##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939346_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 487922 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.916019363750763 33.0 31.0 34.0 30.0 34.0 2 31.831196379749223 33.0 31.0 34.0 30.0 34.0 3 31.833711125958658 33.0 31.0 34.0 30.0 34.0 4 35.34770311648173 37.0 35.0 37.0 33.0 37.0 5 35.45538631174655 37.0 35.0 37.0 33.0 37.0 6 35.64875328433643 37.0 35.0 37.0 33.0 37.0 7 35.62490111124319 37.0 35.0 37.0 33.0 37.0 8 35.65302650833535 37.0 35.0 37.0 33.0 37.0 9 37.13449895680047 39.0 37.0 39.0 33.0 39.0 10-11 37.10018097154873 39.0 37.0 39.0 33.5 39.0 12-13 36.98864060239137 39.0 37.0 39.0 33.0 39.0 14-15 37.848778083382186 40.0 37.0 41.0 33.0 41.0 16-17 37.863692352466174 40.0 37.5 41.0 32.5 41.0 18-19 38.09170113255807 40.0 38.0 41.0 33.5 41.0 20-21 38.06595111513725 40.0 37.5 41.0 33.5 41.0 22-23 38.00661068777387 40.0 37.0 41.0 33.0 41.0 24-25 37.97759887850927 40.0 37.0 41.0 33.0 41.0 26-27 37.634217149462415 40.0 36.5 41.0 33.0 41.0 28-29 37.351753968872075 39.5 35.5 41.0 33.0 41.0 30-31 37.06917396633068 39.0 35.0 41.0 33.0 41.0 32-33 36.67449612847955 38.0 35.0 41.0 33.0 41.0 34-35 36.37597300388177 38.0 35.0 41.0 31.5 41.0 36-37 35.99642770770738 38.0 35.0 40.0 31.0 41.0 38-39 35.45973741704617 37.5 35.0 40.0 30.5 41.0 40-41 35.212527822069916 37.0 35.0 40.0 30.0 41.0 42-43 35.17446333635294 37.5 35.0 40.0 30.0 41.0 44-45 34.98477318095925 38.0 35.0 40.0 26.0 41.0 46-47 34.56106611302626 38.0 35.0 40.0 23.5 41.0 48-49 34.345922094105205 38.0 35.0 40.0 23.0 41.0 50-51 33.15622169117195 36.5 33.0 39.0 21.5 40.0 52-53 33.21371243764372 36.5 33.0 39.5 21.5 40.0 54-55 33.7594912711458 37.0 34.0 40.0 22.0 41.0 56-57 33.84856903357504 37.5 34.5 40.0 21.5 41.0 58-59 33.75875549780498 37.5 34.5 40.0 20.5 41.0 60-61 33.41154323846844 36.5 34.0 40.0 19.0 41.0 62-63 32.869570341161086 36.0 33.0 39.0 13.5 41.0 64-65 32.27266960702735 35.0 32.5 39.0 7.5 41.0 66-67 31.887986194514696 35.0 32.0 39.0 2.0 40.0 68-69 31.54564049171794 35.0 32.0 38.0 2.0 40.0 70-71 30.9814140374896 35.0 31.5 37.0 2.0 39.0 72-73 30.36012723345125 35.0 31.0 36.5 2.0 39.0 74-75 29.910933509864282 35.0 31.0 36.0 2.0 37.5 76-77 29.25895225056464 34.5 29.5 35.0 2.0 37.0 78-79 28.792955636351714 34.0 29.0 35.0 2.0 36.5 80-81 28.44718828009395 34.0 29.0 35.0 2.0 36.0 82-83 28.03572189817225 34.0 28.5 35.0 2.0 36.0 84-85 27.613879882440227 34.0 27.0 35.0 2.0 35.5 86-87 27.197234394021997 34.0 26.0 35.0 2.0 35.0 88-89 26.571013399682734 33.5 24.5 35.0 2.0 35.0 90-91 25.96881161333164 33.0 23.5 35.0 2.0 35.0 92-93 25.581835826218125 33.0 20.0 35.0 2.0 35.0 94-95 25.12770278856047 33.0 14.0 35.0 2.0 35.0 96-97 24.37912309754428 33.0 2.0 35.0 2.0 35.0 98-99 23.59808022593775 32.0 2.0 35.0 2.0 35.0 100-101 21.45680457122245 29.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26586487555397653 1101 2 0.08975426208146331 1101 3 0.08587334256109003 1101 4 0.1396237429511089 1101 5 0.2430649389744417 1101 6 0.413244997326359 1101 7 0.20003500967907684 1101 8 0.1284041339240929 1101 9 0.14501709803876395 1101 10-11 0.30979891531313086 1101 12-13 0.43160835818188303 1101 14-15 0.5121377789411881 1101 16-17 0.516451022668015 1101 18-19 0.5712453952933032 1101 20-21 0.6185550105823623 1101 22-23 0.5261154478874488 1101 24-25 0.619842904389607 1101 26-27 0.48565399382004415 1101 28-29 0.4038282634956971 1101 30-31 0.45487726710107523 1101 32-33 0.32143227969942956 1101 34-35 0.5914138021060538 1101 36-37 0.48011493368546354 1101 38-39 0.8019332070012908 1101 40-41 0.4601248793332715 1101 42-43 0.43702496570517724 1101 44-45 0.6455333403921131 1101 46-47 0.8652148483796438 1101 48-49 0.5290870447572829 1101 50-51 0.04637322316746406 1101 52-53 0.13014434739179848 1101 54-55 0.045695979960193256 1101 56-57 0.13628429777021722 1101 58-59 0.23284700198546915 1101 60-61 0.27428472589262043 1101 62-63 0.33886855936820126 1101 64-65 0.16469953426525308 