##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939342_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1138829 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.129663013498956 33.0 31.0 34.0 30.0 34.0 2 32.355490596042074 34.0 31.0 34.0 30.0 34.0 3 32.44108817039257 34.0 31.0 34.0 30.0 34.0 4 36.01466418575572 37.0 35.0 37.0 35.0 37.0 5 35.93798717805746 37.0 35.0 37.0 35.0 37.0 6 35.97688766267807 37.0 35.0 37.0 35.0 37.0 7 35.9255463287289 37.0 35.0 37.0 35.0 37.0 8 35.89693360460613 37.0 35.0 37.0 35.0 37.0 9 37.590007806264154 39.0 37.0 39.0 35.0 39.0 10-11 37.48578539886146 39.0 37.0 39.0 34.5 39.0 12-13 37.38765872663938 39.0 37.0 39.0 34.0 39.0 14-15 38.63115709206562 40.0 38.0 41.0 34.0 41.0 16-17 38.56066231190108 40.0 38.0 41.0 34.0 41.0 18-19 38.68313943533226 40.0 38.0 41.0 34.0 41.0 20-21 38.686140763889924 40.0 38.0 41.0 34.0 41.0 22-23 38.625398984395375 40.0 38.0 41.0 34.0 41.0 24-25 38.54229607781326 40.0 38.0 41.0 34.0 41.0 26-27 38.36536521286339 40.0 38.0 41.0 34.0 41.0 28-29 38.201448593247974 40.0 38.0 41.0 33.0 41.0 30-31 38.00462756041513 40.0 37.5 41.0 33.0 41.0 32-33 37.806227273804936 40.0 37.0 41.0 33.0 41.0 34-35 37.76717048828226 40.0 37.0 41.0 33.0 41.0 36-37 37.69617036447087 40.0 37.0 41.0 33.0 41.0 38-39 37.58413115577493 40.0 37.0 41.0 32.0 41.0 40-41 37.4144458913498 40.0 37.0 41.0 32.0 41.0 42-43 37.24020989981815 40.0 36.0 41.0 31.0 41.0 44-45 37.00795597934369 40.0 36.0 41.0 31.0 41.0 46-47 36.7239207993474 39.0 36.0 41.0 30.5 41.0 48-49 36.41875382520115 39.0 35.0 41.0 30.0 41.0 50-51 36.166283085520305 39.0 35.0 40.0 29.5 41.0 52-53 35.91751219893417 39.0 35.0 40.0 29.0 41.0 54-55 35.59899027861075 38.0 35.0 40.0 28.0 41.0 56-57 35.32384756622812 38.0 34.0 40.0 28.0 41.0 58-59 34.99250809384026 38.0 34.0 40.0 27.0 41.0 60-61 34.62834982249311 37.5 34.0 40.0 26.0 41.0 62-63 34.20269417094225 37.0 33.0 40.0 26.0 41.0 64-65 33.7374092159578 36.0 33.0 39.0 25.5 40.5 66-67 33.331625292295854 36.0 32.0 39.0 24.5 40.0 68-69 32.91620778887787 35.0 32.0 38.5 23.0 40.0 70-71 32.458458644800935 35.0 31.5 38.0 22.0 40.0 72-73 31.9090513149911 35.0 31.0 37.0 21.0 39.0 74-75 31.348085621282912 34.5 30.5 36.5 19.5 39.0 76-77 29.39945329808075 32.5 28.5 35.0 16.5 37.0 78-79 30.403849919522596 34.0 30.0 35.5 18.0 37.0 80-81 30.4331198977195 34.0 30.0 35.0 17.5 37.0 82-83 30.089315867439275 34.0 30.0 35.0 13.5 37.0 84-85 29.671640781890872 34.0 29.5 35.0 7.5 36.0 86-87 29.060363759616237 34.0 29.0 35.0 4.0 36.0 88-89 28.49602618127919 33.5 29.0 35.0 2.0 35.0 90-91 27.995699969003248 33.0 27.5 35.0 2.0 35.0 92-93 27.547355660946465 33.0 27.0 35.0 2.0 35.0 94-95 27.028392761336427 33.0 25.5 35.0 2.0 35.0 96-97 26.32589352747427 32.5 25.0 34.0 2.0 35.0 98-99 25.397195276902856 32.0 22.0 34.0 2.0 35.0 100-101 21.903905678552267 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14065942126636344 1101 2 0.197122622065109 1101 3 0.28956459360949083 1101 4 0.23358147475924085 1101 5 0.24698721156826764 1101 6 0.02170437618848098 1101 7 0.1074477900358346 1101 8 0.18142250401079707 1101 9 0.15218316404244803 1101 10-11 0.20097635303478256 1101 12-13 0.18995014362095475 1101 14-15 0.14386669934422258 1101 16-17 0.3094885710366526 1101 18-19 0.18483722393423108 1101 20-21 0.1951918747063104 1101 22-23 0.1748739992924655 1101 