##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939348_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 945812 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.171010729405 33.0 31.0 34.0 30.0 34.0 2 32.35663218483166 34.0 31.0 34.0 30.0 34.0 3 32.40486375727946 34.0 31.0 34.0 30.0 34.0 4 36.007940267198975 37.0 35.0 37.0 35.0 37.0 5 35.965516402836926 37.0 35.0 37.0 35.0 37.0 6 36.02317373854424 37.0 35.0 37.0 35.0 37.0 7 35.96580927287875 37.0 35.0 37.0 35.0 37.0 8 35.96208760303316 37.0 35.0 37.0 35.0 37.0 9 37.619837769028095 39.0 37.0 39.0 35.0 39.0 10-11 37.55642876174123 39.0 37.0 39.0 35.0 39.0 12-13 37.484318765251444 39.0 37.0 39.0 34.5 39.0 14-15 38.66472671101657 40.0 38.0 41.0 34.0 41.0 16-17 38.63403456500869 40.0 38.0 41.0 34.0 41.0 18-19 38.845107695821156 40.0 38.0 41.0 34.5 41.0 20-21 38.89952813032611 40.0 38.0 41.0 35.0 41.0 22-23 38.81366381479617 40.0 38.0 41.0 34.5 41.0 24-25 38.73577571441259 40.0 38.0 41.0 34.0 41.0 26-27 38.533323218567745 40.0 38.0 41.0 34.0 41.0 28-29 38.352687954900134 40.0 38.0 41.0 34.0 41.0 30-31 38.02084399436674 40.0 37.0 41.0 33.5 41.0 32-33 37.645212262056305 40.0 36.0 41.0 33.0 41.0 34-35 37.42713985443196 40.0 35.0 41.0 33.0 41.0 36-37 37.231752716184616 40.0 35.0 41.0 33.0 41.0 38-39 37.0335663958588 40.0 35.0 41.0 32.0 41.0 40-41 36.761570481237285 40.0 35.0 41.0 31.0 41.0 42-43 36.41787797152077 40.0 35.0 41.0 30.5 41.0 44-45 35.92591973880644 40.0 35.0 41.0 29.5 41.0 46-47 35.25616401568176 39.0 35.0 41.0 25.0 41.0 48-49 34.63998236435994 39.0 35.0 41.0 20.5 41.0 50-51 34.29684281865741 39.0 35.0 41.0 14.0 41.0 52-53 34.07077569326674 39.0 35.0 41.0 9.0 41.0 54-55 33.799344372877485 39.0 34.0 40.0 7.5 41.0 56-57 33.56759482857058 38.0 34.0 40.0 2.0 41.0 58-59 33.29839280956469 38.0 34.0 40.0 2.0 41.0 60-61 32.99335385890642 38.0 33.0 40.0 2.0 41.0 62-63 32.627749489327684 38.0 33.0 40.0 2.0 41.0 64-65 32.23152116911183 37.0 32.5 40.0 2.0 41.0 66-67 31.866954003544045 36.5 32.0 39.5 2.0 41.0 68-69 31.484491632586604 36.0 31.0 39.0 2.0 40.0 70-71 31.118699593576736 36.0 31.0 39.0 2.0 40.0 72-73 30.62581358663244 35.0 31.0 38.0 2.0 40.0 74-75 30.117903452271698 35.0 30.0 37.5 2.0 39.5 76-77 28.15490922085996 33.0 27.0 35.5 2.0 38.5 78-79 29.052667443424276 34.0 29.0 36.0 2.0 39.0 80-81 29.02736907546109 34.0 29.0 36.0 2.0 38.5 82-83 28.67540959514153 34.0 29.0 36.0 2.0 37.0 84-85 28.255272189399165 34.0 29.0 35.0 2.0 37.0 86-87 27.715435519955342 34.0 27.5 35.0 2.0 36.5 88-89 27.193218631186745 34.0 26.0 35.0 2.0 36.0 90-91 26.769891902407664 34.0 25.5 35.0 2.0 36.0 92-93 26.406302203820633 33.0 25.0 35.0 2.0 35.0 94-95 26.012058950404523 33.0 24.0 35.0 2.0 35.0 96-97 25.498928433980538 33.0 21.0 35.0 2.0 35.0 98-99 24.83926351114175 33.0 9.5 35.0 2.0 35.0 100-101 21.83756338468956 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23599664919692032 1101 2 0.16050396405348266 1101 3 0.19195047286392253 1101 4 0.1667139152431929 1101 5 0.08967393305751159 1101 6 0.12052646876134787 1101 7 0.1930752323596252 1101 8 0.22621367395807823 1101 9 0.4082813447150002 1101 10-11 0.3633744979021145 1101 12-13 0.33546449988967453 1101 14-15 