1101 66-67 0.24233692535369755 1101 68-69 0.4410816312674548 1101 70-71 0.6870736579073196 1101 72-73 0.5884654338914892 1101 74-75 0.5238806889690863 1101 76-77 0.21359762615221456 1101 78-79 0.022160628269705995 1101 80-81 0.049575787094557455 1101 82-83 -0.10410603445428634 1101 84-85 0.031181576891505358 1101 86-87 0.054251735318764815 1101 88-89 0.07719067352005737 1101 90-91 -0.146167616716685 1101 92-93 0.011730747672057618 1101 94-95 0.014681220811734619 1101 96-97 -0.30224534267017233 1101 98-99 -0.41379303018553415 1101 100-101 -0.33935088420839676 1102 1 0.5231508919187853 1102 2 0.4397895046422171 1102 3 0.37677769731577015 1102 4 0.4278159528128924 1102 5 0.48124335826897635 1102 6 0.5536176490992091 1102 7 0.37709057034225424 1102 8 0.5597424318616362 1102 9 0.5270617794766608 1102 10-11 0.5337347432068498 1102 12-13 0.6019831551540946 1102 14-15 0.7134647379678754 1102 16-17 0.6249521412362427 1102 18-19 0.46510399601041286 1102 20-21 0.497665365766089 1102 22-23 0.571440445589019 1102 24-25 0.609469333245606 1102 26-27 0.4093232040802164 1102 28-29 0.5006457070309835 1102 30-31 0.6271674206360558 1102 32-33 0.8113191970479718 1102 34-35 0.661937230747526 1102 36-37 0.7944785756210493 1102 38-39 0.7158570830700555 1102 40-41 0.4904717887139256 1102 42-43 0.5016797418383021 1102 44-45 0.6089806757480645 1102 46-47 0.8087501369089622 1102 48-49 1.164234603826877 1102 50-51 0.7833947670980805 1102 52-53 1.0924577916625893 1102 54-55 1.0370921982801988 1102 56-57 0.7466318200649695 1102 58-59 0.6241083792034559 1102 60-61 0.5536126655818734 1102 62-63 0.7869853807956844 1102 64-65 0.8786448623275334 1102 66-67 0.8566070978890181 1102 68-69 0.9982358711007357 1102 70-71 1.214478579607544 1102 72-73 0.9102453364381518 1102 74-75 0.822622682778654 1102 76-77 0.9082821286191951 1102 78-79 1.0831685655073038 1102 80-81 0.7853102114149912 1102 82-83 0.701914314293834 1102 84-85 0.6374838661256703 1102 86-87 0.7622364737576746 1102 88-89 1.019362228176675 1102 90-91 1.077875471144548 1102 92-93 1.1868632903462029 1102 94-95 1.2892054462415388 1102 96-97 1.2432098992776695 1102 98-99 0.7507667442620765 1102 100-101 0.2938736568721687 1103 1 -0.07590074137942437 1103 2 0.33131806740365377 1103 3 0.11112326042845666 1103 4 0.33416074956865316 1103 5 0.20027774853624436 1103 6 0.11246747507232868 1103 7 0.06973354380714625 1103 8 0.12339795442127155 1103 9 0.071989454541324 1103 10-11 0.17149538439215206 1103 12-13 0.15829442379412484 1103 14-15 0.380115438865154 1103 16-17 0.1459311622152626 1103 18-19 0.19600095293476727 1103 20-21 0.24699480345168467 1103 22-23 0.4240284968072032 1103 24-25 0.17674908545686918 1103 26-27 0.28783168190376784 1103 28-29 0.4768167962157719 1103 30-31 0.47218769716318576 1103 32-33 0.35556034402883796 1103 34-35 0.5267949757422556 1103 36-37 0.491496169898916 1103 38-39 0.8825796299295874 1103 40-41 0.9008798737795445 1103 42-43 0.43618017291407796 1103 44-45 0.6524794144522801 1103 46-47 0.5436225704115287 1103 48-49 0.7106470954448483 1103 50-51 0.026864767417400515 1103 52-53 0.08232751098436353 1103 54-55 0.17731156795162661 1103 56-57 0.2870172337380481 1103 58-59 0.2648865508035456 1103 60-61 0.41225972628372887 1103 62-63 0.2620628147772237 1103 64-65 0.4162522321266806 1103 66-67 -0.023162675716370984 1103 68-69 0.09042222356106322 1103 70-71 0.1323107474397105 1103 72-73 0.18841693652755964 1103 74-75 0.05231422411402775 1103 76-77 0.32325642373047714 1103 78-79 0.42995305955207286 1103 80-81 0.4645020241684499 1103 82-83 0.61284926974934 1103 84-85 0.6899713329966985 1103 86-87 0.8554047210355122 1103 88-89 0.8413917967815863 1103 90-91 0.9500136069813188 1103 92-93 0.911302093552127 1103 94-95 0.9202365156287726 1103 96-97 0.2264697900718069 1103 98-99 0.4315551339545749 1103 100-101 0.1821052882544869 1104 1 0.1890565235778432 1104 2 0.4035946796802712 1104 3 0.5040836333888272 1104 4 0.34982508523298605 1104 5 