##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939350_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3944809 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.297195884515574 33.0 31.0 34.0 31.0 34.0 2 32.50328342893154 34.0 31.0 34.0 31.0 34.0 3 32.59894357369394 34.0 31.0 34.0 31.0 34.0 4 36.128847556370914 37.0 35.0 37.0 35.0 37.0 5 36.05286947986582 37.0 35.0 37.0 35.0 37.0 6 36.08490296995368 37.0 35.0 37.0 35.0 37.0 7 36.02751590761429 37.0 35.0 37.0 35.0 37.0 8 36.00154506846846 37.0 35.0 37.0 35.0 37.0 9 37.71814224719118 39.0 37.0 39.0 35.0 39.0 10-11 37.63331165083024 39.0 37.0 39.0 35.0 39.0 12-13 37.551991617338125 39.0 37.0 39.0 35.0 39.0 14-15 38.880338059459916 40.0 38.0 41.0 35.0 41.0 16-17 38.82085051519604 40.0 38.0 41.0 35.0 41.0 18-19 38.89877190505294 40.0 38.0 41.0 35.0 41.0 20-21 38.889515183117865 40.0 38.0 41.0 35.0 41.0 22-23 38.834543700341385 40.0 38.0 41.0 34.5 41.0 24-25 38.75909987023453 40.0 38.0 41.0 34.0 41.0 26-27 38.59993677767415 40.0 38.0 41.0 34.0 41.0 28-29 38.453168074804125 40.0 38.0 41.0 34.0 41.0 30-31 38.29338606761442 40.0 38.0 41.0 34.0 41.0 32-33 38.12744001040355 40.0 38.0 41.0 33.0 41.0 34-35 38.10995487994476 40.0 38.0 41.0 33.0 41.0 36-37 38.046156987575316 40.0 38.0 41.0 33.0 41.0 38-39 37.956332993561915 40.0 37.5 41.0 33.0 41.0 40-41 37.79694999174865 40.0 37.0 41.0 33.0 41.0 42-43 37.632957514546334 40.0 37.0 41.0 33.0 41.0 44-45 37.44247795013649 40.0 37.0 41.0 32.0 41.0 46-47 37.197194084681925 40.0 36.5 41.0 31.5 41.0 48-49 36.927105849738226 39.5 36.0 41.0 31.0 41.0 50-51 36.68520186908923 39.0 35.5 41.0 31.0 41.0 52-53 36.44390856439438 39.0 35.0 41.0 30.0 41.0 54-55 36.14304368094881 39.0 35.0 40.0 30.0 41.0 56-57 35.87279536220892 38.0 35.0 40.0 29.0 41.0 58-59 35.55590612878849 38.0 34.5 40.0 28.5 41.0 60-61 35.19526230547537 37.5 34.0 40.0 28.0 41.0 62-63 34.797716188540434 37.0 34.0 40.0 28.0 41.0 64-65 34.35854663685872 36.0 34.0 39.0 27.0 41.0 66-67 33.96360711000203 36.0 33.0 39.0 26.0 40.0 68-69 33.565975057347515 35.0 33.0 38.5 26.0 40.0 70-71 33.160199264400376 35.0 33.0 37.5 26.0 40.0 72-73 32.67487906258579 35.0 32.0 37.0 25.0 39.0 74-75 32.18658520602645 35.0 32.0 36.5 24.0 39.0 76-77 30.20703511881057 33.0 29.5 35.0 20.5 36.5 78-79 31.213800338622224 34.0 31.0 35.0 22.0 37.0 80-81 31.283890550847964 34.0 31.0 35.0 23.0 37.0 82-83 31.009053163283696 34.0 31.0 35.0 21.5 36.5 84-85 30.67198449912277 34.0 31.0 35.0 20.0 36.0 86-87 30.163314244111692 34.0 30.5 35.0 18.0 36.0 88-89 29.692597410926613 34.0 30.0 35.0 13.0 35.0 90-91 29.29502062077023 34.0 29.5 35.0 7.0 35.0 92-93 28.966390007729146 34.0 29.0 35.0 3.5 35.0 94-95 28.585699839966907 34.0 29.0 35.0 2.0 35.0 96-97 28.040457725583167 33.0 29.0 35.0 2.0 35.0 98-99 27.291445796235003 33.0 27.0 35.0 2.0 35.0 100-101 23.934772253865773 30.0 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1557832402066026 1101 2 0.1758024521704158 1101 3 0.16309573006490297 1101 4 0.12357846449900478 1101 5 0.17392832717274587 1101 6 0.17248779948808135 1101 7 0.1603579683922831 1101 8 0.16093980169635813 1101 9 0.270101520387243 1101 10-11 0.2126865686476691 1101 12-13 