##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939343_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1119330 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.875300402919603 33.0 31.0 34.0 30.0 34.0 2 31.975722083746525 33.0 31.0 34.0 30.0 34.0 3 32.03012427076912 34.0 31.0 34.0 30.0 34.0 4 35.51659117507795 37.0 35.0 37.0 33.0 37.0 5 35.53991584251293 37.0 35.0 37.0 33.0 37.0 6 35.61494108082513 37.0 35.0 37.0 33.0 37.0 7 35.54543342892623 37.0 35.0 37.0 33.0 37.0 8 35.527084059214 37.0 35.0 37.0 33.0 37.0 9 37.16230691574424 39.0 37.0 39.0 34.0 39.0 10-11 37.04566526404189 39.0 37.0 39.0 33.0 39.0 12-13 36.9362900127755 39.0 37.0 39.0 33.0 39.0 14-15 38.16568259583858 40.0 38.0 41.0 33.0 41.0 16-17 38.0736038523045 40.0 38.0 41.0 33.0 41.0 18-19 37.95650701759088 40.0 38.0 41.0 33.0 41.0 20-21 37.92594989860005 40.0 38.0 41.0 33.0 41.0 22-23 37.94003645037657 40.0 38.0 41.0 33.0 41.0 24-25 37.84675609516407 40.0 38.0 41.0 33.0 41.0 26-27 37.76330930109977 40.0 38.0 41.0 32.0 41.0 28-29 37.61173603852305 40.0 38.0 41.0 32.0 41.0 30-31 37.43343652006111 40.0 37.0 41.0 32.0 41.0 32-33 37.229605210259706 40.0 37.0 41.0 31.0 41.0 34-35 36.94483798343652 40.0 36.5 41.0 30.5 41.0 36-37 36.7035204095307 40.0 36.0 41.0 30.0 41.0 38-39 36.10480331984312 38.5 35.5 40.5 28.5 41.0 40-41 36.0946910205212 39.0 35.0 40.0 30.0 41.0 42-43 36.295387865955526 39.0 36.0 41.0 30.0 41.0 44-45 36.22828745767558 39.0 36.0 41.0 29.5 41.0 46-47 35.97893650666023 39.0 35.0 41.0 28.5 41.0 48-49 35.75466350406046 39.0 35.0 41.0 27.5 41.0 50-51 34.65712569126174 38.0 34.0 40.0 25.5 40.5 52-53 34.77234551026061 38.0 34.5 39.5 26.0 40.5 54-55 35.25005762375707 39.0 35.0 40.0 26.5 41.0 56-57 35.149822661770884 39.0 35.0 40.0 26.0 41.0 58-59 34.92107466073455 38.0 34.5 40.0 26.0 41.0 60-61 34.60596874916244 38.0 34.0 40.0 26.0 41.0 62-63 34.19446588584242 37.5 34.0 40.0 24.5 41.0 64-65 33.777889451725585 37.0 33.5 40.0 23.0 41.0 66-67 33.40111137912859 36.0 33.0 39.0 22.0 41.0 68-69 32.98212100095593 36.0 33.0 39.0 21.5 40.5 70-71 32.55262344438191 35.0 32.0 38.5 20.5 40.0 72-73 32.095669730999795 35.0 32.0 37.5 19.5 40.0 74-75 31.615140307148025 35.0 31.0 37.0 18.0 39.0 76-77 31.12986652729758 35.0 31.0 36.5 14.5 39.0 78-79 30.723913859183618 35.0 31.0 36.0 10.0 38.5 80-81 30.298516969973107 34.0 30.5 35.5 7.0 37.0 82-83 29.85675761392976 34.0 30.0 35.0 4.0 37.0 84-85 29.422392413318683 34.0 29.5 35.0 2.0 36.5 86-87 28.980110869895384 34.0 29.0 35.0 2.0 36.0 88-89 28.54278988323372 34.0 29.0 35.0 2.0 36.0 90-91 28.114396111959834 34.0 28.5 35.0 2.0 35.0 92-93 27.63611580141692 34.0 27.0 35.0 2.0 35.0 94-95 27.11857763126156 33.0 26.0 35.0 2.0 35.0 96-97 26.4674546380424 33.0 25.0 35.0 2.0 35.0 98-99 25.59551517425603 33.0 22.0 35.0 2.0 35.0 100-101 22.724140333949773 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21286741932850717 1101 2 0.46647287379743574 1101 3 0.4142822369011725 1101 4 0.4671921263157728 1101 5 0.27785835486506016 1101 6 0.2789867258873855 1101 7 0.31783935055733537 1101 8 0.1931760558354938 1101 9 0.2862485490339992 1101 10-11 0.4770341676787666 1101 12-13 0.44615810567717773 1101 14-15 0.5494236254869662 1101 16-17 0.5251843258924183 1101 18-19 0.6462792800229167 1101 20-21 0.7355175920340358 1101 22-23 0.6791780106976759 1101 24-25 0.8154441446938776 1101 26-27 0.6222266791006632 