FastQCFastQC Report
Fri 27 May 2016
SRR939349_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939349_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926257
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125591.3558871889767095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97271.0501405117586156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67810.7320862352457256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29480.3182701993075356No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21960.2370832285208101No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19870.21451929648035045No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13580.1466115775643261No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12310.1329004801043339No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10040.10839324291206437No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9420.10169963627805242No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9300.10040409951017915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56500.049.0701371
TATAACG502.85678E-547.4146082
GTATCAA95000.037.6791041
TATCAAC121150.029.4311052
ATCAACG121850.029.2620323
TCAACGC121950.029.1991564
CAACGCA123400.028.8176295
AACGCAG126550.028.1524096
TACCTGG2400.027.658522
CATTCCG2750.025.8415459
GTGTAGC3350.025.5082451
GTACATG76900.025.3727951
ACGCAGA142900.024.8650257
TACATGG77000.024.8156972
CGCAGAG143400.024.6792148
ACATACG1351.8189894E-1224.58003416-17
GAGTACT90700.023.8460712-13
ACATGGG75050.023.7546863
GGATGAC4000.023.6880828
GCAGAGT148100.023.3841829
Produced by FastQC (version 0.11.5)