FastQCFastQC Report
Fri 27 May 2016
SRR939345_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939345_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2736851
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104030.3801083800323803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79990.29227020396799097No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73890.269981814866794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54760.20008396511172877No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40720.1487841318361869No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29470.10767849619873351No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27840.10172274632415136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG146350.025.9090461
TACATGG147700.025.489162
GTATCAA206050.023.7225841
ACATGGG150650.023.699623
GAGTACT93950.021.81376612-13
AGTACTT98550.021.18022712-13
CATGGGG66250.021.041134
CATGGGA83300.020.4910934
GTACTTT99800.020.3247914-15
TCAACGC239050.020.2509144
ATCAACG239950.020.2148463
CAACGCA239800.020.1875765
TATCAAC241300.020.141052
AACGCAG244550.019.8043376
GGTATCA155450.019.0193081
AGAGTAC190900.018.88827110-11
AGTACAT112300.018.8731022
GTACACG3609.822543E-1118.4553761
TACTTTT111700.018.43526614-15
CGTATAC1851.5433283E-417.9408043
Produced by FastQC (version 0.11.5)