##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939345_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2736851 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.890337106404406 33.0 31.0 34.0 30.0 34.0 2 31.943960412897887 33.0 31.0 34.0 30.0 34.0 3 31.994880247408428 34.0 31.0 34.0 30.0 34.0 4 35.483875812018994 37.0 35.0 37.0 33.0 37.0 5 35.51915760119933 37.0 35.0 37.0 33.0 37.0 6 35.614462387612626 37.0 35.0 37.0 33.0 37.0 7 35.548471217468546 37.0 35.0 37.0 33.0 37.0 8 35.53044064145253 37.0 35.0 37.0 33.0 37.0 9 37.150381953566345 39.0 37.0 39.0 34.0 39.0 10-11 37.04432301941172 39.0 37.0 39.0 33.0 39.0 12-13 36.94382704794671 39.0 37.0 39.0 33.0 39.0 14-15 38.15973175010258 40.0 38.0 41.0 33.0 41.0 16-17 38.08513050217202 40.0 38.0 41.0 33.0 41.0 18-19 38.00865520263982 40.0 38.0 41.0 33.0 41.0 20-21 37.980139583777124 40.0 38.0 41.0 33.0 41.0 22-23 37.98050039991216 40.0 38.0 41.0 33.0 41.0 24-25 37.88955061857587 40.0 38.0 41.0 33.0 41.0 26-27 37.79666996851491 40.0 38.0 41.0 32.5 41.0 28-29 37.64385949399511 40.0 38.0 41.0 32.0 41.0 30-31 37.45278149961398 40.0 37.0 41.0 32.0 41.0 32-33 37.22353865811475 40.0 37.0 41.0 31.0 41.0 34-35 36.925399848219726 40.0 36.0 41.0 30.5 41.0 36-37 36.67543318945752 40.0 36.0 41.0 30.0 41.0 38-39 36.071484709982386 38.5 35.5 40.5 29.0 41.0 40-41 36.03170559887988 39.0 35.0 40.0 30.0 41.0 42-43 36.20090753935819 39.0 35.0 41.0 30.0 41.0 44-45 36.08585432674266 39.0 35.0 41.0 29.0 41.0 46-47 35.77902578547389 39.0 35.0 41.0 27.5 41.0 48-49 35.497810987883526 39.0 35.0 41.0 26.5 41.0 50-51 34.33330276291987 38.0 34.0 40.0 24.5 40.5 52-53 34.42987725674507 38.0 34.0 39.5 24.0 40.5 54-55 34.900031094129716 39.0 35.0 40.0 25.0 41.0 56-57 34.78974193333872 38.5 34.5 40.0 25.0 41.0 58-59 34.56618226567687 38.0 34.0 40.0 24.0 41.0 60-61 34.243879370853584 38.0 34.0 40.0 23.0 41.0 62-63 33.85208255765477 37.0 34.0 40.0 22.5 41.0 64-65 33.44935913573666 37.0 33.0 40.0 22.0 41.0 66-67 33.07249828361135 36.0 33.0 39.0 20.5 41.0 68-69 32.662318847463744 35.5 32.5 39.0 19.5 40.5 70-71 32.23615936709744 35.0 32.0 38.5 18.0 40.0 72-73 31.79676661243159 35.0 32.0 37.5 16.0 40.0 74-75 31.338541813200646 35.0 31.0 37.0 12.0 39.0 76-77 30.878877037880393 35.0 31.0 36.5 7.5 39.0 78-79 30.483639226249437 35.0 31.0 36.0 7.0 38.5 80-81 30.086156681529246 34.0 30.0 35.5 2.0 37.0 82-83 29.66670180437298 34.0 30.0 35.0 2.0 37.0 84-85 29.256036956341433 34.0 29.0 35.0 2.0 36.5 86-87 28.8429001067285 34.0 29.0 35.0 2.0 36.0 88-89 28.418626735616954 34.0 29.0 35.0 2.0 36.0 90-91 28.002593491571155 34.0 27.5 35.0 2.0 35.0 92-93 27.552231012941515 34.0 27.0 35.0 2.0 35.0 94-95 27.060888042498476 33.0 26.0 35.0 2.0 35.0 96-97 26.429898266292174 33.0 25.0 35.0 2.0 35.0 98-99 25.627277115195533 33.0 22.0 35.0 2.0 35.0 100-101 22.893607288084006 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27075301978255695 1101 2 0.3018161147988714 1101 3 0.37941444532097535 1101 4 0.24266977760037634 1101 5 0.2556505286890669 1101 6 0.3242049178198414 1101 7 0.2767540964381041 1101 8 0.20593707259438787 1101 9 0.2943359308938298 1101 10-11 0.4691997508606107 1101 12-13 0.5266942374190293 1101 14-15 0.49187797943675804 1101 16-17 0.6218223869100612 1101 18-19 0.6226085829685459 1101 20-21 0.6327795931504809 1101 22-23 0.7072154487349493 1101 24-25 0.6947547711640922 1101 26-27 0.6231398771056931 1101 28-29 0.6462133804875592 1101 30-31 0.5585190428437627 1101 32-33 0.5850124715170324 1101 