24-25 0.20539267673502337 1101 26-27 0.09110307282194441 1101 28-29 0.211649911643768 1101 30-31 0.19326975691128467 1101 32-33 0.17028835595927916 1101 34-35 0.2780115329271311 1101 36-37 0.19035496589595624 1101 38-39 0.22077035331192718 1101 40-41 0.1374262277565137 1101 42-43 0.17232067772327753 1101 44-45 0.3464203693458714 1101 46-47 0.28321233490861175 1101 48-49 0.33557939290188443 1101 50-51 0.33525050699325476 1101 52-53 0.5444390676071365 1101 54-55 0.2997801740124899 1101 56-57 0.2971089459318179 1101 58-59 0.26346237973103115 1101 60-61 0.06535008249068852 1101 62-63 0.14057736582608982 1101 64-65 0.2143550886824812 1101 66-67 0.10481273757783072 1101 68-69 0.14749124372408318 1101 70-71 0.02156103738487758 1101 72-73 0.05270698582949507 1101 74-75 -0.032792906506461605 1101 76-77 0.22561784338954993 1101 78-79 0.2934980361425872 1101 80-81 0.08766332325345871 1101 82-83 -0.03448338454363409 1101 84-85 0.15642645571839964 1101 86-87 0.3156539437064616 1101 88-89 0.5058715615490534 1101 90-91 0.5848019859710263 1101 92-93 0.48633561025012995 1101 94-95 0.7109586609479628 1101 96-97 0.5009059136852478 1101 98-99 0.29175268479018257 1101 100-101 0.1291730090480776 1102 1 0.16835010622071422 1102 2 0.14703689856237645 1102 3 0.19903296290561912 1102 4 0.17581053955751003 1102 5 0.12380362553485469 1102 6 0.12623992878791768 1102 7 0.12346884213324927 1102 8 0.2125249118806849 1102 9 0.3648399764330321 1102 10-11 0.26649865020717556 1102 12-13 0.27286814990613806 1102 14-15 0.37216170780783386 1102 16-17 0.42196985866528536 1102 18-19 0.2669111346295523 1102 20-21 0.29671305910426327 1102 22-23 0.26475364204475227 1102 24-25 0.27403808228149273 1102 26-27 0.2751118336551812 1102 28-29 0.14770525314228422 1102 30-31 0.22686775174249618 1102 32-33 0.12468272756795074 1102 34-35 0.3291724248865151 1102 36-37 0.25432046626387006 1102 38-39 0.14140769758694205 1102 40-41 0.30785515336736324 1102 42-43 0.3578681119988971 1102 44-45 0.5107634699631518 1102 46-47 0.4741981370667503 1102 48-49 0.5120642399588249 1102 50-51 0.43879063392927975 1102 52-53 0.331016996251833 1102 54-55 0.24910272273714895 1102 56-57 0.3964254233301787 1102 58-59 0.42845203129126475 1102 60-61 0.3641694360666179 1102 62-63 0.2911290409684426 1102 64-65 0.38642735015186247 1102 66-67 0.2878627725559113 1102 68-69 0.6157962019134828 1102 70-71 0.3803021161598892 1102 72-73 0.4271868453371894 1102 74-75 0.32495999594997826 1102 76-77 0.47847181983642884 1102 78-79 0.6620646956807299 1102 80-81 0.6335906413220869 1102 82-83 0.6791217327688877 1102 84-85 0.3078933606468688 1102 86-87 0.35625571182167093 1102 88-89 0.6221451626973469 1102 90-91 0.6090641838998856 1102 92-93 0.8548660230640763 1102 94-95 0.8388380101017852 1102 96-97 0.6865022810385462 1102 98-99 0.8019081107435646 1102 100-101 0.5813454564631435 1103 1 0.20618477686287662 1103 2 0.04277698163200938 1103 3 0.18121671236358594 1103 4 0.09682360922777633 1103 5 0.1429754019277425 1103 6 0.13092737839245672 1103 7 0.1389431597115447 1103 8 0.07103506981333396 1103 9 0.10869576210047427 1103 10-11 0.1413324571831609 1103 12-13 0.10787565770959162 1103 14-15 0.27455981490960824 1103 16-17 0.18726544034982595 1103 18-19 0.08244986880313832 1103 20-21 0.22082914515551977 1103 22-23 0.2782797831771262 1103 24-25 0.17368661315308032 