##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939343_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1119330 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15647574888549 33.0 31.0 34.0 30.0 34.0 2 32.393078895410646 34.0 31.0 34.0 31.0 34.0 3 32.47865508831176 34.0 31.0 34.0 30.0 34.0 4 36.043784228065 37.0 35.0 37.0 35.0 37.0 5 35.9743936104634 37.0 35.0 37.0 35.0 37.0 6 36.00435349718135 37.0 35.0 37.0 35.0 37.0 7 35.947776795047034 37.0 35.0 37.0 35.0 37.0 8 35.90957358419769 37.0 35.0 37.0 35.0 37.0 9 37.60332431007835 39.0 37.0 39.0 35.0 39.0 10-11 37.502869127067086 39.0 37.0 39.0 34.5 39.0 12-13 37.40707923489945 39.0 37.0 39.0 34.0 39.0 14-15 38.64792286457077 40.0 38.0 41.0 34.0 41.0 16-17 38.64849642196671 40.0 38.0 41.0 34.0 41.0 18-19 38.75032742801497 40.0 38.0 41.0 34.0 41.0 20-21 38.72359670517184 40.0 38.0 41.0 34.0 41.0 22-23 38.64268267624382 40.0 38.0 41.0 34.0 41.0 24-25 38.56436439655866 40.0 38.0 41.0 34.0 41.0 26-27 38.386174318565565 40.0 38.0 41.0 34.0 41.0 28-29 38.200333235060256 40.0 38.0 41.0 33.0 41.0 30-31 37.99269875729231 40.0 37.5 41.0 33.0 41.0 32-33 37.96055944180894 40.0 37.0 41.0 33.0 41.0 34-35 37.852074901950274 40.0 37.0 41.0 33.0 41.0 36-37 37.69395977951096 40.0 37.0 41.0 33.0 41.0 38-39 37.55548408422896 40.0 37.0 41.0 32.0 41.0 40-41 37.35477562470406 40.0 36.5 41.0 31.5 41.0 42-43 37.16080691127728 40.0 36.0 41.0 31.0 41.0 44-45 36.90167510921712 39.0 36.0 41.0 31.0 41.0 46-47 36.58824430686214 39.0 35.5 41.0 30.0 41.0 48-49 36.27241296132508 39.0 35.0 40.5 30.0 41.0 50-51 36.01635710648334 39.0 35.0 40.0 29.0 41.0 52-53 35.73186638435493 38.5 35.0 40.0 28.0 41.0 54-55 35.441569957027866 38.0 34.5 40.0 28.0 41.0 56-57 35.07967489480314 38.0 34.0 40.0 27.0 41.0 58-59 34.71701508938383 38.0 34.0 40.0 26.0 41.0 60-61 34.321959565096975 37.0 33.0 40.0 26.0 41.0 62-63 33.92424218059017 37.0 33.0 39.0 25.5 41.0 64-65 33.552270108011044 36.0 32.5 39.0 24.5 40.0 66-67 33.08213529522125 36.0 32.0 39.0 23.0 40.0 68-69 32.55532193365674 35.0 31.5 38.0 22.0 40.0 70-71 32.01629144220203 35.0 31.0 37.5 20.5 40.0 72-73 31.471251552267873 35.0 30.5 37.0 19.0 39.0 74-75 30.879922364271486 34.0 30.0 36.5 17.5 39.0 76-77 28.993283482083033 32.5 28.0 34.5 12.5 36.5 78-79 29.96380468673224 34.0 29.0 35.0 12.5 37.0 80-81 29.944335003975592 34.0 29.5 35.0 9.5 37.0 82-83 29.532119660868556 34.0 29.0 35.0 7.0 36.5 84-85 29.001466055586825 34.0 29.0 35.0 2.0 36.0 86-87 28.297004011328205 33.0 27.5 35.0 2.0 35.5 88-89 27.615267168752737 33.0 26.0 35.0 2.0 35.0 90-91 27.014963862310488 32.0 25.5 34.5 2.0 35.0 92-93 26.507049306281438 32.0 25.0 34.0 2.0 35.0 94-95 25.8132463169932 32.0 23.5 34.0 2.0 35.0 96-97 24.876585546710977 31.5 19.0 34.0 2.0 35.0 98-99 23.62327329741899 31.0 2.0 34.0 2.0 35.0 100-101 19.253119723405966 25.