##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939345_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2736851 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.288454139447126 33.0 31.0 34.0 31.0 34.0 2 32.495964888114116 34.0 31.0 34.0 31.0 34.0 3 32.58069036275632 34.0 31.0 34.0 31.0 34.0 4 36.11222678910909 37.0 35.0 37.0 35.0 37.0 5 36.055604780822925 37.0 35.0 37.0 35.0 37.0 6 36.09287790968526 37.0 35.0 37.0 35.0 37.0 7 36.034130100615634 37.0 35.0 37.0 35.0 37.0 8 36.007728590266694 37.0 35.0 37.0 35.0 37.0 9 37.70629931991183 39.0 37.0 39.0 35.0 39.0 10-11 37.62329224353098 39.0 37.0 39.0 35.0 39.0 12-13 37.54388492468169 39.0 37.0 39.0 35.0 39.0 14-15 38.81766745796538 40.0 38.0 41.0 35.0 41.0 16-17 38.823504823609326 40.0 38.0 41.0 34.5 41.0 18-19 38.93364673487888 40.0 38.0 41.0 35.0 41.0 20-21 38.9162427914417 40.0 38.0 41.0 35.0 41.0 22-23 38.83817551631419 40.0 38.0 41.0 35.0 41.0 24-25 38.760515826400486 40.0 38.0 41.0 34.0 41.0 26-27 38.596261542919216 40.0 38.0 41.0 34.0 41.0 28-29 38.42641963336696 40.0 38.0 41.0 34.0 41.0 30-31 38.222938150451014 40.0 38.0 41.0 34.0 41.0 32-33 38.165291972416476 40.0 38.0 41.0 33.5 41.0 34-35 38.05909163487526 40.0 37.5 41.0 33.0 41.0 36-37 37.891194661309655 40.0 37.0 41.0 33.0 41.0 38-39 37.75176032600971 40.0 37.0 41.0 33.0 41.0 40-41 37.55623671146146 40.0 37.0 41.0 33.0 41.0 42-43 37.349637594447046 40.0 37.0 41.0 32.0 41.0 44-45 37.079062944968506 40.0 36.0 41.0 31.0 41.0 46-47 36.72559832449775 40.0 36.0 41.0 31.0 41.0 48-49 36.374603695999525 39.0 35.5 41.0 30.0 41.0 50-51 36.0984662665231 39.0 35.0 41.0 29.5 41.0 52-53 35.82753445474379 39.0 35.0 40.0 29.0 41.0 54-55 35.55785627350558 38.5 35.0 40.0 28.0 41.0 56-57 35.222421315592264 38.0 34.5 40.0 27.5 41.0 58-59 34.894684073045994 38.0 34.0 40.0 27.0 41.0 60-61 34.53183896383106 37.5 34.0 40.0 26.0 41.0 62-63 34.17281722680555 37.0 34.0 40.0 26.0 41.0 64-65 33.84701779526909 36.5 33.0 39.0 26.0 41.0 66-67 33.41929922381598 36.0 33.0 39.0 24.5 40.0 68-69 32.942472388887815 35.0 32.5 38.5 23.0 40.0 70-71 32.44631494370721 35.0 32.0 38.0 22.0 40.0 72-73 31.946710653959606 35.0 31.0 37.0 21.0 39.0 74-75 31.42691892982117 35.0 31.0 36.5 19.5 39.0 76-77 29.507875291712995 32.5 28.5 35.0 16.0 37.0 78-79 30.472013456340882 34.0 30.0 35.0 17.5 37.0 80-81 30.490872539279632 34.0 30.0 35.0 17.5 37.0 82-83 30.141710308672266 34.0 30.0 35.0 12.5 37.0 84-85 29.6957600176261 34.0 30.0 35.0 7.0 36.0 86-87 29.099530993831962 34.0 29.0 35.0 2.0 36.0 88-89 28.505434530414696 33.5 29.0 35.0 2.0 35.0 90-91 28.007940695346583 33.0 27.0 35.0 2.0 35.0 92-93 27.641873817756245 33.0 27.0 35.0 2.0 35.0 94-95 27.091603269597066 33.0 26.0 35.0 2.0 35.0 96-97 26.326776284130922 32.5 25.0 34.0 2.0 35.0 98-99 25.28808217911753 32.0 21.5 34.0 2.0 35.0 100-101 20.961926864122308 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15054242535004647 1101 2 0.19303052304712054 1101 3 0.19233847579476304 1101 4 0.12929819057863057 1101 5 0.20070007663968426 1101 6 0.24410042644804975 1101 7 0.2087350375840913 1101 8 0.21649055867804634 1101 9 0.3533806839946436 1101 10-11 0.3708278045161393 1101 12-13 0.3689337896832967 1101 14-15 0.4122265433979635 1101 16-17 0.42291354863868946 1101 18-19 0.40608362646422336 