1103 26-27 0.23936779062238855 1103 28-29 0.2087478164748191 1103 30-31 0.4179449333955034 1103 32-33 0.22456876663719783 1103 34-35 0.3833697170601269 1103 36-37 0.3851399098621684 1103 38-39 0.3810360881934045 1103 40-41 0.31571550105571333 1103 42-43 0.45392633600022236 1103 44-45 0.4148046930004625 1103 46-47 0.4073105964368082 1103 48-49 0.4620150539910739 1103 50-51 0.4169629048695995 1103 52-53 0.5962014839472829 1103 54-55 0.39944962267730233 1103 56-57 0.3271972936153986 1103 58-59 0.23262020608870415 1103 60-61 0.29243004464665034 1103 62-63 0.1848954138930452 1103 64-65 0.4759762727193504 1103 66-67 0.4387190025993135 1103 68-69 0.2616545463263549 1103 70-71 0.37602141390252797 1103 72-73 0.35837263032374267 1103 74-75 0.49729589041785616 1103 76-77 0.698765376743431 1103 78-79 0.5545051080902859 1103 80-81 0.483763374556748 1103 82-83 0.3631135368994336 1103 84-85 0.450839967442235 1103 86-87 0.4969472898221916 1103 88-89 0.7101875970175051 1103 90-91 0.7460605428839848 1103 92-93 0.6906485010334222 1103 94-95 0.5814735537276796 1103 96-97 0.30438330654357415 1103 98-99 0.3499374467240415 1103 100-101 0.47105958654345415 1104 1 0.05031066407418194 1104 2 -0.023178680007092112 1104 3 0.08934253748842735 1104 4 0.047518330929499086 1104 5 0.014928098938021606 1104 6 0.014916226973049618 1104 7 0.0039352738678601895 1104 8 0.07336158771967405 1104 9 0.09315862361402338 1104 10-11 0.08398827481450866 1104 12-13 0.16482317653601797 1104 14-15 0.22325655959539148 1104 16-17 0.03350011167527356 1104 18-19 0.18073145335326757 1104 20-21 0.13197521822957015 1104 22-23 0.1758953179011371 1104 24-25 0.16009183255544457 1104 26-27 0.08802210955540346 1104 28-29 0.17082333259013893 1104 30-31 0.10168207308919364 1104 32-33 0.06949746636548326 1104 34-35 0.2088243926454041 1104 36-37 0.2349829259537728 1104 38-39 0.33695254050366685 1104 40-41 0.27506395531825234 1104 42-43 0.23441876791852678 1104 44-45 0.2194345326190401 1104 46-47 0.2866740031902637 1104 48-49 0.22140757239927922 1104 50-51 0.26470015493923427 1104 52-53 0.3346553411984132 1104 54-55 0.4633184891594908 1104 56-57 0.5774264530422428 1104 58-59 0.41748524994158487 1104 60-61 0.33761304019797933 1104 62-63 0.39225041827789653 1104 64-65 0.2708908736456408 1104 66-67 0.28963128563082563 1104 68-69 0.4818614494792257 1104 70-71 0.12263058735056376 1104 72-73 0.2533546640522104 1104 74-75 -0.04402610293716691 1104 76-77 -0.055076007853781306 1104 78-79 0.15098920317571185 1104 80-81 0.19635002613870256 1104 82-83 0.3591895859235983 1104 84-85 0.3385246854618842 1104 86-87 0.29198045865512867 1104 88-89 0.6448079598266787 1104 90-91 0.5006448093108133 1104 92-93 0.5119640787868036 1104 94-95 0.758314352815006 1104 96-97 0.442011327546048 1104 98-99 0.8567341902888188 1104 100-101 0.5229388663059389 1105 1 -0.060579502467007273 1105 2 -0.03324525597020056 1105 3 -0.014978654396870184 1105 4 0.0130630755809662 1105 5 0.00540675715552652 1105 6 0.016784033848743718 1105 7 -0.0020555499928036625 1105 8 0.074162558675404 1105 9 0.07869725759139357 1105 10-11 -0.008116210960444903 1105 12-13 0.025602355004842536 1105 14-15 0.0260383511370037 1105 16-17 -0.1459047221717853 1105 18-19 0.03307258426686133 1105 20-21 -0.13967589604003905 1105 22-23 0.08535015124178358 1105 24-25 0.18910234223968558 