0.2684619558668544 1101 14-15 0.2137256748492149 1101 16-17 0.24327325776587827 1101 18-19 0.23235240561142234 1101 20-21 0.30495310368066697 1101 22-23 0.3228368519072191 1101 24-25 0.3341111279625011 1101 26-27 0.2824455018870182 1101 28-29 0.2808028897051784 1101 30-31 0.2508669233405101 1101 32-33 0.26205281302986094 1101 34-35 0.30621646103889333 1101 36-37 0.3755992295971424 1101 38-39 0.37244174527946683 1101 40-41 0.35287382682821544 1101 42-43 0.3395022434228423 1101 44-45 0.3948414090854797 1101 46-47 0.3948345191287501 1101 48-49 0.40211936694471007 1101 50-51 0.38641178191424785 1101 52-53 0.3285916133575597 1101 54-55 0.3045972575249678 1101 56-57 0.3119947089351669 1101 58-59 0.31491143249051845 1101 60-61 0.3704523950878169 1101 62-63 0.3375467522271478 1101 64-65 0.31048714443242886 1101 66-67 0.2218124399653334 1101 68-69 0.37117625234973417 1101 70-71 0.3410068395561652 1101 72-73 0.22403255476501016 1101 74-75 0.1461519208861759 1101 76-77 0.3413054806714726 1101 78-79 0.35308871619924176 1101 80-81 0.37834573313895703 1101 82-83 0.41398929390099326 1101 84-85 0.28085180896107076 1101 86-87 0.3354513692110501 1101 88-89 0.604455748719591 1101 90-91 0.5635612380187531 1101 92-93 0.4407983333935661 1101 94-95 0.6171226145331978 1101 96-97 0.6489726995065759 1101 98-99 0.5463093156316994 1101 100-101 0.2573741480509497 1102 1 0.14137376022788573 1102 2 0.16200868345273278 1102 3 0.18050774042338702 1102 4 0.13351791626716647 1102 5 0.0779896321093716 1102 6 0.09786667518115166 1102 7 0.15297980043145998 1102 8 0.1571767158573607 1102 9 0.18236404797873007 1102 10-11 0.23006723065378054 1102 12-13 0.1927943396330818 1102 14-15 0.21852162718944612 1102 16-17 0.31891939914409306 1102 18-19 0.2135847147735177 1102 20-21 0.2465181951033273 1102 22-23 0.17619498614806872 1102 24-25 0.23793788798241877 1102 26-27 0.2774945850143524 1102 28-29 0.28551165906858245 1102 30-31 0.21046195791947042 1102 32-33 0.138723754262422 1102 34-35 0.2929202671929261 1102 36-37 0.3579750377922224 1102 38-39 0.28706749433229817 1102 40-41 0.3336551146560538 1102 42-43 0.3307031311686828 1102 44-45 0.30289377890243685 1102 46-47 0.2377471355224685 1102 48-49 0.22051910217702186 1102 50-51 0.22685809753992459 1102 52-53 0.36057137584862176 1102 54-55 0.20889789602426134 1102 56-57 0.28191826042407087 1102 58-59 0.17352132905180895 1102 60-61 0.19224254102205407 1102 62-63 0.19792627562160447 1102 64-65 0.19342780550648087 1102 66-67 0.1886244083022106 1102 68-69 0.3172745129950343 1102 70-71 0.1964326321313834 1102 72-73 0.2341093962412799 1102 74-75 0.22141260094893767 1102 76-77 0.338951095989767 1102 78-79 0.22829260390835415 1102 80-81 0.30554982345222825 1102 82-83 0.2737296071120099 1102 84-85 0.3396931600345745 1102 86-87 0.315437149400946 1102 88-89 0.3361628249757693 1102 90-91 0.49696109568242264 1102 92-93 0.3818974945017821 1102 94-95 0.37452260292196726 1102 96-97 0.27309306967582003 1102 98-99 0.025549054402670635 1102 100-101 -0.016902509699292523 1103 1 0.16433182619157805 1103 2 0.17636787677115962 1103 3 0.21890605980521372 1103 4 0.1049898204439117 1103 5 0.15899775686440876 1103 6 0.08785150871929659 