0.258381866640363 1104 6 0.27288705549190695 1104 7 0.25444216851576584 1104 8 0.5521625231858422 1104 9 0.31884260139446496 1104 10-11 0.44603306892983596 1104 12-13 0.43003490553460466 1104 14-15 0.478670217419932 1104 16-17 0.3027299190140198 1104 18-19 0.17012682706064197 1104 20-21 0.23185105830794583 1104 22-23 0.11141000418871272 1104 24-25 0.3135789222867018 1104 26-27 0.6004190944911798 1104 28-29 0.3720460114449864 1104 30-31 0.5345342395097248 1104 32-33 0.6036023020707972 1104 34-35 0.8248291637764495 1104 36-37 0.5144176928204374 1104 38-39 0.7976379049878659 1104 40-41 0.3265752894749596 1104 42-43 0.3738258605597693 1104 44-45 0.5144296864025577 1104 46-47 0.659830806619766 1104 48-49 0.6585725033702587 1104 50-51 0.6424358629884992 1104 52-53 0.5908900595469078 1104 54-55 0.7099689106089642 1104 56-57 0.7727048794256959 1104 58-59 0.76479331071031 1104 60-61 0.8569916610156625 1104 62-63 0.8071832498976619 1104 64-65 0.9349157907902423 1104 66-67 0.8122996397459445 1104 68-69 0.7273530704919118 1104 70-71 0.44670080182976335 1104 72-73 0.23628795439857697 1104 74-75 0.2184369902367962 1104 76-77 0.3556185060925614 1104 78-79 0.4686632382622484 1104 80-81 0.4538338035002276 1104 82-83 0.7595703264703992 1104 84-85 0.886692389717755 1104 86-87 0.6978833535141433 1104 88-89 0.5826194580092476 1104 90-91 0.4670687740364805 1104 92-93 0.4441692264192625 1104 94-95 1.0833911387833943 1104 96-97 0.6100439936460091 1104 98-99 0.5776312800307224 1104 100-101 0.7003881165373151 1105 1 0.16630748143224494 1105 2 0.2498686260404206 1105 3 0.15721581729687628 1105 4 0.31853198178470876 1105 5 0.3466385716212059 1105 6 0.1289502738827082 1105 7 0.261970904147951 1105 8 0.2359843622663007 1105 9 0.38550926806113495 1105 10-11 0.33184724517504094 1105 12-13 0.5106048289062599 1105 14-15 0.33216159673028045 1105 16-17 0.300598692960385 1105 18-19 0.3237187810836275 1105 20-21 0.28250239930410714 1105 22-23 0.4039914218132381 1105 24-25 0.3799582326315303 1105 26-27 0.5610512783992263 1105 28-29 0.41450769726874626 1105 30-31 0.3032076111572337 1105 32-33 0.41597299172597246 1105 34-35 0.764278397492923 1105 36-37 0.834939723471777 1105 38-39 0.9338591693556779 1105 40-41 0.8224421477125077 1105 42-43 0.6698028720540208 1105 44-45 0.8345111040270794 1105 46-47 0.5622781246274329 1105 48-49 0.6735293999219749 1105 50-51 0.5692749434482636 1105 52-53 0.29049254996836993 1105 54-55 0.2900838531376948 1105 56-57 0.29872499436822864 1105 58-59 0.32292549461835307 1105 60-61 0.27551177595819354 1105 62-63 0.2714169663727546 1105 64-65 0.2650259440469398 1105 66-67 0.5377498313168232 1105 68-69 0.4755475149363555 1105 70-71 0.26296995891788555 1105 72-73 0.49280615363229074 1105 74-75 0.5816074883210867 1105 76-77 0.36766352524964674 1105 78-79 0.47133086278332215 1105 80-81 0.5614822709331797 1105 82-83 0.5507963801102456 1105 84-85 0.5625448801392103 1105 86-87 0.5404456140505403 1105 88-89 0.5165083468981351 1105 90-91 0.6729835249943363 1105 92-93 0.7201749735456993 1105 94-95 0.4623327096646186 1105 96-97 0.13274993234332655 1105 98-99 0.35373281259776945 1105 100-101 0.2533239402921055 1106 1 0.10741909667725835 1106 2 0.14055374400775378 1106 3 0.08548714216075837 1106 4 0.210877716811936 1106 5 0.15113040465205785 1106 6 0.18060635373752376 1106 7 0.17163515097190896 1106 8 0.06830287406303626 1106 9 0.30024611016639824 1106 10-11 0.2652728350117002 1106 12-13 0.1720816891603434 1106 14-15 0.2618281121567705 1106 16-17 0.24600477281519062 1106 18-19 0.14240752881502772 1106 20-21 0.21261129222606456 1106 22-23 0.15749187553374355 1106 24-25 0.21147365912880645 1106 26-27 0.3481383927367929 1106 28-29 0.3607009822052163 1106 30-31 0.2950020757670302 1106 32-33 0.3378128283865891 1106 34-35 0.3100338421390205 1106 36-37 0.5062305583175117 1106 38-39 0.7993922909527811 1106 40-41 0.8652302602351938 1106 42-43 0.5431241061737992 1106 44-45 0.6115641893265362 1106 46-47 