FastQCFastQC Report
Fri 27 May 2016
SRR939351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939351_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880610
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64530.16628828972764592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61520.1585317772205916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50350.12974764276750306No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG44560.11482730807785373No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG41600.10719964129350798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG139650.025.4963261
TACATGG143550.024.6951562
ACATGGG147200.023.438023
GTATCAA168800.022.7289621
GGTATCA135600.021.132641
CATGGGG104450.020.809014
GAGTACT78400.020.27895212-13
ATCAACG198950.019.2019943
TCAACGC198950.019.178144
CAACGCA200050.019.0489645
AGTACTT81100.018.81381412-13
TATCAAC203600.018.786752
GTACTTT83900.018.69503414-15
AACGCAG205250.018.6126026
AGAGTAC162400.017.76759710-11
ATGGGGA62600.017.7392755
AGTACAT109450.017.7338052
GATGTGC28000.017.4571598
GTATAAC6500.016.8434661
GAGTACA126150.016.6405741
Produced by FastQC (version 0.11.5)