FastQCFastQC Report
Fri 27 May 2016
SRR939350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939350_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3944809
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65130.16510305061664582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62560.15858815978162694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51810.13133715726160633No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG45610.1156202999942456No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG40850.10355380957607833No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG40700.10317356302928735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG139450.026.1692621
TACATGG142700.025.0070252
GTATCAA166300.024.8051281
ACATGGG143600.023.9580653
CATGGGG105550.022.254364
GGTATCA128750.021.2118871
ATCAACG195500.020.8018633
TCAACGC195250.020.779894
CAACGCA195700.020.7563535
TATCAAC200800.020.3946022
AACGCAG199900.020.3202536
GAGTACT81150.020.23330112-13
ATGGGGA58950.019.6414785
TAAGGTG14300.019.5787125
AGTACTT86350.018.76754812-13
GTACTTT87400.018.62352214-15
AGAGTAC162800.018.40752210-11
ACGCAGA222250.018.2340987
AGTACAT107400.018.0712072
CGCAGAG225000.017.9057838
Produced by FastQC (version 0.11.5)