0.5625208650993017 1106 48-49 0.5901514507386736 1106 50-51 0.6038393717604222 1106 52-53 0.6083169601317095 1106 54-55 0.46558294569668135 1106 56-57 0.5077925987239453 1106 58-59 0.3946178721138125 1106 60-61 0.37576359084022926 1106 62-63 0.38712477036549586 1106 64-65 0.6676643288019335 1106 66-67 0.2784984701552986 1106 68-69 0.17337646230477688 1106 70-71 0.30447857960754376 1106 72-73 0.3078084222348316 1106 74-75 0.24908026509059766 1106 76-77 0.46234365229138774 1106 78-79 0.42421879381780414 1106 80-81 0.39529579180431895 1106 82-83 0.0841902095113305 1106 84-85 -0.05588070969745118 1106 86-87 -0.19012834239228837 1106 88-89 -0.44007060047028546 1106 90-91 -0.17193707391673385 1106 92-93 -0.5288132297210879 1106 94-95 -0.38514687291251093 1106 96-97 -0.07042384261130152 1106 98-99 0.27669560751610334 1106 100-101 0.1874641399291299 1107 1 0.15803199255330824 1107 2 0.2307519668375555 1107 3 0.2568109061161046 1107 4 -0.02065110524320346 1107 5 0.17047421873271418 1107 6 0.19938056198541432 1107 7 0.3069962810698783 1107 8 0.11424044526376065 1107 9 0.41364779619966185 1107 10-11 0.5154847283814945 1107 12-13 0.39797141347111875 1107 14-15 0.42706848581820367 1107 16-17 0.32408634037044237 1107 18-19 0.38289738983120714 1107 20-21 0.3510574075142827 1107 22-23 0.1958400186187319 1107 24-25 0.2181243768321579 1107 26-27 0.5792069732790566 1107 28-29 0.602940672339642 1107 30-31 0.4986612236367094 1107 32-33 0.4337321722006706 1107 34-35 0.5647570137042948 1107 36-37 0.3506370290397669 1107 38-39 0.3784171257670934 1107 40-41 0.1387253616250277 1107 42-43 0.17252715926106532 1107 44-45 0.05466056663343721 1107 46-47 0.00998953677849812 1107 48-49 -0.13792100312325317 1107 50-51 -0.3570013664487348 1107 52-53 -0.4084317297748825 1107 54-55 -0.39561550497545284 1107 56-57 -0.27075832403750866 1107 58-59 -0.31070452478753197 1107 60-61 -0.2623156982916939 1107 62-63 -0.06760751489310479 1107 64-65 -0.21087064499619146 1107 66-67 -0.516502669056365 1107 68-69 -0.3552154720766332 1107 70-71 -0.7909326758037096 1107 72-73 -0.6396716415610193 1107 74-75 -0.4344490126492069 1107 76-77 -0.6715820711080163 1107 78-79 -0.500283370684361 1107 80-81 -0.3842325745661519 1107 82-83 -0.7052492983492265 1107 84-85 -1.0263956333702708 1107 86-87 -0.8771960115652249 1107 88-89 -1.1117384063486213 1107 90-91 -1.0243453673776592 1107 92-93 -0.6636229813729457 1107 94-95 -1.1084847630925054 1107 96-97 -0.8690180554160385 1107 98-99 -0.7596703072708664 1107 100-101 -0.5852107290615294 1108 1 0.18406786144631937 1108 2 0.23928628965759557 1108 3 0.34870948372896393 1108 4 0.21676386074319254 1108 5 0.2732571500870762 1108 6 0.24322719154632466 1108 7 0.1060067943661096 1108 8 0.08127816944434585 1108 9 0.1855092680611321 1108 10-11 0.19322127754661267 1108 12-13 0.19218910054594573 1108 14-15 0.4382620541546274 1108 16-17 0.41780928409338713 1108 18-19 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-1.2810944711263623 2316 70-71 -1.0503297827617928 2316 72-73 -1.389143714203822 2316 74-75 -1.499410473952132 2316 76-77 -0.9080671307638113 2316 78-79 -0.8749798124539687 2316 80-81 -0.9714197776070144 2316 82-83 -0.8444637424417216 2316 84-85 -0.9697226315753156 2316 86-87 -0.8101228408335146 2316 88-89 -1.045773883067021 2316 90-91 -0.9618123719178335 2316 92-93 -0.9447970918153494 2316 94-95 -0.8007823029510241 2316 96-97 -0.8926428046505457 2316 98-99 -1.1870802956564646 2316 100-101 -1.1662475783910615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1856.0 3 364.0 4 329.0 5 345.0 6 403.0 7 492.0 8 662.0 9 826.0 10 1056.0 11 1485.0 12 2196.0 13 3436.0 14 3447.0 15 3865.0 16 5071.0 17 6629.0 18 5597.0 19 3988.0 20 3960.0 21 4314.0 22 4673.0 23 5223.0 24 5910.0 25 6539.0 26 7433.0 27 8457.0 28 9647.0 29 11174.0 30 13118.0 31 15813.0 32 19166.0 33 24082.0 34 34168.0 35 68161.0 36 61700.0 37 79287.0 38 56405.0 39 6645.