1105 26-27 0.1325245285045682 1105 28-29 0.1154191840838692 1105 30-31 0.021092072312697496 1105 32-33 0.022933116915943685 1105 34-35 0.0643719394291864 1105 36-37 0.049583048167257004 1105 38-39 -0.13443659127420204 1105 40-41 0.10381400173911715 1105 42-43 -0.12031261562586337 1105 44-45 0.008355475350889208 1105 46-47 -0.22736662097626947 1105 48-49 0.025822956443327882 1105 50-51 0.15731307580769283 1105 52-53 0.09357785379957306 1105 54-55 0.10391801043375182 1105 56-57 0.20250030442119282 1105 58-59 -0.04591063058629885 1105 60-61 -0.09838651078563032 1105 62-63 0.005372162487105925 1105 64-65 -0.16319698164279117 1105 66-67 0.03636325661018702 1105 68-69 0.03770912826708184 1105 70-71 0.06886931552009656 1105 72-73 0.05358288591357052 1105 74-75 -0.010510659136802047 1105 76-77 0.15199650660420616 1105 78-79 0.10626691812120725 1105 80-81 0.10689574984845507 1105 82-83 0.24240481331832697 1105 84-85 0.1485886449761331 1105 86-87 0.11407248675747539 1105 88-89 0.2816077221518434 1105 90-91 0.6567066992984252 1105 92-93 0.48765473688277083 1105 94-95 0.5482949573328533 1105 96-97 0.5543063583188648 1105 98-99 0.7257695309167005 1105 100-101 0.4735732285507517 1106 1 0.11153576850776403 1106 2 0.15686058636936195 1106 3 0.1757017043640161 1106 4 0.09623663863332155 1106 5 0.1698267910355824 1106 6 -0.002953067436862966 1106 7 0.08729980975296314 1106 8 0.13782226110873097 1106 9 0.12004274222061895 1106 10-11 0.20474540608989145 1106 12-13 0.2422131020731655 1106 14-15 0.18359865894648664 1106 16-17 0.13756496305888533 1106 18-19 0.1595203656485893 1106 20-21 0.20542955234540727 1106 22-23 0.19074108504435827 1106 24-25 0.21907076025126315 1106 26-27 0.1666639357349382 1106 28-29 0.26258895204490074 1106 30-31 0.18626554344242408 1106 32-33 0.13743423968518442 1106 34-35 0.2540181686316956 1106 36-37 0.24755484105244108 1106 38-39 0.42874380031619097 1106 40-41 0.2769108750566076 1106 42-43 0.27231847260672737 1106 44-45 0.40489327524846175 1106 46-47 0.44551357757660526 1106 48-49 0.26934405268632844 1106 50-51 0.07511290772060164 1106 52-53 0.11116462219394663 1106 54-55 0.0739643657015776 1106 56-57 0.07693399537732404 1106 58-59 0.23208145093594368 1106 60-61 0.19475310655360545 1106 62-63 0.10232919522469786 1106 64-65 0.12138398249425109 1106 66-67 -0.14054676426938784 1106 68-69 0.13007167110959017 1106 70-71 0.1046485567644595 1106 72-73 0.217137862210663 1106 74-75 0.1423862168070471 1106 76-77 0.14915839548288545 1106 78-79 0.4605826854781654 1106 80-81 0.5381868688094684 1106 82-83 0.41108119314262126 1106 84-85 0.4024937151656296 1106 86-87 0.38667396277223887 1106 88-89 0.37864354571292225 1106 90-91 0.45054558519618126 1106 92-93 0.5409173335656767 1106 94-95 0.3156978804724062 1106 96-97 0.3867461818274194 1106 98-99 0.30784494686658803 1106 100-101 0.33970269500555617 1107 1 -0.037525143066787336 1107 2 -0.11400248368766341 1107 3 -0.0630311721271255 1107 4 -0.04563094602349338 1107 5 -0.0038367517949922103 1107 6 -0.07131308577944395 1107 7 -0.017394986508762145 1107 8 0.09253740213345907 1107 9 -0.16484852632379443 1107 10-11 -0.12577464480430933 1107 12-13 -0.17690516235708031 1107 14-15 -0.078663097420133 1107 16-17 -0.19519785575646864 1107 18-19 -0.04879466816247202 1107 20-21 -0.07449501056512275 1107 22-23 -0.0762362967461101 