1101 28-29 0.7858568477642294 1101 30-31 0.5552706206087166 1101 32-33 0.5889073842034165 1101 34-35 0.5301420842427049 1101 36-37 0.4159451658573232 1101 38-39 0.9023969539851535 1101 40-41 0.8897710163210277 1101 42-43 0.6833722282626056 1101 44-45 0.586093309157242 1101 46-47 0.5299833926139428 1101 48-49 0.6133749161563529 1101 50-51 0.5504958689772437 1101 52-53 0.525278182852837 1101 54-55 0.5922973597985717 1101 56-57 0.5219344435097 1101 58-59 0.35188260961151485 1101 60-61 0.5702954774819986 1101 62-63 0.5636991821951867 1101 64-65 0.1782776036861904 1101 66-67 0.1083825399457794 1101 68-69 0.31221795555152454 1101 70-71 0.42844245110321566 1101 72-73 0.4883566434151234 1101 74-75 0.3391961482160859 1101 76-77 0.3441015132257128 1101 78-79 0.5073226754098634 1101 80-81 0.48641412023439656 1101 82-83 0.2592657529179121 1101 84-85 0.34121086695268943 1101 86-87 0.3012627965120096 1101 88-89 0.18531247051970112 1101 90-91 0.2897642225577677 1101 92-93 0.19130287992162565 1101 94-95 0.12798930575788248 1101 96-97 0.22093505898819998 1101 98-99 0.28994436757514563 1101 100-101 -0.16342583339370265 1102 1 0.3229094987534218 1102 2 0.36093643418433174 1102 3 0.47162458516685746 1102 4 0.26660805513141383 1102 5 0.2628675597392629 1102 6 0.3111636055811431 1102 7 0.3108160680699754 1102 8 0.4121187372299602 1102 9 0.5187108894097889 1102 10-11 0.49701230610254754 1102 12-13 0.4844989636298962 1102 14-15 0.7399919168869857 1102 16-17 0.5252391168102761 1102 18-19 0.6806815841636507 1102 20-21 0.7420550617163926 1102 22-23 0.7073816356647953 1102 24-25 0.6558318087013575 1102 26-27 0.6710189046346855 1102 28-29 0.6460806504000445 1102 30-31 0.6157812536995024 1102 32-33 0.4675646779704081 1102 34-35 0.5327177139729855 1102 36-37 0.48244535945190137 1102 38-39 0.9553889033729561 1102 40-41 0.9148049866901005 1102 42-43 0.6425050524056743 1102 44-45 0.6474426998822338 1102 46-47 0.7737774079846176 1102 48-49 0.6240888235523983 1102 50-51 0.5755143152529243 1102 52-53 0.5909272908566905 1102 54-55 0.5281869634045435 1102 56-57 0.7164294173561601 1102 58-59 0.5653630930865745 1102 60-61 0.6212811660942492 1102 62-63 0.6522093440840777 1102 64-65 0.6613796280279729 1102 66-67 0.5290553358897867 1102 68-69 0.3092464614397272 1102 70-71 0.5268578977587737 1102 72-73 0.4394863408231444 1102 74-75 0.42731135890543115 1102 76-77 0.529594795859186 1102 78-79 0.4249084597235857 1102 80-81 0.3511184501779958 1102 82-83 0.31051032861704186 1102 84-85 0.34216103278653165 1102 86-87 0.3911587120317215 1102 88-89 0.3572897468997027 1102 90-91 0.3907712796279874 1102 92-93 0.42868453153905506 1102 94-95 0.28374664408815065 1102 96-97 -0.20496159438253514 1102 98-99 -0.16529419208120544 1102 100-101 -0.2250902718963772 1103 1 0.11930655127676815 1103 2 0.17554993488407433 1103 3 0.14503696057028037 1103 4 0.22989847583230016 1103 5 0.14779601488449146 1103 6 0.033463180024916994 1103 7 0.16068879391465885 1103 8 0.05422488351847932 1103 9 0.22238256136020595 1103 10-11 0.19521591976032937 1103 12-13 0.10875102714916096 1103 14-15 0.2502898776444198 1103 16-17 0.281119075682696 1103 18-19 0.40099885917579314 1103 20-21 0.2337161947241313 1103 22-23 0.3744651118022304 1103 24-25 0.3914541132739444 1103 26-27 0.34895370742222553 1103 28-29 0.21459895074563207 1103 30-31 -0.027789973416140867 1103 32-33 