##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939346_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 487922 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01072097589369 33.0 31.0 34.0 30.0 34.0 2 32.1407069162694 33.0 31.0 34.0 30.0 34.0 3 32.1911412069962 34.0 31.0 34.0 30.0 34.0 4 35.74911768684339 37.0 35.0 37.0 33.0 37.0 5 35.725042117387616 37.0 35.0 37.0 33.0 37.0 6 35.85988744102541 37.0 35.0 37.0 35.0 37.0 7 35.81748722131816 37.0 35.0 37.0 35.0 37.0 8 35.8739593623571 37.0 35.0 37.0 35.0 37.0 9 37.41600091817954 39.0 37.0 39.0 34.0 39.0 10-11 37.34381929898632 39.0 37.0 39.0 34.0 39.0 12-13 37.23997380728887 39.0 37.0 39.0 33.5 39.0 14-15 38.08259209463807 40.0 37.0 41.0 33.0 41.0 16-17 38.077765503502604 40.0 37.5 41.0 33.0 41.0 18-19 38.444578231766556 40.0 38.0 41.0 34.0 41.0 20-21 38.49601883087871 40.0 38.0 41.0 34.0 41.0 22-23 38.45270248113428 40.0 38.0 41.0 34.0 41.0 24-25 38.30518505006948 40.0 37.0 41.0 34.0 41.0 26-27 37.79726062772328 40.0 36.5 41.0 33.0 41.0 28-29 37.47683236254975 39.0 35.5 41.0 33.0 41.0 30-31 37.15989645886023 39.0 35.0 41.0 33.0 41.0 32-33 36.74624837576498 38.0 35.0 41.0 32.0 41.0 34-35 36.55749074647177 38.0 35.0 41.0 31.0 41.0 36-37 36.2394327371998 38.0 35.0 41.0 31.0 41.0 38-39 35.89900537380974 38.0 35.0 40.0 30.5 41.0 40-41 35.57891322793397 38.0 35.0 40.0 30.0 41.0 42-43 35.48966330684003 38.0 35.0 40.0 30.0 41.0 44-45 35.2764796422379 38.0 35.0 40.0 28.5 41.0 46-47 34.806558220371286 38.0 35.0 40.0 24.5 41.0 48-49 34.45873828193851 38.0 34.0 40.0 23.0 41.0 50-51 34.31043076557319 37.0 34.0 40.0 23.0 41.0 52-53 33.88005459889081 37.0 33.0 40.0 23.0 41.0 54-55 33.71785244362829 37.0 33.0 40.0 23.0 41.0 56-57 33.69504142055493 36.5 33.0 40.0 22.5 41.0 58-59 33.54359713232853 36.0 33.0 40.0 22.0 41.0 60-61 33.139530498727254 36.0 33.0 39.5 21.0 41.0 62-63 32.552360828165156 35.5 32.5 39.0 18.5 40.0 64-65 31.868394948372895 35.0 31.0 39.0 12.5 40.0 66-67 31.470538323748467 35.0 31.0 38.0 7.5 40.0 68-69 31.22510975114875 35.0 31.0 37.0 3.5 39.0 70-71 30.79771971749583 35.0 31.0 37.0 2.0 39.0 72-73 30.20279265948246 35.0 30.5 36.0 2.0 38.5 74-75 29.681226712466337 34.5 30.0 35.5 2.0 37.0 76-77 27.527243083935545 32.0 26.0 34.5 2.0 36.0 78-79 28.395167670242373 33.5 29.0 35.0 2.0 36.0 80-81 28.585225712306475 34.0 29.0 35.0 2.0 36.0 82-83 28.339527629416175 34.0 29.0 35.0 2.0 36.0 84-85 28.018543947598182 34.0 29.0 35.0 2.0 35.0 86-87 27.3831626776411 34.0 27.0 35.0 2.0 35.0 88-89 26.667625153200717 33.0 25.5 35.0 2.0 35.0 90-91 26.046237923274624 33.0 23.5 35.0 2.0 35.0 92-93 25.788370887149995 33.0 23.5 35.0 2.0 35.0 94-95 25.500437569939457 33.0 21.5 35.0 2.0 35.0 96-97 24.788928353302374 32.0 12.5 35.0 2.0 35.0 98-99 24.036453572497244 32.0 2.0 35.0 2.0 35.0 100-101 20.982002246260674 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13799773905648038 1101 2 0.04164628909421708 1101 3 -0.012988778917119248 1101 4 0.08703231291097069 1101 5 0.20263303747677952 1101 6 0.18468242589458583 1101 7 0.21113948766140567 1101 8 0.19800879459237564 1101 9 0.28971217281485906 1101 10-11 0.31664059847617665 1101 12-13 0.08394617879488209 1101 14-15 0.13766881477351234 1101 16-17 0.3699746857900408 1101 18-19 0.20019205477007063 1101 20-21 0.4270202175590043 1101 22-23 0.29325296173322357 1101 24-25 0.13606217375848217 1101 26-27 0.21903372945978106 1101 28-29 0.46198528182128484 1101 30-31 0.15193582561163055 1101 32-33 -0.09478153079406582 1101 34-35 -0.09887656812073686 1101 36-37 -0.08173580750333542 1101 38-39 0.025433823640433673 1101 40-41 0.25838238255693824 1101 42-43 -0.11071396101939968 1101 44-45 -0.008907300381444827 1101 46-47 0.14953003972561163 1101 48-49 0.07983303822968679 1101 50-51 0.07044694636254434 1101 52-53 -0.15463258886717313 1101 54-55 -0.030712379547189528 1101 56-57 -0.11644674594785442 1101 58-59 -0.27198585250925333 