1101 20-21 0.39207813449215223 1101 22-23 0.41385242548400214 1101 24-25 0.3652999973586901 1101 26-27 0.4121041998197228 1101 28-29 0.38037220933009763 1101 30-31 0.43781292606340116 1101 32-33 0.5045797478490286 1101 34-35 0.5588344740831417 1101 36-37 0.5578799969282286 1101 38-39 0.5633765924510001 1101 40-41 0.5272633985033508 1101 42-43 0.5912304142731841 1101 44-45 0.6240709683056451 1101 46-47 0.5663849686584754 1101 48-49 0.5813016743574337 1101 50-51 0.6838267786578172 1101 52-53 0.5753207150668231 1101 54-55 0.5645264619520631 1101 56-57 0.497013751726584 1101 58-59 0.5593998598001022 1101 60-61 0.43175236487774526 1101 62-63 0.2874768965226906 1101 64-65 0.44603810392218435 1101 66-67 0.4065934238982081 1101 68-69 0.47033579270377857 1101 70-71 0.4815858637161554 1101 72-73 0.5612865622745211 1101 74-75 0.46448910046149905 1101 76-77 0.5453675629872556 1101 78-79 0.6476851863402224 1101 80-81 0.8041452626669496 1101 82-83 0.8104035779610186 1101 84-85 0.7251234074586392 1101 86-87 0.6160743478439521 1101 88-89 0.9535832050516824 1101 90-91 1.0154480348197978 1101 92-93 0.8727079773589814 1101 94-95 0.7572342065631545 1101 96-97 0.48867084864652455 1101 98-99 0.23628192004349913 1101 100-101 0.030732226679372587 1102 1 0.1320198224161544 1102 2 0.17727549789153585 1102 3 0.19033805057399888 1102 4 0.0908075984223018 1102 5 0.13617266116058602 1102 6 0.15445435318291345 1102 7 0.20130278147667724 1102 8 0.2101201953726104 1102 9 0.26775417220900977 1102 10-11 0.2293702047217252 1102 12-13 0.27262062585541 1102 14-15 0.3641284127796851 1102 16-17 0.34676599902297767 1102 18-19 0.26408572474982606 1102 20-21 0.24653854789680452 1102 22-23 0.38411801956112157 1102 24-25 0.3114756267781331 1102 26-27 0.3783987486485785 1102 28-29 0.2916246798494555 1102 30-31 0.31021291970495923 1102 32-33 0.44347214712734484 1102 34-35 0.38393329485722916 1102 36-37 0.4279872260784998 1102 38-39 0.44145141209646965 1102 40-41 0.4382876109176834 1102 42-43 0.4721942678594715 1102 44-45 0.4720557895142292 1102 46-47 0.411296046520242 1102 48-49 0.4050164578657558 1102 50-51 0.37339111945966863 1102 52-53 0.47488193294675796 1102 54-55 0.3917637225501309 1102 56-57 0.40741859566659855 1102 58-59 0.44861859282769956 1102 60-61 0.4526391819745328 1102 62-63 0.3588744090474947 1102 64-65 0.19744118339475136 1102 66-67 0.21742988015345333 1102 68-69 0.29332432300307687 1102 70-71 0.022622233337642683 1102 72-73 0.04144665259088143 1102 74-75 0.17223725850055516 1102 76-77 0.4128693214936554 1102 78-79 0.28617808011981083 1102 80-81 0.34315049116018415 1102 82-83 0.395652226129922 1102 84-85 0.37696281840204193 1102 86-87 0.2180625860523584 1102 88-89 0.4499256110858454 1102 90-91 0.5748661778370376 1102 92-93 0.5247431918636458 1102 94-95 0.3291475145157108 1102 96-97 0.1674042305954373 1102 98-99 -0.15518304825702245 1102 100-101 -0.09776019684050397 1103 1 0.10913422497419845 1103 2 0.19360102251479816 1103 3 0.2238624938708753 1103 4 0.11102150335112526 1103 5 0.14549701473814025 1103 6 0.13578543310091362 1103 7 0.1912312016343094 1103 8 0.17457413337238847 1103 9 0.2560759906943133 1103 10-11 0.12384666383423593 1103 12-13 0.17664182465150446 1103 14-15 0.299592123403599 1103 16-17 0.2455593850924913 1103 