1107 24-25 -0.09757367787805293 1107 26-27 -0.16627972541818536 1107 28-29 -0.2583435485525243 1107 30-31 -0.14390816208166513 1107 32-33 -0.09204903562790179 1107 34-35 0.03240557167114133 1107 36-37 0.0035613901285316274 1107 38-39 -0.1771256638092069 1107 40-41 0.03063106029082263 1107 42-43 -0.14622658619933304 1107 44-45 -0.28028443464167907 1107 46-47 -0.33699202530654304 1107 48-49 -0.45280864929195985 1107 50-51 -0.44772489607837684 1107 52-53 -0.4799290541370169 1107 54-55 -0.3773438348525815 1107 56-57 -0.45395290711636704 1107 58-59 -0.44837578953916335 1107 60-61 -0.43133114014118235 1107 62-63 -0.3918911159532996 1107 64-65 -0.46817334129749355 1107 66-67 -0.48483101613108914 1107 68-69 -0.402928826206832 1107 70-71 -0.3730343684895274 1107 72-73 -0.3963253654375478 1107 74-75 -0.21084165745585892 1107 76-77 -0.15894770084149457 1107 78-79 0.061587167784679764 1107 80-81 -0.24877843529630184 1107 82-83 0.1591847705915832 1107 84-85 0.06982293218289115 1107 86-87 -0.004433115267687526 1107 88-89 0.13637697764838919 1107 90-91 0.015462333134252049 1107 92-93 0.015458930532695092 1107 94-95 0.24530257849799497 1107 96-97 -0.007832423232290608 1107 98-99 0.11849978988898258 1107 100-101 0.03792285117583205 1108 1 -0.037344613427976014 1108 2 -0.03528517073554127 1108 3 -0.09682536194844715 1108 4 -0.0265203660280946 1108 5 -0.006608600674105958 1108 6 -0.03448344185418506 1108 7 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-0.37454933197722795 2316 52-53 -0.3783178704024266 2316 54-55 -0.44857971734072777 2316 56-57 -0.26402486782354373 2316 58-59 -0.09694968992793918 2316 60-61 -0.18013179502169407 2316 62-63 -0.24506723001870512 2316 64-65 -0.21331532373530138 2316 66-67 -0.10696256475991106 2316 68-69 0.0735006225456658 2316 70-71 0.05669606441694697 2316 72-73 0.028430711314904045 2316 74-75 0.07617904517283236 2316 76-77 0.08344410779350753 2316 78-79 0.13816158147343316 2316 80-81 -0.032487366010673924 2316 82-83 0.13661085328082123 2316 84-85 0.05092989740963816 2316 86-87 0.030030606128882198 2316 88-89 0.1206395390434345 2316 90-91 -0.16832620138023202 2316 92-93 -0.1712218518831179 2316 94-95 0.025623235860393834 2316 96-97 0.3389986545425252 2316 98-99 0.35719555671542125 2316 100-101 1.0511177627373485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 21.0 5 28.0 6 59.0 7 155.0 8 356.0 9 640.0 10 1089.0 11 1610.0 12 2213.0 13 3179.0 14 3996.0 15 5122.0 16 6543.0 17 9223.0 18 8709.0 19 6996.0 20 7073.0 21 7458.0 22 8132.0 23 8998.0 24 10375.0 25 12037.0 26 14556.0 27 17598.0 28 21344.0 29 25876.0 30 30721.0 31 37707.0 32 46792.0 33 59194.0 34 78753.0 35 110735.0 36 165971.0 37 246627.0 38 169317.0 39 9619.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.732864380890334 20.439576886337722 15.553873039489726 22.273685693282214 2 16.601526655889515 20.77721940695223 39.54711374578624 23.074140191372013 3 19.94478538920242 24.103969955103004 29.47676955890656 26.474475096788012 4 13.90788257060542 16.96830691877358 35.814771137721294 33.309039372899704 5 14.473726959885989 37.13709433110678 33.59731794676813 14.791860762239107 6 33.26812014797656 35.625190436843454 16.75211994074615 14.354569474433825 7 29.751613279956867 32.06065177476162 19.803499910873363 18.38423503440815 8 26.50608651518358 36.2919279365032 18.796588425479154 