##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939347_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 481829 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.964759281819898 33.0 31.0 34.0 30.0 34.0 2 31.875785807828088 33.0 31.0 34.0 30.0 34.0 3 31.86972349111407 33.0 31.0 34.0 30.0 34.0 4 35.37327973202111 37.0 35.0 37.0 33.0 37.0 5 35.47210109810742 37.0 35.0 37.0 33.0 37.0 6 35.661531373163506 37.0 35.0 37.0 33.0 37.0 7 35.652650629165116 37.0 35.0 37.0 33.0 37.0 8 35.680220576179515 37.0 35.0 37.0 33.0 37.0 9 37.16809490503893 39.0 37.0 39.0 34.0 39.0 10-11 37.128496831863586 39.0 37.0 39.0 33.5 39.0 12-13 37.01661274020451 39.0 37.0 39.0 33.0 39.0 14-15 37.86260478302468 40.0 37.5 41.0 33.0 41.0 16-17 37.866143382818386 40.0 37.5 41.0 32.5 41.0 18-19 38.09535851930872 40.0 38.0 41.0 33.5 41.0 20-21 38.13337511855866 40.0 38.0 41.0 34.0 41.0 22-23 38.147547158846805 40.0 38.0 41.0 34.0 41.0 24-25 38.0231378767156 40.0 37.5 41.0 33.5 41.0 26-27 37.676836595555685 40.0 36.5 41.0 33.0 41.0 28-29 37.38182737029112 39.5 35.5 41.0 33.0 41.0 30-31 37.09953842545799 39.0 35.0 41.0 33.0 41.0 32-33 36.68830747007756 38.0 35.0 41.0 33.0 41.0 34-35 36.3958385651341 38.0 35.0 41.0 31.5 41.0 36-37 36.01714716216749 38.0 35.0 40.0 31.0 41.0 38-39 35.2079098601371 37.0 34.5 40.0 27.5 41.0 40-41 35.0711839677562 37.0 35.0 40.0 30.0 41.0 42-43 35.22836006134956 38.0 35.0 40.0 30.0 41.0 44-45 35.08556147512914 38.0 35.0 40.0 27.0 41.0 46-47 34.62350543450062 38.0 35.0 40.0 23.5 41.0 48-49 34.34429330737668 38.0 35.0 40.0 23.0 41.0 50-51 33.17367468541744 36.5 33.0 39.0 21.5 40.5 52-53 33.215568178752214 36.5 33.0 39.5 21.5 40.0 54-55 33.7420723949783 37.0 34.0 40.0 22.0 41.0 56-57 33.83776298230285 37.5 34.5 40.0 21.5 41.0 58-59 33.748249897785314 37.5 34.5 40.0 20.0 41.0 60-61 33.38509305168431 36.5 34.0 40.0 18.5 41.0 62-63 32.81166036083341 36.0 33.0 39.0 11.5 41.0 64-65 32.187154571435094 35.0 32.5 39.0 7.0 41.0 66-67 31.804361090760416 35.0 32.0 39.0 2.0 40.0 68-69 31.475037824622426 35.0 32.0 38.0 2.0 40.0 70-71 30.89261958080564 35.0 31.5 37.0 2.0 39.0 72-73 30.260844614998266 35.0 31.0 36.5 2.0 39.0 74-75 29.79551251585106 35.0 30.0 36.0 2.0 37.5 76-77 29.107590037129356 34.5 29.5 35.0 2.0 37.0 78-79 28.678064209501713 34.0 29.0 35.0 2.0 36.5 80-81 28.450820726855376 34.0 29.0 35.0 2.0 36.0 82-83 28.04233555888085 34.0 29.0 35.0 2.0 36.0 84-85 27.616094091472284 34.0 27.0 35.0 2.0 35.5 86-87 27.051395827150294 34.0 26.0 35.0 2.0 35.0 88-89 26.374015470218687 33.5 24.5 35.0 2.0 35.0 90-91 25.729620882097173 33.0 21.5 35.0 2.0 35.0 92-93 25.299106114409884 33.0 19.0 35.0 2.0 35.0 94-95 24.842259390779716 33.0 8.5 35.0 2.0 35.0 96-97 24.039467321394106 32.0 2.0 35.0 2.0 35.0 98-99 23.192436528311912 32.0 2.0 35.0 2.0 35.0 100-101 20.798724236191678 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19028447394186188 1101 2 0.45186775531991685 1101 3 0.4287194307298172 1101 4 0.19925339663129193 1101 5 0.4005022770184752 1101 6 0.5343446834148011 1101 7 0.5909399289649571 1101 8 0.5041236103446849 1101 9 0.5679736811783442 1101 10-11 0.7181138857564804 1101 12-13 0.6468338165870122 1101 14-15 0.8231655956524975 1101 16-17 0.6362467471376974 1101 18-19 0.7575333462430933 1101 20-21 0.7598203160173185 1101 22-23 0.8225910853151959 1101 24-25 0.9200788580468711 1101 26-27 0.7888162398947642 1101 28-29 0.881654525746562 1101 30-31 0.8415864461441558 1101 32-33 0.9550151351892282 1101 34-35 1.0176246851915423 1101 36-37 1.0523605679000028 1101 38-39 1.3028290861024274 1101 40-41 1.1081900522754466 1101 42-43 1.082704659365568 1101 44-45 1.0488125941669324 1101 46-47 0.7981970687460631 1101 48-49 0.7209099077221168 1101 50-51 1.0489083531171985 1101 52-53 0.7277821606877026 