1101 60-61 -0.06189998797201923 1101 62-63 0.07953107404288318 1101 64-65 0.11700791528757648 1101 66-67 -0.03531424615691847 1101 68-69 0.1668320511336141 1101 70-71 0.43099914258722904 1101 72-73 0.03213105517445669 1101 74-75 0.19959407490713232 1101 76-77 0.3325840915291671 1101 78-79 0.3759942537786074 1101 80-81 0.44849550082555467 1101 82-83 0.38581745827343283 1101 84-85 0.34660392360524384 1101 86-87 0.2551779256422435 1101 88-89 0.22923423143750554 1101 90-91 0.23972304927318433 1101 92-93 0.4526361671765393 1101 94-95 0.7042790565834522 1101 96-97 0.4740990482643248 1101 98-99 0.28578488012161785 1101 100-101 0.07405879988602848 1102 1 0.11381827780797238 1102 2 0.18240201217962948 1102 3 0.12566771337548488 1102 4 0.03317248636566461 1102 5 0.1440384495082867 1102 6 0.25605627284993915 1102 7 0.24575381146392772 1102 8 0.25641950833084337 1102 9 0.0990345962772139 1102 10-11 0.2743801712750482 1102 12-13 0.14113872575942565 1102 14-15 0.20763295929865677 1102 16-17 0.19460463890211344 1102 18-19 0.1530896061782414 1102 20-21 0.14479625874682966 1102 22-23 0.10911058178776756 1102 24-25 -0.11488018167040792 1102 26-27 0.1967543019215583 1102 28-29 0.15487701054550485 1102 30-31 0.11289319743597304 1102 32-33 -0.02619644299412016 1102 34-35 0.08983968329662417 1102 36-37 0.12563601683547887 1102 38-39 0.02528825654166411 1102 40-41 0.3180648930530836 1102 42-43 0.3175367821389443 1102 44-45 -0.04941326097107179 1102 46-47 -0.2509250489937145 1102 48-49 -0.253806752153686 1102 50-51 -0.3054992397794649 1102 52-53 -0.1315180658675814 1102 54-55 -0.360138385492462 1102 56-57 -0.2572331147382698 1102 58-59 -0.3050065996515414 1102 60-61 -0.20631787280545666 1102 62-63 -0.07748219554738256 1102 64-65 0.1591993896524464 1102 66-67 0.024605768553019658 1102 68-69 -0.053709764930861326 1102 70-71 -0.10780598137464636 1102 72-73 -0.10386692220901494 1102 74-75 -0.25076754441191795 1102 76-77 -0.03940113724101835 1102 78-79 -0.09826335401766428 1102 80-81 0.034594609035536905 1102 82-83 -0.10264443379239552 1102 84-85 0.005103509888225943 1102 86-87 0.18810213881376825 1102 88-89 0.5784267171106343 1102 90-91 0.6352518409130354 1102 92-93 0.6999623294148982 1102 94-95 0.7809852562095756 1102 96-97 0.8176144170853838 1102 98-99 0.7256809911816546 1102 100-101 0.33556626211519713 1103 1 0.08396228904787506 1103 2 0.11927336000987765 1103 3 0.0813307717782692 1103 4 0.1052579351634435 1103 5 -0.08717782280388064 1103 6 -0.054190557944835405 1103 7 -0.004892802196387436 1103 8 -0.053348349382218885 1103 9 0.04954071116689107 1103 10-11 0.05085939980907739 1103 12-13 0.1063253656035954 1103 14-15 0.25605713785981976 1103 16-17 0.31257570892797304 1103 18-19 0.26251898958021513 1103 20-21 0.16425054874906664 1103 22-23 0.07924661014298096 1103 24-25 0.10616589554005174 1103 26-27 0.26095266064046285 1103 28-29 0.5446732873280808 1103 30-31 0.4568278016445433 1103 32-33 0.36694466247575264 1103 34-35 0.3295976422326632 1103 36-37 0.5584919736640401 1103 38-39 0.546115602711474 1103 40-41 0.5154497000269416 1103 42-43 0.5493038734950773 1103 44-45 1.0272623465264559 1103 46-47 1.0416077606623872 1103 48-49 1.09319327977785 1103 50-51 0.8785381839839843 1103 52-53 0.8948972995888838 1103 54-55 1.2126974544666709 1103 56-57 1.0902458902749856 1103 58-59 0.7176967244216428 1103 60-61 0.6070742145592334 1103 62-63 0.9488932554856007 1103 64-65 0.560969993477844 1103 66-67 0.926200748915118 1103 68-69 0.7118329115707169 1103 70-71 0.632646488529879 1103 72-73 0.6017482388855981 1103 74-75 0.3965246610994626 1103 76-77 0.40633919548561437 1103 78-79 0.412242122241242 1103 80-81 0.4681291110631882 1103 82-83 0.5643894455702494 1103 84-85 0.7611625193718936 1103 86-87 0.62170848680368 1103 88-89 1.011035204513643 1103 90-91 1.137119835931717 1103 92-93 1.271185626665595 1103 94-95 0.964639501507655 1103 