##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939344_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2787708 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24884134206309 33.0 31.0 34.0 31.0 34.0 2 32.44997144607685 34.0 31.0 34.0 31.0 34.0 3 32.53688083543901 34.0 31.0 34.0 31.0 34.0 4 36.082405330830916 37.0 35.0 37.0 35.0 37.0 5 36.01761088320585 37.0 35.0 37.0 35.0 37.0 6 36.06186049614953 37.0 35.0 37.0 35.0 37.0 7 36.005839205540894 37.0 35.0 37.0 35.0 37.0 8 35.983965322049514 37.0 35.0 37.0 35.0 37.0 9 37.6825833265177 39.0 37.0 39.0 35.0 39.0 10-11 37.5978052579395 39.0 37.0 39.0 35.0 39.0 12-13 37.51276496677558 39.0 37.0 39.0 35.0 39.0 14-15 38.78934970951047 40.0 38.0 41.0 34.5 41.0 16-17 38.73729117253313 40.0 38.0 41.0 34.0 41.0 18-19 38.856027783397685 40.0 38.0 41.0 35.0 41.0 20-21 38.86167525436667 40.0 38.0 41.0 35.0 41.0 22-23 38.802705304859764 40.0 38.0 41.0 34.0 41.0 24-25 38.72801957737324 40.0 38.0 41.0 34.0 41.0 26-27 38.55235501709649 40.0 38.0 41.0 34.0 41.0 28-29 38.39453863173618 40.0 38.0 41.0 34.0 41.0 30-31 38.19767116929033 40.0 38.0 41.0 33.5 41.0 32-33 37.98929550727695 40.0 37.0 41.0 33.0 41.0 34-35 37.934735452924045 40.0 37.0 41.0 33.0 41.0 36-37 37.84039289624308 40.0 37.0 41.0 33.0 41.0 38-39 37.72965461231951 40.0 37.0 41.0 33.0 41.0 40-41 37.54960670199318 40.0 37.0 41.0 32.5 41.0 42-43 37.35681050526095 40.0 37.0 41.0 32.0 41.0 44-45 37.10857844508823 40.0 36.0 41.0 31.0 41.0 46-47 36.78148087963302 40.0 36.0 41.0 31.0 41.0 48-49 36.43442785255844 39.5 35.5 41.0 30.0 41.0 50-51 36.162558453037406 39.0 35.0 41.0 30.0 41.0 52-53 35.92115332739297 39.0 35.0 40.5 29.0 41.0 54-55 35.623448366902124 39.0 35.0 40.0 28.0 41.0 56-57 35.35985117523069 38.0 35.0 40.0 28.0 41.0 58-59 35.052544240645005 38.0 34.0 40.0 27.0 41.0 60-61 34.702771416518516 37.5 34.0 40.0 26.5 41.0 62-63 34.317081989935815 37.0 34.0 40.0 26.0 41.0 64-65 33.886159167315945 36.5 33.0 39.0 26.0 41.0 66-67 33.49353483219907 36.0 33.0 39.0 25.0 40.0 68-69 33.09940998124624 35.5 33.0 39.0 24.0 40.0 70-71 32.692545094392955 35.0 32.0 38.0 23.5 40.0 72-73 32.193154196924496 35.0 32.0 37.0 22.0 39.5 74-75 31.692770369063044 35.0 31.0 37.0 20.5 39.0 76-77 29.724039605295822 33.0 28.5 35.0 18.0 37.0 78-79 30.71572901466007 34.0 30.5 36.0 18.5 37.5 80-81 30.76852238469739 34.0 31.0 35.0 18.0 37.0 82-83 30.469153512491268 34.0 31.0 35.0 17.5 37.0 84-85 30.101651428341846 34.0 30.5 35.0 12.5 36.0 86-87 29.579648585863367 34.0 30.0 35.0 7.0 36.0 88-89 29.081252771093673 34.0 29.0 35.0 2.0 35.5 90-91 28.653661897157093 34.0 29.0 35.0 2.0 35.0 92-93 28.29279465424643 34.0 29.0 35.0 2.0 35.0 94-95 27.879496346102243 33.0 28.0 35.0 2.0 35.0 96-97 27.30734173019556 33.0 27.0 35.0 2.0 35.0 98-99 26.535761277723495 33.0 25.5 35.0 2.0 35.0 100-101 23.154068682946708 29.