0.5196611798053041 1101 16-17 0.43490826352810075 1101 18-19 0.36433887103924434 1101 20-21 0.35445169301137724 1101 22-23 0.4108638944414622 1101 24-25 0.41954740096899457 1101 26-27 0.4256397652204029 1101 28-29 0.5041687271668138 1101 30-31 0.3075807138031408 1101 32-33 0.2586707644562196 1101 34-35 0.37509126103445567 1101 36-37 0.5475592248339964 1101 38-39 0.6717446187544382 1101 40-41 0.564889793753899 1101 42-43 0.2808921572461074 1101 44-45 0.2489189723004941 1101 46-47 0.29887673797422565 1101 48-49 0.5168380707738791 1101 50-51 0.4646261038483246 1101 52-53 0.27276773292216916 1101 54-55 0.3140012740039353 1101 56-57 0.5311409108569336 1101 58-59 0.39427973559705265 1101 60-61 0.32851162143369805 1101 62-63 0.231369910207178 1101 64-65 0.1707023038659088 1101 66-67 0.04380888388814341 1101 68-69 0.16370790081091968 1101 70-71 0.31833220387941097 1101 72-73 0.2226653933644691 1101 74-75 0.1136166866210715 1101 76-77 0.15948175489558736 1101 78-79 0.2721752828643318 1101 80-81 0.4643765819562553 1101 82-83 0.5422189366363668 1101 84-85 0.5552359141345278 1101 86-87 0.5750417445390497 1101 88-89 0.6927436678764778 1101 90-91 0.6301703573758921 1101 92-93 0.5798959138202946 1101 94-95 0.7451614338594474 1101 96-97 0.5901569406060254 1101 98-99 0.6200494069943581 1101 100-101 0.9701682955435231 1102 1 0.08160589480616665 1102 2 0.1468509170670984 1102 3 0.0832963642098079 1102 4 0.031915480444759226 1102 5 -0.03352199013841073 1102 6 0.11236072726227064 1102 7 0.021446027397090006 1102 8 0.08758413532854092 1102 9 0.16971550614915998 1102 10-11 0.2610494455770578 1102 12-13 0.24650281092798565 1102 14-15 0.2280286381727521 1102 16-17 0.11554496083146404 1102 18-19 0.12364666368036836 1102 20-21 0.3328042880306441 1102 22-23 0.19406072763829485 1102 24-25 0.2798440612656563 1102 26-27 0.28074032032095175 1102 28-29 0.22654413287555997 1102 30-31 0.3949635511859739 1102 32-33 0.44207841453053476 1102 34-35 0.3998721024819716 1102 36-37 0.3918262191009987 1102 38-39 0.2983621453719607 1102 40-41 0.5140055762030187 1102 42-43 0.3308314905187757 1102 44-45 0.6177969411784616 1102 46-47 0.5583073807382064 1102 48-49 0.39923865317445717 1102 50-51 0.541918547807434 1102 52-53 0.6426360861238507 1102 54-55 0.5074553341246641 1102 56-57 0.6202663999824125 1102 58-59 0.6132077545250638 1102 60-61 0.3900382996270437 1102 62-63 0.5592477880850524 1102 64-65 0.6974608806244831 1102 66-67 0.6116373183832415 1102 68-69 0.6688676059706218 1102 70-71 0.5708741397546824 1102 72-73 0.893918464617542 1102 74-75 0.8167728731105903 1102 76-77 0.8463246584051554 1102 78-79 0.7936513043403544 1102 80-81 0.691491575737917 1102 82-83 0.7154952432460107 1102 84-85 0.5640037729023852 1102 86-87 0.7019064880704597 1102 88-89 0.6618718870046969 1102 90-91 0.8743433182155194 1102 92-93 0.7701558707469189 1102 94-95 0.7708446928760395 1102 96-97 0.5803880808371673 1102 98-99 0.868893705838655 1102 100-101 0.6777379864465445 1103 1 0.08438634323879057 1103 2 0.12365479604489593 1103 3 0.10499395112478993 1103 4 0.12170586371340164 1103 5 0.20287844191419424 1103 6 0.102517880462905 1103 7 0.17112139446376062 1103 8 0.1407064275812715 1103 9 