##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939351_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3880610 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33762217795656 34.0 31.0 34.0 31.0 34.0 2 32.55152102375657 34.0 31.0 34.0 31.0 34.0 3 32.645727862372155 34.0 31.0 34.0 31.0 34.0 4 36.15894331045892 37.0 35.0 37.0 35.0 37.0 5 36.0917933005378 37.0 35.0 37.0 35.0 37.0 6 36.1173240804925 37.0 36.0 37.0 35.0 37.0 7 36.05567578293103 37.0 35.0 37.0 35.0 37.0 8 36.02599410917356 37.0 35.0 37.0 35.0 37.0 9 37.74328082440647 39.0 38.0 39.0 35.0 39.0 10-11 37.66204643084464 39.0 37.0 39.0 35.0 39.0 12-13 37.5843469196853 39.0 37.0 39.0 35.0 39.0 14-15 38.91550374812208 40.0 38.0 41.0 35.0 41.0 16-17 38.91108794751341 40.0 38.0 41.0 35.0 41.0 18-19 38.97893320895426 40.0 38.0 41.0 35.0 41.0 20-21 38.944574048925304 40.0 38.0 41.0 35.0 41.0 22-23 38.86991503913045 40.0 38.0 41.0 35.0 41.0 24-25 38.79611646107184 40.0 38.0 41.0 34.5 41.0 26-27 38.64869105630301 40.0 38.0 41.0 34.0 41.0 28-29 38.48441817136997 40.0 38.0 41.0 34.0 41.0 30-31 38.311061276448804 40.0 38.0 41.0 34.0 41.0 32-33 38.31779307892316 40.0 38.0 41.0 34.0 41.0 34-35 38.239899268413986 40.0 38.0 41.0 34.0 41.0 36-37 38.10124439198992 40.0 38.0 41.0 33.0 41.0 38-39 37.97990187109758 40.0 38.0 41.0 33.0 41.0 40-41 37.80897036806069 40.0 37.0 41.0 33.0 41.0 42-43 37.63960575785765 40.0 37.0 41.0 33.0 41.0 44-45 37.42382615104326 40.0 37.0 41.0 32.0 41.0 46-47 37.156896209616534 40.0 36.0 41.0 31.5 41.0 48-49 36.88452820561716 39.5 36.0 41.0 31.0 41.0 50-51 36.6361636443755 39.0 35.5 41.0 30.5 41.0 52-53 36.36572420315363 39.0 35.0 40.5 30.0 41.0 54-55 36.09153290333221 39.0 35.0 40.0 30.0 41.0 56-57 35.75701655152154 38.0 35.0 40.0 29.0 41.0 58-59 35.41431140980413 38.0 34.0 40.0 28.0 41.0 60-61 35.04637582751165 37.5 34.0 40.0 28.0 41.0 62-63 34.67667286844079 37.0 34.0 40.0 28.0 41.0 64-65 34.34459646808105 36.0 34.0 39.0 27.0 41.0 66-67 33.915675241779 36.0 33.0 39.0 26.0 40.0 68-69 33.4403214958473 35.0 33.0 38.5 26.0 40.0 70-71 32.947700103849655 35.0 32.0 37.5 25.5 40.0 72-73 32.462374858591815 35.0 32.0 37.0 24.5 39.0 74-75 31.955222890215715 35.0 31.0 36.5 23.5 39.0 76-77 30.02388297200698 33.0 29.5 35.0 20.0 36.5 78-79 31.003805716111643 34.0 30.5 35.0 21.0 37.0 80-81 31.035809834020938 34.0 31.0 35.0 21.5 37.0 82-83 30.721683317828898 34.0 31.0 35.0 20.0 36.5 84-85 30.31100625932521 34.0 30.5 35.0 18.5 36.0 86-87 29.733549493507464 34.0 30.0 35.0 14.5 35.5 88-89 29.17806195417731 34.0 29.0 35.0 7.0 35.0 90-91 28.716431050788408 33.5 29.0 35.0 3.5 35.0 92-93 28.370572796544874 33.0 29.0 35.0 2.0 35.0 94-95 27.855378535848747 33.0 27.5 35.0 2.0 35.0 96-97 27.12748073112217 33.0 26.5 34.5 2.0 35.0 98-99 26.128878191830665 32.5 24.5 34.0 2.0 35.0 100-101 21.826224743017207 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2860483634158868 1101 2 0.2787909022847259 1101 3 0.24788121016874243 1101 4 0.14241504563470642 1101 5 0.19874944392186222 1101 6 0.21676379510463306 1101 7 0.25507267857090454 1101 8 0.2016221980930908 1101 9 0.3304560383314268 1101 10-11 0.340301439687245 1101 12-13 0.33391511635063154 1101 14-15 0.3985380644301344 1101 16-17 0.28288013014216773 1101 18-19 0.37579601497542825 1101 20-21 0.3590541718534155 1101 22-23 0.3255387747587193 