79 0.41953546704478484 1105 80-81 0.3891065754958092 1105 82-83 0.32598185122759205 1105 84-85 0.4539251290172821 1105 86-87 0.4765721259706801 1105 88-89 0.3921887090895986 1105 90-91 0.3745322032994203 1105 92-93 0.40748358036715615 1105 94-95 0.32733611744325586 1105 96-97 0.42260912501362213 1105 98-99 0.3992615874155234 1105 100-101 0.22927054827938065 1106 1 0.2253944867525668 1106 2 0.21003789001828466 1106 3 0.25292428082693164 1106 4 0.27729144343619794 1106 5 0.2940929307280058 1106 6 0.2916204195850369 1106 7 0.1866027064098077 1106 8 0.27347911640645606 1106 9 0.22331268234861312 1106 10-11 0.3799528469765079 1106 12-13 0.42021315127292524 1106 14-15 0.48835678431122886 1106 16-17 0.43135859379304975 1106 18-19 0.48103032273427004 1106 20-21 0.4603812164230803 1106 22-23 0.4129490087883241 1106 24-25 0.3912045441511154 1106 26-27 0.40824048794715395 1106 28-29 0.5280381775971534 1106 30-31 0.46526085646645754 1106 32-33 0.5041631929449224 1106 34-35 0.2770110423452934 1106 36-37 0.19742817763403053 1106 38-39 0.7162566085865478 1106 40-41 0.4807796614151414 1106 42-43 0.4459101154885303 1106 44-45 0.3062402691610018 1106 46-47 0.2783857179982334 1106 48-49 0.2221314605674749 1106 50-51 0.09449349591439926 1106 52-53 0.18068374875895188 1106 54-55 0.2036360310259937 1106 56-57 0.374724799246529 1106 58-59 0.37333021933235955 1106 60-61 0.36737729513745165 1106 62-63 0.09780445553889905 1106 64-65 0.22272560491187932 1106 66-67 0.11867293449068939 1106 68-69 0.19298091666668427 1106 70-71 0.17402977974929712 1106 72-73 0.10502819355405535 1106 74-75 0.1328289837280643 1106 76-77 0.3307995406705615 1106 78-79 0.43197772595130246 1106 80-81 0.44562308741555157 1106 82-83 0.3294957465609478 1106 84-85 0.3674149791814365 1106 86-87 0.466822750618892 1106 88-89 0.49333380446504904 1106 90-91 0.4757571834876657 1106 92-93 0.4983663474112454 1106 94-95 0.5667969826547754 1106 96-97 0.7859836923042209 1106 98-99 0.8299446902628347 1106 100-101 0.3150743314072635 1107 1 0.05353190428018095 1107 2 -0.04539448662649548 1107 3 0.045577460336268416 1107 4 0.015276832645405136 1107 5 0.020777536416225928 1107 6 0.019739346941776148 1107 7 -0.05300546197875633 1107 8 -0.0023831211531302188 1107 9 0.0530711011545435 1107 10-11 0.16125035027006618 1107 12-13 0.1881212817921991 1107 14-15 0.15595018189790721 1107 16-17 0.18519174844732333 1107 18-19 0.21568659223562037 1107 20-21 0.13471774820039428 1107 22-23 0.017300021569653268 1107 24-25 0.14457700019696773 1107 26-27 0.20784126026736516 1107 28-29 0.16417361632090888 1107 30-31 0.14645875285393828 1107 32-33 0.15392357633150056 1107 34-35 0.07273332353456397 1107 36-37 -0.019899557088912445 1107 38-39 0.4821181527647269 1107 40-41 0.30336247160109053 1107 42-43 0.04971420146070926 1107 44-45 0.11270951769557058 1107 46-47 0.17488343072852786 1107 48-49 0.1835638976608962 1107 50-51 0.10198379301944982 1107 52-53 0.1702612483544783 1107 54-55 0.21465390604311096 1107 56-57 0.1855556835211658 1107 58-59 0.1727569795877386 1107 60-61 0.22364044049330545 1107 62-63 0.18509406317391353 