##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939342_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1138829 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.824205389922454 33.0 31.0 34.0 30.0 34.0 2 31.935597003588775 33.0 31.0 34.0 30.0 34.0 3 31.987243914582436 34.0 31.0 34.0 30.0 34.0 4 35.48813474191472 37.0 35.0 37.0 33.0 37.0 5 35.521720117770094 37.0 35.0 37.0 33.0 37.0 6 35.60452974063709 37.0 35.0 37.0 33.0 37.0 7 35.51761765813831 37.0 35.0 37.0 33.0 37.0 8 35.49576099660265 37.0 35.0 37.0 33.0 37.0 9 37.124758853172864 39.0 37.0 39.0 33.0 39.0 10-11 37.02397462656817 39.0 37.0 39.0 33.0 39.0 12-13 36.907748661124714 39.0 37.0 39.0 33.0 39.0 14-15 38.14764727628116 40.0 38.0 41.0 33.0 41.0 16-17 38.07090397241377 40.0 38.0 41.0 33.0 41.0 18-19 37.97882825252957 40.0 38.0 41.0 33.0 41.0 20-21 37.858141564712525 40.0 38.0 41.0 32.5 41.0 22-23 37.77600544067634 40.0 38.0 41.0 32.0 41.0 24-25 37.80964218508661 40.0 38.0 41.0 32.0 41.0 26-27 37.73272150603822 40.0 38.0 41.0 32.0 41.0 28-29 37.588315717285035 40.0 37.5 41.0 32.0 41.0 30-31 37.42278120771424 40.0 37.0 41.0 31.5 41.0 32-33 37.240846957708314 40.0 37.0 41.0 31.0 41.0 34-35 36.939104114840774 40.0 36.5 41.0 30.5 41.0 36-37 36.70177085409662 40.0 36.0 41.0 30.0 41.0 38-39 36.38391452974942 39.0 35.5 41.0 30.0 41.0 40-41 36.26360410562077 39.0 35.0 41.0 30.0 41.0 42-43 36.1112836079868 39.0 35.0 41.0 29.5 41.0 44-45 36.1079578233431 39.0 35.0 41.0 29.0 41.0 46-47 35.94757948735061 39.0 35.0 41.0 28.0 41.0 48-49 35.773803617575595 39.0 35.0 41.0 27.5 41.0 50-51 34.65859580323297 38.0 34.0 40.0 25.5 40.5 52-53 34.782998149853924 38.0 34.5 39.5 26.0 40.5 54-55 35.27612266635289 39.0 35.0 40.0 26.0 41.0 56-57 35.166272987428314 39.0 35.0 40.5 26.0 41.0 58-59 34.948290744264504 38.0 34.5 40.0 26.0 41.0 60-61 34.645916990171486 38.0 34.0 40.0 26.0 41.0 62-63 34.27337422914239 37.5 34.0 40.0 24.5 41.0 64-65 33.887817222778835 37.0 34.0 40.0 23.0 41.0 66-67 33.51674922222739 36.5 33.0 39.0 22.5 41.0 68-69 33.094401793421135 36.0 33.0 39.0 22.0 41.0 70-71 32.668869953259005 35.5 32.5 39.0 20.5 40.0 72-73 32.21892268286108 35.0 32.0 38.0 20.0 40.0 74-75 31.74445197654784 35.0 31.5 37.0 18.0 39.0 76-77 31.279037063509975 35.0 31.0 36.5 17.0 39.0 78-79 30.8483341221553 35.0 31.0 36.0 12.0 38.5 80-81 30.371715156533597 34.5 30.5 36.0 7.5 37.0 82-83 29.928628002974985 34.0 30.0 35.0 4.5 37.0 84-85 29.5021140136052 34.0 29.5 35.0 2.0 36.5 86-87 29.15718294844968 34.0 29.0 35.0 2.0 36.0 88-89 28.73412118939718 34.0 29.0 35.0 2.0 36.0 90-91 28.332274643515397 34.0 29.0 35.0 2.0 35.0 92-93 27.901491356472306 34.0 27.5 35.0 2.0 35.0 94-95 27.4043877526828 34.0 26.5 35.0 2.0 35.0 96-97 26.803243507146377 33.0 25.5 35.0 2.0 35.0 98-99 26.024548461621542 33.0 24.5 35.0 2.0 35.0 100-101 23.433529968063688 30.