FastQCFastQC Report
Fri 27 May 2016
SRR939348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945812
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123151.3020557996726623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103071.0897514516626983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72580.7673829471396006No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30410.32152267046728106No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22810.24116843516470504No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21470.22700071472977718No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.1489725230806968No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11450.12105999923874936No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11080.11714801673059763No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCG250.00503743656.952967
GGTATCA60400.046.8849681
GTATCAA99300.036.618261
CATTCCG1951.6370905E-1129.208199
TATCAAC125400.028.9533582
ATCAACG127850.028.2500343
TCAACGC129050.027.9873454
CAACGCA130700.027.597715
AACGCAG133350.027.0848676
CATGGGT8300.026.8753914
GTACATG76600.025.5989441
TACATGG76200.025.100662
CATGGGG22200.024.1579744
ACATGGG74250.023.906183
TTAGTAC1201.7201049E-423.7304023
TAGTACG1201.7201049E-423.7304027
ACGCAGA151950.023.55087
CGCAGAG151900.023.4648198
GAGTACT93700.023.40240112-13
ACTCCCG1452.3487177E-522.9121136
Produced by FastQC (version 0.11.5)