##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939348_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 945812 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.008287059161866 33.0 31.0 34.0 30.0 34.0 2 32.0661251918986 33.0 31.0 34.0 30.0 34.0 3 32.06424638300212 34.0 31.0 34.0 30.0 34.0 4 35.61557793726449 37.0 35.0 37.0 33.0 37.0 5 35.68979776107725 37.0 35.0 37.0 35.0 37.0 6 35.810765775862436 37.0 35.0 37.0 35.0 37.0 7 35.75807454335534 37.0 35.0 37.0 35.0 37.0 8 35.75540487961667 37.0 35.0 37.0 35.0 37.0 9 37.383614291212204 39.0 37.0 39.0 35.0 39.0 10-11 37.31980298410255 39.0 37.0 39.0 34.5 39.0 12-13 37.238880982690006 39.0 37.0 39.0 34.0 39.0 14-15 38.4111789657987 40.0 38.0 41.0 34.0 41.0 16-17 38.38730159904928 40.0 38.0 41.0 34.0 41.0 18-19 38.4796894097347 40.0 38.0 41.0 34.0 41.0 20-21 38.46435443830275 40.0 38.0 41.0 34.0 41.0 22-23 38.36810328056738 40.0 38.0 41.0 34.0 41.0 24-25 38.350241379893674 40.0 38.0 41.0 34.0 41.0 26-27 38.24466225846152 40.0 38.0 41.0 34.0 41.0 28-29 38.09751197912482 40.0 38.0 41.0 34.0 41.0 30-31 37.7916874600872 40.0 37.0 41.0 33.0 41.0 32-33 37.43415181875468 40.0 36.0 41.0 33.0 41.0 34-35 37.07906433836746 40.0 35.0 41.0 32.5 41.0 36-37 36.78196354032302 40.0 35.0 41.0 31.0 41.0 38-39 36.43864795540763 39.0 35.0 41.0 30.5 41.0 40-41 36.242021670268514 39.0 35.0 41.0 30.0 41.0 42-43 35.91678155912591 39.0 35.0 41.0 30.0 41.0 44-45 35.48531209162074 39.0 35.0 41.0 26.5 41.0 46-47 34.929673127429126 39.0 35.0 41.0 22.0 41.0 48-49 34.48808537003126 39.0 35.0 41.0 12.5 41.0 50-51 33.29221293449438 38.0 34.0 40.0 7.5 40.5 52-53 33.38560623041366 38.5 33.5 39.5 3.5 40.5 54-55 33.86873871340181 39.0 35.0 40.5 2.0 41.0 56-57 33.78937991905368 39.0 35.0 41.0 2.0 41.0 58-59 33.62192962237739 39.0 34.5 41.0 2.0 41.0 60-61 33.3708517126025 39.0 34.0 41.0 2.0 41.0 62-63 33.03712577129493 38.0 34.0 40.0 2.0 41.0 64-65 32.70550489949377 38.0 33.5 40.0 2.0 41.0 66-67 32.35659359365286 37.5 33.0 40.0 2.0 41.0 68-69 31.946918097888375 37.0 33.0 39.5 2.0 41.0 70-71 31.54305242479478 36.0 32.0 39.0 2.0 41.0 72-73 31.089151438129353 36.0 31.5 39.0 2.0 40.5 74-75 30.61037447188236 35.0 31.0 38.5 2.0 40.0 76-77 30.144174529399077 35.0 31.0 37.0 2.0 39.5 78-79 29.67859310306911 35.0 30.5 37.0 2.0 39.0 80-81 29.174064190346495 35.0 29.5 36.5 2.0 39.0 82-83 28.67515002981565 35.0 29.0 36.0 2.0 37.5 84-85 28.19177066901245 34.0 29.0 35.5 2.0 37.0 86-87 27.842447547715615 34.0 28.5 35.0 2.0 37.0 88-89 27.389579535890853 34.0 26.5 35.0 2.0 36.0 90-91 26.95085122624792 34.0 26.0 35.0 2.0 36.0 92-93 26.514604382266242 34.0 25.0 35.0 2.0 36.0 94-95 26.039082291195292 34.0 23.0 35.0 2.0 35.0 96-97 25.497690344381336 34.0 18.5 35.0 2.0 35.0 98-99 24.861868426283447 33.0 2.0 35.0 2.0 35.0 100-101 22.753064033867197 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08915383540222876 1101 2 0.029111794667237234 1101 3 0.2968575847862951 1101 4 0.2365987109770984 1101 5 0.1388176854787062 1101 6 0.2494393560728696 1101 7 0.3625858169220564 1101 8 0.3058839495723191 1101 9 0.4881901917558551 1101 10-11 0.435561680493592 1101 12-13 0.407064479637846 1101 14-15 0.4239276704124393 1101 16-17 0.5522972340247989 1101 18-19 0.5036918633016327 1101 20-21 0.5741831159923194 1101 22-23 0.422010466304819 1101 24-25 0.3729849307026498 1101 26-27 0.4037285928179273 1101 28-29 