##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939349_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 926257 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04492489665395 33.0 31.0 34.0 30.0 34.0 2 32.084160227668995 33.0 31.0 34.0 30.0 34.0 3 32.080966729536186 34.0 31.0 34.0 30.0 34.0 4 35.620255501442905 37.0 35.0 37.0 35.0 37.0 5 35.67754197809031 37.0 35.0 37.0 35.0 37.0 6 35.802104599479414 37.0 36.0 37.0 35.0 37.0 7 35.7654571031582 37.0 35.0 37.0 35.0 37.0 8 35.76590298372914 37.0 36.0 37.0 35.0 37.0 9 37.393043183479314 39.0 37.0 39.0 35.0 39.0 10-11 37.32215357076924 39.0 37.0 39.0 34.5 39.0 12-13 37.24274364458244 39.0 37.0 39.0 34.0 39.0 14-15 38.41405625004724 40.0 38.0 41.0 34.0 41.0 16-17 38.38039388636199 40.0 38.0 41.0 34.0 41.0 18-19 38.45990097780638 40.0 38.0 41.0 34.0 41.0 20-21 38.48675799481138 40.0 38.0 41.0 34.0 41.0 22-23 38.44955611671491 40.0 38.0 41.0 34.0 41.0 24-25 38.366090620637685 40.0 38.0 41.0 34.0 41.0 26-27 38.2527662409029 40.0 38.0 41.0 34.0 41.0 28-29 38.09339902424489 40.0 38.0 41.0 34.0 41.0 30-31 37.785189207746875 40.0 37.0 41.0 33.5 41.0 32-33 37.406509208567385 40.0 36.0 41.0 33.0 41.0 34-35 37.07183859339254 40.0 35.0 41.0 32.5 41.0 36-37 36.77604757642857 40.0 35.0 41.0 31.5 41.0 38-39 36.18400346772008 38.5 35.0 40.5 30.5 41.0 40-41 36.083904359157344 39.0 35.0 40.5 30.0 41.0 42-43 35.98155155642549 39.0 35.0 41.0 30.0 41.0 44-45 35.58810459732018 40.0 35.0 41.0 27.5 41.0 46-47 34.96574492824345 39.5 35.0 41.0 22.0 41.0 48-49 34.46246344157183 39.0 35.0 41.0 12.0 41.0 50-51 33.26179667198197 38.0 34.0 40.0 6.0 40.5 52-53 33.342071908768304 38.5 33.5 39.5 2.0 40.5 54-55 33.807374735089724 39.0 35.0 40.5 2.0 41.0 56-57 33.735513469803735 39.0 35.0 41.0 2.0 41.0 58-59 33.557494302337254 39.0 34.5 41.0 2.0 41.0 60-61 33.29314920157148 38.5 34.0 40.5 2.0 41.0 62-63 32.92948177449671 38.0 34.0 40.0 2.0 41.0 64-65 32.584728644425894 38.0 33.0 40.0 2.0 41.0 66-67 32.23354479372355 37.0 33.0 40.0 2.0 41.0 68-69 31.820277741490752 37.0 32.5 39.5 2.0 41.0 70-71 31.41624246834302 36.0 32.0 39.0 2.0 41.0 72-73 30.95824377035747 36.0 31.0 39.0 2.0 40.0 74-75 30.465537642360598 35.0 31.0 38.0 2.0 40.0 76-77 29.977282222968356 35.0 30.5 37.0 2.0 39.5 78-79 29.543175922017326 35.0 30.0 37.0 2.0 39.0 80-81 29.090341017665722 35.0 29.5 36.0 2.0 39.0 82-83 28.606234014965608 35.0 29.0 36.0 2.0 37.5 84-85 28.12124118900046 34.0 29.0 35.5 2.0 37.0 86-87 27.660859782975997 34.0 27.5 35.0 2.0 37.0 88-89 27.1844946920779 34.0 26.0 35.0 2.0 36.0 90-91 26.739491307488095 34.0 25.5 35.0 2.0 36.0 92-93 26.26482336975591 34.0 24.5 35.0 2.0 35.5 94-95 25.78102189781022 34.0 21.0 35.0 2.0 35.0 96-97 25.215530354966276 33.0 12.5 35.0 2.0 35.0 98-99 24.515786115516534 33.0 2.0 35.0 2.0 35.0 100-101 22.18953594952589 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30506999083929287 1101 2 0.38167583483591017 1101 3 0.40095168263361103 1101 4 0.29142422856526196 1101 5 0.3406534330343476 1101 6 0.404165211071394 1101 7 0.513333966187659 1101 8 0.21554039188894336 1101 9 0.4319642381175868 1101 10-11 0.44348577433499514 1101 12-13 0.5794025853034626 1101 14-15 0.6753542374783663 1101 16-17 0.6523944087244473 1101 18-19 0.6379405957121875 1101 20-21 