34-35 0.4606658181511065 1101 36-37 0.36604458439958165 1101 38-39 0.8297185002848053 1101 40-41 0.6857688833563458 1101 42-43 0.6867585529869871 1101 44-45 0.5132286853465047 1101 46-47 0.42074103815819797 1101 48-49 0.2940249113288118 1101 50-51 0.2785196807550605 1101 52-53 0.25560967815767555 1101 54-55 0.4389610278895262 1101 56-57 0.3659163352760544 1101 58-59 0.4148106649325385 1101 60-61 0.46767671473578787 1101 62-63 0.4518848351727769 1101 64-65 0.3038796903312786 1101 66-67 0.2785088391309358 1101 68-69 0.2233740656144647 1101 70-71 0.3923194532626013 1101 72-73 0.38806935238475404 1101 74-75 0.16192125504077381 1101 76-77 0.10169386776386702 1101 78-79 0.07625129366071448 1101 80-81 0.09220514006751657 1101 82-83 -0.008356459176219744 1101 84-85 -0.050335784474466294 1101 86-87 0.03619226895328609 1101 88-89 -0.013169691215100698 1101 90-91 0.11546310599991827 1101 92-93 0.22099734846115737 1101 94-95 0.3055149009333853 1101 96-97 0.235801702604288 1101 98-99 0.2739836296666738 1101 100-101 0.056162609791869045 1102 1 0.36523995318583147 1102 2 0.31732888996704034 1102 3 0.330137684893792 1102 4 0.3052081931827999 1102 5 0.2846423713388404 1102 6 0.23112379603184507 1102 7 0.3073867149766727 1102 8 0.3233084333802978 1102 9 0.41510442137338543 1102 10-11 0.41918117229015195 1102 12-13 0.4271922490814788 1102 14-15 0.5669974982617205 1102 16-17 0.6102390953040029 1102 18-19 0.5470759310481625 1102 20-21 0.5693508302117181 1102 22-23 0.5477891258480128 1102 24-25 0.48631728523877626 1102 26-27 0.4369356578825361 1102 28-29 0.41934833707794894 1102 30-31 0.4129830163131629 1102 32-33 0.37539395480889937 1102 34-35 0.3044386687992713 1102 36-37 0.23940033123825089 1102 38-39 0.7358138444486855 1102 40-41 0.6417604392139253 1102 42-43 0.4600845939830407 1102 44-45 0.5126722389468341 1102 46-47 0.45974527314498914 1102 48-49 0.4863779419208569 1102 50-51 0.5028878768917835 1102 52-53 0.5980326019279048 1102 54-55 0.47721086031810245 1102 56-57 0.4704932373110253 1102 58-59 0.41598449279363336 1102 60-61 0.2947403600831251 1102 62-63 0.297908391872852 1102 64-65 0.2773348540478864 1102 66-67 0.3754613243541769 1102 68-69 0.42680626608551364 1102 70-71 0.42313098575290553 1102 72-73 0.3880681932939041 1102 74-75 0.23894830623417818 1102 76-77 0.36027859134387796 1102 78-79 0.27988923275744426 1102 80-81 0.09969667539263227 1102 82-83 0.05619090922391479 1102 84-85 0.020051911544353374 1102 86-87 0.1817999107399011 1102 88-89 0.2209139216247138 1102 90-91 0.32873110303794206 1102 92-93 0.30638218980480403 1102 94-95 0.38873240797403597 1102 96-97 0.31517897607819734 1102 98-99 0.40169764088932425 1102 100-101 0.33785207080800106 1103 1 0.2672486616971703 1103 2 0.24945681562519795 1103 3 0.29476604089168745 1103 4 0.2087791653332971 1103 5 0.2585507428811269 1103 6 0.26984361356780084 1103 7 0.27483118811043283 1103 8 0.2380982319812972 1103 9 0.26570970072907585 1103 10-11 0.3089291105716043 1103 12-13 0.34240293298260127 1103 14-15 0.5098493596766787 1103 16-17 0.3813863810339413 1103 18-19 0.43192419064995136 1103 20-21 0.48689121694368964 1103 22-23 0.5184001821886071 1103 24-25 0.49499091297663966 1103 26-27 0.49871826198563696 1103 28-29 0.5406301365761479 1103 30-31 0.4553690349664379 1103 32-33 0.4487227043720452 1103 34-35 0.4031894121133561 1103 36-37 0.3305189015714163 1103 38-39 0.7731860791898839 1103 40-41 0.6503396856238481 1103 42-43 0.5348707933789996 1103 44-45 0.4200864383323619 1103 