1101 54-55 0.3973769721427587 1101 56-57 0.569330373473008 1101 58-59 0.7269227949609061 1101 60-61 0.6994405513485233 1101 62-63 0.9888934320550575 1101 64-65 0.872038708068267 1101 66-67 0.8636879010886496 1101 68-69 0.9280789634763273 1101 70-71 1.0160624318624478 1101 72-73 0.858897599497368 1101 74-75 1.1050354013288484 1101 76-77 1.016593936651315 1101 78-79 1.043702964950736 1101 80-81 0.738894921347967 1101 82-83 0.5671089809055623 1101 84-85 0.8027638654849056 1101 86-87 0.6856524177243024 1101 88-89 0.7940685996584627 1101 90-91 0.8339469062772729 1101 92-93 0.5528429395560366 1101 94-95 0.7017339458187202 1101 96-97 0.9876786590334703 1101 98-99 0.7005582508450452 1101 100-101 0.3075351699323292 1102 1 0.056223533764555356 1102 2 0.14922623383759515 1102 3 0.19985319899537402 1102 4 0.31536007919108044 1102 5 0.21871408830187988 1102 6 0.24810890474067548 1102 7 0.16867430812226303 1102 8 0.2639601382658441 1102 9 0.40719953020080624 1102 10-11 0.2759463907319457 1102 12-13 0.3237822313548335 1102 14-15 0.36067583926421065 1102 16-17 0.5520415981929219 1102 18-19 0.3649050217254768 1102 20-21 0.5157110579208748 1102 22-23 0.511488606627232 1102 24-25 0.3705716156803547 1102 26-27 0.442026082276179 1102 28-29 0.2633873723531366 1102 30-31 0.26556288317654264 1102 32-33 0.2345193975959745 1102 34-35 0.3645212699447953 1102 36-37 0.4380392005241731 1102 38-39 1.110238543217612 1102 40-41 0.7746686976708759 1102 42-43 0.3559616489676358 1102 44-45 0.34439533594277805 1102 46-47 0.45142819655441 1102 48-49 0.4625660203645907 1102 50-51 0.5044643816822401 1102 52-53 0.5295696044143554 1102 54-55 0.6180429931514766 1102 56-57 0.6534796481720875 1102 58-59 0.5793668941084249 1102 60-61 0.7866762129528055 1102 62-63 0.8025581440100211 1102 64-65 0.7115838384947253 1102 66-67 0.6245887003564299 1102 68-69 0.7115928681814729 1102 70-71 0.8536998559000786 1102 72-73 0.5220956284668326 1102 74-75 0.5567600530460517 1102 76-77 0.5393161299017102 1102 78-79 0.6629465873046918 1102 80-81 0.45042949420266254 1102 82-83 0.27774690515072464 1102 84-85 0.20163946280719713 1102 86-87 0.16896996436599565 1102 88-89 0.134659376682972 1102 90-91 0.4086853615938395 1102 92-93 0.49207038498552436 1102 94-95 0.6047841474981404 1102 96-97 0.22025653708925574 1102 98-99 -0.0504182181154782 1102 100-101 -0.4519980406550417 1103 1 0.09667470578307658 1103 2 -0.019837306046724734 1103 3 0.12355390126173305 1103 4 -0.016368923674669134 1103 5 -0.024045430084065345 1103 6 0.059725167088068076 1103 7 -0.07325067849373568 1103 8 0.06588743427793986 1103 9 0.008393068090654765 1103 10-11 0.046350430075655424 1103 12-13 -0.1805610078881017 1103 14-15 0.07869332769098492 1103 16-17 0.1697998044219915 1103 18-19 0.10968788600646207 1103 20-21 0.01913578735412358 1103 22-23 -0.025041840660804837 1103 24-25 -0.025062051115774864 1103 26-27 0.015373027050117116 1103 28-29 0.09699013523064792 1103 30-31 -0.07746903897581348 1103 32-33 0.0423251563900493 1103 34-35 0.08782675056161082 1103 36-37 -0.18156389826549457 1103 38-39 0.38595944220252676 1103 40-41 0.3394210149399015 1103 42-43 0.24457527650402966 1103 44-45 0.18251979139767371 1103 46-47 -0.34641829158893955 1103 48-49 -0.4527290049862813 1103 50-51 -0.410618074255531 1103 52-53 -0.14612557629588707 1103 54-55 -0.23106421531350918 1103 56-57 -0.06250399710467036 1103 58-59 -0.4433176738770541 1103 60-61 -0.35372194792812905 1103 62-63 -0.6175236296061826 1103 64-65 -0.2870147779670731 1103 66-67 -0.48509186817490146 1103 68-69 -0.5348590596394018 1103 70-71 -0.693031298902941 1103 72-73 -0.4623605612395565 1103 74-75 -0.5799509470274309 1103 76-77 -0.6493090446151548 1103 78-79 -0.3207380541615663 1103 80-81 -0.0951915758875117 1103 82-83 