FastQCFastQC Report
Fri 27 May 2016
SRR939342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939342_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138829
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33900.2976741898915465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27540.24182735072605283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21470.18852698693131278No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC14240.12504072165355817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.035.176681
GTATCAA41800.035.1627961
ATCAACG53050.027.5508923
TATCAAC53600.027.5337852
TCAACGC54200.026.966324
CAACGCA54400.026.8671845
AACGCAG56550.025.929626
GTACATG44500.023.9435861
TGCACCG2602.5465852E-1123.7268645
TACATGG45200.022.7819862
ACGCAGA64850.022.610957
CGCAGAG65400.022.3482368
GTACCTG8400.022.0844461
ACATGGG45150.021.7561933
AGTACTT35050.021.52963312-13
CATGGGG28900.021.1817654
TACGACA1353.8209814E-421.0905444
GCAGAGT69500.020.895139
GAGTACT34450.020.59583912-13
AGAGTAC58950.020.32804110-11
Produced by FastQC (version 0.11.5)