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.393003254516632 1101 2 0.15850030703169793 1101 3 0.2727096560787672 1101 4 0.39219007405045403 1101 5 0.26684831968781 1101 6 0.32492377812386053 1101 7 0.34738303412685667 1101 8 0.247852331904717 1101 9 0.2683094426236394 1101 10-11 0.2925697009754842 1101 12-13 0.37274542814529354 1101 14-15 0.4212511198632569 1101 16-17 0.5017913581021602 1101 18-19 0.5338135815961351 1101 20-21 0.6676710956789051 1101 22-23 0.618041158134524 1101 24-25 0.38125192527981966 1101 26-27 0.40706664214323496 1101 28-29 0.5594987918970702 1101 30-31 0.5613762903857449 1101 32-33 0.591611858226031 1101 34-35 0.4000564439409402 1101 36-37 0.2835467674805301 1101 38-39 0.4235447422665217 1101 40-41 0.382514708269035 1101 42-43 0.350890049638501 1101 44-45 0.4797934452942556 1101 46-47 0.28004545004143466 1101 48-49 0.2576507315272494 1101 50-51 0.35861307405060217 1101 52-53 0.6003780403032337 1101 54-55 0.4396344021228771 1101 56-57 0.23740394083818472 1101 58-59 0.14680362902417698 1101 60-61 0.239301641549595 1101 62-63 0.33203153190474666 1101 64-65 0.2995898580322418 1101 66-67 0.4886690615744911 1101 68-69 0.2516629191452253 1101 70-71 0.09582828254113451 1101 72-73 0.17621008939718763 1101 74-75 0.2336979904704357 1101 76-77 0.07664965814920777 1101 78-79 0.17057856099940238 1101 80-81 0.14052465541543668 1101 82-83 0.177328419933378 1101 84-85 -0.1338295670029801 1101 86-87 -0.0633313266141613 1101 88-89 -0.00819216267776568 1101 90-91 0.11688035937022079 1101 92-93 0.19502242319223484 1101 94-95 0.19320759126474485 1101 96-97 0.09791720976540219 1101 98-99 0.2537941041055234 1101 100-101 0.06274994092431285 1102 1 0.09290212615610827 1102 2 0.19523273733831203 1102 3 0.3146887997136574 1102 4 0.22710889951160596 1102 5 0.21135730343839754 1102 6 0.2357332762154769 1102 7 0.24509930926848966 1102 8 0.3825841242689805 1102 9 0.20158647271205155 1102 10-11 0.1880264459471519 1102 12-13 0.28591413489841955 1102 14-15 0.415815017401016 1102 16-17 0.5153041368847795 1102 18-19 0.37592375713902015 1102 20-21 0.4446087549379456 1102 22-23 0.22614293531140817 1102 24-25 0.26415461732402434 1102 26-27 0.26685978208821126 1102 28-29 0.23105589679872196 1102 30-31 0.31377718278009326 1102 32-33 0.28787518342801377 1102 34-35 0.30488432637106655 1102 36-37 0.28212363047153133 1102 38-39 0.23439257126897672 1102 40-41 0.2841098359843741 1102 42-43 0.09616166446631524 1102 44-45 0.315515588780535 1102 46-47 0.17002440881803693 1102 48-49 0.27503375428656085 1102 50-51 0.3224084866651822 1102 52-53 0.3787066676035309 1102 54-55 0.20651586083604911 1102 56-57 0.1397004426668289 1102 58-59 0.029520556606847492 1102 60-61 0.1535210502205402 1102 62-63 0.20427995245373864 1102 64-65 0.23812674088645025 1102 66-67 0.0318044757114464 1102 68-69 0.22061624061379348 1102 70-71 0.005610186001611339 1102 72-73 -0.06644043418674528 1102 74-75 -0.11949190249299235 1102 76-77 -0.026154207201464885 1102 78-79 -0.13988324039030076 1102 80-81 -0.16138065364225795 1102 82-83 -0.38816216135533566 1102 84-85 -0.2780308160650975 1102 86-87 -0.43769882127683246 1102 88-89 -0.24744591511989 1102 90-91 -0.3521214420919705 1102 92-93 -0.32573970043084444 1102 94-95 -0.12702496771773752 1102 96-97 -0.16753612801419493 1102 98-99 -0.3483727250672892 1102 100-101 -0.5134098636333526 1103 1 0.428040438172534 1103 2 0.39372185908657187 1103 3 0.36568631622544956 1103 4 0.34821120274085615 1103 5 0.331404213771215 1103 6 0.2558251616954905 1103 7 0.41202967483938835 1103 8 0.3307471314040811 1103 9 0.38351183905343333 1103 10-11 0.4297817488932978 1103 12-13 0.335623767464007 1103 14-15 0.6178815447821009 1103 16-17 0.4135366673549967 1103 18-19 0.4947934295550951 1103 20-21 0.5850638102520094 1103 22-23 0.61699712265888 1103 24-25 