96-97 0.5764754190970685 1103 98-99 0.6479037455961887 1103 100-101 0.5008199581908137 1104 1 0.1525092975948823 1104 2 0.28708998782651207 1104 3 0.31461748506498566 1104 4 0.12821053811604344 1104 5 0.18756965194359765 1104 6 0.08226631851204047 1104 7 0.10657572927214432 1104 8 0.17090961487574674 1104 9 0.21824311986929956 1104 10-11 0.08775916670884243 1104 12-13 0.14946444874267684 1104 14-15 0.19385822566090383 1104 16-17 0.24465030100257223 1104 18-19 0.17362233068356403 1104 20-21 0.24858621308472806 1104 22-23 0.18484878574515307 1104 24-25 0.0585901379642948 1104 26-27 0.21885475854256242 1104 28-29 0.3512544839092868 1104 30-31 0.31978817460491626 1104 32-33 0.19518864071972786 1104 34-35 0.28248808512311285 1104 36-37 0.34727985245191917 1104 38-39 0.5814069780028532 1104 40-41 0.6216113161885559 1104 42-43 0.9855276497188541 1104 44-45 0.9190494483135652 1104 46-47 0.6927888018434274 1104 48-49 0.7636361702207424 1104 50-51 0.695383560829363 1104 52-53 0.5549050695966571 1104 54-55 0.8057976875669084 1104 56-57 0.6529343129634029 1104 58-59 0.5439127306376506 1104 60-61 0.6108815183665399 1104 62-63 0.6341768607692089 1104 64-65 0.4848006073084612 1104 66-67 0.5530228157371901 1104 68-69 0.6154226551604616 1104 70-71 0.6550707309541188 1104 72-73 0.49292151005887064 1104 74-75 0.8194151039899076 1104 76-77 0.7047541139005311 1104 78-79 0.7776422776413945 1104 80-81 0.8150055679396466 1104 82-83 0.9698517610325652 1104 84-85 1.0118462800556536 1104 86-87 0.8584217735169624 1104 88-89 1.015199928678367 1104 90-91 1.1617663005781829 1104 92-93 1.174985160465127 1104 94-95 1.3145773414454993 1104 96-97 1.327462210083862 1104 98-99 1.4619519196443633 1104 100-101 1.1195845269553821 1105 1 -0.022912158343814326 1105 2 0.09958040928244571 1105 3 0.040307140237395345 1105 4 0.052559197119876444 1105 5 0.041544747729041376 1105 6 0.10172034150054543 1105 7 0.1975527407663975 1105 8 0.08140769916693813 1105 9 0.11461285167006707 1105 10-11 0.1532476724559686 1105 12-13 0.35493379740167796 1105 14-15 0.42204309156128517 1105 16-17 0.1298658182439496 1105 18-19 0.2722047061624906 1105 20-21 0.3961149487169138 1105 22-23 0.23736794283328067 1105 24-25 0.3489493333665976 1105 26-27 0.153394988427614 1105 28-29 0.48454950306636846 1105 30-31 0.3556502435704374 1105 32-33 0.168344770987936 1105 34-35 0.19777544144495351 1105 36-37 0.21565628923352875 1105 38-39 0.3520822653591651 1105 40-41 0.42770805948357093 1105 42-43 0.16460810948896665 1105 44-45 0.40335500386911605 1105 46-47 0.4239047022265723 1105 48-49 0.47932582539315405 1105 50-51 0.280048311787219 1105 52-53 -0.04151255492442374 1105 54-55 0.31792412434850803 1105 56-57 0.32303967694807767 1105 58-59 0.01294529768745889 1105 60-61 0.10317079039718635 1105 62-63 0.12086747379603224 1105 64-65 -0.14260839652296298 1105 66-67 -0.2542233528451838 1105 68-69 -0.19508021840275802 1105 70-71 -0.2107769701953046 1105 72-73 -0.2936254248070256 1105 74-75 -0.15304179256181527 1105 76-77 -0.02797577115693528 1105 78-79 -0.15481940818235884 1105 80-81 -0.07607201826725074 1105 82-83 0.21905195260344712 1105 84-85 0.1839631382932012 1105 86-87 0.5643125781146665 1105 88-89 0.4254537600959942 1105 90-91 0.11553545766630435 1105 92-93 0.2072840110398424 1105 94-95 0.6635332801428149 1105 96-97 0.22282619475819132 1105 98-99 0.5046626476137135 1105 100-101 0.5236075154611264 1106 1 -0.07965444509517283 1106 2 0.020306362095517727 1106 3 0.011459590328136926 1106 4 -0.07518127474945402 1106 5 0.09751117241143703 1106 6 0.09244175710853142 1106 7 0.07324239593881288 1106 8 0.14786867920323488 1106 9 0.23672265203303766 1106 10-11 0.2121451316211207 1106 12-13 0.11975684640349726 1106 14-15 0.2994137812164581 1106 16-17 0.24991345889730354 1106 18-19 0.031224669864847954 1106 20-21 0.0868318271198163 1106 22-23 0.08104761615451395 1106 24-25 0.09139715550815453 