18-19 0.14704896591221228 1103 20-21 0.14017649655858122 1103 22-23 0.2519631324666136 1103 24-25 0.2421475696707489 1103 26-27 0.21377864082891307 1103 28-29 0.26791377556222784 1103 30-31 0.31392557795261666 1103 32-33 0.24718832633646315 1103 34-35 0.35937980493238797 1103 36-37 0.3689255427459486 1103 38-39 0.32119867878713393 1103 40-41 0.26968571950935427 1103 42-43 0.28708917368027187 1103 44-45 0.2481876808182335 1103 46-47 0.3927942090999821 1103 48-49 0.35348637190213594 1103 50-51 0.4129067394879442 1103 52-53 0.36733758839329766 1103 54-55 0.25713796815153955 1103 56-57 0.4784231846530318 1103 58-59 0.3602359399527657 1103 60-61 0.43623410970354115 1103 62-63 0.48996013011431927 1103 64-65 0.5038161775264811 1103 66-67 0.40830136375620185 1103 68-69 0.3119924280476951 1103 70-71 0.2859013112630393 1103 72-73 0.38354799499004244 1103 74-75 0.18473667167364383 1103 76-77 0.42251235468458503 1103 78-79 0.41794826957361764 1103 80-81 0.5510283816087274 1103 82-83 0.6260910031304796 1103 84-85 0.5869095997216505 1103 86-87 0.49014455664320167 1103 88-89 0.5754527772595779 1103 90-91 0.7040003734147113 1103 92-93 0.5413326255887192 1103 94-95 0.5253967777219231 1103 96-97 0.209960103037524 1103 98-99 0.1535004577338306 1103 100-101 0.26659623008780997 1104 1 0.20506872476440918 1104 2 0.18149684321593895 1104 3 0.15290611211999305 1104 4 0.08975927217552027 1104 5 0.1957411713519761 1104 6 0.11250091033931398 1104 7 0.125063016199789 1104 8 0.16559723798372517 1104 9 0.2484362916506484 1104 10-11 0.19938425038159124 1104 12-13 0.17919404565495256 1104 14-15 0.2020941372910201 1104 16-17 0.2021686710122168 1104 18-19 0.15582198243543388 1104 20-21 0.22992910900944707 1104 22-23 0.2297263291876206 1104 24-25 0.24479379283811653 1104 26-27 0.2594143569199687 1104 28-29 0.20451896522870783 1104 30-31 0.25370271812042944 1104 32-33 0.32246024782784843 1104 34-35 0.3156563480930785 1104 36-37 0.3308666500789883 1104 38-39 0.34718202857603586 1104 40-41 0.5293435080555327 1104 42-43 0.46936792931573734 1104 44-45 0.36459597641582775 1104 46-47 0.34041803983134145 1104 48-49 0.41524703865761836 1104 50-51 0.4894411536179035 1104 52-53 0.4300253592720438 1104 54-55 0.5125732853247982 1104 56-57 0.2726194537009192 1104 58-59 0.2610312854138357 1104 60-61 0.3573887052887983 1104 62-63 0.4316129558442654 1104 64-65 0.3883738942325934 1104 66-67 0.41503045574510367 1104 68-69 0.21924653146003692 1104 70-71 0.3307559067237875 1104 72-73 0.3971845340669269 1104 74-75 0.3158203547655809 1104 76-77 0.34162551585907863 1104 78-79 0.5203560011582233 1104 80-81 0.43319884799641883 1104 82-83 0.45920883702181214 1104 84-85 0.5086918620735972 1104 86-87 0.371796921700291 1104 88-89 0.5771261402834824 1104 90-91 0.5414108690364507 1104 92-93 0.44504203813122345 1104 94-95 0.30539607426202053 1104 96-97 0.12695572540032884 1104 98-99 0.13289095019311148 1104 100-101 0.02476492475259562 1105 1 0.15587186129822328 1105 2 0.15663266999472114 1105 3 0.16040026339416613 1105 4 0.12276038340326778 1105 5 0.15215142312167274 1105 6 0.056164921400920775 1105 7 0.1727336005173612 1105 8 0.057715846161926265 1105 9 0.1860633558979501 1105 10-11 0.21447571250875797 1105 12-13 0.21328149964034537 1105 14-15 0.25898362864793256 