1107 64-65 0.2526307894491211 1107 66-67 0.2712197332909625 1107 68-69 0.27587844200622413 1107 70-71 0.30853799454107644 1107 72-73 0.26527441506986094 1107 74-75 0.27030118180045903 1107 76-77 0.1827094953163062 1107 78-79 0.22365105974689214 1107 80-81 0.2612496375230222 1107 82-83 0.24411360451021125 1107 84-85 0.26075202832795696 1107 86-87 0.41925500814430094 1107 88-89 0.3394784704952265 1107 90-91 0.29788685114462155 1107 92-93 0.3335718105455747 1107 94-95 0.251912039520942 1107 96-97 0.3239632144399778 1107 98-99 0.5363416671198955 1107 100-101 0.23905683863887717 1108 1 0.04505815123728141 1108 2 0.055899157458490834 1108 3 0.12079034939331734 1108 4 0.03560274601169766 1108 5 0.12430266238897758 1108 6 0.056977166447843786 1108 7 0.11629153015696403 1108 8 0.19412202662666544 1108 9 0.21084502549113182 1108 10-11 0.2172038410103525 1108 12-13 0.21683205182454657 1108 14-15 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7126.0 9 8881.0 10 10001.0 11 12027.0 12 14581.0 13 18383.0 14 18248.0 15 17924.0 16 21745.0 17 26439.0 18 24243.0 19 20359.0 20 21744.0 21 23763.0 22 25312.0 23 28335.0 24 32413.0 25 37445.0 26 43312.0 27 50812.0 28 60338.0 29 72583.0 30 87357.0 31 107181.0 32 135061.0 33 176310.0 34 243090.0 35 355350.0 36 563620.0 37 842292.0 38 663252.0 39 75016.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15515775800421 17.623135652422473 13.805004537056156 24.416702052517167 2 15.832255952614524 19.31659297107181 39.1451138891241 25.706037187189562 3 19.658929441796296 22.557678089038326 28.88257028145627 28.900822187709107 4 13.699413263705468 15.453063549456347 34.890268719180725 35.957254467657464 5 15.341456397881755 35.87111525912734 32.88642877322994 15.900999569760963 6 34.563375492768 33.630299069967705 16.69453246470812 15.111792972556165 7 30.88336527575235 31.169066734370144 19.41554661787062 18.53202137200688 8 27.4283736884928 34.044777363435394 19.047114006776912 19.4797349412949 9 27.124404043792698 16.762927169151563 19.408088875699182 36.704579911356554 10-11 26.459247932813806 25.207686222216257 26.1824748849069 22.15059096006304 12-13 26.823849390034592 22.341468914397154 26.98847793671747 23.846203758850784 14-15 23.541319496948173 24.023477919360804 24.83500998352164 27.600192600169375 16-17 24.014118470758696 27.32990045637116 25.28122851952763 23.374752553342514 18-19 23.8787263536982 25.995539368441044 27.03773395994463 23.088000317916123 20-21 24.44480953882342 25.29290972806305 26.770793419060535 23.491487314052996 22-23 24.28305151858852 24.872588381485254 26.966142479023286 23.878217620902937 24-25 24.299800174355266 25.203746895434477 26.77229881337467 23.724154116835585 26-27 23.9486999982963 25.516208654906997 26.847083495314045 23.68800785148266 28-29 23.682912386382526 25.744353845311934 27.088364786043446 23.484368982262097 30-31 23.831718471346292 25.288004405354 26.78289009857457 24.09738702472514 32-33 23.772329711207842 25.372917421966285 26.86932379834923 23.98542906847664 34-35 24.506419172321074 25.434018697582513 26.24480843595781 23.814753694138602 