##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939341_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2885068 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.949131181656725 33.0 31.0 34.0 30.0 34.0 2 32.01281494924903 34.0 31.0 34.0 30.0 34.0 3 32.06276593827251 34.0 31.0 34.0 30.0 34.0 4 35.54346414018664 37.0 35.0 37.0 33.0 37.0 5 35.57426514730329 37.0 35.0 37.0 33.0 37.0 6 35.66677492523573 37.0 35.0 37.0 33.0 37.0 7 35.60474969740748 37.0 35.0 37.0 33.0 37.0 8 35.5897264813169 37.0 35.0 37.0 33.0 37.0 9 37.22528619776033 39.0 37.0 39.0 34.0 39.0 10-11 37.122368346257346 39.0 37.0 39.0 33.5 39.0 12-13 37.01934321825344 39.0 37.0 39.0 33.0 39.0 14-15 38.265253539951225 40.0 38.0 41.0 33.0 41.0 16-17 38.19411899476893 40.0 38.0 41.0 33.0 41.0 18-19 38.12114567143651 40.0 38.0 41.0 33.0 41.0 20-21 38.09734051329119 40.0 38.0 41.0 33.0 41.0 22-23 38.099483617023935 40.0 38.0 41.0 33.0 41.0 24-25 38.0155533595742 40.0 38.0 41.0 33.0 41.0 26-27 37.928477075756966 40.0 38.0 41.0 33.0 41.0 28-29 37.77854092173911 40.0 38.0 41.0 33.0 41.0 30-31 37.591890901704915 40.0 37.5 41.0 32.0 41.0 32-33 37.36704178203078 40.0 37.0 41.0 31.5 41.0 34-35 37.07944318816749 40.0 36.5 41.0 31.0 41.0 36-37 36.84140460467483 40.0 36.0 41.0 30.5 41.0 38-39 36.257105898370504 39.5 35.5 40.5 29.5 41.0 40-41 36.22670072247864 39.0 35.0 40.0 30.0 41.0 42-43 36.40902415471663 39.5 36.0 41.0 30.0 41.0 44-45 36.29966763348386 40.0 36.0 41.0 30.0 41.0 46-47 36.00137639736741 39.5 35.0 41.0 28.5 41.0 48-49 35.736120604436366 39.0 35.0 41.0 27.0 41.0 50-51 34.59249435368595 38.0 34.0 40.0 25.5 40.5 52-53 34.69529643668711 38.5 34.5 39.5 26.0 40.5 54-55 35.170198761346356 39.0 35.0 40.5 26.0 41.0 56-57 35.07887994321105 39.0 35.0 41.0 26.0 41.0 58-59 34.86492848695421 38.5 35.0 40.0 26.0 41.0 60-61 34.5622319820538 38.0 34.0 40.0 25.5 41.0 62-63 34.17708698720446 37.5 34.0 40.0 23.5 41.0 64-65 33.78383403788057 37.0 34.0 40.0 23.0 41.0 66-67 33.41926706753532 36.5 33.0 39.5 22.0 41.0 68-69 33.01822279405546 36.0 33.0 39.0 21.5 41.0 70-71 32.605756086165044 35.5 32.5 39.0 20.0 40.0 72-73 32.17061902873693 35.0 32.0 38.0 18.5 40.0 74-75 31.709950684004674 35.0 32.0 37.0 17.0 39.5 76-77 31.25997775442381 35.0 31.0 37.0 13.0 39.0 78-79 30.874213536734665 35.0 31.0 36.0 9.0 39.0 80-81 30.481159369553858 35.0 31.0 36.0 7.0 37.5 82-83 30.06702614981692 34.0 30.5 35.0 2.0 37.0 84-85 29.66025566815063 34.0 30.0 35.0 2.0 36.5 86-87 29.23714623017551 34.0 29.5 35.0 2.0 36.0 88-89 28.82662037775193 34.0 29.0 35.0 2.0 36.0 90-91 28.43794894955682 34.0 29.0 35.0 2.0 35.5 92-93 27.997132996518626 34.0 28.5 35.0 2.0 35.0 94-95 27.522383354569115 34.0 27.0 35.0 2.0 35.0 96-97 26.92687260750873 33.5 25.5 35.0 2.0 35.0 98-99 26.152784093823783 33.0 24.5 35.0 2.0 35.0 100-101 23.48768486566001 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2521389139920913 1101 2 0.35731596413947386 1101 3 0.3528101262402288 1101 4 0.22549610077309268 1101 5 0.176109011411981 1101 6 0.23606875511640624 1101 7 0.16762819662212536 1101 8 0.0857529547629241 1101 9 0.14291023568635808 1101 10-11 0.27269180350563005 1101 12-13 0.29246425119423947 1101 14-15 0.39317363953652773 1101 16-17 0.3991927117232237 1101 18-19 0.43102205889162093 1101 20-21 0.4814760630076478 1101 22-23 0.495215855521721 1101 24-25 