1103 7 0.16486331973224821 1103 8 0.14147829176824445 1103 9 0.1841529709705938 1103 10-11 0.20568941534379803 1103 12-13 0.22382830985682745 1103 14-15 0.3046495215505942 1103 16-17 0.13819732597774248 1103 18-19 0.21780666202603527 1103 20-21 0.2159904363125449 1103 22-23 0.19862250996282427 1103 24-25 0.2568121332095288 1103 26-27 0.2750218931661834 1103 28-29 0.16082412415826752 1103 30-31 0.17348405058825733 1103 32-33 0.25139314622173003 1103 34-35 0.39973054819849807 1103 36-37 0.47375061959927933 1103 38-39 0.4014389950167825 1103 40-41 0.4536859682760337 1103 42-43 0.4523213509933868 1103 44-45 0.48388618809774897 1103 46-47 0.3653620721205968 1103 48-49 0.3460354908550016 1103 50-51 0.36514247911372166 1103 52-53 0.3383377403852279 1103 54-55 0.29226127195370566 1103 56-57 0.2808700515245164 1103 58-59 0.22555123973820912 1103 60-61 0.22993597968118706 1103 62-63 0.20840036482918123 1103 64-65 0.24850984830203515 1103 66-67 0.22178580142031024 1103 68-69 0.07198070776573218 1103 70-71 0.1707031840126021 1103 72-73 0.2618759974579987 1103 74-75 0.2739213628468562 1103 76-77 0.35242950792299155 1103 78-79 0.32816711611063454 1103 80-81 0.36881271447144925 1103 82-83 0.3704669717073159 1103 84-85 0.3500706837546552 1103 86-87 0.23109992946452707 1103 88-89 0.49713654590908263 1103 90-91 0.4789234673307945 1103 92-93 0.44223719673926 1103 94-95 0.36808859596546384 1103 96-97 0.17110150116828748 1103 98-99 0.050180306781303585 1103 100-101 -0.04769667562909774 1104 1 0.09584805685717868 1104 2 0.12151479515421215 1104 3 0.10566305627580874 1104 4 0.04122846448499473 1104 5 0.0956538587377338 1104 6 0.052556263861291086 1104 7 0.05014640888439459 1104 8 0.10793669216690205 1104 9 0.10846174112209184 1104 10-11 0.10514001325677214 1104 12-13 0.1357021287050202 1104 14-15 0.1676880719278273 1104 16-17 0.08175455562297884 1104 18-19 0.0962599458351292 1104 20-21 0.14080007527241634 1104 22-23 0.10851577704494275 1104 24-25 0.07953622913170477 1104 26-27 0.13326858817975307 1104 28-29 0.19158996944545947 1104 30-31 0.15524695477667194 1104 32-33 0.13666472886777825 1104 34-35 0.08908698528275494 1104 36-37 0.08534622277078086 1104 38-39 0.16127054334925361 1104 40-41 0.15246574136056523 1104 42-43 0.25860923123975965 1104 44-45 0.2460951059533798 1104 46-47 0.2282142350079539 1104 48-49 0.16022954964743263 1104 50-51 0.09247701264578012 1104 52-53 0.0949758974817172 1104 54-55 0.13269034374580002 1104 56-57 0.12465450108594922 1104 58-59 0.07267033744903983 1104 60-61 0.05577650308842408 1104 62-63 0.11909313260952814 1104 64-65 -0.01834174328953253 1104 66-67 0.12101580573189352 1104 68-69 0.09618045712985435 1104 70-71 0.08394059099235562 1104 72-73 0.11656785367234335 1104 74-75 0.025876998773831872 1104 76-77 0.15366867356250324 1104 78-79 0.2193086286744439 1104 80-81 0.15805041515812235 1104 82-83 0.23621161054853346 1104 84-85 0.05979650207095588 1104 86-87 0.04042387074284193 1104 88-89 0.3610407705877172 1104 90-91 0.3955863656540224 1104 92-93 0.27035685903002005 1104 94-95 0.2908599267667711 1104 96-97 0.1641714504277303 1104 98-99 0.1517979334950219 1104 100-101 0.061518244861844096 