FastQCFastQC Report
Fri 27 May 2016
SRR939343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119330
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32950.2943725264220561No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27000.24121572726541768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21180.18922033716598324No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC12510.1117632869663102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.033.6311871
GTATCAA42550.033.440311
ATCAACG52000.027.1048683
TCAACGC53050.026.6578484
TATCAAC54050.026.6036432
CAACGCA53800.026.1980135
AACGCAG55400.025.5270546
GTACATG40800.024.9604431
GTCTATA2301.3096724E-1024.828591
TACATGG40650.024.0492122
ACATGGG40600.023.611283
ACGCAGA62300.022.6236467
CGCAGAG62650.022.4972578
GAGTACT32550.020.84871312-13
CGCGGAA2200.020.59370282-83
GCAGAGT67650.020.5538889
AGTACTT34450.020.24987412-13
AGAGTAC54800.020.13430210-11
CATGGGG25550.019.874084
GTACTTT34400.019.6585114-15
Produced by FastQC (version 0.11.5)