0.06934740867043132 1103 34-35 -0.05583555552691877 1103 36-37 -0.1396686589127114 1103 38-39 0.0841285857766394 1103 40-41 0.1323902407385944 1103 42-43 -0.08919772584811625 1103 44-45 0.03427223462647078 1103 46-47 -0.07777415857636782 1103 48-49 -0.40331572975637187 1103 50-51 -0.49360019221573026 1103 52-53 -0.5183593491056087 1103 54-55 -0.40355133736945703 1103 56-57 -0.3868268731645017 1103 58-59 -0.2486457214965796 1103 60-61 -0.13164007030366065 1103 62-63 -0.058816739085024494 1103 64-65 -0.38138618395614543 1103 66-67 -0.4957381336786568 1103 68-69 -0.3944296731388519 1103 70-71 -0.44440634695033765 1103 72-73 -0.37607457583638393 1103 74-75 -0.3316857457414173 1103 76-77 -0.2611298100014956 1103 78-79 -0.22533796660377803 1103 80-81 -0.1261377653705118 1103 82-83 -0.3681094875940829 1103 84-85 -0.4320625591650291 1103 86-87 -0.5295908131446403 1103 88-89 -0.5589659117128107 1103 90-91 -0.45201747026490935 1103 92-93 -0.3446264529524221 1103 94-95 -0.11502800746984221 1103 96-97 -0.34431742293193324 1103 98-99 -0.4751514236808987 1103 100-101 -0.9541061017310106 1104 1 0.1211213971491425 1104 2 0.13773010619859605 1104 3 0.08645858694939079 1104 4 0.10367356315280318 1104 5 0.08806809449861674 1104 6 0.03294947902142553 1104 7 0.07053643348886851 1104 8 0.18946945024243433 1104 9 0.17515585375916487 1104 10-11 0.19846618310788955 1104 12-13 0.2557500777118875 1104 14-15 0.17953904255543307 1104 16-17 0.07416124247872347 1104 18-19 0.20939704762175637 1104 20-21 0.15251072125293064 1104 22-23 0.2692967426166675 1104 24-25 0.18521572135733066 1104 26-27 0.21086126203218925 1104 28-29 0.20092133667454704 1104 30-31 0.13268744799540144 1104 32-33 0.025051308986448362 1104 34-35 -0.13751401508227445 1104 36-37 -0.0022081610472071134 1104 38-39 0.44931715649239123 1104 40-41 0.34917163107512295 1104 42-43 0.11037771283347553 1104 44-45 0.11180600202127522 1104 46-47 0.21442468962454342 1104 48-49 0.27731687613707123 1104 50-51 0.20095994805149786 1104 52-53 0.14147876144492244 1104 54-55 0.3737055685738895 1104 56-57 0.29303648063602594 1104 58-59 0.3318654326587662 1104 60-61 0.35093524363436046 1104 62-63 0.22330170148158857 1104 64-65 0.2214007955859003 1104 66-67 0.36062006405377645 1104 68-69 0.3430309979816144 1104 70-71 0.3653020822507571 1104 72-73 0.06495982567162883 1104 74-75 0.0689529587271771 1104 76-77 0.07818269692870317 1104 78-79 0.0321388518804504 1104 80-81 -0.11033431274535488 1104 82-83 -0.1288279066770741 1104 84-85 0.0326133500770851 1104 86-87 -0.3448317068631077 1104 88-89 -0.5183926283231131 1104 90-91 -0.4104904182329747 1104 92-93 -0.2896894657871485 1104 94-95 -0.3310873576943827 1104 96-97 0.15332717567094178 1104 98-99 -0.059094325771045675 1104 100-101 -0.36333392162899614 1105 1 0.23558016142408533 1105 2 0.34151835226625593 1105 3 0.3644192904615622 1105 4 0.4218902729136289 1105 5 0.3173963642680633 1105 6 0.28972670914424725 1105 7 0.3466656351695434 1105 8 0.335174847786071 1105 9 0.3295979190468188 1105 10-11 0.32349311695835326 1105 12-13 0.33787475450568394 1105 14-15 0.2583693727644487 1105 16-17 0.3780945946657539 1105 18-19 0.504076938809007 1105 20-21 0.6325291709404937 1105 22-23 0.5716527811859464 1105 24-25 0.553694362813907 1105 26-27 0.3885825909483742 1105 28-29 0.5862134342828256 1105 30-31 0.7202965300897759 1105 32-33 0.7621971288528542 