0.49698732529399336 1101 26-27 0.5031045496024191 1101 28-29 0.5441525336740156 1101 30-31 0.5220270347897085 1101 32-33 0.5235814389086286 1101 34-35 0.5195445432849866 1101 36-37 0.37759622498422374 1101 38-39 0.7794576322299065 1101 40-41 0.6339330519835755 1101 42-43 0.5013078705393568 1101 44-45 0.45965286847602727 1101 46-47 0.45847629010789603 1101 48-49 0.3883211701691991 1101 50-51 0.4331463346753992 1101 52-53 0.4632914242002286 1101 54-55 0.4262243448036642 1101 56-57 0.43449509081104054 1101 58-59 0.5088456613037025 1101 60-61 0.47580429563294757 1101 62-63 0.5170001887828306 1101 64-65 0.27109343587356705 1101 66-67 0.264594142143487 1101 68-69 0.4618876473525617 1101 70-71 0.3974873549811093 1101 72-73 0.4874728219309574 1101 74-75 0.42515403322014933 1101 76-77 0.4325210095877132 1101 78-79 0.44375062325308434 1101 80-81 0.296719210206593 1101 82-83 0.2265561322008871 1101 84-85 0.2319974355540424 1101 86-87 0.2781283105104322 1101 88-89 0.056931047179130445 1101 90-91 0.20245090600822735 1101 92-93 0.30682531793440404 1101 94-95 0.21206638205131156 1101 96-97 0.269068012438229 1101 98-99 0.1875227797038228 1101 100-101 -0.11090026112783136 1102 1 0.32760209951528196 1102 2 0.29683726630596396 1102 3 0.40808502482043707 1102 4 0.28001113086774865 1102 5 0.25142571984294193 1102 6 0.3177306253763277 1102 7 0.36909466109295863 1102 8 0.34032018774825445 1102 9 0.495860753763651 1102 10-11 0.5545175171580681 1102 12-13 0.48105989538741056 1102 14-15 0.6629628283980935 1102 16-17 0.5896572015758963 1102 18-19 0.6048617701683412 1102 20-21 0.5751332335218393 1102 22-23 0.5106044922270456 1102 24-25 0.5572007748027872 1102 26-27 0.5730474354210031 1102 28-29 0.5360725987775794 1102 30-31 0.4853715881296594 1102 32-33 0.3889027347763019 1102 34-35 0.4549156116526447 1102 36-37 0.4586874882347587 1102 38-39 0.7961220754951412 1102 40-41 0.7007296514569674 1102 42-43 0.6747880741454821 1102 44-45 0.6584244367079037 1102 46-47 0.6586358996290897 1102 48-49 0.5938740632551927 1102 50-51 0.5593255049792276 1102 52-53 0.44173351702213637 1102 54-55 0.49599661303560794 1102 56-57 0.5907496775049808 1102 58-59 0.6462447391813839 1102 60-61 0.5287335552194676 1102 62-63 0.5350150141660919 1102 64-65 0.489282652188443 1102 66-67 0.39191593775653644 1102 68-69 0.34967111617967106 1102 70-71 0.5057149108578045 1102 72-73 0.4086406632864623 1102 74-75 0.34184407283730067 1102 76-77 0.3355793948218704 1102 78-79 0.2729183719090891 1102 80-81 0.30167885621335344 1102 82-83 0.1256796911247875 1102 84-85 0.2693033385511505 1102 86-87 0.3533802481379311 1102 88-89 0.21186266050100144 1102 90-91 0.23138644539154996 1102 92-93 0.17743301068548334 1102 94-95 0.1412022949660745 1102 96-97 0.024999517610680755 1102 98-99 0.09673684245543868 1102 100-101 -0.11332671506895409 1103 1 0.21364740446536956 1103 2 0.27200791842910377 1103 3 0.2873555277956612 1103 4 0.2344749654089071 1103 5 0.2257560978734432 1103 6 0.26883734239656576 1103 7 0.22157630643864223 1103 8 0.2498149165830199 1103 9 0.372789924788016 1103 10-11 0.365195231665588 1103 12-13 0.24271280383069183 1103 14-15 0.3928130866033257 1103 16-17 0.4215003329246443 1103 18-19 0.4301185054397081 1103 20-21 0.45187296709512026 1103 22-23 0.47331323999317476 1103 24-25 0.5000693573788695 1103 26-27 0.4262863147942184 