1105 1 0.1155154481291234 1105 2 0.10003400287178721 1105 3 0.04367689971422806 1105 4 0.07063463590772301 1105 5 0.12183409596256922 1105 6 0.05856647078897481 1105 7 0.1461861464935339 1105 8 0.16929357055882832 1105 9 0.10157349143773331 1105 10-11 0.08843258843013757 1105 12-13 0.1016182292072827 1105 14-15 0.16146163313213435 1105 16-17 0.11377803538903208 1105 18-19 0.11442699964399594 1105 20-21 0.18180961153213104 1105 22-23 0.17130767196937313 1105 24-25 0.18153856688860515 1105 26-27 0.22954512480779954 1105 28-29 0.1801001284682826 1105 30-31 0.15685587657678468 1105 32-33 0.180716713086845 1105 34-35 0.15884786553978358 1105 36-37 0.09002463215927747 1105 38-39 0.17924197502309624 1105 40-41 0.22439592376096584 1105 42-43 0.12956011066643214 1105 44-45 0.2676570171228789 1105 46-47 0.22867207767137643 1105 48-49 0.337139878146985 1105 50-51 0.4242782990508047 1105 52-53 0.3489799981372741 1105 54-55 0.2611847473942319 1105 56-57 0.26837579277115964 1105 58-59 0.2071402862014864 1105 60-61 0.160189029888528 1105 62-63 0.15114680307949158 1105 64-65 0.09857961397209891 1105 66-67 0.23204320517960042 1105 68-69 0.29940852043239374 1105 70-71 0.3385117147976615 1105 72-73 0.3216335046141552 1105 74-75 0.3331664569379953 1105 76-77 0.37978635769279734 1105 78-79 0.4758374216211436 1105 80-81 0.5660392841990252 1105 82-83 0.5107107706167504 1105 84-85 0.3737953651290731 1105 86-87 0.3643410400556313 1105 88-89 0.5113831093240115 1105 90-91 0.6818309870034085 1105 92-93 0.6073306348480791 1105 94-95 0.496941543864331 1105 96-97 0.4221540811920157 1105 98-99 0.6753843251097571 1105 100-101 0.6693646768018162 1106 1 0.0676796508329005 1106 2 0.09010871233498818 1106 3 0.09236493829435233 1106 4 0.06363764009778095 1106 5 0.08698232902447245 1106 6 0.04923411249978216 1106 7 0.06795387516773843 1106 8 0.12695899865591542 1106 9 0.04753720398417727 1106 10-11 0.11801714056864654 1106 12-13 0.16136820944286967 1106 14-15 0.13307776879503308 1106 16-17 0.06533112258960472 1106 18-19 0.04494087425858595 1106 20-21 0.13292926525969762 1106 22-23 0.1122207876306831 1106 24-25 0.13595670270962046 1106 26-27 0.21513074422435352 1106 28-29 0.21707360463240377 1106 30-31 0.20547375150761127 1106 32-33 0.11867465442607994 1106 34-35 0.14963350076786242 1106 36-37 0.1573963611497149 1106 38-39 0.21498116389177824 1106 40-41 0.2194668779865907 1106 42-43 0.21371933273933053 1106 44-45 0.26275208392657845 1106 46-47 0.28956740536526837 1106 48-49 0.2073871308942401 1106 50-51 0.2472917783772388 1106 52-53 0.23741485464766754 1106 54-55 0.17644524229501002 1106 56-57 0.21252829494523695 1106 58-59 0.27176140249526526 1106 60-61 0.20428283177010087 1106 62-63 0.3104360749586803 1106 64-65 0.2711221074498482 1106 66-67 0.3009081352135965 1106 68-69 0.3114270594473325 1106 70-71 0.2941115645881567 1106 72-73 0.36387617201869205 1106 74-75 0.36480887926386885 1106 76-77 0.41609594738368116 1106 78-79 0.5650638426820969 1106 80-81 0.5333228639286496 1106 82-83 0.5436631779216761 1106 84-85 0.5490582317588455 1106 86-87 0.5087854625396204 1106 88-89 0.7759338643244043 1106 90-91 0.7158937587640217 1106 92-93 