1105 34-35 0.6868604462407149 1105 36-37 0.60959425222579 1105 38-39 0.9224843079683609 1105 40-41 0.8969270313121456 1105 42-43 0.4590582908339158 1105 44-45 0.28669927663881367 1105 46-47 0.4640038482110569 1105 48-49 0.4332015857901652 1105 50-51 0.5333169293055349 1105 52-53 0.4283242688786686 1105 54-55 0.30352100436358853 1105 56-57 0.4057192941460457 1105 58-59 0.49468897553745705 1105 60-61 0.48143143249451725 1105 62-63 0.34756607069081014 1105 64-65 0.5042398927632306 1105 66-67 0.6287785293629753 1105 68-69 0.45025128994455343 1105 70-71 0.45114194704282085 1105 72-73 0.45575652896832963 1105 74-75 0.2582356429547126 1105 76-77 0.1720825164719031 1105 78-79 0.2797047051448338 1105 80-81 0.39071947415402164 1105 82-83 0.17057214180385216 1105 84-85 0.2932151869656856 1105 86-87 0.06809115458916537 1105 88-89 -0.32216101067605507 1105 90-91 -0.08764613628942541 1105 92-93 0.03447457482909755 1105 94-95 0.16230038201688402 1105 96-97 0.313566752270809 1105 98-99 0.31203056649355077 1105 100-101 0.1916700511139453 1106 1 0.17755686932007464 1106 2 0.2882889956736676 1106 3 0.2405826637101427 1106 4 0.17728375835196175 1106 5 0.2581386563556478 1106 6 0.1230436164521862 1106 7 0.03138696764872151 1106 8 0.10202055812953859 1106 9 0.17864685217647036 1106 10-11 0.25940656823054553 1106 12-13 0.4117733895029758 1106 14-15 0.35302188019721825 1106 16-17 0.47991706218329 1106 18-19 0.2907517363582386 1106 20-21 0.2886909837166627 1106 22-23 0.3647374244948054 1106 24-25 0.25174582555147396 1106 26-27 0.1677975090646413 1106 28-29 0.12333214516835511 1106 30-31 0.10826282388567421 1106 32-33 0.0761740338611574 1106 34-35 0.08476325344845037 1106 36-37 0.20886411539699168 1106 38-39 0.6371782089851408 1106 40-41 0.668458676050065 1106 42-43 0.6419916504488867 1106 44-45 0.3537462057933638 1106 46-47 0.4583914859843432 1106 48-49 0.4406811764536087 1106 50-51 0.47847412636856035 1106 52-53 0.38281350161901884 1106 54-55 0.5756513777271408 1106 56-57 0.8336956899475467 1106 58-59 0.8331410690343972 1106 60-61 0.693547268825597 1106 62-63 0.5123848095005812 1106 64-65 0.4205550424864626 1106 66-67 0.4903026181292205 1106 68-69 0.8447760850298849 1106 70-71 0.4573205964950162 1106 72-73 0.471181798765798 1106 74-75 0.6129688021721797 1106 76-77 0.6553222214604872 1106 78-79 0.7040517706353988 1106 80-81 0.715466102712309 1106 82-83 0.6495377949847558 1106 84-85 0.5007937444321371 1106 86-87 0.5009345817151036 1106 88-89 0.6812022180234969 1106 90-91 0.5330613326243174 1106 92-93 0.4249187345557708 1106 94-95 0.48842926061213277 1106 96-97 0.6811306905746584 1106 98-99 0.8354676301772521 1106 100-101 0.8553291125308569 1107 1 0.07857624579948208 1107 2 -0.03908667489049833 1107 3 0.16778546451739373 1107 4 0.08514655685956996 1107 5 0.1449509935468356 1107 6 0.2133720293412864 1107 7 0.1885565029224736 1107 8 0.22035862952157714 1107 9 0.13226938551139256 1107 10-11 0.1896829615964748 1107 12-13 0.26740930041953703 1107 14-15 0.36014403488393043 1107 16-17 0.311118467344798 1107 18-19 0.3648985884237632 1107 20-21 0.29447498374837977 1107 22-23 0.1031672437348945 1107 24-25 0.24683886199289162 1107 26-27 0.28934992706053464 1107 28-29 0.3016675204265624 1107 30-31 0.2984195564886747 1107 32-33 0.3609087620472522 1107 34-35 0.1743399207891585 1107 36-37 0.19115923896319487 1107 38-39 0.7161245757465622 