1103 28-29 0.38372414173862524 1103 30-31 0.33797392524697756 1103 32-33 0.3709460391018595 1103 34-35 0.22133275041473865 1103 36-37 0.062392740360458276 1103 38-39 0.5666275559183944 1103 40-41 0.37068311807390586 1103 42-43 0.2403834625703567 1103 44-45 0.28429836523686447 1103 46-47 0.3075030106202803 1103 48-49 0.17338400455830794 1103 50-51 0.14129378588484087 1103 52-53 0.2555335232409348 1103 54-55 0.4593017461494995 1103 56-57 0.36755699257578556 1103 58-59 0.31628522471302745 1103 60-61 0.2905849640490672 1103 62-63 0.19793748510243603 1103 64-65 0.1872751457202284 1103 66-67 0.138488711431755 1103 68-69 0.14428820421880317 1103 70-71 0.07003669899408749 1103 72-73 0.08066445798607447 1103 74-75 0.0069878470944679805 1103 76-77 0.005347623884539132 1103 78-79 0.1309023978594439 1103 80-81 -0.005926007473469497 1103 82-83 -0.09250833781353407 1103 84-85 0.07623150307392734 1103 86-87 -0.04433936999271637 1103 88-89 -0.1135556602741481 1103 90-91 -0.14688381900005965 1103 92-93 -0.05939563396655245 1103 94-95 -0.05856638650653068 1103 96-97 -0.13154824678424504 1103 98-99 -0.11994548663344418 1103 100-101 -0.34894284214221116 1104 1 0.21671759991532724 1104 2 0.297985881118521 1104 3 0.20050462753958698 1104 4 0.23258220338790636 1104 5 0.2950290491278693 1104 6 0.19055441617935287 1104 7 0.2544673061591851 1104 8 0.3150069160811384 1104 9 0.3199179168491071 1104 10-11 0.4084477311095185 1104 12-13 0.38992762768619826 1104 14-15 0.3568772708407124 1104 16-17 0.3109524799084866 1104 18-19 0.3937241493637629 1104 20-21 0.46080994061498615 1104 22-23 0.42757534083782645 1104 24-25 0.5146226655419142 1104 26-27 0.5801070563291901 1104 28-29 0.5623001430845846 1104 30-31 0.5779345158742757 1104 32-33 0.5460488399619052 1104 34-35 0.4524993762082019 1104 36-37 0.4050029126552701 1104 38-39 0.797216871472834 1104 40-41 0.6791941310259588 1104 42-43 0.5280404441888251 1104 44-45 0.46352938343589045 1104 46-47 0.4053463312734067 1104 48-49 0.37378301732785957 1104 50-51 0.34000734932480015 1104 52-53 0.31131019015474237 1104 54-55 0.42090876170222913 1104 56-57 0.3829249369578065 1104 58-59 0.24775512017268397 1104 60-61 0.46409684674487295 1104 62-63 0.43866124022822106 1104 64-65 0.28120169657315586 1104 66-67 0.43602263143814923 1104 68-69 0.31575668812711655 1104 70-71 0.4733256136109105 1104 72-73 0.3796482123134055 1104 74-75 0.38490640771133044 1104 76-77 0.40373332244561055 1104 78-79 0.4396852413797099 1104 80-81 0.36608856144730595 1104 82-83 0.30697894111471413 1104 84-85 0.3192893082399202 1104 86-87 0.23798216144068007 1104 88-89 0.11937326597728415 1104 90-91 0.2051240771325773 1104 92-93 0.13626527147946632 1104 94-95 0.18166197113210103 1104 96-97 0.10110122081851003 1104 98-99 0.11715792447304096 1104 100-101 -0.1391949395078882 1105 1 0.04062046550006926 1105 2 0.08449112781082846 1105 3 0.04666900217296899 1105 4 0.09651309478245906 1105 5 -0.06164283164294915 1105 6 0.03872730718285311 1105 7 0.07820723098917881 1105 8 0.0999316374524497 1105 9 0.17790472951069347 1105 10-11 0.17569283745552156 1105 12-13 0.24002213332394717 1105 14-15 0.2275826956815763 1105 16-17 0.12918880917916198 1105 18-19 0.06166392646080965 1105 20-21 0.2766407212098301 1105 22-23 0.26435514270187355 1105 24-25 0.20449725547434383 1105 26-27 0.18141492745391474 