1101 24-25 0.3530717305691482 1101 26-27 0.3003455360987175 1101 28-29 0.38037578388716753 1101 30-31 0.4420354063382774 1101 32-33 0.5090690040884098 1101 34-35 0.5107162102513101 1101 36-37 0.4767006228069093 1101 38-39 0.565778506843273 1101 40-41 0.6271154819442728 1101 42-43 0.5549980934602416 1101 44-45 0.6185116705821159 1101 46-47 0.6663699934614513 1101 48-49 0.5355147924018766 1101 50-51 0.5945798593118212 1101 52-53 0.5283357413154732 1101 54-55 0.5187177888140013 1101 56-57 0.5364028777028622 1101 58-59 0.5052223512431908 1101 60-61 0.5168005645879106 1101 62-63 0.5369662926401659 1101 64-65 0.4581016181331137 1101 66-67 0.3990731769407745 1101 68-69 0.5647639224121903 1101 70-71 0.5153771275854737 1101 72-73 0.6570003973777503 1101 74-75 0.5938820567603429 1101 76-77 0.6229357490121465 1101 78-79 0.7054920821012836 1101 80-81 0.7052739210232701 1101 82-83 0.7010048551189811 1101 84-85 0.7134251761200261 1101 86-87 0.48143435460899653 1101 88-89 0.5965703509489195 1101 90-91 0.7757930335743133 1101 92-93 0.7638356879631552 1101 94-95 0.7611242440997472 1101 96-97 0.5989520318819856 1101 98-99 0.4551488295014714 1101 100-101 0.3104583431575101 1102 1 0.22565733647030584 1102 2 0.22727760087050086 1102 3 0.2539139457030899 1102 4 0.16352991753577584 1102 5 0.17170210438322187 1102 6 0.1540773176437824 1102 7 0.16511720108744754 1102 8 0.2101687717873162 1102 9 0.24939941106974572 1102 10-11 0.2552114842097595 1102 12-13 0.28898528007590585 1102 14-15 0.2849770842921018 1102 16-17 0.29235901675792064 1102 18-19 0.2728955652277918 1102 20-21 0.3537981406055337 1102 22-23 0.4402558110182113 1102 24-25 0.34391149949478717 1102 26-27 0.33574397156248637 1102 28-29 0.4263664664110465 1102 30-31 0.37632824691291944 1102 32-33 0.43610328202268533 1102 34-35 0.45552751023500093 1102 36-37 0.4725865149650659 1102 38-39 0.4735430504488036 1102 40-41 0.45554833339663503 1102 42-43 0.5949908515593236 1102 44-45 0.5851117498443443 1102 46-47 0.44395794655630283 1102 48-49 0.49019548824322356 1102 50-51 0.40650465779418 1102 52-53 0.3769786733510969 1102 54-55 0.3016368996966037 1102 56-57 0.3466723632958235 1102 58-59 0.3499091643188379 1102 60-61 0.46529098230932675 1102 62-63 0.45277443403634976 1102 64-65 0.39577188016258447 1102 66-67 0.4014848132533473 1102 68-69 0.40848584293812706 1102 70-71 0.3385363934697523 1102 72-73 0.30879096779199955 1102 74-75 0.27652151839611605 1102 76-77 0.23897798768187428 1102 78-79 0.25868509393467676 1102 80-81 0.39089493931665587 1102 82-83 0.36678830145453034 1102 84-85 0.2931271454172233 1102 86-87 0.18564450540953104 1102 88-89 0.2976833543562414 1102 90-91 0.35001656731252595 1102 92-93 0.2991488808381284 1102 94-95 0.36909566090595547 1102 96-97 0.008797952555500643 1102 98-99 -0.14240588344671679 1102 100-101 -0.14870837408940218 1103 1 0.1552398436304685 1103 2 0.17410991525693476 1103 3 0.1828951823925209 1103 4 0.14129323728315057 1103 5 0.14812174527328636 1103 6 0.19538138608763944 1103 7 0.14648011142011796 1103 8 0.16700122386927063 1103 9 0.18838767023109426 1103 10-11 0.12097899908086873 1103 12-13 0.1501600753685537 1103 14-15 0.21539781297683902 1103 16-17 0.11223777584582706 1103 18-19 0.09436011780912423 1103 20-21 0.20180809599217753 1103 22-23 0.20785272572042146 1103 24-25 