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.89380894651406 17.582756282491214 13.594838391517866 14.928596379476852 2 12.51713073520602 25.869461746145074 39.786799281124594 21.82660823752431 3 24.888023488906992 34.811512190718105 29.13769366345288 11.162770656922026 4 10.677771006838649 17.157354573564202 39.17747452177678 32.987399897820374 5 9.852689811404382 45.33045785377325 31.339170474761925 13.47768186006045 6 21.779311560993513 37.669961839965715 20.158996725331825 20.391729873708947 7 22.08603676392181 42.63286113868086 19.210625610066195 16.070476487331128 8 22.574698029243486 30.118942640936773 21.87314152123536 25.43321780858438 9 28.135394408192894 22.833964260666637 21.804930666349495 27.225710664790974 10-11 21.615007069423896 28.056641516127414 25.436904750919435 24.89144666352926 12-13 32.82756768007776 27.222653434322407 24.40122753609963 15.548551349500197 14-15 28.457064231659267 26.972539522561107 31.031272524450728 13.539123721328906 16-17 15.731100880745611 32.09318062974849 34.028360213675654 18.14735827583025 18-19 14.739037439734885 34.52568201346494 36.621503290357374 14.113777256442805 20-21 16.687123106753905 32.38239085028039 32.616588891178914 18.313897151786783 22-23 15.299627446134537 31.526691489918075 30.502552911775595 22.671128152171796 24-25 17.26890290927588 32.22447614984494 33.24961439377779 17.25700654710139 26-27 20.798049795041827 27.914718529921227 35.404486004695016 15.882745670341933 28-29 16.128231712131885 28.942276040080355 39.22448138269235 15.705010865095407 30-31 14.989314661678806 36.61205043643762 35.33524479884492 13.063390103038655 32-33 13.829897409342365 35.83361958912467 33.047726958117785 17.288756043415177 34-35 15.332279667222254 31.470521482567783 33.631563975299436 19.565634874910526 36-37 19.053503865767397 31.54723227504524 35.006477216647475 14.392786642539892 38-39 21.863988652015706 27.57138745554345 36.54821865427708 14.016405238163765 40-41 15.215771553775115 31.183616756916187 39.63370580745147 13.966905881857224 42-43 13.86998921699336 35.51361136152676 35.6638950193611 14.952504402118775 44-45 14.81248837015646 36.5769759425442 31.58814573260561 17.02238995469373 46-47 14.064026089120008 33.631277334665754 30.048135742382275 22.256560833831962 48-49 16.89305973548461 32.39341909228221 32.032108709801136 18.68141246243205 50-51 20.289935535609636 33.73718680279243 31.95336356065513 14.019514100942803 52-53 14.63306553598197 33.59986263990476 36.075223158747555 15.69184866536572 54-55 12.021119767090418 32.95224407805596 37.1116374604467 17.914998694406922 56-57 16.056454124343375 36.69610582887189 30.25971076100217 16.98772928578256 58-59 14.221397352538274 32.90644271865812 33.080117523376735 19.792042405426873 60-61 15.235135801454575 30.164256071003 34.33886674610675 20.261741381435673 62-63 21.470626932438655 29.38139349384909 32.568992171567665 16.578987402144595 64-65 18.951843835630523 30.222350177108986 36.11789443379106 14.707911553469433 66-67 14.715711153977454 31.48852685567159 37.04145467706975 16.75430731328121 68-69 13.990181318590913 35.77247560754891 34.87153857544795 15.365804498412228 70-71 14.530741607604883 34.6291961975591 31.198402349582693 19.641659845253326 72-73 14.622306855501243 32.30570390370999 29.92477600399769 23.14721323679107 74-75 16.474368531723794 32.42988684211879 32.369631941266896 18.726112684890516 76-77 19.121969574689235 28.683725429921754 34.74997352666918 17.44433146871983 78-79 16.075218805729293 28.886776065248565 37.64691976009744 17.391085368924706 80-81 14.772074339874372 35.113574808070446 34.198356842498015 15.91599400955716 82-83 14.564853354331419 33.694940413471805 31.728322849709134 20.011883382487635 84-85 15.90461650757424 29.87051572676197 31.887365645288142 22.337502120375653 86-87 18.582721684245154 29.62314192314809 33.654474804169496 18.13966158843726 88-89 21.028286251269755 25.753525499985606 35.51984931546285 17.69833893328179 90-91 16.905201909104655 28.00596055298629 37.66666358149431 