0.20795928352337256 1103 10-11 0.13442924830468428 1103 12-13 0.14359654497824437 1103 14-15 0.3173664938149585 1103 16-17 0.17636022273368468 1103 18-19 0.20412684742142773 1103 20-21 0.24147531083210083 1103 22-23 0.25337597173614057 1103 24-25 0.3099660805181088 1103 26-27 0.366138960067417 1103 28-29 0.3264043686053526 1103 30-31 0.5694567202443679 1103 32-33 0.26026765119803486 1103 34-35 0.34250940381492256 1103 36-37 0.45371864512384974 1103 38-39 0.26027118880274003 1103 40-41 0.6694925849508948 1103 42-43 0.6579184589100961 1103 44-45 0.6018175230251259 1103 46-47 0.3804521539699408 1103 48-49 0.1928445119977127 1103 50-51 0.43276888430994376 1103 52-53 0.43107422782286875 1103 54-55 0.3497769047070989 1103 56-57 0.33242915562343 1103 58-59 0.226324824163882 1103 60-61 0.1517268656634414 1103 62-63 0.06562916946644037 1103 64-65 0.19095190128942363 1103 66-67 0.23819965146731548 1103 68-69 0.2088769829365269 1103 70-71 0.26194465691215285 1103 72-73 0.31179582814707985 1103 74-75 0.4782436324074375 1103 76-77 0.4555633437307982 1103 78-79 0.333366909273348 1103 80-81 0.37128747835990694 1103 82-83 0.2737540949831221 1103 84-85 0.27608937629233665 1103 86-87 0.30807985511339453 1103 88-89 0.433283120372451 1103 90-91 0.4956963906555494 1103 92-93 0.3630145396925464 1103 94-95 0.6003278321417866 1103 96-97 0.5502616104933047 1103 98-99 0.22242460828260135 1103 100-101 0.1918805875456684 1104 1 0.15628235107699595 1104 2 0.0904910828643608 1104 3 0.12597418004120442 1104 4 0.13880804923650203 1104 5 0.1318475251724891 1104 6 0.14285434568085975 1104 7 0.17831396855576997 1104 8 0.05261721700875199 1104 9 0.13621760453871445 1104 10-11 0.2188605818758873 1104 12-13 0.18222972936299442 1104 14-15 0.18020566332750576 1104 16-17 0.14890172162081683 1104 18-19 0.09478768640403956 1104 20-21 0.2031614031122544 1104 22-23 0.2742740807121393 1104 24-25 0.16547462948707903 1104 26-27 0.2715452838691803 1104 28-29 0.365858044463792 1104 30-31 0.337203830471978 1104 32-33 0.3749177069829628 1104 34-35 0.3624532224487851 1104 36-37 0.342850354181401 1104 38-39 0.4225847906966038 1104 40-41 0.5446525772486126 1104 42-43 0.801254567331064 1104 44-45 0.8780123681582452 1104 46-47 0.7842493516977669 1104 48-49 0.9824843735831337 1104 50-51 1.0693711576164375 1104 52-53 1.094758383205118 1104 54-55 0.9790889095097057 1104 56-57 1.0084475844707512 1104 58-59 1.1041034514582861 1104 60-61 0.980638464963441 1104 62-63 0.9737150102896734 1104 64-65 1.0335697434039233 1104 66-67 0.9825928417420506 1104 68-69 1.0018087214439824 1104 70-71 1.0941155683302277 1104 72-73 1.0811648071979967 1104 74-75 0.8226943066404644 1104 76-77 0.8199082814612311 1104 78-79 1.0162267786743264 1104 80-81 0.9973422948359989 1104 82-83 1.262915678708648 1104 84-85 1.1406381980127769 1104 86-87 1.2166067366511335 1104 88-89 1.5508696719579298 1104 90-91 1.4411439726480602 1104 92-93 1.5419354836754202 1104 94-95 1.3497820177101971 1104 96-97 1.2368657244191503 1104 98-99 1.3875019709299217 1104 100-101 1.0568782372163383 1105 1 -0.08761446191419253 1105 2 -0.04960406063787559 1105 3 -0.019855082691634607 1105 4 0.061621322650594834 1105 5 0.10495171083528732 