0.23595476342958221 1103 26-27 0.18648374078478724 1103 28-29 0.23851419535280627 1103 30-31 0.1851580873697003 1103 32-33 0.28260317645612076 1103 34-35 0.3236570771366729 1103 36-37 0.328189231238845 1103 38-39 0.370201983216667 1103 40-41 0.3699504823648425 1103 42-43 0.4724360946378354 1103 44-45 0.4906491754887554 1103 46-47 0.5244111484363074 1103 48-49 0.48117528371946605 1103 50-51 0.47278669495360504 1103 52-53 0.3082881119205325 1103 54-55 0.3820585463515229 1103 56-57 0.4263108666372375 1103 58-59 0.4481836098648486 1103 60-61 0.42122956092429575 1103 62-63 0.36055572305007644 1103 64-65 0.3632877839960287 1103 66-67 0.29347701509662727 1103 68-69 0.25330585193423616 1103 70-71 0.22747060713649603 1103 72-73 0.3603292563283915 1103 74-75 0.15517102361018686 1103 76-77 0.3113496095515984 1103 78-79 0.3626819361172444 1103 80-81 0.3485185669512312 1103 82-83 0.36349737834371254 1103 84-85 0.2978185467905057 1103 86-87 0.19673014284802903 1103 88-89 0.3305466869551985 1103 90-91 0.3594194506568762 1103 92-93 0.10917552492272975 1103 94-95 0.044771631200440254 1103 96-97 -0.11492492142555122 1103 98-99 -0.5128682420793069 1103 100-101 -0.5815072192829085 1104 1 0.05863620217388643 1104 2 0.052217408254129793 1104 3 0.07590988827487166 1104 4 0.017544987983434623 1104 5 0.11571628308841042 1104 6 0.04060197432293222 1104 7 0.08042618985149375 1104 8 0.10649702218859858 1104 9 0.17500356658963767 1104 10-11 0.07097024557947407 1104 12-13 0.12885842971028438 1104 14-15 0.12293020093201079 1104 16-17 0.10626351113994303 1104 18-19 0.06398984469988278 1104 20-21 0.05934923744876386 1104 22-23 0.1765458279613128 1104 24-25 0.12761267659484332 1104 26-27 0.13711836963631896 1104 28-29 0.13830411131919362 1104 30-31 0.08455847252376714 1104 32-33 0.04760230110598229 1104 34-35 0.14695452111426732 1104 36-37 0.19326713740131396 1104 38-39 0.1266401834967752 1104 40-41 0.19277523726680101 1104 42-43 0.19780374169665293 1104 44-45 0.21316318109099797 1104 46-47 0.18698117644751022 1104 48-49 0.25619279072227386 1104 50-51 0.2888843840292381 1104 52-53 0.33053951248076174 1104 54-55 0.2969613824859749 1104 56-57 0.2467152784019433 1104 58-59 0.15749733535503907 1104 60-61 0.20704013515398145 1104 62-63 0.3372670325738838 1104 64-65 0.224172762987628 1104 66-67 0.34535464114704695 1104 68-69 0.30538480851686955 1104 70-71 0.24849214074993142 1104 72-73 0.30696791179057215 1104 74-75 0.4442510306129819 1104 76-77 0.39891088406140085 1104 78-79 0.41879188009483315 1104 80-81 0.5237155207327469 1104 82-83 0.5663335127974953 1104 84-85 0.3798869991714575 1104 86-87 0.49272103920657173 1104 88-89 0.6890200763109426 1104 90-91 0.6381703260070175 1104 92-93 0.6882590333260872 1104 94-95 0.5258684449259299 1104 96-97 0.1465771794147841 1104 98-99 0.17408466408315704 1104 100-101 0.031736828336139666 1105 1 0.07097246905886578 1105 2 0.08103955315793598 1105 3 0.08371194176102392 1105 4 0.1090598604080455 1105 5 0.10640907883135498 1105 6 0.07164901348243546 1105 7 0.10503220704347171 1105 8 0.09762302846503701 1105 9 0.1908191621688431 1105 10-11 0.11843707256814895 1105 12-13 0.16910522327012245 1105 14-15 0.10113024527640135 1105 16-17 0.06912167732905061 1105 18-19 0.12276875382800512 1105 20-21 0.10621784162395898 1105 22-23 0.1235547138939097 1105 24-25 