46-47 0.2512675151889212 1103 48-49 0.07751656217342884 1103 50-51 0.006910901371590228 1103 52-53 0.16792955512259056 1103 54-55 0.1717436548139304 1103 56-57 0.08411547857252089 1103 58-59 0.19962043320332157 1103 60-61 0.2557659248714259 1103 62-63 0.11210953788059896 1103 64-65 0.07330230993154885 1103 66-67 0.07197824583413137 1103 68-69 0.1262371091862562 1103 70-71 0.1275519766818718 1103 72-73 0.10466763567793436 1103 74-75 -0.023096316084025403 1103 76-77 0.005911099468171699 1103 78-79 0.02553677017091971 1103 80-81 0.07928390134446417 1103 82-83 0.17192735743448395 1103 84-85 0.1705959978939795 1103 86-87 0.02171222575902121 1103 88-89 -0.013567277745163864 1103 90-91 -0.04447141353665529 1103 92-93 0.039499016610012205 1103 94-95 -0.04935849412236948 1103 96-97 -0.27409679475155 1103 98-99 -0.20853437090980975 1103 100-101 -0.4253230083484283 1104 1 0.23960331007305413 1104 2 0.2509580488588874 1104 3 0.30974662415331977 1104 4 0.21538670816653394 1104 5 0.2195989852896716 1104 6 0.33060262812630725 1104 7 0.31473137150803154 1104 8 0.3583811598187623 1104 9 0.38321427321844226 1104 10-11 0.2996088893988258 1104 12-13 0.4024402786104986 1104 14-15 0.4242804660510231 1104 16-17 0.42166272871993016 1104 18-19 0.3734641826566545 1104 20-21 0.3705257203445811 1104 22-23 0.4002472437805764 1104 24-25 0.541493658960384 1104 26-27 0.4625419027205666 1104 28-29 0.5341550371890875 1104 30-31 0.5096829872531856 1104 32-33 0.6062924309440234 1104 34-35 0.6480185149343001 1104 36-37 0.5674761013278768 1104 38-39 0.9520032518288986 1104 40-41 0.7772905417905847 1104 42-43 0.6838866342999452 1104 44-45 0.6401917269812571 1104 46-47 0.7497645699952926 1104 48-49 0.7511153696532631 1104 50-51 0.7391060511089478 1104 52-53 0.8050620595768052 1104 54-55 0.9458139671874761 1104 56-57 0.8682992721014315 1104 58-59 0.697218242143613 1104 60-61 0.8189837808003517 1104 62-63 0.7283983075479057 1104 64-65 0.6565816905880197 1104 66-67 0.6156886295248185 1104 68-69 0.6799448720219061 1104 70-71 0.7821629695812788 1104 72-73 0.6242602390149514 1104 74-75 0.46501396650764804 1104 76-77 0.6277942429503334 1104 78-79 0.5664280227404141 1104 80-81 0.5744202231580715 1104 82-83 0.5917100505778023 1104 84-85 0.49822155303589355 1104 86-87 0.4813866981329653 1104 88-89 0.43007157714662014 1104 90-91 0.34336019310350707 1104 92-93 0.29775564376256725 1104 94-95 0.4247263232208418 1104 96-97 0.4799800043938376 1104 98-99 0.5181239891197222 1104 100-101 0.2680262140482448 1105 1 0.3107013744132594 1105 2 0.31567499948586786 1105 3 0.42150162799617164 1105 4 0.3434922036160515 1105 5 0.28087396529119246 1105 6 0.3986065083743213 1105 7 0.3188502137559226 1105 8 0.3723251978587072 1105 9 0.4671427347652539 1105 10-11 0.48951179874026707 1105 12-13 0.4582512697508392 1105 14-15 0.482114531153762 1105 16-17 0.5276279766105816 1105 18-19 0.5791471895593006 1105 20-21 0.6387440940911304 1105 22-23 0.5964428874299799 1105 24-25 0.6463939802632623 1105 26-27 0.6533740023618648 1105 28-29 0.5870793699396657 1105 30-31 0.6151476698776079 1105 32-33 0.5858650245206292 1105 34-35 0.5302885505153014 1105 36-37 0.4943364123992495 1105 38-39 0.9241246772891714 1105 40-41 0.6916240524859987 1105 42-43 0.4708865365698216 1105 44-45 0.3544811686285314 1105 46-47 0.3526537319654892 1105 48-49 0.40275316370330927 1105 50-51 0.41454263933814417 1105 52-53 0.3199458451834474 1105 54-55 0.51796670248482 1105 56-57 0.455070353153495 1105 58-59 0.5718719637913168 