1105 16-17 0.15420338813175505 1105 18-19 0.23065912905354224 1105 20-21 0.1890807813408415 1105 22-23 0.11469073008384356 1105 24-25 0.19238887761598278 1105 26-27 0.2504402276531792 1105 28-29 0.32290121792021154 1105 30-31 0.281870260135328 1105 32-33 0.20973050661963555 1105 34-35 0.28416341784222254 1105 36-37 0.25083849535651126 1105 38-39 0.3343044253048433 1105 40-41 0.4326387929991782 1105 42-43 0.4527958859235639 1105 44-45 0.2666269532465435 1105 46-47 0.29213015703992795 1105 48-49 0.36989000395860216 1105 50-51 0.46866279348117246 1105 52-53 0.39201633311053996 1105 54-55 0.4849554752759673 1105 56-57 0.4004338702218959 1105 58-59 0.48213996734812525 1105 60-61 0.5236626416290804 1105 62-63 0.5731456357129403 1105 64-65 0.5686978937354965 1105 66-67 0.579876898790765 1105 68-69 0.5777067631407675 1105 70-71 0.4098150551686146 1105 72-73 0.4124711548124971 1105 74-75 0.4623514480278743 1105 76-77 0.6107973616192197 1105 78-79 0.6877156769837676 1105 80-81 0.7648508611767113 1105 82-83 0.7279666871037946 1105 84-85 0.9147956816324125 1105 86-87 1.0672609467578162 1105 88-89 1.0286839705781325 1105 90-91 1.0962226902795997 1105 92-93 0.81957404957409 1105 94-95 1.0825443875727423 1105 96-97 1.0704232204806416 1105 98-99 0.9818439106317136 1105 100-101 0.7355574979868464 1106 1 0.07836733444098343 1106 2 0.11310387624026674 1106 3 0.13224634130543933 1106 4 0.11818967832127214 1106 5 0.055705204378050155 1106 6 0.16176798359590805 1106 7 0.10275633504780046 1106 8 0.06594294222591657 1106 9 0.14502033106593615 1106 10-11 0.16479069580763195 1106 12-13 0.14340462265691656 1106 14-15 0.17012842375012838 1106 16-17 0.1031304573389491 1106 18-19 0.1203344905746988 1106 20-21 0.15632781173114552 1106 22-23 0.18327315811784928 1106 24-25 0.24189907988436232 1106 26-27 0.20919173816814407 1106 28-29 0.2138857404965151 1106 30-31 0.20881491925151607 1106 32-33 0.17036747444887368 1106 34-35 0.2591295439399772 1106 36-37 0.29412699138410403 1106 38-39 0.3451683057294659 1106 40-41 0.3256089450213864 1106 42-43 0.4459528095703291 1106 44-45 0.5723423466958479 1106 46-47 0.6167637318537302 1106 48-49 0.6503134125919487 1106 50-51 0.5737254742633837 1106 52-53 0.6709363664695118 1106 54-55 0.560179337272956 1106 56-57 0.6140928776509327 1106 58-59 0.6592389593380972 1106 60-61 0.6967748693217075 1106 62-63 0.6456767496530134 1106 64-65 0.5484344201150222 1106 66-67 0.6630174602681791 1106 68-69 0.6946109581967477 1106 70-71 0.6345569658903685 1106 72-73 0.7494640523271023 1106 74-75 0.6540208335493709 1106 76-77 0.6794518357003803 1106 78-79 0.8503580057889444 1106 80-81 0.6948430176050522 1106 82-83 0.7877906524316884 1106 84-85 0.8023252392624691 1106 86-87 0.8775187749683049 1106 88-89 0.8950153310469346 1106 90-91 0.9934805557030693 1106 92-93 1.1428253355130984 1106 94-95 1.3042439934902426 1106 96-97 1.1586503482766268 1106 98-99 1.0397338110019128 1106 100-101 0.9152624077626932 1107 1 0.10736393357055363 1107 2 0.12259645188019874 1107 3 0.17890284735364048 1107 4 0.1414097929424898 1107 5 0.07025860238034198 1107 6 0.1283971311771097 1107 7 0.11252913736119297 1107 8 0.15129666596084235 1107 9 0.14759932218952798 1107 10-11 0.14444887415859853 1107 12-13 0.17737325538404036 1107 14-15 