##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939349_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 926257 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20569129302127 33.0 31.0 34.0 30.0 34.0 2 32.39778376843576 34.0 31.0 34.0 31.0 34.0 3 32.445272748276125 34.0 31.0 34.0 30.0 34.0 4 36.03589716460982 37.0 35.0 37.0 35.0 37.0 5 35.9968788360034 37.0 35.0 37.0 35.0 37.0 6 36.05378097007634 37.0 35.0 37.0 35.0 37.0 7 35.9986893486365 37.0 35.0 37.0 35.0 37.0 8 35.98763518116462 37.0 35.0 37.0 35.0 37.0 9 37.64560483753429 39.0 37.0 39.0 35.0 39.0 10-11 37.58212947378535 39.0 37.0 39.0 35.0 39.0 12-13 37.512086818237265 39.0 37.0 39.0 35.0 39.0 14-15 38.687252026165524 40.0 38.0 41.0 34.0 41.0 16-17 38.69749108508762 40.0 38.0 41.0 34.5 41.0 18-19 38.916646243969005 40.0 38.0 41.0 35.0 41.0 20-21 38.94962629162316 40.0 38.0 41.0 35.0 41.0 22-23 38.84367729474649 40.0 38.0 41.0 35.0 41.0 24-25 38.76774264593952 40.0 38.0 41.0 34.5 41.0 26-27 38.57735758002369 40.0 38.0 41.0 34.0 41.0 28-29 38.37690241477257 40.0 38.0 41.0 34.0 41.0 30-31 38.03990847032735 40.0 37.0 41.0 34.0 41.0 32-33 37.73315829192114 40.0 36.0 41.0 33.0 41.0 34-35 37.525210605695825 40.0 36.0 41.0 33.0 41.0 36-37 37.278424886397616 40.0 35.0 41.0 33.0 41.0 38-39 37.06125621722697 40.0 35.0 41.0 32.5 41.0 40-41 36.77891665056242 40.0 35.0 41.0 31.0 41.0 42-43 36.428143053169904 40.0 35.0 41.0 30.5 41.0 44-45 35.909602842407665 40.0 35.0 41.0 29.5 41.0 46-47 35.228549959676414 39.0 35.0 41.0 25.0 41.0 48-49 34.60551877070834 39.0 35.0 41.0 20.5 41.0 50-51 34.2522258941093 39.0 35.0 41.0 13.5 41.0 52-53 33.98531671015712 39.0 34.5 40.0 8.5 41.0 54-55 33.73461900962692 38.5 34.0 40.0 6.0 41.0 56-57 33.42899055013889 38.0 34.0 40.0 2.0 41.0 58-59 33.13496524182813 38.0 33.0 40.0 2.0 41.0 60-61 32.82966498498797 38.0 33.0 40.0 2.0 41.0 62-63 32.499211341992556 37.5 33.0 40.0 2.0 41.0 64-65 32.228442538086085 37.0 32.5 40.0 2.0 41.0 66-67 31.827345974173475 36.5 32.0 39.5 2.0 41.0 68-69 31.36098998442117 36.0 31.0 39.0 2.0 40.0 70-71 30.907492736896995 35.5 31.0 39.0 2.0 40.0 72-73 30.419895342221434 35.0 30.5 38.0 2.0 40.0 74-75 29.88392854251034 35.0 29.5 37.0 2.0 39.5 76-77 27.960832144858287 33.0 27.0 35.5 2.0 38.0 78-79 28.836409873285707 34.0 29.0 36.0 2.0 39.0 80-81 28.78255548945919 34.0 29.0 36.0 2.0 38.0 82-83 28.394766247380588 34.0 29.0 35.5 2.0 37.0 84-85 27.918495082898158 34.0 27.5 35.0 2.0 37.0 86-87 27.32805743978183 34.0 26.0 35.0 2.0 36.0 88-89 26.731776925842397 33.5 25.5 35.0 2.0 36.0 90-91 26.25227069808919 33.0 24.5 35.0 2.0 35.5 92-93 25.8831479816077 33.0 23.5 35.0 2.0 35.0 94-95 25.38686293328957 32.5 20.0 35.0 2.0 35.0 96-97 24.726297884928265 32.0 10.0 34.0 2.0 35.0 98-99 23.857603775194143 32.0 2.0 34.0 2.0 35.0 100-101 19.96739619781551 27.