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16403618553303545 1101 2 0.16145462834334978 1101 3 0.16304344293103412 1101 4 0.17975782037666477 1101 5 0.20300946119370877 1101 6 0.17235557003466084 1101 7 0.10888256173466004 1101 8 0.22395351045355483 1101 9 0.2584255015643606 1101 10-11 0.17970401894623222 1101 12-13 0.31173280761748146 1101 14-15 0.4216474954738345 1101 16-17 0.35154424943799256 1101 18-19 0.32359558422682255 1101 20-21 0.2871562148464548 1101 22-23 0.315388842404694 1101 24-25 0.33492709442541013 1101 26-27 0.3537490665084846 1101 28-29 0.31817533921095276 1101 30-31 0.26287015116016477 1101 32-33 0.26724655512936835 1101 34-35 0.31842067151669795 1101 36-37 0.31858564753220264 1101 38-39 0.3239909998498618 1101 40-41 0.38251031857094375 1101 42-43 0.4724253483113827 1101 44-45 0.5058147868662672 1101 46-47 0.36865809967006413 1101 48-49 0.5136622163539641 1101 50-51 0.48884840315161426 1101 52-53 0.3711855483335924 1101 54-55 0.41415075582836636 1101 56-57 0.2990115368136017 1101 58-59 0.3126380171377363 1101 60-61 0.46819428517952844 1101 62-63 0.44211531546828553 1101 64-65 0.4025855446094013 1101 66-67 0.3993397029276764 1101 68-69 0.5148659308294867 1101 70-71 0.4459198255673158 1101 72-73 0.2750291211004523 1101 74-75 0.15552025019407623 1101 76-77 0.421300505560918 1101 78-79 0.5453892362934667 1101 80-81 0.6504564013759548 1101 82-83 0.5509778293922132 1101 84-85 0.5565486448395056 1101 86-87 0.5242098088655069 1101 88-89 0.7207407246791533 1101 90-91 0.8406555808287095 1101 92-93 0.6790737184822468 1101 94-95 0.7683253288880927 1101 96-97 0.5327254914885593 1101 98-99 0.5800282069819538 1101 100-101 0.6393868603581545 1102 1 0.12423419231235044 1102 2 0.14879775500905623 1102 3 0.16580220660362954 1102 4 0.1002274009665669 1102 5 0.09465302669790532 1102 6 0.13157825632366382 1102 7 0.13560634316923625 1102 8 0.1749757020600029 1102 9 0.24270890852045568 1102 10-11 0.2550349869880435 1102 12-13 0.24174938249255717 1102 14-15 0.3540711613189984 1102 16-17 0.3297985037859732 1102 18-19 0.2832435102496049 1102 20-21 0.3271711065970351 1102 22-23 0.20877460896758038 1102 24-25 0.2661814903341906 1102 26-27 0.28984105204921917 1102 28-29 0.2851699364927569 1102 30-31 0.28426199480571057 1102 32-33 0.1787615789662027 1102 34-35 0.17780788043926776 1102 36-37 0.22522016763551278 1102 38-39 0.2957022470228239 1102 40-41 0.3013653257548796 1102 42-43 0.2561800758291284 1102 44-45 0.31183357321089034 1102 46-47 0.2049636525318803 1102 48-49 0.2945795972338914 1102 50-51 0.20496103204718708 1102 52-53 0.3095915514033365 1102 54-55 0.278933437703337 1102 56-57 0.24215146688033684 1102 58-59 0.14357596334686207 1102 60-61 0.08178401076335007 1102 62-63 0.06988898761124318 1102 64-65 0.20328298235887132 1102 66-67 0.15176044838463554 1102 68-69 0.16834960842308533 1102 70-71 0.04364359474072188 1102 72-73 0.14607837645719002 1102 74-75 0.18368639130233433 1102 76-77 0.36528199787929694 1102 78-79 0.30687321707240756 1102 80-81 0.34617257085210085 1102 82-83 0.348989513174363 1102 84-85 0.28670756077356785 1102 86-87 0.3955460871093912 1102 88-89 0.7009244416199678 1102 90-91 0.6703083332945958 1102 92-93 0.5770596538034525 1102 94-95 0.6750752154722512 1102 96-97 0.45007460383544995 1102 98-99 0.26547453035810165 1102 100-101 0.29149338541943237 1103 1 0.10424919014734968 1103 2 0.1342291291718638 1103 3 0.12200265186272219 1103 4 0.10690581335710903 1103 5 0.037184179115811844 1103 6 0.030647755507501984 1103 7 0.09037003564442614 1103 8 0.10072931009457164 1103 9 0.1336618207843827 1103 10-11 0.1078539212255265 1103 12-13 