0.11961200798376836 1107 16-17 0.23591865235805187 1107 18-19 0.24566820321582128 1107 20-21 0.2654565455594451 1107 22-23 0.2982052052079993 1107 24-25 0.2800646628340644 1107 26-27 0.25207451586495466 1107 28-29 0.3242597813514223 1107 30-31 0.22183695121601943 1107 32-33 0.21185179479215321 1107 34-35 0.3080827559661685 1107 36-37 0.2754326336013335 1107 38-39 0.283307718636749 1107 40-41 0.3849179186699274 1107 42-43 0.25340504135209585 1107 44-45 0.22996195970857514 1107 46-47 0.25410190506674724 1107 48-49 0.20238719976075004 1107 50-51 0.2973403901171636 1107 52-53 0.38475700328394424 1107 54-55 0.3871363473174725 1107 56-57 0.36503908867616275 1107 58-59 0.3644195710556346 1107 60-61 0.3735369008854903 1107 62-63 0.2651392880675374 1107 64-65 0.2409217378681916 1107 66-67 0.3504794299954668 1107 68-69 0.4533698257516292 1107 70-71 0.3766089991256365 1107 72-73 0.2712531171193042 1107 74-75 0.23947960797897494 1107 76-77 0.15474131863688356 1107 78-79 0.3936248943863596 1107 80-81 0.45750522864016574 1107 82-83 0.5622458503345698 1107 84-85 0.5032802879947376 1107 86-87 0.7880183277309278 1107 88-89 0.9557500711126821 1107 90-91 0.9208088043357598 1107 92-93 0.8044160157393314 1107 94-95 1.0119592705647058 1107 96-97 0.8906233471603855 1107 98-99 0.7466681723636803 1107 100-101 0.6613606424444392 1108 1 -0.008735473955262307 1108 2 0.008369142245832961 1108 3 0.03501773279171516 1108 4 0.05793769758840028 1108 5 0.05557203010356204 1108 6 0.03460986366296481 1108 7 0.0700203834599975 1108 8 0.0830495395240618 1108 9 0.11303251343055365 1108 10-11 0.05113181166336034 1108 12-13 0.046831100275063875 1108 14-15 0.018783162610652937 1108 16-17 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-0.9800294606823172 2316 92-93 -0.8055901287271361 2316 94-95 -0.5903166318706958 2316 96-97 -0.27969005152852944 2316 98-99 -0.04955282626220381 2316 100-101 0.3497006786314607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 32.0 5 80.0 6 142.0 7 374.0 8 822.0 9 1546.0 10 2570.0 11 4024.0 12 5959.0 13 8182.0 14 10285.0 15 14023.0 16 18568.0 17 25636.0 18 24556.0 19 17015.0 20 15679.0 21 16867.0 22 17788.0 23 19283.0 24 22208.0 25 26256.0 26 31325.0 27 38225.0 28 45996.0 29 56323.0 30 68479.0 31 83957.0 32 104554.0 33 135605.0 34 185663.0 35 269334.0 36 417091.0 37 615222.0 38 411374.0 39 21795.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63305096204972 18.856445771631414 14.685517640173945 22.824985626144915 2 15.925784779661004 20.540942857320328 39.28913192570585 24.244140437312808 3 19.98168698259423 24.33413437560174 29.494371450985092 26.189807190818936 4 13.34599508705443 16.480948359994755 35.79573751000694 34.37731904294388 5 14.119073343780864 37.455491731190335 33.15967876950553 15.265756155523263 6 32.67919956183219 35.27729496417598 16.900591226924668 15.142914247067157 7 29.43744471292007 32.73254554230391 19.665374549071178 18.164635195704847 8 26.95155856128083 34.48064947635074 19.0362573629328 19.53153459943563 9 27.00771799414729 17.043675377285794 19.89604110709717 36.052565521469745 10-11 25.83105547214664 26.288186678777908 26.216681141940136 21.66407670713532 12-13 26.733771769087905 23.581207014923354 27.07211682331263 22.612904392676107 14-15 23.22668055631932 24.919838227688114 