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3261585513067189 1101 2 0.2681267007075121 1101 3 0.2704163704491691 1101 4 0.240915927428496 1101 5 0.25269651770788926 1101 6 0.2458764428057023 1101 7 0.25080827056825683 1101 8 0.24936885872803316 1101 9 0.3450457215499654 1101 10-11 0.3787284095695611 1101 12-13 0.4408860989767689 1101 14-15 0.48446941856692405 1101 16-17 0.33506739032571176 1101 18-19 0.485653101467868 1101 20-21 0.36103092467925535 1101 22-23 0.44492795743285285 1101 24-25 0.5210372848638585 1101 26-27 0.5301015175671893 1101 28-29 0.3276093578653203 1101 30-31 0.5035255272419619 1101 32-33 0.7933677577486762 1101 34-35 0.8178668367850292 1101 36-37 0.9488250459528516 1101 38-39 1.0626120514692161 1101 40-41 1.2471496773040158 1101 42-43 1.404864890449808 1101 44-45 1.3728309367625613 1101 46-47 1.2705326425224257 1101 48-49 1.0732703564730741 1101 50-51 0.8376659509616431 1101 52-53 0.7788896767268341 1101 54-55 0.8413027383056786 1101 56-57 0.7758080907061782 1101 58-59 0.6230841817861617 1101 60-61 0.7312057542507802 1101 62-63 0.8781631934172083 1101 64-65 0.6329739048069101 1101 66-67 0.7487993005923919 1101 68-69 0.7057567229974637 1101 70-71 0.657018576497606 1101 72-73 0.9090896978345882 1101 74-75 0.9336382682943984 1101 76-77 0.8265464320396347 1101 78-79 0.981423904982627 1101 80-81 1.1369154216410173 1101 82-83 1.0611852584936372 1101 84-85 1.081536685339163 1101 86-87 1.1960458761346864 1101 88-89 1.2209261579675825 1101 90-91 1.4092044508290904 1101 92-93 1.4118433886069433 1101 94-95 1.1939272696214864 1101 96-97 1.1951545753520456 1101 98-99 1.4853940966607304 1101 100-101 1.5358914334883345 1102 1 0.3111057664505168 1102 2 0.38638904751007885 1102 3 0.3114611465685755 1102 4 0.20703654385854264 1102 5 0.2941781340165903 1102 6 0.2137747561171892 1102 7 0.19551765049787662 1102 8 0.3082634103601123 1102 9 0.43874187492940564 1102 10-11 0.24302279131935478 1102 12-13 0.2676971655948961 1102 14-15 0.3793608152420873 1102 16-17 0.3638988428193386 1102 18-19 0.43727305050318677 1102 20-21 0.38043390975389 1102 22-23 0.4583456251916189 1102 24-25 0.4600222381462231 1102 26-27 0.43665715401908045 1102 28-29 0.46599972311225457 1102 30-31 0.35789696274736116 1102 32-33 0.5084387442794309 1102 34-35 0.5612444608597755 1102 36-37 0.7650245363059653 1102 38-39 0.698363173905804 1102 40-41 0.6651480998255579 1102 42-43 0.9057749741775112 1102 44-45 0.8698064251741684 1102 46-47 1.0225621292352045 1102 48-49 0.9885476286487815 1102 50-51 0.967004016730364 1102 52-53 0.9759470726739323 1102 54-55 1.074924264819451 1102 56-57 0.9068540802705485 1102 58-59 0.9610195051692401 1102 60-61 1.01805504438547 1102 62-63 0.9712192545748337 1102 64-65 1.089584146282462 1102 66-67 1.1739631156633799 1102 68-69 1.0914016689991684 1102 70-71 0.8251595788031487 1102 72-73 0.8530558427199182 1102 74-75 1.0222864905975193 1102 76-77 1.037588781269097 1102 78-79 1.139905885324822 1102 80-81 1.0459495194446795 1102 82-83 1.2915244163628294 1102 84-85 1.127219854857426 1102 86-87 0.7225930184717804 1102 88-89 0.7386879587199218 1102 90-91 0.981186006465542 1102 92-93 0.857520490900356 1102 94-95 0.9349720457408885 1102 96-97 0.40121876659097566 1102 98-99 0.09473155570696434 1102 100-101 -0.11667500262378994 1103 1 0.1543939156677112 1103 2 0.14704720105082458 1103 3 0.05897206200363314 1103 4 0.02536229575999016 1103 5 0.05908067151270302 1103 6 0.11365266157155673 1103 7 0.18942814697510357 1103 8 0.14877626834680058 1103 9 0.04646823699911096 1103 10-11 0.1663414793967135 1103 12-13 0.14034052773158834 1103 14-15 