1105 60-61 0.6300375106525564 1105 62-63 0.502688467089321 1105 64-65 0.5291072180592309 1105 66-67 0.5321942224631471 1105 68-69 0.4872495656017648 1105 70-71 0.5323697411932073 1105 72-73 0.5307472873599934 1105 74-75 0.555377836282851 1105 76-77 0.48236035134873134 1105 78-79 0.465876088830111 1105 80-81 0.5042136445933032 1105 82-83 0.5691779776011501 1105 84-85 0.6234892551592957 1105 86-87 0.6273968656858422 1105 88-89 0.5938159923524644 1105 90-91 0.6805600500072977 1105 92-93 0.672240298322766 1105 94-95 0.6598467605209315 1105 96-97 0.6950956275802511 1105 98-99 0.7720984984481234 1105 100-101 0.4398781577315596 1106 1 0.2300168691741611 1106 2 0.27308157833568814 1106 3 0.2451305967669839 1106 4 0.22635369664092764 1106 5 0.30166606506350035 1106 6 0.23047699684219936 1106 7 0.33155354010533244 1106 8 0.32703152836241145 1106 9 0.2736988817859256 1106 10-11 0.4114803765671553 1106 12-13 0.39741815493554355 1106 14-15 0.4440558281210869 1106 16-17 0.5178286324243331 1106 18-19 0.4494002637403369 1106 20-21 0.44788300896659194 1106 22-23 0.4714528036777139 1106 24-25 0.445114272266828 1106 26-27 0.5660063788602301 1106 28-29 0.4923184084833636 1106 30-31 0.3939545498351862 1106 32-33 0.4529480726266115 1106 34-35 0.2643826006801149 1106 36-37 0.3548130759706041 1106 38-39 0.8368156137377767 1106 40-41 0.7183331592505908 1106 42-43 0.5732726237641401 1106 44-45 0.6052800936640068 1106 46-47 0.6035140295596477 1106 48-49 0.4507836454555374 1106 50-51 0.21324129589211083 1106 52-53 0.4169736248122575 1106 54-55 0.4906696163183284 1106 56-57 0.6171401358441457 1106 58-59 0.6129803276811217 1106 60-61 0.7502600788496636 1106 62-63 0.6094938833366186 1106 64-65 0.6952811013022284 1106 66-67 0.4533808951415921 1106 68-69 0.4952397534926618 1106 70-71 0.5113186650892203 1106 72-73 0.43114258144777295 1106 74-75 0.491338492073524 1106 76-77 0.618040943456176 1106 78-79 0.5895080432929696 1106 80-81 0.6376201345935435 1106 82-83 0.6667439750977806 1106 84-85 0.6973203711475158 1106 86-87 0.7367210088571277 1106 88-89 0.6858163425582724 1106 90-91 0.6775445731605458 1106 92-93 0.7343519960911102 1106 94-95 0.7397632794174775 1106 96-97 0.9331744227337957 1106 98-99 0.8056529893472444 1106 100-101 0.5645212088722786 1107 1 0.14873397993561 1107 2 0.17903245660341227 1107 3 0.25234746284471044 1107 4 0.18189358036195102 1107 5 0.1420418432688919 1107 6 0.18711706530423555 1107 7 0.16004383742830441 1107 8 0.18156547791996758 1107 9 0.2118651234467066 1107 10-11 0.3390211281400326 1107 12-13 0.3466237081993384 1107 14-15 0.3861687097926918 1107 16-17 0.45576240396521683 1107 18-19 0.43077609985740395 1107 20-21 0.3847968761797702 1107 22-23 0.19496157098500788 1107 24-25 0.2909070571298642 1107 26-27 0.3510417804640227 1107 28-29 0.32942224522840746 1107 30-31 0.3502354835252106 1107 32-33 0.4389720074427572 1107 34-35 0.3843511801614383 1107 36-37 0.35078196998646405 1107 38-39 0.8507678006448529 1107 40-41 0.599132046797358 1107 42-43 0.3105504425741259 1107 44-45 0.30283612458939047 1107 46-47 0.47437811303591104 1107 48-49 0.37048525482394723 1107 50-51 0.24508585983772235 1107 52-53 0.36224612398132194 1107 54-55 0.44327857879622456 1107 56-57 0.24862675893159292 1107 58-59 0.21045653105706918 1107 60-61 0.25301417733629705 1107 62-63 0.2705986269072582 1107 64-65 0.2662053470574577 1107 66-67 0.20530755644524845 1107 68-69 0.20066950895440527 1107 70-71 0.3118697318031991 1107 72-73 0.20234285459330437 