25.9100971404842 25.943384075508362 16-17 22.60522986914 28.368674862559367 26.40122580310419 22.624869465196443 18-19 22.460835913397535 26.65856246075553 28.897504631692524 21.983096994154412 20-21 23.420182538252902 25.574702459140084 28.727687404246705 22.277427598360305 22-23 22.922877423725296 25.54972850184391 28.827455349231652 22.699938725199143 24-25 22.96288690907908 25.901099475272858 28.88929649440178 22.246717121246277 26-27 22.59064252699271 25.969453934267495 29.047956592432904 22.391946946306884 28-29 22.21277300079544 26.490188906886054 29.053189230981154 22.243848861337355 30-31 22.444663593304863 25.905429268893336 28.907054128997157 22.742853008804644 32-33 22.365137890224933 25.980405948296053 28.898613771812936 22.755842389666082 34-35 23.128563799768965 26.067296999406427 28.447916615283823 22.356222585540785 36-37 23.12065994663905 25.674425131059174 28.662755186961558 22.54215973534022 38-39 22.64047426650397 26.06269755242807 28.831925623366345 22.464902557701617 40-41 22.620933110270737 25.9713846768275 28.90331069638975 22.50437151651201 42-43 22.559819412267753 26.02867210249479 28.296925488743135 23.114582996494317 44-45 22.699589484818976 26.11547041166616 28.188051395696807 22.99688870781806 46-47 23.0201831014117 26.51587010439762 27.678212568929762 22.78573422526092 48-49 23.17864207186504 26.40581459227499 27.598742125266067 22.8168012105939 50-51 22.723518410493526 26.69843555518855 27.722843364240525 22.8552026700774 52-53 23.17928258320615 26.730065655844115 27.26484162249479 22.825810138454944 54-55 22.710881958864405 26.457194783347727 27.46068382970063 23.371239428087243 56-57 22.976754739814442 26.8730707508287 27.200677721849036 22.94949678750782 58-59 23.24776256179937 26.228729789886252 27.36279996251068 23.160707685803697 60-61 22.862004065291668 26.469831670032484 27.95560333188762 22.712560932788225 62-63 22.754650301153312 26.493142386104708 27.822280083542456 22.92992722919952 64-65 22.489723587335806 26.64347698997022 27.597968467398655 23.26883095529532 66-67 22.617474285058677 26.70274364566636 27.258970496190305 23.420811573084656 68-69 22.516814363652106 26.542450901546587 27.59033644073711 23.350398294064192 70-71 22.76046449002887 26.56856365216813 27.39122444005903 23.27974741774397 72-73 22.728073249146554 26.369173915569387 27.516496148310594 23.386256686973457 74-75 22.515939857865792 27.083745912271407 26.955733781537166 23.44458044832563 76-77 22.64417430523858 26.609741569485667 27.200502163905888 23.545581961369866 78-79 22.652991801511668 26.703913921353266 27.28852847401717 23.354565803117897 80-81 22.628849913787654 27.009620925101036 27.13195147554832 23.229577685562987 82-83 22.740624169894524 26.77352548604217 27.13574834727941 23.350101996783895 84-85 22.547464220741283 26.750287100028462 27.008540106860035 23.693708572370216 86-87 22.580695307489208 26.59460709016634 27.186225455080148 23.638472147264306 88-89 22.42422908781504 26.641910923334212 26.962635331396882 23.971224657453863 90-91 22.416146210027794 26.649724804659304 27.13178379780562 23.80234518750728 92-93 22.51795950893929 26.445740012883416 27.193460659714397 23.842839818462895 94-95 22.33720432716286 26.25100891499026 27.29185841684476 24.119928341002122 