0.03484258631328885 1103 16-17 0.13827588660895884 1103 18-19 0.10295017284740027 1103 20-21 0.23196115723740007 1103 22-23 0.4045258318693712 1103 24-25 0.2058851816904408 1103 26-27 0.3164473097276854 1103 28-29 0.209134569078266 1103 30-31 0.19785263116170881 1103 32-33 0.23788666989005236 1103 34-35 0.36824373620885353 1103 36-37 0.3243355394298746 1103 38-39 0.4212359580877134 1103 40-41 0.34910134461750886 1103 42-43 0.30665460296137326 1103 44-45 0.2958882254749895 1103 46-47 0.3221999560131863 1103 48-49 -0.21347621784701687 1103 50-51 -0.5736358074047772 1103 52-53 -0.5295327193086266 1103 54-55 -0.25975332641653637 1103 56-57 -0.23604297058289347 1103 58-59 -0.3702144718246103 1103 60-61 -0.33929757440551356 1103 62-63 -0.1403533901574363 1103 64-65 -0.10969409531250562 1103 66-67 -0.1961980125998295 1103 68-69 -0.5318369421068709 1103 70-71 -0.49976262778934455 1103 72-73 0.04153663338105673 1103 74-75 0.09174869609390512 1103 76-77 4.321531489885899E-4 1103 78-79 0.008600965614924405 1103 80-81 0.07507257004236578 1103 82-83 0.2339268472926328 1103 84-85 0.19748587481881685 1103 86-87 0.2148289014802387 1103 88-89 0.4530514229166833 1103 90-91 0.6071557599015449 1103 92-93 0.3287247511170186 1103 94-95 0.41917206766310144 1103 96-97 0.47120466939032113 1103 98-99 0.4796353719988211 1103 100-101 0.18001702682504828 1104 1 0.16741993390854049 1104 2 0.19489155298988692 1104 3 0.18188095628349998 1104 4 0.09941737678872897 1104 5 0.09479091644298876 1104 6 0.12316687117216674 1104 7 0.1035166710016 1104 8 0.15628918783528434 1104 9 0.11543046081152397 1104 10-11 0.1627466510124762 1104 12-13 0.22623804463308517 1104 14-15 0.18934600334717544 1104 16-17 0.17005040792299297 1104 18-19 0.25003397576880815 1104 20-21 0.32615697509942976 1104 22-23 0.29271146107821266 1104 24-25 0.251021473661325 1104 26-27 0.20846139810476672 1104 28-29 0.14626511285023724 1104 30-31 0.15940861218325608 1104 32-33 -0.004045353837426546 1104 34-35 -0.02068980538403764 1104 36-37 -0.1417299565359471 1104 38-39 -0.25089115469130263 1104 40-41 0.11956718089081875 1104 42-43 0.0333528710078852 1104 44-45 -0.20271586732492608 1104 46-47 -0.10692175387027447 1104 48-49 -0.29906443110048997 1104 50-51 -0.28841008921052236 1104 52-53 -0.17238912747777135 1104 54-55 -0.0811396532850992 1104 56-57 -0.2092378789011775 1104 58-59 -0.5116397077696675 1104 60-61 -0.39031651319277927 1104 62-63 -0.46390778131414834 1104 64-65 -0.6579917497475947 1104 66-67 -0.515779945740281 1104 68-69 -0.40969653147505625 1104 70-71 -0.3277060450583349 1104 72-73 -0.30668417268923065 1104 74-75 -0.4740411449343078 1104 76-77 -0.25642285323545266 1104 78-79 -0.07165635151021377 1104 80-81 -0.07903778891149571 1104 82-83 -0.15557681218829345 1104 84-85 -0.3818472048022592 1104 86-87 -0.20491231201862448 1104 88-89 -0.20120366957318936 1104 90-91 -0.18400917608509104 1104 92-93 -0.2709084926333567 1104 94-95 -0.3928374362608693 1104 96-97 -0.2918114305625146 1104 98-99 -0.14559293229719472 1104 100-101 0.20085424788237916 1105 1 0.2285863359532314 1105 2 0.1261350181178713 1105 3 0.1482965547809698 1105 4 0.1703134771643633 1105 5 0.2030955286104401 1105 6 0.20489633715210687 1105 7 