0.12161424570386004 1105 26-27 0.21266841056959862 1105 28-29 0.17828697050871511 1105 30-31 0.12141846365489073 1105 32-33 0.12094109220981153 1105 34-35 0.2898705055596196 1105 36-37 0.15758440442273525 1105 38-39 0.2049647332307032 1105 40-41 0.3256250462448307 1105 42-43 0.3193444532534855 1105 44-45 0.23221207930357224 1105 46-47 0.3728088814550219 1105 48-49 0.2917599724660249 1105 50-51 0.33846524431032066 1105 52-53 0.22242321080249638 1105 54-55 0.2832363688415498 1105 56-57 0.4184291036168375 1105 58-59 0.26963021501120465 1105 60-61 0.26541269621279184 1105 62-63 0.33645860031846553 1105 64-65 0.40148805156724165 1105 66-67 0.32987205484605653 1105 68-69 0.3557822024314632 1105 70-71 0.25403135141988287 1105 72-73 0.2922016578677997 1105 74-75 0.22868401942455563 1105 76-77 0.3788356691616883 1105 78-79 0.4604542828782989 1105 80-81 0.4316565768113385 1105 82-83 0.48296265729197074 1105 84-85 0.5464052827026364 1105 86-87 0.6427385211277077 1105 88-89 0.6375021338904183 1105 90-91 0.6734484164695616 1105 92-93 0.5514178032810619 1105 94-95 0.5187051211436966 1105 96-97 0.46029366529280225 1105 98-99 0.46387351658691145 1105 100-101 0.2642694044038123 1106 1 0.14413882559387758 1106 2 0.09636340555121592 1106 3 0.14992560892039108 1106 4 0.0762251294891172 1106 5 0.06533568844655235 1106 6 0.08752392757835992 1106 7 0.12521611750869965 1106 8 0.04980630750539916 1106 9 0.1560702253065429 1106 10-11 0.1870767027700282 1106 12-13 0.16181067154190742 1106 14-15 0.23741341700642948 1106 16-17 0.17843240373871794 1106 18-19 0.20610144935844232 1106 20-21 0.19600159455187338 1106 22-23 0.21553019675056362 1106 24-25 0.29114018275590325 1106 26-27 0.2764910343950433 1106 28-29 0.2284744845603086 1106 30-31 0.24948737187983738 1106 32-33 0.20901417804373068 1106 34-35 0.3454793442321602 1106 36-37 0.3671238628961859 1106 38-39 0.4137951143338867 1106 40-41 0.40155723504086893 1106 42-43 0.34196588693873053 1106 44-45 0.4650177104653537 1106 46-47 0.31218141096535845 1106 48-49 0.4011936353840113 1106 50-51 0.3620527102077915 1106 52-53 0.4213334448283135 1106 54-55 0.3334887793378698 1106 56-57 0.4595995994926483 1106 58-59 0.5193556408642266 1106 60-61 0.49643813976484097 1106 62-63 0.5718584150367221 1106 64-65 0.5908379390747598 1106 66-67 0.5577192468390777 1106 68-69 0.5534361242017454 1106 70-71 0.5526093698619547 1106 72-73 0.533661955434745 1106 74-75 0.4793395213515197 1106 76-77 0.5007159758811035 1106 78-79 0.6285089680986538 1106 80-81 0.5878740388979402 1106 82-83 0.6772862912377526 1106 84-85 0.6641017618711942 1106 86-87 0.6916108058811901 1106 88-89 0.8304403004941037 1106 90-91 0.9612412676557938 1106 92-93 1.0576422811995307 1106 94-95 1.182852306918143 1106 96-97 0.922500096578311 1106 98-99 0.9758524914144253 1106 100-101 0.9113889270721245 1107 1 -0.008834891694966984 1107 2 0.03836338421640306 1107 3 0.022708344078921527 1107 4 0.0012986012498217292 1107 5 0.006076468696200266 1107 6 -0.02810808957482891 1107 7 -0.06450192516448539 1107 8 0.03011827580186832 1107 9 -0.0047298123849586204 1107 10-11 0.013161311302887668 1107 12-13 0.027788712686096062 1107 14-15 0.04432691856475657 1107 16-17 0.07398182849883739 1107 18-19 0.06727649082797882 1107 20-21 0.07781873387352789 1107 22-23 0.13104767262888828 1107 24-25 