18.40539712283407 9 26.384909411333922 17.3772357395184 19.559740751245357 36.67811409790232 10-11 25.763964563599977 26.551923071857143 26.63055647511611 21.05355588942677 12-13 26.317515623504494 23.48974253377812 27.479235249541418 22.71350659317597 14-15 22.848524762908323 25.19502985598876 25.574639971900247 26.38180540920267 16-17 22.922384372995463 28.577957866166265 26.01342525861119 22.48623250222708 18-19 22.72549258931762 26.892097057591613 28.449574080041867 21.932836273048895 20-21 23.353110958712854 26.150545867729047 28.41914808983614 22.07719508372196 22-23 23.09877075487189 25.97558544785916 28.366594106753517 22.559049690515433 24-25 23.009117259922256 26.19546042469941 28.455764649477665 22.339657665900674 26-27 22.76175824407412 26.533300316644098 28.39343073256657 22.311510706715207 28-29 22.505881040964006 26.699925976595257 28.558940806740956 22.235252175699777 30-31 22.778880762607905 26.289197061191803 28.375858008533328 22.556064167666964 32-33 22.707491642731263 26.413403592637703 28.26934509043939 22.609759674191647 34-35 23.250853542023773 26.425599586927813 28.044431604237502 22.279115266810916 36-37 23.151211534897772 26.223589206716685 28.14577941097684 22.479419847408696 38-39 22.810316809711757 26.399667703921033 28.337241881073304 22.452773605293906 40-41 22.904383964195475 26.287317971556973 28.39793013866701 22.410367925580545 42-43 23.038001138949664 26.302009583159354 27.90185186892683 22.758137408964156 44-45 23.058043877892853 26.42161466325436 27.907363721818957 22.61297773703383 46-47 23.06724714597187 26.81315632109825 27.51492980947974 22.60466672345014 48-49 23.274214126967262 26.660104370366405 27.476381440936258 22.589300061730075 50-51 23.024659540633404 26.772149286679564 27.455570590492517 22.747620582194518 52-53 23.434993313306915 26.780052141278453 27.149554498524363 22.63540004689027 54-55 22.966793083070417 26.59393991547458 27.40240194094109 23.036865060513914 56-57 23.100526944782754 27.03399720238947 27.306777400294514 22.55869845253326 58-59 23.20465639463972 26.58113083618936 27.441318697143803 22.77289407202712 60-61 23.225436226346833 26.589764146677076 27.76806483310078 22.41673479387531 62-63 23.01138596320999 26.708871374574173 27.518397421177898 22.76134524103794 64-65 22.823883129073813 26.48624156919081 27.53165751838072 23.158217783354658 66-67 22.75394286587363 26.889638391716403 27.35270176646362 23.003716975946347 68-69 22.796762274065177 26.616448195682562 27.563960717371604 23.022828812880654 70-71 22.872188393599213 26.754643038877234 27.473495789752363 22.899672777771194 72-73 22.947518898798677 26.60539905464297 27.41636365073246 23.03071839582589 74-75 22.83143474569053 26.923708475987173 27.056169100014138 23.188687678308156 76-77 22.851938143105123 26.80465444753186 27.24814259910722 23.0952648102558 78-79 22.980930411852878 26.751162817244733 27.28983016765467 22.97807660324772 80-81 22.823511333077217 27.089841391800668 27.221996597332748 22.864650677789363 82-83 23.075589048048478 26.89982429319942 27.105035084283948 22.919551574468162 84-85 22.842762170615604 26.806658418428054 27.12123593621167 23.22934347474467 86-87 22.929477781176498 26.653578673867262 27.328766160023342 23.088177384932898 88-89 22.77432128441159 26.75621045359904 27.192504015768776 23.276964246220597 