0.6519253580336297 1101 22-23 0.6731941185150845 1101 24-25 0.7502358209280757 1101 26-27 0.6671891919758934 1101 28-29 0.6539189193990822 1101 30-31 0.6189796364878362 1101 32-33 0.567521669333992 1101 34-35 0.48036196646824436 1101 36-37 0.6239951191272368 1101 38-39 1.2640457237350518 1101 40-41 0.8813578326705169 1101 42-43 0.870254639107074 1101 44-45 0.6477927183644212 1101 46-47 0.8221764508112344 1101 48-49 0.7487800763283445 1101 50-51 0.6447955954225577 1101 52-53 0.6336107513043174 1101 54-55 0.41008781161591656 1101 56-57 0.4550857775381374 1101 58-59 0.2800382985885861 1101 60-61 0.4769649209382223 1101 62-63 0.4764149435355378 1101 64-65 0.45468400674382536 1101 66-67 0.4617823099748861 1101 68-69 0.6089349891366957 1101 70-71 0.7730056310859901 1101 72-73 0.6174218959107272 1101 74-75 0.5016384009352031 1101 76-77 0.37273060120479684 1101 78-79 0.4156533831627094 1101 80-81 0.4623140468567861 1101 82-83 0.4574376903349595 1101 84-85 0.45219127089124456 1101 86-87 0.46191383666794295 1101 88-89 0.7143667964728095 1101 90-91 0.8735309640357407 1101 92-93 0.7208871923504852 1101 94-95 0.781970217348011 1101 96-97 0.6324098636234865 1101 98-99 0.7589582089892168 1101 100-101 0.6096745109283894 1102 1 0.4402659622019911 1102 2 0.53285894368193 1102 3 0.4727026837257071 1102 4 0.6093771469004139 1102 5 0.4667607015697186 1102 6 0.47904083296193534 1102 7 0.5997130463963671 1102 8 0.5137080899838367 1102 9 0.7485095602872249 1102 10-11 0.6324403914930983 1102 12-13 0.6984415368870103 1102 14-15 0.8015252118746687 1102 16-17 0.6740695695059102 1102 18-19 0.8084963535085734 1102 20-21 0.7837722212783831 1102 22-23 0.7092243332948414 1102 24-25 0.7961920155646496 1102 26-27 0.626954390374145 1102 28-29 0.590422377717239 1102 30-31 0.815051108396581 1102 32-33 0.8587120588498252 1102 34-35 0.7126729724141256 1102 36-37 0.6121731315043704 1102 38-39 1.0605328005461203 1102 40-41 1.0462559032930159 1102 42-43 0.9472810922074189 1102 44-45 1.2409246198326898 1102 46-47 1.252685490976262 1102 48-49 1.0167754652374583 1102 50-51 1.0612954134058157 1102 52-53 0.9484178135540375 1102 54-55 1.1116865253642487 1102 56-57 1.1558714831563819 1102 58-59 1.0824469868545776 1102 60-61 1.0579623727037841 1102 62-63 1.0124876288892537 1102 64-65 0.9908659021448685 1102 66-67 1.0302145390732989 1102 68-69 1.0102515769294378 1102 70-71 1.0029837890765272 1102 72-73 0.9852140327873151 1102 74-75 0.9278397114557677 1102 76-77 0.7698972071992145 1102 78-79 0.8677314076742952 1102 80-81 0.6139491639542278 1102 82-83 0.4007880118978733 1102 84-85 0.46365529224787494 1102 86-87 0.2320691576241387 1102 88-89 0.216663241079047 1102 90-91 0.3578562886322736 1102 92-93 0.3024003582284145 1102 94-95 0.2115904090723184 1102 96-97 0.12082449776982784 1102 98-99 0.0855053513263151 1102 100-101 -0.0014709811235462666 1103 1 0.21825027564091215 1103 2 0.340569168567292 1103 3 0.35375641141529 1103 4 0.29355874807447435 1103 5 0.2917833849703442 1103 6 0.16989348524519698 1103 7 0.39073194212579665 1103 8 0.24118149407719613 1103 9 0.37998346371988134 1103 10-11 0.5228354175866272 1103 12-13 0.4081297847541592 1103 14-15 0.40652195399028557 1103 16-17 0.4628886320877328 1103 18-19 0.48665391332271923 1103 20-21 