0.12279562197746685 1107 26-27 0.1437829444119174 1107 28-29 0.1183584054010467 1107 30-31 0.11165559332096109 1107 32-33 0.06913736880717636 1107 34-35 0.14966732348760559 1107 36-37 0.22198024404972472 1107 38-39 0.16552701218991217 1107 40-41 0.25602736719224595 1107 42-43 0.1545828950512842 1107 44-45 0.1831421670943456 1107 46-47 0.16680488708193053 1107 48-49 0.13655706502623843 1107 50-51 0.190442367446245 1107 52-53 0.1045092561290133 1107 54-55 0.08626563853344749 1107 56-57 0.1086661569961862 1107 58-59 0.11709863993437608 1107 60-61 0.21869577184956057 1107 62-63 0.15532575766136603 1107 64-65 0.020037474687001122 1107 66-67 0.16537071067390485 1107 68-69 0.22987324606441462 1107 70-71 0.13162151470399408 1107 72-73 0.11779867780305153 1107 74-75 0.20205140715338388 1107 76-77 0.0824638659570347 1107 78-79 0.1944413171859658 1107 80-81 0.3204179397195084 1107 82-83 0.3834223611163772 1107 84-85 0.372940019101474 1107 86-87 0.4760567496888477 1107 88-89 0.827195186610652 1107 90-91 0.839808181628495 1107 92-93 0.6813448579115153 1107 94-95 0.9235787750643745 1107 96-97 0.8156537996795024 1107 98-99 0.6667155023609617 1107 100-101 0.36446256239972996 1108 1 -0.07138406584521562 1108 2 -0.03260517716770295 1108 3 -0.016157896989014375 1108 4 0.02549656068299555 1108 5 0.05399923944275997 1108 6 0.01634644612046543 1108 7 0.06770592553431243 1108 8 0.06879465049102151 1108 9 0.05233531426667781 1108 10-11 -0.045312462437657075 1108 12-13 0.04673909519234343 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-0.7039102337944207 2316 82-83 -0.6280829861964676 2316 84-85 -0.5996653147118423 2316 86-87 -0.7091606677015427 2316 88-89 -1.046851821744056 2316 90-91 -1.0465182219473768 2316 92-93 -0.8496739814291097 2316 94-95 -0.4797184095341649 2316 96-97 -0.23663249530017083 2316 98-99 -0.1200417611348179 2316 100-101 0.21879501076403685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 37.0 5 91.0 6 166.0 7 469.0 8 1038.0 9 1882.0 10 3010.0 11 4561.0 12 6255.0 13 8700.0 14 10785.0 15 13917.0 16 17880.0 17 23503.0 18 23580.0 19 18766.0 20 19431.0 21 21686.0 22 22452.0 23 25440.0 24 29891.0 25 35093.0 26 42799.0 27 51580.0 28 63338.0 29 77782.0 30 94489.0 31 117406.0 32 145302.0 33 189150.0 34 262444.0 35 386072.0 36 616432.0 37 926476.0 38 590138.0 39 28556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00541006582897 18.320928320052186 14.3186219412504 24.355039672868443 2 16.172277038919137 19.74132932709033 38.34760514455201 25.73878848943852 3 19.62351279824564 22.703621337882446 29.07396002174916 28.598905842122758 4 14.00944696838899 15.929016314445409 34.52186640760087 35.539670309564734 5 15.335707530517109 36.05528512269978 32.86406003179913 15.744947314983984 6 34.53774020063856 33.91711612349605 16.47313180144359 15.072011874421804 7 30.848114085156713 31.243979683606444 19.30111503088432 18.60679120035252 8 27.382524912320488 34.304374827668845 18.847217318926663 19.465882941084008 9 26.947026369565606 16.65382504297005 19.298718500441943 37.1004300870224 10-11 26.417006604631748 25.34985479087051 26.05555569871747 22.177582905780277 12-13 26.704242374265903 22.388413161848266 26.964291696408555 23.943052767477276 14-15 23.346641563734682 24.107821147278898 24.77818900178222 27.767348287204204 16-17 23.910119493904833 