FastQCFastQC Report
Fri 27 May 2016
SRR939341_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939341_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885068
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115970.4019662621470274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93960.32567689912334824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63870.22138126380383408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147600.032.9879881
GGTATCA97050.032.7133751
TATCAAC181750.026.7151762
ATCAACG182650.026.6094973
TCAACGC184050.026.3288864
CAACGCA186000.026.0269135
GTACATG140050.025.4478821
AACGCAG190800.025.3305766
TACATGG138400.025.1131042
ACATGGG142600.023.608663
ACGCAGA210100.022.8909237
CGCAGAG211700.022.6955328
GAGTACT102500.022.32608812-13
CATGGGG66500.021.7468134
AGTACTT107550.020.9253412-13
GCAGAGT225200.020.9134759
GTACTTT110000.020.8510914-15
ATGGGGA40150.018.8948865
AGAGTAC197550.018.86235610-11
CATGGGA77500.018.843714
Produced by FastQC (version 0.11.5)