90-91 22.77592911838993 26.976054855620262 27.27088090516708 22.977135120822727 92-93 22.908619292273027 26.56649944811732 27.34018891334871 23.18469234626094 94-95 22.766290095479697 26.582755875884732 27.263674908030488 23.38727912060508 96-97 22.727556112462892 26.56491887719754 27.43151078871367 23.2760142216259 98-99 22.65243508902566 26.49787632735029 27.145207928494976 23.704480655129085 100-101 22.78810299567668 26.094319186143256 27.657905146930013 23.459672671250047 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 284.0 1 195.5 2 177.0 3 245.0 4 192.5 5 159.0 6 337.5 7 833.0 8 1313.0 9 1403.0 10 1172.0 11 931.0 12 869.0 13 976.5 14 1086.0 15 1141.0 16 1187.0 17 1247.5 18 1303.5 19 1337.0 20 1455.5 21 1650.5 22 1951.5 23 2338.0 24 2810.0 25 3484.5 26 4399.0 27 5489.0 28 6608.5 29 7984.5 30 9654.5 31 11655.5 32 13951.0 33 16034.0 34 18263.0 35 20596.5 36 23395.5 37 26304.0 38 28683.0 39 30148.0 40 31373.0 41 34855.0 42 38427.0 43 40620.5 44 42491.5 45 44365.5 46 45119.5 47 45606.0 48 49001.5 49 49792.0 50 47552.5 51 45972.0 52 43853.5 53 42806.0 54 42326.5 55 38909.5 56 33957.0 57 29796.0 58 26163.5 59 22660.5 60 18883.5 61 15402.5 62 12641.0 63 10073.0 64 7986.5 65 6512.5 66 4833.5 67 3813.5 68 2831.5 69 1779.5 70 1272.5 71 983.0 72 777.0 73 627.5 74 511.5 75 403.0 76 294.0 77 174.0 78 101.0 79 68.5 80 38.5 81 24.5 82 15.0 83 9.5 84 7.5 85 4.0 86 2.0 87 2.5 88 2.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2602673447901309 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0025464753707536425 16-17 4.829522254877598E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.659044509755196E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0039514272994453075 36-37 0.02037180296602914 38-39 0.007507711868946085 40-41 0.01773751809973227 42-43 0.005312474480365357 44-45 8.34190207660676E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.024759643458324E-4 60-61 0.0024586658752104138 62-63 0.0012732376853768212 64-65 0.0 66-67 0.0 68-69 4.390474777161453E-4 70-71 4.3904747771614525E-5 72-73 0.0 74-75 0.0 76-77 0.2192164056236713 78-79 0.0 80-81 0.0014488566764632794 82-83 0.0 84-85 0.0 86-87 0.10054187239699726 88-89 0.3449156984938037 90-91 0.344432746268316 92-93 0.0 94-95 4.3904747771614525E-5 96-97 0.0 98-99 0.0 100-101 3.9514272994453073E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1138829.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.16870274873808 #Duplication Level Percentage of deduplicated Percentage of total 1 81.23478759426305 49.69026575210352 2 11.110766490915871 13.592623455869452 3 3.2966116297827317 6.049483705806386 4 1.3943006147762338 3.411502393905208 5 0.7613520284905677 2.328545795894415 6 0.45586606701042487 1.6730841567718195 7 0.3088172011128879 1.3222963305000055 8 0.21451830914119954 1.049744534881595 9 0.16250049450742596 0.8945950000542914 >10 0.9376048317163882 10.482731597261802 >50 0.07825626673923716 3.3278905162844477 >100 0.04260992553463277 4.668075285607972 >500 0.001434675721053266 0.6565997648655453 >1k 5.738702884213063E-4 0.8525617101936344 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3390 0.2976741898915465 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2754 0.24182735072605283 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2147 0.18852698693131278 