1105 6 0.08495548110702345 1105 7 0.018846837297900265 1105 8 0.013945155439564871 1105 9 -0.021811247877593587 1105 10-11 0.05295783123544595 1105 12-13 0.027478388778568785 1105 14-15 0.07695284647196132 1105 16-17 -0.018486798200292753 1105 18-19 -0.021039215380504572 1105 20-21 0.06552422387557755 1105 22-23 0.10681296851553412 1105 24-25 0.156005734302326 1105 26-27 0.10353328373891912 1105 28-29 -0.11454269258626937 1105 30-31 0.004918676766102692 1105 32-33 -0.04287244542032198 1105 34-35 0.0806082363431031 1105 36-37 0.10531385446363117 1105 38-39 0.10970758171739448 1105 40-41 -0.012270700073258922 1105 42-43 0.08880110786338946 1105 44-45 0.27449767600419506 1105 46-47 0.24810513303596338 1105 48-49 0.3504028855886929 1105 50-51 0.4679440050828916 1105 52-53 0.5317183502061198 1105 54-55 0.47935621227554037 1105 56-57 0.4199140590050803 1105 58-59 0.42471955041186504 1105 60-61 0.37407489303863883 1105 62-63 0.4238467620590285 1105 64-65 0.3892208223844307 1105 66-67 0.2768721554930771 1105 68-69 0.5372032711812871 1105 70-71 0.5317967100522516 1105 72-73 0.42741076373484077 1105 74-75 0.21747471131243046 1105 76-77 0.5732163227968208 1105 78-79 0.49497620879025916 1105 80-81 0.5352870757834189 1105 82-83 0.6141325168832807 1105 84-85 0.24111554376415612 1105 86-87 0.36713279392176545 1105 88-89 0.5411232975061075 1105 90-91 0.6486502956474993 1105 92-93 0.47314437061042014 1105 94-95 0.501218532624879 1105 96-97 0.27580508875417564 1105 98-99 0.47919292551287285 1105 100-101 0.2731003943089547 1106 1 0.21904412620413183 1106 2 0.24172858912598372 1106 3 0.094262180544753 1106 4 0.18657471564090145 1106 5 0.16919170586387366 1106 6 0.16461462850946162 1106 7 0.088120389373465 1106 8 0.13143365904383586 1106 9 0.21360075808809853 1106 10-11 0.2770831730201877 1106 12-13 0.27035140021282444 1106 14-15 0.3757387338694329 1106 16-17 0.37652314462844316 1106 18-19 0.2878370896157634 1106 20-21 0.28729202775328133 1106 22-23 0.21955144104847335 1106 24-25 0.1543473015407102 1106 26-27 0.330660893731455 1106 28-29 0.31656826316814346 1106 30-31 0.38668834492418824 1106 32-33 0.21635574249913958 1106 34-35 0.28068824504308054 1106 36-37 0.22474043288769252 1106 38-39 0.27901531226674336 1106 40-41 0.3430611418357685 1106 42-43 0.42536974445300046 1106 44-45 0.321737764246798 1106 46-47 0.20067473283710058 1106 48-49 -0.1070371467254958 1106 50-51 -0.037345890517407554 1106 52-53 0.10948991371594019 1106 54-55 -0.031744023866643545 1106 56-57 -0.10419341675927285 1106 58-59 -0.056105549016422174 1106 60-61 -0.09068632371502616 1106 62-63 -0.11846851882588538 1106 64-65 -0.10009063672568885 1106 66-67 0.14946801057635284 1106 68-69 0.3378689746364252 1106 70-71 0.14601883906045288 1106 72-73 0.3125982792705102 1106 74-75 0.36613553656721365 1106 76-77 0.6705472947083315 1106 78-79 0.6772212684472301 1106 80-81 0.7169534317366875 1106 82-83 0.5321352511611863 1106 84-85 0.39849964766671775 1106 86-87 0.5735047489304641 1106 88-89 0.8479760814981496 1106 90-91 0.8722202372333818 1106 92-93 0.8252020700884231 1106 94-95 0.7038075520133944 1106 96-97 0.6936369306631995 1106 98-99 0.8367657878046977 1106 