1107 74-75 0.18788621473090927 1107 76-77 0.16784416724311413 1107 78-79 0.25822673221638937 1107 80-81 0.10297817563069245 1107 82-83 0.025530659472799755 1107 84-85 0.14395221669952818 1107 86-87 0.3083094549549408 1107 88-89 0.4437218361812647 1107 90-91 0.39394584787627807 1107 92-93 0.29275977063845815 1107 94-95 0.27908620401784034 1107 96-97 0.30136448228951096 1107 98-99 0.6019895402097575 1107 100-101 0.41679879850570245 1108 1 0.16725989909523165 1108 2 0.12904006869140616 1108 3 0.15133584806710587 1108 4 0.09319134621412672 1108 5 0.1964563785509199 1108 6 0.19467602726916766 1108 7 0.2815105599169456 1108 8 0.20845042120991053 1108 9 0.41388597422441364 1108 10-11 0.48455473550853867 1108 12-13 0.4481558787862312 1108 14-15 0.4325739012367009 1108 16-17 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-1.4242704566711666 2316 64-65 -1.3589295597360191 2316 66-67 -1.5184081860574636 2316 68-69 -1.5722603421614636 2316 70-71 -1.2761109060648081 2316 72-73 -1.088804324359426 2316 74-75 -1.166409592774258 2316 76-77 -1.14461773331276 2316 78-79 -1.041387075706357 2316 80-81 -0.9445952015261021 2316 82-83 -0.8949060037464349 2316 84-85 -0.8885571754617736 2316 86-87 -0.8218885672507774 2316 88-89 -0.8373228378861306 2316 90-91 -0.9429448313830129 2316 92-93 -1.0063517094937673 2316 94-95 -0.7377659443162905 2316 96-97 -0.7564881855875107 2316 98-99 -0.922717990690245 2316 100-101 -0.16244186550277462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10431.0 3 2688.0 4 2641.0 5 3019.0 6 3554.0 7 4224.0 8 5276.0 9 6535.0 10 7442.0 11 9205.0 12 11328.0 13 14941.0 14 15454.0 15 16486.0 16 20575.0 17 25768.0 18 23104.0 19 16875.0 20 16719.0 21 17842.0 22 19076.0 23 20950.0 24 23709.0 25 26756.0 26 31186.0 27 36521.0 28 42905.0 29 51158.0 30 61533.0 31 75684.0 32 94465.0 33 122142.0 34 168459.0 35 246168.0 36 377834.0 37 565897.0 38 476979.0 39 61301.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.71060385695404 18.16089098298571 14.10807342481937 23.020431735240887 2 15.512442560269166 19.978777405806895 40.10920228960971 24.39957774431423 3 19.920629633506856 24.241856338318932 29.126808838353707 26.710705189820505 4 13.007029976063961 15.869469870537436 36.18369171493781 34.9398084384608 5 14.201753904176048 37.27104959630911 33.146628586074954 15.380567913439885 6 32.74616603210705 34.813895227982414 17.237246046606387 15.202692693304149 7 29.61388133653703 32.5382145752424 19.785133557123583 18.06277053109699 8 26.987125989826506 34.078240701951316 19.369628282942113 19.565005025280065 9 27.245880789506806 17.041156979313936 20.044433906759046 35.66852832442021 10-11 25.80018979927666 26.09350431365318 26.3628012441584 21.743504642911756 12-13 26.73287174511258 23.491889588292693 27.15228844660718 22.622950219987544 14-15 23.36226739743609 24.73899981469174 25.952393888156866 25.946338899715304 16-17 22.749467931110225 28.236457504809515 26.35361855310548 22.660456010974784 18-19 22.588089546554084 26.627994850668845 28.793300674070643 21.990614928706425 20-21 23.516911858081144 25.58753166982069 28.592994450850888 22.30256202124728 22-23 22.99499529980165 25.531821449922898 28.686709500659052 22.7864737496164 24-25 23.082918207829252 25.83940280498912 28.684476795436108 22.393202191745516 26-27 22.67755962818555 25.947095094360893 28.865231717908824 22.510113559544738 28-29 22.31245373619406 26.42012156458359 28.93476814799294 22.332656551229416 30-31 22.531631626338314 25.882976568562533 