17.422173956414742 92-93 15.200307675221655 32.132830961670166 35.13986265674263 17.526998706365546 94-95 15.42871421725798 32.90130451861556 32.37593910738267 19.29404215674378 96-97 15.804801599299074 29.63125214669291 30.271917863158738 24.29202839084928 98-99 18.17893903404592 28.58191689545609 31.613042539408337 21.626101531089656 100-101 21.21104644767453 28.603855750162687 32.47533485688203 17.70976294528075 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8040.0 1 5155.5 2 3460.5 3 4466.0 4 3419.5 5 3191.0 6 6122.5 7 11833.0 8 15916.5 9 14670.0 10 9839.5 11 6247.0 12 5269.5 13 4760.0 14 4112.0 15 3253.0 16 2547.0 17 2049.5 18 1776.0 19 1599.0 20 1469.5 21 1429.0 22 1418.5 23 1536.0 24 1618.5 25 1662.5 26 1916.5 27 2232.0 28 2409.5 29 2695.0 30 3315.0 31 3930.5 32 4948.5 33 6488.0 34 7648.5 35 8256.5 36 9664.5 37 10910.0 38 10654.0 39 9971.5 40 8882.5 41 7600.0 42 6317.0 43 5844.0 44 6627.0 45 7302.5 46 11053.0 47 20387.5 48 70315.0 49 73011.0 50 29026.5 51 18229.5 52 6651.0 53 2642.5 54 1550.0 55 996.5 56 624.5 57 411.0 58 301.5 59 256.5 60 247.0 61 240.5 62 211.0 63 165.5 64 127.0 65 106.0 66 91.5 67 87.5 68 86.0 69 86.5 70 80.0 71 64.5 72 58.0 73 67.5 74 68.5 75 45.0 76 36.5 77 35.5 78 23.5 79 18.5 80 17.5 81 11.5 82 7.5 83 5.5 84 5.5 85 5.5 86 2.5 87 2.0 88 2.5 89 1.5 90 0.5 91 0.5 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2565983907263866 2 0.10124569090961259 3 0.11128827968404785 4 0.11169818126667787 5 0.11948631133664807 6 0.04898323912428626 7 0.05533671365505142 8 0.05984563106398153 9 0.05738622156820147 10-11 0.05297977955492886 12-13 0.05707879538122897 14-15 0.05277482876361385 16-17 0.054721861281106406 18-19 0.058410975524776504 20-21 0.06158771279015908 22-23 0.0699906952340743 24-25 0.07747139911707199 26-27 0.07859862846930452 28-29 0.07204020314722435 30-31 0.06927336746447177 32-33 0.0624075159554191 34-35 0.21530080627641304 36-37 0.32833116768663845 38-39 0.3057865806419879 40-41 0.28703358323666484 42-43 0.30916826869868547 44-45 0.32085046380364074 46-47 0.3256668073995434 48-49 0.33540196998700617 50-51 0.3293559216432135 52-53 0.32976582322584347 54-55 0.3182885789122032 56-57 0.2948217133066351 58-59 0.28293456741036477 60-61 0.24225183533433622 62-63 0.30537667905935784 64-65 0.33601682236095115 66-67 0.33939851041764874 68-69 0.349646049983399 70-71 0.350055951566029 72-73 0.33632424854792364 74-75 0.3390910842306762 76-77 0.3245395780473108 78-79 0.30219994179397525 80-81 0.30342964654186527 82-83 0.31326728452498553 84-85 0.3216702669689008 86-87 0.3139846122945881 88-89 0.3295608724345285 90-91 0.3536425904140416 92-93 0.34739159127893393 94-95 0.3461618865310439 96-97 0.350260902357344 98-99 0.3416529691221138 100-101 0.3192108574731207 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 487922.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.19721594845078 #Duplication Level Percentage of deduplicated Percentage of total 1 73.3747273832366 12.618410319682244 2 10.040639263964533 3.4534208336578387 3 4.3904706288955895 2.2651161456134385 4 2.519396012346709 1.7330638913596847 5 1.5886257731590174 1.36599702411451 6 1.2036849444040567 1.242001795368932 7 0.8330453229093423 1.0028242219043206 8 0.6709649739598851 0.9230983640827837 9 0.5124599268254896 0.7931595623890705 >10 4.134240665482845 14.162304630658179 >50 0.4171185450905147 4.984813146363558 >100 0.23239461797900104 8.301941703796919 >500 0.02621887997711807 2.955390410762376 >1k 0.041711854509051474 16.396268255991735 >5k 0.008342370901810295 11.30467574735306 >10k+ 0.0059588363584359245 16.49751394690135 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 22361 4.582904644594833 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 18818 3.856763990965769 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15348 3.145584745102701 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13567 2.7805673857706767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10401 