0.14839228284068184 1103 14-15 0.2945169290749874 1103 16-17 0.15727948517579904 1103 18-19 0.17407209877071494 1103 20-21 0.23853001565697696 1103 22-23 0.2926635156721673 1103 24-25 0.266925221187023 1103 26-27 0.22722445904739885 1103 28-29 0.23245959201489086 1103 30-31 0.3231592131922909 1103 32-33 0.33135207030090186 1103 34-35 0.3366762468711002 1103 36-37 0.40066047825845885 1103 38-39 0.34877995473312495 1103 40-41 0.36080818793703173 1103 42-43 0.3771356246410491 1103 44-45 0.39195169498402294 1103 46-47 0.42368892502124567 1103 48-49 0.38568931222604874 1103 50-51 0.4350804886813435 1103 52-53 0.3308431708109296 1103 54-55 0.3436289385266065 1103 56-57 0.4111936069421702 1103 58-59 0.23020567937531666 1103 60-61 0.11005952671083463 1103 62-63 0.11258842730134688 1103 64-65 0.2742764996734408 1103 66-67 0.20954405461674241 1103 68-69 0.1645026811230963 1103 70-71 0.23097048735645132 1103 72-73 0.14300211418811415 1103 74-75 0.17121894729896425 1103 76-77 0.328704515199707 1103 78-79 0.3049929586875493 1103 80-81 0.3166701675493542 1103 82-83 0.4722353359676923 1103 84-85 0.30417488866772757 1103 86-87 0.23615222775445943 1103 88-89 0.48141768303693055 1103 90-91 0.5485427055078809 1103 92-93 0.5323820922485858 1103 94-95 0.3218967376024331 1103 96-97 0.020644914914935697 1103 98-99 -0.3284804922500193 1103 100-101 -0.3347277557145567 1104 1 0.07792928350146866 1104 2 0.11541309303255787 1104 3 0.1355772198242562 1104 4 0.11583962315208396 1104 5 0.09338190186897322 1104 6 0.10230132645980916 1104 7 0.11376985412251628 1104 8 0.07756601829610332 1104 9 0.11717070639571148 1104 10-11 0.12223231032484705 1104 12-13 0.20002939855797308 1104 14-15 0.23653093527333624 1104 16-17 0.13420750869048703 1104 18-19 0.16556953439649646 1104 20-21 0.1770774277070899 1104 22-23 0.13391361460993068 1104 24-25 0.14501358053264823 1104 26-27 0.11968150620274542 1104 28-29 0.14558962532881026 1104 30-31 0.15753818128921182 1104 32-33 0.19750376274476622 1104 34-35 0.09449471634021478 1104 36-37 0.1431293517477954 1104 38-39 0.20640036390580008 1104 40-41 0.19565528175404978 1104 42-43 0.18837278236646426 1104 44-45 0.27069319750127363 1104 46-47 0.2727153005178238 1104 48-49 0.1451646233679753 1104 50-51 0.10556933512409472 1104 52-53 0.0464440147923213 1104 54-55 0.09645463160675405 1104 56-57 0.10409966981699625 1104 58-59 0.152938295511305 1104 60-61 0.22405784084228486 1104 62-63 0.19986007357323388 1104 64-65 0.02960982212490393 1104 66-67 -0.009107228987055294 1104 68-69 0.15639740765605836 1104 70-71 -9.989729839148254E-4 1104 72-73 0.13274815906473947 1104 74-75 0.2703584275131057 1104 76-77 0.377726132444721 1104 78-79 0.46782717966321385 1104 80-81 0.4205146962222095 1104 82-83 0.3952886336208117 1104 84-85 0.449593061985734 1104 86-87 0.2098819054776193 1104 88-89 0.6188880363578946 1104 90-91 0.7212654663655478 1104 92-93 0.6619910902408819 1104 94-95 0.48631919827447945 1104 96-97 0.4726586408538118 1104 98-99 0.687492527077719 1104 100-101 0.42649347957682693 1105 1 0.10765987778449926 1105 2 0.07960917339836016 1105 3 0.0971349986284551 1105 4 0.06936545533816485 1105 5 0.10835564591037183 1105 6 0.07878426868175126 1105 7 0.11094859045828542 1105 8 