0.34797588734488016 1101 30-31 0.39984410423920025 1101 32-33 0.5807154777695587 1101 34-35 0.6294691330111775 1101 36-37 0.5159608447176041 1101 38-39 0.7498239201968246 1101 40-41 0.67795202442651 1101 42-43 0.5597921626737161 1101 44-45 0.5932131693003981 1101 46-47 0.5282961746713681 1101 48-49 0.5735817311327267 1101 50-51 0.6758827721425149 1101 52-53 0.5968951956783215 1101 54-55 0.8201362958804026 1101 56-57 0.8295553464344323 1101 58-59 0.8075941933542339 1101 60-61 0.8853928984930732 1101 62-63 1.1030788502043691 1101 64-65 1.1378071825845737 1101 66-67 1.195079412893712 1101 68-69 1.0687698488745312 1101 70-71 0.9565869960317599 1101 72-73 1.0981750179218146 1101 74-75 0.807543280435528 1101 76-77 0.6728260712331817 1101 78-79 0.7403629478714464 1101 80-81 0.8000509726929756 1101 82-83 0.81024621422225 1101 84-85 0.7333890368054412 1101 86-87 0.8363065259105262 1101 88-89 0.5919613528814018 1101 90-91 0.3594573842070439 1101 92-93 0.531442579415959 1101 94-95 0.3747280900294001 1101 96-97 0.3743161748083672 1101 98-99 0.2830521953394509 1101 100-101 0.22513942803412235 1102 1 0.20519411810917632 1102 2 0.36633083188627324 1102 3 0.3993267205887676 1102 4 0.2826841903959121 1102 5 0.2918919718863222 1102 6 0.2601136667471806 1102 7 0.21691893792184658 1102 8 0.28945236647407313 1102 9 0.4296225331881942 1102 10-11 0.4119865902518356 1102 12-13 0.31518775442778946 1102 14-15 0.4920837052351317 1102 16-17 0.3785765436374433 1102 18-19 0.5004598434029504 1102 20-21 0.49201761716015824 1102 22-23 0.5388894665171549 1102 24-25 0.6571706315550117 1102 26-27 0.7103943494836784 1102 28-29 0.6501234894924863 1102 30-31 0.644297648692735 1102 32-33 0.6147616451490592 1102 34-35 0.6649986018739824 1102 36-37 0.6566651854219501 1102 38-39 0.6324292528021616 1102 40-41 0.5533016164427664 1102 42-43 0.4889076417891971 1102 44-45 0.6162104089643066 1102 46-47 0.4720869351287931 1102 48-49 0.44135253223686277 1102 50-51 0.40223184849158855 1102 52-53 0.5811081965579916 1102 54-55 0.5472694896802608 1102 56-57 0.6270589106046671 1102 58-59 0.3499410857011256 1102 60-61 0.25349129992480357 1102 62-63 0.35566325278876576 1102 64-65 0.4237279518386785 1102 66-67 0.540885825366793 1102 68-69 0.42641385651852914 1102 70-71 0.29521956799766613 1102 72-73 0.39595613236958727 1102 74-75 0.2087126316048824 1102 76-77 0.16350766191477462 1102 78-79 0.17712243463093458 1102 80-81 0.014823320798658557 1102 82-83 -0.2932057225630196 1102 84-85 -0.03743178401537506 1102 86-87 0.019967459571457624 1102 88-89 0.017427728347779947 1102 90-91 0.19417998559631044 1102 92-93 0.27410645541316825 1102 94-95 0.2875712362058813 1102 96-97 0.27094158810044533 1102 98-99 0.10106263001655336 1102 100-101 -0.4111787082840124 1103 1 0.26011465129057854 1103 2 0.33676074796836275 1103 3 0.3268629524728226 1103 4 0.3432546422707148 1103 5 0.4259083993810151 1103 6 0.3045118198409895 1103 7 0.21212956356725243 1103 8 0.3473224895615843 1103 9 0.26170065874458714 1103 10-11 0.4387554539772154 1103 12-13 0.4830977592943171 1103 14-15 0.5551541867838807 1103 16-17 0.42658601555996256 1103 18-19 0.36409175594790355 1103 20-21 0.5503640070282785 1103 22-23 0.6277807293214579 1103 24-25 0.5266349575410771 1103 26-27 0.5312245670530302 1103 28-29 0.542742392980955 1103 30-31 0.7119616565741396 1103 32-33 0.7059704428795968 1103 34-35 