36-37 24.496855850696402 25.116248603204422 26.53665636912893 23.850239176970245 38-39 23.857363057817754 25.338229697127108 26.766206393458468 24.03820085159667 40-41 23.949818833219165 25.333989455293693 26.769012548526582 23.94717916296056 42-43 24.154826091019384 25.223150554760448 26.358569672439863 24.263453681780305 44-45 24.06185118403312 25.344439938789243 26.358846573552764 24.234862303624872 46-47 24.187552178724673 25.712199952108826 26.0617019486529 24.038545920513602 48-49 24.443495417203838 25.493170175442458 26.04196912594893 24.021365281404773 50-51 24.051419233814116 25.69532645147795 26.16304115392322 24.090213160784714 52-53 24.378989850242995 25.678823091104963 25.864611567196732 24.07757549145531 54-55 23.93180701269141 25.34920891107703 26.097139763422067 24.621844312809486 56-57 24.049355804769533 25.839615758676203 26.13174546431726 23.979282972237005 58-59 24.334806617683682 25.219902728303556 26.177237853813022 24.268052800199744 60-61 24.229760009434518 25.26339324045712 26.66159250948883 23.845254240619536 62-63 23.863785374368092 25.55600243411457 26.38598838527099 24.19422380624634 64-65 23.705833986168017 25.50462869714563 26.23745612459037 24.55208119209598 66-67 23.808313699091215 25.64073220619838 26.074903554876627 24.47605053983378 68-69 23.502767320807756 25.42614202800828 26.555455948924067 24.5156347022599 70-71 23.69506767792578 25.624554000967347 26.256733355497346 24.423644965609526 72-73 23.909065053821973 25.377707878980726 26.241805795061346 24.471421272135956 74-75 23.752381726670603 25.864250577194092 25.947389311688436 24.43597838444687 76-77 23.908014289042477 25.5793663120292 25.879953923015197 24.632665475913125 78-79 23.956787004007218 25.611995385195748 26.10973683580498 24.321480774992057 80-81 23.934188203414045 26.01441088891736 26.01897899576706 24.032421911901537 82-83 24.016691043687217 25.743182037908564 25.908598921198738 24.331527997205477 84-85 23.86412612293129 25.532399827604728 25.896565425429337 24.706908624034646 86-87 24.03403474789289 25.355980367933633 26.10343656937652 24.506548314796955 88-89 23.88748457603695 25.398201326127523 25.99901913108579 24.715294966749735 90-91 23.858975927439896 25.5982279426567 26.121753855176255 24.421042274727146 92-93 23.982959727759575 25.131967599919992 26.18140342087155 24.703669251448883 94-95 23.8928841639518 25.09863901718823 26.237732589481993 24.770744229377986 96-97 23.936323500892122 25.113932742591103 26.304281012607756 24.64546274390902 98-99 23.91843595423463 25.175703223923882 25.946610868672803 24.959249953168676 100-101 24.06562245498643 24.506866003304214 26.454822304013568 24.97268923769579 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4082.0 1 2310.0 2 517.5 3 507.0 4 461.5 5 463.5 6 630.5 7 1027.5 8 1626.0 9 2041.5 10 2162.0 11 2336.0 12 2666.5 13 3024.0 14 3340.5 15 3573.5 16 3853.0 17 3984.0 18 3990.5 19 4129.0 20 4257.0 21 4546.0 22 5363.0 23 6481.0 24 7840.5 25 9381.0 26 11501.5 27 14208.0 28 16905.0 29 20370.5 30 24284.0 31 28758.5 32 33639.0 33 37338.0 34 41183.5 35 44610.0 36 