100-101 0.8859359647787528 1107 1 0.06558213934579271 1107 2 0.02105798141501225 1107 3 0.14530306706158314 1107 4 0.039403614693455324 1107 5 0.09947691046612306 1107 6 0.08989233155443799 1107 7 0.16821132909196734 1107 8 0.1407797026086186 1107 9 0.19220253567844026 1107 10-11 0.20233360869220718 1107 12-13 0.10689622413830335 1107 14-15 0.03470873006411779 1107 16-17 0.04753683648531393 1107 18-19 0.00346383927219307 1107 20-21 0.11645830055789474 1107 22-23 0.16696116180633425 1107 24-25 0.10123954815409775 1107 26-27 0.022568058627562948 1107 28-29 0.09518567405230272 1107 30-31 -0.022552507175156222 1107 32-33 0.23028573442800138 1107 34-35 0.22175739267711947 1107 36-37 0.22276688102757447 1107 38-39 0.20114151294006177 1107 40-41 0.3675896586603429 1107 42-43 0.4840394359802431 1107 44-45 0.5688359098230578 1107 46-47 0.7873516520360013 1107 48-49 0.8808603812750633 1107 50-51 0.8583188395598356 1107 52-53 0.6960426148824936 1107 54-55 0.7897031586260113 1107 56-57 0.8450468536361058 1107 58-59 0.8050156611217147 1107 60-61 0.46064783854644276 1107 62-63 0.3918149164268314 1107 64-65 0.6809426634020426 1107 66-67 0.6033958453530381 1107 68-69 0.736730652706914 1107 70-71 0.49000402015216693 1107 72-73 0.851376119258294 1107 74-75 0.7718540500227533 1107 76-77 0.782635587673834 1107 78-79 1.1168143422921233 1107 80-81 1.0389950429596944 1107 82-83 0.9567100619256159 1107 84-85 0.9338884561673524 1107 86-87 0.9950777249036697 1107 88-89 1.1699516450844563 1107 90-91 1.1315293183592665 1107 92-93 0.9714157832744377 1107 94-95 1.041043755328623 1107 96-97 1.235048798878168 1107 98-99 1.5785261936401547 1107 100-101 1.2107358222612845 1108 1 -0.031558271891192646 1108 2 0.049667788834725 1108 3 0.1375845437656551 1108 4 0.07623885774457761 1108 5 0.06320077896123166 1108 6 -0.036919271054131286 1108 7 0.025097332975590803 1108 8 0.06221177055574856 1108 9 -0.10774688908625762 1108 10-11 0.03139337330856762 1108 12-13 -0.06641907533436608 1108 14-15 -0.09442399744904151 1108 16-17 -0.002793642517843864 1108 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6 120.0 7 238.0 8 500.0 9 947.0 10 1741.0 11 2836.0 12 4364.0 13 6539.0 14 7553.0 15 10530.0 16 14440.0 17 21274.0 18 18975.0 19 9694.0 20 7214.0 21 6668.0 22 6994.0 23 7385.0 24 8248.0 25 9159.0 26 10540.0 27 12149.0 28 14462.0 29 17350.0 30 20555.0 31 24755.0 32 29959.0 33 37451.0 34 49511.0 35 71059.0 36 101814.0 37 183272.0 38 204438.0 39 23022.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.36798284772692 22.975944827703213 16.59845061192982 19.057621712640046 2 14.61833852816416 23.884873526662805 41.439842167365185 20.05694577780785 3 16.960558757977275 30.53947296079982 33.04768812406694 19.45228015715597 4 10.826252997424435 20.447826840852095 40.640528984618506 28.085391177104963 5 10.022076268856813 41.32258842137761 35.450385488870936 13.204949820894637 6 24.575285574723097 41.79075757127209 19.116272578482825 14.517684275521987 7 23.095498894071973 37.925930311732145 23.187589076899005 15.790981717296884 8 24.485521435549558 36.10738709172647 21.269343167563957 18.137748305160013 9 26.396577755410167 17.132474529821994 23.25990788867132 