0.5481218436928756 1103 36-37 0.5091975596934546 1103 38-39 0.7446709411307992 1103 40-41 0.8067630818607512 1103 42-43 0.7410723559104326 1103 44-45 1.1058809095043713 1103 46-47 1.0202714833133442 1103 48-49 0.7627658427806026 1103 50-51 0.4637652198075699 1103 52-53 0.5297722756568461 1103 54-55 0.6625141810119075 1103 56-57 0.7272069835788244 1103 58-59 0.5610563511642184 1103 60-61 0.5313402951651014 1103 62-63 0.4665651626037217 1103 64-65 0.5835532910118459 1103 66-67 0.5165635782184523 1103 68-69 0.5697360324494056 1103 70-71 0.6702477582432493 1103 72-73 0.47199412688584275 1103 74-75 0.4720762917076762 1103 76-77 0.4033413691397847 1103 78-79 0.29000062903086743 1103 80-81 0.11673105857596155 1103 82-83 0.09775292383040934 1103 84-85 0.07726987416454278 1103 86-87 -0.018765937857590842 1103 88-89 -0.22659742328606924 1103 90-91 -0.076425600226667 1103 92-93 0.06692298736013313 1103 94-95 0.08154505191882677 1103 96-97 0.08456845607513941 1103 98-99 -0.044698744005689406 1103 100-101 0.020630571351350824 1104 1 0.2300219407095696 1104 2 0.22557742794412405 1104 3 0.1900652903425808 1104 4 0.35795977505016197 1104 5 0.37618760717844424 1104 6 0.23496755719310158 1104 7 0.23696093451717815 1104 8 0.3468750774723972 1104 9 0.3609964094624232 1104 10-11 0.3578432430252505 1104 12-13 0.3074545336230514 1104 14-15 0.397725305484002 1104 16-17 0.5616549113582536 1104 18-19 0.3748445126293518 1104 20-21 0.5271559207627021 1104 22-23 0.5244426953294692 1104 24-25 0.5223307399742012 1104 26-27 0.5152217976288256 1104 28-29 0.3637350814745375 1104 30-31 0.4550501308989183 1104 32-33 0.606414173730073 1104 34-35 0.5273596573768842 1104 36-37 0.646363345694553 1104 38-39 0.6508942167536418 1104 40-41 0.5388800489731409 1104 42-43 0.7237429246901286 1104 44-45 0.6660295182793163 1104 46-47 0.8328284717202692 1104 48-49 0.7461238120960658 1104 50-51 0.8450417318055727 1104 52-53 1.151679327211184 1104 54-55 0.9749182564263279 1104 56-57 0.9327850513249487 1104 58-59 1.1829534916912579 1104 60-61 1.1700295476471112 1104 62-63 1.2371524900810513 1104 64-65 1.3899463283735898 1104 66-67 1.3896620768394072 1104 68-69 1.5083230597642014 1104 70-71 1.2727702562752015 1104 72-73 1.2852894868260414 1104 74-75 1.2454769350749295 1104 76-77 1.2167407948665456 1104 78-79 1.2635034975653348 1104 80-81 1.2679471416247061 1104 82-83 1.2829822366847274 1104 84-85 1.0189194089126055 1104 86-87 0.8518399373993866 1104 88-89 1.1578095574752112 1104 90-91 1.1783203659852255 1104 92-93 1.2541040646604813 1104 94-95 1.057124280671001 1104 96-97 0.8512161502038467 1104 98-99 0.8242307517778755 1104 100-101 0.7877398361796644 1105 1 0.08529581071086767 1105 2 0.13929697985242484 1105 3 0.27316931318136284 1105 4 0.0710122912240152 1105 5 0.22809237683672734 1105 6 0.11263380051731531 1105 7 0.22747779223070097 1105 8 0.1993253075970145 1105 9 0.25948648805215413 1105 10-11 0.2335169274071731 1105 12-13 0.2107296030946415 1105 14-15 0.26995390498034055 1105 16-17 0.21923396241986381 1105 18-19 0.16368414725224767 1105 20-21 0.3025781777207186 1105 22-23 0.26533608358877103 1105 24-25 0.26264542452980066 1105 26-27 0.2743304446697721 1105 28-29 0.2840869984559973 1105 30-31 0.32669595609105073 1105 32-33 0.30251331727572506 1105 34-35 0.29895215770253714 1105 36-37 0.2291552891620583 1105 38-39 0.24947669797460748 1105 40-41 