0.5797875546482487 1103 22-23 0.6676406361136742 1103 24-25 0.6920462815663413 1103 26-27 0.5800910936387353 1103 28-29 0.549064753354358 1103 30-31 0.5184154068553326 1103 32-33 0.4414775459796658 1103 34-35 0.48677522735727763 1103 36-37 0.22474369362644353 1103 38-39 0.45117292915643503 1103 40-41 0.45053119147877396 1103 42-43 0.40110590937007373 1103 44-45 0.05588896578956337 1103 46-47 -0.12267607329540198 1103 48-49 -0.24407450766425143 1103 50-51 -0.13625569275249916 1103 52-53 -0.13430428586318754 1103 54-55 -0.30673011237728076 1103 56-57 -0.06971569722080062 1103 58-59 -0.055150673558173935 1103 60-61 -0.05062337055056787 1103 62-63 -0.18832592878138854 1103 64-65 -0.22059363005946864 1103 66-67 -0.020159356442995602 1103 68-69 -0.2614004140337336 1103 70-71 -0.07921994135333321 1103 72-73 -0.15318591971455575 1103 74-75 -0.12194654697058027 1103 76-77 -0.007091472413733868 1103 78-79 0.08088534423384885 1103 80-81 0.010986316015220154 1103 82-83 -0.3292544014941612 1103 84-85 -0.11228614499674805 1103 86-87 -0.17527904627679902 1103 88-89 -0.1070806465817924 1103 90-91 -0.27941482215406666 1103 92-93 -0.059895415047190426 1103 94-95 -0.25258884764288325 1103 96-97 0.10751360425697598 1103 98-99 0.2154401327435842 1103 100-101 0.307419085069359 1104 1 0.16505886443751194 1104 2 0.19783986142216037 1104 3 0.11374118867993133 1104 4 0.08027147478082242 1104 5 -0.03067558637829393 1104 6 0.03249654360440246 1104 7 0.153652532638624 1104 8 0.11976842456542869 1104 9 0.25432830569583587 1104 10-11 0.1882746655012184 1104 12-13 0.1980854242927137 1104 14-15 0.1626562314466824 1104 16-17 0.1718985518451106 1104 18-19 0.137666827668383 1104 20-21 0.1525958701409138 1104 22-23 0.3130434728909819 1104 24-25 0.14640672831541934 1104 26-27 0.2652559796665841 1104 28-29 0.1375748207840246 1104 30-31 0.2590727469027385 1104 32-33 0.28409415198558463 1104 34-35 0.2702318168474207 1104 36-37 0.16219015323844133 1104 38-39 0.6710172928505003 1104 40-41 0.6507895077795922 1104 42-43 0.46002200946985283 1104 44-45 0.5115821365707305 1104 46-47 0.48775414530366845 1104 48-49 0.47491394450976543 1104 50-51 0.2978751199152754 1104 52-53 0.3347014314788268 1104 54-55 0.23574734372144235 1104 56-57 0.04848972448276356 1104 58-59 0.16540927624794222 1104 60-61 0.2997001125758487 1104 62-63 0.1737790111137869 1104 64-65 -0.11889591302896463 1104 66-67 0.026889328392009304 1104 68-69 -0.07560590038984216 1104 70-71 -0.08974434695400291 1104 72-73 -0.2274658152713087 1104 74-75 -0.17079227865370683 1104 76-77 -0.2780936309270814 1104 78-79 -0.2349783329383648 1104 80-81 -0.35539230397622745 1104 82-83 -0.10520468368782332 1104 84-85 -0.17973564621706473 1104 86-87 -0.14587903657891843 1104 88-89 -0.18595601557884223 1104 90-91 -0.17731449609953032 1104 92-93 -0.010128384612006158 1104 94-95 -0.05614429675030763 1104 96-97 -0.20915749502728787 1104 98-99 -0.27897476908005814 1104 100-101 -0.14192417732740026 1105 1 0.18169132387289721 1105 2 0.26341125126841547 1105 3 0.2626297763730676 1105 4 0.2062831697814005 1105 5 0.22985765917799483 1105 6 0.08895783779950506 1105 7 0.23528518569985124 1105 8 0.16118499119207996 1105 9 0.20557331983052052 1105 10-11 0.41899437273894335 1105 12-13 0.31370909311508655 1105 