50316.0 37 58996.5 38 66820.5 39 71498.0 40 76986.0 41 92154.5 42 107482.5 43 117497.5 44 127049.5 45 137979.5 46 146171.5 47 154446.5 48 174069.5 49 179288.5 50 170749.0 51 168232.0 52 167941.5 53 171707.5 54 172742.0 55 161899.5 56 143059.5 57 129074.0 58 117545.0 59 102502.0 60 87504.0 61 73627.5 62 61752.5 63 50962.0 64 41350.0 65 32873.5 66 25207.0 67 21801.5 68 17257.5 69 11457.5 70 8519.0 71 6452.5 72 5248.5 73 4210.0 74 3103.5 75 2491.0 76 1778.0 77 1092.0 78 696.0 79 473.5 80 321.0 81 217.5 82 163.0 83 106.0 84 71.0 85 64.5 86 50.0 87 40.0 88 37.0 89 29.0 90 20.0 91 16.0 92 12.0 93 13.0 94 11.0 95 6.5 96 4.5 97 3.0 98 3.0 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29276325113835194 2 0.18084785639371131 3 0.1814663158627123 4 0.2017981709061204 5 0.21501774205601698 6 0.11062693751755523 7 0.12590804023078847 8 0.13897299651343473 9 0.14263221503835738 10-11 0.1301857182247121 12-13 0.11883441005408944 14-15 0.13461801108588597 16-17 0.11002136262082507 18-19 0.13848338276714228 20-21 0.1654765616745821 22-23 0.16883943503727508 24-25 0.1928433931778767 26-27 0.1724471152731143 28-29 0.1712617346241957 30-31 0.16063196250074085 32-33 0.1699603928248394 34-35 0.3514782469766351 36-37 0.468946892370014 38-39 0.4361814250852314 40-41 0.42495896263731736 42-43 0.4486665756156893 44-45 0.45259637015829984 46-47 0.4557273212201175 48-49 0.4683670866178256 50-51 0.46106153414025114 52-53 0.46778728086563703 54-55 0.45058637688404657 56-57 0.41383957676757005 58-59 0.40360922638451174 60-61 0.3596857195131693 62-63 0.46421825434661046 64-65 0.46301998912542097 66-67 0.4718330365586854 68-69 0.48322299844612054 70-71 0.4765745591543597 72-73 0.46901131523136824 74-75 0.4739203372665638 76-77 0.4512177209253184 78-79 0.4369802685660244 80-81 0.43459662269591637 82-83 0.44684985092549884 84-85 0.4482542693030219 86-87 0.4494267653796697 88-89 0.4563328961168476 90-91 0.4749897567650447 92-93 0.47885512844630096 94-95 0.477966092959612 96-97 0.47577571567356675 98-99 0.47272207204537436 100-101 0.4551861691847416 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3880610.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.99229736629369 #Duplication Level Percentage of deduplicated Percentage of total 1 65.88114486614666 23.05332611988077 2 14.8157082449706 10.368713372005207 3 6.468819434324104 6.7907655976418635 4 3.5660223479783695 4.991332576612318 5 2.16966374595367 3.7960759491638747 6 1.4605454230199044 3.066470385557503 7 1.0040370347952612 2.4593493741829278 8 0.7468633406678996 2.0907571286907722 9 0.5250808118456489 1.6536405518494068 >10 2.9528880697745086 18.644919959962085 >50 0.22943700196829064 5.578247154144186 >100 0.16087878289789656 10.917970078727107 >500 0.014404783807781424 3.4365019746603735 >1k 0.004358370477468533 2.821578916231798 >5k 1.4774137211757737E-4 0.33035086068967695 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6475 0.1668552109075635 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6305 0.16247445633547303 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4891 