0.2901819626981137 1105 42-43 0.27136623674779514 1105 44-45 0.4762764409053375 1105 46-47 0.29168352035038225 1105 48-49 0.42709253360186494 1105 50-51 0.3310911054758421 1105 52-53 0.5098195783943709 1105 54-55 0.4861856785964491 1105 56-57 0.5601880624838174 1105 58-59 0.4136127118727515 1105 60-61 0.6950379602214625 1105 62-63 0.8176621835376991 1105 64-65 0.5969892813500124 1105 66-67 0.636128795609757 1105 68-69 0.393885589615266 1105 70-71 0.19007465035274862 1105 72-73 0.34477995619340973 1105 74-75 0.5543025396947918 1105 76-77 0.668890886047997 1105 78-79 0.4860805404640409 1105 80-81 0.6468488121991491 1105 82-83 0.5031474487901519 1105 84-85 0.5357038516202586 1105 86-87 0.41616763702163695 1105 88-89 0.41568820473325374 1105 90-91 0.476664174330498 1105 92-93 0.5240737522554646 1105 94-95 0.3142342628689079 1105 96-97 0.17034240937626777 1105 98-99 0.20998120768513218 1105 100-101 -0.044536497891801474 1106 1 0.21733346917470442 1106 2 0.2555348620299043 1106 3 0.23937146331807213 1106 4 0.18378571901422447 1106 5 0.24245510365751244 1106 6 0.22199714876489196 1106 7 0.20371598512157618 1106 8 0.2512066772216741 1106 9 0.15356429068700095 1106 10-11 0.2297534113743609 1106 12-13 0.29460280095693037 1106 14-15 0.2785800856912459 1106 16-17 0.3055617961260495 1106 18-19 0.2371950532925169 1106 20-21 0.3118374369799781 1106 22-23 0.3900525576131244 1106 24-25 0.30460726892453494 1106 26-27 0.20366645013832851 1106 28-29 0.199106884113462 1106 30-31 0.2199659038912003 1106 32-33 -0.05464784976379633 1106 34-35 -0.059187756540573844 1106 36-37 -0.05764094499156158 1106 38-39 0.19250351254084563 1106 40-41 0.2050503755740749 1106 42-43 0.0612463013761797 1106 44-45 0.23484047893666116 1106 46-47 0.18283673312691207 1106 48-49 0.30587935313952386 1106 50-51 0.21638737070080794 1106 52-53 0.099285617419973 1106 54-55 0.18659582028175947 1106 56-57 0.3088908340473324 1106 58-59 0.3253545711817267 1106 60-61 0.3929701625580364 1106 62-63 0.342092024452441 1106 64-65 0.3822474841837149 1106 66-67 0.37373133103108813 1106 68-69 0.4709696997321018 1106 70-71 0.5819619235319706 1106 72-73 0.40943941430924724 1106 74-75 0.22430937538954865 1106 76-77 0.35930382415388706 1106 78-79 0.3068804721070926 1106 80-81 0.3155079285302449 1106 82-83 0.4932356913951743 1106 84-85 0.5349426037952512 1106 86-87 0.35755932233110954 1106 88-89 0.5991450466422812 1106 90-91 0.6360213083101165 1106 92-93 0.7513489511120355 1106 94-95 0.49233518258056463 1106 96-97 0.5386504195676665 1106 98-99 0.3770906409438908 1106 100-101 0.5828326917676527 1107 1 0.06819426984172594 1107 2 0.11553689799374922 1107 3 0.1095109026039367 1107 4 0.22739897313886814 1107 5 0.21792901059941983 1107 6 0.1391588697360504 1107 7 0.20962512781112963 1107 8 0.11563208701998917 1107 9 0.26739648363820123 1107 10-11 0.25666570253657994 1107 12-13 0.25457126775496164 1107 14-15 0.2278627720001225 1107 16-17 0.36441084637315413 1107 18-19 0.2142044163024508 1107 20-21 0.4752538327907416 1107 22-23 0.4052889596772147 1107 24-25 0.3007934723609509 1107 26-27 0.3693920894673397 1107 28-29 0.2402133577513652 1107 30-31 0.49446109392660986 1107 32-33 0.44085485152395165 1107 34-35 0.5119370650096258 1107 36-37 0.3800634088201704 1107 38-39 0.537555150885801 1107 40-41 0.35831664328878077 1107 42-43 0.5417531594234504 1107 44-45 0.7751828601902844 