14-15 0.43707729019276087 1105 16-17 0.3801818651704423 1105 18-19 0.2625022457065711 1105 20-21 0.2440743970355399 1105 22-23 0.31097651707639784 1105 24-25 0.2469566256937199 1105 26-27 0.2836020277632372 1105 28-29 0.2865844246255591 1105 30-31 0.27056444035431326 1105 32-33 0.3430386975457509 1105 34-35 0.23458530017624213 1105 36-37 0.33829769302855084 1105 38-39 0.7788765655918581 1105 40-41 0.7123092769775923 1105 42-43 0.41197375791115576 1105 44-45 0.45696996912310794 1105 46-47 0.5383195327489716 1105 48-49 0.6601799864103981 1105 50-51 0.672909004436832 1105 52-53 0.6482252280942475 1105 54-55 0.8429724918452024 1105 56-57 0.7249070656127614 1105 58-59 0.755948717831032 1105 60-61 0.7766586379214715 1105 62-63 0.7396553616540018 1105 64-65 0.6991877720579751 1105 66-67 0.7998485120654806 1105 68-69 0.7057372820444208 1105 70-71 0.7422368326145978 1105 72-73 0.7324972021937448 1105 74-75 0.7107425933596154 1105 76-77 0.4269174057456553 1105 78-79 0.5217453242664511 1105 80-81 0.31283950487634726 1105 82-83 0.33317389354952454 1105 84-85 0.35927511937949674 1105 86-87 0.3022195576975051 1105 88-89 0.42515133058542887 1105 90-91 0.7154748777145095 1105 92-93 0.8722584410860144 1105 94-95 0.9689012053214867 1105 96-97 0.9785433917790449 1105 98-99 0.8302350438773836 1105 100-101 0.8966364081971356 1106 1 0.17776707272082604 1106 2 0.21441211532371796 1106 3 0.3021603063269893 1106 4 0.14353115365236846 1106 5 0.28501318913190943 1106 6 0.22090628249996058 1106 7 0.16497268569985124 1106 8 0.03283677690636466 1106 9 0.10591894195034257 1106 10-11 0.22641804785415331 1106 12-13 0.2695702682302894 1106 14-15 0.28057527406372884 1106 16-17 0.2574140080275882 1106 18-19 0.23046019501532555 1106 20-21 0.17952226569914131 1106 22-23 0.18801064726072525 1106 24-25 0.16749954044026794 1106 26-27 0.05394332960656101 1106 28-29 0.11526746033984381 1106 30-31 0.08167835164463355 1106 32-33 0.1416778104489751 1106 34-35 -0.019716975169380646 1106 36-37 -0.15695000282398297 1106 38-39 0.2617754711218083 1106 40-41 0.02101103389003356 1106 42-43 0.034831620814387065 1106 44-45 0.15374056244107237 1106 46-47 -0.08378228176716362 1106 48-49 -0.1900864306402994 1106 50-51 -0.5510613296645488 1106 52-53 -0.4253994838873254 1106 54-55 -0.23977880423774423 1106 56-57 -0.16620396549323146 1106 58-59 -0.2820697153486975 1106 60-61 -0.020054351709859475 1106 62-63 -0.180545531203137 1106 64-65 -0.09671516573004624 1106 66-67 -0.28218273793452653 1106 68-69 -0.3261967617343835 1106 70-71 -0.27335216508125626 1106 72-73 -0.2943785178523406 1106 74-75 -0.09075870272333475 1106 76-77 -0.13344045715757247 1106 78-79 0.0402505085982483 1106 80-81 -0.10098898014669544 1106 82-83 -0.002277575344486138 1106 84-85 0.04632509057765688 1106 86-87 0.037278601476305795 1106 88-89 -0.04556079499060672 1106 90-91 0.06799143877441693 1106 92-93 -0.014100285872508778 1106 94-95 0.06967165463024116 1106 96-97 0.12528878348411254 1106 98-99 0.24728573512162555 1106 100-101 0.24440066510957692 1107 1 0.18643062384361286 1107 2 0.07917834999484086 1107 3 0.14342931811230386 1107 4 0.16051360507776025 1107 5 0.2226177041820776 1107 6 0.2052800382731732 1107 7 0.24754828491947478 1107 8 -0.04163392901814689 1107 9 0.1387864367743532 