96-97 22.275783372934807 26.131090073957257 27.3916629001725 24.201463652935434 98-99 22.092320104119814 26.377305709818632 26.94313931323161 24.58723487282994 100-101 22.17611067356294 25.54996334278036 27.73010420954228 24.54382177411443 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 747.0 1 526.5 2 436.0 3 584.0 4 512.5 5 490.5 6 858.5 7 1818.0 8 2958.0 9 3419.0 10 3108.5 11 2794.0 12 2970.0 13 3291.5 14 3499.5 15 3730.0 16 3864.5 17 3962.0 18 4193.5 19 4417.0 20 4786.5 21 5422.0 22 6322.0 23 7634.0 24 8946.5 25 10634.5 26 13238.5 27 16047.0 28 18884.5 29 22281.0 30 25958.0 31 30216.5 32 35837.0 33 40303.0 34 44151.0 35 48255.5 36 53753.5 37 60218.0 38 65351.0 39 68428.0 40 70095.5 41 75587.0 42 81834.0 43 86150.0 44 90233.5 45 94960.5 46 97330.0 47 103571.5 48 122127.5 49 123813.0 50 110147.5 51 103660.5 52 99546.5 53 100188.0 54 101458.0 55 95032.5 56 84556.0 57 75945.5 58 69111.0 59 60604.5 60 50633.0 61 42594.5 62 36391.0 63 28852.0 64 21984.5 65 17504.5 66 13517.5 67 11421.5 68 8649.0 69 5598.5 70 4239.0 71 3379.5 72 2788.0 73 2356.0 74 1958.0 75 1646.0 76 1144.0 77 638.0 78 383.0 79 252.5 80 168.0 81 125.5 82 78.5 83 37.0 84 24.0 85 20.5 86 17.5 87 8.5 88 5.5 89 5.5 90 2.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29004136505787126 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.00151634122573717 16-17 0.0012057653120319666 18-19 1.826917139442374E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.653834278884748E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0015528795685260178 36-37 0.007508629443108156 38-39 0.0028317215661356793 40-41 0.003672103450279171 42-43 3.47114256494051E-4 44-45 0.0016807637682869836 46-47 2.557683995219323E-4 48-49 4.567292848605934E-4 50-51 5.480751418327121E-5 52-53 1.4615337115538992E-4 54-55 0.0 56-57 0.0012788419976096615 58-59 0.0026124915094025945 60-61 8.769202269323393E-4 62-63 0.0014249953687650516 64-65 3.653834278884748E-5 66-67 6.576901701992545E-4 68-69 0.0019730705105977637 70-71 0.0 72-73 0.0 74-75 3.653834278884748E-5 76-77 0.2581433918032074 78-79 1.8269171394423738E-4 80-81 1.4615337115538992E-4 82-83 0.0 84-85 0.0 86-87 0.10630830834415173 88-89 0.3730930182169216 90-91 0.27465872274376646 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.4615337115538992E-4 100-101 3.47114256494051E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2736851.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.61762409119418 #Duplication Level Percentage of deduplicated Percentage of total 1 71.96639152746384 32.82935795899547 2 14.669561157642244 13.383810529442144 3 5.371797333053412 7.351458942999301 4 2.567497910557029 4.684926181548682 5 1.4067944134281056 3.2087309362677665 6 0.8448524129305233 2.312409587136178 7 0.5789251173739699 1.8486431866921362 8 0.4222634102337434 1.5410122812406901 9 0.29417233130070286 1.207749854457513 >10 1.6264510678102628 13.71857642670727 >50 0.15030510963065752 4.826991242133198 >100 0.09152941906939242 7.920842381200822 >500 0.006653216347433788 2.042215729934936 >1k 0.0024849362261458014 1.8983256173558036 >5k 2.404776993044324E-4 0.8324783488986058 >10k+ 8.015923310147748E-5 0.3924707949894025 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10733 0.3921660331527 