27.445588275653517 25.232926207189166 23.411366023252487 18-19 23.806404513520693 26.029664946746735 26.983446146440492 23.18048439329208 20-21 24.3471418600609 25.33453829868736 26.73206361011073 23.586256231141007 22-23 24.13811230708574 24.980492757581928 26.98080971805979 23.900585217272543 24-25 24.183466001479147 25.30705224178673 26.75860238467664 23.750879372057483 26-27 23.854245557849296 25.618466070660556 26.85050207325706 23.67678629823309 28-29 23.616364437549766 25.811947090792426 27.064288861802655 23.50739960985515 30-31 23.8237287436769 25.314563947420638 26.773857718245324 24.08784959065714 32-33 23.708012400112352 25.426556649598904 26.81761372567715 24.047817224611595 34-35 24.41501624831387 25.522331655266456 26.235335060834704 23.827317035584976 36-37 24.385504561716015 25.177999957735643 26.56860046727888 23.867895013269464 38-39 23.794455830407816 25.407079329026168 26.807436859442923 23.991027981123096 40-41 23.860018175542027 25.311020684375002 26.840505559930005 23.988455580152966 42-43 24.10076072008977 25.29990533679139 26.355824660596898 24.24350928252194 44-45 24.033810029442883 25.362070389501323 26.36872805490026 24.235391526155535 46-47 24.134303354401364 25.775272554291174 26.045707329505234 24.044716761802228 48-49 24.402951920737866 25.5041112283899 26.074926196827704 24.018010654044538 50-51 23.962008525726965 25.694984612589657 26.168943908911167 24.174062952772214 52-53 24.350584844072074 25.702177520166302 25.82968334593016 24.11755428983146 54-55 23.865320142967214 25.358423031430625 26.04060959488328 24.735647230718882 56-57 24.040919661612396 25.870865421002197 26.047063331649305 24.041151585736102 58-59 24.329592825316414 25.29850599150864 26.101127589801763 24.27077359337318 60-61 24.13569488236127 25.401843045197914 26.508182972763024 23.954279099677795 62-63 23.805000660985883 25.676441097396335 26.21797278593566 24.300585455682118 64-65 23.618128911758156 25.51526719524363 26.137669664135103 24.728934228863107 66-67 23.70767751613092 25.7086871561574 26.05070980167711 24.532925526034568 68-69 23.41930773037719 25.506225978787313 26.40437081865491 24.670095472180588 70-71 23.628569171930337 25.65444938481958 26.145003768093645 24.571977675156443 72-73 23.780526772852724 25.448653691043415 26.1206614424021 24.650158093701762 74-75 23.614534828287308 25.90774388562623 25.75691708262361 24.72080420346286 76-77 23.718780684189362 25.632472958513585 25.857206690314282 24.79153966698277 78-79 23.713512123370485 25.68360492335729 26.03616601951655 24.56671693375567 80-81 23.642534650842503 26.069655882310116 25.974593397860207 24.313216068987174 82-83 23.727480988813614 25.75963572737276 25.858859818430606 24.65402346538302 84-85 23.57915379283154 25.55862351537516 25.78077931046923 25.08144338132407 86-87 23.68962414052807 25.382158428793666 26.03294960021907 24.895267830459193 88-89 23.47612624840714 25.475181362810588 25.877772982505647 25.17091940627662 90-91 23.449549453868993 25.644228898262185 25.98571420819208 24.920507439676744 92-93 23.52430416867451 25.19467300244034 26.023782343497544 25.257240485387605 94-95 23.34902244750181 25.157655626306173 26.082329839896307 25.410992086295714 96-97 23.331628274936158 25.122931704036223 26.231442994786903 25.31399702624072 98-99 23.24343846207565 25.202461882896156 