0.10405815346683056 1105 9 0.1295073447062407 1105 10-11 0.10234220716949949 1105 12-13 0.13817482951294835 1105 14-15 0.15160468066004285 1105 16-17 0.08746173782062527 1105 18-19 0.1338389879466959 1105 20-21 0.12848604157483123 1105 22-23 0.131891165877434 1105 24-25 0.1433570560054136 1105 26-27 0.17577822499269047 1105 28-29 0.20638102610998743 1105 30-31 0.18204822701478918 1105 32-33 0.13931306959642598 1105 34-35 0.31323061861692736 1105 36-37 0.22397416131922654 1105 38-39 0.18758568627934125 1105 40-41 0.25399209047045446 1105 42-43 0.2567154805492535 1105 44-45 0.2936600984012898 1105 46-47 0.19844180505973696 1105 48-49 0.15356742699313486 1105 50-51 0.27169287041829904 1105 52-53 0.31631550783183826 1105 54-55 0.20085850251084736 1105 56-57 0.2863394159421304 1105 58-59 0.24743343819633878 1105 60-61 0.08592877782050579 1105 62-63 0.024860783597738134 1105 64-65 0.1059341672954659 1105 66-67 0.2818610351599915 1105 68-69 0.2806416052611098 1105 70-71 0.2959793869641274 1105 72-73 0.21997519201436688 1105 74-75 0.22997731625750006 1105 76-77 0.35652338083819757 1105 78-79 0.430483852174504 1105 80-81 0.48233458617588454 1105 82-83 0.48986367274689613 1105 84-85 0.31997771588989465 1105 86-87 0.4002938841141024 1105 88-89 0.5365778109587502 1105 90-91 0.6587691846517423 1105 92-93 0.5810090590924801 1105 94-95 0.542908225630601 1105 96-97 0.5638743672238213 1105 98-99 0.6987332612580026 1105 100-101 0.44493034943008425 1106 1 0.07442486436905682 1106 2 0.13090768059228708 1106 3 0.09890907890504508 1106 4 0.07601015308544845 1106 5 0.1340441554452454 1106 6 0.0479946480957949 1106 7 0.07704298478238059 1106 8 0.06330472757591821 1106 9 0.11761747742011863 1106 10-11 0.13681972504176088 1106 12-13 0.1537133381474547 1106 14-15 0.1036180780994016 1106 16-17 0.13053270463316125 1106 18-19 0.06228081532861296 1106 20-21 0.15866773534250456 1106 22-23 0.15215263203268847 1106 24-25 0.18228742173390344 1106 26-27 0.2400308315509463 1106 28-29 0.25152931631581055 1106 30-31 0.18950181317077153 1106 32-33 0.2694670321052044 1106 34-35 0.32214963973950006 1106 36-37 0.3466115120836406 1106 38-39 0.4287766898267833 1106 40-41 0.402631638126806 1106 42-43 0.47059119408894645 1106 44-45 0.48849668205425445 1106 46-47 0.41960363676340506 1106 48-49 0.24397961434119964 1106 50-51 0.28631228590098345 1106 52-53 0.26431543580259387 1106 54-55 0.2726288012521323 1106 56-57 0.39133392371228837 1106 58-59 0.360191618017339 1106 60-61 0.27210276445908477 1106 62-63 0.3575962742069194 1106 64-65 0.21953797043953927 1106 66-67 0.22186751779194225 1106 68-69 0.42511930320647906 1106 70-71 0.36931236015124824 1106 72-73 0.34431171264173344 1106 74-75 0.3541730197130981 1106 76-77 0.3285551457347431 1106 78-79 0.46467433350992593 1106 80-81 0.5401737088596938 1106 82-83 0.41471592276040425 1106 84-85 0.25474695219983445 1106 86-87 0.3391518604524961 1106 88-89 0.487723256009442 1106 90-91 0.39137538276817807 1106 92-93 0.38036619809091476 1106 94-95 0.4807844433750077 1106 96-97 0.5793077131622759 1106 98-99 0.5351332396492303 1106 100-101 0.5013332089910634 1107 1 -0.03408555020789095 1107 2 -0.06049114478455664 1107 3 -0.009041971545642014 1107 4 -0.015641582907960583 1107 5 -0.013069716591395775 1107 6 -0.022819909483565937 1107 7 -0.007226057878995107 1107 8 0.03310307701593729 1107 9 0.05387736106463592 1107 10-11 0.047282656618918395 1107 12-13 0.04893079562097569 1107 14-15 0.07666960794576028 1107 16-17 3.664726277818886E-4 1107 18-19 0.019147380074699072 1107 20-21 -0.018327636725260277 1107 22-23 0.026579536298832807 1107 24-25 -0.014161245573802717 1107 26-27 0.12097253620724047 1107 28-29 0.08208452983019754 1107 30-31 0.16654768434960943 1107 32-33 0.1229053989777853 1107 34-35 0.027536442177300557 1107 36-37 0.09242057010227 1107 38-39 0.07739598878843879 1107 40-41 0.07420098447461498 1107 42-43 0.004844533602081924 1107 44-45 0.012796854304646388 1107 46-47 -0.06519834446199724 1107 48-49 0.07822845735813644 1107 50-51 0.08546943498404147 1107 52-53 0.07246502385437026 1107 54-55 -0.03037783280917239 1107 56-57 0.02453127838898439 1107 58-59 -0.0863692541645662 1107 60-61 -0.029385575780402462 1107 62-63 -0.007595657003605538 1107 64-65 0.09927821131338277 1107 66-67 0.14224619194815347 1107 68-69 0.09479375688218994 1107 70-71 0.015648753407880633 1107 72-73 0.11356660863432211 1107 74-75 0.1016090484076635 1107 76-77 0.16594136708687657 1107 78-79 0.34686371209415157 1107 80-81 0.2450543441800157 1107 82-83 0.3088789172885136 1107 84-85 0.2162990735083845 1107 86-87 0.15797626881736093 1107 88-89 0.30657505841264765 1107 90-91 0.44060074023962414 1107 92-93 0.2889036608218767 1107 94-95 0.3087974762962773 1107 96-97 0.36454474768844847 1107 98-99 0.6082057992623895 1107 100-101 0.5283704330618484 1108 1 0.0604610131327874 1108 2 0.0733629499542019 1108 3 0.05167865161573104 1108 4 0.003650567659626347 1108 5 0.054287395680390205 1108 6 0.044068672453548174 1108 7 0.10534249540137353 1108 8 0.10996435650977077 1108 9 0.07646805600944617 1108 10-11 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9 1557.0 10 2569.0 11 4113.0 12 5985.0 13 8596.0 14 10334.0 15 13820.0 16 18133.0 17 25711.0 18 24365.0 19 16423.0 20 15360.0 21 16277.0 22 17033.0 23 18345.0 24 21194.0 25 25437.0 26 30603.0 27 36518.0 28 44408.0 29 54829.0 30 67192.0 31 82005.0 32 100419.0 33 129791.0 34 176760.0 35 255168.0 36 398255.0 37 629232.0 38 497655.0 39 38128.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.594606241686016 18.904917131722325 14.695351074154464 22.8051255524372 2 15.918848028559662 20.585728490932336 39.20826714993105 24.28715633057695 3 19.967048198735306 24.355384423332715 29.49526994936342 26.182297428568557 4 13.374714998844931 16.484079394255065 35.793095977053554 34.348109629846455 5 14.16457534289818 37.47221731974798 33.13941058389185 15.223796753461984 6 32.740229607979025 35.205265400823905 16.928028330083354 15.126476661113719 7 29.429517008237593 32.76114284566389 19.683087324784374 18.12625282131414 8 27.01879106420041 34.49001832329641 19.006151289876847 19.485039322626328 9 26.95253591839604 17.012506331366126 19.96726342931182 36.06769432092601 10-11 25.79689120955279 26.310969441562747 26.22806979784109 21.664069551043365 12-13 26.721790804488847 23.568662858520334 27.10622848591029 22.60331785108053 14-15 23.225116657483298 24.958172624377255 25.887056041508828 25.92965467663062 16-17 22.616818004577983 28.397623696457032 