1106 26-27 0.21745124977062602 1106 28-29 0.153769103792321 1106 30-31 0.07189343776281731 1106 32-33 0.19513016118757065 1106 34-35 0.05283896231609475 1106 36-37 -0.09780786684632403 1106 38-39 0.2280736446695144 1106 40-41 0.42623119922948405 1106 42-43 0.340615369017101 1106 44-45 0.30249974071121954 1106 46-47 0.35308119950424555 1106 48-49 0.1899519596944259 1106 50-51 0.5442724497182496 1106 52-53 0.5988231982516226 1106 54-55 0.7296573366897121 1106 56-57 0.5086068275832858 1106 58-59 0.6366612686493411 1106 60-61 0.4953259628109805 1106 62-63 0.6409604865001981 1106 64-65 0.3750345254371119 1106 66-67 0.6748941607664243 1106 68-69 0.5403349358622158 1106 70-71 0.39577248534008547 1106 72-73 0.4110501650296321 1106 74-75 0.25730984083201136 1106 76-77 0.346157622672461 1106 78-79 0.8368820437232678 1106 80-81 0.5197424100449091 1106 82-83 0.5449979598629753 1106 84-85 0.5599749350264105 1106 86-87 0.49719370334152657 1106 88-89 0.8054923263391878 1106 90-91 0.7837546046717492 1106 92-93 0.7362132306405655 1106 94-95 1.007267399925027 1106 96-97 0.6694505141774307 1106 98-99 1.1775074751999206 1106 100-101 0.9018652287097666 1107 1 0.0701427752283621 1107 2 0.18720542794194728 1107 3 0.2015438919913919 1107 4 0.07112540103090481 1107 5 0.0773243416982865 1107 6 0.11230960855532857 1107 7 -0.003727301030885144 1107 8 0.15261235657848715 1107 9 0.04338020500638606 1107 10-11 0.0439496428993138 1107 12-13 0.13246589174411838 1107 14-15 0.22841811022078673 1107 16-17 0.11568926204152774 1107 18-19 0.2742572513184882 1107 20-21 0.21711435161287085 1107 22-23 0.21336249425886677 1107 24-25 0.055300094674251454 1107 26-27 0.25820540789320745 1107 28-29 0.21491970757450218 1107 30-31 0.2561951010118477 1107 32-33 0.2784498239809139 1107 34-35 0.4532240158590426 1107 36-37 0.34853526370733334 1107 38-39 0.013398319994188057 1107 40-41 -0.0981866636094253 1107 42-43 -0.17230784811664535 1107 44-45 -0.17145560219149303 1107 46-47 -0.3649023958477713 1107 48-49 -0.5304330938485222 1107 50-51 -0.3210955156497164 1107 52-53 -0.1157587110671301 1107 54-55 -0.6267131349439197 1107 56-57 -0.30560825557916615 1107 58-59 -0.2962892895643705 1107 60-61 -0.5072281497431277 1107 62-63 -0.5850727784804306 1107 64-65 -0.44569001318216195 1107 66-67 -0.3713350486206757 1107 68-69 -0.5218500721122687 1107 70-71 -0.5080461521627662 1107 72-73 -0.4228937857564219 1107 74-75 -0.3207580561832515 1107 76-77 -0.37762683848042045 1107 78-79 -0.2761528161537008 1107 80-81 -0.3498862069521316 1107 82-83 -0.23306310188229773 1107 84-85 -0.047778539569165446 1107 86-87 -0.19222757713238892 1107 88-89 -0.06422287074443389 1107 90-91 -0.07716587835399835 1107 92-93 0.010612866092831297 1107 94-95 0.10941139627955465 1107 96-97 -0.009521916900265381 1107 98-99 -0.0586397109472685 1107 100-101 -0.19989599252513912 1108 1 0.05282675791234226 1108 2 -0.02547237045013162 1108 3 0.12931136261600074 1108 4 -0.1546012532671739 1108 5 -0.16639860202465684 1108 6 0.024987406947410307 1108 7 -0.059818828550987746 1108 8 -0.10972530575917006 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-0.7274448437174819 2316 66-67 -0.6465435799267674 2316 68-69 -0.6938224087187486 2316 70-71 -0.6888084792665516 2316 72-73 -0.5842019897475552 2316 74-75 -0.7520483106442413 2316 76-77 -0.7132830521854139 2316 78-79 -0.7295896232490442 2316 80-81 -0.9582696933843948 2316 82-83 -0.9765168026530695 2316 84-85 -0.8603643277647102 2316 86-87 -0.9658523263668926 2316 88-89 -1.521962285800921 2316 90-91 -1.2885743885429939 2316 92-93 -0.9052355131701795 2316 94-95 -0.7080049272343345 2316 96-97 -1.0254917194554345 2316 98-99 -1.089391479503913 2316 100-101 -0.1963852755997877 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 12.0 5 62.0 6 169.0 7 270.0 8 426.0 9 663.0 10 1060.0 11 1485.0 12 1906.0 13 2625.0 14 3070.0 15 3980.0 16 4952.0 17 6454.0 18 6184.0 19 4422.0 20 4127.0 21 4465.0 22 4910.0 23 5453.0 24 6089.0 25 6816.0 26 7795.0 27 8879.0 28 10593.0 29 12082.0 30 14363.0 31 17101.0 32 20294.0 33 25540.0 34 35995.0 35 67646.0 36 62866.0 37 82109.0 38 50024.0 39 3026.