25.85491135793962 25.699188297088575 100-101 23.239848110940706 24.505208027994673 26.50537784729174 25.749566013772885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 208.0 1 148.0 2 128.0 3 182.5 4 170.5 5 182.0 6 336.5 7 716.0 8 1201.5 9 1484.5 10 1528.5 11 1640.0 12 1940.0 13 2222.0 14 2494.0 15 2729.5 16 2873.5 17 3028.0 18 3235.0 19 3403.5 20 3631.5 21 4096.5 22 4921.5 23 6311.0 24 7907.5 25 9381.0 26 11544.5 27 14320.5 28 17125.5 29 20609.0 30 24551.5 31 28888.5 32 33635.0 33 37663.5 34 41658.5 35 45043.0 36 50481.5 37 58957.5 38 67081.0 39 71654.5 40 76991.5 41 92247.0 42 108114.0 43 118331.0 44 127420.5 45 138731.5 46 146789.5 47 155032.0 48 175132.5 49 180536.5 50 172446.5 51 169867.0 52 169061.5 53 172823.5 54 173287.0 55 161595.5 56 143547.0 57 129994.0 58 118082.0 59 103159.0 60 88312.0 61 73734.5 62 61480.5 63 50760.5 64 41361.5 65 32911.0 66 25123.0 67 22056.0 68 17345.0 69 11225.5 70 8229.5 71 6150.0 72 4909.5 73 4115.0 74 3312.0 75 2834.5 76 1900.5 77 940.5 78 521.0 79 354.0 80 223.5 81 140.0 82 94.5 83 57.5 84 40.0 85 28.0 86 18.0 87 9.5 88 7.0 89 4.5 90 1.0 91 3.0 92 3.5 93 2.5 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2922478682475178 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013915338052522671 16-17 9.148046312306571E-4 18-19 7.730743362512594E-5 20-21 1.2884572270854325E-5 22-23 0.0 24-25 0.0 26-27 2.576914454170865E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0013786492329814126 36-37 0.007009207315344753 38-39 0.0026026835987125736 40-41 0.0031051819172758922 42-43 2.705760176879408E-4 44-45 0.0011724960766477436 46-47 1.8038401179196054E-4 48-49 4.6384460175075566E-4 50-51 6.442286135427161E-5 52-53 7.730743362512594E-5 54-55 0.0 56-57 7.859589085221139E-4 58-59 0.0019713395574407115 60-61 5.540366076467359E-4 62-63 0.0011080732152934719 64-65 5.15382890834173E-5 66-67 4.896137462924643E-4 68-69 0.00143018752206483 70-71 1.2884572270854325E-5 72-73 0.0 74-75 0.0 76-77 0.2584516351810669 78-79 1.5461486725025189E-4 80-81 1.1596115043768891E-4 82-83 0.0 84-85 0.0 86-87 0.10757329388936276 88-89 0.3745158621969226 90-91 0.27704407296790967 92-93 0.0 94-95 0.0 96-97 0.0 98-99 7.730743362512594E-5 100-101 3.7365259585477544E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3880610.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.81209969054194 #Duplication Level Percentage of deduplicated Percentage of total 1 62.57962072603718 20.533687538590364 2 16.267025239169687 10.675105076323952 3 7.115135407524827 7.003875969102282 4 3.9284480634254133 5.1560251794492435 5 2.3711810132693714 3.8901713895857433 6 1.5668870246404096 3.084771195379066 7 1.0917519918457832 2.5075872635654095 8 0.7693080551276708 2.0194090078068854 9 0.5964543890525422 1.7613828787007975 >10 3.260985055536858 19.36289032906515 >50 0.2540888808369588 5.779801089119096 >100 0.17770886788461154 11.314404017015303 >500 0.01629005195150528 3.6145841538913954 >1k 0.004879145988311015 2.8408087457164095 >5k 2.3608770911182327E-4 0.45549616668882215 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6453 0.16628828972764592 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6152 0.1585317772205916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5035 0.12974764276750306 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 