0.6543120167755028 1106 94-95 0.593178993465088 1106 96-97 0.5068062276067486 1106 98-99 0.6389095049658735 1106 100-101 0.42322130578434525 1107 1 0.056264189618509874 1107 2 0.06922296269588912 1107 3 0.07954859913753154 1107 4 0.04601913259991619 1107 5 0.07404888177467228 1107 6 0.11763334061750896 1107 7 0.10380220913939553 1107 8 0.11256280503720006 1107 9 0.0742577354634264 1107 10-11 0.10443775748524331 1107 12-13 0.1997130080758751 1107 14-15 0.2180691514070503 1107 16-17 0.16972746351409285 1107 18-19 0.16342038402495973 1107 20-21 0.16714434642518228 1107 22-23 0.16871697241105466 1107 24-25 0.19349065669129573 1107 26-27 0.19939738241190952 1107 28-29 0.12361508593930637 1107 30-31 0.2097555621902245 1107 32-33 0.23758577012004167 1107 34-35 0.23876049307850167 1107 36-37 0.27371857780705255 1107 38-39 0.26153641828885554 1107 40-41 0.23781270776003538 1107 42-43 0.22330304661258538 1107 44-45 0.24286985489555235 1107 46-47 0.21409013464670323 1107 48-49 0.2808128003989978 1107 50-51 0.1258643117419922 1107 52-53 0.3187545270396157 1107 54-55 0.24762928613321122 1107 56-57 0.3269708898838175 1107 58-59 0.3291182212796997 1107 60-61 0.3225829967525726 1107 62-63 0.3259900919872294 1107 64-65 0.3742420438137515 1107 66-67 0.29889336044413284 1107 68-69 0.3522163974551731 1107 70-71 0.3426432942525963 1107 72-73 0.38463560695372934 1107 74-75 0.4025920746207916 1107 76-77 0.5078350393908693 1107 78-79 0.6038737593070245 1107 80-81 0.6254228550902994 1107 82-83 0.6521339966471906 1107 84-85 0.5691345361515054 1107 86-87 0.5266179316790627 1107 88-89 0.703910545819852 1107 90-91 0.8042610215280668 1107 92-93 0.7642209081522218 1107 94-95 0.8698070905690614 1107 96-97 0.9068305330600097 1107 98-99 1.130598513008767 1107 100-101 1.1283007772512157 1108 1 -0.004310655319983425 1108 2 -0.010497188613378228 1108 3 0.05310606457566536 1108 4 0.05727308839205847 1108 5 0.015326251837251448 1108 6 0.049158206240107916 1108 7 0.04619853048657063 1108 8 0.03343268779953945 1108 9 0.038619816125205375 1108 10-11 0.12274100935945853 1108 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-0.21882205089698203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 17.0 4 46.0 5 102.0 6 177.0 7 460.0 8 1035.0 9 1920.0 10 3068.0 11 4548.0 12 6260.0 13 8671.0 14 10605.0 15 13820.0 16 17835.0 17 23606.0 18 23129.0 19 18422.0 20 18638.0 21 20797.0 22 21453.0 23 23971.0 24 28523.0 25 34070.0 26 40995.0 27 50261.0 28 61611.0 29 75675.0 30 92418.0 31 113817.0 32 142017.0 33 183190.0 34 252421.0 35 365864.0 36 590617.0 37 939869.0 38 706593.0 39 48279.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01325312199649 18.312348863391698 14.3074845301442 24.366913484467613 2 16.209960989239274 19.73563232085508 38.31645587910593 25.73795081079971 3 19.575269677188427 22.673797387909023 29.16371869968863 28.58721423521392 4 14.021388614759294 15.956944937004556 34.52235583522548 35.49931061301067 5 15.315469012568162 36.03662433339612 32.8952048121975 15.752701841838224 6 34.58251083892782 33.87527761166637 16.438362415011728 15.103849134394087 7 30.793176551767143 31.27406168460881 19.324332306076162 18.60842945754788 8 