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.33710145999498 18.244800848136688 14.267721906499506 15.15037578536882 2 13.030976262599351 26.518992789831163 38.89207701230934 21.557953935260144 3 24.676690126700578 34.30302384397506 29.79677079533204 11.223515233992318 4 10.92182766917663 17.95942794135128 38.32559302511467 32.79315136435742 5 9.543328646791906 45.41504584749202 31.68641709125639 13.355208414459687 6 21.896942544095165 38.210205729604326 19.277671431089395 20.61518029521112 7 21.556724230512252 42.45145740507704 19.13277122162969 16.85904714278102 8 22.706088268206802 30.854931730891412 21.514709318292677 24.924270682609105 9 27.37671185148446 22.841765692057336 21.459782506220257 28.321739950237944 10-11 21.725091305577532 28.280339890392312 25.189067104988094 24.80550169904206 12-13 32.70297301617882 27.352527658109288 24.485573513799334 15.458925811912561 14-15 28.101321342124518 27.272997440117397 30.995074941639185 13.630606276118899 16-17 15.54127725856698 32.32138465322184 34.053123462862764 18.08421462534842 18-19 14.662384561466792 34.31460356368436 36.70258770869114 14.320424166157705 20-21 16.58318337767102 32.088223117629454 32.92585700173388 18.402736502965638 22-23 15.301113702600006 31.23573030115469 30.884034743258145 22.579121252987157 24-25 17.338836945249444 32.129418226683775 33.57678071495034 16.954964113116443 26-27 20.58409404859013 27.951725494857744 35.84311298209945 15.621067474452675 28-29 16.102881198224306 28.68030135964355 39.540131414447394 15.676686027684752 30-31 14.82911203020155 36.42651899278983 35.59728809112932 13.1470808858793 32-33 13.770541193059547 35.407503658371624 33.59543123696 17.226523911608822 34-35 15.255288122080016 31.113044529774474 34.17406301783425 19.457604330311256 36-37 18.99017790063516 31.28005026356599 35.311795026325846 14.417976809473002 38-39 21.703502231040257 27.303751468059335 37.082102691390865 13.91064360950954 40-41 15.33708250954974 30.76733071567449 39.833408665332215 14.062178109443558 42-43 13.770971275846769 35.17845375943202 36.12168079696741 14.928894167753809 44-45 14.823992121635069 36.09424524792563 31.9819805971694 17.0997820332699 46-47 14.102883353162344 33.37186412158513 30.314610034605977 22.21064249064655 48-49 16.89829521931784 32.02509827390443 32.251363947516204 18.825242559261522 50-51 20.15957468611786 33.409950770819925 32.081767167703035 14.348707375359176 52-53 14.707371260160434 33.41886613024213 36.2368370354278 15.636925574169641 54-55 11.971278196105114 32.71352798193154 37.052028807883225 18.26316501408012 56-57 16.066399957370237 36.39270211222286 30.221838736519363 17.319059193887547 58-59 14.206412725023851 32.80288489620747 32.98037282713065 20.010329551638023 60-61 15.226525111955977 30.296875480360306 34.16455735117797 20.312042056505746 62-63 21.197765193670236 29.491901286489302 32.47980701635729 16.830526503483174 64-65 18.742749865757233 30.421665758051493 35.79762748963974 15.037956886551541 66-67 14.49627194510598 31.77218899742172 36.735687261488515 16.995851795983786 68-69 13.789951436760703 35.66718348583427 34.57305032274725 15.969814754657778 70-71 14.335180623132384 34.62407925857002 30.957817028131547 20.08292309016605 72-73 14.5102085989154 32.215395083640416 29.577063547042354 23.69733277040183 74-75 16.33006915039699 32.44217313423047 31.981546230749995 19.246211484622542 76-77 18.57114539865518 28.82337897892593 34.72029792710194 17.885177695316955 78-79 15.514979853337213 28.834936731690718 37.38271690966999 18.26736650530208 80-81 14.03214286080274 35.076463035095216 34.07434279091359 16.817051313188454 82-83 13.924766663524087 