0.08647379806822997 1103 84-85 0.04119679356520223 1103 86-87 -0.319908193995591 1103 88-89 -0.7061628732986556 1103 90-91 -0.7534814817766176 1103 92-93 -0.6960220538417232 1103 94-95 -0.636437523035017 1103 96-97 -0.542770556325813 1103 98-99 -0.25511993861556803 1103 100-101 0.3147686998423218 1104 1 0.07512181982098909 1104 2 0.28874209556736474 1104 3 0.021888547076677867 1104 4 -0.0322561172014062 1104 5 -0.022253760554598045 1104 6 0.020313722600754147 1104 7 -0.19670146571844072 1104 8 0.11772429193270284 1104 9 0.26995039358785533 1104 10-11 0.021597379216558465 1104 12-13 0.07701659148613516 1104 14-15 0.42627721640663907 1104 16-17 0.095852476335061 1104 18-19 0.06565721093287635 1104 20-21 0.39760879868023125 1104 22-23 0.3841617384501319 1104 24-25 0.19379645984300709 1104 26-27 0.2969690399247682 1104 28-29 0.28688860729064203 1104 30-31 0.3044366843248696 1104 32-33 0.4176376415255305 1104 34-35 0.33408353538685986 1104 36-37 0.3185576604341449 1104 38-39 0.7962596921906382 1104 40-41 0.3169987200173665 1104 42-43 0.44305949237114106 1104 44-45 0.46165291931442454 1104 46-47 0.625332523647117 1104 48-49 0.5811013096393509 1104 50-51 0.5432873953574315 1104 52-53 0.4053888865467883 1104 54-55 0.4912560760045537 1104 56-57 0.23048682257092423 1104 58-59 0.2688683215037244 1104 60-61 0.5341956458438446 1104 62-63 0.4186823648232618 1104 64-65 0.4411218328706852 1104 66-67 0.1004352473428618 1104 68-69 0.45803239901214354 1104 70-71 0.4114933273040613 1104 72-73 0.2054036571644282 1104 74-75 0.13845304009791803 1104 76-77 0.10308381196143657 1104 78-79 -0.3391356990938874 1104 80-81 0.06114083958955163 1104 82-83 0.12665951862063451 1104 84-85 -0.12118977366997186 1104 86-87 0.08119851868720573 1104 88-89 0.07334285845828603 1104 90-91 0.22055252294831362 1104 92-93 0.18219462718296597 1104 94-95 0.18004583663603668 1104 96-97 0.20373991622041743 1104 98-99 0.6735224621158977 1104 100-101 0.6852029815096792 1105 1 0.0034116582293002296 1105 2 -0.09366761225350828 1105 3 -0.02090655794765084 1105 4 0.009907609879064694 1105 5 -0.3318548118245417 1105 6 -0.06587706646767799 1105 7 -0.0993306672050025 1105 8 0.14264575467626628 1105 9 -0.0863264271418771 1105 10-11 0.09950069112752402 1105 12-13 0.18138387892222596 1105 14-15 0.255266190391815 1105 16-17 0.3566988307690835 1105 18-19 -0.09780551210267419 1105 20-21 0.15489787738174243 1105 22-23 0.08840737013606059 1105 24-25 0.5932277063781228 1105 26-27 0.4821090723663062 1105 28-29 0.30370995299829673 1105 30-31 0.3971388792615258 1105 32-33 0.48884732060074043 1105 34-35 0.5519371583669681 1105 36-37 0.4324623480303913 1105 38-39 0.6742856864825626 1105 40-41 0.41412569371507857 1105 42-43 0.2990257572829549 1105 44-45 0.19676677267047893 1105 46-47 0.5925419357983159 1105 48-49 0.6074061573900025 1105 50-51 0.5976141308768135 1105 52-53 0.7035327380549106 1105 54-55 0.6078017713415989 1105 56-57 0.3567085132265362 1105 58-59 0.12129014356806778 1105 60-61 0.23339025397886104 1105 62-63 0.3295554116581556 1105 64-65 0.5897138087243192 1105 66-67 0.5349002700314038 1105 68-69 0.46094546391574553 1105 70-71 0.2984920748144795 1105 72-73 0.32290411914829065 1105 74-75 0.5019855740280654 1105 76-77 0.5959461613033632 1105 78-79 0.7025147125850637 1105 80-81 0.6002092747988819 1105 82-83 0.4275521741038695 1105 84-85 0.2997288555725852 1105 86-87 0.5726800495990609 1105 88-89 0.6469702530746382 1105 90-91 0.8755250092427929 1105 92-93 0.32910292851963874 1105 94-95 0.4673356339791148 1105 96-97 0.7616547263963795 1105 98-99 0.47477957153421 1105 100-101 0.6070686776680283 1106 1 0.3115554452548288 1106 2 0.34718296918435954 1106 3 0.48094014723774947 1106 4 0.4862478424542118 1106 5 0.39699035748724043 1106 6 0.2625621933875095 