33.21103982609652 10-11 22.079123546751365 29.955054492859045 28.549225427463387 19.416596532926206 12-13 24.01946687079462 29.003966961721776 29.385015203867155 17.59155096361645 14-15 21.484575105004534 29.2301429753355 29.811137220902044 19.474144698757918 16-17 17.18557207881117 32.46604744106872 30.784939813175022 19.56344066694509 18-19 16.871534723602576 29.399870164472432 36.30266902936313 17.425926082561862 20-21 18.386159194427645 28.212636337876873 36.59083411925415 16.81037034844134 22-23 18.173643387903727 28.57428326136695 36.15068322245858 17.101390128270737 24-25 17.442068235620013 29.19494486742066 36.69818811580749 16.664798781151838 26-27 17.098204279110682 29.048037436931967 37.01068085988217 16.84307742407518 28-29 16.691266340456664 28.907647608615665 37.19925312852872 17.201832922398953 30-31 17.249358223410148 28.882061128427218 36.79346424025071 17.07511640791193 32-33 16.915148042105617 29.39135895928578 36.60420887026174 17.08928412834686 34-35 17.259317451539946 29.30827392022872 36.59560002918229 16.83680859904904 36-37 17.603576108095417 28.961388903870418 36.002777046326756 17.43225794170741 38-39 16.97844077173145 29.923229712918992 35.83228433502318 17.26604518032638 40-41 17.136075639818348 29.486712431111435 36.33239728247392 17.044814646596297 42-43 17.121060728616516 29.613751830528724 35.57881714802303 17.68637029283173 44-45 17.3323345363656 29.92632198303138 34.99861757900285 17.742725901600174 46-47 17.85529259514576 30.173332543888215 34.023780624479286 17.947594236486744 48-49 17.77361674413097 30.500035947947374 33.43967934430944 18.286667963612217 50-51 17.79534410643976 30.71656946623642 32.81265198580691 18.67543444151692 52-53 17.79967900597582 31.358768973115165 32.533421018130454 18.308131002778566 54-55 17.944263764891964 31.04136974366999 32.211052513607356 18.80331397783069 56-57 17.74623286657391 31.45233936553987 32.08327870655056 18.718149061335655 58-59 17.68924804056633 30.932831047283333 32.497417554707134 18.880503357443203 60-61 17.934141898157204 31.40112350402996 32.088273202007635 18.576461395805204 62-63 17.516183346946114 31.173685838216752 32.56134340943273 18.748787405404403 64-65 17.471336798433516 31.00272570024487 32.30044660038147 19.225490900940144 66-67 17.338487987041823 31.19552300034256 31.865687895691746 19.60030111692387 68-69 17.3711536133448 30.86244385082995 32.01627394186866 19.750128593956585 70-71 17.533769924678477 30.8909698756201 32.10077689858027 19.474483301121154 72-73 17.510033706487125 30.466466908857154 31.99663358045785 20.026865804197875 74-75 17.085900792123592 31.11691329778011 31.430083356946202 20.367102553150097 76-77 16.948593931481184 30.663060710622027 32.10876366010191 20.279581697794885 78-79 16.824326610362313 30.474026550731015 31.84750246349169 20.854144375414986 80-81 16.842346773976118 30.540214347528895 31.552690022895952 21.064748855599042 82-83 16.94691968382723 30.476352594384508 31.619973102477022 20.956754619311237 84-85 16.633591030775673 30.19146511145978 31.482577933035316 21.692365924729227 86-87 16.445407590863866 30.193211405347046 31.650314345589635 21.711066658199446 88-89 16.750887971075617 