1105 28-29 0.1667378345399726 1105 30-31 0.29607253755625607 1105 32-33 0.2269081895024172 1105 34-35 0.1892544653911017 1105 36-37 0.07646981395662777 1105 38-39 0.4404162675057677 1105 40-41 0.41814647628655166 1105 42-43 0.18413252744068842 1105 44-45 0.10490628712703653 1105 46-47 0.2722671855174781 1105 48-49 0.1127516566639315 1105 50-51 0.04622815577290851 1105 52-53 0.014631465872220417 1105 54-55 0.10662673298120495 1105 56-57 0.2214240353522996 1105 58-59 0.29118154699055765 1105 60-61 0.2953304168854345 1105 62-63 0.18799834292435236 1105 64-65 0.23910350847434358 1105 66-67 0.11896056187331538 1105 68-69 0.010416848026302716 1105 70-71 0.15484107125718083 1105 72-73 0.22353440362757482 1105 74-75 0.1816380877391417 1105 76-77 0.1856360359523137 1105 78-79 0.17181226999210608 1105 80-81 0.08489545492750139 1105 82-83 0.1102942423378579 1105 84-85 0.1763085243862612 1105 86-87 0.10418390382048415 1105 88-89 0.07887108257116893 1105 90-91 0.1926816495325845 1105 92-93 0.20404346566139608 1105 94-95 0.2473754343835317 1105 96-97 0.4248395162409615 1105 98-99 0.5141853419707054 1105 100-101 0.31988004582744267 1106 1 0.2378993823523743 1106 2 0.22855473496250767 1106 3 0.2843606234588876 1106 4 0.20898467837501755 1106 5 0.28574519074091853 1106 6 0.27871905755063864 1106 7 0.3073289768020544 1106 8 0.2325698667248588 1106 9 0.28913692504770694 1106 10-11 0.37024988091584987 1106 12-13 0.3796754000383942 1106 14-15 0.5177734839722774 1106 16-17 0.39435683584330405 1106 18-19 0.4091593443394004 1106 20-21 0.400996866434447 1106 22-23 0.45317449441135693 1106 24-25 0.44662182336617917 1106 26-27 0.4896851448825075 1106 28-29 0.43825728776347006 1106 30-31 0.4199899028766083 1106 32-33 0.40637014196028076 1106 34-35 0.3308226494822648 1106 36-37 0.39997257307513934 1106 38-39 0.7611329620771201 1106 40-41 0.5643515806946624 1106 42-43 0.43857169236233773 1106 44-45 0.39559376338732477 1106 46-47 0.34402823326222176 1106 48-49 0.32374150629987497 1106 50-51 0.19392818214893737 1106 52-53 0.3099896745412778 1106 54-55 0.29173381496917017 1106 56-57 0.463886983258881 1106 58-59 0.48478641085349494 1106 60-61 0.4633673153346436 1106 62-63 0.5143005100592077 1106 64-65 0.49776154152780094 1106 66-67 0.353300251879908 1106 68-69 0.3009407048537369 1106 70-71 0.34497125687908436 1106 72-73 0.3818255819621399 1106 74-75 0.49230021918085853 1106 76-77 0.6264484226299878 1106 78-79 0.6613476218943681 1106 80-81 0.7119610369126299 1106 82-83 0.6716096855768114 1106 84-85 0.6201680800979652 1106 86-87 0.6947863834775241 1106 88-89 0.685847100081947 1106 90-91 0.6769544278708715 1106 92-93 0.6215323102620616 1106 94-95 0.7422518660859545 1106 96-97 0.5630310253381019 1106 98-99 0.7521234935659855 1106 100-101 0.6572440784673823 1107 1 0.2391447597442209 1107 2 0.17432745568842023 1107 3 0.25753436435170585 1107 4 0.20650034688278396 1107 5 0.24345572214723177 1107 6 0.23152565298629213 1107 7 0.27552466682290344 1107 8 0.18203437421033897 1107 9 0.3037478131690392 1107 10-11 0.3159042030869017 1107 12-13 0.33686020974467823 1107 14-15 0.2877927834925842 1107 16-17 0.3179314642373612 1107 18-19 0.40277984244588794 1107 20-21 0.34054727062006407 1107 22-23 0.24716100895801674 1107 24-25 0.31769357919018404 1107 26-27 0.3987205295598457 1107 28-29 0.40970464555488206 