33.848545464234036 31.604129348543413 20.62255852369846 84-85 15.229688351826725 30.062387020876287 31.462508351744745 23.245416275552238 86-87 17.54457590572537 29.921813933163328 33.2567748606458 19.276835300465507 88-89 19.80475675319921 26.18538339085259 35.40385509261354 18.606004763334663 90-91 15.675502797691095 28.390111568280425 37.25489994436497 18.679485689663505 92-93 13.44251745155988 32.4452473961002 35.103561634851474 19.00867351748845 94-95 13.724324789618011 33.28349613257856 32.20514754407467 20.78703153372875 96-97 14.26518992789831 30.473825734441164 29.812859432450267 25.448124905210257 98-99 16.256491816314902 29.146564409885183 31.33800074602088 23.25894302777903 100-101 18.453294693198284 29.596697006277655 32.60589316713157 19.344115133392496 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7552.0 1 4882.5 2 3268.0 3 4298.0 4 3438.5 5 3194.5 6 6169.5 7 11944.5 8 15693.0 9 14377.5 10 9867.5 11 6230.0 12 5221.5 13 4715.0 14 4043.0 15 3212.0 16 2439.0 17 1986.0 18 1732.5 19 1547.5 20 1471.5 21 1441.5 22 1472.5 23 1538.0 24 1551.0 25 1632.5 26 1873.0 27 2193.5 28 2424.0 29 2712.5 30 3319.0 31 3888.0 32 4854.5 33 6409.0 34 7646.0 35 8450.0 36 9876.5 37 10900.5 38 10672.0 39 10187.5 40 9064.5 41 7735.0 42 6566.5 43 6159.0 44 6919.0 45 7441.5 46 10833.0 47 20492.0 48 70627.5 49 73803.5 50 31282.0 51 19678.5 52 6050.5 53 1522.0 54 679.0 55 445.5 56 321.5 57 241.5 58 194.5 59 159.5 60 135.0 61 127.5 62 130.0 63 104.0 64 64.0 65 47.5 66 52.0 67 53.0 68 46.0 69 46.0 70 50.0 71 50.0 72 42.0 73 43.5 74 46.5 75 35.5 76 18.5 77 15.5 78 16.5 79 12.0 80 8.5 81 6.0 82 5.0 83 1.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24758055590852635 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0018445571218350474 16-17 4.099015826300105E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.19803165260021E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002561884891437566 36-37 0.019162898987952995 38-39 0.006763376113395174 40-41 0.01670348949217293 42-43 0.0046113928045876185 44-45 7.173277696025183E-4 46-47 1.0247539565750262E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.0742618697250786E-4 60-61 0.0014346555392050367 62-63 0.0010247539565750262 64-65 0.0 66-67 0.0 68-69 3.0742618697250786E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.22370378872032826 78-79 0.0 80-81 0.0013321801435475342 82-83 0.0 84-85 0.0 86-87 0.10145064170092762 88-89 0.350055951566029 90-91 0.35200298408352154 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.0495079131500524E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 487922.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.989850836814082 #Duplication Level Percentage of deduplicated Percentage of total 1 71.2989310159194 11.400592717688482 2 10.719321182291267 3.4280069355347784 3 4.738650055115486 2.2731092264747237 4 2.642979825168551 1.6904341267661636 5 1.7983029557281653 1.437729801074762 6 1.2266400061524263 1.1768274437307602 7 0.9215821989797226 1.0315173326884215 8 0.7216283421774462 0.9230983640827837 9 0.6037068368837961 0.8687864043843073 >10 4.520751621420698 14.415418857932211 >50 0.455023199774411 4.980099278163313 >100 0.26275987592606836 8.619000577961232 >500 0.028198620831090263 3.008062764130332 >1k 0.046143197723602244 16.971975028795587 >5k 0.007690532953933707 9.449256233578318 >10k+ 0.007690532953933707 18.326084907013826 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 22032 4.515475834252196 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 19146 3.923987850517091 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15003 3.074876722099024 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 12220 2.5044986698693643 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10973 2.2489250330995527 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10043 2.0583207971765978 