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8825 0.322450875111579 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7967 0.2911009769987478 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5974 0.21828005982057483 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3984 0.14556875767076832 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 7.307668557769495E-5 14-15 0.0 0.0 0.0 0.0 9.134585697211868E-5 16-17 0.0 0.0 0.0 3.653834278884747E-5 1.461533711553899E-4 18-19 0.0 0.0 0.0 7.307668557769495E-5 1.461533711553899E-4 20-21 0.0 0.0 0.0 1.0961502836654242E-4 1.461533711553899E-4 22-23 0.0 0.0 0.0 1.8269171394423735E-4 1.8269171394423738E-4 24-25 0.0 0.0 0.0 6.394209988048309E-4 1.8269171394423738E-4 26-27 0.0 0.0 0.0 9.865352552988818E-4 1.8269171394423738E-4 28-29 0.0 0.0 0.0 0.002411530624063933 2.009608853386611E-4 30-31 0.0 0.0 0.0 0.007490360271713732 2.1923005673308484E-4 32-33 0.0 0.0 0.0 0.01633263922661482 2.1923005673308484E-4 34-35 0.0 0.0 0.0 0.031203744741675747 2.1923005673308484E-4 36-37 0.0 0.0 0.0 0.0564152012659805 2.1923005673308484E-4 38-39 0.0 0.0 0.0 0.09531026716470864 2.1923005673308484E-4 40-41 0.0 0.0 0.0 0.1403255054805687 2.1923005673308484E-4 42-43 0.0 0.0 0.0 0.18581574225268382 2.374992281275086E-4 44-45 0.0 0.0 0.0 0.24000210460854463 2.557683995219323E-4 46-47 0.0 0.0 0.0 0.3107586054191478 2.557683995219323E-4 48-49 0.0 0.0 0.0 0.38847566053102633 2.557683995219323E-4 50-51 0.0 0.0 0.0 0.47552826222545547 3.105759137052035E-4 52-53 0.0 0.0 0.0 0.5646087419446656 3.6538342788847476E-4 54-55 0.0 0.0 0.0 0.6614901578492947 3.6538342788847476E-4 56-57 0.0 0.0 0.0 0.7819753431955192 3.6538342788847476E-4 58-59 0.0 0.0 0.0 0.9149201034327408 3.6538342788847476E-4 60-61 0.0 0.0 0.0 1.067339800376418 3.6538342788847476E-4 62-63 0.0 0.0 0.0 1.2218969903732428 4.019217706773222E-4 64-65 0.0 0.0 0.0 1.3811493574184346 4.384601134661697E-4 66-67 0.0 0.0 0.0 1.571386239148569 4.5672928486059346E-4 68-69 0.0 0.0 0.0 1.7920778295932078 4.749984562550172E-4 70-71 0.0 0.0 0.0 2.034820309910916 4.749984562550172E-4 72-73 0.0 0.0 0.0 2.284797382100816 4.932676276494409E-4 74-75 0.0 0.0 0.0 2.5440369241876883 5.115367990438646E-4 76-77 0.0 0.0 0.0 2.829858110653448 5.115367990438646E-4 78-79 0.0 0.0 0.0 3.162740682631243 5.115367990438646E-4 80-81 0.0 0.0 0.0 3.5314490997135026 5.298059704382884E-4 82-83 0.0 0.0 0.0 3.904395964559269 5.480751418327121E-4 84-85 0.0 0.0 0.0 4.289035099097466 5.480751418327121E-4 86-87 0.0 0.0 0.0 4.697478963962598 5.480751418327121E-4 88-89 0.0 0.0 0.0 5.146023660038489 5.480751418327121E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTAAGG 1105 0.0 23.619911 3 GTACATG 14575 0.0 23.251577 1 GTATCAA 21440 0.0 23.176973 1 TACATGG 14555 0.0 23.050749 2 GTAAGGT 1195 0.0 22.238113 4 ACATGGG 14815 0.0 21.973553 3 TAAGGTG 1280 0.0 21.50284 5 GAGTACT 9950 0.0 21.247625 12-13 CATGGGG 7040 0.0 21.0984 4 AGTACTT 10490 0.0 20.538378 12-13 GGTATCA 16470 0.0 20.34511 1 GTACTTT 10500 0.0 20.247643 14-15 AGGTAAG 1200 0.0 19.772728 2 TCAACGC 25050 0.0 19.682743 4 CAACGCA 25150 0.0 19.623352 5 TATCAAC 25165 0.0 19.611654 2 ATCAACG 25225 0.0 19.546192 3 AACGCAG 25695 0.0 19.244068 6 AGAGTAC 19980 0.0 18.644894 10-11 TACCTGG 1915 0.0 18.337528 2 >>END_MODULE