26.38050630432139 22.605051994643595 18-19 22.494787832872024 26.636362201493125 28.88641852016065 21.982431445474205 20-21 23.425982922171187 25.547941176048568 28.75588117550332 22.270194726276927 22-23 22.91118725490618 25.54198646343161 28.868428831140136 22.678397450522077 24-25 22.958881416993293 25.894808472415754 28.848510002222255 22.297800108368694 26-27 22.59816061837889 25.991578648307765 29.04833632028973 22.36192441302361 28-29 22.247505836335797 26.444932539562966 29.095891678755454 22.211669945345783 30-31 22.428586494711787 25.920128650489936 28.925429062154283 22.725855792643994 32-33 22.341256688290166 26.026613978221537 28.8689848434628 22.763144490025496 34-35 23.148028737802086 26.050650779742917 28.467158783441416 22.334161699013585 36-37 23.101009104363815 25.654321209131275 28.718159393640402 22.52651029286451 38-39 22.638090213995145 26.05760360521633 28.833999726997135 22.470306453791395 40-41 22.630925679395624 25.995357709157375 28.876742496012575 22.496974115434423 42-43 22.558419181731466 26.035265853508914 28.297075336969236 23.10923962779038 44-45 22.704935880580653 26.109279047985623 28.192384444606265 22.993400626827455 46-47 23.021521829160875 26.50840503360381 27.65784537062662 22.812227766608693 48-49 23.170755330185226 26.40771558570697 27.610208816705335 22.81132026740247 50-51 22.717659094854987 26.675570038181906 27.748099872726982 22.858670994236125 52-53 23.225226601925307 26.73866846886403 27.271633183963313 22.76447174524735 54-55 22.696405075423968 26.468141570064006 27.46575322809993 23.36970012641209 56-57 22.95916985551748 26.943608682044296 27.160597013854577 22.936624448583647 58-59 23.250656726772608 26.24783467596278 27.327671774003488 23.173836823261134 60-61 22.909032534630487 26.467304839748245 27.95038464366876 22.67327798195251 62-63 22.72091029426008 26.504159387588917 27.826679628506962 22.94825068964404 64-65 22.46124773469818 26.630066707130013 27.609114727941375 23.299570830230426 66-67 22.550335257494687 26.758541425429062 27.28930002711905 23.401823289957196 68-69 22.554253759478737 26.528175508943434 27.60806718975265 23.309503541825176 70-71 22.77096861847952 26.572640781000157 27.43469320221006 23.22169739831026 72-73 22.708762897692296 26.391106959552435 27.523148048504364 23.376982094250902 74-75 22.494967191685785 27.07769249864046 26.982201866192586 23.445138443481166 76-77 22.673740898036435 26.631072589912225 27.21028681881789 23.484899693233448 78-79 22.63475945113333 26.684842888853495 27.272565849794884 23.407831810218287 80-81 22.639017331294838 27.02371224181881 27.157515548805204 23.17975487808115 82-83 22.7149149050044 26.798090043863994 27.11932167931505 23.36767337181656 84-85 22.5168310310836 26.73709011130291 27.033659909861434 23.71241894775206 86-87 22.592371284913714 26.61766004954047 27.18974904427401 23.600219621271805 88-89 22.437153624712924 26.6858438133542 26.974743010214432 23.902259551718448 90-91 22.410318209595157 26.657984759563075 27.148371512424816 23.78332551841695 92-93 22.5453132107093 26.456052786016325 27.20223925891808 23.796394744356295 94-95 22.30504814439822 26.272214714061644 27.32636045926626 24.096376682273878 96-97 22.29230966801401 