FastQCFastQC Report
Fri 27 May 2016
SRR939343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119330
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28170.2516684087802525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19880.1776062465939446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16760.14973242922105187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35500.033.9507331
GGTATCA24100.033.2746431
GTACATG39850.026.9106331
ATCAACG43950.026.8605373
TATCAAC44900.026.50342
TACATGG40600.026.3910482
TCAACGC44750.026.3803464
ACATGGG39850.026.1739083
CAACGCA45400.025.8982285
AACGCAG46800.025.1100046
CATGGGG23850.024.8482534
GTACCTG9450.023.5998821
TACCTGG9900.022.0291062
ACGCAGA53200.021.8220467
CGCAGAG53950.021.2551888
TAGACTG4250.021.1952695
TAAGGTG4150.020.5635765
GAGTACT27900.020.29527712-13
GTACTTT28700.019.65140214-15
AGAGTAC48200.019.462610-11
Produced by FastQC (version 0.11.5)