0.12603688595349702 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 4295 0.11067847580663866 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 4040 0.10410734394850293 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.576914454170865E-5 0.0 16-17 0.0 0.0 0.0 3.865371681256297E-5 1.2884572270854325E-5 18-19 0.0 0.0 0.0 6.442286135427161E-5 2.576914454170865E-5 20-21 0.0 0.0 0.0 1.030765781668346E-4 2.576914454170865E-5 22-23 0.0 0.0 0.0 1.8038401179196054E-4 2.576914454170865E-5 24-25 0.0 0.0 0.0 3.478834513130668E-4 2.576914454170865E-5 26-27 0.0 0.0 0.0 7.086514748969879E-4 2.576914454170865E-5 28-29 0.0 0.0 0.0 0.002576914454170865 2.576914454170865E-5 30-31 0.0 0.0 0.0 0.007898242802033701 2.576914454170865E-5 32-33 0.0 0.0 0.0 0.01894032123815586 2.576914454170865E-5 34-35 0.0 0.0 0.0 0.03602526406930869 2.576914454170865E-5 36-37 0.0 0.0 0.0 0.0643455539206465 2.576914454170865E-5 38-39 0.0 0.0 0.0 0.10647810524634013 2.576914454170865E-5 40-41 0.0 0.0 0.0 0.15632851536227552 2.576914454170865E-5 42-43 0.0 0.0 0.0 0.20745449813302547 2.576914454170865E-5 44-45 0.0 0.0 0.0 0.2700477502248358 2.576914454170865E-5 46-47 0.0 0.0 0.0 0.34899152452836024 2.576914454170865E-5 48-49 0.0 0.0 0.0 0.43480277585225 2.576914454170865E-5 50-51 0.0 0.0 0.0 0.5313855295945741 2.576914454170865E-5 52-53 0.0 0.0 0.0 0.6253784843104564 2.576914454170865E-5 54-55 0.0 0.0 0.0 0.7337119679637996 2.576914454170865E-5 56-57 0.0 0.0 0.0 0.8644259536516166 2.576914454170865E-5 58-59 0.0 0.0 0.0 1.0074704750026413 2.576914454170865E-5 60-61 0.0 0.0 0.0 1.1651776395978983 2.576914454170865E-5 62-63 0.0 0.0 0.0 1.3251009506237421 2.576914454170865E-5 64-65 0.0 0.0 0.0 1.5024957416488645 2.576914454170865E-5 66-67 0.0 0.0 0.0 1.7054019857702785 2.576914454170865E-5 68-69 0.0 0.0 0.0 1.9300573878848946 2.576914454170865E-5 70-71 0.0 0.0 0.0 2.182852695839056 2.576914454170865E-5 72-73 0.0 0.0 0.0 2.4478496937337173 2.576914454170865E-5 74-75 0.0 0.0 0.0 2.727676834312131 2.576914454170865E-5 76-77 0.0 0.0 0.0 3.0372802214084897 2.576914454170865E-5 78-79 0.0 0.0 0.0 3.381388493046196 2.576914454170865E-5 80-81 0.0 0.0 0.0 3.7534820556561983 2.576914454170865E-5 82-83 0.0 0.0 0.0 4.1437042114512925 2.576914454170865E-5 84-85 0.0 0.0 0.0 4.549374969399141 2.576914454170865E-5 86-87 0.0 0.0 0.0 4.98145910050224 2.576914454170865E-5 88-89 0.0 0.0 0.0 5.448151192724855 2.576914454170865E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13915 0.0 26.97579 1 TACATGG 14400 0.0 25.649227 2 GTATCAA 16090 0.0 25.570778 1 ACATGGG 14500 0.0 24.65487 3 CATGGGG 10370 0.0 22.037767 4 ATCAACG 18870 0.0 21.759321 3 TCAACGC 19150 0.0 21.465927 4 TATCAAC 19345 0.0 21.421112 2 CAACGCA 19210 0.0 21.3742 5 GGTATCA 12390 0.0 21.295328 1 AACGCAG 19615 0.0 20.944336 6 ATGGGGA 6020 0.0 20.004908 5 AGTACTT 8105 0.0 19.96326 12-13 GAGTACT 7910 0.0 19.886364 12-13 AGAGTAC 15835 0.0 18.805452 10-11 GTACTTT 8305 0.0 18.744608 14-15 ACGCAGA 21845 0.0 18.719513 7 ACTTGAC 1970 0.0 18.521303 8 CGCAGAG 22030 0.0 18.476755 8 TGCACCG 1755 0.0 18.100767 5 >>END_MODULE