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9606 1.9687573013719408 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9208 1.8871868864285686 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7795 1.597591418300466 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7556 1.5486081791761797 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6676 1.3682514828189751 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5264 1.0788609654821877 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4603 0.9433884924229693 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4331 0.8876418771852879 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4166 0.8538249966183119 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3904 0.8001278892937805 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3897 0.7986932337545756 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3709 0.7601624849873545 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3676 0.7533991088739593 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3657 0.7495050438389742 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3647 0.7474555359258241 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3632 0.7443812740560991 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3312 0.6787970208352975 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3114 0.6382167641549263 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2578 0.5283631400100836 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2212 0.45335115038879165 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2168 0.4443333155709314 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1991 0.40805702550817546 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1980 0.40580256680371046 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1959 0.4014986001860953 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1949 0.3994490922729453 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1916 0.39268571615955006 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1684 0.3451371325744689 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1558 0.3193133328687782 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1543 0.31623907099905313 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1491 0.3055816298506729 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1437 0.29451428711966254 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1368 0.2803726825189272 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1267 0.25967265259611166 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1213 0.24860530986510138 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1212 0.24840035907378638 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1175 0.2408171797951312 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1163 0.2383577702993511 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1152 0.23610331159488607 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1078 0.22093695303757568 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1045 0.2141735769241805 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1014 0.20782010239341533 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1009 0.2067953484368403 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 973 0.19941711994950012 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 915 0.18752997405322983 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 886 0.18158640110509466 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 778 0.1594517156430741 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 759 0.155557650608089 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 751 0.15391804427756897 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 699 0.14326060312918867 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 679 0.13916158730288858 