1107 10-11 0.2046129733996267 1107 12-13 0.29292938194266327 1107 14-15 0.32714543850074307 1107 16-17 0.17306289718191437 1107 18-19 0.26588333166128564 1107 20-21 0.07894768176857525 1107 22-23 -0.003928131804414647 1107 24-25 0.10164634924740312 1107 26-27 0.1120997338906804 1107 28-29 0.03584784236723948 1107 30-31 0.051679421679672544 1107 32-33 0.18339300196036135 1107 34-35 0.1819777861381482 1107 36-37 0.16338862593855907 1107 38-39 0.5154446959187524 1107 40-41 0.42218769916316745 1107 42-43 -0.09177529808193441 1107 44-45 0.22251333179129063 1107 46-47 0.038351641827361505 1107 48-49 -0.14921731334455046 1107 50-51 -0.17788909881569026 1107 52-53 -0.062499589397752686 1107 54-55 -0.11841505702481214 1107 56-57 -0.24549542810670033 1107 58-59 -0.140844998037025 1107 60-61 -0.20770552526456498 1107 62-63 -0.11957779649219447 1107 64-65 -0.22274414128347786 1107 66-67 -0.3184227094666099 1107 68-69 -0.04921072693584705 1107 70-71 -0.12307072098187533 1107 72-73 0.0359157915520214 1107 74-75 -0.15755688416146185 1107 76-77 -0.2921795328463759 1107 78-79 -0.3651260441967814 1107 80-81 -0.3819534300989176 1107 82-83 -0.5341963472428795 1107 84-85 -0.3730745202282044 1107 86-87 -0.09339382844883914 1107 88-89 -0.0870314959382128 1107 90-91 0.011831396371167813 1107 92-93 -0.061813272614028136 1107 94-95 -0.12305534210490521 1107 96-97 -0.02675075306629182 1107 98-99 -0.0016591605624007855 1107 100-101 0.334715977062114 1108 1 0.19653999743124473 1108 2 0.23945434505344565 1108 3 0.166950364242048 1108 4 0.19192521735893564 1108 5 0.17031724318596275 1108 6 0.15011115701733502 1108 7 0.21366356407823162 1108 8 0.3363973464044321 1108 9 0.31562936666076524 1108 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Count 2 4322.0 3 640.0 4 614.0 5 607.0 6 714.0 7 888.0 8 1214.0 9 1491.0 10 1958.0 11 2952.0 12 4628.0 13 7289.0 14 7916.0 15 10383.0 16 14505.0 17 20268.0 18 16422.0 19 8282.0 20 6820.0 21 6473.0 22 6616.0 23 7190.0 24 7795.0 25 8793.0 26 9889.0 27 11138.0 28 12884.0 29 15072.0 30 17858.0 31 21548.0 32 26290.0 33 33763.0 34 45611.0 35 66732.0 36 96017.0 37 170057.0 38 210498.0 39 40105.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46989060777549 22.259184420433453 16.03134278901443 19.239582182776626 2 14.427779977112499 23.345901990433976 42.197589673366345 20.028728359087182 3 17.1713719250692 30.564512029316422 32.66340952675567 19.60070651885871 4 10.739180059177679 19.752146137493444 41.31110474016455 28.19756906316433 5 10.164703183698908 40.95095476342988 35.46149784464103 13.422844208230181 6 24.580253825651045 41.443382093856265 19.550973733303646 14.425390347189046 7 23.330281249358144 37.66851773577889 23.47935411128684 15.521846903576128 8 24.34268069726282 35.598547351099704 21.78670625160147 18.272065700036002 9 26.88889465524064 17.075140608883498 23.536770223136187 32.49919451273967 10-11 22.102093401749464 29.68842480558644 28.897419584649285 19.312062208014808 12-13 24.009860321125213 28.858626213623715 29.52070105348267 17.610812411768396 14-15 21.714824412609556 28.962973293505488 29.880451598465267 19.441750695419692 16-17 17.31031128404669 32.32495676610462 30.735192390834413 19.629539559014265 18-19 16.95992008125937 29.47900916762664 