0.20744533924309394 1105 8 0.06843146410062673 1105 9 0.1676319865831175 1105 10-11 0.25121913678098196 1105 12-13 0.2602774668535801 1105 14-15 0.36179885545576695 1105 16-17 0.22960487487106462 1105 18-19 0.16790273617620244 1105 20-21 0.20169912744424323 1105 22-23 0.15591480693268522 1105 24-25 0.2522219538534003 1105 26-27 0.38769115452347336 1105 28-29 0.32611447194872056 1105 30-31 0.31718462581450524 1105 32-33 -0.029502310813761312 1105 34-35 0.041338231637226386 1105 36-37 0.17097577091632132 1105 38-39 0.370294416311296 1105 40-41 0.4542784847672081 1105 42-43 0.28655253797144553 1105 44-45 0.1018575749552113 1105 46-47 -0.17635113852736595 1105 48-49 0.10996530982813368 1105 50-51 0.2242288373278356 1105 52-53 0.272204839141132 1105 54-55 0.2779443028780122 1105 56-57 0.28032084781531097 1105 58-59 0.19785376703961077 1105 60-61 0.1126798466414769 1105 62-63 0.09941754881791098 1105 64-65 0.13776203563752176 1105 66-67 0.03163902184675038 1105 68-69 -0.06921517764319773 1105 70-71 -0.020465406867572256 1105 72-73 0.12067225375231416 1105 74-75 0.14582777037375294 1105 76-77 0.1727052496185948 1105 78-79 0.28988181214234743 1105 80-81 0.19326306691470663 1105 82-83 0.12849165390782247 1105 84-85 0.3261359884750483 1105 86-87 0.5053411442025713 1105 88-89 0.5068230895175461 1105 90-91 0.46609441373793814 1105 92-93 0.6733950352294009 1105 94-95 0.5310240438150338 1105 96-97 0.632911684489958 1105 98-99 1.2144010653018462 1105 100-101 1.0093824978920658 1106 1 0.04796277604539512 1106 2 0.05870270244967202 1106 3 0.1643895847348844 1106 4 0.06277459467588642 1106 5 0.09235244105283869 1106 6 -0.013781658239594208 1106 7 0.011304786247698928 1106 8 0.029872996358690784 1106 9 0.08435865709003565 1106 10-11 0.03610536949987164 1106 12-13 0.13470142998722423 1106 14-15 0.13615441421152497 1106 16-17 0.051465473949399154 1106 18-19 0.054585483872052976 1106 20-21 0.07799521039354573 1106 22-23 0.10048925969766742 1106 24-25 0.1400567752819697 1106 26-27 0.06552453585987195 1106 28-29 0.17483278992107643 1106 30-31 0.02950821452418495 1106 32-33 0.04910872029222446 1106 34-35 0.23713676274321926 1106 36-37 0.17698815570894766 1106 38-39 0.2310735061730469 1106 40-41 0.13893430273956398 1106 42-43 0.1671688974184491 1106 44-45 0.40092871550820774 1106 46-47 0.2081714709196376 1106 48-49 0.14531957250095218 1106 50-51 0.12581653894073241 1106 52-53 0.3075591018139434 1106 54-55 0.27537013813147126 1106 56-57 0.3079346150964213 1106 58-59 0.3806734675004435 1106 60-61 0.2601092821253488 1106 62-63 0.3594621916750498 1106 64-65 0.3261440920891374 1106 66-67 0.38041134327532333 1106 68-69 0.4939508649835318 1106 70-71 0.2933126749296626 1106 72-73 0.038965024489641564 1106 74-75 0.13196688327697714 1106 76-77 0.3282532046877158 1106 78-79 0.3045347499303652 1106 80-81 0.49422432843543973 1106 82-83 0.6395443612810929 1106 84-85 0.5409797384750483 1106 86-87 0.4027885808385179 1106 88-89 0.6431278130198521 1106 90-91 0.7659036015259524 1106 92-93 0.5640020340773262 1106 94-95 0.7583245910500551 1106 96-97 0.561969144167378 1106 98-99 0.7367224938732768 1106 100-101 0.9238950266939092 1107 1 -0.04487769417278997 1107 2 -0.034366705497454575 1107 