FastQCFastQC Report
Fri 27 May 2016
SRR939342_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939342_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138829
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28020.2460422065121278No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20810.18273156022545967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16830.1477833809992545No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC11820.10379082373209675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37000.036.832151
ATACCGT958.858915E-734.9190146
GGTATCA23150.031.9978281
ATCAACG45800.029.4040783
TCAACGC46200.029.1494944
TATCAAC46550.028.9303262
CAACGCA46550.028.7265915
AACGCAG48150.028.0502076
TACCGTG1405.9247213E-727.080057
GTACATG42100.025.6029871
TACATGG42650.024.3489232
ACGCAGA55500.024.0792877
CGCAGAG56200.023.6950428
ACATGGG42500.023.4306893
ACCGTGC1954.316571E-721.8723478
TAAGGCG2406.044502E-921.7338225
GCAGAGT60450.021.5587659
GAGTACT27800.020.71185312-13
AGAGTAC52150.019.94654710-11
AGTACTT29300.019.73239112-13
Produced by FastQC (version 0.11.5)