1107 46-47 0.6846509223406727 1107 48-49 0.7117978273300452 1107 50-51 0.683342344390816 1107 52-53 0.6647121639647722 1107 54-55 0.5628679947153827 1107 56-57 0.7509997775032815 1107 58-59 0.8614002203996947 1107 60-61 0.7183905042606327 1107 62-63 0.64877725717038 1107 64-65 0.5481712664228411 1107 66-67 0.5070960521403904 1107 68-69 0.6119202039967107 1107 70-71 0.6326707540404044 1107 72-73 0.587070594610811 1107 74-75 0.6569092769846243 1107 76-77 0.7851971638859645 1107 78-79 0.7979228371214795 1107 80-81 0.7432227287755317 1107 82-83 0.5416340665021231 1107 84-85 0.6602198220869369 1107 86-87 0.5380941748812731 1107 88-89 0.48707190221730556 1107 90-91 0.6764818481798649 1107 92-93 0.6172249851514167 1107 94-95 0.5854914355345322 1107 96-97 0.8109084731377543 1107 98-99 0.8772384906994546 1107 100-101 0.9825026959607719 1108 1 -0.08198053951516115 1108 2 -0.03364496637646397 1108 3 -0.09424929654185377 1108 4 -0.0664837433544605 1108 5 0.04746494801604939 1108 6 -0.0381885287712862 1108 7 0.07162433438313087 1108 8 -0.027771904711656248 1108 9 -0.04274802697679547 1108 10-11 0.08182096618899237 1108 12-13 -0.006123190544826684 1108 14-15 0.020126152977795186 1108 16-17 0.09280269308843714 1108 18-19 -0.061144100427974024 1108 20-21 0.012230013710883725 1108 22-23 0.19055938638835102 1108 24-25 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-0.9694969914676399 2316 68-69 -1.067086975691975 2316 70-71 -0.8378746699352817 2316 72-73 -1.0253603453985 2316 74-75 -0.7753961105683764 2316 76-77 -0.5246908004772344 2316 78-79 -0.6477170676279229 2316 80-81 -0.6548485504861183 2316 82-83 -0.5042107158260762 2316 84-85 -0.21833849718015585 2316 86-87 -0.26809626397613684 2316 88-89 -0.5059930531375194 2316 90-91 -0.5981636777150925 2316 92-93 -0.5149678094675494 2316 94-95 -0.6169227005461231 2316 96-97 -0.5949536326334872 2316 98-99 -0.7320989773386017 2316 100-101 -0.5977721932645963 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3630.0 3 695.0 4 585.0 5 641.0 6 685.0 7 819.0 8 1087.0 9 1389.0 10 1845.0 11 3017.0 12 4771.0 13 7702.0 14 7961.0 15 10283.0 16 14503.0 17 21597.0 18 16161.0 19 8284.0 20 6899.0 21 6713.0 22 6692.0 23 7205.0 24 7965.0 25 8847.0 26 9755.0 27 11481.0 28 13249.0 29 15531.0 30 18176.0 31 21830.0 32 26839.0 33 34444.0 34 46095.0 35 67639.0 36 95577.0 37 167965.0 38 218569.0 39 48668.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.499443295689524 22.299984094162557 16.041673294098935 19.15889931604899 2 14.40168176280243 23.3864317900591 42.18326430075271 20.028622146385764 3 17.15196138109177 30.509996135991912 32.660925350539635 19.677117132376683 4 10.72690990509361 19.772288180899096 41.24124643373226 28.259555480275033 5 10.097561233834638 41.04887590189882 35.43881380785254 13.414749056414003 6 24.56637950825135 41.300136790801055 19.623820007426694 14.509663693520897 7 23.308335907842448 37.641192427980755 23.51179266243742 15.53867900173938 8 24.42285289851162 35.62488691948032 21.73304427042829 18.219215911579763 9 26.915438729766418 17.0207025558933 23.576282681355003 32.487576032985274 10-11 22.093998827738996 29.66381077929965 28.96156507423967 19.280625318721686 12-13 24.113317223049837 28.8157883599675 29.52128334913326 17.549611067849405 14-15 21.61967863519143 28.974304040203663 29.96114527540832 19.444872049196587 16-17 17.333719151313296 