0.6590601515965275 1103 26-27 0.5658880680513079 1103 28-29 0.5754387517708892 1103 30-31 0.4874865214841648 1103 32-33 0.5599210688291834 1103 34-35 0.4500241435318557 1103 36-37 0.5172993023213905 1103 38-39 0.6587451800067043 1103 40-41 0.6213575899147727 1103 42-43 0.5361897246577527 1103 44-45 0.6581334827968703 1103 46-47 0.6853531395154562 1103 48-49 0.5509096945538374 1103 50-51 0.5335905446275788 1103 52-53 0.6165956309532774 1103 54-55 0.725249084747773 1103 56-57 0.6826100479964765 1103 58-59 0.587400076967505 1103 60-61 0.7760926610475423 1103 62-63 0.6693749378144176 1103 64-65 0.7103940079384827 1103 66-67 0.5306236541176403 1103 68-69 0.5371064410488557 1103 70-71 0.5344354924955894 1103 72-73 0.6483369477938083 1103 74-75 0.7942794517427352 1103 76-77 0.775425656833832 1103 78-79 0.6786621988430817 1103 80-81 0.3441839009246017 1103 82-83 0.7451094132765306 1103 84-85 0.5327125102322867 1103 86-87 0.38828185195274045 1103 88-89 0.44253376543113987 1103 90-91 0.34773843613468003 1103 92-93 0.4892099668247383 1103 94-95 0.5546226636509068 1103 96-97 0.40326344409476533 1103 98-99 0.1501955959441652 1103 100-101 0.011872978079214391 1104 1 0.13924264776093054 1104 2 0.1299155964590888 1104 3 0.32093490785429424 1104 4 0.0828648507950902 1104 5 0.2578308874973416 1104 6 0.25494819034236116 1104 7 0.2648856092277825 1104 8 0.19004971066564735 1104 9 0.2971374904224007 1104 10-11 0.14218903956919604 1104 12-13 0.31792783076461006 1104 14-15 0.09855325137525028 1104 16-17 0.4197479010389671 1104 18-19 0.24107061286453302 1104 20-21 0.31051658875077237 1104 22-23 0.21344068907803404 1104 24-25 0.27597586835461385 1104 26-27 0.3166747122173774 1104 28-29 0.1709764593832972 1104 30-31 0.32979120564944253 1104 32-33 0.4990948704094649 1104 34-35 0.283457792500144 1104 36-37 0.3901725174432471 1104 38-39 0.4198925034332248 1104 40-41 0.3219887161029291 1104 42-43 0.24236077460027872 1104 44-45 0.5335923405955754 1104 46-47 0.4846340574983685 1104 48-49 0.5590003373471077 1104 50-51 0.5596384046708849 1104 52-53 0.6954605351116001 1104 54-55 0.6904453081659341 1104 56-57 0.750365354880401 1104 58-59 0.6705002267511091 1104 60-61 0.700231189753481 1104 62-63 0.786022044369453 1104 64-65 0.8730864220931664 1104 66-67 0.8104598665671787 1104 68-69 0.8014986447131562 1104 70-71 0.7359578181856818 1104 72-73 0.5082297796524529 1104 74-75 0.36286732090929874 1104 76-77 0.4866571850318664 1104 78-79 0.48983569344026634 1104 80-81 0.3097552300837698 1104 82-83 0.5292869357332926 1104 84-85 0.2700169055230255 1104 86-87 0.35761329279856824 1104 88-89 0.18376340516578793 1104 90-91 0.1901785032915413 1104 92-93 0.12913734290403056 1104 94-95 0.16837541981579207 1104 96-97 0.2654060480874456 1104 98-99 0.12628626978067103 1104 100-101 -0.03767245412451814 1105 1 0.14185825259950136 1105 2 0.26812148903951893 1105 3 0.28306392072722275 1105 4 0.17807911263579257 1105 5 0.20713761295252198 1105 6 -0.028395496250958274 1105 7 -0.026567380782445582 1105 8 0.11280454434390208 1105 9 0.09772526299728668 1105 10-11 0.2220645810053128 1105 12-13 0.25523982625418995 1105 14-15 0.21778946775388874 1105 16-17 0.23258487433093222 1105 18-19 0.3879023465066638 1105 20-21 0.3555076928246734 1105 22-23 0.2389844040302762 1105 24-25 0.009012417613057266 1105 26-27 0.22920176292247163 