3 -0.0024375871873942856 1107 4 -0.11238640827044577 1107 5 -0.04712538184890036 1107 6 0.046346845503038026 1107 7 0.06922603166894703 1107 8 0.048766580129992576 1107 9 -0.14056892891092332 1107 10-11 0.046528257300479936 1107 12-13 0.04308652315491912 1107 14-15 -0.027022818429024653 1107 16-17 0.20164364722540284 1107 18-19 0.1610371730343445 1107 20-21 0.14703984329687358 1107 22-23 0.07709344129504814 1107 24-25 0.015798956560836075 1107 26-27 -0.022751488387619645 1107 28-29 0.08778419539964943 1107 30-31 0.10574634460063237 1107 32-33 0.12233830999600315 1107 34-35 0.010486544754684246 1107 36-37 0.12850188201887391 1107 38-39 0.21286424713498064 1107 40-41 0.3925686891864473 1107 42-43 0.23273964913050094 1107 44-45 0.28397513014429876 1107 46-47 0.2087195528196233 1107 48-49 0.21687505506186966 1107 50-51 0.2022450357156984 1107 52-53 0.28317972213564957 1107 54-55 0.33938374001871097 1107 56-57 0.48095057106074535 1107 58-59 0.3226103802253988 1107 60-61 0.2593714556402418 1107 62-63 0.27313151601013885 1107 64-65 0.015545892734145639 1107 66-67 0.14598844054399862 1107 68-69 0.473419256646217 1107 70-71 0.43849341131284447 1107 72-73 0.4692516196879204 1107 74-75 0.4495918534155372 1107 76-77 0.2280786396694019 1107 78-79 0.39793014529586657 1107 80-81 0.3706471017671795 1107 82-83 0.34788142729479077 1107 84-85 0.11061600121593429 1107 86-87 0.26865035719621844 1107 88-89 0.35664957205791126 1107 90-91 0.3349127739658364 1107 92-93 0.1542849961333843 1107 94-95 0.38737765981100836 1107 96-97 0.26834841933062137 1107 98-99 0.27888187434787426 1107 100-101 -0.06102307829892695 1108 1 0.12681774708786975 1108 2 0.15814647658094572 1108 3 0.13771366774646765 1108 4 0.15416576262955317 1108 5 0.13858313603353167 1108 6 0.05445917187623195 1108 7 0.08882409126700708 1108 8 0.11363385543205595 1108 9 0.2097194196382972 1108 10-11 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-0.4000342303758124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 19.0 5 36.0 6 106.0 7 199.0 8 490.0 9 951.0 10 1763.0 11 2690.0 12 4383.0 13 6061.0 14 7378.0 15 10498.0 16 14377.0 17 20938.0 18 19040.0 19 9576.0 20 7216.0 21 6941.0 22 7056.0 23 7696.0 24 8480.0 25 9552.0 26 10859.0 27 12491.0 28 14802.0 29 17550.0 30 20647.0 31 25082.0 32 30076.0 33 38312.0 34 51039.0 35 74006.0 36 106513.0 37 190539.0 38 175232.0 39 13654.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.455733898756606 22.989199058301693 16.44809938512385 19.10696765781785 2 14.559997927141172 23.83884818144424 41.66521818458592 19.935935706828666 3 17.009642032394897 30.579633946086236 33.04039807526421 19.370325946254656 4 10.771200649495766 20.539547879260294 40.57869468192953 28.110556789314412 5 9.987832750521724 41.24330504384852 35.461000564638105 13.307861640991646 6 24.443000160862482 41.7523430322254 19.118344044903303 14.686312762008816 7 23.080959172238373 37.962034295017475 23.192267372878153 15.764739159865998 8 24.500975431224813 36.051657369391 21.302295151345685 18.145072048038504 9 26.479475998561952 17.078413442489506 23.320417551500285 33.12169300744826 10-11 22.10018385825964 29.908275996834572 28.607341159095157 19.384198985810634 12-13 23.9960399759462 