27.40431792768674 34.3216617078292 18.830822987881035 19.443197376603024 9 26.9543088144445 16.63454428338609 19.3031652483048 37.107981653864606 10-11 26.41075905069168 25.3306433847621 26.087663559883378 22.170934004662836 12-13 26.67501772582652 22.36423867416648 26.99571766339004 23.965025936616957 14-15 23.318090141941923 24.10104970907889 24.807222935413282 27.77363721356591 16-17 23.925962561232694 27.444377239030338 25.22899836442415 23.40066183531282 18-19 23.80155794615151 26.028154975310592 27.008747445060077 23.16153963347782 20-21 24.3577192203729 25.337234324906476 26.745464736061997 23.559581718658624 22-23 24.149470861580372 24.982983967031103 26.96624855601374 23.901296615374786 24-25 24.1993230332824 25.3238913735225 26.724303688207456 23.752481904987636 26-27 23.876310392909687 25.602764577782462 26.844626728383957 23.676298300923897 28-29 23.632728479376315 25.786546319479598 27.050485841012833 23.53023936013125 30-31 23.80614625448279 25.320364053113853 26.77915458010768 24.094335112295678 32-33 23.738893315240357 25.42054634330838 26.824999638765778 24.01556070268548 34-35 24.39943046933297 25.50897081782763 26.271078699854726 23.82052001298467 36-37 24.372423102892817 25.166743197600166 26.582104844927873 23.87872885457914 38-39 23.777545697333778 25.38424573778236 26.81674178577409 24.02146677910977 40-41 23.869465287893135 25.355285192890115 26.808347183186974 23.966902336029776 42-43 24.100392273777103 25.304821201518596 26.347445276939812 24.24734124776449 44-45 24.00332236441009 25.354538059273757 26.367091100874802 24.27504847544135 46-47 24.1176844124975 25.810518620124267 26.037640141609046 24.034156825769184 48-49 24.40523736383688 25.5407676265188 26.031146248145347 24.02284876149897 50-51 23.977143126574695 25.713386883877014 26.15977098003984 24.149699009508446 52-53 24.350880955422927 25.697004771841875 25.854540956112437 24.09757331662276 54-55 23.857821760191687 25.379555765564316 26.071515756529656 24.69110671771434 56-57 24.020608351457366 25.8866811912416 26.065232317360902 24.027478139940126 58-59 24.310773765674487 25.31058719028104 26.110022191317857 24.268616852726613 60-61 24.151862370844647 25.36016213078937 26.551667274441648 23.93630822392433 62-63 23.794629967021933 25.673484939323043 26.2378309562879 24.294054137367123 64-65 23.61531917396233 25.54374002486306 26.10211954548865 24.738821255685956 66-67 23.684796906516894 25.737038219087417 26.047433475232896 24.530731399162796 68-69 23.45180465040701 25.47856421925147 26.445712191091324 24.623918939250196 70-71 23.645292287318888 25.670105405623616 26.13362600491253 24.55097630214496 72-73 23.769921433458503 25.464452144577848 26.17069673081764 24.59492969114601 74-75 23.63119481830426 25.94720048549879 25.756937788369477 24.664666907827478 76-77 23.723410161444352 25.634040583221672 25.889408732724284 24.753140522609694 78-79 23.72611703126818 25.653713525800615 26.07866439160933 24.541505051321877 80-81 23.656199644363166 26.05254648926822 26.000984345160138 24.29026952120848 82-83 23.71855012498704 25.76701432185944 25.88631033847266 24.628125214680864 84-85 23.585413134070624 25.561871309865698 