26.160881986205155 27.384629236634538 24.162179109146294 98-99 22.1008441343211 26.41291699130612 26.971781119112904 24.514457755259876 100-101 22.082692797135707 25.833773211534155 27.667737978590623 24.41579601273952 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 730.0 1 528.0 2 459.5 3 613.5 4 532.5 5 497.5 6 887.5 7 1892.5 8 3053.0 9 3544.0 10 3250.0 11 2924.0 12 2972.5 13 3193.5 14 3519.0 15 3789.5 16 3957.0 17 4126.5 18 4338.5 19 4574.0 20 4980.0 21 5675.5 22 6581.0 23 7767.0 24 9055.0 25 10597.0 26 13206.0 27 16488.0 28 19463.5 29 22664.0 30 26507.0 31 30859.0 32 36533.5 33 40969.0 34 44630.0 35 49171.5 36 55030.5 37 61477.5 38 66476.0 39 69544.5 40 71341.5 41 76921.5 42 83389.5 43 88093.5 44 92765.5 45 97416.5 46 98999.0 47 105514.0 48 124502.0 49 126222.0 50 112177.0 51 105361.5 52 101267.0 53 101847.0 54 103237.0 55 96354.0 56 85997.5 57 77507.0 58 70307.0 59 61679.0 60 51559.0 61 43354.0 62 36777.5 63 29068.0 64 22433.0 65 17931.0 66 13523.0 67 11498.0 68 8872.0 69 5572.5 70 4247.5 71 3386.0 72 2755.0 73 2391.5 74 1995.5 75 1748.0 76 1254.0 77 660.5 78 389.5 79 254.5 80 147.0 81 106.5 82 70.0 83 36.5 84 29.5 85 22.5 86 15.0 87 9.5 88 4.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27814964838498146 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0021523057651662227 16-17 6.098199667970964E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.967940688192594E-5 26-27 0.0011478964080886519 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0032463945291257186 36-37 0.02091323768486513 38-39 0.006869442567155527 40-41 0.018169047834278196 42-43 0.004986175022635082 44-45 6.815634923026372E-4 46-47 1.255511696346963E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.7935881376385186E-5 58-59 6.815634923026372E-4 60-61 0.0022599210534245337 62-63 0.0013631269846052744 64-65 0.0 66-67 0.0 68-69 7.533070178081779E-4 70-71 1.7935881376385186E-5 72-73 0.0 74-75 0.0 76-77 0.23101415212784124 78-79 0.0 80-81 0.0012196399335941928 82-83 0.0 84-85 0.0 86-87 0.10453031666157288 88-89 0.3559375659143641 90-91 0.3551483871338031 92-93 0.0 94-95 1.7935881376385186E-5 96-97 0.0 98-99 0.0 100-101 1.614229323874667E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2787708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.20002864594815 #Duplication Level Percentage of deduplicated Percentage of total 1 71.51806186390148 32.326184449510386 2 14.820290618597848 13.397551210037987 3 5.48993706806284 7.444359382226789 4 2.6013284284721423 4.703204779378405 5 1.4474507042423823 3.2712406647676753 6 0.8817161620530225 2.3912157469435242 7 0.5890026882212237 1.8636056866097843 8 0.41786314435813593 1.5109940876058972 9 0.30746079335491805 1.2507513000433417 >10 1.6732257088086577 13.902637485577053 >50 0.14873603396524077 4.67952469209541 >100 0.09507917940999516 8.032001701466218 >500 0.007068040186673558 2.13743547504281 >1k 0.0024619016380449874 1.8804191344322885 >5k 2.3824854561725685E-4 0.8172473500738744 >10k+ 7.941618187241895E-5 0.3916268541883415 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10910 0.39136093163272484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8931 0.3203707131449922 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7881 