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2309178616537011 1101 2 0.2608926732707104 1101 3 0.2596680410105705 1101 4 0.16466682569393498 1101 5 0.274704496202844 1101 6 0.2498346080487721 1101 7 0.15461917843263961 1101 8 0.2132828680517136 1101 9 0.2820427188804757 1101 10-11 0.3986196466138736 1101 12-13 0.3013472770897394 1101 14-15 0.3279494734737014 1101 16-17 0.40184732696683056 1101 18-19 0.45022544725235036 1101 20-21 0.420538520085195 1101 22-23 0.39741451289249596 1101 24-25 0.44185473816855136 1101 26-27 0.4013838370863638 1101 28-29 0.38834907793160056 1101 30-31 0.6074629672589182 1101 32-33 0.5571816724793166 1101 34-35 0.7085053282827047 1101 36-37 0.605376751410617 1101 38-39 0.5482392914330205 1101 40-41 0.6110735196371095 1101 42-43 0.6375702601956235 1101 44-45 0.7216225221889729 1101 46-47 0.6312081202997533 1101 48-49 0.5998392086905255 1101 50-51 0.6634701387597488 1101 52-53 0.6834376652838543 1101 54-55 0.5068357995340236 1101 56-57 0.5220036289102055 1101 58-59 0.7239209073508945 1101 60-61 0.540151754888555 1101 62-63 0.3496575070379606 1101 64-65 0.49424213020652985 1101 66-67 0.5815083227551767 1101 68-69 0.5833418815006013 1101 70-71 0.5944004199501691 1101 72-73 0.5152496545654479 1101 74-75 0.7135769000071299 1101 76-77 0.8802638713317457 1101 78-79 0.8562160298512147 1101 80-81 0.9907412999299829 1101 82-83 0.9215310238112764 1101 84-85 0.7405834703892964 1101 86-87 0.6428729264613153 1101 88-89 0.7086685311423828 1101 90-91 0.7076397574422195 1101 92-93 0.5717336441602221 1101 94-95 0.5634951075886789 1101 96-97 0.578073252664371 1101 98-99 0.3595858906964011 1101 100-101 0.03294041344308951 1102 1 0.05448745342484074 1102 2 0.16097412910193043 1102 3 0.24245090825582594 1102 4 0.1580261664496092 1102 5 0.2028717765277932 1102 6 0.2660180188199348 1102 7 0.1440490971959676 1102 8 0.18251411494689762 1102 9 0.20151285417751552 1102 10-11 0.24297554284717648 1102 12-13 0.28801710336600905 1102 14-15 0.31893088839436956 1102 16-17 0.3929218864478514 1102 18-19 0.3137458367792476 1102 20-21 0.3004596576907588 1102 22-23 0.40156446832921233 1102 24-25 0.3780867850111278 1102 26-27 0.3888768969034331 1102 28-29 0.3963837094444145 1102 30-31 0.36486795980735565 1102 32-33 0.4732410731397323 1102 34-35 0.4637460795757633 1102 36-37 0.4979523080863473 1102 38-39 0.6589991318818704 1102 40-41 0.5618137449373606 1102 42-43 0.551435284595847 1102 44-45 0.6873535426150283 1102 46-47 0.6258888353577561 1102 48-49 0.4601584388415958 1102 50-51 0.3402069195376356 1102 52-53 0.32069485019955124 1102 54-55 0.24660131266923457 1102 56-57 0.2927753224609475 1102 58-59 0.5063607834503685 1102 60-61 0.2865953677286228 1102 62-63 0.19992671308102672 1102 64-65 0.21479703435895203 1102 66-67 0.19758196905423375 1102 68-69 0.0958698248687142 1102 70-71 -0.07999541886226069 1102 72-73 0.2380217645271685 1102 74-75 -0.10497658323415493 1102 76-77 -0.16672801595968423 1102 78-79 -0.14265527724092308 1102 80-81 0.11202510592630333 1102 82-83 0.18486530685385105 1102 84-85 0.3109870602902305 1102 86-87 0.2346184920496981 1102 88-89 0.5334786806120064 1102 90-91 0.5006593031889821 1102 92-93 0.2698215324451887 1102 94-95 0.029722442412325023 1102 96-97 -0.30300899580879204 1102 98-99 -0.13159277152854543 1102 100-101 -0.10514913683305949 1103 1 0.12607871952599936 1103 2 0.22620168743740265 1103 3 0.10989121614147024 1103 4 0.05904150361009641 1103 5 0.17090329339295351 1103 6 0.06773987927176961 1103 7 0.1407351495193936 1103 8 0.21691383790693664 1103 9 0.19201102628502298 1103 