1107 30-31 0.3221202492476536 1107 32-33 0.439838393236478 1107 34-35 0.5210118623548396 1107 36-37 0.42257440633192545 1107 38-39 0.819150230355028 1107 40-41 0.6650775608580588 1107 42-43 0.394024498587207 1107 44-45 0.5766899425418188 1107 46-47 0.8322368612743389 1107 48-49 0.5427145998731504 1107 50-51 0.5955659114758731 1107 52-53 0.7303861324419145 1107 54-55 0.7985381805345 1107 56-57 0.7896201216864895 1107 58-59 0.7567196659379576 1107 60-61 0.7402348934992986 1107 62-63 0.8143910661161371 1107 64-65 0.7182728898779658 1107 66-67 0.811599811464454 1107 68-69 0.8405662368443174 1107 70-71 0.8592223789169253 1107 72-73 0.8958433038232343 1107 74-75 0.9544739499446528 1107 76-77 0.8854712324790697 1107 78-79 0.9909812422911806 1107 80-81 0.8747826430863022 1107 82-83 0.8357126148694647 1107 84-85 0.8466188731150588 1107 86-87 1.075064877630723 1107 88-89 1.0440956809668727 1107 90-91 0.8773250517874054 1107 92-93 0.925501731441976 1107 94-95 1.0146531567722583 1107 96-97 1.1467233012560207 1107 98-99 1.3455345182563825 1107 100-101 1.1629782248576568 1108 1 0.10856394739428765 1108 2 0.08024706243510593 1108 3 0.22673082195083794 1108 4 0.07169901286691527 1108 5 0.16538552907905313 1108 6 0.02528069958345469 1108 7 0.2042594442760972 1108 8 0.2232349412025485 1108 9 0.2731731483576638 1108 10-11 0.3478296121642188 1108 12-13 0.32356438427802203 1108 14-15 0.3408191457930414 1108 16-17 0.3157822492898603 1108 18-19 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2629.0 4 2661.0 5 2967.0 6 3530.0 7 4062.0 8 5030.0 9 6100.0 10 7055.0 11 8708.0 12 11496.0 13 14962.0 14 15292.0 15 16313.0 16 20524.0 17 26475.0 18 23414.0 19 16645.0 20 16233.0 21 17201.0 22 18415.0 23 20288.0 24 22892.0 25 26054.0 26 30293.0 27 35373.0 28 42040.0 29 50011.0 30 61216.0 31 74677.0 32 93945.0 33 123127.0 34 168998.0 35 248923.0 36 391442.0 37 612763.0 38 551124.0 39 80218.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.03491468963813 18.543283723223073 14.171890323542838 23.249911263595965 2 15.186369201941583 20.056227805955732 40.43012604723941 24.327276944863275 3 19.223124240810023 24.3695728117445 29.746217853578514 26.66108509386696 4 12.757440488595096 16.278769287196887 36.55700090861349 34.40678931559453 5 13.987697923473123 37.28039139875937 33.669075044612136 15.062835633155371 6 32.308558761761866 35.37205580455359 17.49621413491421 14.82317129877034 7 28.954810299325718 32.88612406447699 20.22683699860756 17.932228637589734 8 26.699422406731184 34.96424753203838 19.663892090026796 18.672437971203642 9 27.10508758861088 16.757287943089096 20.098970652452365 36.03865381584766 10-11 25.423476153967094 26.461424407011908 26.774296103759077 21.340803335261917 12-13 26.07444034567551 23.83729797984618 27.60684278281867 22.48141889165964 14-15 22.996245321837613 25.0831591360509 26.05956635940262 25.861029182708872 16-17 22.517593537237918 28.579590802778874 26.52926704005219 22.373548619931015 18-19 22.3695566943446 26.78402553614963 29.19178509897219 21.654632670533584 20-21 23.09811285576324 26.03049755077645 29.096536228318985 21.774853365141325 22-23 22.732555111507708 25.92129705180777 29.0743106938373 22.27183714284722 24-25 22.54114565507196 26.133401542591976 29.116966202208154 22.208486600127912 26-27 22.38129911328291 26.46796870361246 28.976832575854644 