1105 28-29 0.14336490582036276 1105 30-31 0.0976311667572034 1105 32-33 0.11695491909229361 1105 34-35 0.20717902422298096 1105 36-37 0.2803487648141356 1105 38-39 0.42075171147634904 1105 40-41 0.40318894027351604 1105 42-43 -0.02798422528156408 1105 44-45 -0.08269446271161485 1105 46-47 0.06541607186014176 1105 48-49 0.004435140505755442 1105 50-51 0.23590307788486342 1105 52-53 0.07825628667387008 1105 54-55 0.19503886393680148 1105 56-57 0.20651747015268995 1105 58-59 0.1786724093421057 1105 60-61 0.2245595220121004 1105 62-63 0.2978023943321517 1105 64-65 0.12321471248860405 1105 66-67 0.06946424869700962 1105 68-69 0.06158336701509626 1105 70-71 0.06113261384946611 1105 72-73 0.15840803578085172 1105 74-75 0.22433395021420566 1105 76-77 0.08272643483689635 1105 78-79 -0.14744220381263418 1105 80-81 -0.032185516633852274 1105 82-83 -0.015587694165798638 1105 84-85 0.07327683199237356 1105 86-87 0.06913756837983698 1105 88-89 0.2952139981712598 1105 90-91 0.07332367737823375 1105 92-93 0.17796787466772201 1105 94-95 0.42615260303135116 1105 96-97 0.5175076473285039 1105 98-99 0.7275694269397839 1105 100-101 0.6264940971933299 1106 1 0.13885763540471885 1106 2 0.16923243965080204 1106 3 0.33236399557851115 1106 4 0.20924088332822777 1106 5 0.329647494634969 1106 6 0.29226345766954154 1106 7 0.27704320870423516 1106 8 0.14326704647782407 1106 9 0.28004066266374394 1106 10-11 0.3063707621454128 1106 12-13 0.33211997227984824 1106 14-15 0.28337790388735584 1106 16-17 0.3153021071972191 1106 18-19 0.31672961075794603 1106 20-21 0.23124799827041898 1106 22-23 0.38278914723785107 1106 24-25 0.2925871572750438 1106 26-27 0.27633673160311645 1106 28-29 0.22360186922202985 1106 30-31 0.26034734907916146 1106 32-33 0.29110069524755744 1106 34-35 0.4102640132579296 1106 36-37 0.44686660436975245 1106 38-39 0.40779554543782837 1106 40-41 0.47677792794275575 1106 42-43 0.16062623288707556 1106 44-45 0.24758025462630684 1106 46-47 0.10695525059712452 1106 48-49 0.28316096157179516 1106 50-51 0.38113918771926336 1106 52-53 0.1853806185933493 1106 54-55 0.2972699574221167 1106 56-57 0.3124911277540434 1106 58-59 0.38252889185710615 1106 60-61 0.3544888729070337 1106 62-63 0.29478372090698457 1106 64-65 0.30183186787724026 1106 66-67 0.1856987695831407 1106 68-69 0.4802860104472231 1106 70-71 0.3162589418031416 1106 72-73 0.3704193685276209 1106 74-75 0.3467814558280544 1106 76-77 0.3477869724529512 1106 78-79 0.2531894359557221 1106 80-81 0.2071531000619231 1106 82-83 0.05374987198566572 1106 84-85 0.15354334195284736 1106 86-87 0.10582782852087647 1106 88-89 0.18720491096295078 1106 90-91 0.22742539633136616 1106 92-93 0.27683394339958056 1106 94-95 0.13438525920133415 1106 96-97 0.20697167275167416 1106 98-99 0.16750619730392557 1106 100-101 0.285612165242334 1107 1 0.01053546477997358 1107 2 -0.03960816720441329 1107 3 -0.1345531592548248 1107 4 -0.014423951233325738 1107 5 -0.12515322356745884 1107 6 -0.20408578763662888 1107 7 -0.10631671275852028 1107 8 -0.10964742155506713 1107 9 0.12124171413276486 1107 10-11 0.007810557793568762 1107 12-13 0.04293127621769344 1107 14-15 0.12645519461079147 1107 16-17 0.18325490734705596 1107 18-19 0.0508114483744464 1107 20-21 0.14783931396301853 1107 22-23 0.061272461302863235 1107 24-25 -0.17829964833881462 1107 26-27 -0.05281650644352709 1107 28-29 -0.03552905417186025 1107 30-31 0.07449309508610469 1107 32-33 -0.16311955134388256 1107 34-35 -0.053487593332484096 1107 36-37 0.005461943804363045 1107 38-39 0.10507273798970118 1107 40-41 -0.0015331015022539418 1107 42-43 -0.21902399865570032 1107 44-45 -0.11427073128736964 1107 46-47 -0.28811340490467785 1107 48-49 -0.11781574851856647 1107 50-51 -0.15458201798243465 1107 52-53 -0.0858013475503796 1107 54-55 0.011244387607284523 1107 56-57 -0.08127610878128166 1107 58-59 -0.14470725594275535 1107 60-61 -0.14765620785845357 1107 62-63 -0.2722990984250586 1107 64-65 -0.43783832090924335 1107 66-67 -0.3640054240214923 1107 68-69 -0.243811553994 1107 70-71 -0.2114010552258776 1107 72-73 -0.44575681711994974 1107 74-75 -0.563929254559401 1107 76-77 -0.47671764871464717 1107 78-79 -0.6028963931095142 1107 80-81 -0.453707774052436 1107 82-83 -0.2686766769612525 1107 84-85 -0.39852552668457264 1107 86-87 -0.3096211125826507 1107 88-89 -0.39909725074885216 1107 90-91 -0.07327827412222376 1107 92-93 -0.1743402400649714 1107 94-95 -0.36196075677738193 1107 96-97 -0.22395704953262197 1107 98-99 0.04480628578749801 1107 100-101 0.2826936605501231 1108 1 -0.14351542860209676 1108 2 0.12912793361100583 1108 3 0.030367855034171498 1108 4 0.022210784586313537 1108 5 -0.04470090895382128 1108 6 -0.0914704403806823 1108 7 -0.10197601338989415 1108 8 0.15577268529291644 1108 9 0.10318235982177981 1108 10-11 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-0.5189258425083452 2316 96-97 -0.5246274418545589 2316 98-99 -0.37107562419604534 2316 100-101 0.0992463859050261 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4092.0 3 1180.0 4 1262.0 5 1334.0 6 1545.0 7 1761.0 8 2182.0 9 2615.0 10 3181.0 11 3763.0 12 4601.0 13 6122.0 14 5603.0 15 5703.0 16 6832.0 17 8425.0 18 7515.0 19 6239.0 20 6616.0 21 7297.0 22 7661.0 23 8652.0 24 9978.0 25 11233.0 26 12744.0 27 15035.0 28 17643.0 29 21403.0 30 25361.0 31 31250.0 32 39372.0 33 51222.0 34 70172.0 35 100927.0 36 154390.0 37 235982.0 38 209649.0 39 28278.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.20879217628021 19.63510320224541 15.11293963522646 24.043164986247916 2 15.10956289988986 19.50104469097452 40.73442171058588 24.65497069854974 3 19.43777317528579 23.46072429471108 28.981809998347323 28.119692531655804 4 12.961943860821254 15.744815521348887 36.04722512812849 35.24601548970137 5 14.443973611472538 36.74445960487541 33.69302237257619 15.118544411075868 6 33.67116988498739 34.57784634469696 17.185033161340087 14.56595060897557 7 29.88467976118826 31.482556990163737 19.94209825723662 18.69066499141139 8 26.71880683885049 35.141193196296236 19.208653827566632 18.931346137286635 9 26.68812916864729 16.794964278465418 19.73809186993513 36.77881468295216 10-11 25.78508570002706 25.962082526472646 26.860992693444487 21.39183908005581 12-13 26.14348763178543 23.02303186222179 27.566098096947684 23.2673824090451 14-15 22.866148581268973 24.703887397697173 25.549766510128148 26.880197510905713 16-17 23.03824016011963 28.0991768213125 26.019835715710332 22.842747302857536 18-19 22.870570021210828 26.625826598412438 28.096965287699298 22.406638092677433 20-21 23.390819848444373 26.053455693177185 28.00839847319659 22.54732598518185 22-23 23.12210832494848 25.76981717363891 28.020633323520492 