29.711154083871154 31.66864288310083 21.869315061952395 90-91 16.547817556845704 29.40458373278341 31.823354553041224 22.224244157329657 92-93 16.664992620097863 29.558358320681066 31.545275382422723 22.23137367679835 94-95 16.37497726820975 28.841672552261972 31.798285494368862 22.98506468515942 96-97 16.477798970619954 28.60388745332053 31.524129531027306 23.394184045032205 98-99 16.140892693262508 28.767450613864064 31.365588510190186 23.72606818268324 100-101 16.058880747718888 28.27481206584832 32.08308222581702 23.583224960615766 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1970.0 1 1311.5 2 812.5 3 994.0 4 856.0 5 862.0 6 1567.5 7 3015.0 8 4433.5 9 4824.5 10 4054.0 11 3354.0 12 3428.5 13 3583.0 14 3728.5 15 3907.0 16 3966.0 17 4194.0 18 4537.0 19 4831.0 20 5311.5 21 5928.0 22 6646.0 23 7445.0 24 8386.0 25 9928.0 26 12632.0 27 15620.5 28 16763.5 29 18008.5 30 21319.0 31 24602.0 32 28415.5 33 33620.5 34 38636.0 35 41646.5 36 44522.0 37 47032.5 38 45566.0 39 42666.5 40 39575.0 41 36183.0 42 33146.0 43 31635.5 44 31000.0 45 29766.0 46 27453.5 47 24520.0 48 24146.5 49 22625.0 50 18819.0 51 15242.5 52 12146.0 53 10248.0 54 9080.0 55 8221.0 56 7210.5 57 6104.0 58 4913.0 59 3966.5 60 3335.5 61 2790.0 62 2322.5 63 1759.5 64 1292.0 65 995.5 66 747.0 67 574.5 68 443.5 69 311.0 70 221.0 71 192.0 72 172.5 73 162.0 74 146.0 75 120.0 76 84.0 77 53.5 78 42.5 79 30.0 80 23.5 81 20.0 82 10.0 83 4.5 84 4.5 85 2.5 86 2.0 87 3.0 88 2.5 89 1.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2886408715474111 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0020617205110529365 16-17 7.401047988395157E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.0572925697707367E-4 26-27 8.458340558165894E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0034890654802434314 36-37 0.021463039166345954 38-39 0.007242454102929546 40-41 0.018238296828545206 42-43 0.004969275077922462 44-45 7.929694273280525E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.17187770931221E-4 60-61 0.002220314396518547 62-63 0.0010044279412822 64-65 0.0 66-67 0.0 68-69 5.815109133739052E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2313356142658372 78-79 0.0 80-81 0.001215886455236347 82-83 0.0 84-85 0.0 86-87 0.10488342292125707 88-89 0.3546687925295936 90-91 0.3563604606412268 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.1145851395414734E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 945812.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.327355585616736 #Duplication Level Percentage of deduplicated Percentage of total 1 72.33670416967027 27.001378784328466 2 13.210311628303542 9.86211999092988 3 5.134265537214159 5.749456661357114 4 2.7429477130449484 4.09547938550332 5 1.5168843937757341 2.8310641574369755 6 0.9693467166706642 2.1709889747349576 7 0.6244015994782168 1.6315082372365872 8 0.4808611677926508 1.4359420638008955 9 0.3441951284058886 1.1563104553959254 >10 2.1281602361932976 15.606993293790461 >50 0.296750019839983 7.769099938252047 >100 0.2038019834226495 14.214677096884065 >500 0.008811522295642159 2.1237029331665083 >1k 0.0017054559281888051 1.1810020658011793 >5k 2.842426546981342E-4 