FastQCFastQC Report
Fri 27 May 2016
SRR939344_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939344_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787708
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105370.37798076412594145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81050.2907406371112039No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT74160.2660249925745451No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56620.20310592070618586No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT41690.14954937891629969No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28540.10237801089640665No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG148450.026.3644711
TACATGG147950.026.0874792
ACATGGG149900.024.5461123
GAGTACT93500.022.19831712-13
GTATCAA206450.021.9025231
CATGGGA83000.021.1942714
CATGGGG68400.020.5190984
TATAACG1856.659093E-620.5041082
AGTACTT98600.019.96878612-13
GTACTTT102950.019.93089914-15
GGTATCA161000.019.6185911
ATGGGAG35550.019.3397585
TCAACGC239950.018.7133644
ATCAACG240500.018.6311383
CAACGCA240050.018.6265585
TATCAAC241500.018.6128922
ATGGGGA39550.018.3428845
AACGCAG247100.018.1799686
TACCTGG21050.018.0202372
AGAGTAC188550.017.85612910-11
Produced by FastQC (version 0.11.5)