23.087441177892114 24-25 22.996740438164558 26.062125895193088 28.06883484505876 22.872298821583588 26-27 22.7759817941898 26.459635189521553 27.820940460690665 22.943442555597983 28-29 22.54724309807864 26.526085642175712 28.14997926084236 22.776691998903285 30-31 22.805998505250734 26.069007151685415 28.103790608563305 23.021203734500546 32-33 22.540819392968046 26.28178429646188 28.009458184176257 23.167938126393818 34-35 23.197928621216494 26.392873738831664 27.612985897444926 22.796211742506916 36-37 23.077109468791647 26.13580342096858 27.725000826054874 23.062086284184904 38-39 22.7479877115518 26.364050783443627 27.874869242542694 23.013092262461875 40-41 22.834978203687957 26.251840212929643 27.92508533347543 22.98809624990697 42-43 22.876039246346807 26.1896025811851 27.640263844688416 23.294094327779675 44-45 22.82894131111764 26.456274859723866 27.561479426974696 23.1533044021838 46-47 22.842541643354025 26.709679408952937 27.21221920198073 23.235559745712308 48-49 23.141937439528814 26.497570578355678 27.330905380327415 23.029586601788097 50-51 22.904708952843507 26.6293477322271 27.262882610128454 23.203060704800944 52-53 23.144594237377742 26.579566481628337 27.189576174112258 23.086263106881663 54-55 22.813889542316094 26.47155477203026 27.34751431886601 23.36704136678763 56-57 22.924690380967608 26.95289774171116 27.179708739446134 22.942703137875103 58-59 23.0058879142974 26.504377295046993 27.296698302848664 23.19303648780694 60-61 23.02920153021811 26.463388814230626 27.658282981187437 22.84912667436383 62-63 22.889407959039758 26.589889701752938 27.422070837365474 23.09863150184183 64-65 22.693974300511744 26.427471532518688 27.426240089527447 23.452314077442125 66-67 22.658206933016093 26.80554729409691 27.196545608759347 23.339700164127645 68-69 22.600642660607868 26.547470691609277 27.50969895774762 23.342187690035235 70-71 22.68459457518163 26.603933969385423 27.388445215309847 23.323026240123106 72-73 22.81784067883086 26.43872427100539 27.40802945982825 23.335405590335498 74-75 22.69788816482272 26.806295410184216 27.054137531448415 23.441678893544648 76-77 22.734267308504766 26.686488051000488 27.130729063644445 23.448515576850305 78-79 22.838297928567968 26.64417440021846 27.227637298990047 23.289890372223518 80-81 22.678441734160717 26.971517062499146 27.14870684054726 23.201334362792878 82-83 22.88483665267042 26.826325517635098 27.068381926689728 23.22045590300476 84-85 22.842142267465373 26.64979740141656 26.957682050179603 23.550378280938464 86-87 22.789106956116477 26.544680386195424 27.327978024320903 23.338234633367193 88-89 22.786304708516425 26.60672604923249 27.106373155015284 23.500596087235802 90-91 22.670608604605967 26.80572147996297 27.2289407224252 23.294729193005868 92-93 22.86898869829452 26.42668168390476 27.292948948440817 23.4113806693599 94-95 22.775553261270456 26.46308044348149 27.260249043031948 23.50111725221611 96-97 22.830218195518874 26.415076047847457 27.283539601506195 23.471166155127474 98-99 22.62023888049498 26.351320090786395 27.07799901564351 23.95044201307511 100-101 22.84355575526099 25.874555618697713 27.653314302580167 23.62857432346113 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 891.0 1 536.0 2 209.5 3 261.0 4 229.0 5 191.5 6 373.0 7 835.0 8 1232.5 9 1304.0 10 1127.5 11 