25.809170989013662 25.043544567050013 86-87 23.706556652162025 25.380932412260027 26.05497637788282 24.857534557695125 88-89 23.490544221477162 25.484109860381082 25.920843518994115 25.10450239914765 90-91 23.4582371137404 25.65901463843779 26.00791110136442 24.87483714645739 92-93 23.501949017050002 25.221956246804346 26.073632462306794 25.20246227383886 94-95 23.357531378578784 25.152738700403493 26.12539669220994 25.364333228807784 96-97 23.304360236452514 25.18139661514664 26.27616444801257 25.238078700388282 98-99 23.26936487926285 25.23869976974804 25.878806806615984 25.613128544373126 100-101 23.178157664853817 24.706410822556695 26.426600297404327 25.688831215185164 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 221.0 1 163.5 2 134.0 3 193.0 4 196.0 5 187.0 6 357.0 7 781.5 8 1294.5 9 1577.0 10 1585.0 11 1660.5 12 2007.0 13 2349.0 14 2642.0 15 2815.5 16 2945.0 17 3088.0 18 3262.0 19 3464.5 20 3688.0 21 4116.5 22 4907.0 23 6356.0 24 8087.0 25 9623.0 26 11664.0 27 14493.0 28 17344.5 29 20654.5 30 24950.5 31 29745.5 32 34519.0 33 38456.0 34 42439.0 35 46019.0 36 51518.0 37 59948.5 38 68553.5 39 73315.0 40 78531.0 41 94162.5 42 109796.5 43 119832.0 44 129333.0 45 140885.5 46 148826.0 47 157674.0 48 178480.0 49 183349.0 50 174961.5 51 172674.0 52 171813.0 53 175784.0 54 176999.0 55 164607.5 56 145283.0 57 131645.5 58 120088.5 59 104655.0 60 89258.5 61 74997.5 62 62699.0 63 51430.0 64 41753.0 65 33379.0 66 25449.5 67 22345.5 68 17578.0 69 11248.5 70 8173.5 71 6096.0 72 4945.0 73 4221.5 74 3450.5 75 2929.5 76 1902.5 77 907.0 78 518.0 79 351.5 80 219.0 81 124.5 82 79.5 83 61.5 84 40.0 85 19.0 86 10.5 87 9.5 88 6.5 89 4.5 90 4.5 91 3.0 92 1.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27550129803496193 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0024589276692483716 16-17 5.323451655073794E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.6477350360942695E-4 26-27 0.0012041140648381202 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003992588741305345 36-37 0.022054299713877148 38-39 0.007490856971782411 40-41 0.01835323332511156 42-43 0.006147319173120929 44-45 7.097935540098392E-4 46-47 1.1407396403729559E-4 48-49 0.0 50-51 0.0 52-53 2.534976978606569E-5 54-55 0.0 56-57 1.2674884893032844E-5 58-59 5.830447050795108E-4 60-61 0.002344853705211076 62-63 0.0016730848058803356 64-65 2.534976978606569E-5 66-67 0.0 68-69 3.675716618979525E-4 70-71 1.2674884893032844E-5 72-73 0.0 74-75 0.0 76-77 0.23115187579423996 78-79 0.0 80-81 0.00138156245334058 82-83 0.0 84-85 0.0 86-87 0.1043523273243394 88-89 0.3558600682567901 90-91 0.3581922470771082 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.7884746764672254E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3944809.