1107 40-41 0.4482880266386573 1107 42-43 0.3082435882950705 1107 44-45 0.4908485756156935 1107 46-47 0.6610955496131012 1107 48-49 0.5824058298219938 1107 50-51 0.6250656977784459 1107 52-53 0.6021590547824189 1107 54-55 0.7495706443787427 1107 56-57 0.6286099092780333 1107 58-59 0.5945847693987716 1107 60-61 0.6078050588681521 1107 62-63 0.5792826299935783 1107 64-65 0.5118500986493046 1107 66-67 0.3592161951454784 1107 68-69 0.4591088117331097 1107 70-71 0.3432412270731433 1107 72-73 0.30318926864244844 1107 74-75 0.3530948067793922 1107 76-77 0.40768312276216534 1107 78-79 0.43669283196054565 1107 80-81 0.4116554347451533 1107 82-83 0.3019034541696506 1107 84-85 0.428572645599008 1107 86-87 0.3432111495367458 1107 88-89 0.1466718805706293 1107 90-91 0.4310162358212217 1107 92-93 0.39177532006087645 1107 94-95 0.4017850352946404 1107 96-97 0.4516714636513832 1107 98-99 0.5112063906693756 1107 100-101 0.2587409112882533 1108 1 0.21148850025670995 1108 2 0.1811902121730995 1108 3 0.3726897406380232 1108 4 0.03384142011282165 1108 5 0.2680103716705844 1108 6 0.2665101519851305 1108 7 0.23796863156392334 1108 8 0.2825347115950194 1108 9 0.4540276668447163 1108 10-11 0.4301446247195173 1108 12-13 0.35714019315754086 1108 14-15 0.3099885542791867 1108 16-17 0.5078227864043896 1108 18-19 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-0.9542894010620451 2316 76-77 -0.9995777753541333 2316 78-79 -0.8884013600606799 2316 80-81 -0.9548525413074991 2316 82-83 -1.0919043319985846 2316 84-85 -0.9686060948832207 2316 86-87 -1.165923679563793 2316 88-89 -1.1676621945750298 2316 90-91 -0.9355049993416635 2316 92-93 -1.0712212946789244 2316 94-95 -0.836707976107494 2316 96-97 -0.4806093754688554 2316 98-99 -0.7961621155941572 2316 100-101 -0.023748968172473184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4665.0 3 1211.0 4 1284.0 5 1340.0 6 1712.0 7 1792.0 8 2199.0 9 2667.0 10 3034.0 11 3744.0 12 4457.0 13 5684.0 14 5763.0 15 5566.0 16 6686.0 17 8136.0 18 7497.0 19 6239.0 20 6459.0 21 7023.0 22 7633.0 23 8660.0 24 9759.0 25 11001.0 26 12608.0 27 14943.0 28 17443.0 29 20942.0 30 25179.0 31 30813.0 32 39267.0 33 51315.0 34 70704.0 35 101966.0 36 156032.0 37 235495.0 38 195874.0 39 22535.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.23528642310235 19.628077330385093 15.161457372064227 23.975178874448325 2 15.123995120916609 19.521792553043923 40.66678419900074 24.687428127038732 3 19.4280825534215 23.450333532002784 28.971464981376975 28.150118933198737 4 12.974204832498796 15.70204066287977 36.081100192805856 35.24265431181558 5 14.51474141535134 36.73326332582229 33.60029292145283 15.151702337373537 6 33.65814657913164 34.701179862651934 17.09848405580134 14.542189502415084 7 29.908053379127757 31.421594933991624 19.898125290687275 18.77222639619334 8 26.67897530579275 35.20718051656925 19.213463647471986 18.900380530166004 9 26.70251537331618 16.777087362659973 19.70799197742802 36.812405286595826 10-11 25.7971809140808 25.921779914158176 26.88963596608725 21.39140320567377 12-13 26.177523715486608 23.078244000863968 27.533665414988036 23.21056686866139 14-15 22.906016392812063 24.707069948091515 25.567634438323005 26.819279220773414 16-17 23.07113770334496 28.11095823706665 26.026500519359384 22.791403540229002 18-19 22.870183803221643 26.631094254220493 28.191867395110954 