22.17389960724999 28-29 22.110536187872356 26.46343851627675 29.30882243668225 22.117202859168653 30-31 22.273328142888264 26.30225923235227 29.089736545854283 22.33467607890518 32-33 22.01577890368494 26.454559090681823 29.064966972772677 22.46469503286056 34-35 22.752949840065718 26.390330789423654 28.685710827057914 22.171008543452714 36-37 22.689525747897797 26.167633378451775 28.719759456544814 22.423081417105617 38-39 22.39083248223575 26.486005936416525 28.92771452837937 22.195447052968355 40-41 22.452362754795928 26.30969461650784 29.012711098099143 22.225231530597096 42-43 22.339500573663035 26.321731818189733 28.668683917761616 22.670083690385617 44-45 22.410256615614223 26.744389509592555 28.367282305729308 22.478071569063914 46-47 22.487864246712725 26.871657009137508 28.00136157232004 22.63911717182973 48-49 22.74721765211492 26.823954590856598 27.903006844248424 22.525820912780066 50-51 22.58684677085992 27.0414341100524 27.776827836656715 22.59489128243096 52-53 22.804514893125738 27.003719341638742 27.661774121956583 22.529991643278933 54-55 22.338366665447943 26.98715638498462 27.77563630475773 22.898840644809717 56-57 22.533132839408196 27.398138817677577 27.593864050499405 22.47486429241482 58-59 22.586014031770915 26.83548343759593 27.86600904777053 22.712493482862627 60-61 22.583027588921574 26.814571993481394 28.12081509346795 22.48158532412908 62-63 22.54031199954448 26.890297276999586 28.15758538423056 22.411805339225367 64-65 22.28966127763665 26.71149021855095 28.101713349015135 22.897135154797272 66-67 22.289069660243076 27.080735347359102 27.806961130908192 22.823233861489626 68-69 22.279895348906066 26.99919153511841 27.977238979223394 22.74367413675213 70-71 22.248201429452376 26.916029904223105 27.94441641608032 22.8913522502442 72-73 22.384367720496314 26.890008827044433 27.763275002398746 22.962348450060503 74-75 22.259203035987042 27.110450363503098 27.573695426428042 23.05665117408182 76-77 22.42908451220406 26.820014892831335 27.707073294005625 23.04382730095898 78-79 22.29636384019809 27.031522112271894 27.688351740787393 22.983762306742623 80-81 22.25345899917715 27.341738482724722 27.58278002170018 22.822022496397953 82-83 22.43922337115083 27.16672394480268 27.40837152165897 22.985681162387518 84-85 22.302894251974248 26.93027460930706 27.43485056059255 23.331980578126142 86-87 22.27580508350639 26.9038774654616 27.690575776948588 23.12974167408342 88-89 22.218801821108226 26.778014652162618 27.748777883717352 23.254405643011808 90-91 22.155214464202157 26.932579301591858 27.85714124006689 23.055064994139094 92-93 22.28047303147947 26.605112340100046 27.815468681100125 23.298945947320355 94-95 22.092137096633746 26.577362935758853 27.88748691234423 23.44301305526317 96-97 22.19013633560638 26.35545407731182 27.903337851324927 23.551071735756874 98-99 22.06983731375175 26.35743259398548 27.667093375369163 23.905636716893607 100-101 22.1925013114983 25.954137866372907 28.267424123315838 23.585936698812958 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4128.0 1 2419.5 2 873.5 3 1028.5 4 893.0 5 855.5 6 1388.5 7 2717.5 8 4209.0 9 4860.5 10 4424.5 11 3908.0 12 4037.5 13 4321.5 14 4605.5 15 4803.0 16 4991.5 17 5126.0 18 5228.0 19 5486.5 20 5906.0 21 6627.5 22 7683.5 23 9211.5 24 11097.5 25 13443.5 26 16633.0 27 