10-11 0.08011951437203635 1103 12-13 0.09715362051678511 1103 14-15 0.06414338089715699 1103 16-17 0.046363108279741994 1103 18-19 0.17057669246142382 1103 20-21 0.1697930124447069 1103 22-23 0.12935441420646043 1103 24-25 0.2326858803471481 1103 26-27 0.2984533399388809 1103 28-29 0.0490033614308274 1103 30-31 0.1486937887851596 1103 32-33 0.215230655580541 1103 34-35 0.2948846923767334 1103 36-37 0.16745497495293904 1103 38-39 0.23607586052551 1103 40-41 0.15534953878535873 1103 42-43 0.2528783200398266 1103 44-45 0.31066399159112024 1103 46-47 0.09932102483023897 1103 48-49 0.06781532699742598 1103 50-51 -0.11775824697195247 1103 52-53 0.01255349912994319 1103 54-55 -0.12912743451038722 1103 56-57 0.01942105047626086 1103 58-59 -0.08255976034764245 1103 60-61 0.20870510197019598 1103 62-63 0.09509243724062344 1103 64-65 -0.0863549484759929 1103 66-67 -0.14594489953743306 1103 68-69 -0.2485442142024965 1103 70-71 -0.4303224402543151 1103 72-73 -0.33601100544398577 1103 74-75 -0.37149277007904047 1103 76-77 -0.38209846764905464 1103 78-79 -0.3307175683899253 1103 80-81 -0.10163348159833063 1103 82-83 -0.06964194759449072 1103 84-85 -0.14949993739634237 1103 86-87 -0.17593258897734998 1103 88-89 -0.1820756313578329 1103 90-91 -0.2676423811853894 1103 92-93 -0.01654754832480876 1103 94-95 -0.32486415756482856 1103 96-97 -0.31803511110882354 1103 98-99 -0.3326573761039917 1103 100-101 -0.7264594358648466 1104 1 -0.016897350496726915 1104 2 0.027813478478037723 1104 3 0.1024386533538646 1104 4 0.08951012366885891 1104 5 0.04857142002155257 1104 6 0.05205857140460779 1104 7 0.05002057669685911 1104 8 0.06467209177399269 1104 9 0.04698968127199521 1104 10-11 0.044209388658224213 1104 12-13 0.036234572171714774 1104 14-15 0.06993356219116009 1104 16-17 0.04892121799865379 1104 18-19 0.14685635371328942 1104 20-21 -0.002737757639017957 1104 22-23 0.011385101127785902 1104 24-25 -0.017161655274072984 1104 26-27 0.1189209031423033 1104 28-29 0.19220033154780225 1104 30-31 0.14114636889826215 1104 32-33 0.16359590825559422 1104 34-35 0.24900677476293254 1104 36-37 0.19196968776548573 1104 38-39 -0.02649552052454851 1104 40-41 0.0011937583063428292 1104 42-43 0.13680181757268173 1104 44-45 0.30199258004818574 1104 46-47 0.3854420884771912 1104 48-49 0.5064206937435571 1104 50-51 0.33867505679253185 1104 52-53 0.5152692708769067 1104 54-55 0.6479354258599628 1104 56-57 0.4200870426035479 1104 58-59 0.21490300715803556 1104 60-61 0.5397147973186449 1104 62-63 0.7020713209241691 1104 64-65 0.7329565709008392 1104 66-67 0.4627613362556602 1104 68-69 0.4815232339596207 1104 70-71 0.3289562299790916 1104 72-73 0.45703635900132156 1104 74-75 0.7432297447515843 1104 76-77 0.556018753202526 1104 78-79 0.754570658587955 1104 80-81 0.67523533319903 1104 82-83 0.6516795893850471 1104 84-85 0.597208651199324 1104 86-87 0.2961018922279486 1104 88-89 0.47603382767083247 1104 90-91 0.398567614240676 1104 92-93 0.44439818128654807 1104 94-95 0.2526223978490414 1104 96-97 0.12407433752454367 1104 98-99 0.3248326963858901 1104 100-101 0.14388996744501625 1105 1 0.15109917503656334 1105 2 0.052811458439258274 1105 3 -0.010525282220363863 1105 4 0.11373712216056475 