1106 7 0.2574125493531767 1106 8 0.19053210002842036 1106 9 0.329182239573548 1106 10-11 0.38353674758916156 1106 12-13 0.5726111150810809 1106 14-15 0.2565369429698876 1106 16-17 0.5467906175020616 1106 18-19 0.6192371998113231 1106 20-21 0.6407824674497604 1106 22-23 0.6718839297791561 1106 24-25 0.7593488941204001 1106 26-27 0.712672957395192 1106 28-29 0.8563959034115172 1106 30-31 0.6128559859681744 1106 32-33 0.4013939453356272 1106 34-35 0.2556598234733727 1106 36-37 0.4120937383616976 1106 38-39 1.0139880195477318 1106 40-41 0.8650874431482691 1106 42-43 0.37251913839902073 1106 44-45 0.24391260752749844 1106 46-47 0.279845380543442 1106 48-49 0.04594378629367668 1106 50-51 -0.0063041752740033985 1106 52-53 0.4029129033441663 1106 54-55 0.2552730651322861 1106 56-57 0.4320577416328817 1106 58-59 0.3798105853145799 1106 60-61 0.5772796710782515 1106 62-63 0.33735362711928474 1106 64-65 0.02491456934745173 1106 66-67 0.10393230396689646 1106 68-69 0.35121277906237935 1106 70-71 0.1581424807239351 1106 72-73 0.42031324662579905 1106 74-75 0.441757387090302 1106 76-77 0.48029853459378913 1106 78-79 0.8036995293772833 1106 80-81 0.8289904493765157 1106 82-83 0.5128937231421169 1106 84-85 0.5976243430296151 1106 86-87 0.575632949474727 1106 88-89 0.9183170679652974 1106 90-91 0.9315935384609624 1106 92-93 1.061851776614425 1106 94-95 0.8734553589846001 1106 96-97 0.8789570740889161 1106 98-99 0.9017707219766855 1106 100-101 0.7310721882363005 1107 1 -0.0898766607227337 1107 2 0.016050342597189626 1107 3 0.05087880808550338 1107 4 0.021986060010611652 1107 5 0.1759460455338342 1107 6 0.014877445702765613 1107 7 0.011652167229158295 1107 8 0.02577548072634528 1107 9 0.11839548333224315 1107 10-11 0.08249381018259072 1107 12-13 -0.008697625318568214 1107 14-15 0.3978296183534695 1107 16-17 0.34277135666462044 1107 18-19 0.1406242893802343 1107 20-21 0.14280562856382772 1107 22-23 -0.11376408314158937 1107 24-25 -0.11699987995564953 1107 26-27 0.03656558810600785 1107 28-29 -0.08142518213684014 1107 30-31 -0.21085194001247487 1107 32-33 0.001029260880031302 1107 34-35 -0.15482925747024723 1107 36-37 -0.3562521663478364 1107 38-39 0.3297600853197977 1107 40-41 0.2102340042039259 1107 42-43 0.05773502816037279 1107 44-45 0.17683686999721715 1107 46-47 0.4075751446368372 1107 48-49 0.27949647439182 1107 50-51 0.3225948127158986 1107 52-53 0.4696450041671767 1107 54-55 0.509465432961008 1107 56-57 0.536061538818501 1107 58-59 0.5182231555453285 1107 60-61 0.5555596834491823 1107 62-63 0.5455738467031424 1107 64-65 0.6369122154360056 1107 66-67 0.4189591076300623 1107 68-69 0.3036749360699922 1107 70-71 0.5902055036960512 1107 72-73 0.445677931081395 1107 74-75 0.48305607611626655 1107 76-77 0.2917472210424741 1107 78-79 0.05531032644262268 1107 80-81 -0.14325014786407664 1107 82-83 -0.027466408127104103 1107 84-85 -0.22555953697244746 1107 86-87 0.010956225707094092 1107 88-89 -0.01739279956275297 1107 90-91 0.1685654117964681 1107 92-93 0.04657712497613886 1107 94-95 0.12240297156857594 1107 96-97 0.11462742339562482 1107 98-99 0.23519068039664504 1107 100-101 -0.0980694931781052 1108 1 0.07840095311939521 1108 2 0.0449635610265382 1108 3 0.15924259991703238 1108 4 0.3617310469330164 1108 5 0.4419283740161646 1108 6 0.31406512593883207 1108 7 0.19862822517295342 1108 8 0.33567672812759497 1108 9 0.3484437698321443 1108 10-11 0.3607678193033763 1108 12-13 0.3446635677603709 1108 14-15 0.16611938765130674 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2316 82-83 -0.9616277516148735 2316 84-85 -0.8339315598522425 2316 86-87 -0.9275893346001247 2316 88-89 -1.0916067105642284 2316 90-91 -1.204520308439509 2316 92-93 -1.5728775229759187 2316 94-95 -1.7112660900797607 2316 96-97 -1.9621233010234889 2316 98-99 -1.838082473187388 2316 100-101 -1.0302236481301925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2114.0 3 359.0 4 307.0 5 330.0 6 423.0 7 526.0 8 686.0 9 853.0 10 1078.0 11 1475.0 12 2229.0 13 3241.0 14 3380.0 15 3911.0 16 5082.0 17 6185.0 18 5649.0 19 4033.0 20 4035.0 21 4225.0 22 4699.0 23 5173.0 24 5729.0 25 6386.0 26 7358.0 27 8177.0 28 9580.0 29 11315.0 30 13226.0 31 15731.0 32 19154.0 33 24277.0 34 34616.0 35 68316.0 36 60942.0 37 78073.0 38 53392.0 39 5564.