36.178906836609315 17.382163914504677 20-21 18.665304069946632 28.151843020206446 36.46925235615683 16.713600553690096 22-23 18.414300538195207 28.535809474679734 35.94741660353566 17.102473383589402 24-25 17.590074071269594 29.172722085353687 36.4264060425281 16.810797800848615 26-27 17.268709936634043 29.078852166652787 36.66859897320979 16.983838923503374 28-29 16.87412500060834 28.994093399239816 36.90692683180058 17.22485476835126 30-31 17.398464978088494 29.065821025853143 36.489238896233275 17.046475099825088 32-33 17.05371989087851 29.494272203491562 36.27337689901396 17.178631006615962 34-35 17.490575247150904 29.377492297949498 36.25145386868566 16.88047858621394 36-37 17.76982791302853 29.10612292050503 35.644928860702784 17.479120305763658 38-39 17.120148517723504 30.012486723029518 35.592528352668864 17.27483640657811 40-41 17.269274869684722 29.664067137780453 36.0483365996861 17.01832139284872 42-43 17.250532224672018 29.705023354983823 35.27638267252159 17.768061747822568 44-45 17.44879787252503 30.096894684783177 34.72634355423673 17.727963888455065 46-47 17.901642295786775 30.30177808724927 33.82047978203952 17.976099834924437 48-49 17.761164726287802 30.576776848656955 33.399645731256896 18.262412693798343 50-51 17.737820013265022 30.748877272502952 32.879741120541965 18.633561593690057 52-53 17.81804040931064 31.309378598240684 32.63496843175146 18.23761256069722 54-55 17.97423792341655 31.08004637477455 32.35578648949681 18.58992921231209 56-57 17.78849880608099 31.44102365341927 32.25538212184208 18.515095418657662 58-59 17.71867511706197 30.901432711658718 32.58303825063159 18.79685392064773 60-61 18.03912921460931 31.289339694503465 32.226720165219945 18.44481092566728 62-63 17.692258464101666 30.875951679261714 32.89027391658522 18.541515940051394 64-65 17.704830725042626 30.807640910609457 32.55598026752515 18.93154809682276 66-67 17.540537784643153 31.107352992224378 32.10235655026381 19.24975267286865 68-69 17.616917865250517 30.81510934393638 32.28888605610539 19.279086734707715 70-71 17.81041479763469 30.917811770806324 32.31162598459791 18.960147446961077 72-73 17.980357551571192 30.385049697303668 32.2785695767675 19.356023174357645 74-75 17.655255306073855 31.103076495005393 31.704642160364354 19.537026038556395 76-77 17.71219072831594 30.822484953617717 32.00569807676353 19.459626241302814 78-79 17.670519855125896 30.5501095230866 31.942137109891778 19.837233511895725 80-81 17.808679660204596 30.608424184723088 31.61812704808708 19.964769106985237 82-83 18.123906013105067 30.442062887843434 31.68663226640465 19.747398832646844 84-85 17.87380174888539 30.206881667720452 31.531288737442697 20.38802784595146 86-87 17.80124926184665 30.240346526734303 31.747290997538673 20.211113213880374 88-89 18.394404341429315 29.783497948211522 31.599440564294706 20.222657146064453 90-91 18.3202831764711 29.546347040879645 31.820632482415373 20.31273730023388 92-93 18.66725754055794 29.6025649857317 31.645961671666857 20.084215802043506 94-95 18.693900399002818 28.86521775920538 31.73526087060289 20.705620971188914 96-97 19.016283895855096 28.61744723273572 31.386718389813318 20.97955048159587 98-99 18.98125397977326 28.822345091224268 31.068591493834674 21.127809435167798 