4456 0.11482730807785373 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 4160 0.10719964129350798 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 2.576914454170865E-5 0.0 0.0 0.0 0.0 10-11 2.576914454170865E-5 0.0 0.0 0.0 0.0 12-13 2.576914454170865E-5 0.0 0.0 0.0 0.0 14-15 2.576914454170865E-5 0.0 0.0 5.15382890834173E-5 0.0 16-17 2.576914454170865E-5 0.0 0.0 6.442286135427161E-5 0.0 18-19 2.576914454170865E-5 0.0 0.0 9.019200589598027E-5 0.0 20-21 2.576914454170865E-5 0.0 0.0 1.1596115043768893E-4 0.0 22-23 2.576914454170865E-5 0.0 0.0 1.9326858406281484E-4 0.0 24-25 2.576914454170865E-5 0.0 0.0 3.736525958547754E-4 0.0 26-27 2.576914454170865E-5 0.0 0.0 7.215360471678422E-4 0.0 28-29 2.576914454170865E-5 0.0 0.0 0.0025897990264417193 0.0 30-31 2.576914454170865E-5 0.0 0.0 0.008065742241554808 0.0 32-33 2.576914454170865E-5 0.0 0.0 0.019391281267635757 0.0 34-35 2.576914454170865E-5 0.0 0.0 0.036540646960142864 0.0 36-37 2.576914454170865E-5 0.0 0.0 0.06526035855187715 0.0 38-39 2.576914454170865E-5 0.0 0.0 0.10797271562975924 0.0 40-41 2.576914454170865E-5 0.0 0.0 0.15840293149788306 0.0 42-43 2.576914454170865E-5 0.0 0.0 0.2100700663040089 0.0 44-45 2.576914454170865E-5 0.0 0.0 0.2730240864194031 0.0 46-47 2.576914454170865E-5 0.0 0.0 0.35248324361376177 0.0 48-49 2.576914454170865E-5 0.0 0.0 0.4389516081234651 0.0 50-51 2.576914454170865E-5 0.0 0.0 0.5364620510692906 0.0 52-53 2.576914454170865E-5 0.0 0.0 0.6316403864340916 0.0 54-55 2.576914454170865E-5 0.0 0.0 0.7411979044531658 0.0 56-57 2.576914454170865E-5 0.0 0.0 0.8727880410554011 0.0 58-59 2.576914454170865E-5 0.0 0.0 1.0167473670376566 0.0 60-61 2.576914454170865E-5 0.0 0.0 1.1765933706298752 0.0 62-63 2.576914454170865E-5 0.0 0.0 1.3378695617441587 0.0 64-65 2.576914454170865E-5 0.0 0.0 1.5168620397308672 0.0 66-67 2.576914454170865E-5 0.0 0.0 1.7210309719348247 0.0 68-69 2.576914454170865E-5 0.0 0.0 1.9465238712470463 0.0 70-71 2.576914454170865E-5 0.0 0.0 2.1997443700861465 0.0 72-73 2.576914454170865E-5 0.0 0.0 2.466081363496976 0.0 74-75 2.576914454170865E-5 0.0 0.0 2.7476608059042262 0.0 76-77 2.576914454170865E-5 1.2884572270854325E-5 0.0 3.059647838870693 0.0 78-79 2.576914454170865E-5 2.576914454170865E-5 0.0 3.405108990596839 0.0 80-81 2.576914454170865E-5 2.576914454170865E-5 0.0 3.7797408139441995 0.0 82-83 2.576914454170865E-5 2.576914454170865E-5 0.0 4.17185700186311 0.0 84-85 5.15382890834173E-5 2.576914454170865E-5 0.0 4.5795119839406695 0.0 86-87 5.15382890834173E-5 2.576914454170865E-5 0.0 5.012446496813645 0.0 88-89 5.15382890834173E-5 2.576914454170865E-5 0.0 5.481084159449159 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13965 0.0 25.496326 1 TACATGG 14355 0.0 24.695156 2 ACATGGG 14720 0.0 23.43802 3 GTATCAA 16880 0.0 22.728962 1 GGTATCA 13560 0.0 21.13264 1 CATGGGG 10445 0.0 20.80901 4 GAGTACT 7840 0.0 20.278952 12-13 ATCAACG 19895 0.0 19.201994 3 TCAACGC 19895 0.0 19.17814 4 CAACGCA 20005 0.0 19.048964 5 AGTACTT 8110 0.0 18.813814 12-13 TATCAAC 20360 0.0 18.78675 2 GTACTTT 8390 0.0 18.695034 14-15 AACGCAG 20525 0.0 18.612602 6 AGAGTAC 16240 0.0 17.767597 10-11 ATGGGGA 6260 0.0 17.739275 5 AGTACAT 10945 0.0 17.733805 2 GATGTGC 2800 0.0 17.457159 8 GTATAAC 650 0.0 16.843466 1 GAGTACA 12615 0.0 16.640574 1 >>END_MODULE