1105 5 0.11622925238526705 1105 6 0.11777043307235147 1105 7 0.06555467495154943 1105 8 0.029021773954553964 1105 9 0.12515171531637748 1105 10-11 0.16395560445224078 1105 12-13 0.16279336876727513 1105 14-15 0.15430384876732717 1105 16-17 -0.017272086246116203 1105 18-19 0.10138528066869412 1105 20-21 0.2945176829987801 1105 22-23 0.1884078332975747 1105 24-25 0.13555327685261886 1105 26-27 0.19944133246787743 1105 28-29 0.149947853633563 1105 30-31 0.31642681761621105 1105 32-33 0.2793828480315028 1105 34-35 0.33648792106760794 1105 36-37 0.18903726479630478 1105 38-39 0.27457105995380005 1105 40-41 0.36423007860368983 1105 42-43 0.46797707613764317 1105 44-45 0.2731531596646519 1105 46-47 0.22153277475170086 1105 48-49 0.4027523033104643 1105 50-51 0.3455297217354385 1105 52-53 0.447385867515564 1105 54-55 0.44278533697825395 1105 56-57 0.5820537523643807 1105 58-59 0.446670473760058 1105 60-61 0.5428963584046187 1105 62-63 0.5527197533740775 1105 64-65 0.41538290681982204 1105 66-67 0.47581415353641887 1105 68-69 0.6154231881720733 1105 70-71 0.500299286432444 1105 72-73 0.47469654807694894 1105 74-75 0.38433618732861774 1105 76-77 0.5064789853723148 1105 78-79 0.48917122958558323 1105 80-81 0.6698266793528767 1105 82-83 0.7751292096177593 1105 84-85 0.8743091548623276 1105 86-87 0.8667779992092051 1105 88-89 0.9761870139453386 1105 90-91 1.2756499375546184 1105 92-93 0.8754710912197474 1105 94-95 0.8283873192022035 1105 96-97 0.9028632569384634 1105 98-99 0.9537801888444122 1105 100-101 0.5737761045921808 1106 1 -0.012141844034054827 1106 2 0.027374115513126185 1106 3 0.04781338957122472 1106 4 0.014467258989355969 1106 5 0.03350943110041982 1106 6 0.08197976633997683 1106 7 0.07132267376887569 1106 8 -0.04790951598121751 1106 9 0.07491107404435127 1106 10-11 0.06115948427943607 1106 12-13 0.0630192266164471 1106 14-15 0.10593659569155278 1106 16-17 0.0477679932558388 1106 18-19 0.05253592374758398 1106 20-21 0.0016970880481395056 1106 22-23 0.13724569200090997 1106 24-25 0.1634766987222278 1106 26-27 0.25660349019056383 1106 28-29 0.1836191524393911 1106 30-31 0.3312738913197819 1106 32-33 0.33778024286387875 1106 34-35 0.40445388634298496 1106 36-37 0.45700117681850827 1106 38-39 0.45055538952556873 1106 40-41 0.5217683923079264 1106 42-43 0.4945083923550513 1106 44-45 0.6596604117548566 1106 46-47 0.6814729840192655 1106 48-49 0.6000173700822629 1106 50-51 0.5559627117648418 1106 52-53 0.6398266106289512 1106 54-55 0.5640463629477992 1106 56-57 0.6197342333184039 1106 58-59 0.8163451563271238 1106 60-61 0.7493123639237638 1106 62-63 0.7401920344562143 1106 64-65 0.6072072633302739 1106 66-67 0.8451266422435211 1106 68-69 0.843049298982038 1106 70-71 0.8528079511608411 1106 72-73 0.7471020573658649 1106 74-75 0.5981361019132869 1106 76-77 0.7635007005778895 1106 78-79 0.8500781104476225 1106 80-81 0.8736728331990307 1106 82-83 0.9658698591028028 1106 84-85 0.934659604114124 1106 86-87 1.0439302191865387 1106 88-89 1.4453200483250122 1106 90-91 1.3621091435205486 1106 92-93 1.429656853140937 1106 94-95 1.8236656582025113 1106 96-97 1.6169695168967415 1106 98-99 1.6809734738852313 1106 100-101 1.148321103688751 1107 1 -0.08134366947525251 1107 2 0.051422043256735606 1107 3 0.06589255281816264 1107 4 0.02421457574146757 1107 5 0.08458847936518055 1107 6 -0.005066500109407457 1107 7 0.07354381225103168 1107 8 0.0927454066437079 1107 9 0.04393408237461216 1107 10-11 0.05168138441939618 1107 12-13 -0.02727231267771657 1107 14-15 0.11341083373638128 1107 16-17 0.10513854909554965 1107 18-19 0.08073351863762213 1107 20-21 0.16468630747775137 1107 22-23 0.14318868463015377 1107 24-25 0.06194584979692053 1107 26-27 0.024959205399547102 1107 28-29 0.020558564761515186 1107 30-31 0.2663605046188593 1107 32-33 0.1666665413496844 1107 34-35 0.18617846041332342 1107 36-37 0.32875243468388504 1107 38-39 0.25685601637668043 1107 40-41 0.20604200231216652 1107 42-43 0.25785960739948166 1107 44-45 0.31421985159340693 1107 46-47 0.43682897280651645 1107 48-49 0.4149412637752903 1107 50-51 0.2933319195376285 1107 52-53 0.4934891894809539 1107 54-55 0.4625845033291469 1107 56-57 0.2176796207488607 1107 58-59 0.24891564238453867 1107 60-61 0.354567445937775 1107 62-63 0.424119904946636 1107 64-65 0.2573842962350099 1107 66-67 0.33707788980015607 1107 68-69 0.5849507870100155 1107 70-71 0.5712384047853547 1107 72-73 0.5866234143141611 1107 74-75 0.33259626058869074 1107 76-77 0.3199026306925141 1107 78-79 0.38253361432728283 1107 80-81 0.6634164229426212 1107 82-83 0.8259060190152567 1107 84-85 0.7728344801117899 1107 86-87 0.7709399292332044 1107 88-89 0.911863406518286 1107 90-91 0.9696943988404598 1107 92-93 0.9443892420344504 1107 94-95 0.9300356708505539 1107 96-97 0.8900585329268438 1107 98-99 0.8994035310194803 1107 100-101 0.7886144271891276 1108 1 0.07330816708889643 1108 2 0.06701396611435229 1108 3 0.06419807970926428 1108 4 0.11187931938892604 1108 5 0.11141378934525648 1108 6 0.04274580582840315 1108 7 0.08782678926021248 1108 8 0.12042949591057805 1108 9 0.14675674214333867 1108 10-11 0.07239366334722064 1108 12-13 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 13.0 5 32.0 6 60.0 7 147.0 8 354.0 9 718.0 10 1014.0 11 1571.0 12 2206.0 13 3128.0 14 4140.0 15 5358.0 16 6917.0 17 9473.0 18 9083.0 19 7462.0 20 7543.0 21 8029.0 22 8813.0 23 9914.0 24 11372.0 25 13319.0 26 15959.0 27 19120.0 28 22651.0 29 27468.0 30 32782.0 31 40220.0 32 49746.0 33 63214.0 34 84234.0 35 117933.0 36 172871.0 37 231873.0 38 126118.0 39 4469.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.69108068542022 20.353855443923013 15.640202766265112 22.314861104391657 2 16.59349789606282 20.781002921390474 39.505418419947645 23.120080762599056 3 19.874478482663736 24.191435948290497 29.479778081530917 26.454307487514857 4 13.923954508500621 16.98078314706119 35.87378163722941 33.22148070720878 5 14.482592265015679 37.06628072150304 33.566597875514816 14.884529137966462 6 33.177525841351525 35.77765270295623 16.697935372052925 14.34688608363932 7 29.719921738897376 31.923829433679074 19.847766074348048 18.5084827530755 8 26.454218148356606 36.36952462633897 18.726112942563855 18.45014428274057 9 26.404366898055088 17.345286912706708 19.542047474828692 36.708298714409516 10-11 25.835142451287822 26.54579078555922 26.560040381299526 21.05902638185343 12-13 26.361707449992405 