22.306854547446907 20-21 23.443717853105273 25.978650234179934 27.980218740926617 22.597413171788176 22-23 23.09256856516487 25.78994228446155 28.07382220034898 23.04366695002461 24-25 23.044901930160506 26.064931188442035 28.071750363938474 22.818416517458978 26-27 22.79966174799331 26.42490894936063 27.835097672504048 22.940331630142012 28-29 22.553081826345014 26.537043077070376 28.154966674675304 22.754908421909303 30-31 22.778934955290598 26.126771778945688 28.042064595557513 23.052228670206198 32-33 22.59377291556959 26.179552030394966 28.04979806335893 23.176876990676508 34-35 23.173160879457395 26.383452890437166 27.643060637952644 22.8003255921528 36-37 23.113072917321116 26.10719587565946 27.70659847814419 23.07313272887523 38-39 22.75344575506932 26.32613847980774 27.911703970351454 23.00871179477149 40-41 22.85700956756439 26.141713351661934 27.971478080817185 23.029798999956487 42-43 22.818652524273585 26.14265331395036 27.772034673989126 23.26665948778693 44-45 22.890608313904547 26.381507745489202 27.629167765985898 23.098716174620353 46-47 22.826555172061017 26.700373503339275 27.199155713739575 23.273915610860136 48-49 23.158584893509797 26.518011045955102 27.293166577436796 23.030237483098308 50-51 22.917439796813543 26.60311106823215 27.27105064180086 23.20839849315345 52-53 23.167607344783622 26.57276190484738 27.133264553211177 23.126366197157814 54-55 22.828621566457024 26.40759613965202 27.348812833965464 23.41496945992549 56-57 22.90445387928212 26.839262599559916 27.25127177445615 23.00501174670182 58-59 23.031299724279712 26.500630550956235 27.253569384822647 23.214500339941413 60-61 23.02441066725001 26.471292273541486 27.659640277063048 22.844656782145464 62-63 22.879182915172702 26.485700566771825 27.504723098532136 23.130393419523337 64-65 22.695311711186154 26.39662541929838 27.467381670911067 23.440681198604395 66-67 22.682475855383622 26.78154058808746 27.16004559652461 23.375937960004308 68-69 22.592692665376426 26.570236731645487 27.49958707509129 23.337483527886793 70-71 22.7036939628979 26.624978344991877 27.417439440030662 23.253888252079555 72-73 22.76626280232607 26.406247615901947 27.432542927228127 23.394946654543855 74-75 22.71090825861001 26.80116864582492 27.06469738176661 23.423225713798455 76-77 22.696471918481258 26.67975357721352 27.11906994691949 23.50470455738573 78-79 22.92375497078542 26.648927961296064 27.175436424192828 23.251880643725684 80-81 22.70525592811035 26.9904261079556 27.14313464325913 23.161183320674912 82-83 22.90753247032938 26.82577741245978 27.07227732900695 23.1944127882039 84-85 22.81177439220006 26.62245623638521 27.031208286314882 23.534561085099845 86-87 22.771704601335998 26.593139454329435 27.285367902280388 23.34978804205418 88-89 22.752290696317836 26.53169282682246 27.171831390392086 23.544185086467618 90-91 22.650514922127762 26.80961008454803 27.247407213263074 23.292467780061134 92-93 22.87084595263699 26.43272108391665 27.27982269840956 23.416610265036795 94-95 22.79103208890716 26.397118290650152 27.262196594261894 23.549653026180792 96-97 22.84922336463153 26.381860720413513 27.28346596282006 23.4854499521349 98-99 22.64157971669471 26.3493308715522 27.044646411425923 23.964443000327172 100-101 22.85631209893561 25.764190180296964 27.635543554005405 23.74395416676202 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1325.0 