100-101 19.46518687711695 28.116272587560182 31.638690141135307 20.779850394187562 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3007.0 1 1874.0 2 932.0 3 1131.5 4 958.0 5 975.5 6 1629.0 7 3100.0 8 4692.0 9 5091.5 10 4344.5 11 3683.5 12 3707.5 13 3814.0 14 3874.5 15 3981.5 16 4109.0 17 4334.0 18 4645.0 19 4880.0 20 5283.5 21 5840.5 22 6492.0 23 7381.0 24 8297.5 25 9706.0 26 12312.0 27 15345.0 28 16465.0 29 17601.5 30 20802.5 31 23929.5 32 27635.5 33 32785.0 34 37860.5 35 40530.5 36 42995.5 37 45769.5 38 44056.0 39 40640.0 40 37661.5 41 34556.5 42 31614.0 43 30130.5 44 29450.0 45 28238.0 46 26276.5 47 23624.5 48 23401.5 49 21781.5 50 18054.5 51 15052.5 52 12247.0 53 10487.5 54 9475.0 55 8612.0 56 7575.5 57 6353.0 58 5092.5 59 4129.0 60 3506.0 61 2933.5 62 2416.5 63 1825.0 64 1360.0 65 1102.5 66 882.0 67 719.0 68 561.0 69 404.5 70 319.0 71 266.0 72 230.0 73 199.0 74 173.0 75 145.5 76 100.5 77 67.5 78 59.0 79 47.0 80 36.0 81 29.0 82 20.0 83 17.5 84 12.5 85 6.5 86 6.5 87 3.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 1.0 96 2.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3013202599278602 2 0.18731302435501163 3 0.18612544898446112 4 0.2075018056543702 5 0.22121290311436242 6 0.11389927417552581 7 0.13020144517126456 8 0.14315681284999734 9 0.1458558477830667 10-11 0.13295446080299528 12-13 0.12253618596134766 14-15 0.13775874298385868 16-17 0.11411519697017135 18-19 0.14223914097275378 20-21 0.16755608864494412 22-23 0.17192852523651642 24-25 0.1953561484555582 26-27 0.1757611548414749 28-29 0.17419571458029467 30-31 0.16350753624534012 32-33 0.17268425501777585 34-35 0.3550310550959399 36-37 0.471629364204535 38-39 0.44021259758360803 40-41 0.4290925736593624 42-43 0.4517104863984833 44-45 0.45613690368871707 46-47 0.4593757456084003 48-49 0.4704957695326459 50-51 0.46245264543209924 52-53 0.4713054800125667 54-55 0.45386971434493883 56-57 0.418296433927085 58-59 0.4076082555921305 60-61 0.3633980633884548 62-63 0.4679046959968993 64-65 0.4669330434209944 66-67 0.4751381096175252 68-69 0.48685192122704607 70-71 0.4787008357291767 72-73 0.47243907468445584 74-75 0.476379665686737 76-77 0.4525201968784041 78-79 0.4401586168849466 80-81 0.43778346614384556 82-83 0.4503069882332873 84-85 0.4517104863984833 86-87 0.45262815827572694 88-89 0.45953768770438436 90-91 0.4781610287425628 92-93 0.48134588996358463 94-95 0.481399870662246 96-97 0.47783714455059445 98-99 0.47589383939878455 100-101 0.45710855626462205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 926257.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.81300468745915 #Duplication Level Percentage of deduplicated Percentage of total 1 73.4955484314393 28.525830657768346 2 12.771946999601377 9.914352775270164 3 4.9288941237771615 5.739155721904585 4 2.504494675943749 3.888278543884849 5 1.4785519818622814 2.8693522501336375 6 0.9278014666504915 2.1606457604482205 7 0.6180968661538083 1.6793137594332104 8 0.45693976198828623 1.4188164099150258 9 0.33059510324881775 1.1548250362842667 >10 2.014979743005979 15.443081097852554 >50 0.27310170660022387 7.447585388352013 >100 0.18930547554620153 13.689607727380437 >500 0.007238150535410545 1.7696704317923286 >1k 0.0016703424312485876 1.1587462272891031 >5k 