FastQCFastQC Report
Fri 27 May 2016
SRR939348_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939348_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945812
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127121.3440303146925605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100241.0598300719381863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70510.7454969909453465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30640.32395444337775375No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21920.23175853129374546No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19730.20860382401576635No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.14432043577370554No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11960.1264521913445801No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10590.11196728313872101No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9870.10435477663637172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57200.048.4033851
GTATCAA95350.037.4043771
TATCAAC122400.029.0592772
ATCAACG122600.028.9345073
TCAACGC123050.028.828694
CAACGCA124600.028.4320055
AACGCAG126900.027.972186
GATTACG700.00848440527.0812116
GTACATG75700.025.8466641
GAGTACT87850.024.97729112-13
ACGCAGA142450.024.719097
TACATGG76000.024.6483822
CGCAGAG142850.024.583528
CATTCCG2552.0008883E-1124.159419
CATGGGG22800.024.1283744
ACATGGG73450.024.0190643
GCAGAGT145750.023.3452769
GTACTTT96850.022.75529314-15
CTGCTCG1252.2913027E-422.7470119
CTGCGGA1050.002225827222.566489
Produced by FastQC (version 0.11.5)