FastQCFastQC Report
Fri 27 May 2016
SRR939345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939345_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2736851
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107330.3921660331527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88250.322450875111579No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT79670.2911009769987478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59740.21828005982057483No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39840.14556875767076832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGG11050.023.6199113
GTACATG145750.023.2515771
GTATCAA214400.023.1769731
TACATGG145550.023.0507492
GTAAGGT11950.022.2381134
ACATGGG148150.021.9735533
TAAGGTG12800.021.502845
GAGTACT99500.021.24762512-13
CATGGGG70400.021.09844
AGTACTT104900.020.53837812-13
GGTATCA164700.020.345111
GTACTTT105000.020.24764314-15
AGGTAAG12000.019.7727282
TCAACGC250500.019.6827434
CAACGCA251500.019.6233525
TATCAAC251650.019.6116542
ATCAACG252250.019.5461923
AACGCAG256950.019.2440686
AGAGTAC199800.018.64489410-11
TACCTGG19150.018.3375282
Produced by FastQC (version 0.11.5)