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.75328037906552 #Duplication Level Percentage of deduplicated Percentage of total 1 62.894249561911664 20.599929901322117 2 15.786556615045747 10.341230300651699 3 7.156284534990675 7.031753815407603 4 3.9409692774782714 5.163186868421164 5 2.433426986557253 3.9851358186347117 6 1.5632753233720031 3.0721436985646533 7 1.0948691354554543 2.5102389040370294 8 0.8124949958491292 2.128950112450737 9 0.6120920611081138 1.8043220605746746 >10 3.251028963147815 19.262284934931238 >50 0.2527508927738957 5.693654147010531 >100 0.1806696257555314 11.473382521418909 >500 0.01628991119477159 3.6427102396917244 >1k 0.004809402343230182 2.8350201484809747 >5k 2.3271301660791202E-4 0.4560565284022216 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6513 0.16510305061664582 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6256 0.15858815978162694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5181 0.13133715726160633 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 4561 0.1156202999942456 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 4085 0.10355380957607833 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 4070 0.10317356302928735 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.069953957213138E-5 0.0 12-13 0.0 0.0 0.0 5.069953957213138E-5 0.0 14-15 0.0 0.0 0.0 5.069953957213138E-5 0.0 16-17 0.0 0.0 0.0 5.069953957213138E-5 0.0 18-19 0.0 0.0 0.0 6.337442446516422E-5 0.0 20-21 0.0 0.0 0.0 7.604930935819706E-5 0.0 22-23 0.0 0.0 0.0 1.2674884893032845E-4 0.0 24-25 0.0 0.0 0.0 3.0419723743278825E-4 0.0 26-27 0.0 0.0 0.0 7.478182086889378E-4 0.0 28-29 0.0 0.0 0.0 0.002623701172857799 0.0 30-31 0.0 0.0 0.0 0.008048551907075856 0.0 32-33 0.0 0.0 0.0 0.018441957519362787 0.0 34-35 0.0 0.0 0.0 0.03584457447749688 0.0 36-37 0.0 0.0 0.0 0.06400816870981586 0.0 38-39 0.0 0.0 0.0 0.10715347688569966 0.0 40-41 0.0 0.0 0.0 0.15364495467334413 0.0 42-43 0.0 0.0 0.0 0.20433181936058248 0.0 44-45 0.0 0.0 0.0 0.26501916822842375 0.0 46-47 0.0 0.0 0.0 0.342310616306138 0.0 48-49 0.0 0.0 0.0 0.4247480676504236 0.0 50-51 0.0 0.0 0.0 0.5166916826644838 0.0 52-53 0.0 0.0 0.0 0.6104478062182479 0.0 54-55 0.0 0.0 0.0 0.7160549471469975 0.0 56-57 0.0 0.0 0.0 0.8445402553076715 0.0 58-59 0.0 0.0 0.0 0.9861694191024204 0.0 60-61 0.0 0.0 0.0 1.145442022668271 0.0 62-63 0.0 0.0 0.0 1.3048033504283731 0.0 64-65 0.0 0.0 0.0 1.481934866808507 0.0 66-67 0.0 0.0 0.0 1.685328744687005 0.0 68-69 0.0 0.0 0.0 1.9158468762365937 0.0 70-71 0.0 0.0 0.0 2.167037745046718 0.0 72-73 0.0 0.0 0.0 2.428444571080628 0.0 74-75 0.0 0.0 0.0 2.7085595272166536 0.0 76-77 0.0 0.0 0.0 3.019765975995289 0.0 78-79 0.0 0.0 0.0 3.3627230114309716 0.0 80-81 0.0 0.0 0.0 3.7394205904519078 0.0 82-83 0.0 0.0 0.0 4.131619553697023 0.0 84-85 0.0 0.0 0.0 4.538749531346131 0.0 86-87 0.0 0.0 0.0 4.97325726036419 0.0 88-89 0.0 0.0 0.0 5.446600836694501 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13945 0.0 26.169262 1 TACATGG 14270 0.0 25.007025 2 GTATCAA 16630 0.0 24.805128 1 ACATGGG 14360 0.0 23.958065 3 CATGGGG 10555 0.0 22.25436 4 GGTATCA 12875 0.0 21.211887 1 ATCAACG 19550 0.0 20.801863 3 TCAACGC 19525 0.0 20.77989 4 CAACGCA 19570 0.0 20.756353 5 TATCAAC 20080 0.0 20.394602 2 AACGCAG 19990 0.0 20.320253 6 GAGTACT 8115 0.0 20.233301 12-13 ATGGGGA 5895 0.0 19.641478 5 TAAGGTG 1430 0.0 19.578712 5 AGTACTT 8635 0.0 18.767548 12-13 GTACTTT 8740 0.0 18.623522 14-15 AGAGTAC 16280 0.0 18.407522 10-11 ACGCAGA 22225 0.0 18.234098 7 AGTACAT 10740 0.0 18.071207 2 CGCAGAG 22500 0.0 17.905783 8 >>END_MODULE