961.0 12 974.0 13 1046.5 14 1134.5 15 1212.0 16 1263.0 17 1295.5 18 1327.5 19 1396.5 20 1503.0 21 1675.0 22 1906.5 23 2257.0 24 2693.5 25 3336.0 26 4192.0 27 5258.0 28 6375.0 29 7616.5 30 9215.5 31 11131.0 32 13304.5 33 15470.0 34 17666.5 35 19883.0 36 22728.0 37 25624.5 38 28013.5 39 29545.0 40 30856.0 41 34229.5 42 37362.5 43 39476.0 44 41686.5 45 43552.5 46 44375.0 47 45145.5 48 48270.5 49 49114.5 50 47272.5 51 46067.0 52 44407.0 53 43324.0 54 42597.5 55 39670.5 56 35054.5 57 30992.5 58 27242.5 59 23476.5 60 20033.0 61 16606.0 62 13519.0 63 10738.5 64 8430.5 65 6560.0 66 5074.0 67 4286.0 68 3313.5 69 2231.5 70 1687.5 71 1310.5 72 1014.0 73 869.0 74 666.5 75 543.0 76 393.5 77 209.0 78 115.5 79 85.0 80 60.0 81 39.5 82 26.0 83 23.0 84 20.5 85 12.5 86 9.5 87 9.0 88 4.5 89 1.5 90 2.5 91 1.5 92 1.0 93 1.5 94 1.0 95 1.5 96 3.0 97 2.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26351629612523036 2 0.10291272877666444 3 0.11248396379087641 4 0.11406453471065453 5 0.11985996141650765 6 0.05321255429919681 7 0.06093978990700097 8 0.0652424551886192 9 0.06322283679112492 10-11 0.05909579050059315 12-13 0.06234474183569263 14-15 0.05830550504070409 16-17 0.05971045696939576 18-19 0.06418874124210044 20-21 0.06717426409057023 22-23 0.0758674041493499 24-25 0.08306778278389468 26-27 0.08346292551383921 28-29 0.07771140355575772 30-31 0.07424292848180016 32-33 0.06734988308165668 34-35 0.22663630799707418 36-37 0.3439058892950566 38-39 0.3175630406320879 40-41 0.3012304744610473 42-43 0.32414875279783 44-45 0.33679332015605506 46-47 0.3419301756453339 48-49 0.35044769671302717 50-51 0.3448278889982605 52-53 0.345003507989347 54-55 0.3326223691177516 56-57 0.3093089480510243 58-59 0.2956984762418238 60-61 0.2557451557696546 62-63 0.3230950388513113 64-65 0.3496135065053665 66-67 0.3548820762379602 68-69 0.36625340591080835 70-71 0.3664729296496665 72-73 0.3508428394429717 74-75 0.354223505021386 76-77 0.33890074804909254 78-79 0.3182216118486621 80-81 0.31659713618111235 82-83 0.33042713172917093 84-85 0.3359591299483944 86-87 0.32981246526036834 88-89 0.3449156984938037 90-91 0.3681413100649878 92-93 0.3629166450801657 94-95 0.36225807386359143 96-97 0.3671754056140123 98-99 0.35804321807751643 100-101 0.3357835109573079 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1138829.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.42645855278904 #Duplication Level Percentage of deduplicated Percentage of total 1 82.0205405353874 51.20251874209715 2 10.62214792371112 13.262061542022929 3 3.201356590121327 5.995480634577213 4 1.3142021551255068 3.2816394546771392 5 0.7256427185521 2.264965254691292 6 0.44459753058113766 1.665278958929745 7 0.3051975677120635 1.3336682317832411 8 0.20938152561824955 1.0456757704581914 9 0.16541839450955503 0.9293836093847679 >10 0.8762449894070453 10.066114136319557 >50 0.07273612510061454 3.196438360666612 >100 0.04056217442162055 4.486656128051206 >500 0.0012675662693491959 0.5006270656296167 >1k 7.042034829717755E-4 0.7694921107113463 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2802 0.2460422065121278 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2081 0.18273156022545967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1683 0.1477833809992545 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1182 