20318.0 28 23312.0 29 26772.5 30 31068.0 31 35283.0 32 40486.0 33 44968.0 34 49091.5 35 53461.0 36 58972.5 37 65832.0 38 71077.0 39 74493.0 40 76950.5 41 82539.5 42 89564.5 43 94374.0 44 98532.0 45 103481.5 46 104389.0 47 106377.5 48 118275.0 49 120633.0 50 112862.5 51 108047.5 52 103212.0 53 102209.0 54 102294.0 55 96559.0 56 85750.5 57 75856.0 58 67606.0 59 58186.0 60 49708.5 61 42003.0 62 34913.0 63 26984.5 64 20198.0 65 16019.0 66 12411.0 67 10304.0 68 7831.0 69 5178.5 70 3848.5 71 3050.0 72 2577.5 73 2125.5 74 1686.0 75 1404.5 76 1016.0 77 663.0 78 431.5 79 289.0 80 206.5 81 136.5 82 93.5 83 76.0 84 58.5 85 41.0 86 27.0 87 23.5 88 22.0 89 13.0 90 7.0 91 5.0 92 9.0 93 9.5 94 5.0 95 3.5 96 3.5 97 4.5 98 2.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29708138595000183 2 0.18557621518799555 3 0.18557621518799555 4 0.20644227449751618 5 0.22068803924205602 6 0.11424340778102979 7 0.13143537691312648 8 0.14377477411277653 9 0.14606241516664425 10-11 0.13361903428272748 12-13 0.12406986594423425 14-15 0.1384889368292186 16-17 0.11535256707987471 18-19 0.1432375250773985 20-21 0.16878285017892125 22-23 0.1732368179883455 24-25 0.1973437021241787 26-27 0.17743082658710296 28-29 0.17573242641074663 30-31 0.1655246947385642 32-33 0.1739993650063014 34-35 0.35678535133314015 36-37 0.4761239596432389 38-39 0.44354240523966854 40-41 0.43324802049726385 42-43 0.45659235761514116 44-45 0.46038776209087623 46-47 0.46404452165425564 48-49 0.47666120867861694 50-51 0.4694863344642137 52-53 0.4757773473623499 54-55 0.45797880673869734 56-57 0.42158451724534746 58-59 0.4115674223276539 60-61 0.36486141747785494 62-63 0.4721379184130149 64-65 0.4709247754299032 66-67 0.48017932332964075 68-69 0.4920161327220017 70-71 0.4841653645598648 72-73 0.4775797312229729 74-75 0.4821203521026194 76-77 0.4581001210370085 78-79 0.44581271567949177 80-81 0.44163603769477877 82-83 0.45465132884216247 84-85 0.4558471412112297 86-87 0.4572509209488303 88-89 0.46480706867221155 90-91 0.482640270523953 92-93 0.48712889956146616 94-95 0.4867129648243993 96-97 0.48368010736662015 98-99 0.4806299192947965 100-101 0.4628140480570996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2885068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.939020911593694 #Duplication Level Percentage of deduplicated Percentage of total 1 71.97138963296658 34.50237952651242 2 14.516407234889078 13.918046999891152 3 5.472321415171431 7.870131922705956 4 2.6481063989346456 5.077905121386123 5 1.5080775861633824 3.6147881469696066 6 0.8948814754190204 2.5739845058106146 7 0.5945323803702149 1.995091015063112 8 0.39317510027264857 1.5078743483110757 9 0.3129528088768177 1.350238612417897 >10 1.4852751214767872 12.851476007272197 >50 0.11812322330805988 3.9270128895818943 >100 0.07759209251344758 7.06786945974573 >500 0.00579032698010415 1.9459928606407644 >1k 0.0011580653959898219 0.8471821140514273 >5k 1.4475817449872774E-4 0.5476357841609468 >10k+ 7.237908724936387E-5 0.4023906854789648 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11597 0.4019662621470274 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9396 0.32567689912334824 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6387 0.22138126380383408 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.466122808890466E-5 2 0.0 0.0 0.0 