2.1316931804673698 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9604 1.9683473997893104 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9587 1.9648632363369556 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8626 1.7679055258832355 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7929 1.6250548243366767 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7860 1.6109132197359415 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6206 1.2719246109009228 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5346 1.0956669303700182 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4353 0.8921507945942179 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4178 0.8562844061140921 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4100 0.8402982443915216 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4058 0.8316903111562914 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4048 0.8296408032431414 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3860 0.7911100544759204 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3805 0.779837760953595 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3567 0.7310594726206238 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3359 0.6884297080271027 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3342 0.6849455445747477 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3118 0.6390365673201864 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3081 0.6314533880415313 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2672 0.5476285143936941 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2219 0.45478580592799667 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2110 0.4324461696746611 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2059 0.42199367931759585 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1909 0.39125106062034504 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1890 0.38735699558535996 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1740 0.35661437688810915 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1648 0.33775890408712866 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1614 0.3307905771824185 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1508 0.30906579330302797 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1473 0.30189251560700275 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1459 0.29902320452859266 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1298 0.26602612712687684 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1282 0.26274691446583676 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1218 0.2496300638216764 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1204 0.2467607527432663 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1198 0.24553104799537628 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1191 0.24409639245617126 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1159 0.23753796713409112 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1122 0.22995478785543588 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1099 0.2252409196551908 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1047 0.21458347850681053 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1013 0.20761515160210034 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 924 0.18937453117506486 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 920 0.18855472800980486 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 893 0.18302105664429968 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 830 0.17010915679145439 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 769 0.15760715852123905 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 741 0.1518685363644189 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 691 0.14162099679866863 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 667 0.13670217780710853 