5.567808104161958E-4 1.7837171503252998 >10k+ 2.783904052080979E-4 1.3570210619660432 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12559 1.3558871889767095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9727 1.0501405117586156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6781 0.7320862352457256 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2948 0.3182701993075356 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2196 0.2370832285208101 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1987 0.21451929648035045 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1358 0.1466115775643261 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1231 0.1329004801043339 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1004 0.10839324291206437 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 942 0.10169963627805242 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 930 0.10040409951017915 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.619420959841599E-4 0.0 20-21 0.0 0.0 0.0 3.7786489062970643E-4 0.0 22-23 0.0 0.0 0.0 0.0016734016585029858 0.0 24-25 0.0 0.0 0.0 0.004912243578186184 0.0 26-27 0.0 0.0 0.0 0.008528950388499087 0.0 28-29 0.0 0.0 0.0 0.01765168846227343 0.0 30-31 0.0 0.0 0.0 0.04329252032643208 0.0 32-33 0.0 0.0 0.0 0.0876646546260919 0.0 34-35 0.0 0.0 0.0 0.15940500314707473 0.0 36-37 0.0 0.0 0.0 0.27038931959488566 0.0 38-39 0.0 0.0 0.0 0.43519239260809905 0.0 40-41 0.0 0.0 0.0 0.6140844279719344 0.0 42-43 0.0 0.0 0.0 0.7934083089250608 0.0 44-45 0.0 0.0 0.0 1.0066860493361993 0.0 46-47 0.0 0.0 0.0 1.2567786262344036 0.0 48-49 0.0 0.0 0.0 1.530082903557004 0.0 50-51 0.0 0.0 0.0 1.8073817525805473 0.0 52-53 0.0 0.0 0.0 2.079174570340629 0.0 54-55 0.0 0.0 0.0 2.3847593054627385 0.0 56-57 0.0 0.0 0.0 2.744594642739542 0.0 58-59 0.0 0.0 0.0 3.1458331758896287 0.0 60-61 0.0 0.0 0.0 3.579784012428516 0.0 62-63 0.0 0.0 0.0 4.0023449215498506 0.0 64-65 0.0 0.0 0.0 4.4448247084772365 0.0 66-67 0.0 0.0 0.0 4.948626569083958 0.0 68-69 0.0 0.0 0.0 5.5059772827627755 0.0 70-71 0.0 0.0 0.0 6.125999587587462 0.0 72-73 0.0 0.0 0.0 6.744564413548291 0.0 74-75 0.0 5.398069866138663E-5 0.0 7.381212773560685 0.0 76-77 0.0 1.0796139732277327E-4 0.0 8.059210348747701 0.0 78-79 0.0 1.0796139732277327E-4 0.0 8.822605389217031 0.0 80-81 0.0 1.0796139732277327E-4 0.0 9.647322503365697 0.0 82-83 0.0 1.0796139732277327E-4 0.0 10.478301378559083 0.0 84-85 0.0 1.0796139732277327E-4 0.0 11.29724255795098 0.0 86-87 0.0 1.0796139732277327E-4 0.0 12.158450624394742 0.0 88-89 0.0 1.0796139732277327E-4 0.0 13.071318219457451 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5650 0.0 49.070137 1 TATAACG 50 2.85678E-5 47.414608 2 GTATCAA 9500 0.0 37.679104 1 TATCAAC 12115 0.0 29.431105 2 ATCAACG 12185 0.0 29.262032 3 TCAACGC 12195 0.0 29.199156 4 CAACGCA 12340 0.0 28.817629 5 AACGCAG 12655 0.0 28.152409 6 TACCTGG 240 0.0 27.65852 2 CATTCCG 275 0.0 25.841545 9 GTGTAGC 335 0.0 25.508245 1 GTACATG 7690 0.0 25.372795 1 ACGCAGA 14290 0.0 24.865025 7 TACATGG 7700 0.0 24.815697 2 CGCAGAG 14340 0.0 24.679214 8 ACATACG 135 1.8189894E-12 24.580034 16-17 GAGTACT 9070 0.0 23.84607 12-13 ACATGGG 7505 0.0 23.754686 3 GGATGAC 400 0.0 23.688082 8 GCAGAGT 14810 0.0 23.384182 9 >>END_MODULE