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1424 0.12504072165355817 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 8.780949554322905E-5 0.0 6 0.0 0.0 0.0 8.780949554322905E-5 0.0 7 0.0 0.0 0.0 8.780949554322905E-5 0.0 8 0.0 0.0 0.0 8.780949554322905E-5 0.0 9 0.0 0.0 0.0 8.780949554322905E-5 0.0 10-11 0.0 0.0 0.0 8.780949554322905E-5 0.0 12-13 4.3904747771614525E-5 0.0 0.0 8.780949554322905E-5 0.0 14-15 8.780949554322905E-5 0.0 0.0 8.780949554322905E-5 0.0 16-17 8.780949554322905E-5 0.0 0.0 8.780949554322905E-5 0.0 18-19 8.780949554322905E-5 0.0 0.0 8.780949554322905E-5 0.0 20-21 8.780949554322905E-5 0.0 0.0 1.3171424331484357E-4 0.0 22-23 8.780949554322905E-5 0.0 0.0 2.195237388580726E-4 0.0 24-25 8.780949554322905E-5 0.0 0.0 3.512379821729162E-4 0.0 26-27 8.780949554322905E-5 0.0 0.0 7.024759643458324E-4 0.0 28-29 1.3171424331484357E-4 0.0 0.0 0.0012293329376052067 0.0 30-31 1.756189910864581E-4 0.0 0.0 0.002590380118525257 0.0 32-33 1.756189910864581E-4 0.0 0.0 0.006366188426884106 0.0 34-35 1.756189910864581E-4 0.0 0.0 0.01290799584485467 0.0 36-37 1.756189910864581E-4 0.0 0.0 0.024806182490962208 0.0 38-39 1.756189910864581E-4 0.0 0.0 0.039865510976625995 0.0 40-41 1.756189910864581E-4 0.0 0.0 0.05795426705853117 0.0 42-43 1.756189910864581E-4 0.0 0.0 0.07652597536592412 0.0 44-45 1.756189910864581E-4 0.0 0.0 0.10010282491928112 0.0 46-47 1.756189910864581E-4 0.0 0.0 0.12675300681665114 0.0 48-49 1.756189910864581E-4 0.0 0.0 0.16178899553839954 0.0 50-51 1.756189910864581E-4 0.0 0.0 0.19994222135193257 0.0 52-53 1.756189910864581E-4 0.0 0.0 0.2372173522100333 0.0 54-55 1.756189910864581E-4 0.0 0.0 0.28055133826061684 0.0 56-57 1.756189910864581E-4 0.0 0.0 0.33534446347959174 0.0 58-59 1.756189910864581E-4 0.0 0.0 0.3979526338019141 0.0 60-61 1.756189910864581E-4 0.0 0.0 0.4663562308300895 0.0 62-63 1.756189910864581E-4 0.0 0.0 0.5372184937334754 0.0 64-65 1.756189910864581E-4 0.0 0.0 0.6130858978828253 0.0 66-67 1.756189910864581E-4 0.0 0.0 0.7055492966898455 0.0 68-69 1.756189910864581E-4 0.0 0.0 0.8141257379290482 0.0 70-71 1.756189910864581E-4 0.0 0.0 0.934073508841099 0.0 72-73 1.756189910864581E-4 0.0 0.0 1.057314135836021 0.0 74-75 1.756189910864581E-4 0.0 0.0 1.1931554254413963 0.0 76-77 1.756189910864581E-4 0.0 0.0 1.3438365197935775 0.0 78-79 1.756189910864581E-4 0.0 0.0 1.521431224529758 0.0 80-81 2.6342848662968713E-4 0.0 0.0 1.7215929696205488 0.0 82-83 2.6342848662968713E-4 0.0 0.0 1.9289111885981125 0.0 84-85 2.6342848662968713E-4 0.0 0.0 2.1411467393260972 0.0 86-87 3.0733323440130167E-4 0.0 0.0 2.3735784740290247 0.0 88-89 3.512379821729162E-4 0.0 0.0 2.6281821063566175 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2745 0.0 35.17668 1 GTATCAA 4180 0.0 35.162796 1 ATCAACG 5305 0.0 27.550892 3 TATCAAC 5360 0.0 27.533785 2 TCAACGC 5420 0.0 26.96632 4 CAACGCA 5440 0.0 26.867184 5 AACGCAG 5655 0.0 25.92962 6 GTACATG 4450 0.0 23.943586 1 TGCACCG 260 2.5465852E-11 23.726864 5 TACATGG 4520 0.0 22.781986 2 ACGCAGA 6485 0.0 22.61095 7 CGCAGAG 6540 0.0 22.348236 8 GTACCTG 840 0.0 22.084446 1 ACATGGG 4515 0.0 21.756193 3 AGTACTT 3505 0.0 21.529633 12-13 CATGGGG 2890 0.0 21.181765 4 TACGACA 135 3.8209814E-4 21.090544 4 GCAGAGT 6950 0.0 20.89513 9 GAGTACT 3445 0.0 20.595839 12-13 AGAGTAC 5895 0.0 20.328041 10-11 >>END_MODULE