23.455281284339737 27.485951417365744 22.69705984830211 14-15 22.839102486788796 25.202824942040447 25.534679692490496 26.423392878680264 16-17 22.851508058464066 28.58159418218204 26.057159704106063 22.50973805524783 18-19 22.75687241474811 26.916994988073224 28.42798817149545 21.89814442568322 20-21 23.401499111075374 26.162838483735808 28.387070837018573 22.048591568170245 22-23 23.12722789525877 25.929439932816955 28.37304458917388 22.570287582750396 24-25 23.022209714740068 26.18142102865107 28.448714856208625 22.347654400400238 26-27 22.82068096154035 26.468491390824322 28.32496075773968 22.38586688989564 28-29 22.521329724031343 26.677655383130983 28.562845630868466 22.238169261969215 30-31 22.802435385453798 26.242082317100408 28.433169842673745 22.52231245477205 32-33 22.68808126289834 26.386633075143166 28.335566812289493 22.589718849668998 34-35 23.253311391400835 26.43069261818357 28.01813246995045 22.297863520465143 36-37 23.196597729288072 26.171708349992517 28.176260495285916 22.455433425433494 38-39 22.86062974433472 26.383515345143444 28.33443000530244 22.4214249052194 40-41 22.878978137470245 26.371104169207342 28.337038808994457 22.41287888432796 42-43 22.993627870306057 26.36989621000109 27.87343293182737 22.763042987865482 44-45 23.056141107920165 26.459477170605016 27.81110922007105 22.673272501403762 46-47 23.110786114525858 26.76829311872838 27.49783798850739 22.62308277823837 48-49 23.298272039723923 26.640910083795998 27.431966840744714 22.62885103573536 50-51 23.06062555278604 26.737155262523117 27.428327660296787 22.773891524394056 52-53 23.411470885025366 26.70580914735116 27.21749940477759 22.665220562845885 54-55 22.95386525868153 26.577997552107064 27.403491374304274 23.06464581490713 56-57 23.033988432283113 27.070315780445664 27.249486742867557 22.646209044403665 58-59 23.233383140915254 26.530779075455136 27.413601713559498 22.822236070070108 60-61 23.245390293954472 26.65441521348172 27.718853271129067 22.38134122143474 62-63 23.037888283658695 26.738180515439325 27.482518357176744 22.741412843725236 64-65 22.832305261592108 26.451216063916895 27.529630488279356 23.186848186211645 66-67 22.80609045086061 26.92008405058044 27.313401100305008 22.96042439825395 68-69 22.74658652226857 26.702093158928736 27.53845439122033 23.012865927582364 70-71 22.818293086042544 26.738450680317687 27.457943591255486 22.985312642384283 72-73 22.943635925062313 26.650764296498796 27.332243395602728 23.07335638283616 74-75 22.83878235924485 26.874728911128415 27.10598363215088 23.180505097475855 76-77 22.89389493746619 26.853085849195175 27.16378448674269 23.089234726595947 78-79 22.992514715282674 26.768703942851502 27.207493414584356 23.031287927281465 80-81 22.804173575573916 27.132172160439122 27.171928156894136 22.89172610709283 82-83 22.99080700061644 26.91458283080057 27.14405045875658 22.950559709826415 84-85 22.938900949675254 26.739969446007876 27.037111486335576 23.284018117981294 86-87 22.95160622475252 26.603743043389215 27.317625679563506 23.12702505229475 88-89 22.79300576218103 26.720551031817298 27.11121193196923 23.375231274032437 90-91 22.790368525717973 26.916396438361073 27.210930361717757 23.0823046742032 