32.29751433063402 30.81763435035517 19.551132167697514 18-19 17.00626577818595 29.4402698453341 36.21793311847844 17.33553125800151 20-21 18.736777171903245 28.105430280547477 36.466093216738784 16.69169933081049 22-23 18.36376209430156 28.600724957051245 35.97595398623206 17.059558962415142 24-25 17.563714943949474 29.15659118832617 36.49945870209143 16.780235165632927 26-27 17.27208318275305 29.025493793093958 36.72432193896961 16.978101085183376 28-29 16.85482313538853 28.9526218184608 36.95921439361272 17.233340652537958 30-31 17.426435303224057 29.08446296461879 36.479476167826505 17.00962556433065 32-33 17.160308008919667 29.415751201589245 36.26670123081888 17.157239558672206 34-35 17.476170111514392 29.37656761636463 36.21067280120359 16.936589470917387 36-37 17.789889561333414 28.988956133341397 35.72383647818765 17.49731782713754 38-39 17.10743661368162 30.025737513433686 35.55935594684026 17.307469926044433 40-41 17.300867941840206 29.514530667013172 36.11083561611142 17.073765775035202 42-43 17.20136801213323 29.754582188151 35.27819376564596 17.765856034069806 44-45 17.438506912501076 30.031259848284687 34.744801432053336 17.785431807160904 46-47 17.904797378463158 30.30865455224609 33.79149740860065 17.995050660690108 48-49 17.776152856724416 30.612097619785455 33.3828061655495 18.228943357940626 50-51 17.781280974282456 30.830222197696312 32.81910276192437 18.569394066096866 52-53 17.861304309991702 31.337005821789155 32.64987976509091 18.15181010312823 54-55 17.982226009064725 31.128782649800595 32.34423883846741 18.544752502667265 56-57 17.84061639388783 31.453072421826267 32.25300325467515 18.453307929610755 58-59 17.78875934458502 30.831402896927422 32.626602686488646 18.753235071998915 60-61 18.028493804254193 31.192207275859786 32.33789675965807 18.441402160227952 62-63 17.718952497407237 30.874112426427875 32.90137423046012 18.505560845704768 64-65 17.73093676888308 30.842050356295548 32.57447078344692 18.85254209137446 66-67 17.515183515570897 31.096264417374876 32.13057425395806 19.257977813096165 68-69 17.679266851991606 30.801302157135595 32.24165692808522 19.277774062787582 70-71 17.802080226385694 30.8613971419443 32.34408120504103 18.99244142662898 72-73 17.916208768612858 30.394260173827032 32.26890497671286 19.420626080847242 74-75 17.675236806495263 31.09756162275465 31.69741834487516 19.529783225874926 76-77 17.649733569405925 30.812248538385585 32.12400071946607 19.414017172742422 78-79 17.696559926117793 30.5541748909776 31.950499771107328 19.79876541179728 80-81 17.7970920702953 30.618133981190226 31.57214922633793 20.012624722176543 82-83 18.011951485288236 30.54085139478205 31.643212513408812 19.803984606520903 84-85 17.842086330553556 30.22577437842277 31.508986089785555 20.423153201238115 86-87 17.830870455116976 30.239235363585337 31.760942893127677 20.168951288170014 88-89 18.360175058420015 29.73138465522108 31.72583661072636 20.18260367563254 90-91 18.410972950280676 29.459817630534385 31.892525038517732 20.236684380667203 92-93 18.70346410175036 29.600148171892133 31.656776157486767 20.03961156887074 94-95 18.745170548625676 28.849444241595695 31.758472237553814 20.64691297222482 96-97 19.016154353204918 28.681221400298696 31.44165315959363 20.860971086902758 98-99 18.979448231066243 28.69165554029586 31.215678949189456 21.113217279448442 100-101 