0.7700757338590386 >10k+ 5.684853093962683E-4 2.4002002275226193 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12315 1.3020557996726623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10307 1.0897514516626983 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7258 0.7673829471396006 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3041 0.32152267046728106 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2281 0.24116843516470504 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2147 0.22700071472977718 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1409 0.1489725230806968 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1145 0.12105999923874936 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1108 0.11714801673059763 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0572925697707367E-4 0.0 10-11 0.0 0.0 0.0 2.1145851395414734E-4 0.0 12-13 0.0 0.0 0.0 2.1145851395414734E-4 0.0 14-15 0.0 0.0 0.0 2.643231424426842E-4 0.0 16-17 0.0 0.0 0.0 4.757816563968315E-4 0.0 18-19 0.0 0.0 0.0 8.458340558165894E-4 0.0 20-21 0.0 0.0 0.0 0.0010572925697707367 0.0 22-23 0.0 0.0 0.0 0.001903126625587326 0.0 24-25 0.0 0.0 0.0 0.004282034907571484 0.0 26-27 0.0 0.0 0.0 0.00782396501630345 0.0 28-29 0.0 0.0 0.0 0.01770965054365984 0.0 30-31 0.0 0.0 0.0 0.044459152558859474 0.0 32-33 0.0 0.0 0.0 0.08976413917353554 0.0 34-35 0.0 0.0 0.0 0.15965117803538126 0.0 36-37 0.0 0.0 0.0 0.26744215552350786 0.0 38-39 0.0 0.0 0.0 0.42920791869843056 0.0 40-41 0.0 0.0 0.0 0.6079432276181735 0.0 42-43 0.0 0.0 0.0 0.784352492884421 0.0 44-45 0.0 0.0 0.0 0.9938550155844924 0.0 46-47 0.0 0.0 0.0 1.2465479397596986 0.0 48-49 0.0 0.0 0.0 1.5125098856855272 0.0 50-51 0.0 0.0 0.0 1.8006749755765417 0.0 52-53 0.0 0.0 0.0 2.0743022926332086 0.0 54-55 0.0 0.0 0.0 2.372881714336464 0.0 56-57 0.0 0.0 0.0 2.7367489522230635 0.0 58-59 0.0 0.0 0.0 3.135242521769654 0.0 60-61 0.0 0.0 0.0 3.5566793400802696 0.0 62-63 0.0 0.0 0.0 3.9834026212397387 0.0 64-65 0.0 0.0 0.0 4.4371397275568505 0.0 66-67 0.0 0.0 0.0 4.93507166329038 0.0 68-69 0.0 0.0 0.0 5.494167974185144 0.0 70-71 0.0 0.0 0.0 6.118076319606857 0.0 72-73 0.0 0.0 0.0 6.74626669993614 0.0 74-75 0.0 0.0 0.0 7.383391202479986 0.0 76-77 0.0 0.0 0.0 8.05561781834022 0.0 78-79 0.0 0.0 0.0 8.81464815417863 0.0 80-81 0.0 0.0 0.0 9.631459528955013 0.0 82-83 0.0 0.0 0.0 10.463813104507027 0.0 84-85 0.0 0.0 0.0 11.27232473261071 0.0 86-87 0.0 0.0 0.0 12.129841871323265 0.0 88-89 0.0 0.0 0.0 13.059519227922674 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTCG 25 0.005037436 56.95296 7 GGTATCA 6040 0.0 46.884968 1 GTATCAA 9930 0.0 36.61826 1 CATTCCG 195 1.6370905E-11 29.20819 9 TATCAAC 12540 0.0 28.953358 2 ATCAACG 12785 0.0 28.250034 3 TCAACGC 12905 0.0 27.987345 4 CAACGCA 13070 0.0 27.59771 5 AACGCAG 13335 0.0 27.084867 6 CATGGGT 830 0.0 26.875391 4 GTACATG 7660 0.0 25.598944 1 TACATGG 7620 0.0 25.10066 2 CATGGGG 2220 0.0 24.157974 4 ACATGGG 7425 0.0 23.90618 3 TTAGTAC 120 1.7201049E-4 23.730402 3 TAGTACG 120 1.7201049E-4 23.730402 7 ACGCAGA 15195 0.0 23.5508 7 CGCAGAG 15190 0.0 23.464819 8 GAGTACT 9370 0.0 23.402401 12-13 ACTCCCG 145 2.3487177E-5 22.912113 6 >>END_MODULE