28.779825875785587 22.805565929313566 32-33 22.370039839937547 26.018419955140498 28.752188854223455 22.859351350698503 34-35 23.21638907461024 26.051115119473877 28.284360644852235 22.448135161063647 36-37 23.158616945299926 25.698354374223715 28.51717475181257 22.62585392866379 38-39 22.688237604970436 26.034007053594806 28.633260960072015 22.644494381362744 40-41 22.70567757983704 26.007699464398648 28.73913243698891 22.547490518775405 42-43 22.613653296611744 26.057421771488848 28.140866740119634 23.188058191779774 44-45 22.74303768175306 26.108594785581353 28.092745146293424 23.055622386372164 46-47 23.08180056191168 26.497389656148883 27.600621703577183 22.820188078362253 48-49 23.224377758342367 26.364343013208842 27.573025809228476 22.838253419220315 50-51 22.766839927886235 26.677009359996756 27.667408200949488 22.88874251116752 52-53 23.16831788569568 26.69195040359393 27.301847292493914 22.83788441821648 54-55 22.684104581242124 26.43990428685023 27.475175709310673 23.400815422596974 56-57 22.976437410076166 26.88959981038832 27.196122793374816 22.937839986160697 58-59 23.23411213144692 26.207960062299936 27.376588291596132 23.18133951465701 60-61 22.959359723777105 26.360148774895283 28.015990037670424 22.66450146365719 62-63 22.765134600239005 26.396755887469936 27.946130170859558 22.8919793414315 64-65 22.521228677260602 26.576270352752285 27.6536259531477 23.248875016839417 66-67 22.622703595264852 26.67853885452477 27.303333849116385 23.395423701093993 68-69 22.54046222931711 26.4816447254171 27.673877534086742 23.304015511179042 70-71 22.799633245438283 26.53896950779078 27.491356578866455 23.17004066790448 72-73 22.793092751904762 26.29212637591567 27.616015594169806 23.29876527800976 74-75 22.58471003157525 26.973728408803478 27.11341428628593 23.328147273335343 76-77 22.81299525727503 26.527768907954723 27.2169174563256 23.44231837844464 78-79 22.822123578138005 26.609406858406103 27.29652088773804 23.271948675717848 80-81 22.851837546891645 26.92899992935864 27.152795425375658 23.066367098374055 82-83 22.968763504860235 26.715249144543087 27.11866770579732 23.197319644799354 84-85 22.77711041695799 26.6608934756335 27.088616096742975 23.47338001066554 86-87 22.886499106201864 26.529903287022705 27.22556286527037 23.35803474150506 88-89 22.80456119853965 26.562743458183967 26.98841274394453 23.644282599331856 90-91 22.77793859398745 26.586688492801724 27.170913285350345 23.46445962786048 92-93 22.96546612145158 26.33878254714522 27.25853930034973 23.43721203105347 94-95 22.806824963236828 26.16801894815562 27.353306629743486 23.67184945886407 96-97 22.87581723341216 26.01460662751815 27.439034575602157 23.670541563467534 98-99 22.730500769365328 26.228483605993176 26.975877556467083 24.065138068174413 100-101 22.91714573634562 25.512664965904307 27.6733648025144 23.89682449523567 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3423.0 1 1991.5 2 709.5 3 853.5 4 730.0 5 687.5 6 1059.0 7 2069.5 8 3299.0 9 3788.0 10 3440.5 11 3158.5 12 3360.0 13 3729.0 14 4001.0 15 4192.0 16 4255.0 17 4279.5 18 4460.5 19 4710.0 20 4983.5 21 5462.0 22 6342.0 23 7551.0 24 8727.5 25 10274.5 26 12741.0 27 15681.5 28 18837.0 29 22085.5 30 25557.5 31 29914.0 32 35427.5 33 39851.5 34 43885.0 35 47948.0 36 52992.0 37 59546.5 38 64829.5 39 67990.5 40 69864.5 41 75381.5 42 81541.5 43 86088.0 44 90306.0 45 94514.0 46 97091.0 47 103335.5 48 121245.5 49 122985.0 50 108925.0 51 102578.0 52 99440.0 53 99980.5 54 100663.5 55 94463.5 56 84686.0 57 76237.5 58 69179.0 59 60667.0 60 50967.0 61 42993.5 62 36608.5 63 29026.0 64 22309.0 65 17856.5 66 13644.5 67 11479.0 68 8955.0 69 5919.5 70 4541.0 71 3586.5 72 2917.5 73 2458.0 74 1974.5 75 1652.5 76 1219.5 77 750.5 78 481.5 79 326.5 80 218.0 81 163.5 82 137.5 83 100.5 84 72.5 85 53.0 86 37.0 87 23.0 88 22.0 89 17.0 90 8.5 91 9.5 92 9.5 93 7.5 94 6.0 95 5.5 96 4.5 97 1.