19.43121273441703 28.11131473029772 31.738100215303415 20.719372319981833 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2674.0 1 1657.0 2 861.0 3 1092.5 4 922.5 5 926.5 6 1636.0 7 3143.0 8 4797.0 9 5272.0 10 4473.5 11 3823.0 12 3766.5 13 3792.5 14 4007.5 15 4142.0 16 4174.0 17 4339.5 18 4604.5 19 5056.0 20 5470.0 21 5893.0 22 6612.5 23 7574.0 24 8446.0 25 9877.0 26 12614.5 27 15605.5 28 16759.5 29 18050.0 30 21393.5 31 24376.0 32 28173.0 33 33441.5 34 38478.5 35 41316.5 36 44029.0 37 46644.5 38 45017.5 39 41912.0 40 38588.5 41 35481.5 42 32672.5 43 30902.5 44 30160.5 45 29087.5 46 26952.5 47 24060.0 48 23664.0 49 22228.5 50 18576.5 51 15388.0 52 12548.0 53 10525.0 54 9450.5 55 8723.0 56 7638.0 57 6513.5 58 5289.5 59 4146.0 60 3469.0 61 2985.0 62 2489.0 63 1882.5 64 1413.0 65 1132.0 66 890.5 67 711.5 68 567.0 69 419.0 70 322.0 71 263.0 72 217.0 73 188.5 74 178.5 75 156.0 76 99.5 77 70.0 78 63.5 79 49.5 80 40.5 81 33.0 82 23.0 83 12.5 84 7.0 85 6.0 86 4.5 87 1.5 88 3.0 89 2.0 90 1.0 91 1.0 92 1.0 93 1.5 94 2.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29202420777067745 2 0.11513916084803322 3 0.12655792060155718 4 0.12655792060155718 5 0.13501626115972307 6 0.060582864247863216 7 0.06851255852114373 8 0.07284745805720376 9 0.07009849737579983 10-11 0.0666094318955564 12-13 0.06978130960486861 14-15 0.0660279209821825 16-17 0.0670323489234647 18-19 0.07168443623045595 20-21 0.07543782485314206 22-23 0.08357897764037674 24-25 0.09103289025726043 26-27 0.09055710860086359 28-29 0.08558783352294114 30-31 0.08209876804269771 32-33 0.07501490782523376 34-35 0.24333588493273503 36-37 0.3678320850232393 38-39 0.34034247820920016 40-41 0.3240073080062422 42-43 0.3464219104853819 44-45 0.35831645189530265 46-47 0.3662461461685832 48-49 0.37470448672674905 50-51 0.36984094090580366 52-53 0.3689951068499871 54-55 0.3560432728702956 56-57 0.3349502861033694 58-59 0.31930235607076246 60-61 0.27748643493632985 62-63 0.34726774454119846 64-65 0.3749688098691918 66-67 0.3795151679192059 68-69 0.38940085344656233 70-71 0.3915154385861038 72-73 0.3757089146680313 74-75 0.37925084477676324 76-77 0.3643958841714844 78-79 0.3415583646644365 80-81 0.340131019695246 82-83 0.35260707201854063 84-85 0.3606424955487983 86-87 0.3542987401301738 88-89 0.36984094090580366 90-91 0.39262559578436307 92-93 0.38728626830702084 94-95 0.3886607486477228 96-97 0.39257273115587454 98-99 0.3812597006593276 100-101 0.361699788118569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 945812.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.40162769454539 #Duplication Level Percentage of deduplicated Percentage of total 1 73.13622049928112 28.08549910599572 2 12.75714582022709 9.797903284667731 3 5.0983464097305875 5.873544020528886 4 2.606805243313547 4.004222577036627 5 1.4864342575172367 2.8540747474797468 6 0.9195548500439499 2.1187441797660767 7 0.6608405865359407 1.776414791871874 8 0.45078785269067184 1.3848789830600603 9 0.34494521895893865 1.1921812086127141 >10 2.0580327637079394 15.473813656000997 >50 0.27837930563587115 7.518852318888418 >100 0.1925749594897446 13.786735473447816 >500 0.007449174650492465 1.8278202093595006 >1k 0.0016553721445538812 1.1494331933306992 >5k 2.758953574256469E-4 0.7470415196031671 >10k+ 5.517907148512937E-4 2.4088407303499655 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12712 