92-93 22.91455602905309 26.574826011989316 27.300393985687865 23.210223973269724 94-95 22.775142272609507 26.541413166805146 27.268633914931257 23.414810645654097 96-97 22.79582428774356 26.486826941116558 27.275602369274477 23.4417464018654 98-99 22.63241873695646 26.43179657794677 27.119753995254296 23.816030689842478 100-101 22.85413467199487 25.803719556483497 27.791521155122677 23.55062461639896 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 264.0 1 183.0 2 161.5 3 232.0 4 187.5 5 146.5 6 303.0 7 800.5 8 1291.0 9 1388.5 10 1131.0 11 892.0 12 888.5 13 931.5 14 1015.5 15 1109.5 16 1197.0 17 1276.5 18 1291.0 19 1331.5 20 1447.0 21 1648.0 22 1934.0 23 2316.5 24 2824.5 25 3464.0 26 4242.0 27 5340.5 28 6496.5 29 7792.5 30 9515.0 31 11531.5 32 13810.0 33 15852.0 34 17915.5 35 20135.0 36 22855.5 37 25701.5 38 28072.0 39 29500.0 40 30696.5 41 34136.0 42 37741.5 43 39809.0 44 41374.5 45 43123.5 46 44007.5 47 44823.0 48 48168.0 49 49022.5 50 46885.5 51 45256.5 52 43344.0 53 42129.0 54 41515.0 55 38432.0 56 33414.0 57 29298.5 58 25967.0 59 22449.5 60 18733.5 61 15250.5 62 12512.5 63 9975.5 64 7911.0 65 6415.0 66 4788.5 67 3851.5 68 2853.5 69 1778.5 70 1262.0 71 976.0 72 790.5 73 649.5 74 501.5 75 411.5 76 308.5 77 192.5 78 105.5 79 62.5 80 48.0 81 34.5 82 17.5 83 10.5 84 7.0 85 3.0 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2830264533247568 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001340087373696765 16-17 8.933915824645099E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.933915824645101E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001072069898957412 36-37 0.006923784764099952 38-39 0.0030822009595025597 40-41 0.0036182359089812653 42-43 2.2334789561612747E-4 44-45 0.0014740961110664417 46-47 1.7867831649290202E-4 48-49 4.020262121090295E-4 50-51 0.0 52-53 4.4669579123225505E-5 54-55 0.0 56-57 7.147132659716081E-4 58-59 0.0020101310605451475 60-61 6.253741077251571E-4 62-63 0.0011614090572038631 64-65 1.340087373696765E-4 66-67 3.5735663298580404E-4 68-69 0.001518765690189667 70-71 0.0 72-73 0.0 74-75 1.340087373696765E-4 76-77 0.2514003913055131 78-79 4.4669579123225505E-5 80-81 1.7867831649290202E-4 82-83 0.0 84-85 0.0 86-87 0.1026506928251722 88-89 0.36097486889478525 90-91 0.2671240831568885 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.7867831649290202E-4 100-101 2.68017474739353E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1119330.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.25844904850308 #Duplication Level Percentage of deduplicated Percentage of total 1 81.21067799207218 49.74840179971744 2 11.164046116719781 13.677843004324346 3 3.295866215821783 6.0569895795780395 4 1.3916412510801999 3.409991386923661 5 0.7372652928688833 2.25818641892191 6 0.46751870625833336 1.7183682507928912 7 0.30960207021955666 1.327601985069905 8 0.21104844740476972 1.0342800449688614 9 0.16573145455059426 0.9137206677889695 >10 0.9256619400048736 10.451380429916595 >50 0.07562711886685033 3.2317670951742326 >100 0.04326698456297536 4.667061278120529 >500 0.0014617211208643173 0.6659295716071549 >1k 5.846884483457268E-4 0.8384784870955222 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3295 