FastQCFastQC Report
Fri 27 May 2016
SRR939349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939349_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926257
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119901.2944571539000516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102151.1028256736521291No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71430.7711682610765696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29630.3198896202673772No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22640.24442460353875867No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.22186067149829905No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13180.14229312167141517No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11190.12080880360418329No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.11389927417552581No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA9750.10526236238970395No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT9400.10148371348340689No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9370.10115982929143855No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.10018817671553361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59250.047.4016761
GTATCAA98900.037.7354741
TATCAAC126550.029.3623812
ATCAACG128700.028.8349933
TCAACGC129250.028.749014
ATGGGCG1004.9834227E-528.4736375
CAACGCA130650.028.4409455
AACGCAG132750.027.9195356
CGCCGAA700.00842870627.117757
GTACATG77350.026.6475721
ACATGGG75850.025.6519243
TACATGG79150.025.6016882
CATGGGT7600.024.9768734
ACGCAGA148650.024.7735637
CGCAGAG148550.024.630518
GAGTACT94100.024.2071312-13
CATGGGG23750.024.1776144
GCAGAGT152950.023.7357889
CATGGGA43050.023.3697684
CTCCGAC1850.023.08673394-95
Produced by FastQC (version 0.11.5)