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 662 0.1356774238505335 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 661 0.1354724730592185 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 641 0.13137345723291838 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 638 0.13075860485897337 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 604 0.12379027795426317 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 600 0.12297047478900316 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 599 0.12276552399768814 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 580 0.11887145896270306 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 574 0.11764175421481303 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 551 0.11292788601456791 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 550 0.1127229352232529 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 546 0.11190313205799289 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 519 0.10636946069248773 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 512 0.10493480515328268 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 499 0.10227044486618762 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 490 0.10042588774435258 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 489 0.10022093695303758 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 489 0.10022093695303758 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 488 0.10001598616172257 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0495079131500527E-4 0.0 12-13 0.0 0.0 0.0 2.0495079131500527E-4 0.0 14-15 0.0 0.0 0.0 4.0990158263001054E-4 0.0 16-17 0.0 0.0 0.0 5.123769782875132E-4 0.0 18-19 0.0 0.0 0.0 0.0010247539565750264 0.0 20-21 0.0 0.0 0.0 0.0013321801435475342 0.0 22-23 0.0 0.0 0.0 0.003689114243670095 0.0 24-25 0.0 0.0 0.0 0.010964867335352782 0.0 26-27 0.0 0.0 0.0 0.017625768053090456 0.0 28-29 0.0 0.0 0.0 0.03576391308446842 0.0 30-31 0.0 0.0 0.0 0.07993080861285205 0.0 32-33 0.0 0.0 0.0 0.13844425953328604 0.0 34-35 0.0 0.0 0.0 0.2560860137480991 0.0 36-37 0.0 0.0 0.0 0.428962006222306 0.0 38-39 0.0 0.0 0.0 0.6090112763925382 0.0 40-41 0.0 0.0 0.0 0.8014600694373282 0.0 42-43 0.0 0.0 0.0 0.9825340935641351 0.0 44-45 0.0 0.0 0.0 1.16657990416501 0.0 46-47 0.0 0.0 0.0 1.422460967121794 0.0 48-49 0.0 0.0 0.0 1.7318341866117946 0.0 50-51 0.0 0.0 0.0 2.004111312873779 0.0 52-53 0.0 0.0 0.0 2.250154737847443 0.0 54-55 0.0 0.0 0.0 2.492201622390464 0.0 56-57 0.0 0.0 0.0 2.73363365455954 0.0 58-59 0.0 0.0 0.0 3.138923844384963 0.0 60-61 0.0 0.0 0.0 3.7557232508474714 0.0 62-63 0.0 0.0 0.0 4.2529338705776745 0.0 64-65 0.0 0.0 0.0 4.59571406905202 0.0 66-67 0.0 0.0 0.0 4.977127491689245 0.0 68-69 0.0 0.0 0.0 5.390820663958584 0.0 70-71 0.0 0.0 0.0 5.939166506121881 0.0 72-73 0.0 0.0 0.0 6.561397108554236 0.0 74-75 0.0 0.0 0.0 7.151757862937108 0.0 76-77 0.0 0.0 0.0 7.6491734334586265 0.0 78-79 0.0 0.0 0.0 8.179176179799232 0.0 80-81 0.0 0.0 0.0 8.747709674907053 0.0 82-83 0.0 0.0 0.0 9.364816507556537 0.0 84-85 0.0 0.0 0.0 10.409655641680432 0.0 86-87 0.0 0.0 0.0 11.575620693471496 0.0 88-89 0.0 0.0 0.0 12.317849984218789 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTTGC 40 7.4900254E-6 59.440273 1 GTGCTAA 25 0.0050385166 56.945705 9 GCTTATG 115 0.0 49.619705 1 GGACCTT 40 5.410473E-4 47.454754 8 GGGATTC 130 0.0 43.80439 7 GGTATAT 55 4.9270297E-5 43.22929 1 ACTATAC 55 4.98706E-5 43.140686 3 AGGGGTG 55 4.98706E-5 43.140686 8 CTTATGT 135 0.0 42.182007 2 TGTAGAT 85 3.662226E-7 39.080387 5 GGGAGAT 50 0.0016224821 37.963806 7 CATGTAG 100 3.0502633E-8 37.963806 3 GATTCTG 50 0.0016224821 37.963806 9 TATGTAC 150 0.0 37.963802 4 ATGGGTT 115 2.5193003E-9 37.1385 5 TAGATAT 90 5.7316356E-7 36.909252 7 GTATAGT 65 1.3185103E-4 36.57863 1 GTAGATA 95 8.7512853E-7 34.966663 6 GGAACTA 55 0.002590253 34.51255 8 GACTGAA 55 0.002590253 34.51255 9 >>END_MODULE