1.3440303146925605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10024 1.0598300719381863 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7051 0.7454969909453465 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3064 0.32395444337775375 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2192 0.23175853129374546 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1973 0.20860382401576635 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1365 0.14432043577370554 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1196 0.1264521913445801 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1059 0.11196728313872101 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 987 0.10435477663637172 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0572925697707367E-4 0.0 12-13 0.0 0.0 0.0 1.0572925697707367E-4 0.0 14-15 0.0 0.0 0.0 1.585938854656105E-4 0.0 16-17 0.0 0.0 0.0 3.17187770931221E-4 0.0 18-19 0.0 0.0 0.0 5.286462848853684E-4 0.0 20-21 0.0 0.0 0.0 7.401047988395157E-4 0.0 22-23 0.0 0.0 0.0 0.0014802095976790314 0.0 24-25 0.0 0.0 0.0 0.003964847136640262 0.0 26-27 0.0 0.0 0.0 0.00750677724537223 0.0 28-29 0.0 0.0 0.0 0.017233868887263006 0.0 30-31 0.0 0.0 0.0 0.04356045387455435 0.0 32-33 0.0 0.0 0.0 0.08833679420434505 0.0 34-35 0.0 0.0 0.0 0.1565850295830461 0.0 36-37 0.0 0.0 0.0 0.26204996341767706 0.0 38-39 0.0 0.0 0.0 0.422652704765852 0.0 40-41 0.0 0.0 0.0 0.6001721272303586 0.0 42-43 0.0 0.0 0.0 0.7752069121559041 0.0 44-45 0.0 0.0 0.0 0.9830177667443425 0.0 46-47 0.0 0.0 0.0 1.233596105780007 0.0 48-49 0.0 0.0 0.0 1.4970734141668747 0.0 50-51 0.0 0.0 0.0 1.7829653250328819 0.0 52-53 0.0 0.0 0.0 2.0554824848912894 0.0 54-55 0.0 0.0 0.0 2.351101487399187 0.0 56-57 0.0 0.0 0.0 2.7116382536910084 0.0 58-59 0.0 0.0 0.0 3.1074885918131723 0.0 60-61 0.0 1.0572925697707367E-4 0.0 3.525700667785987 0.0 62-63 0.0 1.0572925697707367E-4 0.0 3.948089049409396 0.0 64-65 0.0 1.0572925697707367E-4 0.0 4.397967037846845 0.0 66-67 0.0 1.0572925697707367E-4 0.0 4.896956266150144 0.0 68-69 0.0 1.0572925697707367E-4 0.0 5.457004140357704 0.0 70-71 0.0 1.0572925697707367E-4 0.0 6.077793472698591 0.0 72-73 0.0 1.0572925697707367E-4 0.0 6.70434504954473 0.0 74-75 0.0 1.0572925697707367E-4 0.0 7.3386148621501945 0.0 76-77 0.0 1.0572925697707367E-4 0.0 8.007511006415651 0.0 78-79 0.0 1.0572925697707367E-4 0.0 8.76669993613953 0.0 80-81 0.0 1.0572925697707367E-4 0.0 9.580973808748462 0.0 82-83 0.0 1.0572925697707367E-4 0.0 10.409838318820231 0.0 84-85 0.0 1.0572925697707367E-4 0.0 11.217451248239609 0.0 86-87 0.0 1.0572925697707367E-4 0.0 12.072430884784715 0.0 88-89 0.0 1.0572925697707367E-4 0.0 12.99930641607423 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5720 0.0 48.403385 1 GTATCAA 9535 0.0 37.404377 1 TATCAAC 12240 0.0 29.059277 2 ATCAACG 12260 0.0 28.934507 3 TCAACGC 12305 0.0 28.82869 4 CAACGCA 12460 0.0 28.432005 5 AACGCAG 12690 0.0 27.97218 6 GATTACG 70 0.008484405 27.081211 6 GTACATG 7570 0.0 25.846664 1 GAGTACT 8785 0.0 24.977291 12-13 ACGCAGA 14245 0.0 24.71909 7 TACATGG 7600 0.0 24.648382 2 CGCAGAG 14285 0.0 24.58352 8 CATTCCG 255 2.0008883E-11 24.15941 9 CATGGGG 2280 0.0 24.128374 4 ACATGGG 7345 0.0 24.019064 3 GCAGAGT 14575 0.0 23.345276 9 GTACTTT 9685 0.0 22.755293 14-15 CTGCTCG 125 2.2913027E-4 22.747011 9 CTGCGGA 105 0.0022258272 22.56648 9 >>END_MODULE