FastQCFastQC Report
Fri 27 May 2016
SRR939350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3944809
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66070.167485928976536No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64750.16413975936477532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49200.12472086734744318No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG44470.11273042623863412No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG41830.10603808701511276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG144300.025.7741091
TACATGG147850.024.5049652
GTATCAA170750.024.0070231
ACATGGG150550.023.4355013
CATGGGG105950.021.4393254
TCAACGC201900.020.2463864
ATCAACG202000.020.2131443
CAACGCA202900.020.0998575
TATCAAC206950.019.9817312
AACGCAG208250.019.6390886
GGTATCA135050.019.2389321
GTGTAGG18450.019.0512521
AGTACTT80550.018.85708412-13
AGAGTAC160600.018.69411310-11
GAGTACT77800.018.67080112-13
ACGCAGA229150.017.7653737
AGTACAT112450.017.7543472
GTACTTT82350.017.6970914-15
CGCAGAG232050.017.4820868
GAGTACA125150.017.4208771
Produced by FastQC (version 0.11.5)