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.95449012079783 17.45752307228101 13.570559041108718 15.017427765812446 2 12.488592590744771 25.96544620380129 39.680738056780314 21.865223148673625 3 24.941793266312438 34.712417159345094 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31.588689084939947 30.418271893878853 22.721911078639938 24-25 17.2877966232068 32.24054792071407 33.16192620450149 17.30972925157764 26-27 20.82042008335152 28.051008636547873 35.17673224622995 15.951839033870648 28-29 16.051314988141932 28.929739122482108 39.268499522975134 15.750446366400823 30-31 15.043551635342602 36.64593175143873 35.22465826878353 13.085858344435138 32-33 13.82454667143344 35.92062489087689 32.886378941942354 17.368449495747317 34-35 15.394032899318841 31.47536819399376 33.60889974358894 19.52169916309846 36-37 19.08022065866173 31.648238493117702 34.84409963199238 14.427441216228187 38-39 21.894923289312597 27.572816545544526 36.44541689458467 14.086843270558202 40-41 15.290908882547761 31.070945991700967 39.64543152152737 13.9927136042239 42-43 13.874468302381462 35.61806135910352 35.475094356137895 15.032375982377127 44-45 14.764667754595036 36.57940313998637 31.596191000206332 17.05973810521226 46-47 14.136619424760315 33.62130054189246 29.83899541475615 22.40308461859108 48-49 16.90505918818696 32.50806164159411 31.87334415861892 18.713535011600012 50-51 20.26568759457578 33.64021393406062 31.93764147286014 14.156456998503462 52-53 14.603124312798135 33.653115610412435 36.0132859895759 15.730474087213539 54-55 12.054382270221154 32.938432826100325 37.01699888813746 17.990186015541067 56-57 16.01853404766642 36.72683217999027 30.14720359013837 17.10743018220494 58-59 14.17063255223876 32.94377556472857 32.96273265400291 19.92285922902976 60-61 15.255251941172428 30.19629318835359 34.30247932851779 20.245975541956184 62-63 21.477897097691326 29.40015341131742 32.376145373898396 16.745804117092856 64-65 18.968692257427787 30.308929600568607 36.03063554477723 14.691742597226376 66-67 14.7375179268252 31.539122169229227 36.950000833805824 16.773359070139744 68-69 13.944116794773171 35.849912128736094 34.71040008005354 15.4955709964372 70-71 14.558276779655893 34.64910314952998 31.049506312714126 19.743113758100005 72-73 14.672378034163982 32.35051954684259 29.752785333875288 23.224317085118134 74-75 16.53200674550317 32.37164053722221 32.31244020036396 18.783912516910657 76-77 19.11213648280835 28.72562137108035 34.59076832018273 17.571473825928567 78-79 16.099391203870123 28.908699335148363 37.55812632910416 17.433783131877348 80-81 14.794266209897227 35.0821643203054 34.14007735073724 15.983492119060122 82-83 14.519379457233484 33.7039792596668 31.70163680242082 20.075004480678896 84-85 15.984536270469382 29.810610994461612 31.763896776479296 22.44095595858972 86-87 18.63202779335052 29.686011833431632 33.490820649942584 18.191139723275263 88-89 21.02151109110273 25.805597760728972 35.36439217255559 17.808498975612704 90-91 16.940593533109382 27.986608869122747 37.497667912878434 17.575129684889433 92-93 15.190052856447474 32.24733190117481 34.8503373494227 17.71227789295501 94-95 15.515198133886162 32.88514915673626 32.22736070592135 19.37229200345623 96-97 