0.0 3.466122808890466E-5 3 0.0 0.0 0.0 0.0 3.466122808890466E-5 4 0.0 0.0 0.0 0.0 3.466122808890466E-5 5 0.0 0.0 0.0 0.0 3.466122808890466E-5 6 0.0 0.0 0.0 0.0 6.932245617780932E-5 7 0.0 0.0 0.0 0.0 6.932245617780932E-5 8 0.0 0.0 0.0 0.0 6.932245617780932E-5 9 0.0 0.0 0.0 0.0 6.932245617780932E-5 10-11 0.0 0.0 0.0 0.0 6.932245617780932E-5 12-13 0.0 0.0 0.0 0.0 1.9063675448897566E-4 14-15 0.0 0.0 0.0 5.1991842133357E-5 2.07967368533428E-4 16-17 0.0 0.0 0.0 6.932245617780932E-5 2.07967368533428E-4 18-19 0.0 0.0 0.0 1.03983684266714E-4 2.4262859662233264E-4 20-21 0.0 0.0 0.0 1.03983684266714E-4 3.6394289493349896E-4 22-23 0.0 0.0 0.0 1.9063675448897566E-4 4.852571932446653E-4 24-25 0.0 0.0 0.0 4.332653511113083E-4 4.852571932446653E-4 26-27 0.0 0.0 0.0 8.665307022226165E-4 5.025878072891176E-4 28-29 0.0 0.0 0.0 0.0025822614926233976 5.1991842133357E-4 30-31 0.0 0.0 0.0 0.007400172196981146 5.372490353780223E-4 32-33 0.0 0.0 0.0 0.015649544482140454 5.545796494224746E-4 34-35 0.0 0.0 0.0 0.029843317384546916 5.892408775113793E-4 36-37 0.0 0.0 0.0 0.05434880564340251 5.892408775113793E-4 38-39 0.0 0.0 0.0 0.09235484224288648 6.239021056002839E-4 40-41 0.0 0.0 0.0 0.13325509138779398 6.239021056002839E-4 42-43 0.0 0.0 0.0 0.17597505500736899 6.412327196447362E-4 44-45 0.0 0.0 0.0 0.23009856266819362 6.932245617780933E-4 46-47 0.0 0.0 0.0 0.297202700248313 7.452164039114502E-4 48-49 0.0 0.0 0.0 0.3746185531848816 8.31869474133712E-4 50-51 0.0 0.0 0.0 0.46050907638918737 8.31869474133712E-4 52-53 0.0 0.0 0.0 0.5447531912592701 9.011919303115213E-4 54-55 0.0 0.0 0.0 0.6403661889425136 9.011919303115213E-4 56-57 0.0 0.0 0.0 0.7556840947942995 9.011919303115213E-4 58-59 0.0 0.0 0.0 0.8891298229365825 9.011919303115213E-4 60-61 0.0 0.0 0.0 1.038155773104828 9.35853158400426E-4 62-63 0.0 0.0 0.0 1.191063780819031 9.705143864893306E-4 64-65 0.0 0.0 0.0 1.3486683849392804 9.705143864893306E-4 66-67 0.0 0.0 0.0 1.5358736778474547 9.705143864893306E-4 68-69 0.0 0.0 0.0 1.751449185946397 9.705143864893306E-4 70-71 0.0 0.0 0.0 1.9954815623063304 9.705143864893306E-4 72-73 0.0 0.0 0.0 2.249531033583957 0.0010051756145782352 74-75 0.0 0.0 0.0 2.5086757053906528 0.00103983684266714 76-77 0.0 0.0 0.0 2.793019090017982 0.00103983684266714 78-79 0.0 0.0 0.0 3.1127342579100388 0.00103983684266714 80-81 0.0 0.0 0.0 3.4750654057374035 0.00103983684266714 82-83 0.0 0.0 0.0 3.8502038773436187 0.0011091592988449492 84-85 0.0 1.733061404445233E-5 0.0 4.228461859477836 0.0011091592988449492 86-87 0.0 3.466122808890466E-5 0.0 4.634379501626998 0.001195812369067211 88-89 0.0 3.466122808890466E-5 0.0 5.072549416512887 0.0012824654392894726 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14760 0.0 32.987988 1 GGTATCA 9705 0.0 32.713375 1 TATCAAC 18175 0.0 26.715176 2 ATCAACG 18265 0.0 26.609497 3 TCAACGC 18405 0.0 26.328886 4 CAACGCA 18600 0.0 26.026913 5 GTACATG 14005 0.0 25.447882 1 AACGCAG 19080 0.0 25.330576 6 TACATGG 13840 0.0 25.113104 2 ACATGGG 14260 0.0 23.60866 3 ACGCAGA 21010 0.0 22.890923 7 CGCAGAG 21170 0.0 22.695532 8 GAGTACT 10250 0.0 22.326088 12-13 CATGGGG 6650 0.0 21.746813 4 AGTACTT 10755 0.0 20.92534 12-13 GCAGAGT 22520 0.0 20.913475 9 GTACTTT 11000 0.0 20.85109 14-15 ATGGGGA 4015 0.0 18.894886 5 AGAGTAC 19755 0.0 18.862356 10-11 CATGGGA 7750 0.0 18.84371 4 >>END_MODULE