29.011926495562246 29.361289577298745 17.630743951192812 14-15 21.49953009116933 29.178774264002932 29.834541349008113 19.48715429581962 16-17 17.173171390013493 32.54024291497976 30.699217273954115 19.58736842105263 18-19 16.831622774037214 29.40972613957365 36.26652012698426 17.49213095940487 20-21 18.45475931625888 28.12604924983023 36.64722641772208 16.771965016188812 22-23 18.146799430395667 28.59114694949674 36.15335700566905 17.108696614438543 24-25 17.38550963717413 29.2209937414778 36.77186785093122 16.621628770416848 26-27 17.059907271905782 29.048972935682503 37.04125153237791 16.849868260033805 28-29 16.643059107785422 28.88231883807626 37.1854139833761 17.289208070762218 30-31 17.2222720044221 28.967068534974633 36.7594522902391 17.05120717036416 32-33 16.94432538701462 29.414676488274853 36.57473033941984 17.06626778529069 34-35 17.284717019020864 29.309222388004912 36.58758040520553 16.818480187768692 36-37 17.59322690860289 28.919357651119714 36.059704522106315 17.427710918171073 38-39 16.950954999346816 29.923690742374205 35.87555372255949 17.24980053571949 40-41 17.162360219276582 29.50835512873457 36.31094952374011 17.01833512824873 42-43 17.13670486891022 29.608385168439128 35.56301210952281 17.691897853127838 44-45 17.353381283158036 29.936917406390958 34.99615652861119 17.71354478183981 46-47 17.815180484855684 30.2204036685362 34.03225893517444 17.932156911433676 48-49 17.774895196020957 30.48173663135234 33.45403469678371 18.28933347584299 50-51 17.736708062665112 30.67777085625264 32.89211309604138 18.69340798504087 52-53 17.727560052274992 31.401325012375096 32.56455697159153 18.30655796375838 54-55 17.90593755296856 31.117281704753648 32.25719211838615 18.719588623891642 56-57 17.824253011059625 31.405708165812328 32.00813601482102 18.761902808307028 58-59 17.641108136120227 30.985921575401626 32.3728191397478 19.000151148730353 60-61 17.970639755358285 31.410333575347977 31.982099913360557 18.636926755933185 62-63 17.546948895353452 31.045515421650716 32.62025821420108 18.787277468794755 64-65 17.479607430555145 30.970794806838065 32.333646241618816 19.215951520987975 66-67 17.2442533280576 31.264065398943163 31.859042907800077 19.63263836519916 68-69 17.35892486213136 30.858607451168375 32.025686536161096 19.756781150539165 70-71 17.542215605388137 30.8437615046364 32.139784098797634 19.474238791177825 72-73 17.490286173275884 30.458771161783393 32.010500325503614 20.04044233943711 74-75 17.10392003070427 31.08802052997256 31.312796523205535 20.49526291611764 76-77 16.947403397197647 30.681171247421364 32.09089787409978 20.28052748128121 78-79 16.82084478850598 30.446404906637532 31.907556824223988 20.825193480632503 80-81 16.798379713599694 30.509437995543347 31.58268340501978 21.109498885837176 82-83 16.91328648528432 30.420822730624437 31.548533506359465 21.117357277731774 84-85 16.55631212503657 30.14336193950491 31.42912820092048 21.87119773453804 86-87 16.392205983035762 30.147536014014918 31.649644250321114 21.810613752628203 88-89 16.741962799249983 29.74044640249905 31.561747794886074 21.955843003364894 90-91 16.508343044197648 29.385788057612444 31.780533203578504 22.325335694611404 92-93 16.615259047974806 