0.2943725264220561 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2700 0.24121572726541768 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2118 0.18922033716598324 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1251 0.1117632869663102 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.2334789561612753E-4 0.0 16-17 4.46695791232255E-5 0.0 0.0 2.6801747473935303E-4 0.0 18-19 1.78678316492902E-4 0.0 0.0 2.6801747473935303E-4 0.0 20-21 1.78678316492902E-4 0.0 0.0 3.5735663298580404E-4 0.0 22-23 1.78678316492902E-4 0.0 0.0 4.913653703554806E-4 0.0 24-25 1.78678316492902E-4 0.0 0.0 5.807045286019316E-4 0.0 26-27 1.78678316492902E-4 0.0 0.0 7.593828450948335E-4 0.0 28-29 3.57356632985804E-4 0.0 0.0 0.0011614090572038631 0.0 30-31 3.57356632985804E-4 0.0 0.0 0.0029481922221328834 0.0 32-33 3.57356632985804E-4 0.0 0.0 0.007906515504810913 0.0 34-35 4.020262121090295E-4 0.0 0.0 0.01340087373696765 0.0 36-37 4.46695791232255E-4 0.0 0.0 0.02403223356829532 0.0 38-39 4.46695791232255E-4 0.0 0.0 0.041721386901092616 0.0 40-41 4.46695791232255E-4 0.0 0.0 0.060214592658107975 0.0 42-43 4.46695791232255E-4 0.0 0.0 0.08098594695040784 0.0 44-45 4.46695791232255E-4 0.0 0.0 0.10466082388571735 0.0 46-47 4.46695791232255E-4 0.0 0.0 0.13342803284107457 0.0 48-49 4.46695791232255E-4 0.0 0.0 0.16608149518015242 0.0 50-51 4.46695791232255E-4 0.0 0.0 0.2056587422833302 0.0 52-53 4.46695791232255E-4 0.0 0.0 0.24501264149089189 0.0 54-55 4.46695791232255E-4 0.0 0.0 0.29053094261745865 0.0 56-57 4.46695791232255E-4 0.0 0.0 0.3447598116730544 0.0 58-59 4.46695791232255E-4 0.0 0.0 0.40622515254661273 0.0 60-61 4.46695791232255E-4 0.0 0.0 0.4757756872414748 0.0 62-63 4.46695791232255E-4 0.0 0.0 0.5463536222561711 0.0 64-65 4.46695791232255E-4 0.0 0.0 0.6260441514120054 0.0 66-67 4.46695791232255E-4 0.0 0.0 0.7223070944225563 0.0 68-69 4.46695791232255E-4 0.0 0.0 0.8285313535775867 0.0 70-71 4.46695791232255E-4 0.0 0.0 0.9501666175301297 0.0 72-73 4.46695791232255E-4 0.0 0.0 1.0771622309774598 0.0 74-75 4.46695791232255E-4 0.0 0.0 1.218854135956331 0.0 76-77 4.46695791232255E-4 0.0 0.0 1.376537750261317 0.0 78-79 4.46695791232255E-4 0.0 0.0 1.559146989717063 0.0 80-81 5.360349494787061E-4 0.0 0.0 1.7576139297615536 0.0 82-83 5.360349494787061E-4 0.0 0.0 1.9647467681559503 0.0 84-85 5.807045286019316E-4 0.0 0.0 2.1750958162472194 8.9339158246451E-5 86-87 6.25374107725157E-4 0.0 0.0 2.4042954267284893 8.9339158246451E-5 88-89 6.25374107725157E-4 0.0 0.0 2.6607881500540502 8.9339158246451E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2830 0.0 33.631187 1 GTATCAA 4255 0.0 33.44031 1 ATCAACG 5200 0.0 27.104868 3 TCAACGC 5305 0.0 26.657848 4 TATCAAC 5405 0.0 26.603643 2 CAACGCA 5380 0.0 26.198013 5 AACGCAG 5540 0.0 25.527054 6 GTACATG 4080 0.0 24.960443 1 GTCTATA 230 1.3096724E-10 24.82859 1 TACATGG 4065 0.0 24.049212 2 ACATGGG 4060 0.0 23.61128 3 ACGCAGA 6230 0.0 22.623646 7 CGCAGAG 6265 0.0 22.497257 8 GAGTACT 3255 0.0 20.848713 12-13 CGCGGAA 220 0.0 20.593702 82-83 GCAGAGT 6765 0.0 20.553888 9 AGTACTT 3445 0.0 20.249874 12-13 AGAGTAC 5480 0.0 20.134302 10-11 CATGGGG 2555 0.0 19.87408 4 GTACTTT 3440 0.0 19.65851 14-15 >>END_MODULE