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2890182914597835 2 0.17746673092543216 3 0.1771744241831214 4 0.1974897427737206 5 0.2119589265181042 6 0.10720349774247849 7 0.12200152657196171 8 0.13409571803507023 9 0.13895531762598695 10-11 0.12558228416526876 12-13 0.11595443084040746 14-15 0.13035053789921336 16-17 0.10769676537012794 18-19 0.13447937063435314 20-21 0.159307174559375 22-23 0.16513504023419617 24-25 0.18806285033419795 26-27 0.16909945042678612 28-29 0.16656003560296123 30-31 0.1557081477946735 32-33 0.16597542211833965 34-35 0.3470229106370789 36-37 0.46588213972919973 38-39 0.4332533996187589 40-41 0.4237168921508698 42-43 0.44595047373788343 44-45 0.44976873055931804 46-47 0.45313025809589197 48-49 0.46507829618784513 50-51 0.45835524111469717 52-53 0.46411003010394064 54-55 0.4476495066775648 56-57 0.41217077582959394 58-59 0.4021227315626609 60-61 0.35727191578935064 62-63 0.4623744588214703 64-65 0.45994465902601206 66-67 0.46906097555182946 68-69 0.48004074755987813 70-71 0.47348211502928 72-73 0.46608310061453834 74-75 0.47068693180593313 76-77 0.4478504675629035 78-79 0.43491589421565147 80-81 0.43182840424999386 82-83 0.4434841355996362 84-85 0.44532932191047303 86-87 0.4473206615924652 88-89 0.45437256175071283 90-91 0.471326352804738 92-93 0.4762042215670491 94-95 0.47490711039804506 96-97 0.4731898082869692 98-99 0.4696090506936622 100-101 0.4525821829540593 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2736851.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.23959074830245 #Duplication Level Percentage of deduplicated Percentage of total 1 73.23612333395066 34.596444942880325 2 14.207578498159656 13.423203875548872 3 5.059424680080831 7.1701545392663855 4 2.3995725746340946 4.534193055862603 5 1.3343228227376154 3.1516432036122333 6 0.7832964172552056 2.2201561311448472 7 0.5495284623381992 1.8171649765780322 8 0.3742685196751419 1.4144233359541347 9 0.2820310742180758 1.1990729271929412 >10 1.5394671474677657 13.300093792263015 >50 0.1393651116443465 4.59950772325719 >100 0.08586826355229511 7.557099794175748 >500 0.006670899225413075 2.1158817802381398 >1k 0.00217192067803539 1.7552405166854235 >5k 2.3270578693236323E-4 0.7645213699113729 >10k+ 7.756859564412108E-5 0.38119803542887326 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10403 0.3801083800323803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7999 0.29227020396799097 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7389 0.269981814866794 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5476 0.20008396511172877 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4072 0.1487841318361869 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2947 0.10767849619873351 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2784 0.10172274632415136 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.653834278884747E-5 0.0 12-13 0.0 1.8269171394423736E-5 0.0 3.653834278884747E-5 1.8269171394423736E-5 14-15 0.0 3.653834278884747E-5 0.0 3.653834278884747E-5 3.653834278884747E-5 16-17 0.0 