0.2827053622545833 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5955 0.21361634719274758 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3913 0.1403662076515905 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.587176275277038E-5 3.587176275277038E-5 14-15 0.0 0.0 0.0 3.587176275277038E-5 7.174352550554076E-5 16-17 0.0 0.0 0.0 5.380764412915557E-5 7.174352550554076E-5 18-19 0.0 0.0 0.0 1.0761528825831113E-4 7.174352550554076E-5 20-21 0.0 0.0 0.0 1.2555116963469632E-4 7.174352550554076E-5 22-23 0.0 0.0 0.0 3.049099833985482E-4 7.174352550554076E-5 24-25 0.0 0.0 0.0 5.022046785387853E-4 7.174352550554076E-5 26-27 0.0 0.0 0.0 7.533070178081779E-4 7.174352550554076E-5 28-29 0.0 0.0 0.0 0.002582766918199467 7.174352550554076E-5 30-31 0.0 0.0 0.0 0.007963531331115024 7.174352550554076E-5 32-33 0.0 0.0 0.0 0.018025560783267114 7.174352550554076E-5 34-35 0.0 0.0 0.0 0.03258949646089188 7.174352550554076E-5 36-37 0.0 0.0 0.0 0.056677385149377195 7.174352550554076E-5 38-39 0.0 0.0 0.0 0.09527540187135812 7.174352550554076E-5 40-41 0.0 0.0 0.0 0.13661760844392598 7.174352550554076E-5 42-43 0.0 0.0 0.0 0.18222855478407352 7.174352550554076E-5 44-45 0.0 0.0 0.0 0.23718409532131773 7.174352550554076E-5 46-47 0.0 0.0 0.0 0.308299864978685 1.0761528825831113E-4 48-49 0.0 0.0 0.0 0.3837740538105139 1.0761528825831113E-4 50-51 0.0 0.0 0.0 0.4697048614847753 1.0761528825831113E-4 52-53 0.0 0.0 0.0 0.5563351685327158 1.434870510110815E-4 54-55 0.0 0.0 0.0 0.6551977466793509 1.9729469514023708E-4 56-57 0.0 0.0 0.0 0.7745969090019471 2.1523057651662227E-4 58-59 0.0 0.0 0.0 0.9083089046628987 2.1523057651662227E-4 60-61 0.0 0.0 0.0 1.0606024734297854 2.5110233926939265E-4 62-63 0.0 0.0 0.0 1.209775198837181 3.228458647749334E-4 64-65 0.0 0.0 0.0 1.3703013371558284 3.228458647749334E-4 66-67 0.0 0.0 0.0 1.5591841039305407 3.228458647749334E-4 68-69 0.0 0.0 0.0 1.778791035503001 3.228458647749334E-4 70-71 0.0 0.0 0.0 2.023167419256249 3.228458647749334E-4 72-73 0.0 0.0 0.0 2.2743773738139 3.228458647749334E-4 74-75 0.0 0.0 0.0 2.533981320855699 3.587176275277038E-4 76-77 0.0 0.0 0.0 2.8125793662750906 3.587176275277038E-4 78-79 0.0 0.0 0.0 3.143783351771419 3.587176275277038E-4 80-81 0.0 0.0 0.0 3.5115585993941973 3.7665350890408897E-4 82-83 0.0 0.0 0.0 3.8860239307703677 4.663329157860149E-4 84-85 3.587176275277038E-5 0.0 0.0 4.27203996975293 4.663329157860149E-4 86-87 3.587176275277038E-5 0.0 0.0 4.679130669352745 4.842687971624001E-4 88-89 3.587176275277038E-5 0.0 0.0 5.122505656976986 5.022046785387853E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15225 0.0 24.94214 1 TACATGG 15340 0.0 24.313364 2 ACATGGG 15390 0.0 23.186066 3 GTATCAA 23510 0.0 21.617563 1 GAGTACT 10665 0.0 21.423914 12-13 TACCTGG 2220 0.0 20.519466 2 AGTACTT 11130 0.0 20.507528 12-13 CATGGGG 6735 0.0 20.36142 4 CCTATAC 630 0.0 20.336258 3 GTACTTT 11475 0.0 19.787579 14-15 AGAGTAC 21005 0.0 18.807234 10-11 TAAGGTG 1245 0.0 18.675598 5 ATCAACG 27425 0.0 18.513348 3 TCAACGC 27455 0.0 18.493118 4 CAACGCA 27520 0.0 18.44944 5 GGTATCA 18475 0.0 18.41661 1 TATCAAC 27615 0.0 18.385971 2 CATGGGA 8610 0.0 18.352232 4 AACGCAG 28050 0.0 18.117758 6 TACTTTT 12630 0.0 17.846525 14-15 >>END_MODULE