15.877641722221528 29.603965762362783 30.084885325595263 24.433507189820432 98-99 18.148172081563228 28.66031859853333 31.344559185868597 21.846950134034845 100-101 21.307918603669652 28.52529332503822 32.27257995771159 17.89420811358054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7845.0 1 5021.0 2 3321.5 3 4294.0 4 3290.0 5 3082.0 6 6008.5 7 11677.0 8 15643.0 9 14338.5 10 9689.5 11 6223.5 12 5364.0 13 4859.0 14 4115.0 15 3272.5 16 2519.0 17 2065.5 18 1771.5 19 1493.5 20 1399.5 21 1390.0 22 1361.5 23 1483.5 24 1541.5 25 1641.0 26 1940.5 27 2215.0 28 2414.0 29 2687.0 30 3270.5 31 3888.5 32 4865.0 33 6380.5 34 7534.5 35 8141.5 36 9465.0 37 10665.5 38 10556.0 39 9917.5 40 8697.0 41 7408.0 42 6204.0 43 5733.5 44 6512.0 45 7199.0 46 10896.5 47 20367.0 48 69423.0 49 72048.5 50 28892.0 51 18014.5 52 6594.5 53 2744.0 54 1682.0 55 1042.5 56 629.5 57 409.0 58 329.0 59 266.5 60 231.5 61 238.0 62 224.0 63 167.0 64 121.0 65 104.0 66 89.0 67 90.5 68 83.0 69 76.5 70 74.5 71 69.0 72 86.0 73 82.0 74 60.5 75 48.5 76 37.0 77 29.5 78 26.0 79 20.0 80 15.5 81 13.0 82 9.0 83 4.0 84 2.5 85 2.0 86 1.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2644091576057066 2 0.1622982427375687 3 0.1629208702672525 4 0.18014689858850338 5 0.19259944918217875 6 0.09629972459108938 7 0.11062015777381602 8 0.12348779338728055 9 0.13033669621380198 10-11 0.11622380554096993 12-13 0.10667685008581883 14-15 0.12099728326854547 16-17 0.09661103835593125 18-19 0.12525190472138456 20-21 0.14403450186684488 22-23 0.1491192933592623 24-25 0.16821320426956451 26-27 0.15233620226262845 28-29 0.14984569214389337 30-31 0.14029873668874226 32-33 0.14963814963399877 34-35 0.3207569490420875 36-37 0.43241481936537646 38-39 0.4029437829603449 40-41 0.3929817424854046 42-43 0.4146699347693891 44-45 0.41882078496728087 46-47 0.4211037525761214 48-49 0.43334876065990213 50-51 0.4264998578333807 52-53 0.4307544792862198 54-55 0.4158114185738093 56-57 0.38343478703025347 58-59 0.373057661535524 60-61 0.3275020806136617 62-63 0.4302356230114834 64-65 0.42743379912790636 66-67 0.43604681328853184 68-69 0.4462163962733667 70-71 0.4405089772512655 72-73 0.4325185906203238 74-75 0.43625435579842636 76-77 0.4157076473188621 78-79 0.4041890380197124 80-81 0.4020098416658192 82-83 0.41321713720012704 84-85 0.4143586210045473 86-87 0.4155001048089675 88-89 0.42131129508601606 90-91 0.43760338211274125 92-93 0.442791944860106 94-95 0.4390561796820034 96-97 0.4406127485062128 98-99 0.436461898308321 100-101 0.4209999813211741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 481829.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.38023240610258 #Duplication Level Percentage of deduplicated Percentage of total 1 73.47240963423809 12.769675548794282 2 9.980535686564847 3.469280595397952 3 4.340661309004932 2.2632510704004947 4 2.5745435439380007 1.789846605330937 5 1.653869577158688 1.43723188102003 6 1.1499468612301924 1.1991806221709362 7 0.8657440024837896 1.05327823771504 8 0.6400534970087052 0.8899422824279983 9 0.5361642167106505 0.8386792824840348 >10 4.0648173578687175 14.072834968422407 >50 0.4083923432406291 4.8903241606462045 >100 0.2328552834266745 8.542242164751396 >500 0.02627085248916327 3.150702842709758 >1k 0.039406278733744915 15.909378638479627 >5k 0.008358907610188314 11.194842983714139 >10k+ 0.005970648292991653 16.529308115534764 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 22134 4.593745914006837 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 18645 3.869630096984615 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15167 3.147797247571234 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13627 2.8281817823335667 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10070 2.0899530746385127 