0.10379082373209675 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 8.780949554322905E-5 0.0 0.0 0.0 0.0 18-19 1.756189910864581E-4 0.0 0.0 0.0 0.0 20-21 1.756189910864581E-4 0.0 0.0 1.3171424331484357E-4 0.0 22-23 1.756189910864581E-4 0.0 0.0 2.195237388580726E-4 0.0 24-25 1.756189910864581E-4 0.0 0.0 3.512379821729162E-4 0.0 26-27 1.756189910864581E-4 0.0 0.0 6.585712165742179E-4 0.0 28-29 1.756189910864581E-4 0.0 0.0 0.0011415234420619775 0.0 30-31 1.756189910864581E-4 0.0 0.0 0.002502570622982028 0.0 32-33 1.756189910864581E-4 0.0 0.0 0.006322283679112492 0.0 34-35 1.756189910864581E-4 0.0 0.0 0.012688472105996598 0.0 36-37 1.756189910864581E-4 0.0 0.0 0.02432323026547445 0.0 38-39 2.6342848662968713E-4 0.0 0.0 0.03925084450782339 0.0 40-41 2.6342848662968713E-4 4.3904747771614525E-5 0.0 0.057339600589728576 0.0 42-43 3.0733323440130167E-4 8.780949554322905E-5 0.0 0.07577959465380668 0.0 44-45 3.512379821729162E-4 8.780949554322905E-5 0.0 0.0991369204683056 0.0 46-47 4.3904747771614526E-4 8.780949554322905E-5 0.0 0.12613834034784854 0.0 48-49 4.3904747771614526E-4 8.780949554322905E-5 0.0 0.16178899553839954 0.0 50-51 4.3904747771614526E-4 8.780949554322905E-5 0.0 0.20055688782073516 0.0 52-53 4.3904747771614526E-4 8.780949554322905E-5 0.0 0.23791982817437912 0.0 54-55 4.3904747771614526E-4 8.780949554322905E-5 0.0 0.28072695725170327 0.0 56-57 4.3904747771614526E-4 8.780949554322905E-5 0.0 0.3353444634795918 0.0 58-59 4.3904747771614526E-4 8.780949554322905E-5 0.0 0.397733110063056 0.0 60-61 4.3904747771614526E-4 8.780949554322905E-5 0.0 0.46666356406449083 0.0 62-63 4.3904747771614526E-4 8.780949554322905E-5 0.0 0.5369989699946173 0.0 64-65 4.3904747771614526E-4 8.780949554322905E-5 0.0 0.6126029456573374 0.0 66-67 5.268569732593743E-4 8.780949554322905E-5 0.0 0.7053297729509873 0.0 68-69 5.268569732593743E-4 8.780949554322905E-5 0.0 0.813467166712474 0.0 70-71 5.707617210309887E-4 8.780949554322905E-5 0.0 0.9339417945977843 0.0 72-73 6.146664688026033E-4 8.780949554322905E-5 0.0 1.0580605165481385 0.0 74-75 6.146664688026033E-4 8.780949554322905E-5 0.0 1.1938579014057422 0.0 76-77 6.146664688026033E-4 8.780949554322905E-5 0.0 1.3448463289923245 0.0 78-79 6.146664688026033E-4 8.780949554322905E-5 0.0 1.5236703666661104 0.0 80-81 6.146664688026033E-4 8.780949554322905E-5 0.0 1.7248419209556483 0.0 82-83 6.146664688026033E-4 8.780949554322905E-5 0.0 1.9337846156007619 0.0 84-85 6.146664688026033E-4 8.780949554322905E-5 0.0 2.1483032132128708 0.0 86-87 6.146664688026033E-4 8.780949554322905E-5 0.0 2.381393519132372 0.0 88-89 6.146664688026033E-4 8.780949554322905E-5 0.0 2.63722648439757 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3700 0.0 36.83215 1 ATACCGT 95 8.858915E-7 34.919014 6 GGTATCA 2315 0.0 31.997828 1 ATCAACG 4580 0.0 29.404078 3 TCAACGC 4620 0.0 29.149494 4 TATCAAC 4655 0.0 28.930326 2 CAACGCA 4655 0.0 28.726591 5 AACGCAG 4815 0.0 28.050207 6 TACCGTG 140 5.9247213E-7 27.08005 7 GTACATG 4210 0.0 25.602987 1 TACATGG 4265 0.0 24.348923 2 ACGCAGA 5550 0.0 24.079287 7 CGCAGAG 5620 0.0 23.695042 8 ACATGGG 4250 0.0 23.430689 3 ACCGTGC 195 4.316571E-7 21.872347 8 TAAGGCG 240 6.044502E-9 21.733822 5 GCAGAGT 6045 0.0 21.558765 9 GAGTACT 2780 0.0 20.711853 12-13 AGAGTAC 5215 0.0 19.946547 10-11 AGTACTT 2930 0.0 19.732391 12-13 >>END_MODULE