29.52047865765117 31.507724098171458 22.356538196202568 94-95 16.351941199904562 28.81268373680307 31.783619449029803 23.051755614262564 96-97 16.435557302131052 28.618299240923417 31.408075728442537 23.538067728502995 98-99 16.174245470881985 28.698530050801264 31.18822917841872 23.938995299898032 100-101 16.102898507696338 28.09525480363076 32.08326023411543 23.718586454557478 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1824.0 1 1215.5 2 783.5 3 997.0 4 872.5 5 859.5 6 1500.5 7 2907.0 8 4276.5 9 4608.5 10 3905.5 11 3258.0 12 3297.5 13 3455.0 14 3547.5 15 3692.5 16 3883.5 17 4112.5 18 4390.0 19 4776.5 20 5240.5 21 5873.5 22 6627.5 23 7368.5 24 8286.0 25 9701.5 26 12312.5 27 15360.5 28 16478.5 29 17785.0 30 21171.0 31 24348.0 32 28034.5 33 33073.5 34 37908.5 35 40579.5 36 43421.0 37 46126.5 38 44708.5 39 41794.5 40 38430.5 41 35144.0 42 32204.0 43 30614.5 44 30103.0 45 28870.5 46 26730.5 47 24123.0 48 23860.0 49 22186.0 50 18539.5 51 15307.5 52 12052.0 53 9979.0 54 8909.5 55 8112.0 56 7168.0 57 6076.0 58 4892.0 59 3899.0 60 3192.0 61 2707.5 62 2298.0 63 1765.0 64 1282.5 65 958.0 66 742.0 67 596.0 68 469.5 69 335.0 70 249.0 71 196.0 72 165.0 73 138.0 74 112.5 75 115.5 76 81.0 77 47.5 78 45.5 79 41.0 80 30.0 81 14.5 82 7.0 83 4.5 84 3.5 85 3.0 86 3.0 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3043431790528978 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014574788638574393 16-17 7.557297812594129E-4 18-19 5.398069866138663E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.398069866138663E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001187575370550506 36-37 0.006747587332673329 38-39 0.0028069963303921047 40-41 0.0031848612210218113 42-43 2.6990349330693317E-4 44-45 0.001187575370550506 46-47 2.1592279464554653E-4 48-49 4.3184558929109306E-4 50-51 0.0 52-53 1.619420959841599E-4 54-55 0.0 56-57 5.398069866138663E-4 58-59 0.0018353437544871456 60-61 4.8582628795247975E-4 62-63 0.0011335946718891193 64-65 5.398069866138663E-5 66-67 3.7786489062970643E-4 68-69 0.0014034981651960524 70-71 0.0 72-73 0.0 74-75 1.619420959841599E-4 76-77 0.2503624803915112 78-79 1.619420959841599E-4 80-81 1.0796139732277327E-4 82-83 0.0 84-85 0.0 86-87 0.1033730379365554 88-89 0.361184854743338 90-91 0.2659629023046519 92-93 0.0 94-95 0.0 96-97 0.0 98-99 5.398069866138663E-5 100-101 4.8582628795247975E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 926257.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.675049313837306 #Duplication Level Percentage of deduplicated Percentage of total 1 72.70210262938444 27.390553017817197 2 13.003858982534966 9.79842056874382 3 5.146577704269059 5.816927064174972 4 2.5968466081732604 3.9134529609359494 5 1.5238828179211155 2.8706180156843693 6 0.9627669219373568 2.176337475703276 7 0.6790762717717315 1.7908962418799739 8 0.45168819458442366 1.361390000435704 9 0.33339091543820876 1.130446726192789 >10 2.0994628101773993 15.560036711672746 >50 0.2916059648334512 7.77507777648994 >100 0.19641074532656183 13.750636073429428 >500 0.009748854512445582 2.3309861355213934 >1k 0.0017203860904315732 1.1638726769908003 >5k 2.8673101507192887E-4 0.7716300846245858 >10k+ 5.734620301438577E-4 