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9272 1.9243341517426307 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9236 1.9168626213864255 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8572 1.7790543948164181 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7785 1.6157184395293764 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7767 1.6119826743512742 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6165 1.279499573500142 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5143 1.067391128387872 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4377 0.9084135658086168 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4118 0.8546600557459181 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3924 0.8143968088263679 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3895 0.8083780760394248 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3861 0.8013216307030088 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3650 0.7575301611152504 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3567 0.7403041327939995 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3557 0.7382287076950537 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3428 0.7114557239186516 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3396 0.7048143636020248 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3162 0.656249416286691 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3001 0.6228350721936621 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2629 0.5456292585128749 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2189 0.4543105541592557 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2094 0.4345940157192697 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2030 0.42131129508601606 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2016 0.4184056999474917 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1782 0.3698407526321579 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1751 0.36340693482542563 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1619 0.3360113235193399 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1593 0.3306152182620805 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1546 0.32086072029703483 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1500 0.3113137648418837 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1471 0.30529503205494063 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1339 0.2778994207488549 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1280 0.2656544126650741 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1238 0.25693762724950137 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1134 0.2353532062204641 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1130 0.23452303618088574 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1129 0.23431549367099117 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1110 0.23037218598299397 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1078 0.22373082566636712 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1062 0.2204101455080537 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1000 0.20754250989458914 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 952 0.19758046941964888 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 908 0.18844859898428695 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 894 0.1855430038457627 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 868 0.18014689858850338 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 867 0.1799393560786088 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 863 0.17910918603903045 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 724 0.15026077716368255 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 