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 663 0.1358823746418485 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 656 0.13444771910264347 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 656 0.13444771910264347 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 615 0.12604473665872826 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 591 0.12112591766716811 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 579 0.11866650817138805 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 578 0.11846155738007304 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 556 0.11395263997114292 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 546 0.11190313205799289 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 518 0.10616450990117274 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 516 0.10575460831854272 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 507 0.10391005119670767 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 503 0.10309024803144766 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 501 0.10268034644881764 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 490 0.10042588774435258 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0495079131500527E-4 0.0 12-13 0.0 0.0 0.0 2.0495079131500527E-4 0.0 14-15 0.0 0.0 0.0 4.0990158263001054E-4 0.0 16-17 0.0 0.0 0.0 5.123769782875132E-4 0.0 18-19 0.0 0.0 0.0 0.0010247539565750264 0.0 20-21 0.0 0.0 0.0 0.0013321801435475342 0.0 22-23 0.0 0.0 0.0 0.003689114243670095 0.0 24-25 0.0 0.0 0.0 0.010964867335352782 0.0 26-27 0.0 0.0 0.0 0.017625768053090456 0.0 28-29 0.0 0.0 0.0 0.035661437688810915 0.0 30-31 0.0 0.0 0.0 0.08034071019548206 0.0 32-33 0.0 0.0 0.0 0.13946901348986107 0.0 34-35 0.0 0.0 0.0 0.2570082923090166 0.0 36-37 0.0 0.0 0.0 0.428347153848361 0.0 38-39 0.0 0.0 0.0 0.6060394899184707 0.0 40-41 0.0 0.0 0.0 0.797770955193658 0.0 42-43 0.0 0.0 0.0 0.9766929960116577 0.0 44-45 0.0 0.0 0.0 1.1582793971167522 0.0 46-47 0.0 0.0 0.0 1.4113936243907839 0.0 48-49 0.0 0.0 2.0495079131500527E-4 1.7204594176938117 0.0 50-51 0.0 0.0 2.0495079131500527E-4 1.9913018884165914 0.0 52-53 0.0 0.0 2.0495079131500527E-4 2.2353982808727624 0.0 54-55 0.0 0.0 2.0495079131500527E-4 2.474678329733031 0.0 56-57 0.0 0.0 2.0495079131500527E-4 2.712216296867122 0.0 58-59 0.0 0.0 2.0495079131500527E-4 3.1138173724488754 0.0 60-61 0.0 0.0 2.0495079131500527E-4 3.7287722217895483 0.0 62-63 0.0 0.0 2.0495079131500527E-4 4.223625907419629 0.0 64-65 0.0 0.0 2.0495079131500527E-4 4.564254122585167 0.0 66-67 0.0 0.0 2.0495079131500527E-4 4.942183381770037 0.0 68-69 0.0 0.0 2.0495079131500527E-4 5.355159226269773 0.0 70-71 0.0 0.0 2.0495079131500527E-4 5.902070412893864 0.0 72-73 0.0 0.0 2.0495079131500527E-4 6.52307131057833 0.0 74-75 0.0 0.0 2.0495079131500527E-4 7.1105627538827925 0.0 76-77 0.0 0.0 2.0495079131500527E-4 7.604596636347613 0.0 78-79 0.0 0.0 2.0495079131500527E-4 8.132139973192437 0.0 80-81 0.0 0.0 2.0495079131500527E-4 8.701493271465521 0.0 82-83 0.0 0.0 2.0495079131500527E-4 9.317370399367112 0.0 84-85 0.0 0.0 2.0495079131500527E-4 10.363029336656268 0.0 86-87 0.0 0.0 2.0495079131500527E-4 11.530633994777855 0.0 88-89 0.0 0.0 2.0495079131500527E-4 12.27183853156857 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGCAT 25 5.3031094E-5 75.910645 5 TATAACG 25 5.3031094E-5 75.910645 2 GGCATTC 25 5.3247342E-5 75.84835 7 CATTCCG 25 5.3274423E-5 75.840576 9 TAACGCA 30 1.308067E-4 63.258873 4 CTTTAGG 30 1.308067E-4 63.258873 2 GCTTATG 170 0.0 61.46141 1 CTTATGT 180 0.0 57.987297 2 AGGTTGT 25 0.005061439 56.880432 9 TTAGGCA 35 2.80258E-4 54.221886 4 ATAACGC 35 2.80258E-4 54.221886 3 TCAATAC 45 1.5252519E-5 52.715725 3 CCTTTAG 40 5.389062E-4 47.49291 1 TATGTAC 225 0.0 46.38984 4 AAAGTAC 130 0.0 43.7946 3 AGGCATT 45 9.714633E-4 42.137974 6 TTATGTA 265 0.0 39.3876 3 GACAATG 85 3.6923302E-7 39.039593 7 GCTTTGC 50 0.0016160847 37.994328 1 GGAATGA 75 7.0095048E-6 37.92418 8 >>END_MODULE