2.398718469703056 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11990 1.2944571539000516 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10215 1.1028256736521291 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7143 0.7711682610765696 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2963 0.3198896202673772 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2264 0.24442460353875867 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2055 0.22186067149829905 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1318 0.14229312167141517 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1119 0.12080880360418329 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1055 0.11389927417552581 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 975 0.10526236238970395 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 940 0.10148371348340689 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 937 0.10115982929143855 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 928 0.10018817671553361 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.398069866138663E-5 0.0 20-21 0.0 0.0 0.0 3.7786489062970643E-4 0.0 22-23 0.0 0.0 0.0 0.0016734016585029858 0.0 24-25 0.0 0.0 0.0 0.004858262879524797 0.0 26-27 0.0 0.0 0.0 0.008420988991176315 0.0 28-29 0.0 0.0 0.0 0.01765168846227343 0.0 30-31 0.0 0.0 0.0 0.04377834661438456 0.0 32-33 0.0 0.0 0.0 0.08901417209262656 0.0 34-35 0.0 0.0 0.0 0.16178015388817574 0.0 36-37 0.0 0.0 0.0 0.2758953508583471 0.0 38-39 0.0 0.0 0.0 0.44431513068187334 0.0 40-41 0.0 0.0 0.0 0.6250425098001958 0.0 42-43 0.0 0.0 0.0 0.8053380433292272 0.0 44-45 0.0 0.0 0.0 1.020181224001546 0.0 46-47 0.0 0.0 0.0 1.2723790481475443 0.0 48-49 0.0 0.0 0.0 1.5476266306219548 0.0 50-51 0.0 0.0 0.0 1.828002379469197 0.0 52-53 0.0 0.0 0.0 2.103195981244946 0.0 54-55 0.0 0.0 0.0 2.4108319829161884 0.0 56-57 0.0 0.0 0.0 2.7749317953872414 0.0 58-59 0.0 0.0 0.0 3.178761402073075 0.0 60-61 0.0 0.0 0.0 3.615627196339677 0.0 62-63 0.0 0.0 0.0 4.04164287017534 0.0 64-65 0.0 0.0 0.0 4.486497807843827 0.0 66-67 0.0 0.0 0.0 4.993376568274248 0.0 68-69 0.0 0.0 0.0 5.551968838022277 0.0 70-71 0.0 0.0 0.0 6.174042409396097 0.0 72-73 0.0 0.0 0.0 6.794118694919445 0.0 74-75 0.0 0.0 0.0 7.43432978104349 0.0 76-77 0.0 0.0 0.0 8.114216680683654 0.0 78-79 0.0 0.0 0.0 8.878799296523535 0.0 80-81 0.0 0.0 0.0 9.70459602464543 0.0 82-83 0.0 0.0 0.0 10.536060726126767 0.0 84-85 0.0 0.0 0.0 11.357485017657087 0.0 86-87 0.0 0.0 0.0 12.22069036995132 0.0 88-89 0.0 0.0 0.0 13.132910196630093 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5925 0.0 47.401676 1 GTATCAA 9890 0.0 37.735474 1 TATCAAC 12655 0.0 29.362381 2 ATCAACG 12870 0.0 28.834993 3 TCAACGC 12925 0.0 28.74901 4 ATGGGCG 100 4.9834227E-5 28.473637 5 CAACGCA 13065 0.0 28.440945 5 AACGCAG 13275 0.0 27.919535 6 CGCCGAA 70 0.008428706 27.11775 7 GTACATG 7735 0.0 26.647572 1 ACATGGG 7585 0.0 25.651924 3 TACATGG 7915 0.0 25.601688 2 CATGGGT 760 0.0 24.976873 4 ACGCAGA 14865 0.0 24.773563 7 CGCAGAG 14855 0.0 24.63051 8 GAGTACT 9410 0.0 24.20713 12-13 CATGGGG 2375 0.0 24.177614 4 GCAGAGT 15295 0.0 23.735788 9 CATGGGA 4305 0.0 23.369768 4 CTCCGAC 185 0.0 23.086733 94-95 >>END_MODULE