677 0.14050627919863684 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 653 0.1355252589611667 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 623 0.12929898366432904 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 621 0.12888389864453986 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 617 0.12805372860496153 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 599 0.12431796342685891 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 585 0.12141236828833465 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 579 0.12016711322896713 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 558 0.11580872052118076 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 557 0.11560117801128617 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 519 0.10771456263529178 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 513 0.10646930757592424 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 502 0.10418633996708375 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 502 0.10418633996708375 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 498 0.1033561699275054 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 498 0.1033561699275054 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 487 0.10107320231866493 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.1131376484188375E-4 0.0 12-13 0.0 0.0 0.0 4.150850197891783E-4 0.0 14-15 0.0 0.0 0.0 4.150850197891783E-4 0.0 16-17 0.0 0.0 0.0 4.150850197891783E-4 0.0 18-19 0.0 0.0 0.0 7.26398784631062E-4 0.0 20-21 0.0 0.0 0.0 0.001764111334104008 0.0 22-23 0.0 0.0 0.0 0.005084791492417434 0.0 24-25 0.0 0.0 0.0 0.011207295534307814 0.0 26-27 0.0 0.0 0.0 0.017018485811356312 0.0 28-29 0.0 0.0 0.0 0.036735024251342285 0.0 30-31 0.0 0.0 0.0 0.08094157885888978 0.0 32-33 0.0 0.0 0.0 0.14299678931737195 0.0 34-35 0.0 0.0 0.0 0.2760315381598036 0.0 36-37 0.0 0.0 0.0 0.44331080113484245 0.0 38-39 0.0 0.0 0.0 0.6286462624707105 0.0 40-41 0.0 0.0 0.0 0.8349435173059322 0.0 42-43 0.0 0.0 0.0 1.0205902924066421 0.0 44-45 0.0 0.0 0.0 1.1985579946412526 0.0 46-47 0.0 0.0 0.0 1.4585049882842254 0.0 48-49 0.0 0.0 0.0 1.77438468834379 0.0 50-51 0.0 0.0 0.0 2.0704440787084213 0.0 52-53 0.0 0.0 0.0 2.3202214893665594 0.0 54-55 0.0 0.0 0.0 2.5621122846487028 0.0 56-57 0.0 0.0 0.0 2.810436897737579 0.0 58-59 0.0 0.0 0.0 3.214522164502344 0.0 60-61 0.0 0.0 0.0 3.849187159759998 0.0 62-63 0.0 0.0 0.0 4.346251470957538 0.0 64-65 0.0 0.0 0.0 4.684234448320877 0.0 66-67 0.0 0.0 0.0 5.066112666526921 0.0 68-69 0.0 0.0 0.0 5.497801087107667 0.0 70-71 0.0 0.0 0.0 6.0641845966100005 0.0 72-73 0.0 0.0 0.0 6.72126418293627 0.0 74-75 0.0 0.0 0.0 7.331958018301099 0.0 76-77 0.0 0.0 0.0 7.82590919185022 0.0 78-79 0.0 0.0 0.0 8.35254831070774 0.0 80-81 0.0 0.0 0.0 8.938440816140165 0.0 82-83 0.0 0.0 0.0 9.551936475388572 0.0 84-85 0.0 0.0 0.0 10.617874806207181 0.0 86-87 0.0 0.0 0.0 11.813630976964857 0.0 88-89 0.0 0.0 0.0 12.576661014592315 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGACC 30 1.3164287E-4 63.17729 8 GGAACTT 40 5.4509286E-4 47.382973 8 ATGGGTC 85 7.3250703E-9 44.623554 5 CATATAG 60 8.3432795E-5 39.535095 1 TGGGAGG 240 0.0 39.48581 6 GGGATCT 85 3.705809E-7 39.02127 7 ACAACAT 25 0.0016080233 38.036777 90-91 GAGTAAT 50 0.0016345709 37.90638 6 GTACCCA 65 1.336589E-4 36.493935 1 GGGATAT 65 1.346436E-4 36.44844 7 ATATGAG 70 2.0815077E-4 33.844982 9 GATATGA 70 2.0815077E-4 33.844982 8 GCTTATG 130 8.365532E-9 32.84454 1 CTTATGT 130 8.416464E-9 32.824055 2 TACCTGG 145 6.7484507E-10 32.69829 2 CAGGGGC 30 0.0039319666 31.697313 64-65 GTCGGTG 30 0.0039319666 31.697313 94-95 AGTGCTG 30 0.0039339736 31.69401 74-75 ACCTGGG 150 9.713403E-10 31.608353 3 TATGTAC 135 1.2158125E-8 31.60835 4 >>END_MODULE