1 773.5 2 256.0 3 311.0 4 281.0 5 250.5 6 422.0 7 846.0 8 1264.0 9 1360.5 10 1153.0 11 965.0 12 981.5 13 1066.5 14 1155.0 15 1212.5 16 1277.5 17 1320.5 18 1335.0 19 1415.0 20 1510.0 21 1604.0 22 1857.5 23 2248.5 24 2701.5 25 3340.0 26 4142.5 27 5151.0 28 6186.0 29 7378.5 30 8979.5 31 10952.5 32 13133.0 33 15193.0 34 17331.5 35 19436.5 36 22210.5 37 25191.0 38 27513.0 39 28795.5 40 29890.0 41 33337.5 42 36761.5 43 38931.5 44 40930.0 45 42921.0 46 43642.5 47 44416.5 48 47553.0 49 48276.0 50 46510.5 51 45231.0 52 43622.5 53 42513.0 54 41941.5 55 39000.0 56 34211.5 57 30282.0 58 26785.0 59 22983.0 60 19511.0 61 16283.5 62 13285.0 63 10534.5 64 8264.5 65 6579.5 66 5102.0 67 4248.0 68 3303.5 69 2272.5 70 1719.0 71 1291.5 72 1004.0 73 810.5 74 636.0 75 552.5 76 363.0 77 195.5 78 132.5 79 89.0 80 63.0 81 41.0 82 35.0 83 24.5 84 15.5 85 14.0 86 9.0 87 9.0 88 6.0 89 2.0 90 4.5 91 6.0 92 3.5 93 2.5 94 2.0 95 3.5 96 4.0 97 1.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2844558798567 2 0.17036977477598206 3 0.1693870440352711 4 0.19154315528039095 5 0.20610543807456247 6 0.1026506928251722 7 0.11524751413792178 8 0.1265042480769746 9 0.1325793108377333 10-11 0.12002715910410693 12-13 0.1100211733805044 14-15 0.12516416070327785 16-17 0.1005512226063806 18-19 0.12708495260557653 20-21 0.15245727354756863 22-23 0.15902370167868277 24-25 0.1822072132436368 26-27 0.16259726800854082 28-29 0.15960440620728472 30-31 0.1490177159550803 32-33 0.1587110146248202 34-35 0.34163294113442866 36-37 0.461347413184673 38-39 0.42940866411156675 40-41 0.41797325185602097 42-43 0.44222883331993246 44-45 0.4470531478652408 46-47 0.4481252177641982 48-49 0.4615707610802891 50-51 0.45406627178758724 52-53 0.4602306737065924 54-55 0.44441764269697054 56-57 0.4064038308631056 58-59 0.39595114934827086 60-61 0.34985214369310214 62-63 0.4578631860130614 64-65 0.457193142326213 66-67 0.4654570144640097 68-69 0.47582035682059803 70-71 0.47072802480055037 72-73 0.46250882224187684 74-75 0.4663504060464742 76-77 0.4453557038585582 78-79 0.4304360644314009 80-81 0.42780055926313065 82-83 0.44026337183851055 84-85 0.4433455727980131 86-87 0.442943546585904 88-89 0.450269357562113 90-91 0.46719912804981556 92-93 0.4731401820732045 94-95 0.47072802480055037 96-97 0.47005798111370195 98-99 0.46737780636630843 100-101 0.4493312964005253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1119330.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.729198279166276 #Duplication Level Percentage of deduplicated Percentage of total 1 82.17592149714552 51.548296733676445 2 10.593674505916937 13.290654171732246 3 3.1059009712538894 5.844920335837211 4 1.3298193007750794 3.336739943751289 5 0.7299207553710335 2.289367189587419 6 0.4405392992728975 1.6580806232312735 7 0.2795757666167666 1.2276294588707148 8 0.21127232618636896 1.0602354912196346 9 0.1541948366833233 0.8705266635538664 >10 0.8657934684399635 9.993651487659246 >50 0.07222768045518786 3.1661739231660437 >100 0.039015865339336986 4.37382257639417 >500 0.0014291510292044666 0.5713362525501762 >1k 7.145755146022333E-4 0.7685651487702275 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2817 0.2516684087802525 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1988 0.1776062465939446 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1676 0.14973242922105187 