FastQCFastQC Report
Fri 27 May 2016
SRR939344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939344_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787708
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109100.39136093163272484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89310.3203707131449922No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78810.2827053622545833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59550.21361634719274758No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39130.1403662076515905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG152250.024.942141
TACATGG153400.024.3133642
ACATGGG153900.023.1860663
GTATCAA235100.021.6175631
GAGTACT106650.021.42391412-13
TACCTGG22200.020.5194662
AGTACTT111300.020.50752812-13
CATGGGG67350.020.361424
CCTATAC6300.020.3362583
GTACTTT114750.019.78757914-15
AGAGTAC210050.018.80723410-11
TAAGGTG12450.018.6755985
ATCAACG274250.018.5133483
TCAACGC274550.018.4931184
CAACGCA275200.018.449445
GGTATCA184750.018.416611
TATCAAC276150.018.3859712
CATGGGA86100.018.3522324
AACGCAG280500.018.1177586
TACTTTT126300.017.84652514-15
Produced by FastQC (version 0.11.5)