3.653834278884747E-5 0.0 7.307668557769495E-5 7.307668557769495E-5 18-19 0.0 3.653834278884747E-5 0.0 1.0961502836654242E-4 7.307668557769495E-5 20-21 0.0 3.653834278884747E-5 0.0 1.461533711553899E-4 7.307668557769495E-5 22-23 0.0 3.653834278884747E-5 0.0 2.1923005673308486E-4 1.0961502836654242E-4 24-25 0.0 3.653834278884747E-5 0.0 6.759593415936783E-4 1.2788419976096616E-4 26-27 0.0 3.653834278884747E-5 0.0 0.0010230735980877293 1.461533711553899E-4 28-29 0.0 3.653834278884747E-5 0.0 0.002448068966852781 1.461533711553899E-4 30-31 0.0 3.653834278884747E-5 0.0 0.007490360271713733 1.461533711553899E-4 32-33 0.0 3.653834278884747E-5 0.0 0.01636917756940367 1.461533711553899E-4 34-35 0.0 3.653834278884747E-5 0.0 0.031240283084464592 2.1923005673308484E-4 36-37 0.0 3.653834278884747E-5 0.0 0.056177702037852995 2.1923005673308484E-4 38-39 0.0 3.653834278884747E-5 0.0 0.09434200108080418 2.374992281275086E-4 40-41 0.0 3.653834278884747E-5 0.0 0.13899185596877578 2.557683995219323E-4 42-43 0.0 3.653834278884747E-5 0.0 0.18406190179881915 2.557683995219323E-4 44-45 0.0 3.653834278884747E-5 0.0 0.2378280732126082 2.7403757091635605E-4 46-47 0.0 3.653834278884747E-5 0.0 0.3079451530244065 2.923067423107798E-4 48-49 0.0 3.653834278884747E-5 0.0 0.3850410563088747 2.923067423107798E-4 50-51 0.0 3.653834278884747E-5 0.0 0.471929235460754 2.923067423107798E-4 52-53 0.0 3.653834278884747E-5 0.0 0.5608087542946254 3.105759137052035E-4 54-55 0.0 3.653834278884747E-5 0.0 0.6572699792571828 3.288450850996273E-4 56-57 0.0 3.653834278884747E-5 0.0 0.7769330518906583 3.288450850996273E-4 58-59 0.0 3.653834278884747E-5 0.0 0.9094028136716248 3.288450850996273E-4 60-61 0.0 3.653834278884747E-5 0.0 1.0601782851898038 3.288450850996273E-4 62-63 0.0 3.653834278884747E-5 0.0 1.2132922106464692 3.6538342788847476E-4 64-65 0.0 3.653834278884747E-5 0.0 1.3716676574647286 3.6538342788847476E-4 66-67 0.0 3.653834278884747E-5 0.0 1.5605708896830701 3.6538342788847476E-4 68-69 0.0 3.653834278884747E-5 0.0 1.7804220982435655 3.6538342788847476E-4 70-71 0.0 3.653834278884747E-5 0.0 2.023456885303584 3.6538342788847476E-4 72-73 0.0 3.653834278884747E-5 0.0 2.273671456721612 4.384601134661697E-4 74-75 0.0 3.653834278884747E-5 0.0 2.532691768751752 4.384601134661697E-4 76-77 0.0 3.653834278884747E-5 0.0 2.818165840961017 4.384601134661697E-4 78-79 0.0 3.653834278884747E-5 0.0 3.149075342428214 4.384601134661697E-4 80-81 0.0 3.653834278884747E-5 0.0 3.515079922144099 4.384601134661697E-4 82-83 0.0 3.653834278884747E-5 0.0 3.885761409736957 4.384601134661697E-4 84-85 0.0 3.653834278884747E-5 0.0 4.26815343619364 4.384601134661697E-4 86-87 0.0 3.653834278884747E-5 0.0 4.6766886469157445 4.384601134661697E-4 88-89 0.0 3.653834278884747E-5 0.0 5.124338153593309 4.384601134661697E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14635 0.0 25.909046 1 TACATGG 14770 0.0 25.48916 2 GTATCAA 20605 0.0 23.722584 1 ACATGGG 15065 0.0 23.69962 3 GAGTACT 9395 0.0 21.813766 12-13 AGTACTT 9855 0.0 21.180227 12-13 CATGGGG 6625 0.0 21.04113 4 CATGGGA 8330 0.0 20.491093 4 GTACTTT 9980 0.0 20.32479 14-15 TCAACGC 23905 0.0 20.250914 4 ATCAACG 23995 0.0 20.214846 3 CAACGCA 23980 0.0 20.187576 5 TATCAAC 24130 0.0 20.14105 2 AACGCAG 24455 0.0 19.804337 6 GGTATCA 15545 0.0 19.019308 1 AGAGTAC 19090 0.0 18.888271 10-11 AGTACAT 11230 0.0 18.873102 2 GTACACG 360 9.822543E-11 18.455376 1 TACTTTT 11170 0.0 18.435266 14-15 CGTATAC 185 1.5433283E-4 17.940804 3 >>END_MODULE