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.2334789561612753E-4 0.0 16-17 4.46695791232255E-5 0.0 0.0 2.6801747473935303E-4 0.0 18-19 8.9339158246451E-5 0.0 0.0 2.6801747473935303E-4 0.0 20-21 8.9339158246451E-5 0.0 0.0 3.5735663298580404E-4 0.0 22-23 8.9339158246451E-5 0.0 0.0 4.913653703554806E-4 0.0 24-25 8.9339158246451E-5 0.0 0.0 5.807045286019316E-4 4.46695791232255E-5 26-27 8.9339158246451E-5 0.0 0.0 7.593828450948335E-4 8.9339158246451E-5 28-29 8.9339158246451E-5 0.0 0.0 0.0011614090572038631 8.9339158246451E-5 30-31 8.9339158246451E-5 0.0 0.0 0.0029035226430096576 8.9339158246451E-5 32-33 8.9339158246451E-5 0.0 0.0 0.007727837188318012 8.9339158246451E-5 34-35 8.9339158246451E-5 0.0 0.0 0.013490212895214102 8.9339158246451E-5 36-37 8.9339158246451E-5 0.0 0.0 0.024121572726541772 8.9339158246451E-5 38-39 1.78678316492902E-4 0.0 0.0 0.041721386901092616 8.9339158246451E-5 40-41 1.78678316492902E-4 0.0 0.0 0.059767896866875725 8.9339158246451E-5 42-43 1.78678316492902E-4 0.0 0.0 0.0804052424218059 8.9339158246451E-5 44-45 1.78678316492902E-4 0.0 0.0 0.1040354497779922 8.9339158246451E-5 46-47 1.78678316492902E-4 0.0 0.0 0.13240063252124037 8.9339158246451E-5 48-49 1.78678316492902E-4 0.0 0.0 0.16559012980979693 8.9339158246451E-5 50-51 1.78678316492902E-4 0.0 0.0 0.20507803775472827 8.9339158246451E-5 52-53 1.78678316492902E-4 0.0 0.0 0.24429792822492025 8.9339158246451E-5 54-55 1.78678316492902E-4 0.0 0.0 0.2895035422976245 8.9339158246451E-5 56-57 1.78678316492902E-4 0.0 0.0 0.3435537330367273 8.9339158246451E-5 58-59 1.78678316492902E-4 0.0 0.0 0.404706386856423 8.9339158246451E-5 60-61 1.78678316492902E-4 0.0 0.0 0.47354220828531357 8.9339158246451E-5 62-63 1.78678316492902E-4 0.0 0.0 0.5438967954043936 8.9339158246451E-5 64-65 1.78678316492902E-4 0.0 0.0 0.6237213332975977 8.9339158246451E-5 66-67 1.78678316492902E-4 0.0 0.0 0.7207436591532435 8.9339158246451E-5 68-69 1.78678316492902E-4 0.0 0.0 0.827012587887397 8.9339158246451E-5 70-71 1.78678316492902E-4 0.0 0.0 0.949451904264158 8.9339158246451E-5 72-73 1.78678316492902E-4 0.0 0.0 1.077608926768692 8.9339158246451E-5 74-75 2.2334789561612753E-4 0.0 0.0 1.2195688492223027 8.9339158246451E-5 76-77 2.6801747473935303E-4 0.0 0.0 1.377341802685535 8.9339158246451E-5 78-79 2.6801747473935303E-4 0.0 0.0 1.5612911295149776 8.9339158246451E-5 80-81 4.46695791232255E-4 0.0 0.0 1.7606514611419333 8.9339158246451E-5 82-83 4.46695791232255E-4 0.0 0.0 1.9688563694352874 8.9339158246451E-5 84-85 4.46695791232255E-4 0.0 0.0 2.1801434786881435 8.9339158246451E-5 86-87 4.46695791232255E-4 0.0 0.0 2.411174541913466 8.9339158246451E-5 88-89 4.46695791232255E-4 0.0 0.0 2.6697220658786955 8.9339158246451E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3550 0.0 33.950733 1 GGTATCA 2410 0.0 33.274643 1 GTACATG 3985 0.0 26.910633 1 ATCAACG 4395 0.0 26.860537 3 TATCAAC 4490 0.0 26.5034 2 TACATGG 4060 0.0 26.391048 2 TCAACGC 4475 0.0 26.380346 4 ACATGGG 3985 0.0 26.173908 3 CAACGCA 4540 0.0 25.898228 5 AACGCAG 4680 0.0 25.110004 6 CATGGGG 2385 0.0 24.848253 4 GTACCTG 945 0.0 23.599882 1 TACCTGG 990 0.0 22.029106 2 ACGCAGA 5320 0.0 21.822046 7 CGCAGAG 5395 0.0 21.255188 8 TAGACTG 425 0.0 21.195269 5 TAAGGTG 415 0.0 20.563576 5 GAGTACT 2790 0.0 20.295277 12-13 GTACTTT 2870 0.0 19.651402 14-15 AGAGTAC 4820 0.0 19.4626 10-11 >>END_MODULE